##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7707756 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81190102540869 33.0 33.0 33.0 27.0 33.0 2 31.667872984043605 33.0 33.0 33.0 27.0 33.0 3 31.62090367157445 33.0 33.0 33.0 27.0 33.0 4 35.257874016769605 37.0 37.0 37.0 33.0 37.0 5 35.24685784033641 37.0 37.0 37.0 33.0 37.0 6 35.42317024565905 37.0 37.0 37.0 33.0 37.0 7 35.23315268412752 37.0 37.0 37.0 33.0 37.0 8 35.31757777490621 37.0 37.0 37.0 33.0 37.0 9 35.075628496802445 37.0 37.0 37.0 33.0 37.0 10-11 35.03426859905788 37.0 37.0 37.0 33.0 37.0 12-13 35.04255187891262 37.0 37.0 37.0 33.0 37.0 14-15 36.75686198421434 40.0 37.0 40.0 33.0 40.0 16-17 36.696605925252435 40.0 37.0 40.0 33.0 40.0 18-19 36.70201508714079 40.0 37.0 40.0 33.0 40.0 20-21 36.67336660631187 40.0 37.0 40.0 33.0 40.0 22-23 36.89184069916069 40.0 37.0 40.0 33.0 40.0 24-25 36.56188584589341 40.0 37.0 40.0 33.0 40.0 26-27 36.463434039686774 40.0 37.0 40.0 33.0 40.0 28-29 36.28606691753086 40.0 37.0 40.0 33.0 40.0 30-31 36.29960438550468 40.0 37.0 40.0 33.0 40.0 32-33 36.173047122923975 40.0 37.0 40.0 33.0 40.0 34-35 36.14291208751289 40.0 37.0 40.0 33.0 40.0 36-37 36.01018863337137 40.0 37.0 40.0 27.0 40.0 38-39 35.90719944948958 40.0 37.0 40.0 27.0 40.0 40-41 35.87196617277454 37.0 37.0 40.0 27.0 40.0 42-43 35.72244554705676 37.0 37.0 40.0 27.0 40.0 44-45 35.57885187335977 37.0 37.0 40.0 27.0 40.0 46-47 35.48868743380045 37.0 37.0 40.0 27.0 40.0 48-49 35.36890711122666 37.0 37.0 40.0 27.0 40.0 50-51 34.25545846287817 37.0 35.0 38.5 24.5 38.5 52-53 34.68279036077426 37.0 35.0 38.5 27.0 40.0 54-55 35.386899312847994 37.0 37.0 40.0 27.0 40.0 56-57 35.482895345934665 37.0 37.0 40.0 27.0 40.0 58-59 35.20818847924091 37.0 37.0 40.0 27.0 40.0 60-61 34.98501308292582 37.0 37.0 40.0 27.0 40.0 62-63 34.712843854942996 37.0 33.0 40.0 27.0 40.0 64-65 34.47902359649164 37.0 33.0 40.0 27.0 40.0 66-67 34.26033939060863 37.0 33.0 37.0 27.0 40.0 68-69 34.11568924859583 37.0 33.0 37.0 27.0 40.0 70-71 33.7695241650099 37.0 33.0 37.0 27.0 40.0 72-73 33.67366533138828 37.0 33.0 37.0 27.0 38.5 74-75 33.51082772988663 37.0 33.0 37.0 27.0 37.0 76-77 33.23615504953712 37.0 33.0 37.0 27.0 37.0 78-79 32.92783528694992 37.0 33.0 37.0 22.0 37.0 80-81 32.64684312788313 37.0 33.0 37.0 22.0 37.0 82-83 32.36601476486801 37.0 33.0 37.0 22.0 37.0 84-85 32.21073318875169 37.0 33.0 37.0 22.0 37.0 86-87 32.049710966460275 33.0 33.0 37.0 22.0 37.0 88-89 31.846500914144144 33.0 33.0 37.0 22.0 37.0 90-91 31.781759373285816 33.0 33.0 37.0 22.0 37.0 92-93 31.61647598860161 33.0 33.0 37.0 15.0 37.0 94-95 31.480539926276855 33.0 33.0 37.0 15.0 37.0 96-97 31.332487107791167 33.0 33.0 37.0 15.0 37.0 98-99 31.187954055629163 33.0 33.0 37.0 15.0 37.0 100-101 29.944267371722717 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.3247741654425198 2306 2 0.3243081988207983 2306 3 0.2936360235423727 2306 4 0.42527977122148997 2306 5 0.46271576016237503 2306 6 0.40417818561221 2306 7 0.5294567949444939 2306 8 0.33702192595048786 2306 9 0.5302525822633299 2306 10-11 0.49666720524947294 2306 12-13 0.4721127538442502 2306 14-15 0.6861097950983179 2306 16-17 0.6155465437539931 2306 18-19 0.6308490854928337 2306 20-21 0.6072890821835628 2306 22-23 0.4930766609006909 2306 24-25 0.5859718665433249 2306 26-27 0.6063501074802673 2306 28-29 0.6582990783947835 2306 30-31 0.6037429707830242 2306 32-33 0.5768122281785679 