##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_35011-1.342000000358a4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6120387 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53547120468036 33.0 33.0 33.0 27.0 33.0 2 31.319316736016855 33.0 33.0 33.0 27.0 33.0 3 31.222747679190874 33.0 33.0 33.0 27.0 33.0 4 34.77861105841836 37.0 37.0 37.0 33.0 37.0 5 34.77041141352663 37.0 37.0 37.0 33.0 37.0 6 35.02226967020223 37.0 37.0 37.0 33.0 37.0 7 34.784112344529845 37.0 37.0 37.0 33.0 37.0 8 34.928131015244624 37.0 37.0 37.0 33.0 37.0 9 34.582136227660115 37.0 37.0 37.0 33.0 37.0 10-11 34.55588510987948 37.0 37.0 37.0 33.0 37.0 12-13 34.566602242635966 37.0 37.0 37.0 33.0 37.0 14-15 36.27113824665009 40.0 37.0 40.0 33.0 40.0 16-17 36.225223503023585 40.0 37.0 40.0 33.0 40.0 18-19 36.25954004215747 40.0 37.0 40.0 33.0 40.0 20-21 36.24325773844039 40.0 37.0 40.0 30.0 40.0 22-23 36.587083300451425 40.0 37.0 40.0 33.0 40.0 24-25 36.2067620887372 40.0 37.0 40.0 27.0 40.0 26-27 36.09962131479594 40.0 37.0 40.0 27.0 40.0 28-29 35.89868933124654 40.0 37.0 40.0 27.0 40.0 30-31 35.909828822915934 40.0 37.0 40.0 27.0 40.0 32-33 35.74229766843175 40.0 37.0 40.0 27.0 40.0 34-35 35.82190448087678 40.0 37.0 40.0 27.0 40.0 36-37 35.6392051679085 40.0 37.0 40.0 27.0 40.0 38-39 35.58098523835176 40.0 37.0 40.0 27.0 40.0 40-41 35.48678171821488 40.0 37.0 40.0 27.0 40.0 42-43 35.36882643205405 38.5 37.0 40.0 27.0 40.0 44-45 35.26207305191649 37.0 37.0 40.0 24.5 40.0 46-47 35.19298436520436 37.0 37.0 40.0 24.5 40.0 48-49 35.089684361462766 37.0 37.0 40.0 22.0 40.0 50-51 34.05823479789758 37.0 35.0 38.5 22.0 38.5 52-53 34.47070846990558 37.0 35.0 38.5 24.5 40.0 54-55 35.19081750876211 37.0 37.0 40.0 27.0 40.0 56-57 35.356282780811085 37.0 37.0 40.0 27.0 40.0 58-59 35.043973934981565 37.0 37.0 40.0 27.0 40.0 60-61 34.82562409860684 37.0 37.0 40.0 22.0 40.0 62-63 34.562990134447375 37.0 37.0 40.0 22.0 40.0 64-65 34.34279368281777 37.0 33.0 40.0 22.0 40.0 66-67 34.147081957399095 37.0 33.0 40.0 22.0 40.0 68-69 34.04740639766733 37.0 33.0 37.0 22.0 40.0 70-71 33.709912379723704 37.0 33.0 37.0 22.0 40.0 72-73 33.683017593495315 37.0 33.0 37.0 24.5 40.0 74-75 33.5806047885534 37.0 33.0 37.0 27.0 38.5 76-77 33.314698155525136 37.0 33.0 37.0 22.0 37.0 78-79 32.96251585071336 37.0 33.0 37.0 22.0 37.0 80-81 32.60899376134222 37.0 33.0 37.0 22.0 37.0 82-83 32.30032684207714 37.0 33.0 37.0 22.0 37.0 84-85 32.13293562972407 37.0 33.0 37.0 22.0 37.0 86-87 31.961672113217677 37.0 33.0 37.0 15.0 37.0 88-89 31.731197308274787 33.0 33.0 37.0 15.0 37.0 90-91 31.666485387280247 33.0 33.0 37.0 15.0 37.0 92-93 31.523084651346394 33.0 33.0 37.0 15.0 37.0 94-95 31.391669105237952 33.0 33.0 37.0 15.0 37.0 96-97 31.247259772952265 33.0 33.0 37.0 15.0 37.0 98-99 31.113935115540897 33.0 33.0 37.0 15.0 37.0 100-101 29.91548238697978 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2511607170479522 2306 2 0.23392520034586894 2306 3 0.1949788594578017 2306 4 0.31892728513111734 2306 5 0.2774592122032473 2306 6 0.2515368000008138 2306 7 0.5267132836942139 2306 8 0.1776512464836415 2306 9 0.42024338394885774 2306 10-11 0.3547630109239108 2306 12-13 0.33031077127157715 2306 14-15 0.5211958166917583 2306 16-17 0.4940301421751556 2306 18-19 0.5327962234131576 2306 20-21 0.55260725395582 2306 22-23 0.3989328385948312 2306 24-25 0.4554987048968542 2306 26-27 0.4610365542107999 2306 28-29 0.4633620610794793 2306 30-31 0.46980378639588594 