FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_50330665-1.342000000356cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_50330665-1.342000000356cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852210
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA218910.7675101061983515No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT207040.7258932547042468Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC198890.6973189211173091No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114040.39983030702507877No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT99330.34825626444055663No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT98380.34492551389974796No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC78190.2741382997745608No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT67610.23704425690955436No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT58730.20591050448599507No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC45780.16050711553497113No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA42280.1482359293319917No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT40890.14336251538280842No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA35040.12285210415782849No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC32020.11226382349125766No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC30800.10798643858621909No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA29590.10374411421318908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC41300.089.810469
ATTCGGA9900.089.621872
AAGCGGC16950.087.308229
CGGGAGA52850.087.166844
GTTCGGA10000.086.827792
AGAGCGG45800.084.818128
ATCCGGA11100.082.925162
ATCGGGA70600.082.4607852
TCGGAGA15700.080.382044
TCGGGAG59150.080.130063
ACCGGAA5400.079.952553
CGGAAAG28750.079.865965
CGGAGAG23150.079.102555
GGAAAGC22200.078.420416
GGATCGG28300.078.298481
TCGGGAA19700.077.789513
TTGGGAG31750.076.658153
GGAGAGC58450.075.720446
TCGGAAA26950.073.409864
GGTCCGG5050.073.286861
Produced by FastQC (version 0.11.2)