##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1854631 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56955911984648 33.0 33.0 33.0 27.0 33.0 2 31.33083723932146 33.0 33.0 33.0 27.0 33.0 3 31.262631218824662 33.0 33.0 33.0 27.0 33.0 4 34.85648465921253 37.0 37.0 37.0 33.0 37.0 5 34.86332806903368 37.0 37.0 37.0 33.0 37.0 6 35.13616832674532 37.0 37.0 37.0 33.0 37.0 7 34.886398965616344 37.0 37.0 37.0 33.0 37.0 8 34.95403506142192 37.0 37.0 37.0 33.0 37.0 9 34.662166220665995 37.0 37.0 37.0 33.0 37.0 10-11 34.6142227213931 37.0 37.0 37.0 33.0 37.0 12-13 34.615128292366514 37.0 37.0 37.0 33.0 37.0 14-15 36.24577584435933 40.0 37.0 40.0 33.0 40.0 16-17 36.12403895977151 40.0 37.0 40.0 30.0 40.0 18-19 36.07218929264096 40.0 37.0 40.0 27.0 40.0 20-21 36.01313145310307 40.0 37.0 40.0 27.0 40.0 22-23 36.33322019312737 40.0 37.0 40.0 27.0 40.0 24-25 35.87554963763681 40.0 37.0 40.0 27.0 40.0 26-27 35.696188621887586 37.0 37.0 40.0 27.0 40.0 28-29 35.465754104185685 37.0 37.0 40.0 27.0 40.0 30-31 35.451783400579416 37.0 37.0 40.0 27.0 40.0 32-33 35.2606367520008 37.0 37.0 40.0 27.0 40.0 34-35 35.16223820263977 37.0 37.0 40.0 27.0 40.0 36-37 34.971417764504096 37.0 37.0 40.0 22.0 40.0 38-39 34.8594572181744 37.0 37.0 40.0 22.0 40.0 40-41 34.79560866824721 37.0 37.0 40.0 22.0 40.0 42-43 34.50099157190837 37.0 33.0 40.0 22.0 40.0 44-45 34.29843429771205 37.0 33.0 40.0 22.0 40.0 46-47 34.15669397308683 37.0 33.0 40.0 22.0 40.0 48-49 33.96195038258284 37.0 33.0 40.0 22.0 40.0 50-51 32.77722522701281 35.0 33.0 38.5 18.5 38.5 52-53 33.23826896024061 37.0 33.0 37.0 22.0 40.0 54-55 33.86810206450771 37.0 33.0 40.0 22.0 40.0 56-57 33.904333260902035 37.0 33.0 40.0 22.0 40.0 58-59 33.564620940769345 37.0 33.0 38.5 22.0 40.0 60-61 33.28574066755058 37.0 33.0 37.0 22.0 40.0 62-63 33.022100083520655 37.0 33.0 37.0 15.0 40.0 64-65 32.76446716354897 37.0 33.0 37.0 15.0 40.0 66-67 32.570262763859766 37.0 33.0 37.0 15.0 38.5 68-69 32.454642999065584 37.0 33.0 37.0 15.0 37.0 70-71 32.15128373245136 37.0 33.0 37.0 15.0 37.0 72-73 32.09401061451038 37.0 33.0 37.0 18.5 37.0 74-75 32.02580944673091 37.0 33.0 37.0 22.0 37.0 76-77 31.810443425134164 33.0 33.0 37.0 22.0 37.0 78-79 31.560293934480768 33.0 33.0 37.0 15.0 37.0 80-81 31.299675784563075 33.0 33.0 37.0 15.0 37.0 82-83 30.994813523552665 33.0 33.0 37.0 15.0 37.0 84-85 30.86878009695729 33.0 33.0 37.0 15.0 37.0 86-87 30.715956165943524 33.0 30.0 37.0 15.0 37.0 88-89 30.477852198092236 33.0 27.0 37.0 15.0 37.0 90-91 30.415775968373225 33.0 27.0 37.0 15.0 37.0 92-93 30.255538972442494 33.0 27.0 37.0 15.0 37.0 94-95 30.081851592041758 33.0 27.0 37.0 15.0 37.0 96-97 29.90835832033434 33.0 27.0 37.0 15.0 37.0 98-99 29.741841368983913 33.0 27.0 37.0 15.0 37.0 100-101 28.38169695211608 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2970816452598122 2306 2 0.3616082088774242 2306 3 0.263043541380398 2306 4 0.45865563585340396 2306 5 0.4568270517135602 2306 6 0.3603714183513347 2306 7 0.7151850438894485 2306 8 0.3658256731177971 2306 9 0.5010047241781663 2306 10-11 0.4222343416989034 2306 12-13 0.3943032801071169 2306 14-15 0.8409939672166189 2306 16-17 0.6521517475283076 2306 18-19 0.8776372647835373 2306 20-21 0.6967406636611031 2306 22-23 