##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6928491 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12906287963714 33.0 33.0 33.0 33.0 33.0 2 32.092909119749166 33.0 33.0 33.0 33.0 33.0 3 32.06642615253451 33.0 33.0 33.0 33.0 33.0 4 35.81367400203017 37.0 37.0 37.0 33.0 37.0 5 35.779775422960064 37.0 37.0 37.0 33.0 37.0 6 35.8311311943683 37.0 37.0 37.0 33.0 37.0 7 35.78160381531852 37.0 37.0 37.0 33.0 37.0 8 35.78931198727111 37.0 37.0 37.0 33.0 37.0 9 35.6711817912443 37.0 37.0 37.0 33.0 37.0 10-11 35.61723497944935 37.0 37.0 37.0 33.0 37.0 12-13 35.63400948345029 37.0 37.0 37.0 33.0 37.0 14-15 37.47247885578548 40.0 37.0 40.0 33.0 40.0 16-17 37.4509074198119 40.0 37.0 40.0 33.0 40.0 18-19 37.45475645418317 40.0 37.0 40.0 33.0 40.0 20-21 37.433454629586734 40.0 37.0 40.0 33.0 40.0 22-23 37.496908706383536 40.0 37.0 40.0 33.0 40.0 24-25 37.29183035671115 40.0 37.0 40.0 33.0 40.0 26-27 37.217435441570174 40.0 37.0 40.0 33.0 40.0 28-29 37.111945082991376 40.0 37.0 40.0 33.0 40.0 30-31 37.127758555217866 40.0 37.0 40.0 33.0 40.0 32-33 37.00547723883888 40.0 37.0 40.0 33.0 40.0 34-35 36.99510030394786 40.0 37.0 40.0 33.0 40.0 36-37 36.87926433042924 40.0 37.0 40.0 33.0 40.0 38-39 36.830921841422615 40.0 37.0 40.0 33.0 40.0 40-41 36.77232264572473 40.0 37.0 40.0 33.0 40.0 42-43 36.662459329167056 40.0 37.0 40.0 33.0 40.0 44-45 36.56878193245831 40.0 37.0 40.0 33.0 40.0 46-47 36.49007684357243 40.0 37.0 40.0 33.0 40.0 48-49 36.388197444436315 40.0 37.0 40.0 33.0 40.0 50-51 35.277984556810424 37.0 35.0 38.5 30.0 38.5 52-53 35.68613483080226 37.0 37.0 38.5 33.0 40.0 54-55 36.45717595649616 38.5 37.0 40.0 33.0 40.0 56-57 36.52571202012098 40.0 37.0 40.0 33.0 40.0 58-59 36.35240068869253 37.0 37.0 40.0 33.0 40.0 60-61 36.13918716211077 37.0 37.0 40.0 33.0 40.0 62-63 35.93113904600583 37.0 37.0 40.0 33.0 40.0 64-65 35.71817081093127 37.0 37.0 40.0 33.0 40.0 66-67 35.500513243071254 37.0 37.0 40.0 27.0 40.0 68-69 35.29259278824206 37.0 37.0 40.0 27.0 40.0 70-71 34.99202250533341 37.0 33.0 37.0 27.0 40.0 72-73 34.78208537760965 37.0 33.0 37.0 27.0 40.0 74-75 34.55945818505069 37.0 33.0 37.0 27.0 40.0 76-77 34.300794935001 37.0 33.0 37.0 27.0 37.0 78-79 34.007013864923834 37.0 33.0 37.0 27.0 37.0 80-81 33.73171315370115 37.0 33.0 37.0 27.0 37.0 82-83 33.47546680799614 37.0 33.0 37.0 27.0 37.0 84-85 33.31946472904417 37.0 33.0 37.0 27.0 37.0 86-87 33.15415275851552 37.0 33.0 37.0 27.0 37.0 88-89 32.928377189203246 37.0 33.0 37.0 27.0 37.0 90-91 32.84956731559585 37.0 33.0 37.0 27.0 37.0 92-93 32.70520471196397 37.0 33.0 37.0 27.0 37.0 94-95 32.57622713228609 33.0 33.0 37.0 27.0 37.0 96-97 32.43605642267559 33.0 33.0 37.0 22.0 37.0 98-99 32.3066185696135 33.0 33.0 37.0 22.0 37.0 100-101 31.07034381656843 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.26663406432727044 2306 2 0.23631087172410759 2306 3 0.22774295307579706 2306 4 0.35211569962076794 2306 5 0.33152365192328404 2306 6 0.3318887950013334 2306 7 0.36126139320577266 2306 8 0.2878300022585023 2306 9 0.3384659539812347 2306 10-11 0.40165971157830427 2306 12-13 0.34677295656964446 2306 14-15 0.526920795631078 2306 16-17 0.47057475093266277 2306 18-19 0.5444523848181291 2306 20-21 0.5139563756119543 2306 22-23 0.4511637930129311 2306 24-25 0.5701963440707658 2306 26-27 0.5014489508351261 2306 