##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2852210 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.358411898142144 33.0 33.0 33.0 22.0 33.0 2 29.494588757489804 33.0 33.0 33.0 15.0 33.0 3 29.2555151969876 33.0 33.0 33.0 15.0 33.0 4 32.329446639623306 37.0 37.0 37.0 15.0 37.0 5 32.439927284456616 37.0 37.0 37.0 15.0 37.0 6 33.47195157439319 37.0 37.0 37.0 15.0 37.0 7 32.56059581868095 37.0 37.0 37.0 15.0 37.0 8 33.07218963540553 37.0 37.0 37.0 15.0 37.0 9 32.01033654604675 37.0 37.0 37.0 6.0 37.0 10-11 32.09800856178192 37.0 33.0 37.0 15.0 37.0 12-13 32.109592912162846 37.0 33.0 37.0 10.5 37.0 14-15 33.524089390332406 37.0 33.0 40.0 10.5 40.0 16-17 33.3303490977172 37.0 33.0 40.0 10.5 40.0 18-19 33.50333022463283 37.0 33.0 40.0 10.5 40.0 20-21 33.47923066674613 37.0 33.0 40.0 10.5 40.0 22-23 34.866961058267094 37.0 33.0 40.0 18.5 40.0 24-25 33.87026796764614 37.0 33.0 40.0 15.0 40.0 26-27 33.615497456358405 37.0 33.0 40.0 10.5 40.0 28-29 33.08673028984542 37.0 33.0 40.0 6.0 40.0 30-31 33.09931789734978 37.0 33.0 40.0 10.5 40.0 32-33 32.81987055651582 37.0 33.0 40.0 6.0 40.0 34-35 33.243518534750244 37.0 33.0 40.0 10.5 40.0 36-37 32.84439995652494 37.0 33.0 40.0 6.0 40.0 38-39 32.72561259514552 37.0 33.0 40.0 6.0 40.0 40-41 32.53699464625676 37.0 33.0 40.0 6.0 40.0 42-43 32.4076640569944 37.0 33.0 40.0 6.0 40.0 44-45 32.24172185778747 37.0 33.0 40.0 6.0 40.0 46-47 32.18661160994457 37.0 33.0 40.0 6.0 40.0 48-49 32.08388810781815 37.0 33.0 40.0 6.0 40.0 50-51 31.269399342965627 37.0 30.0 38.5 10.5 38.5 52-53 31.725566841151245 37.0 33.0 38.5 10.5 40.0 54-55 32.235115577043764 37.0 33.0 40.0 6.0 40.0 56-57 32.69169643890176 37.0 33.0 40.0 10.5 40.0 58-59 31.89180863260419 37.0 33.0 40.0 6.0 40.0 60-61 31.73376995382528 37.0 33.0 40.0 6.0 40.0 62-63 31.255843363567198 37.0 33.0 37.0 6.0 40.0 64-65 31.055513268658338 37.0 33.0 37.0 6.0 40.0 66-67 30.963073721780653 37.0 33.0 37.0 6.0 40.0 68-69 31.276502957355873 37.0 33.0 37.0 10.5 38.5 70-71 30.821265264479123 37.0 27.0 37.0 10.5 37.0 72-73 31.449813477969712 37.0 27.0 37.0 15.0 37.0 74-75 31.79818561746856 37.0 27.0 37.0 22.0 37.0 76-77 31.45514022459777 33.0 27.0 37.0 22.0 37.0 78-79 30.89761220246756 33.0 27.0 37.0 15.0 37.0 80-81 30.279546211534214 33.0 27.0 37.0 15.0 37.0 82-83 29.655354444448342 33.0 27.0 37.0 6.0 37.0 84-85 29.423722481864942 33.0 27.0 37.0 6.0 37.0 86-87 29.203919767478553 33.0 27.0 37.0 6.0 37.0 88-89 29.031998695748207 33.0 27.0 37.0 6.0 37.0 90-91 28.98187861342608 33.0 27.0 37.0 6.0 37.0 92-93 28.7692775076169 33.0 27.0 37.0 6.0 37.0 94-95 28.57710073942662 33.0 27.0 37.0 6.0 37.0 96-97 28.42830173795057 33.0 22.0 37.0 6.0 37.0 98-99 28.312957846722366 33.0 22.0 37.0 6.0 37.0 100-101 27.10428650064336 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 -0.13347273763650946 2306 2 0.15201509070093167 2306 3 -0.15003166131355883 2306 4 0.08787885826147601 2306 5 0.05156962452944924 2306 6 -0.455875474721978 2306 7 0.5599871058090429 2306 8 -0.40231201426443164 2306 9 0.14856276756777476 2306 10-11 -0.028019052940400968 2306 12-13 0.03213554240527117 2306 14-15 0.5524224747804993 2306 16-17 0.3561427655511622 2306 18-19 0.43739271369896215 2306 20-21 0.25379569623177645 2306 22-23 -0.04675086808672546 2306 24-25 0.5687225378959155 2306 26-27 -0.06471526363074531 2306 28-29 0.13824267935954992 2306 30-31 