##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3136301 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.095582981352873 33.0 33.0 33.0 27.0 33.0 2 30.584440715352258 33.0 33.0 33.0 27.0 33.0 3 30.446115981852508 33.0 33.0 33.0 22.0 33.0 4 33.78320798928419 37.0 37.0 37.0 22.0 37.0 5 33.84537389746711 37.0 37.0 37.0 22.0 37.0 6 34.47841135146148 37.0 37.0 37.0 27.0 37.0 7 33.88041581468105 37.0 37.0 37.0 22.0 37.0 8 34.232991667572726 37.0 37.0 37.0 27.0 37.0 9 33.55945204239006 37.0 37.0 37.0 22.0 37.0 10-11 33.589245738849684 37.0 37.0 37.0 18.5 37.0 12-13 33.598632752404825 37.0 37.0 37.0 18.5 37.0 14-15 35.17232705024167 40.0 37.0 40.0 18.5 40.0 16-17 35.087783028478455 40.0 37.0 40.0 18.5 40.0 18-19 35.14437373836249 40.0 37.0 40.0 18.5 40.0 20-21 35.12622672377428 40.0 37.0 40.0 18.5 40.0 22-23 35.9559724975377 40.0 37.0 40.0 27.0 40.0 24-25 35.254994657719394 40.0 37.0 40.0 22.0 40.0 26-27 35.11004173387695 40.0 37.0 40.0 21.0 40.0 28-29 34.75202906226156 40.0 37.0 40.0 15.0 40.0 30-31 34.74303789719163 40.0 37.0 40.0 15.0 40.0 32-33 34.502814621428236 40.0 37.0 40.0 15.0 40.0 34-35 34.77647250694369 38.5 37.0 40.0 18.5 40.0 36-37 34.4702595509806 37.0 37.0 40.0 15.0 40.0 38-39 34.39873947047812 37.0 37.0 40.0 15.0 40.0 40-41 34.25054562683875 37.0 37.0 40.0 15.0 40.0 42-43 34.11362637068317 37.0 37.0 40.0 15.0 40.0 44-45 33.97485875877347 37.0 35.0 40.0 15.0 40.0 46-47 33.92601268181849 37.0 33.0 40.0 15.0 40.0 48-49 33.81765398155343 37.0 33.0 40.0 15.0 40.0 50-51 32.87579620068354 37.0 33.0 38.5 15.0 38.5 52-53 33.3090677521067 37.0 33.0 38.5 15.0 40.0 54-55 33.917127055088145 37.0 33.0 40.0 15.0 40.0 56-57 34.227099216561164 37.0 33.0 40.0 18.5 40.0 58-59 33.642481860000046 37.0 33.0 40.0 15.0 40.0 60-61 33.43195009024963 37.0 33.0 40.0 15.0 40.0 62-63 33.04483354754534 37.0 33.0 40.0 6.0 40.0 64-65 32.82859393916591 37.0 33.0 38.5 10.5 40.0 66-67 32.7078898039442 37.0 33.0 37.0 15.0 40.0 68-69 32.797305169369906 37.0 33.0 37.0 15.0 40.0 70-71 32.365908437997504 37.0 33.0 37.0 15.0 40.0 72-73 32.65877414189518 37.0 33.0 37.0 18.5 37.0 74-75 32.75220363096527 37.0 33.0 37.0 22.0 37.0 76-77 32.44336497039028 37.0 33.0 37.0 22.0 37.0 78-79 31.94318290878331 37.0 33.0 37.0 22.0 37.0 80-81 31.438785052837723 37.0 33.0 37.0 15.0 37.0 82-83 31.036957071403542 35.0 33.0 37.0 15.0 37.0 84-85 30.84411764049433 33.0 33.0 37.0 15.0 37.0 86-87 30.676519090482707 33.0 33.0 37.0 15.0 37.0 88-89 30.47957083838573 33.0 27.0 37.0 15.0 37.0 90-91 30.409321681815616 33.0 27.0 37.0 15.0 37.0 92-93 30.250495886714955 33.0 27.0 37.0 10.5 37.0 94-95 30.12061868423981 33.0 27.0 37.0 6.0 37.0 96-97 29.984099102732806 33.0 27.0 37.0 6.0 37.0 98-99 29.85732252739772 33.0 27.0 37.0 6.0 37.0 100-101 28.694031440222098 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 -0.016652492877526015 2306 2 -0.12208370196597329 2306 3 -0.26102424309247496 2306 4 0.05351704361617493 2306 5 -0.03673022192274544 2306 6 -0.24886148774612593 2306 7 0.4465141700262336 2306 8 -0.2485997634173529 2306 9 -0.01728795575493791 2306 10-11 -0.037005276035422696 2306 12-13 -0.03133824796872631 2306 14-15 0.30269092399082353 2306 16-17 0.11379009399612272 2306 18-19 0.14013787793387422 2306 20-21 0.16807741378189434 2306 22-23 -0.011846193333347799 2306 24-25 0.3945558088820533 2306 26-27 0.032012505213330655 2306 28-29 0.19742618966346015 2306 30-31 0.26166333443043754 2306 32-33 0.31726564558660897 2306 34-35 0.1048531571153859 2306 36-37 0.43214039562155193 2306 38-39 0.19767849264466264 2306 40-41 0.17542865477558678 2306 42-43 0.19131395512712146 2306 44-45 0.2084509597854236 2306 46-47 0.22087303918936385 2306 48-49 0.1139695621203245 2306 50-51 0.15141901567224636 2306 52-53 0.07310547301126036 2306 54-55 0.19475879532544127 2306 56-57 0.20323193283898178 2306 58-59 0.3333422387130014 2306 60-61 0.22074590565268437 2306 62-63 0.38092677145942844 2306 64-65 0.3519955449050016 2306 66-67 0.15648285009464047 2306 68-69 0.06306009765241782 2306 70-71 0.22713107927349085 2306 72-73 -0.10214502756744537 2306 74-75 0.0667719003843743 2306 76-77 0.24631002051670237 2306 78-79 0.2529751930626105 2306 80-81 0.31665424793402863 2306 82-83 0.4229996225678647 2306 84-85 0.2707586490117677 2306 86-87 0.39710752752064593 2306 88-89 0.48939757150035845 2306 90-91 0.3156693600415359 2306 92-93 0.3272292909236967 2306 94-95 0.23380468001201038 2306 96-97 0.26782230366525184 2306 98-99 0.2671017361827843 2306 100-101 0.3054112136047138 2307 1 0.1770839719865407 2307 2 0.11714574025853253 2307 3 0.07579885800966224 2307 4 0.25803969738384325 2307 5 0.21126330963224405 2307 6 0.038982894426432324 2307 7 0.5909557408693473 2307 8 -0.036322649094124415 2307 9 0.32691374832222664 2307 10-11 0.1316730694522974 2307 12-13 0.14660367244325556 2307 14-15 0.33039034876531304 2307 16-17 0.27137318054710846 2307 18-19 0.22237857172859776 2307 20-21 0.2067434959391079 2307 22-23 0.09710738790020912 2307 24-25 0.4331547482389624 2307 26-27 0.1393912502895276 2307 28-29 0.3260412432924369 2307 30-31 0.3862704247101547 2307 32-33 0.4046088369514038 2307 34-35 0.1930638760904415 2307 36-37 0.4336150504578242 2307 38-39 0.129097983808812 2307 40-41 0.34438693955767974 2307 42-43 0.291764913173175 2307 44-45 0.31751395891576095 2307 46-47 0.22552685233201686 2307 48-49 0.31174738410734193 2307 50-51 0.33399769708712057 2307 52-53 0.23760639951084528 2307 54-55 0.32688872126231416 2307 56-57 0.26433507839035286 2307 58-59 0.40584189767020007 2307 60-61 0.3082585526563335 2307 62-63 0.4845849527918773 2307 64-65 0.347044029822392 2307 66-67 0.2733962280666873 2307 68-69 0.023443358703623574 2307 70-71 0.25490155463030817 2307 72-73 -0.05248955184116966 2307 74-75 0.16370834137544676 2307 76-77 0.2944357772054076 2307 78-79 0.37428518106926845 2307 80-81 0.2732316845426972 2307 82-83 0.4692119870744058 2307 84-85 0.42182355513973846 2307 86-87 0.43656866569413566 2307 88-89 0.6076509634551606 2307 90-91 0.45756732003721723 2307 92-93 0.3464690781129889 2307 94-95 0.3433653884715717 2307 96-97 0.5265444731521534 2307 98-99 0.40586636549768684 2307 100-101 0.29339691538552515 2201 1 0.001713201745634052 2201 2 -0.23875747703212014 2201 3 -0.22443128691394776 2201 4 -0.06580467049604266 2201 5 -0.11878380238741215 2201 6 -0.050328227665453085 2201 7 0.3028698020472831 2201 8 -0.2220347107188161 2201 9 0.08318553593908007 2201 10-11 -0.046550651720359326 2201 12-13 -0.013305081863499879 2201 14-15 0.3205565607829328 2201 16-17 0.2770518373130315 2201 18-19 0.11239975859950846 2201 20-21 0.025336836628049753 2201 22-23 0.12690946326589625 2201 24-25 0.31365487346487697 2201 26-27 0.04864290699453022 2201 28-29 0.07103643732491349 2201 30-31 0.10247398112612416 2201 32-33 0.17054755748100803 2201 34-35 0.007583143958825644 2201 36-37 0.2607344144455581 2201 38-39 0.19412712421752332 2201 40-41 0.17668828725928165 2201 42-43 0.1740830140714138 2201 44-45 0.27570322603956043 2201 46-47 0.050673738945029356 2201 48-49 0.10990114799219697 2201 50-51 0.374144448320763 2201 52-53 0.24869227741219646 2201 54-55 0.38807461067090543 2201 56-57 0.353011271251809 2201 58-59 0.3196989912105721 2201 60-61 0.2601416796489531 2201 62-63 0.34010690794069376 2201 64-65 0.42626651227383405 2201 66-67 0.2559683345019508 2201 68-69 0.30981776388053106 2201 70-71 0.34990910203000425 2201 72-73 0.0768104352977943 2201 74-75 0.29547561747489226 2201 76-77 0.36704226999983547 2201 78-79 0.4293810296609877 2201 80-81 0.7016850429663428 2201 82-83 0.7418863387764603 2201 84-85 0.5036713819624516 2201 86-87 0.5666321924620199 2201 88-89 0.5713667547814048 2201 90-91 0.4442321139559766 2201 92-93 0.24412752273944704 2201 94-95 0.3556481891999148 2201 96-97 0.3507755183485557 2201 98-99 0.3482502607682072 2201 100-101 0.060315105634149546 1101 1 -0.004645324663716366 1101 2 -0.15104070908634526 1101 3 -0.09624058809625424 1101 4 -0.08125210818619877 1101 5 -0.028428134474360434 1101 6 -0.05632748862370107 1101 7 0.3232209608289409 1101 8 -0.008534827109748733 1101 9 0.01899975972547452 1101 10-11 -0.11308809460333435 1101 12-13 -0.05878154403481517 1101 14-15 0.17073244946436716 1101 16-17 0.1223558292779856 1101 18-19 -0.006126097280841236 1101 20-21 -0.1088757501971429 1101 22-23 -0.08930867776735596 1101 24-25 0.03499193673433609 1101 26-27 -0.006387297674450565 1101 28-29 -0.030200480903531002 1101 30-31 -0.019219173296164627 1101 32-33 0.2151274838698427 1101 34-35 -0.049180227569181056 1101 36-37 0.21018762121934742 1101 38-39 -0.026820884032396464 1101 40-41 -0.006792664509291058 1101 42-43 0.08818365397738859 1101 44-45 0.09382301591729458 1101 46-47 0.04819225161536167 1101 48-49 0.07671678963182416 1101 50-51 0.026625935650429255 1101 52-53 0.03867175028334202 1101 54-55 0.17666243862068853 1101 56-57 0.2831255134328572 1101 58-59 0.2126440725946992 1101 60-61 0.23432462341177995 1101 62-63 0.35965391115894363 1101 64-65 0.22290720938229214 1101 66-67 0.20161848776357516 1101 68-69 0.18748299424505177 1101 70-71 0.14888038521159785 1101 72-73 -0.0012178859542117948 1101 74-75 0.16042753906484108 1101 76-77 0.3840113360561688 1101 78-79 0.18064008658511455 1101 80-81 0.36207566071734476 1101 82-83 0.4274020971325996 1101 84-85 0.3705376495899806 1101 86-87 0.3422090601295942 1101 88-89 0.5046755619126699 1101 90-91 0.38482984246068597 1101 