2306 34-35 0.6194974477285058 2306 36-37 0.5705270676909748 2306 38-39 0.6300144680164479 2306 40-41 0.5248750423619413 2306 42-43 0.5838749991935686 2306 44-45 0.6152843407979773 2306 46-47 0.6643891928132248 2306 48-49 0.667132548462142 2306 50-51 0.6880327855983737 2306 52-53 0.6496659727366136 2306 54-55 0.6587185324358487 2306 56-57 0.6783983996513001 2306 58-59 0.7432613655743623 2306 60-61 0.7142706229460956 2306 62-63 0.5980828325073517 2306 64-65 0.7415907737499694 2306 66-67 0.7330773342664969 2306 68-69 0.7050758431923256 2306 70-71 0.7974742417366656 2306 72-73 0.5347872993581504 2306 74-75 0.6673806053282334 2306 76-77 0.5915504077862792 2306 78-79 0.6554088187029308 2306 80-81 0.6715009579205997 2306 82-83 0.7461551644790632 2306 84-85 0.6526467404984757 2306 86-87 0.7474640539726707 2306 88-89 0.8108418559522477 2306 90-91 0.6581809532939431 2306 92-93 0.5092177885448841 2306 94-95 0.4715518364152551 2306 96-97 0.477698578938881 2306 98-99 0.5570827986231954 2306 100-101 0.4972234672802536 2307 1 0.23193701634293973 2307 2 0.22780223675874467 2307 3 0.17391509850450504 2307 4 0.26006528623442904 2307 5 0.30189152540142317 2307 6 0.24531051370662027 2307 7 0.4457519234147753 2307 8 0.2340692983585697 2307 9 0.3409030225747358 2307 10-11 0.34387341418374007 2307 12-13 0.21161081247199576 2307 14-15 0.4299923867014712 2307 16-17 0.41028323278109013 2307 18-19 0.3938267460004923 2307 20-21 0.3468768218718239 2307 22-23 0.31795971486987185 2307 24-25 0.4469617686154592 2307 26-27 0.45009044923224906 2307 28-29 0.37733328517112597 2307 30-31 0.35482918193770985 2307 32-33 0.38521818222982773 2307 34-35 0.38265661432620135 2307 36-37 0.31798157815304506 2307 38-39 0.18248446990114786 2307 40-41 0.2208994922510641 2307 42-43 0.18831490972524279 2307 44-45 0.23109493227082822 2307 46-47 0.14143050892432996 2307 48-49 0.22893998414556904 2307 50-51 0.22743511351660572 2307 52-53 0.28088324189181435 2307 54-55 0.23794367211792888 2307 56-57 0.24899630773586523 2307 58-59 0.3021422336923365 2307 60-61 0.32894740382813836 2307 62-63 0.29296034207293786 2307 64-65 0.29710489585780664 2307 66-67 0.3741051230965198 2307 68-69 0.31995347574528665 2307 70-71 0.1874650143599439 2307 72-73 0.06794999602575302 2307 74-75 0.3297137122983216 2307 76-77 0.3463481118447689 2307 78-79 0.39443774699431344 2307 80-81 0.1908603665707389 2307 82-83 0.24936382185918404 2307 84-85 0.3151061754413149 2307 86-87 0.3213382292509479 2307 88-89 0.44605325628312187 2307 90-91 0.28225375317454393 2307 92-93 0.38991048332779243 2307 94-95 0.28768817821909565 2307 96-97 0.30924736553551924 2307 98-99 0.37706550393980365 2307 100-101 0.2492329479444777 2201 1 0.3225073016154454 2201 2 0.21755898125108075 2201 3 0.22598980625717502 2201 4 0.400594486521058 2201 5 0.34990650484365204 2201 6 0.30739871273579666 2201 7 0.45299382062744087 2201 8 0.24230478301320346 2201 9 0.4411245361951188 2201 10-11 0.3969208689467152 2201 12-13 0.35886517727428213 2201 14-15 0.6177844163058879 2201 16-17 0.49915393807079766 2201 18-19 0.4737316347929479 2201 20-21 0.42737155294010876 2201 22-23 0.43540026845529667 2201 24-25 0.46650868785267363 2201 26-27 0.4898695009314906 2201 28-29 0.3958663112965297 2201 30-31 0.36997844923764234 2201 32-33 0.5424563055520082 2201 34-35 0.46980228497098864 2201 36-37 0.383317248140834 2201 38-39 0.3972449653656014 2201 40-41 0.44727959788944816 