FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410268
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1443434.232599901239433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1148303.367184045359485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG1900.054.9681741
CGCGTTG903.6379788E-1252.7309576
TGCGACC554.9858212E-543.1517522
AACGGGC1006.4028427E-1042.7252581
CGGACGG756.9538473E-637.978011
TGTACCG500.001621800837.9735452
CGTGCGG905.7282523E-736.9230651
AGCGGCC2100.036.1695331
CGCAGCC1950.034.082831
CGCGCGG600.00396324531.6483441
GTCACGG600.00396324531.6483441
CGCGTGG753.0901813E-431.648341
GGACCGC1051.913495E-631.6446192
GCGCTAG600.00397123831.6353197
ACGGTGC600.00397152431.6348538
CGGGGTG8150.030.8716961
CGGCCAC2800.030.5122133
CCGGCCG953.5094774E-529.9791152
GTTTGAT145650.029.5567053
GTGTTTG498150.029.4660281
Produced by FastQC (version 0.11.2)