##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3410268 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21316154624798 33.0 33.0 33.0 33.0 33.0 2 32.248634124942676 33.0 33.0 33.0 33.0 33.0 3 32.239146014330835 33.0 33.0 33.0 33.0 33.0 4 36.04048772706427 37.0 37.0 37.0 33.0 37.0 5 35.99620733619762 37.0 37.0 37.0 33.0 37.0 6 35.936435200987134 37.0 37.0 37.0 33.0 37.0 7 35.95585596205342 37.0 37.0 37.0 33.0 37.0 8 35.89480738757189 37.0 37.0 37.0 33.0 37.0 9 35.84839607913513 37.0 37.0 37.0 33.0 37.0 10-11 35.75173388132545 37.0 37.0 37.0 33.0 37.0 12-13 35.78110796570827 37.0 37.0 37.0 33.0 37.0 14-15 37.59143474940972 40.0 37.0 40.0 33.0 40.0 16-17 37.417105928331736 40.0 37.0 40.0 33.0 40.0 18-19 37.17842571316976 40.0 37.0 40.0 33.0 40.0 20-21 37.0928535528586 40.0 37.0 40.0 33.0 40.0 22-23 36.96692767254656 40.0 37.0 40.0 33.0 40.0 24-25 36.78283994102516 40.0 37.0 40.0 33.0 40.0 26-27 36.67098788130434 40.0 37.0 40.0 33.0 40.0 28-29 36.56904208115022 37.0 37.0 40.0 33.0 40.0 30-31 36.575476912664925 38.5 37.0 40.0 33.0 40.0 32-33 36.44128364691572 37.0 37.0 40.0 33.0 40.0 34-35 36.35224665627452 37.0 37.0 40.0 33.0 40.0 36-37 36.239749779196245 37.0 37.0 40.0 33.0 40.0 38-39 36.159925700854004 37.0 37.0 40.0 33.0 40.0 40-41 36.06724310230164 37.0 37.0 40.0 33.0 40.0 42-43 35.9189021214755 37.0 37.0 40.0 30.0 40.0 44-45 35.79158661430715 37.0 37.0 40.0 27.0 40.0 46-47 35.67817690574465 37.0 37.0 40.0 27.0 40.0 48-49 35.523989463584684 37.0 37.0 40.0 27.0 40.0 50-51 34.26705349843473 37.0 35.0 38.5 24.5 38.5 52-53 34.71418272698803 37.0 35.0 38.5 27.0 40.0 54-55 35.438579753849254 37.0 37.0 40.0 27.0 40.0 56-57 35.42343592937564 37.0 37.0 40.0 27.0 40.0 58-59 35.25088072257078 37.0 37.0 40.0 27.0 40.0 60-61 34.96040296539744 37.0 35.0 40.0 27.0 40.0 62-63 34.712619506736715 37.0 33.0 40.0 27.0 40.0 64-65 34.43471964666706 37.0 33.0 38.5 27.0 40.0 66-67 34.166603328536056 37.0 33.0 37.0 27.0 40.0 68-69 33.89202520153842 37.0 33.0 37.0 27.0 40.0 70-71 33.577534082365375 37.0 33.0 37.0 22.0 40.0 72-73 33.2802715211825 37.0 33.0 37.0 22.0 38.5 74-75 32.99364727346942 37.0 33.0 37.0 22.0 37.0 76-77 32.73335072199605 37.0 33.0 37.0 22.0 37.0 78-79 32.46698969113278 37.0 33.0 37.0 22.0 37.0 80-81 32.213228403163626 37.0 33.0 37.0 22.0 37.0 82-83 31.957026397925326 37.0 33.0 37.0 15.0 37.0 84-85 31.774826347958577 37.0 33.0 37.0 15.0 37.0 86-87 31.56709326070561 33.0 33.0 37.0 15.0 37.0 88-89 31.30031114856662 33.0 33.0 37.0 15.0 37.0 90-91 31.175749677151472 33.0 33.0 37.0 15.0 37.0 92-93 30.975624203141805 33.0 33.0 37.0 15.0 37.0 94-95 30.786001715994168 33.0 33.0 37.0 15.0 37.0 96-97 30.58607959843625 33.0 27.0 37.0 15.0 37.0 98-99 30.388392935687165 33.0 27.0 37.0 15.0 37.0 100-101 29.167182901754348 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.22987305737547103 2306 2 0.20977304954173803 2306 3 0.1739355902975177 2306 4 0.21375556440993648 2306 5 0.3178798198102726 2306 6 0.23773495283801083 2306 7 0.21135765791131433 2306 8 0.2007592228891042 2306 9 0.15874164585125783 2306 10-11 0.1835842369494074 2306 12-13 0.18551684465798246 2306 14-15 0.14310093024965198 2306 16-17 