2201 42-43 0.42418107870691557 2201 44-45 0.5093124642075608 2201 46-47 0.39581856570686824 2201 48-49 0.4533693092608786 2201 50-51 0.45850865548852937 2201 52-53 0.46070006305370725 2201 54-55 0.586712665711481 2201 56-57 0.5971803330255128 2201 58-59 0.5673877886757808 2201 60-61 0.5859551823206246 2201 62-63 0.5845018464648675 2201 64-65 0.5700499972104538 2201 66-67 0.5723956882175187 2201 68-69 0.5241752368839983 2201 70-71 0.5720652340706565 2201 72-73 0.44304936240940407 2201 74-75 0.5028420688836093 2201 76-77 0.442062423751473 2201 78-79 0.4510329796691721 2201 80-81 0.6155400213285063 2201 82-83 0.5326848607638652 2201 84-85 0.544265820176129 2201 86-87 0.6569990038610669 2201 88-89 0.7061915151292197 2201 90-91 0.6611214485192107 2201 92-93 0.6267300409422667 2201 94-95 0.5626929223202914 2201 96-97 0.4764902100405344 2201 98-99 0.5208629086643484 2201 100-101 0.4296218114534014 1101 1 0.17895760381215098 1101 2 0.19719104264853016 1101 3 0.1740003019551608 1101 4 0.2554853316828627 1101 5 0.2660534530321925 1101 6 0.2692626319854128 1101 7 0.29335119619793915 1101 8 0.24293281348467133 1101 9 0.2288855311120983 1101 10-11 0.25887382347447385 1101 12-13 0.3434497856881009 1101 14-15 0.43224811096832383 1101 16-17 0.4458157043135671 1101 18-19 0.3802244907183834 1101 20-21 0.3659709762518659 1101 22-23 0.4316700031239975 1101 24-25 0.45121024712734936 1101 26-27 0.4524069853166566 1101 28-29 0.46894379387208573 1101 30-31 0.36409310092060565 1101 32-33 0.5381756262915331 1101 34-35 0.3723184889847957 1101 36-37 0.3959613237597992 1101 38-39 0.3075194428777692 1101 40-41 0.31748960303067975 1101 42-43 0.39525815026409106 1101 44-45 0.4944136511809418 1101 46-47 0.3532258954411063 1101 48-49 0.38031035758218934 1101 50-51 0.38338099079535937 1101 52-53 0.4505669029185668 1101 54-55 0.361694019084176 1101 56-57 0.4485250154226321 1101 58-59 0.42603939589091055 1101 60-61 0.517776547841514 1101 62-63 0.5014728794887162 1101 64-65 0.48414329838241343 1101 66-67 0.5078098453457827 1101 68-69 0.46111233634272963 1101 70-71 0.505833031131381 1101 72-73 0.4161521654004403 1101 74-75 0.5499553381139748 1101 76-77 0.5394624309057079 1101 78-79 0.4963806794742567 1101 80-81 0.5870100432892329 1101 82-83 0.643585250529938 1101 84-85 0.6027284150487802 1101 86-87 0.6273200961880008 1101 88-89 0.7059612045578447 1101 90-91 0.5738141870308624 1101 92-93 0.5575130449816079 1101 94-95 0.6272357976393401 1101 96-97 0.5060154697852113 1101 98-99 0.45919155082374985 1101 100-101 0.3920756893697188 2304 1 0.19211514200129187 2304 2 0.1640842836280676 2304 3 0.15794352141307755 2304 4 0.30266204282856535 2304 5 0.25684601073683666 2304 6 0.24737309396633833 2304 7 0.38957259901172137 2304 8 0.1742151186564982 2304 9 0.25320881753651037 2304 10-11 0.28353556305499694 2304 12-13 0.28861677410407793 2304 14-15 0.3797493371676737 2304 16-17 0.35175251921594963 2304 18-19 0.36240510506709 2304 20-21 0.3823978365680816 2304 22-23 0.318438772116032 2304 24-25 0.45389221232448307 2304 26-27 0.3892233252711961 2304 28-29 0.36954738902216633 2304 30-31 0.3310873664270062 2304 32-33 0.36236493595014707 2304 34-35 0.3246675057875379 2304 36-37 0.3773569084549351 2304 38-39 0.33337097175759567 2304 40-41 0.3600650610753249 2304 42-43 0.2900364175208239 2304 44-45 0.3150916683685736 2304 46-47 0.24879406483520228 2304 48-49 0.30632730156188615 2304 50-51 0.2486197169301434 