92-93 0.40551543989514016 1101 94-95 0.31582564061280394 1101 96-97 0.17464184620635947 1101 98-99 0.18271084982029961 1101 100-101 0.23647482330066438 2304 1 0.20625672501017078 2304 2 0.06976426576673589 2304 3 0.08843419430235855 2304 4 0.21402067596342533 2304 5 0.2121355393772575 2304 6 0.24186027521380282 2304 7 0.45177038271907577 2304 8 0.19844368810959878 2304 9 0.2573894852304903 2304 10-11 0.21403628783493645 2304 12-13 0.18641407427904966 2304 14-15 0.4243854690175084 2304 16-17 0.30816779991138077 2304 18-19 0.23269931533629062 2304 20-21 0.24589343178801215 2304 22-23 0.17121996447576038 2304 24-25 0.39243820483952874 2304 26-27 0.39820491713745554 2304 28-29 0.46943092651754625 2304 30-31 0.40174339536176973 2304 32-33 0.37168253429330633 2304 34-35 0.2564486191484434 2304 36-37 0.47559714146351695 2304 38-39 0.40300685253424007 2304 40-41 0.29659008230956374 2304 42-43 0.29630583896285856 2304 44-45 0.1954896099338299 2304 46-47 0.17488702960937985 2304 48-49 0.2427976968068961 2304 50-51 0.31841336535431 2304 52-53 0.3744158571468361 2304 54-55 0.4406599109659126 2304 56-57 0.45591523289792946 2304 58-59 0.45311971158109543 2304 60-61 0.36212325605083606 2304 62-63 0.3845573790773642 2304 64-65 0.5030447631469528 2304 66-67 0.43633126190741933 2304 68-69 0.4368678185727646 2304 70-71 0.4739647946928116 2304 72-73 0.3927665549517414 2304 74-75 0.48326207368946683 2304 76-77 0.6340676755208889 2304 78-79 0.6204478144508272 2304 80-81 0.4717055638533516 2304 82-83 0.5048670804814748 2304 84-85 0.46387939324441874 2304 86-87 0.3974322057207438 2304 88-89 0.354187367274136 2304 90-91 0.613328893592989 2304 92-93 0.475953731015899 2304 94-95 0.47426012144784124 2304 96-97 0.5501392503174465 2304 98-99 0.30481121925334165 2304 100-101 0.32871007696216026 2202 1 -0.010958816177062403 2202 2 -0.19995804811008 2202 3 -0.12438582649348362 2202 4 -0.1080836043451896 2202 5 -0.13746550961933934 2202 6 0.07100830869620012 2202 7 0.2094995952617964 2202 8 -0.055426280930653604 2202 9 -0.03341494570367587 2202 10-11 -0.12717953572646223 2202 12-13 -0.11180072821114351 2202 14-15 -0.04545568381789167 2202 16-17 -0.03979399689311691 2202 18-19 -0.04534270069038371 2202 20-21 0.001458230807038774 2202 22-23 0.009753428543646692 2202 24-25 0.17703137934130098 2202 26-27 0.10225671329450847 2202 28-29 -0.0037671246327377617 2202 30-31 0.042782072041021024 2202 32-33 0.030040614367443652 2202 34-35 -0.06058111672251698 2202 36-37 0.100895798938204 2202 38-39 -0.11692607190258997 2202 40-41 -0.030845960333621747 2202 42-43 0.03399175153452205 2202 44-45 0.004695163500286981 2202 46-47 -0.021310162717846026 2202 48-49 -0.07040906738026109 2202 50-51 -0.04028410973709384 2202 52-53 -0.04781365844106489 2202 54-55 0.18145135424888537 2202 56-57 0.2000887307571162 2202 58-59 0.05503377155206124 2202 60-61 -0.06725141779531185 2202 62-63 0.051546591916491025 2202 64-65 -0.02337886673384304 2202 66-67 0.08858826607360726 2202 68-69 0.050459454057367736 2202 70-71 0.11074309275667815 2202 72-73 0.12759908445476498 2202 74-75 0.33621729086405594 2202 76-77 