28-29 0.46490186754873264 2306 30-31 0.5081120302792002 2306 32-33 0.5121404974627026 2306 34-35 0.41845771393961684 2306 36-37 0.44304460532331547 2306 38-39 0.4994658319237004 2306 40-41 0.40616910527905503 2306 42-43 0.42643702264175687 2306 44-45 0.32914735374117043 2306 46-47 0.3594585134332391 2306 48-49 0.43916432732186195 2306 50-51 0.4557669388837198 2306 52-53 0.4298235518916229 2306 54-55 0.4653228002487779 2306 56-57 0.46253201931590837 2306 58-59 0.4916199980553415 2306 60-61 0.41664908730202654 2306 62-63 0.3047608661839263 2306 64-65 0.32142576167662185 2306 66-67 0.41113273042804366 2306 68-69 0.39106239497304784 2306 70-71 0.43584931546588024 2306 72-73 0.3515214440649146 2306 74-75 0.4042097280400938 2306 76-77 0.3856509471460683 2306 78-79 0.33863805288233095 2306 80-81 0.34256624724184803 2306 82-83 0.4075417796789935 2306 84-85 0.3686867604122597 2306 86-87 0.35533737540914956 2306 88-89 0.46360654451338945 2306 90-91 0.26929380508782685 2306 92-93 0.33144827615982564 2306 94-95 0.3046677236320008 2306 96-97 0.33985714116229815 2306 98-99 0.3516717435052641 2306 100-101 0.16939321670796303 2307 1 0.24044611783254766 2307 2 0.2310434572970479 2307 3 0.19548371241400986 2307 4 0.2744153986616027 2307 5 0.3214164132316384 2307 6 0.3233744330763457 2307 7 0.33415276538981686 2307 8 0.291531234986401 2307 9 0.3862770757177785 2307 10-11 0.3555780123050667 2307 12-13 0.2604026222096607 2307 14-15 0.42649162764511317 2307 16-17 0.45620760207035005 2307 18-19 0.45556537936717234 2307 20-21 0.41303332755550315 2307 22-23 0.37320907867894704 2307 24-25 0.3765091731615655 2307 26-27 0.4265928899140832 2307 28-29 0.37546612067852436 2307 30-31 0.3278729260565001 2307 32-33 0.34014149106506153 2307 34-35 0.29853059232673473 2307 36-37 0.36002770022406594 2307 38-39 0.23235174569138195 2307 40-41 0.38881315363294533 2307 42-43 0.3150146467594226 2307 44-45 0.3695925771471167 2307 46-47 0.2901740030755704 2307 48-49 0.2079957678000781 2307 50-51 0.24508042575633482 2307 52-53 0.31026811431745926 2307 54-55 0.2479906652084125 2307 56-57 0.22077069923202686 2307 58-59 0.23045805899557337 2307 60-61 0.29895047077602754 2307 62-63 0.28095430307791247 2307 64-65 0.2859747120508729 2307 66-67 0.2690205365177931 2307 68-69 0.26514429893690306 2307 70-71 0.2270748898522541 2307 72-73 0.13480263459668862 2307 74-75 0.1551991406313462 2307 76-77 0.1633111628601256 2307 78-79 0.27928830805263516 2307 80-81 0.22934960888005662 2307 82-83 0.373009385790283 2307 84-85 0.36166442248298125 2307 86-87 0.3442088371666543 2307 88-89 0.437769770352169 2307 90-91 0.35213823702340363 2307 92-93 0.42174212446062853 2307 94-95 0.29866886009134674 2307 96-97 0.41392241401381824 2307 98-99 0.3533102278992075 2307 100-101 0.2937144700968197 2201 1 0.2909504595465435 2201 2 0.28268762137685144 2201 3 0.2471202776780359 2201 4 0.3723425248310335 2201 5 0.4255904783546782 2201 6 0.3803722003305552 2201 7 0.3673462923860882 2201 8 0.3459581091482349 2201 9 0.4319492829897271 2201 10-11 0.40015100338298026 2201 12-13 0.38387073360117796 2201 14-15 0.5370793901085094 2201 16-17 0.49858293251493535 2201 18-19 0.4607061563342114 2201 20-21 0.402825862075197 2201 22-23 0.43569307218908193 2201 24-25 0.4934897362219388 2201 26-27 0.5296263609165877 2201 28-29 0.4936733581943429 2201 30-31 