FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6072226
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC207220.3412587080915631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108670.17896237722377265No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99660.1641243260708676No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG92140.1517400702806516No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA78170.12873368020228496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG3150.049.7178841
CGGAAAG3550.048.1125035
GTTACGG2850.046.6253851
CGACGGG3000.041.1302571
CGCGGAG5300.040.2945141
CGAGGTG2900.039.275571
CGTTTGA6550.039.1256681
ATGACCG1950.038.9398881
CGAGGGG11200.038.5596121
TCGGGAA3450.038.5074043
GAGCGGC6200.038.2507749
GTTGACG2650.037.6082151
CGAGTCG651.335791E-436.5061381
CCAGCCG4450.036.255242
GACGTCG1054.7184585E-836.142057
CGAGCGG1850.035.914151
GGACGGG8050.035.372411
TGTACCG958.7776425E-734.964422
GATCGGG17350.033.91811
CGGGACG702.0650707E-433.898561
Produced by FastQC (version 0.11.2)