0.589076506851292 2306 24-25 0.642748531468115 2306 26-27 0.3515098648839512 2306 28-29 0.5432082878207609 2306 30-31 0.5035385798179703 2306 32-33 0.5145649637614369 2306 34-35 0.47209374506079627 2306 36-37 0.5177935828702047 2306 38-39 0.7343717989535179 2306 40-41 0.4455808401948431 2306 42-43 0.5422552363025304 2306 44-45 0.6611035031997332 2306 46-47 0.604586231080944 2306 48-49 0.5778374253716407 2306 50-51 0.6081591513221696 2306 52-53 0.6116419469076178 2306 54-55 0.7188392708030307 2306 56-57 0.568010634785189 2306 58-59 0.6325307862263898 2306 60-61 0.6071354288428097 2306 62-63 0.602116812900853 2306 64-65 0.738952903693523 2306 66-67 0.7015320467909802 2306 68-69 0.7797992444980437 2306 70-71 0.697476540672767 2306 72-73 0.6644478641081903 2306 74-75 0.8154873513959089 2306 76-77 0.4692933479655963 2306 78-79 0.37136700159550173 2306 80-81 0.5669849845969956 2306 82-83 0.5558570857457177 2306 84-85 0.6173683681314692 2306 86-87 0.5030937631754497 2306 88-89 0.6424025928135677 2306 90-91 0.5151794761288144 2306 92-93 0.551270772936995 2306 94-95 0.5881328048954408 2306 96-97 0.6198284825211573 2306 98-99 0.3167978022934257 2306 100-101 0.2016233650011472 2307 1 0.156754127908588 2307 2 0.17318946178134453 2307 3 0.2660681413395416 2307 4 0.43525882750240186 2307 5 0.426873478328595 2307 6 0.2689988501893623 2307 7 0.5391467274689319 2307 8 0.319319058952253 2307 9 0.4334257962207744 2307 10-11 0.3978926848400448 2307 12-13 0.3153667352380012 2307 14-15 0.6061510909359527 2307 16-17 0.6146199512630446 2307 18-19 0.6156141183679509 2307 20-21 0.4679591905561864 2307 22-23 0.45351684738545117 2307 24-25 0.45721721067792487 2307 26-27 0.3808361004411722 2307 28-29 0.3533823243509886 2307 30-31 0.3858991779402956 2307 32-33 0.26879655627748633 2307 34-35 0.3043190678143546 2307 36-37 0.3382081862824151 2307 38-39 0.2833389514002249 2307 40-41 0.3042551415809527 2307 42-43 0.4121063656010122 2307 44-45 0.453629369585812 2307 46-47 0.28356417663091094 2307 48-49 0.17963971090617292 2307 50-51 0.2730439164139824 2307 52-53 0.3463523952462637 2307 54-55 0.4445805508976193 2307 56-57 0.28477257725450755 2307 58-59 0.33367753084926477 2307 60-61 0.5359000433735517 2307 62-63 0.40216485757742504 2307 64-65 0.5640754786350826 2307 66-67 0.5257323718495996 2307 68-69 0.4778400341120772 2307 70-71 0.4781977893462255 2307 72-73 0.20494731179780246 2307 74-75 0.305513448287968 2307 76-77 0.2969019976344782 2307 78-79 0.4765895890115033 2307 80-81 0.23758268206830024 2307 82-83 0.24571988497411112 2307 84-85 0.46734558871755283 2307 86-87 0.5940017036090914 2307 88-89 0.7925896642306434 2307 90-91 0.41328476221836397 2307 92-93 0.5665535450108763 2307 94-95 0.469831037321395 2307 96-97 0.359374584619939 2307 98-99 0.3771309331430821 2307 100-101 0.22121072314957857 2201 1 0.349677151860039 2201 2 0.282322432148689 2201 3 0.27265102455200463 2201 4 0.33415873927153683 2201 5 0.40881498772195357 2201 6 0.3006913763896222 2201 7 0.5946176866029731 2201 8 0.44594369042700066 2201 9 0.5056257574852765 2201 10-11 0.5117259930670386 2201 12-13 0.5242018726414344 2201 14-15 0.7401193278250631 2201 16-17 0.6773340187058565 2201 18-19 0.6648131542843672 2201 20-21 0.6272367734609148 2201 22-23 0.5425942968678967 