##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_35008-2.342000000358b0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5863258 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.604642674772286 33.0 33.0 33.0 27.0 33.0 2 31.385546738690334 33.0 33.0 33.0 27.0 33.0 3 31.310628664131784 33.0 33.0 33.0 27.0 33.0 4 34.87627851273132 37.0 37.0 37.0 33.0 37.0 5 34.872496997403154 37.0 37.0 37.0 33.0 37.0 6 35.12149030453717 37.0 37.0 37.0 33.0 37.0 7 34.860843749328446 37.0 37.0 37.0 33.0 37.0 8 35.01481309538144 37.0 37.0 37.0 33.0 37.0 9 34.69243226206317 37.0 37.0 37.0 33.0 37.0 10-11 34.66847425100516 37.0 37.0 37.0 33.0 37.0 12-13 34.65920756002892 37.0 37.0 37.0 33.0 37.0 14-15 36.335470739987905 40.0 37.0 40.0 33.0 40.0 16-17 36.278919416474594 40.0 37.0 40.0 33.0 40.0 18-19 36.28446556504933 40.0 37.0 40.0 33.0 40.0 20-21 36.262365394802686 40.0 37.0 40.0 33.0 40.0 22-23 36.58110490788569 40.0 37.0 40.0 33.0 40.0 24-25 36.19082564676499 40.0 37.0 40.0 27.0 40.0 26-27 36.10411890454078 40.0 37.0 40.0 27.0 40.0 28-29 35.872895581262156 40.0 37.0 40.0 27.0 40.0 30-31 35.86961370282529 40.0 37.0 40.0 27.0 40.0 32-33 35.738972257403645 40.0 37.0 40.0 27.0 40.0 34-35 35.72595082802087 40.0 37.0 40.0 27.0 40.0 36-37 35.60660796437749 40.0 37.0 40.0 27.0 40.0 38-39 35.49756995172309 38.5 37.0 40.0 27.0 40.0 40-41 35.48125342599626 37.0 37.0 40.0 27.0 40.0 42-43 35.34987774715013 37.0 37.0 40.0 27.0 40.0 44-45 35.21636886863925 37.0 37.0 40.0 24.5 40.0 46-47 35.1326040914454 37.0 37.0 40.0 22.0 40.0 48-49 35.02790121123785 37.0 37.0 40.0 22.0 40.0 50-51 34.02497024691733 37.0 35.0 38.5 22.0 38.5 52-53 34.44129893311875 37.0 35.0 38.5 24.5 40.0 54-55 35.131351119121824 37.0 37.0 40.0 24.5 40.0 56-57 35.28755258595136 37.0 37.0 40.0 27.0 40.0 58-59 35.0045134462785 37.0 37.0 40.0 24.5 40.0 60-61 34.847923799362064 37.0 37.0 40.0 22.0 40.0 62-63 34.605783081692806 37.0 33.0 40.0 22.0 40.0 64-65 34.42845249177164 37.0 33.0 40.0 22.0 40.0 66-67 34.26377817588788 37.0 33.0 40.0 22.0 40.0 68-69 34.170912230026374 37.0 33.0 40.0 22.0 40.0 70-71 33.83059435556136 37.0 33.0 38.5 22.0 40.0 72-73 33.806859087558486 37.0 33.0 37.0 22.0 40.0 74-75 33.69590874220442 37.0 33.0 37.0 27.0 40.0 76-77 33.399990159054916 37.0 33.0 37.0 22.0 40.0 78-79 33.005155410183214 37.0 33.0 37.0 22.0 37.0 80-81 32.609699163843715 37.0 33.0 37.0 22.0 37.0 82-83 32.25695722412351 37.0 33.0 37.0 18.5 37.0 84-85 32.05021056552518 37.0 33.0 37.0 15.0 37.0 86-87 31.850119677489886 37.0 33.0 37.0 15.0 37.0 88-89 31.587097224785264 33.0 33.0 37.0 15.0 37.0 90-91 31.479153143184217 33.0 33.0 37.0 15.0 37.0 92-93 31.308948284383867 33.0 33.0 37.0 15.0 37.0 94-95 31.153690576126788 33.0 33.0 37.0 15.0 37.0 96-97 31.001153283720413 33.0 33.0 37.0 15.0 37.0 98-99 30.848584609444103 33.0 33.0 37.0 15.0 37.0 100-101 29.60965294380701 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15272591498301225 2306 2 0.09123786349265472 2306 3 0.01799222973182424 2306 4 0.14256909451259503 2306 5 0.1927398399275404 2306 6 0.0976384223610296 2306 7 0.3252526151766304 2306 8 0.057427638684622195 2306 9 0.28280362257770975 2306 10-11 0.19311523426535615 2306 12-13 0.1567710627460528 2306 14-15 0.42370733634053437 2306 16-17 0.2680266427535898 2306 18-19 0.3920486583328895 2306 20-21 0.252741105673735 2306 22-23 0.11665725030518814 2306 24-25 0.2671684135565755 