FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948268
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4100.045.111919
CGGACGG652.6089056E-643.8022541
CGAGGCG2100.042.933161
GAACCGG704.342657E-640.6735231
CGTGCGG608.3435225E-539.54371
CGCGGAG1101.6389095E-938.8247261
GTCGACC500.001624266437.961951
GCGCACC1054.7079084E-836.1505662
GATCCGG1601.8189894E-1235.589331
CGACGGG1900.034.9649541
AGAGCGG5300.034.901448
CGAGGGG4500.034.798461
CGGTGCG550.002593089434.5108641
GTCGGGC1255.7152647E-934.1657561
CGGGTCG702.0663574E-433.89461
CGACGCC702.0663574E-433.89461
GGTCCGG702.0663574E-433.89461
CGCGCTG702.0663574E-433.89461
CGCGGCT702.0688243E-433.887723
CGGAAAG2250.033.7345355
Produced by FastQC (version 0.11.2)