2201 24-25 0.7883657182851422 2201 26-27 0.6465601315156562 2201 28-29 0.7079871146412842 2201 30-31 0.6040499881578612 2201 32-33 0.776146832805928 2201 34-35 0.635275404296749 2201 36-37 0.6007195379362074 2201 38-39 0.7090907429171551 2201 40-41 0.7177492989762086 2201 42-43 0.8587378833870716 2201 44-45 0.8434660348895662 2201 46-47 0.7478627412654006 2201 48-49 0.8369824555316185 2201 50-51 0.693082221520612 2201 52-53 0.8560877026555005 2201 54-55 1.0422355467012707 2201 56-57 1.061517934374315 2201 58-59 0.8567496864799082 2201 60-61 0.9240398785324686 2201 62-63 1.148361676962395 2201 64-65 1.055405827900195 2201 66-67 1.0506132601837592 2201 68-69 0.8517592060331438 2201 70-71 1.015661259616735 2201 72-73 0.9395654443162513 2201 74-75 0.9513536858657048 2201 76-77 0.9879558184612733 2201 78-79 0.9113788033264285 2201 80-81 1.0251959296096764 2201 82-83 1.003577166172331 2201 84-85 1.1611291863848159 2201 86-87 1.1502762091934606 2201 88-89 1.2158430159274758 2201 90-91 1.0904172591518062 2201 92-93 0.9842653528827938 2201 94-95 1.1024037201852401 2201 96-97 0.928857680877659 2201 98-99 0.9781165364485105 2201 100-101 0.711167468506698 1101 1 0.3425934524994787 1101 2 0.3631146853101406 1101 3 0.34738390979991607 1101 4 0.4971941434677376 1101 5 0.4787522693824542 1101 6 0.34986255455464743 1101 7 0.5393503675566222 1101 8 0.3141836953300654 1101 9 0.41027631214420524 1101 10-11 0.4404963458832327 1101 12-13 0.591770975176722 1101 14-15 0.7587863077429375 1101 16-17 0.6044937155331738 1101 18-19 0.6533046454282143 1101 20-21 0.590752473475213 1101 22-23 0.49016952823115645 1101 24-25 0.5674124812425063 1101 26-27 0.6229635309722283 1101 28-29 0.5608292564623696 1101 30-31 0.6477638536096535 1101 32-33 0.7389803942678626 1101 34-35 0.6023245779264457 1101 36-37 0.7422354013859191 1101 38-39 0.5207220326268498 1101 40-41 0.37376147491442424 1101 42-43 0.632279324478823 1101 44-45 0.8629818574804275 1101 46-47 0.6930753094486093 1101 48-49 0.6364316781919399 1101 50-51 0.6569025242847104 1101 52-53 0.6716163140673785 1101 54-55 0.7109717823751396 1101 56-57 0.7187084796357155 1101 58-59 0.7891090809516967 1101 60-61 0.8085141272403078 1101 62-63 0.8478975203912711 1101 64-65 1.0012529340719212 1101 66-67 0.8746343842108075 1101 68-69 0.7269425601363935 1101 70-71 0.890527140431459 1101 72-73 0.7712296254026896 1101 74-75 0.8019829122033215 1101 76-77 0.7959666051984868 1101 78-79 0.863796217369611 1101 80-81 0.9096144059830387 1101 82-83 1.0546247871656114 1101 84-85 1.0979403571952524 1101 86-87 0.7946628156614146 1101 88-89 1.006995848467465 1101 90-91 0.9180834014631927 1101 92-93 0.8647910020278218 1101 94-95 0.9745375737879698 1101 96-97 0.9837819898523072 1101 98-99 0.8564143566286972 1101 100-101 0.8122665716548738 2304 1 0.09885199991988003 2304 2 0.17461633895872453 2304 3 -0.038623714422591604 2304 4 0.13971195728618824 2304 5 0.22081903945952774 2304 6 0.2404085339398776 2304 7 0.2185283925403283 2304 8 0.10835837018390038 2304 9 0.19505150160246387 2304 10-11 0.1966024929891219 2304 12-13 0.25241716015896287 2304 14-15 0.4447850594753646 2304 16-17 0.37393211750592315 2304 18-19 0.38148137931163717 2304 20-21 0.24901914840247485 2304 22-23 0.2794853674616391 2304 