0.3973562006474225 2201 32-33 0.4621658868256304 2201 34-35 0.40707605431989435 2201 36-37 0.46757937959203133 2201 38-39 0.4080792877772481 2201 40-41 0.36168276923378784 2201 42-43 0.4394461455130383 2201 44-45 0.5711224324787239 2201 46-47 0.42321858140956437 2201 48-49 0.4464322995818222 2201 50-51 0.4513935575192036 2201 52-53 0.4181094391709905 2201 54-55 0.5776595883671689 2201 56-57 0.5535066350410247 2201 58-59 0.47757220092601926 2201 60-61 0.47699090241251696 2201 62-63 0.5327389265180003 2201 64-65 0.45616960386570327 2201 66-67 0.4436645943329154 2201 68-69 0.43531010153510863 2201 70-71 0.4314579224090309 2201 72-73 0.38726681775664673 2201 74-75 0.518474479565235 2201 76-77 0.37043635869288494 2201 78-79 0.3530200330491198 2201 80-81 0.5101982185045486 2201 82-83 0.49325209671530246 2201 84-85 0.47419742241827834 2201 86-87 0.5641375626493499 2201 88-89 0.732838198281847 2201 90-91 0.5048996644846895 2201 92-93 0.46561085966742866 2201 94-95 0.47810373378774074 2201 96-97 0.5202589952472394 2201 98-99 0.5364345641159289 2201 100-101 0.3982925191364899 1101 1 0.2549487996854509 1101 2 0.20674020646077196 1101 3 0.20479695698617206 1101 4 0.2667829527158432 1101 5 0.30242611146548626 1101 6 0.3631858645238282 1101 7 0.3818106128590628 1101 8 0.3205374577097899 1101 9 0.3170435449896729 1101 10-11 0.273580712757564 1101 12-13 0.3393121083483308 1101 14-15 0.47002576119441386 1101 16-17 0.3942385776469095 1101 18-19 0.4120626186340459 1101 20-21 0.39849469768406465 1101 22-23 0.3867787553880504 1101 24-25 0.43857999116890767 1101 26-27 0.42540027471645914 1101 28-29 0.37703916862081854 1101 30-31 0.35944165426079877 1101 32-33 0.4740727342517417 1101 34-35 0.44216550357827344 1101 36-37 0.46906216744567786 1101 38-39 0.4359721910867762 1101 40-41 0.2986267460698855 1101 42-43 0.39024049742213407 1101 44-45 0.4494184296324377 1101 46-47 0.33380283397654154 1101 48-49 0.4372337422634942 1101 50-51 0.39177618473422626 1101 52-53 0.4159678939458402 1101 54-55 0.4356974550178663 1101 56-57 0.4251735976021749 1101 58-59 0.38936479030219573 1101 60-61 0.4987079887859025 1101 62-63 0.5021988014437611 1101 64-65 0.43989581733610095 1101 66-67 0.4483565360923265 1101 68-69 0.3557349489711896 1101 70-71 0.36901247572600226 1101 72-73 0.4037274456090856 1101 74-75 0.5585032098499383 1101 76-77 0.4741087300625608 1101 78-79 0.37147019479288446 1101 80-81 0.4576647556906437 1101 82-83 0.5037517876303426 1101 84-85 0.5186243211517692 1101 86-87 0.5149252301951392 1101 88-89 0.5740223965183233 1101 90-91 0.6099739339996475 1101 92-93 0.57706402598793 1101 94-95 0.5461708369522498 1101 96-97 0.4810557214421678 1101 98-99 0.43678897797035177 1101 100-101 0.37559279570870885 2304 1 0.2680384375949032 2304 2 0.21328911570592624 2304 3 0.23300153849793048 2304 4 0.3785123275068969 2304 5 0.3756571574783507 2304 6 0.3506398809635769 2304 7 0.3860922129291495 2304 8 0.38333510685563255 2304 9 0.42499229976999686 2304 10-11 0.3886676040531043 2304 12-13 0.36598545582268827 2304 14-15 0.4509317540818145 2304 16-17 0.43048217408789924 2304 18-19 0.4038670454233255 2304 20-21 0.4299992900817955 2304 22-23 0.44436118762288856 2304 24-25 0.47541521430229494 2304 26-27 0.5157692189126593 2304 28-29 0.45331606389070345 2304 30-31 0.41269564132775116 2304 32-33 0.38674134600717736 2304 