0.1162118002273047 2202 78-79 0.21310144510386664 2202 80-81 0.09901847732283287 2202 82-83 0.15605231090892602 2202 84-85 0.1542467682646027 2202 86-87 0.17318480695616856 2202 88-89 0.24722801433217967 2202 90-91 0.1754036260200742 2202 92-93 0.24004923276435974 2202 94-95 0.2039085110180956 2202 96-97 0.06928043505644865 2202 98-99 0.14310053107943688 2202 100-101 0.21770992641461007 1102 1 -0.049069229052431496 1102 2 -0.23021091385070136 1102 3 -0.20897526247066622 1102 4 -0.181198964990827 1102 5 -0.15213855122646436 1102 6 -0.038967844673308605 1102 7 -0.02260645856856769 1102 8 -0.11556860963861482 1102 9 -0.2058561399179979 1102 10-11 -0.1648780069695519 1102 12-13 -0.1747491777714103 1102 14-15 -0.09590461078339274 1102 16-17 -0.1657658757774172 1102 18-19 -0.0410961253304265 1102 20-21 -0.041428469660552025 1102 22-23 0.09290803030210526 1102 24-25 -0.021705358533147034 1102 26-27 0.05772646151451255 1102 28-29 -0.08136140849232731 1102 30-31 -0.04961247632980559 1102 32-33 -0.14334338331597252 1102 34-35 -0.18008219090023658 1102 36-37 0.03952588416606062 1102 38-39 -0.05032117732068997 1102 40-41 -0.02647874036227904 1102 42-43 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82282.0 11 54253.0 12 30877.0 13 20712.0 14 15224.0 15 13297.0 16 12006.0 17 10914.0 18 10681.0 19 10781.0 20 11208.0 21 12182.0 22 13291.0 23 13931.0 24 15373.0 25 17133.0 26 19887.0 27 24216.0 28 30010.0 29 36806.0 30 44242.0 31 56274.0 32 77345.0 33 111408.0 34 167388.0 35 283933.0 36 566425.0 37 1007099.0 38 278519.0 39 326.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.15543188434707 22.976919218703998 23.62115819802333 13.246490698925609 2 30.652616465367316 25.80899836380019 31.890905522645816 11.647479648186676 3 36.43824989144338 19.612499972042073 29.324923163635653 14.624326972878896 4 27.50803053536583 17.392863088358734 35.90886680382935 19.190239572446085 5 33.75092357526531 16.11817800650913 33.26264307427207 16.86825534395349 6 30.207573984844615 16.500040107981555 38.264005115924846 15.028380791248983 7 35.91570198872069 18.94609273880352 28.75572600302703 16.382479269448762 8 27.548326866747434 32.51631982251394 24.168992592819787 15.766360717918834 9 32.62045085017553 27.216924929644502 23.117427400472124 17.045196819707844 10-11 27.072522435192102 29.672120570812226 26.869386411030415 16.385970582965257 12-13 29.070033617861796 23.804337494898217 26.933282641633916 20.19234624560608 14-15 25.287639810487107 24.384115036755663 29.413954053026554 20.91429109973068 16-17 26.69960930798409 25.698518922956453 30.205853703107934 17.39601806595152 18-19 26.342644777768175 25.862742301235 30.601310056292903 17.19330286470392 20-21 25.022386026136147 28.536696630771292 29.224985073320425 17.215932269772143 22-23 28.464186602030424 25.74706917419532 28.490532897741243 17.298211326033012 24-25 24.619194140038925 29.798326805324393 28.258014588402308 17.32446446623437 26-27 22.48861146022496 31.76202246263718 28.19135817673325 17.55800790040462 28-29 23.684549987028564 30.139098885511363 27.921950149719 18.254400977741078 30-31 26.422288510388114 26.885039301607407 