0.26293150060394055 2306 18-19 0.44262445851379795 2306 20-21 0.30099881594501454 2306 22-23 0.36750623945513894 2306 24-25 0.3947338085808454 2306 26-27 0.40077385850830893 2306 28-29 0.30516324777931203 2306 30-31 0.17975696304789324 2306 32-33 0.24195452970051434 2306 34-35 0.183319782667283 2306 36-37 0.2989153386424661 2306 38-39 0.06650521253038022 2306 40-41 0.31083360871196675 2306 42-43 0.1758714203832099 2306 44-45 0.18458028536810644 2306 46-47 0.12994396300666722 2306 48-49 0.21492043450984966 2306 50-51 0.31027411732092247 2306 52-53 0.23919865923276262 2306 54-55 0.2019928945076117 2306 56-57 0.19707221909555983 2306 58-59 0.24355134319876726 2306 60-61 0.14433028234613943 2306 62-63 0.07813064778616763 2306 64-65 0.10457239752356173 2306 66-67 0.21697480270301384 2306 68-69 0.249546389788172 2306 70-71 0.23648710867559686 2306 72-73 0.19100817061250552 2306 74-75 0.22934660051758726 2306 76-77 0.22708134142730074 2306 78-79 0.2424774352206498 2306 80-81 0.21723687667586233 2306 82-83 0.35009687031630676 2306 84-85 0.3374725877876976 2306 86-87 0.3030700374099844 2306 88-89 0.4614622666239079 2306 90-91 0.2671360894581838 2306 92-93 0.475079584055603 2306 94-95 0.38377008782304856 2306 96-97 0.42975820824077715 2306 98-99 0.3922330464862469 2306 100-101 0.3724893470877433 2307 1 0.21230972627306244 2307 2 0.20663196712097687 2307 3 0.21826189900986748 2307 4 0.16438705867788173 2307 5 0.3617492715259729 2307 6 0.3911514961431237 2307 7 0.3165811239974303 2307 8 0.27339694443806906 2307 9 0.2860939639050386 2307 10-11 0.2832463965363132 2307 12-13 0.12552044259739858 2307 14-15 0.24397586983933195 2307 16-17 0.2828840565892534 2307 18-19 0.40380304792844157 2307 20-21 0.20136765555365344 2307 22-23 0.3647752611459296 2307 24-25 0.3793302574959512 2307 26-27 0.40129525481371786 2307 28-29 0.28927285686928883 2307 30-31 0.2908977181984653 2307 32-33 0.29944177556428286 2307 34-35 0.34013404107759726 2307 36-37 0.3387858941328048 2307 38-39 0.12593549813460214 2307 40-41 0.2055433164911875 2307 42-43 0.1992145769042466 2307 44-45 0.19961311656528835 2307 46-47 0.051423254781767014 2307 48-49 0.04169159387468824 2307 50-51 0.2337177925448941 2307 52-53 0.25091279786421694 2307 54-55 0.1131466591566408 2307 56-57 0.23938078508801652 2307 58-59 0.2782630735484517 2307 60-61 0.3448905038735077 2307 62-63 0.2717678850362262 2307 64-65 0.25211738828223673 2307 66-67 0.3055537335153531 2307 68-69 0.2561156783261396 2307 70-71 0.13820187237278958 2307 72-73 0.1573305937535494 2307 74-75 0.46145053624660193 2307 76-77 0.3905967620025379 2307 78-79 0.4928337989235345 2307 80-81 0.2853192410302441 2307 82-83 0.4251784464780286 2307 84-85 0.49500745044855066 2307 86-87 0.5356097405190106 2307 88-89 0.7087692750338412 2307 90-91 0.7232054016266893 2307 92-93 0.8080546479977961 2307 94-95 0.5626598389299993 2307 96-97 0.7214024513090287 2307 98-99 0.9170738198805992 2307 100-101 0.6557996342978321 2201 1 0.3423764669003191 2201 2 0.332672658252271 2201 3 0.33466017838559026 2201 4 0.4776461752810732 2201 5 0.4768116177415749 2201 6 0.4692601221570811 2201 7 0.39063072241704333 2201 8 0.3724761190891144 2201 9 