34-35 0.40958366717313766 2304 36-37 0.47233198425863776 2304 38-39 0.3649599504900891 2304 40-41 0.3077439229063472 2304 42-43 0.3446784303246133 2304 44-45 0.3725708583309384 2304 46-47 0.32964426468927144 2304 48-49 0.33998639499397143 2304 50-51 0.3590529112393668 2304 52-53 0.3521059802181057 2304 54-55 0.4764824010743425 2304 56-57 0.4100057378804607 2304 58-59 0.4936018963862878 2304 60-61 0.4260323526813252 2304 62-63 0.38900800131209934 2304 64-65 0.43162033853057125 2304 66-67 0.42749906628800716 2304 68-69 0.4622638254553735 2304 70-71 0.4859139905505714 2304 72-73 0.34377266103212634 2304 74-75 0.3680380150007423 2304 76-77 0.5022317634219391 2304 78-79 0.45698067914124607 2304 80-81 0.2938104267140602 2304 82-83 0.4570001545738407 2304 84-85 0.5189272119886894 2304 86-87 0.40819066363387435 2304 88-89 0.4760456731694589 2304 90-91 0.4771607224259924 2304 92-93 0.5049576004959633 2304 94-95 0.4382994112058327 2304 96-97 0.419369343011077 2304 98-99 0.3919946132955161 2304 100-101 0.40457673858119847 2202 1 0.20970015278603427 2202 2 0.18669023252231653 2202 3 0.22510223525183193 2202 4 0.28366910452496086 2202 5 0.275455994546725 2202 6 0.2210260664946233 2202 7 0.3057615430462377 2202 8 0.24611124399524442 2202 9 0.2927074968091645 2202 10-11 0.30092575772916774 2202 12-13 0.3149394381392696 2202 14-15 0.3545210565297694 2202 16-17 0.333699894162514 2202 18-19 0.3623481839966445 2202 20-21 0.37230883563980655 2202 22-23 0.2884441004503131 2202 24-25 0.3767739878735412 2202 26-27 0.4985156915406108 2202 28-29 0.3962464653592974 2202 30-31 0.3632935135235087 2202 32-33 0.41323292258654476 2202 34-35 0.3981085856585551 2202 36-37 0.43561198946138546 2202 38-39 0.3235005067698964 2202 40-41 0.29584730084729927 2202 42-43 0.30559377339536553 2202 44-45 0.38829194097476716 2202 46-47 0.20471994097214719 2202 48-49 0.32022660807000847 2202 50-51 0.2875208655852859 2202 52-53 0.33199685560942527 2202 54-55 0.3946285981933215 2202 56-57 0.35996013996392406 2202 58-59 0.36244130008083175 2202 60-61 0.3462238048086803 2202 62-63 0.4052617740408522 2202 64-65 0.4225960157002788 2202 66-67 0.4328435559608508 2202 68-69 0.3733093849425444 2202 70-71 0.39705976199046233 2202 72-73 0.4296655377063914 2202 74-75 0.5138372422749455 2202 76-77 0.48582493836718044 2202 78-79 0.4897377937464711 2202 80-81 0.4450555380557475 2202 82-83 0.4602871214033897 2202 84-85 0.44237089671420193 2202 86-87 0.33230760122754077 2202 88-89 0.5869659677157415 2202 90-91 0.5312950566964076 2202 92-93 0.47663116966733554 2202 94-95 0.45330496868285763 2202 96-97 0.5005588352409589 2202 98-99 0.47392217099129397 2202 100-101 0.5649957516560491 1102 1 0.0562832316365558 1102 2 0.06237635733634761 1102 3 0.03641399627289843 1102 4 0.1274548299967293 1102 5 0.12356303393283241 1102 6 0.11766493580791604 1102 7 0.14334278179026683 1102 8 0.06584344977396484 1102 9 0.07314755631899317 1102 10-11 0.13416757512796096 1102 12-13 0.1828613902181928 1102 14-15 0.19323865891016112 1102 16-17 0.13199583809799265 1102 18-19 0.21829965608183954 1102 20-21 0.1872396358903714 1102 22-23 0.19297242634491596 1102 24-25 0.13537991145039285 1102 26-27 0.21104652311213812 1102 28-29 0.1692835219719342 1102 30-31 0.1173290236636717 1102 32-33 0.1305539037156649 1102 34-35 0.1200303224058672 1102 36-37 