##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3948268 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24298781136437 33.0 33.0 33.0 33.0 33.0 2 32.257708190021546 33.0 33.0 33.0 33.0 33.0 3 32.24044745696087 33.0 33.0 33.0 33.0 33.0 4 36.03232936568642 37.0 37.0 37.0 37.0 37.0 5 35.98987480079873 37.0 37.0 37.0 33.0 37.0 6 35.97094878057923 37.0 37.0 37.0 33.0 37.0 7 35.97468991466638 37.0 37.0 37.0 33.0 37.0 8 35.947755066272094 37.0 37.0 37.0 33.0 37.0 9 35.88550574581057 37.0 37.0 37.0 33.0 37.0 10-11 35.82565469213336 37.0 37.0 37.0 33.0 37.0 12-13 35.854144019605556 37.0 37.0 37.0 33.0 37.0 14-15 37.729656902722915 40.0 37.0 40.0 33.0 40.0 16-17 37.696342674813366 40.0 37.0 40.0 33.0 40.0 18-19 37.6730247288178 40.0 37.0 40.0 33.0 40.0 20-21 37.643854216583065 40.0 37.0 40.0 33.0 40.0 22-23 37.63251722020897 40.0 37.0 40.0 33.0 40.0 24-25 37.477223430628314 40.0 37.0 40.0 33.0 40.0 26-27 37.401823660399955 40.0 37.0 40.0 33.0 40.0 28-29 37.32828090696984 40.0 37.0 40.0 33.0 40.0 30-31 37.35532580361819 40.0 37.0 40.0 33.0 40.0 32-33 37.26199449986677 40.0 37.0 40.0 33.0 40.0 34-35 37.209886968159196 40.0 37.0 40.0 33.0 40.0 36-37 37.112509079930746 40.0 37.0 40.0 33.0 40.0 38-39 37.07810526033187 40.0 37.0 40.0 33.0 40.0 40-41 37.00477525841711 40.0 37.0 40.0 33.0 40.0 42-43 36.883361894380016 40.0 37.0 40.0 33.0 40.0 44-45 36.783452643032334 40.0 37.0 40.0 33.0 40.0 46-47 36.708834861260684 40.0 37.0 40.0 33.0 40.0 48-49 36.58166897485176 38.5 37.0 40.0 33.0 40.0 50-51 35.40697515974093 37.0 35.0 38.5 30.0 38.5 52-53 35.813296361847776 37.0 37.0 38.5 33.0 40.0 54-55 36.59073003656287 37.0 37.0 40.0 33.0 40.0 56-57 36.61853615306762 37.0 37.0 40.0 33.0 40.0 58-59 36.459600893353745 37.0 37.0 40.0 33.0 40.0 60-61 36.18750006331891 37.0 37.0 40.0 33.0 40.0 62-63 35.95119467574136 37.0 37.0 40.0 33.0 40.0 64-65 35.69478110401827 37.0 37.0 40.0 33.0 40.0 66-67 35.45305663141408 37.0 37.0 38.5 33.0 40.0 68-69 35.20624157225396 37.0 35.0 37.0 27.0 40.0 70-71 34.886429441973036 37.0 33.0 37.0 27.0 40.0 72-73 34.62659006430161 37.0 33.0 37.0 27.0 40.0 74-75 34.37736686060825 37.0 33.0 37.0 27.0 37.0 76-77 34.15036428631491 37.0 33.0 37.0 27.0 37.0 78-79 33.91130807229904 37.0 33.0 37.0 27.0 37.0 80-81 33.69504780323929 37.0 33.0 37.0 27.0 37.0 82-83 33.482535126794836 37.0 33.0 37.0 27.0 37.0 84-85 33.348011203900036 37.0 33.0 37.0 27.0 37.0 86-87 33.197857516257756 37.0 33.0 37.0 27.0 37.0 88-89 32.97972452731172 37.0 33.0 37.0 27.0 37.0 90-91 32.920808440561785 37.0 33.0 37.0 27.0 37.0 92-93 32.780453353217155 35.0 33.0 37.0 27.0 37.0 94-95 32.652052874830176 33.0 33.0 37.0 27.0 37.0 96-97 32.51055450136617 33.0 33.0 37.0 27.0 37.0 98-99 32.36982456611355 33.0 33.0 37.0 22.0 37.0 100-101 31.082931300509493 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.29578568584569354 2306 2 0.22286703987411727 2306 3 0.18993078034968391 2306 4 0.2317567267417715 2306 5 0.3297564964671267 2306 6 0.355422800111171 2306 7 0.31493523036910887 2306 8 0.23385374987135776 2306 9 0.34173800591335635 2306 10-11 0.30470471206535166 2306 12-13 0.3052401339583355 2306 14-15 0.5224048409163942 2306 16-17 0.44805927752947383 2306 18-19 0.47211316368939293 