0.4602677905010779 2201 10-11 0.46749351135292017 2201 12-13 0.42310311596855144 2201 14-15 0.5235578644379686 2201 16-17 0.5410557412097745 2201 18-19 0.6298968882279965 2201 20-21 0.6516422862709916 2201 22-23 0.6436274152080799 2201 24-25 0.6042029476282949 2201 26-27 0.7378415514212904 2201 28-29 0.7140036291170944 2201 30-31 0.61732414101931 2201 32-33 0.6944836945694277 2201 34-35 0.7184684710127414 2201 36-37 0.6357209320816324 2201 38-39 0.7294698377802007 2201 40-41 0.6114103763336303 2201 42-43 0.7112249281850254 2201 44-45 0.672746051920214 2201 46-47 0.6466455928008727 2201 48-49 0.7733167097382676 2201 50-51 0.8507705110768171 2201 52-53 0.7442315311850578 2201 54-55 0.8559443065055063 2201 56-57 0.9116380398694446 2201 58-59 0.8850951429881562 2201 60-61 0.8775443443011355 2201 62-63 0.8891651926786608 2201 64-65 0.8718173196045527 2201 66-67 0.8107494649327478 2201 68-69 0.9372808617788166 2201 70-71 0.8574377089999388 2201 72-73 0.8506395617151767 2201 74-75 0.8663461029335977 2201 76-77 0.8642888041952901 2201 78-79 0.9629221033850328 2201 80-81 1.050034356685714 2201 82-83 0.9746094942370291 2201 84-85 0.9972655697044068 2201 86-87 1.1061081100241843 2201 88-89 1.341717356941185 2201 90-91 1.2551156339621947 2201 92-93 1.1430183961027574 2201 94-95 1.2083994208125368 2201 96-97 1.1617749277240854 2201 98-99 1.2744395421890005 2201 100-101 1.2359612269798959 1101 1 0.22839901118373263 1101 2 0.19994684943949892 1101 3 0.2354667952791587 1101 4 0.3035311116007904 1101 5 0.376174814930863 1101 6 0.37732043543478255 1101 7 0.295760278850949 1101 8 0.2829284686719049 1101 9 0.3457442946732314 1101 10-11 0.26828914888768907 1101 12-13 0.34256805521609834 1101 14-15 0.3872491248683616 1101 16-17 0.4017715954038863 1101 18-19 0.47903391062587986 1101 20-21 0.48750306800133814 1101 22-23 0.5076414687873552 1101 24-25 0.6253857905502684 1101 26-27 0.6256683703350632 1101 28-29 0.6629547343725548 1101 30-31 0.5765072767241861 1101 32-33 0.6229290173625728 1101 34-35 0.5980058740284022 1101 36-37 0.6121541043843663 1101 38-39 0.6233433555667602 1101 40-41 0.49662337589445826 1101 42-43 0.6013105671837096 1101 44-45 0.757834618747907 1101 46-47 0.5780370435401494 1101 48-49 0.5153932724670085 1101 50-51 0.644365153149721 1101 52-53 0.760067426368785 1101 54-55 0.6537703934620254 1101 56-57 0.7215289782386449 1101 58-59 0.6365561296665376 1101 60-61 0.8415078928299025 1101 62-63 0.8101862730475631 1101 64-65 0.6686852745159797 1101 66-67 0.6555964858667309 1101 68-69 0.7160936271133167 1101 70-71 0.7098685390394621 1101 72-73 0.7757098296115288 1101 74-75 0.9027961102531634 1101 76-77 0.8980730231968934 1101 78-79 0.6958374891265038 1101 80-81 0.8767215181554846 1101 82-83 0.969680494090639 1101 84-85 1.0550967803617048 1101 86-87 0.9873787878166134 1101 88-89 1.225207329126814 1101 90-91 1.0508058696524323 1101 92-93 1.1668728830007176 1101 94-95 1.2168315683751239 1101 96-97 1.1617478453056975 1101 98-99 1.076416558731239 1101 100-101 0.9588253757136087 2304 1 0.23023030929359578 2304 2 0.2540777485649599 2304 3 0.2632171680676194 2304 4 0.31502669859148114 2304 5 0.35694497573366846 2304 6 