0.17331117120125583 1102 38-39 0.10944082076252926 1102 40-41 0.054088139466038854 1102 42-43 0.05240931250741454 1102 44-45 0.15402485147512124 1102 46-47 0.12152510501568514 1102 48-49 0.1856536498051895 1102 50-51 0.2048981265672012 1102 52-53 0.19442638344592922 1102 54-55 0.3026820004327746 1102 56-57 0.3162062248437252 1102 58-59 0.3054851102758036 1102 60-61 0.24195632379701948 1102 62-63 0.2642773170680286 1102 64-65 0.3164859812472187 1102 66-67 0.34729181764748773 1102 68-69 0.34870187574202305 1102 70-71 0.3861752384605879 1102 72-73 0.34909040654986256 1102 74-75 0.3996057803690647 1102 76-77 0.3424038084634873 1102 78-79 0.3715824026259611 1102 80-81 0.39141153993499955 1102 82-83 0.32420782064385634 1102 84-85 0.42346135556628184 1102 86-87 0.3395309979858254 1102 88-89 0.4496176786232624 1102 90-91 0.37342370754598875 1102 92-93 0.3983297445635827 1102 94-95 0.34331945462757574 1102 96-97 0.3922069050867023 1102 98-99 0.347112406681525 1102 100-101 0.31838771391429077 2305 1 0.20425434270025988 2305 2 0.1565769661378411 2305 3 0.2054460597785024 2305 4 0.2883401589513994 2305 5 0.26426877711428176 2305 6 0.2919691259944841 2305 7 0.2878416829768611 2305 8 0.3168147629024034 2305 9 0.24727817428153998 2305 10-11 0.3064406863560052 2305 12-13 0.25503725162035806 2305 14-15 0.31656024266312954 2305 16-17 0.2938166299863312 2305 18-19 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33383.0 26 39970.0 27 48007.0 28 59114.0 29 74096.0 30 93046.0 31 121098.0 32 165323.0 33 238675.0 34 364531.0 35 617919.0 36 1217242.0 37 2421736.0 38 1111094.0 39 866.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.38748168058052 26.794550577806103 21.46061290442556 16.35735483718781 2 31.89984633148989 24.53255544076238 29.9508752738396 13.616722953908129 3 36.12921395202644 21.249269357206472 25.338626988935886 17.282889701831202 4 30.925368704169635 20.879248679278362 30.178558151819995 18.01682446473201 5 29.848951496922215 19.628507686968256 32.57667546308507 17.945865353024466 6 26.44878734471621 19.81019446986714 35.4370110511801 18.304007134236553 7 32.06513044980006 19.81912997595786 28.014358170899666 20.101381403342412 8 29.83123233114483 27.390287130376876 23.619518767856878 19.158961770621413 9 27.5513248195781 27.01716291034441 25.53640692497338 19.89510534510411 10-11 23.37159465955054 29.46377319544103 29.92952553468548 17.235106610322955 12-13 23.564826154218967 27.463598945848815 30.174632930531182 18.796941969401036 14-15 22.36005230509551 27.690801478039084 31.213245803360696 18.735900413504712 16-17 22.064822999634384 27.966778010209513 32.06747545968261 17.9009235304735 18-19 21.82565412116599 28.096972762826546 31.955910375459723 18.12146274054774 20-21 21.712478743079885 28.863249545504306 31.196394924688427 18.227876786727382 22-23 22.259279148138706 28.76074207307346 30.480897764688862 18.499081014098973 24-25 21.626655830232288 29.132742985504382 30.527056496951456 18.713544687311874 26-27 21.484901563638488 29.469724456966688 30.370866975782217 18.674507003612607 28-29 21.668837191125444 29.35770448131582 30.114668101540744 18.858790226017994 30-31 21.87003945744868 29.103253685289808 30.16775585552548 18.85895100173603 32-33 21.278255356093894 29.487494633896137 30.355505602650478 18.878744407359488 34-35 22.065030367028903 28.969548261258172 