2306 20-21 0.49549430497762614 2306 22-23 0.47253215306298557 2306 24-25 0.4814157642937289 2306 26-27 0.515356762628393 2306 28-29 0.3991667992895245 2306 30-31 0.39925457891689575 2306 32-33 0.40742570514850485 2306 34-35 0.41280256151424766 2306 36-37 0.4409789158358066 2306 38-39 0.38370467453167834 2306 40-41 0.3766082824810226 2306 42-43 0.30240910735475524 2306 44-45 0.36275146522416435 2306 46-47 0.41940190545692246 2306 48-49 0.3061208383981082 2306 50-51 0.4775218755449373 2306 52-53 0.42442601980264527 2306 54-55 0.4645891139540481 2306 56-57 0.4835488427958481 2306 58-59 0.4934413475218733 2306 60-61 0.46178657883760366 2306 62-63 0.3512848106992834 2306 64-65 0.3436403630659228 2306 66-67 0.3758248559998023 2306 68-69 0.4920815496383284 2306 70-71 0.5171871306530562 2306 72-73 0.39115187481852587 2306 74-75 0.47900456515718304 2306 76-77 0.3924194024248422 2306 78-79 0.44755140760274514 2306 80-81 0.4292278095949982 2306 82-83 0.4629781467081884 2306 84-85 0.46961205841882503 2306 86-87 0.35440528396322435 2306 88-89 0.4193700704290393 2306 90-91 0.3694729041604745 2306 92-93 0.28952207767002136 2306 94-95 0.32629535073173344 2306 96-97 0.3522760555336859 2306 98-99 0.26194715921012346 2306 100-101 0.14252623983488633 2307 1 0.2634936405636026 2307 2 0.20241115898668482 2307 3 0.2587248677257534 2307 4 0.2846294280163164 2307 5 0.3310068319229842 2307 6 0.3221204589704527 2307 7 0.3403310476016017 2307 8 0.3386283462802311 2307 9 0.3387164221978409 2307 10-11 0.30093277201198276 2307 12-13 0.22717614531482866 2307 14-15 0.33093030930373857 2307 16-17 0.3442140333941026 2307 18-19 0.3218082141597307 2307 20-21 0.3090329000781651 2307 22-23 0.35427047392708033 2307 24-25 0.3940359236502502 2307 26-27 0.47042051267956 2307 28-29 0.26606489431377867 2307 30-31 0.15310539503084897 2307 32-33 0.13528613566499814 2307 34-35 0.15949503915707908 2307 36-37 0.24184337885213125 2307 38-39 0.1422570414130817 2307 40-41 0.1657162841730795 2307 42-43 0.11312336057936534 2307 44-45 0.14346542072524926 2307 46-47 0.07225109897763105 2307 48-49 0.052964555459858786 2307 50-51 0.0880829562151888 2307 52-53 0.2227696018550418 2307 54-55 0.2198834240025036 2307 56-57 0.10815528950271869 2307 58-59 0.12952984336056517 2307 60-61 0.19008951129033846 2307 62-63 0.22191866409772842 2307 64-65 0.1700423721208537 2307 66-67 0.16402516273775092 2307 68-69 0.21733881171483915 2307 70-71 0.15459284636595072 2307 72-73 0.07060209730483535 2307 74-75 0.19900033913705784 2307 76-77 0.12841414636399406 2307 78-79 0.17074114515531846 2307 80-81 0.006812597741962634 2307 82-83 -0.03006990545628696 2307 84-85 0.15087332901187978 2307 86-87 0.18558828841543829 2307 88-89 0.2955815364238461 2307 90-91 0.07406437992226245 2307 92-93 0.12210717387814185 2307 94-95 0.08459330872046422 2307 96-97 0.1663236751720305 2307 98-99 0.12442038993634696 2307 100-101 -0.09498953020841938 2201 1 0.3127376056611908 2201 2 0.2820851033680185 2201 3 0.3082512748534896 2201 4 0.3681593744686964 2201 5 0.4079740642238079 2201 6 0.4785615124436404 2201 7 0.3532714508907375 2201 8 0.36015664135550907 2201 9 0.3989697602933546 2201 10-11 0.39507900947447183 2201 12-13 0.4195920623128444 