0.2982497869843712 2304 7 0.3316277676672925 2304 8 0.2590135853446114 2304 9 0.3062097764771252 2304 10-11 0.2662275279631743 2304 12-13 0.2855699090830157 2304 14-15 0.35963154537373754 2304 16-17 0.45438917489850894 2304 18-19 0.49300380543908773 2304 20-21 0.5045548150694046 2304 22-23 0.6203086190714799 2304 24-25 0.6794381018387057 2304 26-27 0.6530276175038878 2304 28-29 0.5079551373870999 2304 30-31 0.5059559528213953 2304 32-33 0.45061490782322267 2304 34-35 0.5882928203871671 2304 36-37 0.7088052549909136 2304 38-39 0.5069849950299457 2304 40-41 0.4673536382569239 2304 42-43 0.5029881152431912 2304 44-45 0.5780006607420276 2304 46-47 0.4999971047983607 2304 48-49 0.5515789412696535 2304 50-51 0.6604158120785115 2304 52-53 0.6058529017244396 2304 54-55 0.6608795701033046 2304 56-57 0.5978158036930452 2304 58-59 0.6136693364533059 2304 60-61 0.4981896725421464 2304 62-63 0.43339447036348844 2304 64-65 0.4965007341540755 2304 66-67 0.5870477412570452 2304 68-69 0.6309362499320059 2304 70-71 0.585245760657159 2304 72-73 0.6152389071112268 2304 74-75 0.6456559014916365 2304 76-77 0.8349855287383505 2304 78-79 0.6980707942488138 2304 80-81 0.7416451468984846 2304 82-83 0.8199347962709567 2304 84-85 0.8648342163831408 2304 86-87 0.7681678903032072 2304 88-89 0.7819795856052743 2304 90-91 0.8348976763666549 2304 92-93 0.8761019161688175 2304 94-95 0.90165202539362 2304 96-97 0.9880084748144249 2304 98-99 0.7806637275540318 2304 100-101 0.7230677083618176 2202 1 0.21657997695963616 2202 2 0.1594639614882567 2202 3 0.17164588576204665 2202 4 0.23746922693131012 2202 5 0.279761312821158 2202 6 0.29413712554261195 2202 7 0.242398816122396 2202 8 0.11443339131606223 2202 9 0.2643940837027685 2202 10-11 0.2607973532946275 2202 12-13 0.2507513651082647 2202 14-15 0.2468834427305282 2202 16-17 0.26527370510852677 2202 18-19 0.36401281357947113 2202 20-21 0.4622710004907944 2202 22-23 0.40292276273953576 2202 24-25 0.37424889416021045 2202 26-27 0.5235070951358125 2202 28-29 0.5108426856149322 2202 30-31 0.534165504572286 2202 32-33 0.4828305644793147 2202 34-35 0.39967722892760804 2202 36-37 0.28170637817715516 2202 38-39 0.38895516991275514 2202 40-41 0.3434025601419961 2202 42-43 0.30317413961690676 2202 44-45 0.35873944762742127 2202 46-47 0.2689746900474148 2202 48-49 0.33735764189972883 2202 50-51 0.43262816299501594 2202 52-53 0.2992610503725359 2202 54-55 0.5608379040105405 2202 56-57 0.4907530757538865 2202 58-59 0.4612795239468923 2202 60-61 0.46600156371598445 2202 62-63 0.51659274280847 2202 64-65 0.4767537227110168 2202 66-67 0.6463255060261304 2202 68-69 0.674410551632775 2202 70-71 0.5887996220211704 2202 72-73 0.4111458352374058 2202 74-75 0.65802583364745 2202 76-77 0.6264532388555963 2202 78-79 0.6220634619347507 2202 80-81 0.7285616494259912 2202 82-83 0.7072027631951912 2202 84-85 0.6840184868783403 2202 86-87 0.7834500489511562 2202 88-89 0.8943540708052033 2202 90-91 0.8054800374911544 2202 92-93 0.727046347604567 2202 94-95 0.9167612448870734 2202 96-97 0.9599569404768182 2202 98-99 0.7949514860636313 2202 100-101 0.9681760555073282 1102 1 0.050334742157723156 1102 2 0.13000736497054532 1102 3 