2306 32-33 0.4933818201048936 2306 34-35 0.30789882452075545 2306 36-37 0.4728816050920486 2306 38-39 0.34213640676905044 2306 40-41 0.3370722735559255 2306 42-43 0.36584997941454134 2306 44-45 0.41538361079968666 2306 46-47 0.3964599872987762 2306 48-49 0.3733093797413076 2306 50-51 0.4261349459813104 2306 52-53 0.38561364890382777 2306 54-55 0.45097110253854567 2306 56-57 0.42743305028788114 2306 58-59 0.5355031671971275 2306 60-61 0.46757846043646367 2306 62-63 0.44781589793567633 2306 64-65 0.4017789584087197 2306 66-67 0.40715316046478023 2306 68-69 0.32362597239900737 2306 70-71 0.4490776584798226 2306 72-73 0.22130561806805815 2306 74-75 0.38103931518918444 2306 76-77 0.3873247650604199 2306 78-79 0.3242426689719551 2306 80-81 0.41276687941836343 2306 82-83 0.3696312265746471 2306 84-85 0.3194595812108716 2306 86-87 0.3202365934761602 2306 88-89 0.4295440464859617 2306 90-91 0.36536950698491566 2306 92-93 0.251138865255367 2306 94-95 0.14203703757200614 2306 96-97 0.25356866316927906 2306 98-99 0.3054632805392927 2306 100-101 0.18824645691139352 2307 1 0.20006643570895832 2307 2 0.15978848428684245 2307 3 0.11430251908492295 2307 4 0.2303393332393 2307 5 0.2244840429426347 2307 6 0.1301196620078997 2307 7 0.3959402793897553 2307 8 0.12384989239878763 2307 9 0.2661981103017368 2307 10-11 0.1966301868715803 2307 12-13 0.1031958583480943 2307 14-15 0.3485026533722717 2307 16-17 0.3803296424011293 2307 18-19 0.31062082423017046 2307 20-21 0.16333806925882755 2307 22-23 0.1735558141590019 2307 24-25 0.3958030229619922 2307 26-27 0.2697345748599318 2307 28-29 0.25952448544423135 2307 30-31 0.30140560822358964 2307 32-33 0.367617832794501 2307 34-35 0.30023273412264473 2307 36-37 0.3519505291518428 2307 38-39 0.10026986705697283 2307 40-41 0.2891343131078372 2307 42-43 0.2558211513605002 2307 44-45 0.22342288628091467 2307 46-47 0.16345261135375466 2307 48-49 0.10456653739171173 2307 50-51 0.18540373577345548 2307 52-53 0.16900215190729995 2307 54-55 0.11852931809517031 2307 56-57 0.09426209597837243 2307 58-59 0.1806078827998263 2307 60-61 0.23180181423170865 2307 62-63 0.11417204506844314 2307 64-65 0.13192187008605316 2307 66-67 0.2136605561550553 2307 68-69 0.1263167004719108 2307 70-71 0.1168358494645858 2307 72-73 -0.11576631604426524 2307 74-75 -0.050570492492148844 2307 76-77 0.0020596742103649035 2307 78-79 0.05528646830763506 2307 80-81 0.11705023681163595 2307 82-83 0.1087930190916353 2307 84-85 0.28781962912599823 2307 86-87 0.2563050085474785 2307 88-89 0.33164897709240293 2307 90-91 0.25471762877357307 2307 92-93 0.25064949038974405 2307 94-95 0.07731454598567566 2307 96-97 0.14098675332166621 2307 98-99 0.10090526682293444 2307 100-101 -0.03663658543556991 2201 1 0.18818409565868777 2201 2 0.11777113105504355 2201 3 0.1217328017869157 2201 4 0.27475579434004516 2201 5 0.14596219916654718 2201 6 0.1546870057880838 2201 7 0.4057345855141392 2201 8 0.10583123782333814 2201 9 0.2906717325638368 2201 10-11 0.21919311261940067 2201 12-13 0.24258683310444695 2201 14-15 0.4625104589077864 2201 16-17 0.3487011768289747 2201 18-19 0.31732219801240547 2201 20-21 0.3113327812462856 2201 22-23 0.19592695715688535 2201 24-25 0.3301810904316298 2201 26-27 0.35992645907359844 2201 28-29 0.3149015558391568 2201 30-31 0.272510551100531 2201 32-33 0.4071798248722658 2201 34-35 0.2965242958976191 2201 36-37 0.35667845375116514 2201 38-39 0.38278185149236066 2201 40-41 0.36343471559285945 2201 42-43 