FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854631
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64370.3470771274717181No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.33995980871666653No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA46910.2529344112117181No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40300.21729389835498275No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT39120.21093144674061848Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC12050.066.148619
CGGGAGA17500.059.4040034
TCGGGAG19750.055.2803043
ATCGGGA22350.055.0221442
GGAGAGC16850.054.359226
AGAGCGG14850.053.9956448
ATTGGGA10150.051.9244882
CGGAAAG6100.047.466395
GATTGGG11900.046.2885171
GATCGGG28550.044.5749551
AGAGCGT36500.043.416238
AAGCGGC3700.042.3165939
TTGGGAG10750.040.6245963
TGTGTTT414600.040.5105361
GAGCGTC39450.039.4480329
TTGTGTT231550.038.657061
AAGAGCG39250.037.9587557
CGGAAGA41000.037.3962754
ATCGGAA45450.036.6681062
TCGGAAG45250.035.0378573
Produced by FastQC (version 0.11.2)