2306 26-27 0.14976324768367277 2306 28-29 0.13213143315425668 2306 30-31 0.14660404398377835 2306 32-33 0.06110393501985101 2306 34-35 -0.03497115738155543 2306 36-37 0.008354196071941544 2306 38-39 -0.04474711530274078 2306 40-41 -0.006743391745786198 2306 42-43 -0.03760759862232277 2306 44-45 -0.045778325362881844 2306 46-47 -0.04536985532958937 2306 48-49 0.05249790442484681 2306 50-51 0.09012405476838836 2306 52-53 0.1041590393730587 2306 54-55 0.1370801362792733 2306 56-57 0.06042987359348473 2306 58-59 0.255799112744711 2306 60-61 0.198483215696605 2306 62-63 0.18518280318456704 2306 64-65 0.12177886531420512 2306 66-67 0.0823155951691632 2306 68-69 0.15884830712230524 2306 70-71 0.2619510797340112 2306 72-73 -0.03275758328647527 2306 74-75 0.12168500533501714 2306 76-77 0.021845721480133307 2306 78-79 0.012603268292458836 2306 80-81 -0.065270146317296 2306 82-83 -0.04218962983498642 2306 84-85 5.466716112962899E-4 2306 86-87 0.16816821060221443 2306 88-89 0.2543055121583073 2306 90-91 0.04975340827864727 2306 92-93 0.022466513911734154 2306 94-95 -0.03630429254424428 2306 96-97 -0.016159432569452292 2306 98-99 -0.06829803124931288 2306 100-101 -0.09158763354070842 2307 1 0.24208949939494318 2307 2 0.2349706346250322 2307 3 0.1854399661452888 2307 4 0.365784097461507 2307 5 0.3811633519756228 2307 6 0.3836025584297005 2307 7 0.5104755587527237 2307 8 0.23545607976527805 2307 9 0.3620862253286745 2307 10-11 0.3540132435312344 2307 12-13 0.26187368467639516 2307 14-15 0.5353167888418184 2307 16-17 0.48687576792357135 2307 18-19 0.45510267191245646 2307 20-21 0.3126599231870628 2307 22-23 0.346429472344731 2307 24-25 0.4760031000874321 2307 26-27 0.4058188967960277 2307 28-29 0.4998776194430832 2307 30-31 0.4720621138677856 2307 32-33 0.5419143634715908 2307 34-35 0.5668311075576895 2307 36-37 0.4663654452244188 2307 38-39 0.37865242356816964 2307 40-41 0.46040527046373114 2307 42-43 0.4394051430916903 2307 44-45 0.40527371513023525 2307 46-47 0.3967524654163057 2307 48-49 0.3475480504969255 2307 50-51 0.43430194096660557 2307 52-53 0.4670925107107564 2307 54-55 0.4045797283845971 2307 56-57 0.41243990988361645 2307 58-59 0.36770899736463036 2307 60-61 0.3494783850582195 2307 62-63 0.46937884069588875 2307 64-65 0.47961622854982977 2307 66-67 0.38647604803213653 2307 68-69 0.3395746020942596 2307 70-71 0.3818175119167293 2307 72-73 0.1252998349649772 2307 74-75 0.3203882176304589 2307 76-77 0.3757847770208187 2307 78-79 0.42395365564673426 2307 80-81 0.32707887854388673 2307 82-83 0.4711252438362763 2307 84-85 0.4152943949970975 2307 86-87 0.3842293964685801 2307 88-89 0.5583460158915834 2307 90-91 0.44238785535868175 2307 92-93 0.46314533493795196 2307 94-95 0.34712981435995616 2307 96-97 0.39600021470154445 2307 98-99 0.30216835469147085 2307 100-101 0.19848864276381661 2201 1 0.3191554877562126 2201 2 0.28416752280429236 2201 3 0.2510505743057827 2201 4 0.3383665692769071 2201 5 0.372589696747589 2201 6 0.33929469169915905 2201 7 0.47306723860593536 2201 8 0.2994181150395505 2201 9 0.4449503961799195 2201 10-11 0.45917433820905984 2201 12-13 0.4109833110065537 2201 14-15 0.6751821038613528 2201 16-17 0.5323703380772642 2201 18-19 0.5064878571516331 2201 20-21 0.4684639294931259 2201 22-23 0.33151918234901956 2201 24-25 0.532685096199053 2201 26-27 0.5420119790221918 2201 28-29 0.4796956874399214 