0.30180746826335536 2201 44-45 0.3295023199943117 2201 46-47 0.3489770661705833 2201 48-49 0.3229169335423805 2201 50-51 0.33666694495553173 2201 52-53 0.32091366950357525 2201 54-55 0.4696549889120263 2201 56-57 0.5235634929301938 2201 58-59 0.6126521622237604 2201 60-61 0.5416944790519054 2201 62-63 0.5070371644417477 2201 64-65 0.4496256122110509 2201 66-67 0.4206964220353271 2201 68-69 0.4401769231403847 2201 70-71 0.4271576569663722 2201 72-73 0.2662330565105435 2201 74-75 0.33226361280087957 2201 76-77 0.31076841214051143 2201 78-79 0.3753097568824231 2201 80-81 0.5302792287564415 2201 82-83 0.43903097517364387 2201 84-85 0.35425734634853967 2201 86-87 0.4868011022142369 2201 88-89 0.6057596627757356 2201 90-91 0.47356377100640756 2201 92-93 0.4230136103860467 2201 94-95 0.3514605809807172 2201 96-97 0.3706080381141028 2201 98-99 0.44125023291184107 2201 100-101 0.3114569905289173 1101 1 0.08781037490663124 1101 2 0.04166877528558999 1101 3 0.036083551042707995 1101 4 0.12748337963774503 1101 5 0.11775097763619868 1101 6 0.17694222628491474 1101 7 0.4003160744940075 1101 8 0.11137654135944786 1101 9 0.2316267082695589 1101 10-11 0.167184733136871 1101 12-13 0.17672574823853182 1101 14-15 0.34284146968767715 1101 16-17 0.3229060361540448 1101 18-19 0.2812279028940168 1101 20-21 0.24985404295038194 1101 22-23 0.28232546558150773 1101 24-25 0.4046571754865269 1101 26-27 0.28159424659667565 1101 28-29 0.36263746552996423 1101 30-31 0.35765482176471863 1101 32-33 0.3927303488768885 1101 34-35 0.23911022435242302 1101 36-37 0.3827297659045641 1101 38-39 0.28469866510506137 1101 40-41 0.299688871984074 1101 42-43 0.30631251056912134 1101 44-45 0.355043441416349 1101 46-47 0.24534102457856477 1101 48-49 0.26538503708768957 1101 50-51 0.2676147326054803 1101 52-53 0.346984125136899 1101 54-55 0.42362150851371894 1101 56-57 0.5025731391673673 1101 58-59 0.4557641134831769 1101 60-61 0.5318270912612988 1101 62-63 0.5159884404339721 1101 64-65 0.4396398331358 1101 66-67 0.44565568739087524 1101 68-69 0.34629359725111186 1101 70-71 0.44328632199410123 1101 72-73 0.2896892188665845 1101 74-75 0.5036454847107521 1101 76-77 0.4521153534296545 1101 78-79 0.38282049544958596 1101 80-81 0.541601200075732 1101 82-83 0.5996027656210359 1101 84-85 0.5775057774044186 1101 86-87 0.5355990030618116 1101 88-89 0.5778363486111822 1101 90-91 0.5354675549700865 1101 92-93 0.5786573140520765 1101 94-95 0.4837284720447279 1101 96-97 0.429319788393407 1101 98-99 0.44006511179836494 1101 100-101 0.36905751277211607 2304 1 0.22789843657695386 2304 2 0.20512825657434774 2304 3 0.1872017317000676 2304 4 0.34425479707687145 2304 5 0.3605309888127337 2304 6 0.278207222823589 2304 7 0.5485813366049754 2304 8 0.2862243521441812 2304 9 0.3522447345945352 2304 10-11 0.3987795524071842 2304 12-13 0.37446875755309605 2304 14-15 0.5264590551788757 2304 16-17 0.556732159898452 2304 18-19 0.47789035634966126 2304 20-21 0.4542159946750459 2304 22-23 0.4130003630262067 2304 24-25 0.5481668386893048 2304 26-27 0.5291022450621412 2304 28-29 0.5083886421005488 2304 30-31 0.4752065654278965 2304 32-33 0.5508325941740679 2304 34-35 0.487886594110023 2304 36-37 0.5834708046381678 2304 38-39 0.4737618931389136 2304 40-41 0.39970314680332564 2304 42-43 0.49319780601362595 2304 44-45 0.4423997635796937 2304 46-47 0.41219862165465315 2304 48-49 0.4841121893984237 2304 50-51 0.41976133147286276 2304 52-53 0.476453562054175 2304 54-55 