30.224549852282696 18.74087151943023 36-37 21.388677471711343 28.801706582463066 30.755160278433102 19.054455667392485 38-39 21.24691624079869 29.240286574897585 30.55831212321075 18.954485061092974 40-41 21.374189642486776 29.64627423830739 29.775294161949837 19.204241957255995 42-43 21.509717397427472 29.04175809145609 30.199143765553803 19.24938074556264 44-45 21.05515293682105 29.166969925224222 30.462121141347165 19.31575599660756 46-47 21.359548999860117 29.33481420105981 29.90729340153033 19.398343397549745 48-49 20.989732469466603 29.56558353081555 29.792908368876752 19.651775630841094 50-51 20.9372006257228 29.231425076793748 29.887818289751383 19.943556007732074 52-53 21.701661761182343 29.090519150800258 29.33941972644984 19.86839936156756 54-55 21.779507208353017 29.243113519575793 29.261006594441692 19.7163726776295 56-57 21.7165752611647 29.219109017477724 29.42147630033258 19.642839421025002 58-59 21.54035841699931 29.248908194466257 29.5030350948047 19.70769829372973 60-61 20.820062737324886 29.35081986869242 29.422131371877214 20.40698602210548 62-63 21.0054557199787 29.2048162176358 29.534860927206008 20.25486713517949 64-65 20.683335079193142 29.377137788444212 29.753613694529164 20.18591343783348 66-67 20.60296810684617 29.068194695368028 29.717485262779437 20.611351935006365 68-69 20.448991430584666 29.13258743980716 30.3509176882591 20.06750344134907 70-71 20.27302136062606 30.054116384973366 29.632340113806556 20.04052214059402 72-73 20.29268212169346 30.394639894516757 29.1968702506588 20.115807733130982 74-75 20.41811785649755 30.378554956611982 29.10778899477861 20.09553819211186 76-77 20.470405175891752 30.317395612332533 29.128029871472137 20.084169340303575 78-79 20.31959351445143 30.26536725577805 29.39568677400238 20.019352455768143 80-81 20.160458524091187 30.550974840852398 29.29114575342224 19.997420881634177 82-83 20.086682157745102 30.41196438857627 29.26104717005864 20.240306283619987 84-85 20.208336863619714 30.017789746715895 29.460583827537217 20.313289562127174 86-87 20.343607158949577 29.987664225341014 29.55481135041899 20.113917265290414 88-89 20.601287297583887 30.270552924748422 29.08916993905312 20.03898983861457 90-91 20.654354297046147 30.08358717846324 29.0581677222512 20.203890802239417 92-93 20.388157309948802 30.231270374339168 29.022577496682633 20.357994819029397 94-95 20.145029607563238 30.269998940919447 28.99795126188036 20.587020189636952 96-97 20.244391460630563 30.556843959772834 28.847138801668265 20.351625777928334 98-99 20.538834178994673 30.48533475114274 28.684490928669504 20.291340141193082 100-101 20.64776128870707 30.315251193980803 28.37795877473246 20.659028742579665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3907.0 1 2416.5 2 753.0 3 498.0 4 405.5 5 460.5 6 626.5 7 923.0 8 1281.0 9 1691.5 10 2105.5 11 2382.5 12 2442.5 13 2265.0 14 2009.5 15 1770.5 16 1561.5 17 1438.0 18 1449.5 19 1629.0 20 1941.0 21 2396.5 22 3301.0 23 4796.5 24 7148.0 25 11077.0 26 17795.5 27 27022.5 28 38331.0 29 52265.5 30 68765.0 31 87933.0 32 113207.5 33 148164.5 34 190160.5 35 236802.5 36 291480.5 37 350725.0 38 405908.5 39 440398.0 40 457255.0 41 451638.5 42 424736.0 43 402172.0 44 373750.0 45 345257.0 46 328829.0 47 302221.0 48 260647.0 49 221323.5 50 181832.0 51 146329.5 52 118647.0 53 94224.5 54 76870.0 55 61193.0 56 43860.5 57 30098.5 58 21512.0 59 15778.0 60 11526.5 61 8314.5 62 5852.5 63 3965.5 64 2808.0 65 1982.0 66 1368.5 67 1004.5 68 772.5 69 591.0 70 467.5 71 359.5 72 277.0 73 233.0 74 178.5 75 131.0 76 112.0 77 106.0 78 90.5 79 77.0 80 76.5 81 65.0 82 42.5 83 36.5 84 33.0 85 27.0 86 22.5 87 23.0 88 22.0 89 18.5 90 14.5 91 8.0 92 7.0 93 6.0 94 3.5 95 3.5 96 4.0 97 2.