2201 14-15 0.5183099685153536 2201 16-17 0.5255749462252126 2201 18-19 0.4668170970914076 2201 20-21 0.4921234698225021 2201 22-23 0.4642216365554148 2201 24-25 0.5200952255135576 2201 26-27 0.6143746376314567 2201 28-29 0.43749066591328045 2201 30-31 0.47150896959102795 2201 32-33 0.47065334973259354 2201 34-35 0.3636423498415695 2201 36-37 0.32487075736118953 2201 38-39 0.32805451557160836 2201 40-41 0.19569019218410233 2201 42-43 0.2713184973449714 2201 44-45 0.44669165496422636 2201 46-47 0.3450599037064279 2201 48-49 0.3501086212825939 2201 50-51 0.23129525010800478 2201 52-53 0.2984344465446256 2201 54-55 0.4167682624720186 2201 56-57 0.4481650062009521 2201 58-59 0.3723608934044407 2201 60-61 0.4079232876643317 2201 62-63 0.3753634471135854 2201 64-65 0.4774325827589436 2201 66-67 0.37706439669572234 2201 68-69 0.38003258550772046 2201 70-71 0.31649598442481164 2201 72-73 0.4116473866968491 2201 74-75 0.34943846567608716 2201 76-77 0.43721849048975514 2201 78-79 0.32733582516686255 2201 80-81 0.49716100445925093 2201 82-83 0.46936876865677135 2201 84-85 0.4995548430704204 2201 86-87 0.5317965834984051 2201 88-89 0.6286048759253191 2201 90-91 0.5336283363571468 2201 92-93 0.46031145418757546 2201 94-95 0.3928333457870181 2201 96-97 0.4411951626058084 2201 98-99 0.4938059049396202 2201 100-101 0.550129559930113 1101 1 0.2588102482101462 1101 2 0.26989528432393683 1101 3 0.21529428542186224 1101 4 0.22918863512254717 1101 5 0.33866524615243065 1101 6 0.32035386574748514 1101 7 0.32050917523170597 1101 8 0.4137785293516103 1101 9 0.23307837498306583 1101 10-11 0.33561418365972173 1101 12-13 0.3314025976349839 1101 14-15 0.4067994536360402 1101 16-17 0.46442083643410825 1101 18-19 0.3638888214276861 1101 20-21 0.3363086521542016 1101 22-23 0.43770159221993765 1101 24-25 0.492744567689698 1101 26-27 0.4656799827695366 1101 28-29 0.30938294724325743 1101 30-31 0.2492493129762252 1101 32-33 0.34060586393746206 1101 34-35 0.2830815923639278 1101 36-37 0.30159048453528925 1101 38-39 0.2808787457768531 1101 40-41 0.14876242461370026 1101 42-43 0.22171651881227916 1101 44-45 0.2954559922263158 1101 46-47 0.20909866097801455 1101 48-49 0.243385253013642 1101 50-51 0.14231013042189034 1101 52-53 0.3132124561359717 1101 54-55 0.2750124373968532 1101 56-57 0.351993280042322 1101 58-59 0.36628556063129025 1101 60-61 0.3994237308927495 1101 62-63 0.40424979713972675 1101 64-65 0.4055044498160427 1101 66-67 0.42034872380316557 1101 68-69 0.22882723515518677 1101 70-71 0.2981727789766637 1101 72-73 0.24309306735334246 1101 74-75 0.42629957847623245 1101 76-77 0.34868692889128283 1101 78-79 0.2801542815844158 1101 80-81 0.4392776100711231 1101 82-83 0.5402692576063757 1101 84-85 0.47371944365331586 1101 86-87 0.4269395921000907 1101 88-89 0.5828352940792598 1101 90-91 0.4862765111455758 1101 92-93 0.39658670075086633 1101 94-95 0.43457070376311435 1101 96-97 0.4904327610060406 1101 98-99 0.367266942427932 1101 100-101 0.4500399128546917 2304 1 0.24801249509898327 2304 2 0.3220368849355566 2304 3 0.26488574659541086 2304 4 0.4039268018822071 2304 5 0.3870404607094855 2304 6 0.3990114525403712 2304 7 0.3574010786030186 2304 8 0.4150664906359296 2304 9 