0.536902478744409 2304 56-57 0.5496843096471835 2304 58-59 0.584455435221777 2304 60-61 0.5733534326579175 2304 62-63 0.4968259413997629 2304 64-65 0.5999982263116479 2304 66-67 0.5687736408077839 2304 68-69 0.49079733164327166 2304 70-71 0.5143462170974544 2304 72-73 0.44690131563335456 2304 74-75 0.5154275001805289 2304 76-77 0.6652901194282279 2304 78-79 0.5497291369942374 2304 80-81 0.4022797046628739 2304 82-83 0.5559413725150506 2304 84-85 0.5990686006970734 2304 86-87 0.4337155662001848 2304 88-89 0.4030985611369253 2304 90-91 0.5679575443475393 2304 92-93 0.5849900900632683 2304 94-95 0.5499616762546538 2304 96-97 0.49426469721811017 2304 98-99 0.4501141543363758 2304 100-101 0.5261351307265372 2202 1 0.1807749544708983 2202 2 0.02736065929932252 2202 3 0.05035735648274908 2202 4 0.20698592960852835 2202 5 0.1938013508758658 2202 6 0.19634200672489754 2202 7 0.24755868804130898 2202 8 0.17080388426145277 2202 9 0.14303320299836741 2202 10-11 0.16720461882884052 2202 12-13 0.20219377293883412 2202 14-15 0.306334550497219 2202 16-17 0.20682990355913233 2202 18-19 0.2572679795353352 2202 20-21 0.2203071331182187 2202 22-23 0.23321668459571754 2202 24-25 0.26993956330272084 2202 26-27 0.3055016566168405 2202 28-29 0.21357995116314754 2202 30-31 0.1473597311470911 2202 32-33 0.22498605400978278 2202 34-35 0.13083731971018864 2202 36-37 0.26422649702652024 2202 38-39 0.15769860754301845 2202 40-41 0.12847299552237246 2202 42-43 0.08326244863155097 2202 44-45 0.20213850931138921 2202 46-47 0.10709644966322429 2202 48-49 0.1343308398878449 2202 50-51 0.13564807751075136 2202 52-53 0.1329091044921853 2202 54-55 0.17488861175432646 2202 56-57 0.256188728890443 2202 58-59 0.29006440432945624 2202 60-61 0.19335786297863677 2202 62-63 0.27499839330866394 2202 64-65 0.2717001845279938 2202 66-67 0.2673959828640946 2202 68-69 0.2772946938147882 2202 70-71 0.2682549685429976 2202 72-73 0.22538625804847356 2202 74-75 0.35144129141935565 2202 76-77 0.2710548230318821 2202 78-79 0.34465772319591537 2202 80-81 0.3001716242062855 2202 82-83 0.2747673600772558 2202 84-85 0.20160376035644134 2202 86-87 0.19680748232085676 2202 88-89 0.4247301210993548 2202 90-91 0.32727040485419323 2202 92-93 0.233705058259023 2202 94-95 0.21348724177225975 2202 96-97 0.24253963155265623 2202 98-99 0.2803670693335185 2202 100-101 0.34839559192829483 1102 1 0.0460265656726655 1102 2 0.04979671522447404 1102 3 0.047726054474498625 1102 4 0.080949124599762 1102 5 0.13499232789239102 1102 6 0.07732050670650636 1102 7 0.1511764905687727 1102 8 -0.03194273452878349 1102 9 0.027510448379040042 1102 10-11 0.08002906218287364 1102 12-13 0.07794070110008278 1102 14-15 0.19720167056995308 1102 16-17 0.08224527219879718 1102 18-19 0.10913980103332221 1102 20-21 0.19172898460237775 1102 22-23 0.2243165302905794 1102 24-25 0.17245900359474575 1102 26-27 0.20945919671145674 1102 28-29 0.09223988013460627 1102 30-31 0.15043908227940506 1102 32-33 0.14782213681471745 1102 34-35 0.12141434105045334 1102 36-37 0.16286190454042782 1102 38-39 0.16754148763512688 1102 40-41 0.19439843964096326 1102 42-43 0.12239994376592733 1102 44-45 0.20587574934146602 1102 46-47 0.15263320205355768 1102 48-49 0.16305758792922376 1102 50-51 0.1283809964897742 1102 52-53 0.17258952041871112 1102 54-55 0.3269255473582433 1102 56-57 0.36627390735568355 1102 58-59 0.3242682240472945 1102 60-61 0.27184259792754517 1102 62-63 0.31266992622941103 1102 64-65 0.38199697784659037 