2304 52-53 0.3761771408034065 2304 54-55 0.4403547740413174 2304 56-57 0.4113558516652702 2304 58-59 0.534976276667237 2304 60-61 0.47229441786029014 2304 62-63 0.4028695954142876 2304 64-65 0.47323724208352047 2304 66-67 0.5017624823098359 2304 68-69 0.44781362334253316 2304 70-71 0.5154853197471922 2304 72-73 0.4080797955563824 2304 74-75 0.4454876445007443 2304 76-77 0.4941114508863862 2304 78-79 0.499699273480509 2304 80-81 0.39175410978075575 2304 82-83 0.537435799417139 2304 84-85 0.5168278107157604 2304 86-87 0.4381898289471522 2304 88-89 0.4597140157456394 2304 90-91 0.5762778573210028 2304 92-93 0.4726614876810835 2304 94-95 0.48210394453858996 2304 96-97 0.552888013267804 2304 98-99 0.3923438592984354 2304 100-101 0.4910251106216954 2202 1 0.25968142992616094 2202 2 0.21815143225389733 2202 3 0.21109453418688062 2202 4 0.25893619101874776 2202 5 0.32512565217316336 2202 6 0.31414435924054374 2202 7 0.3434414937163339 2202 8 0.28218278676201436 2202 9 0.34686533632595484 2202 10-11 0.34728271790287835 2202 12-13 0.3684681144860775 2202 14-15 0.4622058860106151 2202 16-17 0.39273289512295406 2202 18-19 0.40674867823654637 2202 20-21 0.47451789094434105 2202 22-23 0.3592647437551406 2202 24-25 0.5375449915758068 2202 26-27 0.6179236939457127 2202 28-29 0.5435036752677647 2202 30-31 0.5047526194574132 2202 32-33 0.5421951661111137 2202 34-35 0.4468901930572642 2202 36-37 0.48968576421513177 2202 38-39 0.48719535168280004 2202 40-41 0.42974538386958727 2202 42-43 0.47451513735660455 2202 44-45 0.5424531521550051 2202 46-47 0.5032207917423577 2202 48-49 0.546784897642361 2202 50-51 0.5011339514393285 2202 52-53 0.483305950510001 2202 54-55 0.5995105682141357 2202 56-57 0.6215397824324569 2202 58-59 0.6208299279799618 2202 60-61 0.5176468389678774 2202 62-63 0.6131443379073644 2202 64-65 0.6107404361950799 2202 66-67 0.6039072381244424 2202 68-69 0.6189097979313161 2202 70-71 0.6162563700489443 2202 72-73 0.5747272963949683 2202 74-75 0.6936432985567578 2202 76-77 0.5726867920809937 2202 78-79 0.6639799915404225 2202 80-81 0.6900674775124642 2202 82-83 0.6832648540430881 2202 84-85 0.661879444198199 2202 86-87 0.6237544887731659 2202 88-89 0.778435502520729 2202 90-91 0.7654334563592755 2202 92-93 0.6387170031359481 2202 94-95 0.5724674055803085 2202 96-97 0.5841793214790272 2202 98-99 0.5456218740727614 2202 100-101 0.6758538675399919 1102 1 0.0486507418544484 1102 2 0.05809825908610833 1102 3 0.004561684315106618 1102 4 0.07939600891153731 1102 5 0.12860582159767375 1102 6 0.08170742766094463 1102 7 0.1934440471500949 1102 8 0.01003069832976422 1102 9 0.09555750014003195 1102 10-11 0.11898829845702608 1102 12-13 0.10142517687866359 1102 14-15 0.11190630026718651 1102 16-17 0.049914162806992124 1102 18-19 0.1441243173292932 1102 20-21 0.17322289053334572 1102 22-23 0.18031395247582083 1102 24-25 0.13785717297277245 1102 26-27 0.1311449387547441 1102 28-29 0.19037628490752212 1102 30-31 0.21731114422360065 1102 32-33 0.25815522483279807 1102 34-35 0.18198891318021282 1102 36-37 0.17844915918348647 1102 38-39 0.1671208214320714 1102 40-41 0.17740380363839847 1102 42-43 0.06791809360257872 1102 44-45 0.18154319154648846 1102 46-47 0.19367533811295345 1102 48-49 0.26102741304775634 1102 50-51 0.13022983821556977 1102 52-53 0.12616041803501332 1102 54-55 0.2825875240768667 1102 56-57 0.30508646505462167 1102 58-59 0.30334203041986996 