0.1392934450934007 1102 4 0.08147434956470789 1102 5 0.1675513517572469 1102 6 0.12238382353850596 1102 7 0.12344958139030382 1102 8 0.102944555078885 1102 9 0.1594786391521481 1102 10-11 0.2383027457316942 1102 12-13 0.13720074892324874 1102 14-15 0.2837187330494473 1102 16-17 0.23057775419970028 1102 18-19 0.38247927429358697 1102 20-21 0.4129603815713736 1102 22-23 0.45655678534485844 1102 24-25 0.42021132963640895 1102 26-27 0.5029116580902411 1102 28-29 0.5052709854737074 1102 30-31 0.43037490857527416 1102 32-33 0.46970254373279374 1102 34-35 0.5224024422615869 1102 36-37 0.5274157000027415 1102 38-39 0.45274700947920365 1102 40-41 0.42942011265189706 1102 42-43 0.45119853684933986 1102 44-45 0.5631331748013793 1102 46-47 0.5402468179474411 1102 48-49 0.4383269351886696 1102 50-51 0.4329110568610872 1102 52-53 0.5448992851148091 1102 54-55 0.5740737371366151 1102 56-57 0.5667878927891792 1102 58-59 0.6222212259934778 1102 60-61 0.6217669988852847 1102 62-63 0.6248036463671127 1102 64-65 0.7832781352153617 1102 66-67 0.8183487935001139 1102 68-69 0.7537270102379168 1102 70-71 0.8477847748202265 1102 72-73 0.7733870673456451 1102 74-75 0.9077729381669144 1102 76-77 0.8679389698053441 1102 78-79 0.7480070475163316 1102 80-81 0.9117879703367251 1102 82-83 0.8797682033096876 1102 84-85 0.9851551893394479 1102 86-87 0.9464143694168179 1102 88-89 1.1535335767808839 1102 90-91 1.0382358746315568 1102 92-93 1.1301537440065026 1102 94-95 1.2182621095678137 1102 96-97 1.2758833924350377 1102 98-99 1.3685995224602223 1102 100-101 1.195850347945406 2305 1 0.2969300306214322 2305 2 0.2701566687889638 2305 3 0.24056104240517584 2305 4 0.31125373826451863 2305 5 0.2542945296157626 2305 6 0.3577082090455903 2305 7 0.3231181728137429 2305 8 0.30651843852262317 2305 9 0.29244552270470336 2305 10-11 0.3699401281263661 2305 12-13 0.2915078056879423 2305 14-15 0.40873416053275236 2305 16-17 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19659.0 21 21645.0 22 24082.0 23 25871.0 24 28169.0 25 31626.0 26 35466.0 27 40342.0 28 47049.0 29 55696.0 30 66490.0 31 82552.0 32 105982.0 33 141745.0 34 206010.0 35 334534.0 36 654171.0 37 1014103.0 38 340546.0 39 188.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.90102944989965 32.52769436034098 19.22960901837392 15.341667171385446 2 30.35977339359003 26.201197118238134 30.388847356988585 13.050182131183252 3 34.02021017714393 24.144952348543423 24.6970339462447 17.137803528067945 4 29.384591829567793 24.114656296477843 29.233247257944928 17.267504616009436 5 29.21555741180855 22.563437707395614 30.369007681242827 17.85199719955301 6 26.819408830275904 23.181561129197426 31.91748642938519 18.081543611141484 7 30.880044431253506 23.12405781940106 25.93398197478115 20.06191577456428 8 27.238438225246348 28.473557176666116 25.044914217810888 19.243090380276648 9 25.1993425024568 26.362302299037992 28.909003573931013 19.52935162457419 10-11 20.207254038213655 31.518232621316788 32.935044303730535 15.33946903673902 12-13 20.40195764719157 30.813770524751348 32.90756622529691 15.876705602760168 14-15 20.479945983158522 30.761318958224805 33.048778230366665 15.70995682825001 