0.27791999205627604 2306 32-33 0.2966415818416124 2306 34-35 -0.01824120066448387 2306 36-37 0.4680095945532301 2306 38-39 0.19341733140983308 2306 40-41 0.21037555477783343 2306 42-43 0.23166947210611255 2306 44-45 0.275059368980223 2306 46-47 0.27793796874073706 2306 48-49 0.29598520241791704 2306 50-51 0.12916254968795116 2306 52-53 0.08211743649096448 2306 54-55 0.27292698331434195 2306 56-57 0.16833748259583103 2306 58-59 0.5746293858019271 2306 60-61 0.3272155595334105 2306 62-63 0.5214336108176276 2306 64-65 0.5297805330655692 2306 66-67 0.2836535361480088 2306 68-69 -0.07035460961893492 2306 70-71 0.2243711342271375 2306 72-73 -0.34217444548881915 2306 74-75 -0.2108930037174801 2306 76-77 0.01674998257367477 2306 78-79 0.19060383548087145 2306 80-81 0.42307584112761276 2306 82-83 0.5485677563381728 2306 84-85 0.5467115387065853 2306 86-87 0.4488571960355934 2306 88-89 0.5627607375887358 2306 90-91 0.5553481107172153 2306 92-93 0.4929047720482025 2306 94-95 0.2739945710099576 2306 96-97 0.35934857212748383 2306 98-99 0.3306820425419481 2306 100-101 0.18288783278384102 2307 1 0.031543213356901134 2307 2 0.049624849819142725 2307 3 -0.06362108205512484 2307 4 0.12035363596815785 2307 5 0.05966839454845996 2307 6 -0.2236382991601431 2307 7 0.626015732739269 2307 8 -0.299262977860387 2307 9 0.20553109263319413 2307 10-11 0.12767055517659998 2307 12-13 0.015392159602065192 2307 14-15 0.36879942049207415 2307 16-17 0.27063982189910973 2307 18-19 0.26066092432088084 2307 20-21 0.2269424683057224 2307 22-23 -0.018035353079490335 2307 24-25 0.39313518355910304 2307 26-27 -0.2125219733685384 2307 28-29 0.030155491298366144 2307 30-31 0.21517186867746574 2307 32-33 0.25379521823235507 2307 34-35 -0.10615800780539786 2307 36-37 0.4110438496269282 2307 38-39 -0.09302283344386808 2307 40-41 0.03949380083366805 2307 42-43 0.11456670459156015 2307 44-45 0.20850210021229287 2307 46-47 0.02992180904274022 2307 48-49 -0.11193450594609189 2307 50-51 -0.09156749207589954 2307 52-53 -0.1236111030585576 2307 54-55 -0.06355125292549957 2307 56-57 0.05469353491417195 2307 58-59 0.21642408470542307 2307 60-61 0.1877662070909949 2307 62-63 0.27274059462352795 2307 64-65 0.10278700290775689 2307 66-67 0.0838346619143131 2307 68-69 -0.08225507129182574 2307 70-71 0.2028616611030145 2307 72-73 -0.6049770958077225 2307 74-75 -0.41941153370328266 2307 76-77 -0.1969064644486167 2307 78-79 0.05884100416233906 2307 80-81 0.17555420809449274 2307 82-83 0.2464515262310485 2307 84-85 0.38659699199689257 2307 86-87 0.29045786847718347 2307 88-89 0.35639261021930935 2307 90-91 0.3279180210201069 2307 92-93 0.28135859214485137 2307 94-95 0.07281080318119493 2307 96-97 0.3312634711121696 2307 98-99 0.19580526998637282 2307 100-101 0.25498810043027476 2201 1 0.08329549579838869 2201 2 -0.0315805928947519 2201 3 -0.1383056495875472 2201 4 0.13154798526393563 2201 5 0.11234780197429473 2201 6 -0.11066891284875169 2201 7 0.7440048231600898 2201 8 -0.22132608327850534 2201 9 0.21922060489227846 2201 10-11 0.10892430066962078 2201 12-13 0.12382063409035737 2201 14-15 0.5042032848946434 2201 16-17 0.40240349514522933 2201 18-19 0.370969422275671 2201 20-21 0.22720894464502805 2201 22-23 -0.036475299477821466 2201 24-25 0.44012865096869547 2201 26-27 0.0688724449569662 2201 28-29 0.3705266116434771 2201 30-31 0.3871250762613556 2201 32-33 0.424900243433612 