1102 60-61 0.35708623783768445 1102 62-63 0.3608337805028512 1102 64-65 0.34486781167637304 1102 66-67 0.3565660787488767 1102 68-69 0.3373355817893824 1102 70-71 0.4008433593198433 1102 72-73 0.3227016258741955 1102 74-75 0.4426678482680373 1102 76-77 0.4048956104791941 1102 78-79 0.34232539819179664 1102 80-81 0.38110491856962625 1102 82-83 0.404492918900047 1102 84-85 0.3916499456021114 1102 86-87 0.3987020190324344 1102 88-89 0.4559308579940904 1102 90-91 0.4866112029203151 1102 92-93 0.4350509668879958 1102 94-95 0.4439321486630625 1102 96-97 0.4282541566839271 1102 98-99 0.5543276574866098 1102 100-101 0.42678452018093793 2305 1 0.20602259631604625 2305 2 0.1353259650397476 2305 3 0.13911993731227312 2305 4 0.21431170126091814 2305 5 0.18526059543294338 2305 6 0.3110634900262923 2305 7 0.3477643601102258 2305 8 0.29281626596702637 2305 9 0.19831234311008217 2305 10-11 0.2887400459863798 2305 12-13 0.2563857578025477 2305 14-15 0.28902722593844743 2305 16-17 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-0.9168087201698896 1213 66-67 -0.894591603130948 1213 68-69 -0.8748715598319947 1213 70-71 -0.9622995691683656 1213 72-73 -0.885433254346573 1213 74-75 -0.9665386428444123 1213 76-77 -1.0125924327818936 1213 78-79 -0.9873751193298759 1213 80-81 -1.2343405108167964 1213 82-83 -1.286360484778669 1213 84-85 -1.2243387677087512 1213 86-87 -1.1814722937419546 1213 88-89 -1.0761851441969235 1213 90-91 -1.2651857503895059 1213 92-93 -1.246523127150784 1213 94-95 -1.1149889732024754 1213 96-97 -1.2465264167793286 1213 98-99 -1.312728636799104 1213 100-101 -1.189322314740643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5950.0 3 722.0 4 697.0 5 598.0 6 901.0 7 4460.0 8 18683.0 9 50929.0 10 71713.0 11 56071.0 12 35472.0 13 26520.0 14 22352.0 15 22587.0 16 23157.0 17 23115.0 18 24087.0 19 25929.0 20 28267.0 21 30996.0 22 34207.0 23 36519.0 24 40243.0 25 45817.0 26 53422.0 27 63375.0 28 77761.0 29 96350.0 30 118922.0 31 153376.0 32 209118.0 33 299051.0 34 455241.0 35 778055.0 36 1531495.0 37 2537855.0 38 703144.0 39 599.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.35147174343911 26.263498965037613 22.376130032848497 15.008899258674777 2 32.351565969993565 24.570352407466295 29.79058187453381 13.287499748006324 3 36.44093998020645 20.25087513372085 26.197038873047234 17.111146013025465 4 30.884837273211534 19.060768991032933 31.105194637520068 18.949199098235464 5 31.117076681126036 17.839783699828377 32.72169480214313 18.321444816902456 6 27.52039326941483 18.171245724201643 36.48912740422628 17.81923360215725 7 33.311547704870826 19.087369817652082 28.295214984109318 19.305867493367778 8 29.765623405391906 27.852269200852792 23.66315532745523 18.718952066300076 9 29.39940192877062 27.234285812328963 24.100689968203906 19.265622290696516 10-11 25.049123931110177 28.583441151031636 28.68944798854739 17.677986929310798 12-13 25.58747783445916 25.827405188686605 28.516085292481137 20.069031684373098 14-15 24.388121905087274 26.56413118390237 29.02987593601783 20.01787097499253 16-17 24.28870252499952 27.550952423388146 29.733044030987614 18.42730102062472 18-19 23.620279638058868 27.397531115832198 30.157696533112283 18.82449271299665 20-21 22.91827273083566 28.772014683507674 29.517135297802888 18.792577287853778 22-23 24.370880901234152 27.635830118318687 28.841004168332706 19.152284812114452 24-25 22.860983277867057 