16-17 20.097983690344858 30.842237502392884 33.06129027187117 15.998488535391086 18-19 19.43261177775091 31.238244967711353 32.67500500573585 16.654138248801893 20-21 19.453367679647897 31.5138453989821 32.14014412818315 16.892642793186855 22-23 19.500309936425218 30.94096581212522 32.25913181674731 17.299592434702245 24-25 19.375287074735333 30.72736009682539 32.398691160175254 17.498661668264024 26-27 19.432197464452212 31.123885139076187 32.20268671387493 17.241230682596672 28-29 19.20695802606996 31.347766203902776 32.02826242368674 17.41701334634053 30-31 18.6925570168262 32.40946764595665 31.819911906241494 17.078063430975654 32-33 18.431118772098056 32.875468434707415 31.864884898900463 16.82852789429407 34-35 18.804868625613775 32.31996531528544 32.316023870448824 16.55914218865196 36-37 18.80916656041631 31.913122647326603 32.744154869935585 16.5335559223215 38-39 19.104108294608217 31.730256662504868 32.139768155303706 17.02586688758321 40-41 19.013652009172635 31.686938910542985 31.580717226579928 17.718691853704456 42-43 19.288477861375043 31.27958556371646 31.522524171721937 17.909412403186558 44-45 18.936543615966485 31.191660757840108 31.79774454451038 18.07405108168303 46-47 19.05385594359475 31.474185483352397 31.68938677270609 17.782571800346762 48-49 19.00562754629037 32.03795614768315 31.134112251409828 17.82230405461665 50-51 19.538626521029258 31.920719899894877 30.29979446902733 18.240859110048536 52-53 19.70516601680604 31.774153349452583 30.34297398703692 18.177706646704454 54-55 20.05325565376147 31.79857930990051 30.30565297453404 17.842512061803976 56-57 20.206154105130324 31.831299190816225 30.12577722372507 17.836769480328382 58-59 19.985943544366037 32.44016332795194 29.981725430664703 17.59216769701732 60-61 19.839593058425784 32.30862069650181 29.827831392690353 18.023954852382047 62-63 19.651308105574223 32.17498734338031 30.21980243762893 17.953902113416547 64-65 19.569445753608893 31.74836122429563 30.62500744446868 18.057185577626804 66-67 19.987746583102638 31.427367180137583 30.283602186908432 18.301284049851347 68-69 20.36388114790505 31.464466678079596 30.40485884740035 17.766793326615 70-71 20.06133637843931 31.717556814154918 30.506033209339172 17.715073598066596 72-73 20.164047846798486 31.696879105883358 30.139777411952583 17.999295635365577 74-75 20.51647143436299 31.30954038736093 29.79386462615934 18.38012355211674 76-77 20.688063977827042 31.04268364773844 29.351507479902523 18.917744894531992 78-79 20.50549645113692 31.23987714489826 29.58339791471023 18.67122848925459 80-81 20.34504374305599 31.589219743663133 29.204788571062135 18.860947942218747 82-83 20.02105081985662 31.666832160533463 29.112695439062584 19.199421580547334 84-85 20.113047338609817 31.722728940277552 29.600656878409538 18.563566842703096 86-87 20.498941152311076 31.329785011847676 29.927069254346456 18.24420458149479 88-89 20.987943066490974 31.234777323444902 29.73372837640768 18.043551233656448 90-91 20.967875237426888 31.221202155062628 29.297331112937258 18.513591494573227 92-93 20.75078531506411 31.363669246056368 29.18671001656793 18.698835422311593 94-95 20.671986156367 