2201 30-31 0.5324293797067199 2201 32-33 0.5916164634159031 2201 34-35 0.416276607376858 2201 36-37 0.4459770013387825 2201 38-39 0.51117019109914 2201 40-41 0.49678159376755815 2201 42-43 0.42438666855776574 2201 44-45 0.5592774015740218 2201 46-47 0.4125979903211814 2201 48-49 0.4580776958732429 2201 50-51 0.4231087914767073 2201 52-53 0.4398521429625717 2201 54-55 0.6143459524330908 2201 56-57 0.589898067807411 2201 58-59 0.6638847289726613 2201 60-61 0.5853934944146175 2201 62-63 0.5610208305419491 2201 64-65 0.6133548205599766 2201 66-67 0.5646406218836333 2201 68-69 0.6083906523259301 2201 70-71 0.5844848196842065 2201 72-73 0.4222411256644918 2201 74-75 0.5651515894521779 2201 76-77 0.584873912128117 2201 78-79 0.5242971507278966 2201 80-81 0.5955610071577198 2201 82-83 0.5445239646904767 2201 84-85 0.5203356623964552 2201 86-87 0.6798374466505557 2201 88-89 0.7364142680809742 2201 90-91 0.6836967087380827 2201 92-93 0.5964621711279783 2201 94-95 0.6065911429247883 2201 96-97 0.5431185166342907 2201 98-99 0.5395680112697683 2201 100-101 0.49382108286742366 1101 1 0.14486721684282955 1101 2 0.08640538766006856 1101 3 0.08268752653290434 1101 4 0.15488950916491007 1101 5 0.19883024232218105 1101 6 0.14520795507009154 1101 7 0.35525138634145037 1101 8 0.0931063218640773 1101 9 0.18856686917176546 1101 10-11 0.18471851335741007 1101 12-13 0.2031473456902475 1101 14-15 0.402171185006317 1101 16-17 0.38224783155290964 1101 18-19 0.2829211447323132 1101 20-21 0.2634116748985633 1101 22-23 0.1974372999263565 1101 24-25 0.23084847395162456 1101 26-27 0.2653784982376024 1101 28-29 0.1697567944720575 1101 30-31 0.21060157902858379 1101 32-33 0.39271747421471304 1101 34-35 0.2222418511328712 1101 36-37 0.29327338817703463 1101 38-39 0.21731241150561686 1101 40-41 0.15482638864737197 1101 42-43 0.15978176882853035 1101 44-45 0.24378118131634352 1101 46-47 0.1289529481706566 1101 48-49 0.27412163662543065 1101 50-51 0.21630328603516347 1101 52-53 0.22141936666879758 1101 54-55 0.2628124769975031 1101 56-57 0.30459276269426283 1101 58-59 0.2917396902244036 1101 60-61 0.3314804213905802 1101 62-63 0.37650285589603527 1101 64-65 0.34729072980416476 1101 66-67 0.2538794384288323 1101 68-69 0.15286049137780822 1101 70-71 0.2898849620748578 1101 72-73 0.1340999266601841 1101 74-75 0.28861672945020445 1101 76-77 0.2812974002683646 1101 78-79 0.23150065422837685 1101 80-81 0.35398256181691323 1101 82-83 0.36641314142797654 1101 84-85 0.39269635102142075 1101 86-87 0.38839694648163814 1101 88-89 0.4984195402033542 1101 90-91 0.41685394865939784 1101 92-93 0.40322591200870406 1101 94-95 0.3663489562390936 1101 96-97 0.29742749227913023 1101 98-99 0.2541636572737467 1101 100-101 0.24111565816559377 2304 1 0.29063563616445265 2304 2 0.2909217895629048 2304 3 0.25677935326940826 2304 4 0.47881268560831103 2304 5 0.4393902796463749 2304 6 0.4094755829967127 2304 7 0.5955859337077314 2304 8 0.3447050269786516 2304 9 0.4859567294738909 2304 10-11 0.4462206502173629 2304 12-13 0.4463764490935844 2304 14-15 0.619267684118995 2304 16-17 0.5407839656957592 2304 18-19 0.5760339837692356 2304 20-21 0.6108307803761619 2304 22-23 0.4892078508132798 2304 24-25 0.5663880583399887 2304 26-27 0.5869800624651802 2304 28-29 0.6121056772689784 2304 30-31 0.6284534072102161 2304 32-33 0.6762344074516164 2304 34-35 0.6578575539545497 2304 36-37 