24-25 0.45825312673145646 2304 26-27 0.42404727504066386 2304 28-29 0.3919180792665031 2304 30-31 0.40294355212856203 2304 32-33 0.2785790441196312 2304 34-35 0.4678151424921637 2304 36-37 0.3895975183008673 2304 38-39 0.4390377586565961 2304 40-41 0.3239020962334891 2304 42-43 0.5161638612583488 2304 44-45 0.6567144375699456 2304 46-47 0.43745561955743284 2304 48-49 0.44246126182739687 2304 50-51 0.33441695737848676 2304 52-53 0.5843649350070663 2304 54-55 0.5701707189001226 2304 56-57 0.548495493981612 2304 58-59 0.6883072679042428 2304 60-61 0.5939755961575273 2304 62-63 0.3847770579815588 2304 64-65 0.5893932830973228 2304 66-67 0.6826870485583854 2304 68-69 0.6147306101160481 2304 70-71 0.5137101921397189 2304 72-73 0.5397645248835019 2304 74-75 0.6002316662303642 2304 76-77 0.5463744720377051 2304 78-79 0.5186879041523582 2304 80-81 0.3787411999852388 2304 82-83 0.6186663823169489 2304 84-85 0.7314030682610806 2304 86-87 0.488306441389188 2304 88-89 0.655017474397166 2304 90-91 0.6976718173565715 2304 92-93 0.5110769470856908 2304 94-95 0.5310234022796756 2304 96-97 0.6546906246208408 2304 98-99 0.8736420935624274 2304 100-101 0.647894132056642 2202 1 0.24851510696284862 2202 2 0.2351426547481985 2202 3 0.24092116226186988 2202 4 0.2615082959589543 2202 5 0.26245392377175136 2202 6 0.2517421215320468 2202 7 0.38220116141904725 2202 8 0.179582626476801 2202 9 0.24617659694952465 2202 10-11 0.3095792283056724 2202 12-13 0.3186293391571766 2202 14-15 0.5185328347105553 2202 16-17 0.46972413377849165 2202 18-19 0.5026230014830091 2202 20-21 0.48442786948053396 2202 22-23 0.38469767358927953 2202 24-25 0.6974375915166107 2202 26-27 0.694292634816918 2202 28-29 0.7450518194139732 2202 30-31 0.7359590619175265 2202 32-33 0.6142547369749565 2202 34-35 0.5397481452890105 2202 36-37 0.5632435900018535 2202 38-39 0.5611582773446173 2202 40-41 0.6585925361012741 2202 42-43 0.7548461648433928 2202 44-45 0.8316890116863931 2202 46-47 0.5960781723161475 2202 48-49 0.755622334799682 2202 50-51 0.8062514348765788 2202 52-53 0.8038220923932826 2202 54-55 0.8899774821851452 2202 56-57 0.8694654914390227 2202 58-59 0.7828089205866817 2202 60-61 0.9192521026411313 2202 62-63 0.9932343424971961 2202 64-65 1.0630043942084342 2202 66-67 0.9157311624663507 2202 68-69 0.9981561122772504 2202 70-71 0.9917676546365399 2202 72-73 0.8888523713111667 2202 74-75 0.8601350478728733 2202 76-77 0.9078256543412948 2202 78-79 0.9620994220775998 2202 80-81 0.8925983065196839 2202 82-83 0.9774160644934007 2202 84-85 1.097627960201077 2202 86-87 0.9916118062506172 2202 88-89 1.1163542402247764 2202 90-91 1.0944014130850235 2202 92-93 1.0667214932336684 2202 94-95 0.9750070025847855 2202 96-97 0.9100046342826786 2202 98-99 0.9511687907401516 2202 100-101 1.1653553575842324 1102 1 0.16561056670062868 1102 2 0.08998724922759394 1102 3 0.1058777714719703 1102 4 0.07447097476481446 1102 5 0.20137776398453155 1102 6 0.14733484483690518 1102 7 0.2588366276356098 1102 8 0.1336668165438084 1102 9 0.1414181020728762 1102 10-11 0.21146830473824707 1102 12-13 0.18463448808392258 1102 14-15 0.09395267683287045 1102 16-17 0.06927232398188465 1102 18-19 0.30513949680294417 1102 20-21 0.17694146639799868 1102 22-23 0.23810073907612406 