28.617829620602315 29.21232019407234 19.30886690745829 26-27 22.17932715464736 29.210903221998148 29.222279428661913 19.38749019469258 28-29 22.387345125587895 29.2492567582907 28.655749720985536 19.70764839513587 30-31 22.73455801085205 28.957475860660242 28.83470886749531 19.4732572609924 32-33 22.646965185578168 28.58965641655477 29.36086179868352 19.40251659918354 34-35 21.729836301302022 28.48047317778098 28.98560769137759 20.804082829539407 36-37 20.4408178648925 29.16460333302836 29.489899675458496 20.904679126620643 38-39 20.606537049356728 29.4660196670846 29.4256781978311 20.501765085727573 40-41 21.496608301268402 28.222621505115704 29.925448630217684 20.355321563398217 42-43 22.298181316207323 28.270051150786895 29.90528102328922 19.526486509716566 44-45 21.127776210101974 29.023680800810027 29.95497136035312 19.89357162873488 46-47 22.090263927296935 29.275256028073628 29.010210130278587 19.624269914350855 48-49 21.401692276697037 28.942455627127593 29.21550343392801 20.440348662247363 50-51 20.749723203115362 28.644136382325758 29.123668062506454 21.482472352052433 52-53 22.909850353324742 28.349986963327726 28.102768689721668 20.637393993625867 54-55 23.576555433438855 28.107069081618054 28.315261204409687 20.001114280533404 56-57 23.776483581250694 27.642081461457312 28.216168294801086 20.365266662490907 58-59 22.78259855104892 27.690521090764612 28.55762227913157 20.969258079054896 60-61 21.198050964882498 27.869391660951038 28.214399069307163 22.718158304859305 62-63 22.077926387414266 28.010210501810302 28.56710082727395 21.344762283501485 64-65 21.330896802567416 28.784369695045626 29.39765812729177 20.487075375095188 66-67 21.44025794639054 28.32141834072866 28.681074157114434 21.55724955576637 68-69 21.163365724503507 27.482403261868672 30.662122583515362 20.692108430112466 70-71 20.95942914217189 29.624603539324262 29.308807853719504 20.10715946478435 72-73 21.057298052238156 31.119226803526683 27.983214285295986 19.84026085893917 74-75 21.87132003730699 30.692676583878065 27.558501687390386 19.87750169142456 76-77 21.956574247303266 29.845223333781014 27.770472653675693 20.42772976524003 78-79 21.68847160217782 29.774149944582362 28.33107244467688 20.20630600856294 80-81 21.121340419641296 30.492583964640136 28.10549124652836 20.280584369190205 82-83 20.477064979796232 30.362613309132776 28.407648146367748 20.75267356470325 84-85 20.67833722243376 29.951879297944146 28.57869814397157 20.79108533565052 86-87 20.538454233186705 30.126771653697066 29.05423213222281 20.280541980893414 88-89 20.58850636778462 30.496971375853615 28.894672925384974 20.01984933097679 90-91 21.061020768345085 29.509110187055533 28.738133578074592 20.69173546652479 92-93 20.753512224225418 29.965425907407273 28.196773041316497 21.084288827050813 94-95 20.486993686321195 30.497712989708326 27.790669195816697 21.22462412815378 96-97 20.8045995407798 30.8172120914974 27.49821019668709 20.879978171035713 98-99 21.06208083749241 29.922051220248786 27.843358528011148 21.172509414247656 100-101 21.376846778921806 30.04710086820869 27.348912213760336 21.22714013910917 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6152.0 1 3913.0 2 1464.0 3 1420.0 4 1420.5 5 1302.0 6 1510.0 7 1951.0 8 2400.5 9 2644.5 10 2908.5 11 3234.5 12 3326.0 13 3224.5 14 3036.5 15 2812.5 16 2627.5 17 2530.0 18 2659.5 19 2939.0 20 3485.0 21 4459.5 22 5706.0 23 7458.0 24 10086.5 25 