28.410107593675104 18.282564594329372 32-33 22.592556818854188 29.303915664288294 30.434120001394465 17.66940751546305 34-35 27.12999453901883 27.07856696817999 28.600109859269796 17.191328633531384 36-37 23.82861350493729 27.457001072124992 30.13977599703227 18.57460942590545 38-39 20.649569888259713 29.96688495460576 31.490544898835577 17.89300025829895 40-41 21.826263774000584 31.04329802192452 28.84881480376073 18.281623400314167 42-43 24.465074454442107 27.471653070220704 30.33672304451586 17.726549430821333 44-45 22.016462649689718 29.27890612841097 31.068680641519013 17.6359505803803 46-47 23.685173855278887 30.112366597464167 28.44309097099383 17.759368576263114 48-49 20.766372965595963 29.65738323031082 30.25944269949908 19.316801104594138 50-51 19.7987190836253 27.44039591902494 31.80841003147174 20.952474965878015 52-53 25.288864651419562 26.914431690622674 27.993215925937005 19.803487732020756 54-55 27.160354033932332 26.936287998265048 27.583072762606275 18.32028520519635 56-57 27.644433067212777 26.882469641457206 27.06897268931091 18.404124602019102 58-59 25.750129057471728 26.73238959407246 27.598206457080853 19.91927489137496 60-61 21.511809487409316 27.014209445191167 27.414738131543114 24.0592429358564 62-63 24.336374162343752 27.04470942757185 27.63813953577608 20.980776874308315 64-65 21.608903699892345 28.646852063946053 30.012300516340275 19.731943719821324 66-67 20.269637343513615 28.424974882704667 28.7109034886616 22.59448428512012 68-69 20.038188447514873 27.22330966545129 33.757164331862086 18.981337555171752 70-71 19.379603653520828 31.905873986498744 30.365363274438224 18.349159085542198 72-73 19.25442818175132 35.550744612472144 26.731923520928135 18.46290368484841 74-75 19.938414398604245 35.10922898242257 26.304333088598337 18.648023530374854 76-77 20.287008737377413 34.464472875275916 26.596308220202058 18.652210167144613 78-79 20.096005464646012 34.304868597297194 27.122159366640876 18.476966571415918 80-81 19.26912206401659 34.565174038013595 27.04830166386453 19.11740223410528 82-83 18.920845242988964 33.654743415233376 27.35337727709683 20.071034064680827 84-85 19.056757045948512 32.73175022043955 27.889341505923998 20.322151227687943 86-87 19.27389809525409 32.41559721145902 28.541950075295723 19.76855461799116 88-89 19.764213870568252 32.743950645204265 27.897653382458245 19.594182101769235 90-91 20.220702045682472 32.00648166151579 27.55617567899466 20.216640613807076 92-93 19.7525740297857 32.490738033225576 27.613187205320134 20.143500731668595 94-95 19.410203716497804 32.915345515082436 27.697215800447978 19.977234967971786 96-97 20.062310784309183 33.18946133049575 26.962603457628237 19.78562442756683 98-99 20.671667040831274 32.12110397106972 26.651457035349917 20.555771952749087 100-101 20.60449187371881 32.11222535146392 26.20355631059685 21.079726464220418 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2068.0 1 1337.5 2 506.0 3 434.0 4 439.5 5 417.5 6 468.0 7 630.0 8 807.0 9 963.0 10 1134.5 11 1264.5 12 1297.5 13 1243.5 14 1163.0 15 1117.5 16 1048.0 17 991.5 18 1001.0 19 1054.5 20 1149.5 21 