1102 24-25 0.25590263166801464 1102 26-27 0.41221328678697944 1102 28-29 0.3334164384445444 1102 30-31 0.4582068582791763 1102 32-33 0.4140646358718598 1102 34-35 0.4701131735058155 1102 36-37 0.3591056942420465 1102 38-39 0.25851987862673553 1102 40-41 0.2759906231556428 1102 42-43 0.378324077311639 1102 44-45 0.3767736620879063 1102 46-47 0.42450809064939676 1102 48-49 0.35998443051516205 1102 50-51 0.30838176069111967 1102 52-53 0.29124899297808327 1102 54-55 0.44959847343879034 1102 56-57 0.45730839802521217 1102 58-59 0.5214275760689233 1102 60-61 0.5370286869590686 1102 62-63 0.6456587709151833 1102 64-65 0.6037073420542427 1102 66-67 0.5702944414120097 1102 68-69 0.4327664476394659 1102 70-71 0.528683924904044 1102 72-73 0.6236536602477898 1102 74-75 0.7002063144747481 1102 76-77 0.6104367739771135 1102 78-79 0.46173712176900494 1102 80-81 0.5534852176751279 1102 82-83 0.5395416164685791 1102 84-85 0.4994241172603928 1102 86-87 0.46530113160482145 1102 88-89 0.6531307051967339 1102 90-91 0.6920358051877287 1102 92-93 0.5456356152734614 1102 94-95 0.45470880811903314 1102 96-97 0.6690749290672571 1102 98-99 0.5509087869892788 1102 100-101 0.35392115984498673 2305 1 0.23239354362766562 2305 2 0.24921412679180577 2305 3 0.25949965978773903 2305 4 0.44990169650405676 2305 5 0.39387410317954874 2305 6 0.39547607935269014 2305 7 0.2095687886318771 2305 8 0.3766136656131067 2305 9 0.23708977733638648 2305 10-11 0.3535731686618391 2305 12-13 0.4709519821144923 2305 14-15 0.45761786818412986 2305 16-17 0.4174117445852943 2305 18-19 0.43478542110673146 2305 20-21 0.28791514158236 2305 22-23 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0.5624690559216354 2305 90-91 0.5440838771637715 2305 92-93 0.5053060768646915 2305 94-95 0.6388009614638861 2305 96-97 0.3181180078372279 2305 98-99 0.3254490426519858 2305 100-101 0.3276449900364007 2203 1 0.24594423769929463 2203 2 0.046732628977554924 2203 3 0.052605917936368485 2203 4 -0.09356406007236728 2203 5 0.10485654048302706 2203 6 0.288139435438282 2203 7 0.12272115844551479 2203 8 0.09560812454400747 2203 9 0.10055946442896158 2203 10-11 0.044767614171462355 2203 12-13 0.051456861706491 2203 14-15 0.023873428426483656 2203 16-17 -0.04859408408255206 2203 18-19 0.17881055723680817 2203 20-21 0.1530852562383842 2203 22-23 0.1975529491368988 2203 24-25 0.2598976346328712 2203 26-27 0.32926481986994816 2203 28-29 0.013703011306134272 2203 30-31 0.157101519049057 2203 32-33 0.39481277047678276 2203 34-35 0.37836384060073414 2203 36-37 0.28901949693019446 2203 38-39 0.2921515001615518 2203 40-41 0.30648597202158356 2203 42-43 0.12625531094311526 2203 44-45 0.20033215025315343 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-0.9463460689272694 1213 76-77 -1.0285020199053747 1213 78-79 -1.1519293317026076 1213 80-81 -1.30720061970489 1213 82-83 -1.5406562760007354 1213 84-85 -1.6759317596315242 1213 86-87 -1.4737530631896227 1213 88-89 -1.4289086643123952 1213 90-91 -1.475844159491551 1213 92-93 -1.7093738751597414 1213 94-95 -1.534654557775351 1213 96-97 -1.5980059560634778 1213 98-99 -1.700162948743781 1213 100-101 -1.5524783661500763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1345.0 3 184.0 4 180.0 5 168.0 6 263.0 7 2234.0 8 13226.0 9 22228.0 10 20316.0 11 14521.0 12 10035.0 13 8433.0 14 8612.0 15 9662.0 16 9638.0 17 8655.0 18 8637.0 19 8714.0 20 9252.0 21 9974.0 22 10379.0 23 11119.0 24 12446.0 25 13946.0 26 16311.0 27 18946.0 28 22643.0 29 27038.0 30 34829.0 31 45524.0 32 62340.0 33 89890.0 34 138572.0 35 238915.0 36 428485.0 37 453074.0 38 63867.0 39 30.