2201 34-35 0.12043465467802861 2201 36-37 0.6023553872323575 2201 38-39 0.3804956184881121 2201 40-41 0.4140514834537683 2201 42-43 0.43390430767428967 2201 44-45 0.4979547918756495 2201 46-47 0.3318455393149762 2201 48-49 0.42833559310164304 2201 50-51 0.3715772171026188 2201 52-53 0.23833198437217717 2201 54-55 0.558619452340146 2201 56-57 0.5192496585080093 2201 58-59 0.6879029157421286 2201 60-61 0.566607607258792 2201 62-63 0.7093842904349721 2201 64-65 0.7039726739243761 2201 66-67 0.6360171635116387 2201 68-69 0.3836221693578423 2201 70-71 0.5303121485014834 2201 72-73 0.15687515356077952 2201 74-75 0.31874441758660765 2201 76-77 0.4106511181414767 2201 78-79 0.6044336909773946 2201 80-81 0.9012469609653984 2201 82-83 0.8919916747620924 2201 84-85 0.7727561730845522 2201 86-87 0.8445549083999708 2201 88-89 0.8186325434605415 2201 90-91 0.7199322919680604 2201 92-93 0.7564041688809375 2201 94-95 0.7694587164741016 2201 96-97 0.588789918235495 2201 98-99 0.5702202070801157 2201 100-101 0.7672999921960013 1101 1 -0.13419584169294652 1101 2 -0.4363543176684779 1101 3 -0.40569303697493453 1101 4 -0.3089064551905025 1101 5 -0.3229389533124589 1101 6 -0.31510747728731303 1101 7 0.3319415710968414 1101 8 -0.5544284163808371 1101 9 -0.22932724365557178 1101 10-11 -0.25590906416374537 1101 12-13 -0.2666138563441294 1101 14-15 0.038135958827311356 1101 16-17 -0.08190276916104011 1101 18-19 -0.18242691112065756 1101 20-21 -0.2247925673564879 1101 22-23 -0.29175407475659654 1101 24-25 0.10847321931326803 1101 26-27 -0.26657433048981716 1101 28-29 -0.18068949957262959 1101 30-31 0.0031123922486742117 1101 32-33 -0.027815689282320477 1101 34-35 -0.29683828259489786 1101 36-37 0.09573366061062671 1101 38-39 -0.22285010485761347 1101 40-41 -0.18320549380320728 1101 42-43 -0.08052228675230566 1101 44-45 0.012144506065361327 1101 46-47 -0.12277551530608122 1101 48-49 -0.08609352132747716 1101 50-51 -0.14886294333754435 1101 52-53 -0.22844934240915293 1101 54-55 -0.10996703624635273 1101 56-57 0.17767129692963834 1101 58-59 0.20559459343380126 1101 60-61 -0.024593803942618564 1101 62-63 -0.0409657799150942 1101 64-65 -0.03194786199615862 1101 66-67 -0.001591774097299492 1101 68-69 -0.2715931858575118 1101 70-71 0.02379794198727936 1101 72-73 -0.3822898856042549 1101 74-75 -0.029559830717641233 1101 76-77 0.01695280444316083 1101 78-79 -0.04720200065829516 1101 80-81 0.3111959309143657 1101 82-83 0.3188780016484216 1101 84-85 0.28414700447538266 1101 86-87 0.22256410640916968 1101 88-89 0.2882681930961901 1101 90-91 0.1879198599556311 1101 92-93 0.1359799684567342 1101 94-95 0.17158475860014377 1101 96-97 0.2023580318036089 1101 98-99 0.06556660242651091 1101 100-101 0.14098066587667546 2304 1 0.3590901356814271 2304 2 0.2971084871755423 2304 3 0.2145069104073336 2304 4 0.38312036390814086 2304 5 0.431954416056044 2304 6 0.33973630385480647 2304 7 0.8806239752488594 2304 8 0.35945389529153005 2304 9 0.6591399061375469 2304 10-11 0.48400507326420694 2304 12-13 0.4596619243515434 2304 14-15 0.7700272474036822 2304 16-17 0.7186545801808464 2304 18-19 0.6986945640892159 2304 20-21 0.6789944743311054 2304 22-23 0.538622620371477 2304 24-25 0.6834718241461246 2304 26-27 0.7267833186136912 2304 28-29 0.7895844315300238 2304 30-31 0.8724382474861301 2304 32-33 0.8671322909142773 2304 34-35 0.6465716653454336 2304 36-37 