1102 66-67 0.4457836906910515 1102 68-69 0.3664427760314126 1102 70-71 0.3092988675308206 1102 72-73 0.3740612561783081 1102 74-75 0.4690159747261262 1102 76-77 0.38854582401197746 1102 78-79 0.2882460086845171 1102 80-81 0.3821848688363474 1102 82-83 0.3581137212273475 1102 84-85 0.410820607891047 1102 86-87 0.34920203307989794 1102 88-89 0.4819647752187457 1102 90-91 0.42137967622203476 1102 92-93 0.39344289167286917 1102 94-95 0.35198675425931114 1102 96-97 0.3667974897668991 1102 98-99 0.3537744742050961 1102 100-101 0.36233516568095325 2305 1 0.22031114210777858 2305 2 0.0764508655345395 2305 3 0.07843664217433499 2305 4 0.1010378769465774 2305 5 0.05932526297801388 2305 6 0.23775175208399446 2305 7 0.1513244111467955 2305 8 0.20998706008918333 2305 9 0.0872020908663842 2305 10-11 0.18239863605781892 2305 12-13 0.11693484116949548 2305 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-0.6538611694360483 1213 62-63 -0.8115648167365279 1213 64-65 -0.5921733818173394 1213 66-67 -0.5630395706342597 1213 68-69 -0.411934779226101 1213 70-71 -0.5308635086036588 1213 72-73 -0.38650606922358577 1213 74-75 -0.6577347808734118 1213 76-77 -0.6904176916346145 1213 78-79 -0.6697898231549857 1213 80-81 -0.9529673289491498 1213 82-83 -1.1044138948687632 1213 84-85 -1.0213082513091791 1213 86-87 -0.9719541752789347 1213 88-89 -0.8236116868247656 1213 90-91 -1.0190596927926698 1213 92-93 -0.9964381511340719 1213 94-95 -0.9205189715141167 1213 96-97 -0.9903051458973984 1213 98-99 -1.0599928459882548 1213 100-101 -0.8966226495114782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4508.0 3 672.0 4 639.0 5 488.0 6 1110.0 7 6506.0 8 25162.0 9 66146.0 10 87879.0 11 64645.0 12 41810.0 13 29127.0 14 21483.0 15 17725.0 16 15844.0 17 15548.0 18 16194.0 19 17617.0 20 19401.0 21 21549.0 22 24224.0 23 25970.0 24 28751.0 25 32805.0 26 38086.0 27 45136.0 28 55133.0 29 68074.0 30 84843.0 31 109256.0 32 149006.0 33 212658.0 34 321699.0 35 544583.0 36 1074934.0 37 2047127.0 38 783010.0 39 1039.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.354269288141275 24.883570527838724 23.373681353297883 15.388478830722113 2 30.25907560649545 24.850237219265424 32.00142433109998 12.889262843139148 3 37.0196256930965 20.711643202558143 26.546547677425746 15.722183426919612 4 28.301322366066266 19.692942283327106 34.33177467414046 17.673960676466166 5 32.99281043728714 18.404743411559927 31.64167440842867 16.96077174272426 6 27.557352742250917 18.836015193404094 37.195934582025814 16.41069748231918 7 33.311028608551915 19.522468230911695 29.314679008432464 17.851824152103923 8 27.77611332347103 28.58181289525333 26.44750223800889 17.194571543266754 9 30.84857091786927 25.88842532846988 25.41707342783942 17.84593032582143 10-11 24.676045448400497 29.905619707618474 29.196507277786672 16.221827566194357 12-13 25.911677898584657 26.299018628765193 29.067744804187427 18.721558668462727 14-15 23.817398991552615 26.598831117804988 30.324741339794382 19.25902855084801 16-17 23.980969380275397 27.343267631370843 31.180463713171108 17.495299275182653 18-19 23.63429212186443 27.5515922628487 31.396931085497037 17.417184529789832 20-21 23.09638498105636 28.825640019522723 30.59916151813329 17.478813481287624 22-23 24.632173951891936 27.68927001992079 29.918719636257773 17.7598363919295 24-25 22.679391198929025 29.37703198397149 29.88244554631795 18.061131270781537 26-27 21.7108234833007 30.558284219432608 29.56624448669878 