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.453646654868095 22.558761301705673 24.558607783379156 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27.17120267763477 20.74696978045426 20-21 25.837918925700578 26.41441654600029 27.232922162827627 20.51474236547151 22-23 28.06698651667251 25.557624707346427 25.559190746544385 20.816198029436677 24-25 26.26635939633242 26.8454027398121 25.741916210965694 21.146321652889785 26-27 24.02158047090712 28.166852697437157 26.130512192701012 21.68105463895471 28-29 24.787902379948314 27.489105870394997 25.36894064726809 22.3540511023886 30-31 26.15945823420055 25.919079532315827 26.16746004433976 21.75400218914387 32-33 24.907866884787833 25.386361405210188 27.826107416110656 21.879664293891317 34-35 23.828286944925896 26.3021580554757 26.63801302800342 23.231541971594982 36-37 24.767660706752768 27.66197769629317 26.185033152697105 21.385328444256956 38-39 25.639978393975632 26.843050950240414 24.77162610758599 22.74534454819796 40-41 25.21870191107371 25.965890048680972 24.23420857376951 24.581199466475805 42-43 26.790275655151813 25.562474835156745 25.31602172510683 22.331227784584613 44-45 24.976601764740888 26.47249287232949 25.571566139913333 22.97933922301629 46-47 25.679924002087724 26.75149240061463 25.19722578398551 22.371357813312134 48-49 24.154134473940054 26.494165409254517 25.942436943192742 23.409263173612686 50-51 23.54120670995671 25.709442640692643 25.804653679653676 24.94469696969697 52-53 26.382975845384486 25.454476591381603 24.36027534936066 23.802272213873245 54-55 26.8828462350919 25.282325846206692 25.06537993933304 22.769447979368366 56-57 27.184777829636698 25.14507193733639 24.68553880287073 22.984611430156185 58-59 26.08398299855183 25.083033694216873 25.077217209975895 23.755766097255403 60-61 24.437359471708277 25.31021020355068 24.881641170320425 25.370789154420613 62-63 26.090813596488267 25.03361538804717 25.244135726945437 23.631435288519125 64-65 25.271164602382502 26.212429509912216 25.828406406874667 22.687999480830616 66-67 24.206727265401202 26.087455045151582 25.20684165536063 24.498976034086585 68-69 23.51303394919912 24.503321467119463 28.336902753208626 23.646741830472795 70-71 23.525787082813864 26.587650828079173 26.726490935917518 23.160071153189442 72-73 23.65432122801097 28.45578182730663 25.25482612119167 22.63507082349073 74-75 24.030418333199936 28.4648930210021 25.202336427370874 22.30235221842708 76-77 24.035766859951348 27.967357927105606 24.916769690539002 23.080105522404043 78-79 23.97054150999774 28.208978430728337 25.059807981698633 22.760672077575286 80-81 24.275867366174275 28.67031890558324 24.816319752651687 22.237493975590798 82-83 23.7827880492852 28.24810311481396 25.1552267470955 22.813882088805336 84-85 23.49730825855973 28.339656845229804 25.05173720986657 23.111297686343892 86-87 23.25956882793692 28.528577997781923 25.042765560334335 