0.9982221604471917 2304 38-39 0.7762098713846868 2304 40-41 0.7945018081029858 2304 42-43 0.7531818421451888 2304 44-45 0.7687233170640653 2304 46-47 0.6987068697122183 2304 48-49 0.8385263952813915 2304 50-51 0.7374271876487235 2304 52-53 0.6287925317387995 2304 54-55 0.8486721575862148 2304 56-57 0.8435143366400908 2304 58-59 0.9936589784042695 2304 60-61 0.784172549409373 2304 62-63 0.8718884131600895 2304 64-65 0.8197578294885375 2304 66-67 0.9373302001837907 2304 68-69 0.7736301604708018 2304 70-71 0.776310438698669 2304 72-73 0.45833857739989625 2304 74-75 0.6978213209729063 2304 76-77 0.9361047819929276 2304 78-79 1.0333998345844222 2304 80-81 0.9334008530640432 2304 82-83 1.0516919341861097 2304 84-85 0.9147299451688227 2304 86-87 0.7753892002839855 2304 88-89 0.6952331863595269 2304 90-91 0.6553096218205319 2304 92-93 0.7594403815856587 2304 94-95 0.8061133116922328 2304 96-97 0.8896719762998728 2304 98-99 0.7167317662988566 2304 100-101 0.7431168660791698 2202 1 -0.04025414334060429 2202 2 -0.344743917084692 2202 3 -0.25243574926897594 2202 4 -0.1273225553556543 2202 5 -0.2798863742902995 2202 6 -0.14201994800206563 2202 7 0.3915728650247132 2202 8 -0.28100213839181976 2202 9 -0.13488087886700129 2202 10-11 -0.1969506588175065 2202 12-13 -0.10733475352890665 2202 14-15 -0.05184637400672898 2202 16-17 -0.1852964012999152 2202 18-19 -0.18408263182580953 2202 20-21 -0.10516933887394231 2202 22-23 -0.18918234990350413 2202 24-25 0.007140500490052659 2202 26-27 0.0025665075483658484 2202 28-29 -0.015966989298789258 2202 30-31 0.0941616682789359 2202 32-33 -0.07095084310188327 2202 34-35 -0.1876797773907697 2202 36-37 0.12515563702880428 2202 38-39 -0.126772983837526 2202 40-41 -0.10189082621477752 2202 42-43 -0.07693930868263976 2202 44-45 0.0823350042026263 2202 46-47 -0.12585190659540757 2202 48-49 -0.02798006739652692 2202 50-51 0.04205459708570203 2202 52-53 -0.06762321036628904 2202 54-55 0.08465638427060895 2202 56-57 0.24068393279399203 2202 58-59 0.17752569148657216 2202 60-61 0.0051584716651724705 2202 62-63 0.07578192527367378 2202 64-65 0.09709734290455785 2202 66-67 0.06572396888384091 2202 68-69 0.05105535276060991 2202 70-71 0.10351910802023312 2202 72-73 -0.05181594467404338 2202 74-75 0.3439240575951459 2202 76-77 0.17754004567678905 2202 78-79 0.23534283937704004 2202 80-81 0.337234645051943 2202 82-83 0.1644784932565173 2202 84-85 0.1328907153483705 2202 86-87 0.10586056913529163 2202 88-89 0.3391423337421955 2202 90-91 0.41085068916022394 2202 92-93 0.32114492601712996 2202 94-95 0.2300450266801839 2202 96-97 0.1470367582313834 2202 98-99 0.18254244449741108 2202 100-101 0.12982538685067269 1102 1 -0.042747006839856994 1102 2 -0.3417792355402014 1102 3 -0.23301319908232898 1102 4 -0.14417018673083248 1102 5 -0.17054940432716847 1102 6 0.03558761957799561 1102 7 0.11535503706349459 1102 8 0.05317881090724086 1102 9 -0.1548913958154685 1102 10-11 -0.08951526292951684 1102 12-13 -0.07064504973702412 1102 14-15 0.0015340737147937489 1102 16-17 -0.007984406200115757 1102 18-19 -0.03273984286975917 1102 20-21 -0.10735059443579331 1102 22-23 0.10818927976122694 1102 24-25 0.0849027885619833 1102 26-27 0.11762741339278193 1102 28-29 0.0238809772318902 1102 30-31 0.11755046599525798 1102 32-33 0.03825712088622879 1102 34-35 -0.0171107642503614 1102 36-37 0.046497530310666946 1102 38-39 -0.0014774113572570968 1102 40-41 0.0016904258799428362 1102 42-43 0.01550047889811168 1102 44-45 0.10334795391774776 1102 46-47 0.042584390745311396 1102 48-49 0.008056949950656644 1102 50-51 0.044274279959253704 1102 52-53 0.04719586344882387 1102 54-55 0.256507511664374 1102 56-57 0.5311864637958692 1102 58-59 0.33531458974954376 1102 60-61 0.20444828871649534 1102 62-63 0.3106846146076414 1102 64-65 0.3714631477978294 1102 66-67 0.3288029789038376 1102 68-69 0.280681076523134 1102 70-71 0.2770020959893067 1102 72-73 0.31094105603094135 1102 74-75 0.7060949695648162 1102 76-77 0.54672061937055 1102 78-79 0.5463723446181774 1102 80-81 0.47387277534653194 1102 82-83 0.4086868039572238 1102 84-85 0.39039704285308474 1102 86-87 0.34399866475861884 1102 88-89 0.3529006115052091 1102 90-91 0.34763862818503455 1102 92-93 0.37879167211950104 1102 94-95 0.5196674855658472 1102 96-97 0.5441655446217588 1102 98-99 0.5316698844872398 1102 100-101 0.4155232037170862 2305 1 0.25639696685904667 2305 2 0.1569956543549651 2305 3 0.20523504854498853 2305 4 0.12867268902129325 2305 5 0.22126872109929252 2305 6 0.45579263167401507 2305 7 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-0.2691048231742066 1213 66-67 -0.07834804159151787 1213 68-69 0.4144616395045446 1213 70-71 -0.17457053190830507 1213 72-73 0.3518337300705632 1213 74-75 -0.22238872204050608 1213 76-77 -0.2900619211257798 1213 78-79 -0.5119517572604622 1213 80-81 -1.0461575740144369 1213 82-83 -1.2872039017226413 1213 84-85 -1.1402632121486889 1213 86-87 -1.1723272773075166 1213 88-89 -0.983804491581914 1213 90-91 -1.087466534105463 1213 92-93 -1.1262291711319214 1213 94-95 -0.8421605255668254 1213 96-97 -0.9069321592636932 1213 98-99 -1.0721973320994742 1213 100-101 -1.0701859703652623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2118.0 3 410.0 4 534.0 5 357.0 6 620.0 7 4978.0 8 28919.0 9 96441.0 10 122830.0 11 75665.0 12 40558.0 13 25077.0 14 17162.0 15 13571.0 16 11492.0 17 10152.0 18 9772.0 19 9960.0 20 10032.0 21 10701.0 22 11195.0 23 11797.0 24 13083.0 25 14639.0 26 17481.0 27 20921.0 28 27442.0 29 34509.0 30 40615.0 31 52556.0 32 73145.0 33 107016.0 34 163937.0 35 286723.0 36 538578.0 37 787857.0 38 159319.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.072637810636785 18.961524590475108 21.98467282650036 11.981164772387748 2 34.21955891397944 25.45713030489412 30.13243124505755 10.190879536068886 3 39.49656919406802 17.675343519764464 29.863119099734035 12.964968186433474 4 29.75305540574488 14.271524865741334 35.73667909851257 20.23874063000121 5 38.30116497628618 12.571504271720274 31.875329651427762 17.252001100565778 6 36.14103716531294 12.644612010044565 37.11748669404347 14.096864130599027 7 41.9686568878855 16.592660047000262 25.845648154296335 15.593034910817897 8 29.156717254239705 35.64099978815963 20.08756068453597 15.11472227306469 9 37.92141530703962 27.4745762533267 18.5703552773878 16.03365316224588 10-11 32.33922053493004 30.01454328092344 22.878835610352454 14.767400573794069 12-13 35.94667356148895 21.557654877492485 22.53953471542435 19.956136845594212 14-15 30.80708070174699 21.79376793983982 24.950283963666983 22.44886739474621 16-17 33.003247694207374 23.424323806880757 26.254683160995352 17.317745337916524 18-19 32.59393793778604 23.51542211698321 26.813994259626334 17.07664568560442 20-21 29.412872241471856 