31.136748666684216 29.244336505097323 18.94692867185146 96-97 20.804882248125466 31.554602926237703 28.99011996689453 18.6503948587423 98-99 21.421590297122247 31.283513054925894 28.6787632048532 18.616133443098654 100-101 21.853314338709833 31.207553525343773 28.10074112863072 18.838391007315675 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 138445.0 1 78773.5 2 13792.5 3 8306.5 4 7350.5 5 6355.0 6 6524.0 7 7338.0 8 7870.5 9 7705.5 10 7236.5 11 6827.0 12 6437.5 13 6155.5 14 5961.5 15 5860.5 16 5896.0 17 5977.5 18 5940.0 19 5947.5 20 6173.0 21 6543.5 22 7026.5 23 7780.0 24 8931.5 25 10432.5 26 12609.0 27 15712.5 28 19449.5 29 23260.0 30 27794.0 31 33793.5 32 42486.0 33 57391.0 34 77607.0 35 98852.0 36 127799.0 37 165937.5 38 194893.0 39 199573.5 40 198133.5 41 197763.0 42 190486.0 43 184921.0 44 174181.0 45 160551.0 46 155919.0 47 144637.5 48 120120.5 49 96730.0 50 77356.5 51 62097.0 52 50410.0 53 39308.5 54 31323.0 55 26275.0 56 20833.5 57 15389.5 58 11159.0 59 8063.0 60 5783.5 61 4390.5 62 3402.5 63 2631.0 64 2130.5 65 1591.0 66 1118.0 67 825.5 68 616.0 69 506.0 70 409.5 71 318.0 72 251.5 73 196.0 74 161.0 75 144.0 76 118.5 77 98.5 78 81.0 79 59.0 80 41.5 81 38.5 82 32.5 83 29.0 84 29.0 85 19.0 86 13.0 87 12.5 88 8.5 89 8.0 90 8.0 91 4.5 92 4.0 93 1.5 94 0.0 95 2.5 96 2.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26490586663570137 2 0.2521502708878012 3 0.2149097959456559 4 0.21807670247616903 5 0.2332368013305699 6 0.2364623542783148 7 0.21282784813392966 8 0.20860530609324546 9 0.1892226651981604 10-11 0.1771121800398092 12-13 0.20523313710242128 14-15 0.20189029132021297 16-17 0.20369366865008848 18-19 0.19565910948934218 20-21 0.19648015933058632 22-23 0.1623772677103383 24-25 0.4726021532618551 26-27 0.33303834185465775 28-29 0.26606413337602797 30-31 0.27072652354595006 32-33 0.31223352534170334 34-35 0.30787902886224777 36-37 0.301427922966758 38-39 0.32537032280160977 40-41 0.3297248192810653 42-43 0.36642281486381717 44-45 0.33422593180360016 46-47 0.360279602658794 48-49 0.3357800618602409 50-51 0.35891607345815635 52-53 0.3473920524721224 54-55 0.3226872492132583 56-57 0.3599717089683274 58-59 0.34687889632134483 60-61 0.31271735828386504 62-63 0.2895227002687179 64-65 0.2959444829555918 66-67 0.30496136960496945 68-69 0.311236536248764 70-71 0.28851104957147067 72-73 0.2944196761075669 74-75 0.29330539418016416 76-77 0.29587117493405213 78-79 0.2980704155802418 80-81 0.3154297550808324 82-83 0.33283307939434675 84-85 0.32727633136164075 86-87 0.33105902527308706 88-89 0.32680716002378696 90-91 0.3012666453193708 92-93 0.2850802341634147 94-95 0.2933640405973959 96-97 0.28213325169752057 98-99 0.30164784703137704 100-101 0.3243293488957466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3410268.