FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136301
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC58830.18757765915962785No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56630.18056302631667048No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG55380.17657743947408108No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG49270.15709589098750407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44240.1410578895329243No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38860.12390392376241947No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA34050.10856738559213545No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.10228610072821455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC30150.089.192239
CGGGAGA38750.087.6764
AGAGCGG32500.086.252188
ATTCGGA8300.085.764622
GAGAGCG38300.083.85167
AAGCGGC11050.081.978959
CGGAAAG19300.081.375995
TCGGAGA10800.081.28074
TCGGGAG45350.077.847033
TTCGGAG7750.075.921783
GTTCGGA6900.073.591842
GGATCGG18950.073.140671
AAGGGGC29100.072.689749
ATCCGGA8900.072.517682
GAACCGG3950.072.1006851
CGGAGAG18000.070.909655
GATCGGG60900.070.692811
GATCCGA1550.070.433851
AAAGCGG13850.069.862868
ATCGGGA60900.069.431322
Produced by FastQC (version 0.11.2)