28.021050242938756 25.61607596999868 16.95000154559071 22-23 35.14386403787073 23.36834172989399 24.161447127652888 17.32634710458239 24-25 29.08501157848707 29.05265979395242 24.767871102882374 17.09445752467814 26-27 23.708841668231855 32.69718047797022 25.462786121938468 18.131191731859452 28-29 26.075591748168602 30.750636950848527 23.91964452394538 19.254126777037495 30-31 29.920491221162028 25.651187973308392 25.31137276134358 19.116948044185996 32-33 25.09598603225609 27.902126605893102 27.2494637936747 19.752423568176113 34-35 32.55642596348742 23.97119974789316 24.345139469457195 19.127234819162233 36-37 26.480479895394787 25.294175778534054 27.656444470859903 20.56889985521125 38-39 19.919578538353477 30.01028748860928 29.42448633350494 20.645647639532307 40-41 22.891936702078823 31.648872642536592 25.359606503997544 20.09958415138705 42-43 28.642630824462316 25.722149483557434 27.143494861264074 18.491724830716176 44-45 23.534248922237328 29.449107562025112 27.396569698954664 19.620073816782895 46-47 26.356482081802124 30.700085560597856 24.495191592927906 18.44824076467211 48-49 21.159840006782773 28.284583036279216 29.4739604688908 21.08161648804721 50-51 18.586236551269852 25.50307821798344 30.91040372933076 25.000281501415948 52-53 27.557684188304833 25.614559144324815 24.772623319963408 22.055133347406937 54-55 30.975091043462015 25.217904563446908 25.247927009050354 18.559077384040723 56-57 32.4376245880786 24.889922638830488 23.414289559848903 19.258163213242007 58-59 28.68855588079191 24.335687783417566 23.531330323817077 23.444426011973444 60-61 21.832018298059577 24.004649080260823 24.750777572566697 29.4125550491129 62-63 26.380159267675328 23.702583123927916 25.531003605781912 24.386254002614844 64-65 23.236080044633745 28.01089187130979 28.233744087796108 20.51928399626036 66-67 20.779296143437197 27.45997218055558 26.476092335233098 25.284639340774127 68-69 19.168722774523374 23.426598268472876 36.37777398699684 21.026904970006914 70-71 18.629392760598666 31.620175380760863 30.54524515944704 19.205186699193426 72-73 20.397136034828968 37.6601045853686 24.148283344059134 17.794476035743298 74-75 21.53086138426194 36.9202687937793 23.752376772667123 17.796493049291637 76-77 20.689054302527605 35.85663276044604 23.87291224771885 19.581400689307504 78-79 20.604684482169258 36.3252923493309 24.238249406720414 18.83177376177943 80-81 19.78618804604477 36.89348339236013 24.185023947797074 19.135304613798027 82-83 19.7165327904407 35.68117159551403 24.470589973583586 20.131705640461682 84-85 20.5120168402153 34.2183311118686 24.600145142731307 20.66950690518479 86-87 19.484641620215402 34.11913803808857 25.404640494479498 20.991579847216528 88-89 19.279743147588828 34.191929425388764 25.51142883475767 21.016898592264738 90-91 20.273938975752465 32.80527641924307 24.873907288820757 22.046877316183703 92-93 20.926255449618946 32.70144096566272 24.034883187350854 22.33742039736748 94-95 21.254241692840516 33.90722088698437 23.520170316528443 21.318367103646672 96-97 20.684558500607775 34.45862293638596 22.776557070217464 22.0802614927888 98-99 20.828957069799888 32.50270928298911 24.484849380043254 22.183484267167746 100-101 22.004684807897547 33.423033439496045 23.759342002233005 20.8129397503734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1999.0 1 1298.5 2 507.5 3 522.5 4 