18.164647810567917 28-29 22.23790256681516 29.679489915395774 29.39395053998612 18.68865697780295 30-31 23.24008305237551 28.312454866178605 29.72305088453074 18.72441119691515 32-33 21.756779262060654 29.35117390500444 30.683389545793705 18.208657287141204 34-35 23.835234795399447 28.19213406338156 29.880408147978105 18.09222299324089 36-37 22.420009607648357 27.957617519551697 30.679022432433005 18.94335044036694 38-39 21.25930487683623 28.917794451660956 31.056396613312952 18.766504058189863 40-41 21.54679601937766 29.81009012462254 29.543248514611488 19.099865341388313 42-43 22.630125425020843 28.445219134613936 30.3302107736019 18.594444666763323 44-45 21.511767980570447 29.090839486087784 30.79122863293143 18.606163900410348 46-47 22.214611907715977 29.419568974855103 29.605572969627904 18.76024614780102 48-49 20.79395033926664 29.426833257230268 30.21606985195563 19.56314655154746 50-51 20.75407617608241 28.261022861745865 30.693076207568804 20.291824754602917 52-53 23.063980475933153 27.961124039101588 28.993988627904994 19.98090685706026 54-55 23.84974140703067 28.10322013963701 28.699946664201104 19.34709178913122 56-57 23.941577732812146 28.319352888490634 28.66147124751968 19.07759813117754 58-59 23.164783547233434 28.316570672135533 28.942131822213717 19.57651395841732 60-61 21.336467856034663 28.685349444806246 28.575508924193976 21.402673774965113 62-63 22.3380227195053 28.483131295411006 28.80684700473041 20.371998980353283 64-65 21.081446775788564 29.02157406536366 29.921195448202937 19.975783710644844 66-67 20.57579004890611 28.365642924086533 29.603547110854834 21.455019916152523 68-69 20.206686592294705 28.096733711585877 31.908559135808662 19.78802056031076 70-71 19.866016065322555 30.47114791271502 30.20035808693314 19.462477935029288 72-73 19.910672106154415 32.100598564902846 28.32791474147888 19.660814587463857 74-75 20.111641844075077 31.712031169645062 28.40121511008002 19.775111876199844 76-77 19.957103033845076 31.668307493923315 28.510142508592423 19.86444696363919 78-79 19.784392534997984 31.782400880051874 28.598498211534178 19.834708373415964 80-81 19.600320370544008 31.903342646328888 28.45046649124751 20.045870491879597 82-83 19.385514396585666 31.448292279884782 28.57628740657502 20.58990591695453 84-85 19.523065339876297 31.13974374895743 28.879773660350356 20.457417250815922 86-87 19.91643801119581 30.90290984853652 29.15339218916409 20.02725995110358 88-89 20.195396030420767 31.006688919620746 28.669989390487043 20.127925659471437 90-91 20.270717998874844 30.740338129116136 28.630409494265013 20.358534377744007 92-93 19.913018534136402 31.283642350316914 28.654394478047706 20.14894463749898 94-95 19.579438494168333 31.39851528351359 28.766345049658533 20.255701172659542 96-97 19.844826781001665 31.46768515051532 28.650667304652906 20.036820763830114 98-99 20.384292630799138 30.891689366598158 28.20341924888631 20.520598753716396 100-101 20.567332518617786 30.819485860335437 27.60452890913665 21.008652711910127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3267.0 1 2096.5 2 769.5 3 572.0 4 582.5 5 655.0 6 813.0 7 1111.0 8 1448.5 9 1838.5 10 2234.5 11 2492.5 12 2552.5 13 2477.5 14 2338.5 15 2245.5 16 2168.0 17 2128.0 18 2246.5 19 2491.5 20 2944.5 21 3639.0 22 4615.0 23 6123.0 24 8396.0 25 11672.0 26 16828.0 27 23638.5 28 31764.5 29 41137.0 30 52087.0 31 64335.0 32 82118.5 33 110596.0 34 147068.0 35 192968.0 36 255596.5 37 319233.0 38 374735.5 39 406380.5 40 