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.960165897058836 #Duplication Level Percentage of deduplicated Percentage of total 1 82.00159515197944 36.86805321856451 2 8.789317225842362 7.903383211914991 3 3.014323498632888 4.065734536978124 4 1.6628243880723579 2.990434413816342 5 0.9236186778609243 2.076302449112465 6 0.6489793870788599 1.7506932544102263 7 0.44558126792372427 1.4023385408530726 8 0.3141339114343692 1.1298810217584982 9 0.24972685931269697 1.0104984921285298 >10 1.6601868349558557 13.945362283273141 >50 0.16107755571370105 5.042246806247156 >100 0.1171971938848505 10.721014991544422 >500 0.009366594814876339 2.817508691397506 >1k 0.0019481262426129427 1.0912764209904313 >5k 0.0 0.0 >10k+ 1.2332625048258803E-4 7.185271667010613 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144343 4.232599901239433 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114830 3.367184045359485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.225552947744869E-4 0.0 0.0 2 3.518785033903494E-4 0.0 0.0 3 4.105249206220743E-4 0.0 0.0 4 4.398481292379367E-4 0.0 0.0 5 4.691713378537992E-4 0.0 0.0 6 5.571409637013865E-4 0.0 0.0 7 7.037570067806988E-4 0.0 0.0 8 8.796962584758734E-4 0.0 0.0 9 0.0012608979704820853 0.0 0.0 10-11 0.002536457545272102 0.0 0.0 12-13 0.00801989755643838 0.0 0.0 14-15 0.016567612867962284 0.0 0.0 16-17 0.029601779097713145 0.0 0.0 18-19 0.04051001270281397 0.0 0.0 20-21 0.046975780202611644 0.0 0.0 22-23 0.05396936545749484 0.0 0.0 24-25 0.06150543007177149 0.0 0.0 26-27 0.07134336656239333 0.0 0.0 28-29 0.08291137236135107 0.0 0.0 30-31 0.09584290736094642 0.0 0.0 32-33 0.11024060279133488 0.0 0.0 34-35 0.12437438934418057 0.0 0.0 36-37 0.1401502755795146 0.0 0.0 38-39 0.15868254342473964 0.0 0.0 40-41 0.17871029490937368 0.0 0.0 42-43 0.20005759078172156 0.0 0.0 44-45 0.22180074997038357 0.0 0.0 46-47 0.24625630595601286 0.0 0.0 48-49 0.27296974900506354 0.0 0.0 50-51 0.30224897280800217 0.0 0.0 52-53 0.3313229341506298 0.0 0.0 54-55 0.36412094298747194 0.0 0.0 56-57 0.39754940080955514 0.0 0.0 58-59 0.4342913812052308 0.0 0.0 60-61 0.47123862406121747 0.0 0.0 62-63 0.50949074970061 1.4661604307931225E-5 0.0 64-65 0.5535342090416355 2.932320861586245E-5 0.0 66-67 0.5987799199359112 2.932320861586245E-5 0.0 68-69 0.6501395198265942 2.932320861586245E-5 0.0 70-71 0.7063081259302788 2.932320861586245E-5 0.0 72-73 0.7681947577140564 2.932320861586245E-5 0.0 74-75 0.8356234759262322 2.932320861586245E-5 0.0 76-77 0.9135205796142708 2.932320861586245E-5 0.0 78-79 0.9985285613916561 2.932320861586245E-5 0.0 80-81 1.097025219132338 2.932320861586245E-5 0.0 82-83 1.2112097934825063 2.932320861586245E-5 0.0 84-85 1.3427830305418813 2.932320861586245E-5 0.0 86-87 1.4909385420735262 2.932320861586245E-5 0.0 88-89 1.6606026271249061 2.932320861586245E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAG 190 0.0 54.968174 1 CGCGTTG 90 3.6379788E-12 52.730957 6 TGCGACC 55 4.9858212E-5 43.151752 2 AACGGGC 100 6.4028427E-10 42.725258 1 CGGACGG 75 6.9538473E-6 37.97801 1 TGTACCG 50 0.0016218008 37.973545 2 CGTGCGG 90 5.7282523E-7 36.923065 1 AGCGGCC 210 0.0 36.169533 1 CGCAGCC 195 0.0 34.08283 1 CGCGCGG 60 0.003963245 31.648344 1 GTCACGG 60 0.003963245 31.648344 1 CGCGTGG 75 3.0901813E-4 31.64834 1 GGACCGC 105 1.913495E-6 31.644619 2 GCGCTAG 60 0.003971238 31.635319 7 ACGGTGC 60 0.003971524 31.634853 8 CGGGGTG 815 0.0 30.871696 1 CGGCCAC 280 0.0 30.512213 3 CCGGCCG 95 3.5094774E-5 29.979115 2 GTTTGAT 14565 0.0 29.556705 3 GTGTTTG 49815 0.0 29.466028 1 >>END_MODULE