545.0 5 424.0 6 433.5 7 557.5 8 697.5 9 810.5 10 879.5 11 1022.0 12 1140.0 13 1059.0 14 953.5 15 923.5 16 869.0 17 808.0 18 764.0 19 728.0 20 722.0 21 755.0 22 777.5 23 851.5 24 1020.0 25 1171.5 26 1431.0 27 1815.5 28 2192.0 29 2776.0 30 3575.0 31 4745.0 32 7232.5 33 11426.5 34 17678.0 35 28091.0 36 42254.0 37 61210.0 38 89166.0 39 112947.0 40 125318.5 41 128134.0 42 150493.5 43 189654.5 44 190274.5 45 173638.5 46 179431.5 47 182315.0 48 165669.0 49 142969.0 50 121370.0 51 110032.0 52 104934.5 53 103921.5 54 94294.0 55 65484.5 56 43617.5 57 36139.5 58 32345.5 59 26999.5 60 20520.5 61 15331.0 62 10917.5 63 7726.0 64 5684.0 65 4121.0 66 2985.5 67 2295.5 68 1794.0 69 1368.0 70 1039.5 71 803.5 72 602.5 73 462.0 74 347.5 75 262.5 76 214.0 77 163.0 78 128.0 79 112.0 80 94.5 81 72.5 82 62.0 83 50.5 84 44.0 85 41.5 86 34.5 87 24.5 88 20.0 89 16.5 90 15.0 91 11.5 92 6.5 93 4.0 94 2.5 95 3.5 96 4.0 97 4.0 98 2.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25692357855838105 2 0.24402130277924838 3 0.2082595601305654 4 0.211239705351289 5 0.2244224653864898 6 0.22761297379926443 7 0.20485868852573969 8 0.20079166681275223 9 0.18115776888798513 10-11 0.16993839864526103 12-13 0.1973732649419222 14-15 0.19434052892318587 16-17 0.19546246594745828 18-19 0.18781926996960252 20-21 0.1893969939099856 22-23 0.15486236988160057 24-25 0.4595909838335887 26-27 0.3191910834054996 28-29 0.2734370891343905 30-31 0.27592638690699495 32-33 0.3186301148933634 34-35 0.31452803264836743 36-37 0.3070250787985457 38-39 0.3308487102983301 40-41 0.33619544142962826 42-43 0.3718695327482899 44-45 0.33768551403999003 46-47 0.36338488400222985 48-49 0.3405955381966966 50-51 0.36133384287973186 52-53 0.3509559254052121 54-55 0.32736018736348305 56-57 0.36270120362806385 58-59 0.35072803194715674 60-61 0.3178938437211846 62-63 0.2960511322798812 64-65 0.30227437671139223 66-67 0.3111622215755502 68-69 0.3174731173370825 70-71 0.2944208175414854 72-73 0.3015205752732092 74-75 0.299522124948724 76-77 0.30262498203147736 78-79 0.3048162652820094 80-81 0.3208389284098997 82-83 0.3386496786702241 84-85 0.332759509292794 86-87 0.3374576205819347 88-89 0.3326543276967685 90-91 0.30735815385262655 92-93 0.2916510355128129 94-95 0.30024086585489845 96-97 0.288583238962068 98-99 0.3066218826804478 100-101 0.3297092430080534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2852210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.77146224891669 #Duplication Level Percentage of deduplicated Percentage of total 1 85.47460789207398 33.99450141020577 2 6.140095701846238 4.884011688214266 3 2.282839966278938 2.723756506175431 4 1.226905263874501 1.9518326554072756 5 0.8298928291894363 1.650302566337717 6 0.6574477649508 1.5688595378625239 7 0.40915268573055996 1.139082041620309 8 0.3353699278109865 1.0670521938685256 9 0.2542619049457249 0.9101130978497891 >10 1.9351401326782498 15.273312259290853 >50 0.23820939943067573 6.6837656752099335 >100 0.19261991332914008 15.281533500975778 >500 0.01583450733898821 4.2008785962145705 >1k 0.006822421697505827 4.6384331155680245 >5k 4.442715691711942E-4 1.4214622549644602 >10k+ 3.5541725517239305E-4 2.611102900234872 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 21891 0.7675101061983515 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 20704 0.7258932547042468 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19889 