0.43969806569648284 2304 10-11 0.38085679053912 2304 12-13 0.39689981719754996 2304 14-15 0.5733411034234308 2304 16-17 0.5490962229782994 2304 18-19 0.4886492496154773 2304 20-21 0.48289023164414857 2304 22-23 0.48457173787328145 2304 24-25 0.57571873717621 2304 26-27 0.581915201757532 2304 28-29 0.5130885892666797 2304 30-31 0.4103181518004959 2304 32-33 0.3866800621525357 2304 34-35 0.5283482321945172 2304 36-37 0.4823423957525321 2304 38-39 0.4552513384798047 2304 40-41 0.45447304653781373 2304 42-43 0.3876210606131778 2304 44-45 0.3662622742604711 2304 46-47 0.4074288190875137 2304 48-49 0.5060427424794867 2304 50-51 0.47524428056252077 2304 52-53 0.5158951396476184 2304 54-55 0.5980643916855826 2304 56-57 0.5014108758336988 2304 58-59 0.5781093884272934 2304 60-61 0.5683590284680733 2304 62-63 0.5330495539928535 2304 64-65 0.5160842415617637 2304 66-67 0.5819993905574847 2304 68-69 0.5285756788828664 2304 70-71 0.5762543006253154 2304 72-73 0.5801242167046894 2304 74-75 0.48489084627630064 2304 76-77 0.5099943043606885 2304 78-79 0.5694759248577341 2304 80-81 0.46154296544077766 2304 82-83 0.5029205147260853 2304 84-85 0.44125861032033953 2304 86-87 0.35928922501063454 2304 88-89 0.4903843727378785 2304 90-91 0.5564610953093521 2304 92-93 0.5365158056792438 2304 94-95 0.560858312877194 2304 96-97 0.607477758118236 2304 98-99 0.4308142913970556 2304 100-101 0.35820023099461906 2202 1 0.20754681821918552 2202 2 0.11587522238476566 2202 3 0.17033084016472344 2202 4 0.20117745545719856 2202 5 0.23236195552419758 2202 6 0.2164965268218566 2202 7 0.259610990791181 2202 8 0.14903905967121034 2202 9 0.219440781237779 2202 10-11 0.20926642670087858 2202 12-13 0.22495538165335205 2202 14-15 0.3618604539504702 2202 16-17 0.24798614429614219 2202 18-19 0.27172868849627463 2202 20-21 0.2778172080632402 2202 22-23 0.23802331398881194 2202 24-25 0.2805032344446232 2202 26-27 0.3491463024731516 2202 28-29 0.23290493558369718 2202 30-31 0.15013252819560563 2202 32-33 0.23842251345531196 2202 34-35 0.21299929692148112 2202 36-37 0.22123399427294999 2202 38-39 0.2103351016951862 2202 40-41 0.19449426473277498 2202 42-43 0.1442935381538959 2202 44-45 0.2086699064815889 2202 46-47 0.23006159993290964 2202 48-49 0.23661506017171519 2202 50-51 0.3247084657605015 2202 52-53 0.34018728302154244 2202 54-55 0.3559208397581628 2202 56-57 0.29500938122467346 2202 58-59 0.22385425822455574 2202 60-61 0.2626042457706319 2202 62-63 0.326289834228092 2202 64-65 0.4154201642743729 2202 66-67 0.39155434247833654 2202 68-69 0.4777884419198557 2202 70-71 0.38804388764977915 2202 72-73 0.392821906081231 2202 74-75 0.31958579912278395 2202 76-77 0.2922543269317188 2202 78-79 0.3878502725188895 2202 80-81 0.32476060739679014 2202 82-83 0.44557921309820614 2202 84-85 0.35204571279069086 2202 86-87 0.2794624120001217 2202 88-89 0.549963711342528 2202 90-91 0.45204803512270075 2202 92-93 0.356739229632943 2202 94-95 0.38588316435181014 2202 96-97 0.2849263057755138 2202 98-99 0.3034134688557657 2202 100-101 0.5314314843989649 1102 1 0.1526404125030396 1102 2 0.174070833618309 1102 3 0.17150996247581674 1102 4 0.23413710935174947 1102 5 0.30051089818813637 1102 6 0.19134171789154664 