412475.5 41 399398.0 42 371789.5 43 350157.5 44 322466.5 45 295475.5 46 281730.0 47 258218.0 48 225266.5 49 198081.5 50 168046.0 51 139996.5 52 115783.0 53 91814.5 54 74283.5 55 59821.5 56 44544.0 57 31733.0 58 23083.5 59 17132.0 60 12699.5 61 9315.5 62 6681.0 63 4784.5 64 3438.0 65 2583.5 66 1925.0 67 1430.0 68 1097.0 69 820.5 70 638.0 71 502.5 72 376.0 73 315.0 74 261.5 75 213.0 76 175.5 77 146.0 78 136.0 79 121.0 80 96.5 81 81.5 82 70.0 83 63.0 84 57.0 85 46.5 86 34.5 87 31.0 88 29.5 89 26.5 90 25.0 91 20.0 92 19.5 93 16.0 94 12.5 95 11.0 96 6.5 97 4.0 98 3.0 99 3.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25869279181202104 2 0.24614456569494705 3 0.20965994470611093 4 0.21242120800531078 5 0.22704446630580716 6 0.22960966357192772 7 0.205264797797917 8 0.20062456834837403 9 0.18219436123892166 10-11 0.17033236623762518 12-13 0.19931746146117885 14-15 0.19585362821011154 16-17 0.19640097921912453 18-19 0.1887053874207628 20-21 0.18966937875006923 22-23 0.15825796636715947 24-25 0.46961082689705735 26-27 0.3272097009551847 28-29 0.27531755753353504 30-31 0.27791543247183553 32-33 0.32225086420188787 34-35 0.31879520036886555 36-37 0.31040520803668137 38-39 0.3359509782633026 40-41 0.341293777664713 42-43 0.3764304446761292 44-45 0.3434178263564053 46-47 0.3703769059048063 48-49 0.34641600277890927 50-51 0.36786889456500055 52-53 0.35720780401631463 54-55 0.33260151686486494 56-57 0.3699684350025578 58-59 0.35720780401631463 60-61 0.32226720303797785 62-63 0.29856772128951975 64-65 0.3056832844066886 66-67 0.3154865860606527 68-69 0.3218914098079092 70-71 0.2979795231902819 72-73 0.3056914538247336 74-75 0.303134425976658 76-77 0.3070067301299738 78-79 0.3092696589284305 80-81 0.32597611883039423 82-83 0.34379361958647386 84-85 0.33828743182416404 86-87 0.34251102095341357 88-89 0.33776458906928597 90-91 0.31075649301261504 92-93 0.294956838513643 94-95 0.30416377265032424 96-97 0.29168090187761003 98-99 0.31120581100508843 100-101 0.3350686811144459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6120387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64260097172035 #Duplication Level Percentage of deduplicated Percentage of total 1 92.59121777752229 56.14972273167944 2 3.6161441021477683 4.385847676855741 3 1.235474894759536 2.2476723316044227 4 0.6002696608889819 1.456076540828817 5 0.3008303300581412 0.9121566832953395 6 0.24434894554222472 0.8890773361426655 7 0.16766914681329223 0.7117525215827094 8 0.1198962102617308 0.5816654429538899 9 0.11100086914465203 0.6058243273547951 >10 0.8077762097485391 10.348710703464754 >50 0.11905353398040709 4.971907719759085 >100 0.07284000031806796 9.249265552217892 >500 0.01075095249086614 4.493002105368018 >1k 0.0026197969309277058 2.2508422815368836 >5k 5.378473484190948E-5 0.3175049498224295 >10k+ 5.378465781444359E-5 0.4289710955330698 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 13565 0.221636311560037 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 12739 0.20814043294974646 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9847 0.1608885189776398 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 9622 0.1572122808574033 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011437185262958044 0.0 0.0 2 0.001241751542835445 0.0 1.6338836089940064E-5 3 0.001274429215015325 0.0 1.6338836089940064E-5 4 0.001290768051105265 0.0 1.6338836089940064E-5 5 0.0014868340841845459 0.0 1.6338836089940064E-5 6 