0.6547911598379343 2304 38-39 0.5649442997722218 2304 40-41 0.46849469291928614 2304 42-43 0.4549688040280131 2304 44-45 0.5403457608305189 2304 46-47 0.5323659442407802 2304 48-49 0.5256452199473927 2304 50-51 0.46342832021682767 2304 52-53 0.5440304033433137 2304 54-55 0.697688734711349 2304 56-57 0.60924944177534 2304 58-59 0.7092313573738167 2304 60-61 0.664933538477797 2304 62-63 0.5218211050724051 2304 64-65 0.6144766462744613 2304 66-67 0.6550590872749424 2304 68-69 0.6277178004603812 2304 70-71 0.6368011377402567 2304 72-73 0.5460819771612222 2304 74-75 0.5909412070080364 2304 76-77 0.6300690094631207 2304 78-79 0.5204169507364611 2304 80-81 0.5003954962198875 2304 82-83 0.6114004436025837 2304 84-85 0.7090520346453033 2304 86-87 0.4689645707331387 2304 88-89 0.5111458321455835 2304 90-91 0.6847555777309111 2304 92-93 0.6454983026320598 2304 94-95 0.6270461188653194 2304 96-97 0.7473624303285646 2304 98-99 0.6190307022901429 2304 100-101 0.6583757309328213 2202 1 0.221548469028221 2202 2 0.17760580794481484 2202 3 0.21689693656643882 2202 4 0.30832753946158675 2202 5 0.2977699264362954 2202 6 0.25677789786711713 2202 7 0.36560853502258794 2202 8 0.23069749037114917 2202 9 0.27462900228755416 2202 10-11 0.2812455147573871 2202 12-13 0.31702954375672476 2202 14-15 0.4498137604251653 2202 16-17 0.36752768836375793 2202 18-19 0.3725451366064405 2202 20-21 0.39724589937771526 2202 22-23 0.334784661929973 2202 24-25 0.34567799150282497 2202 26-27 0.4313628071956188 2202 28-29 0.42270876635381427 2202 30-31 0.4214318405402011 2202 32-33 0.4195630944542472 2202 34-35 0.3634472657447603 2202 36-37 0.42841371033897957 2202 38-39 0.38176656921184104 2202 40-41 0.2889322059586945 2202 42-43 0.308731355453304 2202 44-45 0.4205656175425503 2202 46-47 0.34968840481222685 2202 48-49 0.3903122435481592 2202 50-51 0.4153908487101674 2202 52-53 0.41620487644495796 2202 54-55 0.5355647022376928 2202 56-57 0.570703033671272 2202 58-59 0.5569678905072948 2202 60-61 0.45298713195924023 2202 62-63 0.6045415694038354 2202 64-65 0.6238458957634663 2202 66-67 0.5464230221969189 2202 68-69 0.641211669063388 2202 70-71 0.6401025268249185 2202 72-73 0.5857458248992344 2202 74-75 0.5952164693284985 2202 76-77 0.5798567342170458 2202 78-79 0.5679231782310978 2202 80-81 0.5829266849322607 2202 82-83 0.5747743061651747 2202 84-85 0.5260903014228049 2202 86-87 0.4513977089128467 2202 88-89 0.643280149193938 2202 90-91 0.6124361267365543 2202 92-93 0.5066304604804621 2202 94-95 0.48473055624841876 2202 96-97 0.5203745964448458 2202 98-99 0.5228219766628328 2202 100-101 0.6657627006725058 1102 1 0.04297955545208154 1102 2 0.016707756867571533 1102 3 -0.010731565280423183 1102 4 0.02529607871051809 1102 5 0.14212818944888284 1102 6 0.12102415461328775 1102 7 0.20746948329433934 1102 8 0.07314434653999058 1102 9 0.12143349827140071 1102 10-11 0.11230146469258528 1102 12-13 0.12975162631084913 1102 14-15 0.18048863306189133 1102 16-17 0.06430387774985746 1102 18-19 0.13780039695235047 1102 20-21 0.21143300708340718 1102 22-23 0.1237628251302425 1102 24-25 0.19929905774135648 1102 26-27 0.23461347222552575 1102 28-29 0.17578720919758695 1102 30-31 0.14711651686312877 1102 32-33 0.16535807966936034 1102 34-35 0.1346746052332506 1102 36-37 0.1869233676029225 1102 38-39 0.16987231051814433 1102 40-41 0.0846951774990714 1102 42-43 0.07814545631308789 