1102 7 0.18431889207848684 1102 8 0.21107147846048235 1102 9 0.256548062054172 1102 10-11 0.3213658415864131 1102 12-13 0.24401769681894336 1102 14-15 0.33300583154920815 1102 16-17 0.2664672452135477 1102 18-19 0.25147916612278465 1102 20-21 0.3245774439356097 1102 22-23 0.3432796871894439 1102 24-25 0.3523000750615921 1102 26-27 0.33330997342354607 1102 28-29 0.3787718577331063 1102 30-31 0.3699972543972123 1102 32-33 0.3717627114649176 1102 34-35 0.33971684154431614 1102 36-37 0.32186973665849195 1102 38-39 0.2492637675277578 1102 40-41 0.27003078185355633 1102 42-43 0.19845598364353378 1102 44-45 0.3015754020479591 1102 46-47 0.25310239549873614 1102 48-49 0.19798781875572757 1102 50-51 0.2321446693744349 1102 52-53 0.29064457002512256 1102 54-55 0.34437036690773937 1102 56-57 0.3865810185383012 1102 58-59 0.49908259854271364 1102 60-61 0.431132459926971 1102 62-63 0.418754590628275 1102 64-65 0.4636975534905119 1102 66-67 0.5397585858364238 1102 68-69 0.5910904477752581 1102 70-71 0.5161177860021198 1102 72-73 0.4413300517270571 1102 74-75 0.5344826931075488 1102 76-77 0.5041928224267451 1102 78-79 0.39507214402127744 1102 80-81 0.552262978405075 1102 82-83 0.4430710610144146 1102 84-85 0.43359705626473755 1102 86-87 0.3596289923155993 1102 88-89 0.46039248710724223 1102 90-91 0.5317361093234894 1102 92-93 0.48143435229167864 1102 94-95 0.4856580149477807 1102 96-97 0.5082092589727196 1102 98-99 0.49629904413473014 1102 100-101 0.5315133439791282 2305 1 0.19807374603622918 2305 2 0.2405757030942013 2305 3 0.2702428652064981 2305 4 0.35171965604010325 2305 5 0.32346756983120173 2305 6 0.41392877131831085 2305 7 0.318664235862542 2305 8 0.2986671556461076 2305 9 0.34058160292302375 2305 10-11 0.3515959279267804 2305 12-13 0.3524600369767654 2305 14-15 0.41146487105005036 2305 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259.0 5 234.0 6 440.0 7 1427.0 8 4905.0 9 7113.0 10 8354.0 11 8102.0 12 6436.0 13 5946.0 14 5705.0 15 6011.0 16 6434.0 17 6838.0 18 7776.0 19 8592.0 20 9963.0 21 11646.0 22 13413.0 23 14612.0 24 16173.0 25 19069.0 26 22976.0 27 28314.0 28 34834.0 29 42638.0 30 53860.0 31 71956.0 32 98941.0 33 143873.0 34 219661.0 35 371324.0 36 744710.0 37 1443732.0 38 496440.0 39 2165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.88184110903745 32.24668256573476 15.62435162962062 19.24712469560717 2 31.28859592102056 24.643851060372146 29.62264869586414 14.444904322743154 3 32.76161235266585 23.35575645153266 24.01563171056359 19.8669994852379 4 28.853775708971874 22.81840846913045 28.48294918897405 19.844866632923626 5 30.91197163164499 21.101553501528418 28.437128161020063 19.54934670580652 6 28.422691093657136 21.434463260627993 30.300210695105978 19.842634950608893 7 31.823152057432154 21.94073121218413 24.444961107301005 21.791155623082716 8 27.857828498564214 25.394174926814756 25.020038060894382 21.72795851372664 9 26.307675911052915 23.216884009977747 28.314644092398183 22.160795986571152 10-11 22.26715311280364 29.04558161674073 31.00246673760884 17.684798532846784 12-13 22.596872546048626 28.313846389687097 30.09404138094868 18.995239683315592 14-15 22.367290669099024 28.11646539561734 30.56396247001111 