1294.5 22 1507.5 23 1786.0 24 2282.5 25 3037.0 26 4587.5 27 7216.0 28 9628.0 29 11696.0 30 14140.5 31 16484.5 32 19934.5 33 29244.0 34 46563.0 35 76953.0 36 115883.5 37 157353.0 38 204047.5 39 234540.5 40 239385.0 41 218886.0 42 192565.5 43 186976.0 44 180642.5 45 167914.5 46 164621.5 47 151597.5 48 123302.5 49 101371.0 50 85400.0 51 73240.5 52 63084.0 53 51203.5 54 40068.5 55 30710.5 56 22900.5 57 16527.5 58 12072.5 59 9041.5 60 6759.0 61 5043.5 62 3774.5 63 2836.5 64 2103.0 65 1563.5 66 1158.5 67 858.0 68 644.0 69 501.0 70 378.0 71 267.5 72 213.5 73 167.5 74 126.0 75 108.5 76 87.5 77 70.5 78 64.5 79 51.5 80 40.0 81 42.0 82 33.0 83 29.5 84 32.5 85 27.5 86 21.0 87 16.0 88 17.5 89 12.5 90 9.5 91 8.5 92 8.0 93 8.5 94 8.0 95 5.5 96 3.0 97 3.5 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25871241312616355 2 0.24583099645091464 3 0.21037521589923924 4 0.21311729964694076 5 0.22819876025929908 6 0.23110026748070417 7 0.20725051581464918 8 0.20294608202465259 9 0.18400657334866774 10-11 0.1719860434314181 12-13 0.19986920898217359 14-15 0.19676045124495384 16-17 0.1981314931188046 18-19 0.19059076281262544 20-21 0.19130816844429155 22-23 0.15722342976646692 24-25 0.4581511787293375 26-27 0.32305572711292696 28-29 0.2638299066320484 30-31 0.26705026080086064 32-33 0.30781484302686507 34-35 0.3052481251002375 36-37 0.2975957983624658 38-39 0.32134989594429875 40-41 0.32705725630288673 42-43 0.36359711647574644 44-45 0.3308515349770319 46-47 0.35624769433801157 48-49 0.33309940595625226 50-51 0.3544462090851611 52-53 0.34261698733635576 54-55 0.3185440428071158 56-57 0.3555462310537158 58-59 0.34293583428376295 60-61 0.3087554415217162 62-63 0.28613325060317873 64-65 0.29322759518298785 66-67 0.30199588623668455 68-69 0.3093134236796787 70-71 0.28573469191891976 72-73 0.2924145354670996 74-75 0.2904376843931753 76-77 0.2939290584672836 78-79 0.2962566411833558 80-81 0.3129323365327499 82-83 0.3295602048400329 84-85 0.32396444091303733 86-87 0.3282051053135525 88-89 0.3226890531234088 90-91 0.29732477845716976 92-93 0.28251433775010754 94-95 0.2913145134985449 96-97 0.27907079071811025 98-99 0.2976436254045769 100-101 0.3206165479652623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3136301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.674626969851424 #Duplication Level Percentage of deduplicated Percentage of total 1 89.5477942126156 42.691576850594686 2 4.534495780566094 4.323607896697077 3 1.555878286520645 2.225277507610901 4 1.0304632241487377 1.9650779926976587 5 0.539626938040064 1.2863256486971593 6 0.4234061788352503 1.2111438979620455 7 0.3271075663854562 1.0916311844509774 8 0.1853022903018226 0.7067374053438809 9 0.16495493595716199 0.7077748534734086 >10 1.3617149823551773 13.146903093170504 >50 0.1626611053254028 5.445020859485204 >100 0.14165679912386345 14.313630009032764 >500 0.01991528950534095 6.4987676649563815 >1k 0.00482006800341044 3.8371837385607845 >5k 2.0234231591374847E-4 0.5493413972664702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5883 0.18757765915962785 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5663 