13487.5 26 17797.0 27 23160.0 28 29453.5 29 36994.5 30 45755.5 31 56108.5 32 71018.5 33 93227.0 34 124883.0 35 176208.5 36 246423.0 37 328053.5 38 408903.5 39 449746.5 40 467595.5 41 455056.5 42 429957.5 43 445784.5 44 447442.0 45 438463.5 46 453975.0 47 424979.0 48 341571.5 49 273807.0 50 230521.5 51 205175.5 52 186577.5 53 159670.5 54 126548.5 55 94321.5 56 72113.0 57 59776.0 58 50229.5 59 38740.5 60 27893.5 61 20578.5 62 14963.5 63 10588.5 64 7724.0 65 5544.0 66 4015.5 67 3052.5 68 2231.5 69 1445.0 70 961.5 71 731.5 72 580.5 73 459.0 74 347.0 75 273.0 76 219.0 77 166.5 78 145.5 79 125.0 80 105.0 81 84.0 82 68.5 83 59.5 84 52.0 85 41.5 86 33.0 87 29.0 88 26.5 89 21.0 90 17.0 91 12.0 92 9.5 93 9.5 94 8.0 95 6.5 96 2.5 97 1.0 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25995892968070083 2 0.24742609911367197 3 0.21225373506893577 4 0.21553614307458618 5 0.22966476883803794 6 0.23306394234586564 7 0.20941244118262178 8 0.20523483099361214 9 0.18627989780683252 10-11 0.17438279052943553 12-13 0.2028605991160073 14-15 0.1997273914742501 16-17 0.20054474998949112 18-19 0.1926306437308083 20-21 0.1934090804119902 22-23 0.16026713871067014 24-25 0.46677009495370636 26-27 0.3250556971445386 28-29 0.2766888313537688 30-31 0.2808988764044944 32-33 0.32237657756680416 34-35 0.31857521177369913 36-37 0.31128385485996185 38-39 0.33517537399990344 40-41 0.3400081683955746 42-43 0.3760951955406995 44-45 0.3442441613356728 46-47 0.3700169024551374 48-49 0.3461902530386276 50-51 0.3683108287288803 52-53 0.35676401795801527 54-55 0.33302818615431 56-57 0.3699779806210783 58-59 0.35691970529425165 60-61 0.3227593089350519 62-63 0.2999446272040786 64-65 0.30626942523868167 66-67 0.3152538819339896 68-69 0.3217278803324859 70-71 0.2989261725461989 72-73 0.3062240164322794 74-75 0.30365517538437903 76-77 0.3068597397219113 78-79 0.3091755888484275 80-81 0.3262557870280273 82-83 0.34240186119020893 84-85 0.33772475413077424 86-87 0.34079309204909963 88-89 0.33583055820656493 90-91 0.31130980274933456 92-93 0.29594865224067807 94-95 0.3047320127933474 96-97 0.2926143484562822 98-99 0.31210770034754604 100-101 0.333845544669551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7707756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.811175096135194 #Duplication Level Percentage of deduplicated Percentage of total 1 89.38150583123459 40.94671813990917 2 5.370396714280984 4.920483684272706 3 1.726636988307059 2.372978081963946 4 0.78107604425421 1.4312804570690503 5 0.5089064104030697 1.1656800337260333 6 0.2788505110031426 0.7664681751127045 7 0.21835897217175487 0.7002296775580663 8 0.1942108086547668 0.7117620288676425 9 0.12145299855107036 0.5007514124316365 >10 1.079500747640008 10.159183770293177 >50 0.16461787977949238 5.265784611525554 >100 0.14152327412082227 13.24522224306833 >500 0.020031336692715087 6.388717187982324 >1k 0.012504977970456022 9.825788559719898 >5k 2.8447568397741693E-4 0.7963591399191178 >10k+ 1.4202925200172228E-4 0.802592796580849 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 15505 0.20116101236209347 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 14511 0.18826491134384638 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10771 0.13974235821684028 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10728 0.13918447859532657 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 10161 0.13182825195815748 