0.6973189211173091 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11404 0.39983030702507877 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9933 0.34825626444055663 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 9838 0.34492551389974796 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7819 0.2741382997745608 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6761 0.23704425690955436 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5873 0.20591050448599507 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4578 0.16050711553497113 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4228 0.1482359293319917 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4089 0.14336251538280842 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3504 0.12285210415782849 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3202 0.11226382349125766 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3080 0.10798643858621909 No Hit TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA 2959 0.10374411421318908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006766682677642951 0.0 0.0 2 0.007432832785804692 0.0 0.0 3 0.008239225022000484 0.0 0.0 4 0.008695011938111149 0.0 0.0 5 0.01041297800652827 0.0 0.0 6 0.011429733434775139 0.0 0.0 7 0.013428183759260363 0.0 0.0 8 0.014935786635626409 0.0 0.0 9 0.016829055364086094 0.0 0.0 10-11 0.026085035814333447 0.0 0.0 12-13 0.03325491461007429 0.0 0.0 14-15 0.03981123409566617 0.0 0.0 16-17 0.045841645601130354 0.0 0.0 18-19 0.05218760189467115 0.0 0.0 20-21 0.05762198435599061 0.0 0.0 22-23 0.062320095645131315 0.0 0.0 24-25 0.06789472023448484 0.0 0.0 26-27 0.07404784360197882 0.0 0.0 28-29 0.0813229039937452 0.0 0.0 30-31 0.16369762394774579 0.0 0.0 32-33 0.17525006924455072 0.0 0.0 34-35 0.18490924581289597 0.0 0.0 36-37 0.19609355552361152 0.0 0.0 38-39 0.20848745358862075 0.0 0.0 40-41 0.2231252257021748 0.0 0.0 42-43 0.23881481377598424 0.0 0.0 44-45 0.2558367020661172 0.0 0.0 46-47 0.27431360243460334 0.0 0.0 48-49 0.295560284831762 0.0 0.0 50-51 0.31799902531721014 0.0 0.0 52-53 0.3421031410730626 0.0 0.0 54-55 0.3698009613597877 0.0 0.0 56-57 0.3996550043650362 0.0 0.0 58-59 0.43290991897511055 0.0 0.0 60-61 0.46742701273749127 0.0 0.0 62-63 0.5069051717790766 0.0 0.0 64-65 0.5481889482191002 0.0 0.0 66-67 0.5931891410520262 0.0 0.0 68-69 0.6414850238937526 0.0 0.0 70-71 0.6982655554815389 0.0 0.0 72-73 0.757903520428019 0.0 0.0 74-75 0.8222746571956483 0.0 0.0 76-77 0.8947447768572441 0.0 0.0 78-79 0.9763131045750488 0.0 0.0 80-81 1.0675931996592116 0.0 0.0 82-83 1.1717054494584902 0.0 0.0 84-85 1.293505737656063 0.0 0.0 86-87 1.427927817376701 0.0 0.0 88-89 1.5760410348466627 0.0 1.7530266004256348E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4130 0.0 89.81046 9 ATTCGGA 990 0.0 89.62187 2 AAGCGGC 1695 0.0 87.30822 9 CGGGAGA 5285 0.0 87.16684 4 GTTCGGA 1000 0.0 86.82779 2 AGAGCGG 4580 0.0 84.81812 8 ATCCGGA 1110 0.0 82.92516 2 ATCGGGA 7060 0.0 82.460785 2 TCGGAGA 1570 0.0 80.38204 4 TCGGGAG 5915 0.0 80.13006 3 ACCGGAA 540 0.0 79.95255 3 CGGAAAG 2875 0.0 79.86596 5 CGGAGAG 2315 0.0 79.10255 5 GGAAAGC 2220 0.0 78.42041 6 GGATCGG 2830 0.0 78.29848 1 TCGGGAA 1970 0.0 77.78951 3 TTGGGAG 3175 0.0 76.65815 3 GGAGAGC 5845 0.0 75.72044 6 TCGGAAA 2695 0.0 73.40986 4 GGTCCGG 505 0.0 73.28686 1 >>END_MODULE