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26663814674797154 2 0.2527390163312617 3 0.21733448163532293 4 0.2197448188934647 5 0.2339326124548621 6 0.23769966649303578 7 0.21356742759714922 8 0.20912201516895956 9 0.1901135470912786 10-11 0.17852372183206994 12-13 0.2064302313447474 14-15 0.20292297413679253 16-17 0.20430134065267605 18-19 0.19660125126813327 20-21 0.19750332359528217 22-23 0.16389571697502386 24-25 0.47967154752744867 26-27 0.3332688171204956 28-29 0.2834455583474093 30-31 0.28735694395792677 32-33 0.33016568831510357 34-35 0.3256625432579764 36-37 0.3183449325401448 38-39 0.34306171430402377 40-41 0.34828651722287 42-43 0.3845426081956374 44-45 0.3509638678898479 46-47 0.3786033639936892 48-49 0.35352575329895064 50-51 0.3763229251506569 52-53 0.3656351722185971 54-55 0.3404060133728975 56-57 0.37799717138984523 58-59 0.3643939206964402 60-61 0.3301296054220176 62-63 0.3066324254444438 64-65 0.31283146647661086 66-67 0.32240786630162327 68-69 0.32862855706964184 70-71 0.3053478744505838 72-73 0.31192939414946197 74-75 0.31052937789772694 76-77 0.3132067285647048 78-79 0.31571088134487 80-81 0.3324028276864327 82-83 0.35075458710994933 84-85 0.34570298207791567 86-87 0.3490226082418235 88-89 0.34454111292054795 90-91 0.3182366838608869 92-93 0.30172515198475397 94-95 0.3111283539229538 96-97 0.2990694510536277 98-99 0.31842431490493384 100-101 0.3413153022786636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6928491.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.75061897597054 #Duplication Level Percentage of deduplicated Percentage of total 1 91.64681086805034 60.25834541748014 2 4.470073273257843 5.878201691692919 3 1.2991350287598835 2.5625679682298292 4 0.6267417587220335 1.6483463429624838 5 0.3530425133952763 1.1606381890285895 6 0.23550180841743976 0.9290633804044256 7 0.1674298398292255 0.7706030922673428 8 0.17218308369460375 0.9056915464089231 9 0.08484933464478989 0.5021006645334778 >10 0.781748843481315 10.048245132847015 >50 0.09412361327265564 4.297059439246244 >100 0.06268690860257563 8.085489926197145 >500 0.004673129545264752 2.0794206025672244 >1k 9.999963266773095E-4 0.8742266061342464 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.082568051253873E-4 1.4433157234381916E-5 0.0 2 9.381552202348246E-4 1.4433157234381916E-5 1.4433157234381916E-5 3 0.0010247541636411162 1.4433157234381916E-5 1.4433157234381916E-5 4 0.0011402194215161714 1.4433157234381916E-5 1.4433157234381916E-5 5 0.0012845509938599906 1.4433157234381916E-5 1.4433157234381916E-5 6 0.0014721820379069554 1.4433157234381916E-5 1.4433157234381916E-5 7 0.0017608451825945938 1.4433157234381916E-5 1.4433157234381916E-5 8 0.002078374641750996 1.4433157234381916E-5 2.8866314468763832E-5 9 0.00241033725814178 1.4433157234381916E-5 2.8866314468763832E-5 10-11 0.0043660300634005295 1.4433157234381916E-5 5.0516050320336706E-5 12-13 0.006292856554190516 1.4433157234381916E-5 