18.952281465272527 16-17 22.531545689284542 27.813922690409875 30.759261430632982 18.895270189672598 18-19 22.09026080042817 27.80685260145055 30.88809589941996 19.21479069870132 20-21 22.148537950255058 28.273658843470344 30.32454832061528 19.253254885659317 22-23 22.216540844084225 28.30682739991539 29.999613113793384 19.477018642207003 24-25 21.6202790383296 28.51807203773716 30.240862950363102 19.620785973570147 26-27 21.517929352449336 28.7074716700158 30.479918223118997 19.294680754415868 28-29 21.403944064625684 29.11775149101227 30.06941632984173 19.40888811452031 30-31 21.242623952209115 29.439080655372123 30.101602617627844 19.216692774790918 32-33 20.760836559388423 29.533161135893256 30.428570884064026 19.27743142065429 34-35 20.825659773675724 29.828727164807734 30.413709043010556 18.931904018505982 36-37 20.86016396597709 29.35762101335725 30.87890819772719 18.903306822938468 38-39 21.01174835443366 28.886082373554643 30.882605022273168 19.219564249738525 40-41 21.026074841270205 29.3239922799195 30.00214249007669 19.6477903887336 42-43 21.427470003883588 28.874019806939227 30.039147339296647 19.659362849880537 44-45 20.883133353835362 29.033769890991902 30.347865205092294 19.73523155008044 46-47 21.10779076777894 29.229714385080648 30.09395841434165 19.568536432798766 48-49 20.86768452170209 29.653146296476564 29.845789141800843 19.633380040020505 50-51 20.983409139991124 29.37701939059862 29.508324665448015 20.13124680396224 52-53 21.258129610369707 29.13713617328527 29.455756020242507 20.148978196102515 54-55 21.54555561908755 29.12496108665129 29.45276078296834 19.87672251129281 56-57 21.569383152817426 28.866457039662823 29.5693816274562 19.994778180063555 58-59 21.52918643016558 29.313679137370936 29.448998734119296 19.708135698344186 60-61 21.056183661046898 29.587794045545547 29.10503786949486 20.250984423912698 62-63 21.114379074589802 29.504180572800397 29.08905112270529 20.29238922990451 64-65 20.91974289271106 29.397677904524784 29.28943624399787 20.39314295876629 66-67 21.25184274996989 29.029352891764454 29.290768032270805 20.428036325994857 68-69 21.28295147878986 29.13634464241769 29.707242944257278 19.873460934535174 70-71 21.198096742971387 29.912293180842443 29.266412797447423 19.62319727873875 72-73 20.94143318690381 30.11767629023075 29.03850907628469 19.902381446580748 74-75 21.10951114805592 29.60695360654763 28.966526616266137 20.317008629130314 76-77 21.159282802662503 29.351651767504734 28.999079412048285 20.489986017784485 78-79 20.876750529123754 29.016027358819773 29.724083289369368 20.38313882268711 80-81 20.83735835348967 29.41447248804755 29.422515116775028 20.325654041687752 82-83 20.420225337930013 29.709426382158497 29.2617927786184 20.608555501293086 84-85 20.474742946369073 29.691174758855766 29.40089184102194 20.43319045375322 86-87 20.624520127269683 29.458638751173233 29.90167835050603 20.015162771051052 88-89 20.797600496795972 29.979520000243976 29.53745341743556 19.685426085524487 90-91 21.099388219535662 29.713401470417484 29.037988622138478 20.149221687908373 92-93 20.80568103963564 29.769799973733228 29.099489322203066 20.325029664428065 94-95 20.439234077796435 29.920354049748866 