FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6120387
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC135650.221636311560037No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG127390.20814043294974646No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA98470.1608885189776398No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT96220.1572122808574033Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC29750.078.28459
AGAGCGG31600.075.503888
CGGGAGA39900.074.794434
GAGAGCG38250.070.8143847
GATCGGG55250.068.025751
CGGAAAG20400.066.979695
TCGGGAG46150.065.4892353
GTTCGGA9500.065.4307562
AAGCGGC12400.065.0292059
ATCGGGA57700.063.56792
GATCCGG9750.059.3793831
GGTTCGG12500.058.464361
AGAGCGT77300.058.0497068
GAGCGTC76300.057.9392179
TCGGGAA15550.057.057593
TCGGAAA22750.056.099944
GGATCGG23200.055.4321561
AAGGGGC34450.054.110889
AAGAGCG79300.053.2394567
AAGCGTC5150.052.498749
Produced by FastQC (version 0.11.2)