0.18056302631667048 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5538 0.17657743947408108 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4927 0.15709589098750407 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4424 0.1410578895329243 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3886 0.12390392376241947 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3405 0.10856738559213545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3208 0.10228610072821455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0056435909691066 0.0 0.0 2 0.005962437916513753 0.0 0.0 3 0.006600131811328058 0.0 0.0 4 0.007046517537698071 0.0 0.0 5 0.008226251243104537 0.0 0.0 6 0.009182792085325993 0.0 0.0 7 0.01042629518021389 0.0 0.0 8 0.012052414611990367 0.0 0.0 9 0.0135828799595447 0.0 0.0 10-11 0.021920727634241736 0.0 0.0 12-13 0.02950928498253197 0.0 0.0 14-15 0.03800655613093258 0.0 0.0 16-17 0.04688644361622178 0.0 0.0 18-19 0.056435909691066 0.0 0.0 20-21 0.0645983915446891 0.0 0.0 22-23 0.07307972034571937 0.0 0.0 24-25 0.08414370942074756 0.0 0.0 26-27 0.0957178536116272 0.0 0.0 28-29 0.10995436981335657 0.0 0.0 30-31 0.2060070127197613 0.0 0.0 32-33 0.22405374994300611 0.0 0.0 34-35 0.23800330389206903 0.0 0.0 36-37 0.25161806854635443 0.0 0.0 38-39 0.26689083732715707 0.0 0.0 40-41 0.28213172141321896 0.0 0.0 42-43 0.2994610530047977 0.0 0.0 44-45 0.3176672137017461 0.0 0.0 46-47 0.3374038397462488 0.0 0.0 48-49 0.3585115076646024 0.0 0.0 50-51 0.38138877614106553 0.0 1.5942347370357628E-5 52-53 0.4058762217019348 0.0 3.1884694740715256E-5 54-55 0.43269124997887637 0.0 3.1884694740715256E-5 56-57 0.46186574566663086 0.0 3.1884694740715256E-5 58-59 0.4938301521441979 0.0 3.1884694740715256E-5 60-61 0.5291583939169104 0.0 3.1884694740715256E-5 62-63 0.5674678546478797 0.0 3.1884694740715256E-5 64-65 0.605857027115701 0.0 3.1884694740715256E-5 66-67 0.6477535160050008 0.0 3.1884694740715256E-5 68-69 0.6962820214003694 0.0 3.1884694740715256E-5 70-71 0.7446351609746642 0.0 3.1884694740715256E-5 72-73 0.7970217144336593 0.0 3.1884694740715256E-5 74-75 0.8553547634617978 0.0 3.1884694740715256E-5 76-77 0.9182792085325995 0.0 3.1884694740715256E-5 78-79 0.9902270222150233 0.0 3.1884694740715256E-5 80-81 1.0660966533505554 0.0 3.1884694740715256E-5 82-83 1.147976549444712 0.0 3.1884694740715256E-5 84-85 1.2343840721920505 0.0 3.1884694740715256E-5 86-87 1.331632391151232 0.0 3.1884694740715256E-5 88-89 1.437824366985184 0.0 3.1884694740715256E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3015 0.0 89.19223 9 CGGGAGA 3875 0.0 87.676 4 AGAGCGG 3250 0.0 86.25218 8 ATTCGGA 830 0.0 85.76462 2 GAGAGCG 3830 0.0 83.8516 7 AAGCGGC 1105 0.0 81.97895 9 CGGAAAG 1930 0.0 81.37599 5 TCGGAGA 1080 0.0 81.2807 4 TCGGGAG 4535 0.0 77.84703 3 TTCGGAG 775 0.0 75.92178 3 GTTCGGA 690 0.0 73.59184 2 GGATCGG 1895 0.0 73.14067 1 AAGGGGC 2910 0.0 72.68974 9 ATCCGGA 890 0.0 72.51768 2 GAACCGG 395 0.0 72.100685 1 CGGAGAG 1800 0.0 70.90965 5 GATCGGG 6090 0.0 70.69281 1 GATCCGA 155 0.0 70.43385 1 AAAGCGG 1385 0.0 69.86286 8 ATCGGGA 6090 0.0 69.43132 2 >>END_MODULE