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8991 0.11664873667511012 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8624 0.11188729897521406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0023093621541730174 0.0 0.0 2 0.002529919213841227 0.0 0.0 3 0.0027893981075685324 0.0 0.0 4 0.0030748248906685683 0.0 0.0 5 0.0035808087334368134 0.0 0.0 6 0.004203558078382346 0.0 0.0 7 0.004800359533955149 0.0 0.0 8 0.005578796215137065 0.0 0.0 9 0.006370206841005346 0.0 0.0 10-11 0.01071647831093771 0.0 0.0 12-13 0.014394591629522262 0.0 0.0 14-15 0.01865004548665007 0.0 0.0 16-17 0.023833136388852996 0.0 6.486972343182633E-6 18-19 0.028639982895151326 0.0 1.2973944686365266E-5 20-21 0.032966793448054144 0.0 1.2973944686365266E-5 22-23 0.03794230123527522 0.0 1.2973944686365266E-5 24-25 0.043527584422755464 0.0 1.2973944686365266E-5 26-27 0.04984589548501535 0.0 1.2973944686365266E-5 28-29 0.05735132248607766 0.0 1.94609170295479E-5 30-31 0.1051473347106473 0.0 3.89218340590958E-5 32-33 0.11605842219188048 0.0 5.1895778745461064E-5 34-35 0.12421254642726107 0.0 5.1895778745461064E-5 36-37 0.13367055210362133 0.0 5.1895778745461064E-5 38-39 0.1441405254655181 0.0 5.1895778745461064E-5 40-41 0.1553046048681354 0.0 5.1895778745461064E-5 42-43 0.1676752611265847 0.0 5.1895778745461064E-5 44-45 0.18093463259605 0.0 5.1895778745461064E-5 46-47 0.19486864918920632 0.0 5.1895778745461064E-5 48-49 0.2106774007895424 0.0 5.1895778745461064E-5 50-51 0.2280819475863014 0.0 5.1895778745461064E-5 52-53 0.24629087895361504 0.0 5.83827510886437E-5 54-55 0.2667183548622971 0.0 7.78436681181916E-5 56-57 0.2881577984565158 1.2973944686365266E-5 9.081761280455686E-5 58-59 0.3116341513664937 1.2973944686365266E-5 9.081761280455686E-5 60-61 0.337076056896456 1.2973944686365266E-5 9.081761280455686E-5 62-63 0.36433431468250943 1.2973944686365266E-5 1.0379155749092213E-4 64-65 0.39383706489930403 1.2973944686365266E-5 1.0379155749092213E-4 66-67 0.4267195277068968 1.2973944686365266E-5 1.0379155749092213E-4 68-69 0.4624757192625195 1.2973944686365266E-5 1.0379155749092213E-4 70-71 0.5018711023026676 1.2973944686365266E-5 1.167655021772874E-4 72-73 0.5453857127807367 1.2973944686365266E-5 1.2973944686365266E-4 74-75 0.5941223359950678 1.2973944686365266E-5 1.2973944686365266E-4 76-77 0.6474971444347746 1.2973944686365266E-5 1.556873362363832E-4 78-79 0.7083773798755435 1.2973944686365266E-5 1.6217430857956583E-4 80-81 0.7749856118953429 1.2973944686365266E-5 1.6866128092274846E-4 82-83 0.8500917776847114 1.2973944686365266E-5 1.6866128092274846E-4 84-85 0.9325801180006217 1.2973944686365266E-5 1.6866128092274846E-4 86-87 1.0223663022026126 1.2973944686365266E-5 1.6866128092274846E-4 88-89 1.1219283537257796 1.2973944686365266E-5 1.8163522560911373E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4465 0.0 77.26389 2 GAGCGGC 2910 0.0 74.3342 9 CGGGAGA 3855 0.0 70.15849 4 AGAGCGG 3240 0.0 68.816444 8 CGGAAAG 1805 0.0 63.0876 5 AAGCGGC 1235 0.0 62.22499 9 ATTCGGA 875 0.0 60.197514 2 TCGGGAA 1605 0.0 57.94582 3 GTTCGGA 655 0.0 57.233383 2 GGATCGG 1810 0.0 55.06062 1 TCGGGAG 5160 0.0 54.16349 3 GATCGGG 7015 0.0 52.361687 1 TCGGAGA 1230 0.0 51.306957 4 TCGGAAA 1895 0.0 50.579193 4 GAGAGCG 4855 0.0 50.12717 7 AAGCGTC 540 0.0 49.19388 9 TTGTGTT 62375 0.0 45.117443 1 GGTTCGG 1045 0.0 44.50514 1 GATCCGG 1200 0.0 43.10678 1 CGGAGAG 2040 0.0 42.562866 5 >>END_MODULE