1102 44-45 0.21325253613623119 1102 46-47 0.17505986596756173 1102 48-49 0.22051228194705885 1102 50-51 0.10053272723056494 1102 52-53 0.17399811189054049 1102 54-55 0.30991456083970803 1102 56-57 0.2823327252855208 1102 58-59 0.3109953049254415 1102 60-61 0.29540239040490235 1102 62-63 0.31968672664410747 1102 64-65 0.33565838713886365 1102 66-67 0.3786423229473357 1102 68-69 0.3912267381854164 1102 70-71 0.39754240412943176 1102 72-73 0.4429843196245713 1102 74-75 0.5097382246527999 1102 76-77 0.468797140692196 1102 78-79 0.38137293883638534 1102 80-81 0.427004746195216 1102 82-83 0.4450448549198356 1102 84-85 0.40469215909161704 1102 86-87 0.36109037617591255 1102 88-89 0.43013586486813793 1102 90-91 0.40218299759504816 1102 92-93 0.5047779071326666 1102 94-95 0.3598682307391847 1102 96-97 0.37441774404802786 1102 98-99 0.48929934645375894 1102 100-101 0.34373481498854375 2305 1 0.1832336220814028 2305 2 0.144397787112176 2305 3 0.1557546216412291 2305 4 0.18557925821540522 2305 5 0.24015861263453075 2305 6 0.3904553774463366 2305 7 0.26762100757470364 2305 8 0.34356196581403964 2305 9 0.21582895083317766 2305 10-11 0.235724539882888 2305 12-13 0.23219545775742034 2305 14-15 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-1.1233388178100832 1213 94-95 -1.0509683226522633 1213 96-97 -1.0953440933619092 1213 98-99 -1.1680933023844595 1213 100-101 -1.0257236510730472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4278.0 3 596.0 4 573.0 5 475.0 6 998.0 7 5545.0 8 20938.0 9 55967.0 10 75447.0 11 56828.0 12 36422.0 13 25838.0 14 20174.0 15 17488.0 16 16729.0 17 17169.0 18 18339.0 19 19950.0 20 21577.0 21 24056.0 22 27207.0 23 29192.0 24 32242.0 25 36964.0 26 42932.0 27 51532.0 28 61416.0 29 74979.0 30 92218.0 31 116787.0 32 155838.0 33 216955.0 34 324470.0 35 528075.0 36 962169.0 37 1744101.0 38 919803.0 39 6991.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.28271870823996 26.316567048183654 20.829493796750935 16.571220446825446 2 30.562413487473805 26.462335250058818 28.94916004201842 14.026091220448963 3 34.87155796154003 22.74805562021724 25.948792045621182 16.431594372621547 4 29.27954774133022 22.030672329015584 30.656997807654356 18.032782121999837 5 31.83495591740616 20.30739523631744 30.451725217814644 17.405923628461753 6 28.88690475885213 20.739993890338237 33.28076699333014 17.092334357479487 7 32.861100813977714 21.45953363300128 26.999904121297323 18.67946143172368 8 28.427055332560524 29.72383878664272 24.1655118150337 17.683594065763053 9 30.188489356390686 27.40973525919529 24.11455043238942 18.2872249520246 10-11 26.636491639721932 29.792090100696466 27.647437049011526 15.923981210570076 12-13 27.610561727839656 27.397507485822377 26.96402490172922 18.02790588460875 14-15 25.532098145167076 27.60950589055563 28.269181474228176 18.589214490049113 16-17 25.82816936621605 27.850218906976647 28.348562418714675 17.973049308092634 18-19 25.135888684528158 28.459339851706844 28.738272741805133 17.66649872195986 20-21 24.08035279839134 29.2990212433469 28.59028881673016 18.030337141531604 22-23 25.557353946019816 28.636291424666894 27.60019644687393 18.20615818243936 24-25 23.00935180475206 30.63987750608601 28.17693314028084 18.17383754888108 26-27 22.59389765253195 30.934944655366024 27.868507566440297 18.60265012566173 28-29 23.029039096620345 30.760772219842586 