23.16908761394682 88-89 23.42724945077951 28.598685765817034 25.138393825576987 22.83567095782647 90-91 23.797079643768242 27.910283333720926 24.85842508008292 23.43421194242791 92-93 23.436603968151186 27.693432026559243 24.558596376628014 24.311367628661557 94-95 23.581614219185127 28.273211995551435 24.00508237498009 24.140091410283347 96-97 23.433934993063804 28.817510306963527 23.763968710197304 23.98458598977536 98-99 23.633732234287024 27.63035851329126 24.39868750463089 24.33722174779083 100-101 24.405209322554917 27.8345889431861 23.910583880896077 23.849617853362908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3003.0 1 1882.0 2 699.0 3 1043.0 4 1229.0 5 958.5 6 1288.5 7 1823.5 8 1998.0 9 1933.0 10 1733.5 11 1550.5 12 1420.0 13 1299.0 14 1197.0 15 1129.0 16 1074.5 17 1040.0 18 996.0 19 986.0 20 994.0 21 1008.5 22 1100.5 23 1147.5 24 1219.5 25 1430.5 26 1715.5 27 2073.5 28 2520.5 29 3097.5 30 3893.0 31 4915.0 32 6309.5 33 8020.0 34 10581.5 35 15375.5 36 21234.0 37 28628.0 38 36521.0 39 41015.0 40 46405.5 41 50389.0 42 56792.0 43 69619.0 44 76751.0 45 82457.0 46 94974.0 47 102045.5 48 99252.0 49 96077.5 50 93230.0 51 91083.0 52 88453.5 53 85379.0 54 80254.0 55 70630.0 56 61389.0 57 54841.5 58 49906.5 59 42426.5 60 32391.5 61 23680.5 62 17044.0 63 12769.0 64 10221.0 65 8423.5 66 7668.5 67 7034.0 68 5869.5 69 4432.5 70 3028.0 71 2169.5 72 1867.0 73 1587.0 74 1087.5 75 703.0 76 519.0 77 428.5 78 362.5 79 299.0 80 247.5 81 201.0 82 152.0 83 110.0 84 87.5 85 66.5 86 54.0 87 43.5 88 33.5 89 28.0 90 23.5 91 17.0 92 11.5 93 9.5 94 6.5 95 4.5 96 4.0 97 3.5 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25282657304876277 2 0.23988599349412362 3 0.20408372339295525 4 0.20839724991116834 5 0.2226318874212714 6 0.2264601422061855 7 0.20127993115611675 8 0.19745167637120267 9 0.1791191886687972 10-11 0.16685259763262883 12-13 0.19556450851948448 14-15 0.192760716282646 16-17 0.1928685544456013 18-19 0.18575123569054977 20-21 0.18615562880163225 22-23 0.1523483647151374 24-25 0.45736860863427825 26-27 0.31933576005146036 28-29 0.2701885172845704 30-31 0.2722644019214604 32-33 0.31335074200743973 34-35 0.3109783024224226 36-37 0.30253996617116824 38-39 0.32742362227310984 40-41 0.3325459350134879 42-43 0.3674585402702748 44-45 0.3343522242429896 46-47 0.3604760192189174 48-49 0.33742561188721637 50-51 0.3575374292783848 52-53 0.3468884106865463 54-55 0.32424239646592773 56-57 0.3595593948337971 58-59 0.34699624884950164 60-61 0.31294634889635725 62-63 0.29135175676455316 64-65 0.2978220465418728 66-67 0.30693437131159784 68-69 0.3140516900666494 70-71 0.29016553697204456 72-73 0.29801076332704457 74-75 0.29563832374202736 76-77 0.29898130679364254 78-79 0.30132678683792086 80-81 0.31823041888116826 82-83 0.33462181965037785 84-85 0.32925687104335044 86-87 0.3332738426134363 88-89 0.3290142351767009 90-91 0.3024860470896906 92-93 0.28746958289816144 94-95 0.2963123122604982 96-97 0.2843961952539346 98-99 0.3037801050451545 100-101 0.3267765932953779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1854631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.72718757916663 #Duplication Level Percentage of deduplicated Percentage of total 1 83.86815096786371 43.38263577032552 2 