29.076056166273307 20.564355706181388 96-97 20.556169089933416 30.311844106524244 28.926749499099806 20.205237304442534 98-99 20.862018238416532 30.06687470209875 28.832814006973813 20.238293052510908 100-101 20.94600449968856 30.087721817288653 28.445256711730476 20.521016971292312 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2271.0 1 1406.0 2 476.0 3 360.0 4 279.5 5 274.0 6 352.5 7 504.0 8 698.5 9 903.0 10 1107.5 11 1277.5 12 1370.0 13 1284.0 14 1072.5 15 923.0 16 828.5 17 746.5 18 737.0 19 787.5 20 975.0 21 1302.0 22 1722.5 23 2430.5 24 3526.0 25 5299.0 26 8341.5 27 12752.5 28 17732.0 29 23102.5 30 29079.5 31 33809.5 32 41331.5 33 56781.0 34 78570.0 35 111446.0 36 155202.5 37 206434.0 38 258557.5 39 278385.0 40 284663.0 41 274166.5 42 241257.5 43 226070.0 44 213271.0 45 204021.5 46 209127.5 47 195164.0 48 159368.5 49 129477.0 50 103330.0 51 83479.5 52 68749.0 53 55007.0 54 45000.5 55 34980.5 56 24016.0 57 15360.5 58 10260.0 59 7386.5 60 5548.0 61 4159.0 62 2984.0 63 2024.5 64 1431.0 65 1050.0 66 795.0 67 607.5 68 484.0 69 365.5 70 261.0 71 209.0 72 155.5 73 119.5 74 102.0 75 82.0 76 66.5 77 51.5 78 43.0 79 41.5 80 36.0 81 24.0 82 19.5 83 16.5 84 16.0 85 20.0 86 17.0 87 12.0 88 10.5 89 11.0 90 8.5 91 8.5 92 9.5 93 5.5 94 4.5 95 3.0 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.266217997359855 2 0.25398478522734524 3 0.21741178663657076 4 0.22009650813977166 5 0.23379871883063663 6 0.2381044042602984 7 0.21411920366094703 8 0.20935762212696807 9 0.18990605500943702 10-11 0.17805275629719158 12-13 0.20753403770969953 14-15 0.20411481692732106 16-17 0.2054065225562196 18-19 0.19665585010946574 20-21 0.19773227146688116 22-23 0.16565491501590063 24-25 0.4754489816800683 26-27 0.3335766467727115 28-29 0.27885645047398 30-31 0.2815285081965054 32-33 0.32578842165729377 34-35 0.32105216768466577 36-37 0.313821148918969 38-39 0.3387941244110076 40-41 0.3443028690048396 42-43 0.38119246211250096 44-45 0.3469369353853386 46-47 0.37415140005693637 48-49 0.34914043322287136 50-51 0.3719732297807545 52-53 0.36141163669740756 54-55 0.33541289497065546 56-57 0.3741767276182873 58-59 0.3608670941283621 60-61 0.32531986177230116 62-63 0.3015372816637574 64-65 0.30818576651838225 66-67 0.31846875642686867 68-69 0.32428143175691215 70-71 0.3003342224995872 72-73 0.307248646748397 74-75 0.30560235526058516 76-77 0.3089076020168844 78-79 0.3110097896090134 80-81 0.32894170304548725 82-83 0.3466963235525045 84-85 0.3415674923789368 86-87 0.3452399887748248 88-89 0.34074434663503084 90-91 0.31352988196343307 92-93 0.29721893245342007 94-95 0.3064128372238156 96-97 0.2939390132584718 98-99 0.3140617607518031 100-101 0.33759106524683735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3948268.