0.0015848671007241862 0.0 1.6338836089940064E-5 7 0.001764594297713527 0.0 1.6338836089940064E-5 8 0.0019769991668827478 0.0 1.6338836089940064E-5 9 0.002271098216501669 0.0 1.6338836089940064E-5 10-11 0.0034148167427974735 0.0 1.6338836089940064E-5 12-13 0.004713754211947708 0.0 2.4508254134910097E-5 14-15 0.006069877607412734 0.0 5.718592631479023E-5 16-17 0.007344306822428059 0.0 6.535534435976026E-5 18-19 0.008716769053983026 0.0 6.535534435976026E-5 20-21 0.010522210441921403 0.0 8.169418044970032E-5 22-23 0.012458362518579298 0.0 8.169418044970032E-5 24-25 0.014468039357641927 1.6338836089940064E-5 8.169418044970032E-5 26-27 0.016673782229783836 1.6338836089940064E-5 8.169418044970032E-5 28-29 0.01966378923424287 1.6338836089940064E-5 8.169418044970032E-5 30-31 0.028960586969418764 1.6338836089940064E-5 9.803301653964037E-5 32-33 0.032555130909205574 1.6338836089940064E-5 1.1437185262958044E-4 34-35 0.03631306320989179 1.6338836089940064E-5 1.1437185262958044E-4 36-37 0.04003831783839813 1.6338836089940064E-5 1.1437185262958044E-4 38-39 0.044515158927041706 1.6338836089940064E-5 1.1437185262958044E-4 40-41 0.049286099065304206 1.6338836089940064E-5 1.1437185262958044E-4 42-43 0.054522696032129996 1.6338836089940064E-5 1.1437185262958044E-4 44-45 0.06019227215533919 1.6338836089940064E-5 1.1437185262958044E-4 46-47 0.06644187695974127 1.6338836089940064E-5 1.3071068871952052E-4 48-49 0.0729774113957173 1.6338836089940064E-5 1.3071068871952052E-4 50-51 0.0797661977910874 1.6338836089940064E-5 1.3888010676449056E-4 52-53 0.08655498418645749 2.4508254134910097E-5 1.4704952480946057E-4 54-55 0.09417705122241453 3.267767217988013E-5 1.7972719698934071E-4 56-57 0.10242816344783426 3.267767217988013E-5 1.7972719698934071E-4 58-59 0.11072012276347884 3.267767217988013E-5 2.1240486916922083E-4 60-61 0.1192653340385175 3.267767217988013E-5 2.2057428721419087E-4 62-63 0.12834155748647919 3.267767217988013E-5 2.4508254134910095E-4 64-65 0.13885559851035562 3.267767217988013E-5 2.6142137743904103E-4 66-67 0.14989248228911015 3.267767217988013E-5 2.6142137743904103E-4 68-69 0.16199139041371077 3.267767217988013E-5 2.6142137743904103E-4 70-71 0.17560981029467582 3.267767217988013E-5 2.6959079548401107E-4 72-73 0.19004517198013787 3.267767217988013E-5 2.777602135289811E-4 74-75 0.20599187600391938 3.267767217988013E-5 2.777602135289811E-4 76-77 0.22477336808930545 3.267767217988013E-5 2.859296315739511E-4 78-79 0.245621722940069 3.267767217988013E-5 2.9409904961892115E-4 80-81 0.26933754352461703 3.267767217988013E-5 2.9409904961892115E-4 82-83 0.29529995407153176 3.267767217988013E-5 3.1043788570886123E-4 84-85 0.3256411726905505 3.267767217988013E-5 3.1043788570886123E-4 86-87 0.35993638964333463 3.267767217988013E-5 3.1043788570886123E-4 88-89 0.3984797039795033 3.267767217988013E-5 3.349461398437713E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2975 0.0 78.2845 9 AGAGCGG 3160 0.0 75.50388 8 CGGGAGA 3990 0.0 74.79443 4 GAGAGCG 3825 0.0 70.814384 7 GATCGGG 5525 0.0 68.02575 1 CGGAAAG 2040 0.0 66.97969 5 TCGGGAG 4615 0.0 65.489235 3 GTTCGGA 950 0.0 65.430756 2 AAGCGGC 1240 0.0 65.029205 9 ATCGGGA 5770 0.0 63.5679 2 GATCCGG 975 0.0 59.379383 1 GGTTCGG 1250 0.0 58.46436 1 AGAGCGT 7730 0.0 58.049706 8 GAGCGTC 7630 0.0 57.939217 9 TCGGGAA 1555 0.0 57.05759 3 TCGGAAA 2275 0.0 56.09994 4 GGATCGG 2320 0.0 55.432156 1 AAGGGGC 3445 0.0 54.11088 9 AAGAGCG 7930 0.0 53.239456 7 AAGCGTC 515 0.0 52.49874 9 >>END_MODULE