7.089696998709876 7.334601730634407 3 2.9749826211015407 4.616624522594407 4 1.5116827488844613 3.127803884469471 5 0.9942223452508675 2.5714162874095297 6 0.5877452212846097 1.824144438608868 7 0.43652979804384934 1.5806321123116946 8 0.29141709568632984 1.2059349417874188 9 0.2570873083877875 1.196856308267833 >10 1.7551885525945794 17.581240379341434 >50 0.15200537182441318 5.295325452505298 >100 0.07654217268188178 7.216439549708747 >500 0.0036061303861205356 1.2534773646798372 >1k 9.352500564972662E-4 1.1293156175230976 >5k 2.07417243371655E-4 0.6835516398325506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6437 0.3470771274717181 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.33995980871666653 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4691 0.2529344112117181 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4030 0.21729389835498275 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 3912 0.21093144674061848 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0029655494812714767 0.0 0.0 2 0.0031273067257044664 0.0 0.0 3 0.003342983051615119 0.0 0.0 4 0.0038821738663917514 0.0 0.0 5 0.004798798251512026 0.0 0.0 6 0.005499746310721648 0.0 0.0 7 0.006254613451408933 0.0 0.0 8 0.00722515691800687 0.0 0.0 9 0.008087862221649481 0.0 0.0 10-11 0.01582525041369415 0.0 0.0 12-13 0.02701345982030927 0.0 0.0 14-15 0.03863302187874569 0.0 0.0 16-17 0.049902109907577305 0.0 0.0 18-19 0.060065856766116824 0.0 0.0 20-21 0.0650803313435395 0.0 0.0 22-23 0.06971737235061853 0.0 0.0 24-25 0.07678077202419242 0.0 0.0 26-27 0.08405984802367694 0.0 0.0 28-29 0.09193203391941578 0.0 0.0 30-31 0.14684861840441576 0.0 0.0 32-33 0.1569584461814776 0.0 0.0 34-35 0.16528894426977658 0.0 0.0 36-37 0.17326896832847072 0.0 0.0 38-39 0.1810872351427319 0.0 0.0 40-41 0.18887854241625424 0.0 0.0 42-43 0.1974786359119415 0.0 0.0 44-45 0.207480625526048 0.0 0.0 46-47 0.2165659907550343 0.0 0.0 48-49 0.22786203832460472 0.0 0.0 50-51 0.23953551946451884 0.0 0.0 52-53 0.2514516364710824 0.0 0.0 54-55 0.26458093281089334 0.0 0.0 56-57 0.2794356397579896 0.0 0.0 58-59 0.296339271801237 0.0 0.0 60-61 0.3156962220517181 0.0 0.0 62-63 0.335565403576237 0.0 0.0 64-65 0.35702519800434696 0.0 0.0 66-67 0.3811539869656012 0.0 0.0 68-69 0.40681946974896893 0.0 0.0 70-71 0.4337520509470617 0.0 0.0 72-73 0.46604958075218195 0.0 0.0 74-75 0.5044130072235393 0.0 0.0 76-77 0.5489501685240892 0.0 0.0 78-79 0.5992836310834877 0.0 0.0 80-81 0.6527983194500685 0.0 0.0 82-83 0.710356938927474 0.0 0.0 84-85 0.7782141029671131 0.0 0.0 86-87 0.8502230362805323 0.0 0.0 88-89 0.9313442943636767 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1205 0.0 66.14861 9 CGGGAGA 1750 0.0 59.404003 4 TCGGGAG 1975 0.0 55.280304 3 ATCGGGA 2235 0.0 55.022144 2 GGAGAGC 1685 0.0 54.35922 6 AGAGCGG 1485 0.0 53.995644 8 ATTGGGA 1015 0.0 51.924488 2 CGGAAAG 610 0.0 47.46639 5 GATTGGG 1190 0.0 46.288517 1 GATCGGG 2855 0.0 44.574955 1 AGAGCGT 3650 0.0 43.41623 8 AAGCGGC 370 0.0 42.316593 9 TTGGGAG 1075 0.0 40.624596 3 TGTGTTT 41460 0.0 40.510536 1 GAGCGTC 3945 0.0 39.448032 9 TTGTGTT 23155 0.0 38.65706 1 AAGAGCG 3925 0.0 37.958755 7 CGGAAGA 4100 0.0 37.396275 4 ATCGGAA 4545 0.0 36.668106 2 TCGGAAG 4525 0.0 35.037857 3 >>END_MODULE