5.7732628937527664E-5 14-15 0.008631028026160385 1.4433157234381916E-5 6.494920755471863E-5 16-17 0.011127964227708457 1.4433157234381916E-5 7.216578617190958E-5 18-19 0.012982624932326534 1.4433157234381916E-5 8.65989434062915E-5 20-21 0.0146063551211945 1.4433157234381916E-5 8.65989434062915E-5 22-23 0.01626616820314842 1.4433157234381916E-5 9.381552202348246E-5 24-25 0.018561040203415147 1.4433157234381916E-5 1.0103210064067341E-4 26-27 0.020834262467830295 1.4433157234381916E-5 1.0824867925786436E-4 28-29 0.02393017469460522 1.4433157234381916E-5 1.226818364922463E-4 30-31 0.0438984477283726 1.4433157234381916E-5 1.5154815096101012E-4 32-33 0.04873355540189054 1.4433157234381916E-5 1.587647295782011E-4 34-35 0.052854221792306574 1.4433157234381916E-5 1.587647295782011E-4 36-37 0.057350150270816545 1.4433157234381916E-5 1.587647295782011E-4 38-39 0.06241618846008459 1.4433157234381916E-5 1.587647295782011E-4 40-41 0.06781418926574344 1.4433157234381916E-5 1.8041446542977396E-4 42-43 0.0736668345242853 1.4433157234381916E-5 1.8763104404696492E-4 44-45 0.08043598526721042 1.4433157234381916E-5 1.8763104404696492E-4 46-47 0.0877102965133389 1.4433157234381916E-5 1.9484762266415586E-4 48-49 0.09597327903002256 1.4433157234381916E-5 2.3093051575011066E-4 50-51 0.10542699701854272 1.4433157234381916E-5 2.597968302188745E-4 52-53 0.1145415358120549 1.4433157234381916E-5 2.597968302188745E-4 54-55 0.12489732612772392 1.4433157234381916E-5 2.597968302188745E-4 56-57 0.1364077690221435 1.4433157234381916E-5 2.597968302188745E-4 58-59 0.14858213714934465 1.4433157234381916E-5 2.597968302188745E-4 60-61 0.16212043863519487 1.4433157234381916E-5 2.6701340883606545E-4 62-63 0.17741236872502253 1.4433157234381916E-5 2.8144656607044733E-4 64-65 0.19415483111690554 1.4433157234381916E-5 3.103128805392112E-4 66-67 0.21347361207512572 1.4433157234381916E-5 3.3917919500797505E-4 68-69 0.23484190136062816 1.4433157234381916E-5 3.608289308595479E-4 70-71 0.2579782524073424 1.4433157234381916E-5 3.608289308595479E-4 72-73 0.28472289276265206 1.4433157234381916E-5 3.608289308595479E-4 74-75 0.31347374197354083 1.4433157234381916E-5 3.824786667111208E-4 76-77 0.3477019743548776 1.4433157234381916E-5 4.185615597970756E-4 78-79 0.38585602550396614 1.4433157234381916E-5 4.329947170314575E-4 80-81 0.42891013353412744 1.4433157234381916E-5 4.4742787426583944E-4 82-83 0.47758595630708045 1.4433157234381916E-5 4.546444528830304E-4 84-85 0.5317391622504813 1.4433157234381916E-5 4.618610315002213E-4 86-87 0.5924305884210573 1.4433157234381916E-5 4.7629418873460324E-4 88-89 0.6608509702906449 1.4433157234381916E-5 4.7629418873460324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1535 0.0 52.837128 9 AGAGCGG 1640 0.0 52.06013 8 CGGGAGA 1990 0.0 51.73305 4 GATCGGG 2905 0.0 49.823902 1 GAGAGCG 2165 0.0 45.573612 7 ATCGGGA 3110 0.0 43.633858 2 TTGTGTT 55195 0.0 39.945095 1 GGCGCCG 1380 0.0 39.417873 56-57 CGCGCAA 85 3.6712845E-7 39.080772 1 GGGCGCC 2015 0.0 35.720325 54-55 CGGAAAG 1090 0.0 35.25315 5 CGCCGTA 1660 0.0 35.19712 58-59 TGTGTTT 97455 0.0 35.113598 1 AGCGGCG 1435 0.0 34.708134 10-11 TCGGGAG 3075 0.0 34.56 3 CGCCGGA 855 0.0 34.44575 58-59 TCGGGAA 1015 0.0 34.121464 3 TCGCCGT 1380 0.0 33.9097 56-57 CCGGATC 915 0.0 33.216526 60-61 GTCGCCG 1770 0.0 33.14824 56-57 >>END_MODULE