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.38207568229818 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6388029825536 38.01953183695159 2 5.801839084729419 5.0339164454049445 3 1.8088003785992315 2.3540854475568436 4 0.8353045781410866 1.4494898570674708 5 0.6179452466413943 1.3403873728656688 6 0.4899842261078372 1.2753919668085498 7 0.3363767595311486 1.0214905427822552 8 0.23701641265037654 0.8225811161236359 9 0.19952279402206088 0.7790141655547771 >10 1.5983669924409305 14.045945456109818 >50 0.24057183247071698 7.284394288074384 >100 0.16230795090906963 13.656953073543262 >500 0.02347937264934584 7.085488291359172 >1k 0.009681388553915156 5.831330139797671 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.104819632304595E-4 0.0 0.0 2 8.864646472833152E-4 0.0 0.0 3 0.001266378067547593 0.0 0.0 4 0.001468998558355208 0.0 0.0 5 0.0019502222240232933 0.0 0.0 6 0.0027860317486047047 0.0 0.0 7 0.0037231515185899237 0.0 0.0 8 0.004964202024786564 0.0 0.0 9 0.005420098129103698 0.0 0.0 10-11 0.007294337669074136 0.0 0.0 12-13 0.010548929302671449 0.0 0.0 14-15 0.014867278513008743 0.0 0.0 16-17 0.021123186166693852 0.0 0.0 18-19 0.028936738843462505 0.0 0.0 20-21 0.03757343726413709 0.0 2.532756135095186E-5 22-23 0.04916079658219756 0.0 5.065512270190372E-5 24-25 0.06220449067793777 0.0 7.598268405285558E-5 26-27 0.07672984711270867 0.0 7.598268405285558E-5 28-29 0.09253424539570262 0.0 7.598268405285558E-5 30-31 0.11206179519728651 0.0 7.598268405285558E-5 32-33 0.1307915268163154 0.0 7.598268405285558E-5 34-35 0.1514208255366657 0.0 7.598268405285558E-5 36-37 0.17521606942588497 0.0 7.598268405285558E-5 38-39 0.2013541127400673 0.0 7.598268405285558E-5 40-41 0.23111399732743573 0.0 7.598268405285558E-5 42-43 0.2644070767232619 0.0 7.598268405285558E-5 44-45 0.30218313447820666 0.0 7.598268405285558E-5 46-47 0.3418460955537973 0.0 7.598268405285558E-5 48-49 0.3847763120436607 0.0 7.598268405285558E-5 50-51 0.43230348091872184 0.0 7.598268405285558E-5 52-53 0.4845162486437091 0.0 7.598268405285558E-5 54-55 0.5400342631249956 0.0 7.598268405285558E-5 56-57 0.5973758620235505 0.0 7.598268405285558E-5 58-59 0.6603655071033678 0.0 7.598268405285558E-5 60-61 0.7255966413627444 0.0 7.598268405285558E-5 62-63 0.7973749502313419 0.0 7.598268405285558E-5 64-65 0.8727497728117747 0.0 7.598268405285558E-5 66-67 0.9502014554229854 0.0 7.598268405285558E-5 68-69 1.0349221481419195 0.0 7.598268405285558E-5 70-71 1.1245943791049644 0.0 7.598268405285558E-5 72-73 1.2215482839564082 0.0 7.598268405285558E-5 74-75 1.3231118049737252 0.0 8.86464647283315E-5 76-77 1.4320836376861954 0.0 1.0131024540380744E-4 78-79 1.5501354011429822 0.0 1.0131024540380744E-4 80-81 1.6761526826446431 0.0 1.266378067547593E-4 82-83 1.8095529482801065 0.0 1.266378067547593E-4 84-85 1.9564021489929255 0.0 1.266378067547593E-4 86-87 2.11364831364031 0.0 1.266378067547593E-4 88-89 2.284368740926401 0.0 1.266378067547593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 410 0.0 45.11191 9 CGGACGG 65 2.6089056E-6 43.802254 1 CGAGGCG 210 0.0 42.93316 1 GAACCGG 70 4.342657E-6 40.673523 1 CGTGCGG 60 8.3435225E-5 39.5437 1 CGCGGAG 110 1.6389095E-9 38.824726 1 GTCGACC 50 0.0016242664 37.96195 1 GCGCACC 105 4.7079084E-8 36.150566 2 GATCCGG 160 1.8189894E-12 35.58933 1 CGACGGG 190 0.0 34.964954 1 AGAGCGG 530 0.0 34.90144 8 CGAGGGG 450 0.0 34.79846 1 CGGTGCG 55 0.0025930894 34.510864 1 GTCGGGC 125 5.7152647E-9 34.165756 1 CGGGTCG 70 2.0663574E-4 33.8946 1 CGACGCC 70 2.0663574E-4 33.8946 1 GGTCCGG 70 2.0663574E-4 33.8946 1 CGCGCTG 70 2.0663574E-4 33.8946 1 CGCGGCT 70 2.0688243E-4 33.88772 3 CGGAAAG 225 0.0 33.734535 5 >>END_MODULE