27.745584515856713 18.464604167680353 30-31 24.203102773758033 28.738677844911937 28.425739199323807 18.632480182006226 32-33 24.25573587831217 28.90543712208462 28.1278290985366 18.710997901066612 34-35 22.219857275665404 28.818041059445488 28.284328323287205 20.677773341601903 36-37 21.145740849553807 28.581699766289688 28.39863908928853 21.87392029486798 38-39 20.766738363374476 29.926396361692976 29.312681897810545 19.994183377122003 40-41 21.476751782704888 28.710732253492143 30.623813009691876 19.1887029541111 42-43 22.298911280347664 28.658107447616228 29.802200379541077 19.24078089249503 44-45 21.254447619765592 29.554494845976574 30.10580673603731 19.085250798220514 46-47 22.458464720101144 29.876642728675883 28.15101210856686 19.513880442656113 48-49 20.721066168114767 29.783053593872793 29.439791437905093 20.05608880010734 50-51 20.429511247386177 28.50864193008312 30.396859381631913 20.664987440898788 52-53 23.14486849276053 28.614446489260608 28.12101192383425 20.119673094144613 54-55 23.382705427148526 28.537154074917513 28.7853321827096 19.29480831522436 56-57 23.854781027779897 28.26228238633402 28.245326672245536 19.63760991364055 58-59 22.74401545795951 28.889124331378042 28.153317106058832 20.21354310460362 60-61 21.071673585374903 28.382613534158523 28.32992224463011 22.21579063583646 62-63 22.3795718518943 28.66415515867704 28.153117880787903 20.803155108640752 64-65 20.825407220232478 29.454561100120973 29.802638607648912 19.917393071997633 66-67 20.486781336433047 29.110393403277623 28.771812822314104 21.63101243797523 68-69 20.20171602000803 28.92377970636778 31.01393218254434 19.86057209107985 70-71 20.084473976637 30.26613527879047 29.84906832638136 19.800322418191175 72-73 20.10938645661511 32.07031930286641 27.974473014950846 19.845821225567633 74-75 19.85560057767296 32.15102945468343 28.392545610126863 19.600824357516743 76-77 20.184734621899977 31.860289823138295 27.966516336552864 19.988459218408863 78-79 20.012286938507053 32.17553487830208 28.050112300941375 19.762065882249487 80-81 19.95607594054082 31.616476398945785 28.394722781777247 20.032724878736143 82-83 20.155621959445668 31.627831914318843 27.917877511777156 20.298668614458332 84-85 19.975603664276235 31.36502683622599 28.5330428225342 20.126326676963576 86-87 20.311047603873426 30.812431807040376 28.306028482248802 20.5704921068374 88-89 20.265522844359566 31.243641509934434 28.215823730890303 20.2750119148157 90-91 20.331775892805208 30.624193763622614 28.48626874659969 20.557761596972487 92-93 20.41722994037153 30.785329128316587 28.05846289270006 20.738978038611826 94-95 20.1482111531215 30.68222515685753 28.608249252439244 20.561314437581725 96-97 20.43916961353926 30.406850160605163 28.240482935022666 20.91349729083291 98-99 20.362061784343247 30.740350190148902 28.200779996730553 20.696808028777298 100-101 20.513689959606793 30.317169119701187 28.3680550297026 20.80108589098942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3013.0 1 1936.5 2 731.0 3 514.0 4 473.5 5 554.5 6 683.0 7 897.0 8 1199.0 9 1558.5 10 1937.5 11 2201.0 12 2358.5 13 2396.0 14 2294.0 15 2161.5 16 2069.0 17 2197.5 18 2542.0 19 3160.5 20 4194.5 21 5742.5 22 8069.5 23 11528.5 24 16062.5 25 21942.5 26 29484.0 27 38690.5 28 49422.0 29 61592.0 30 75312.0 31 91270.5 32 110267.0 33 131703.5 34 155245.0 35 181354.0 36 210285.5 37 239994.0 38 266560.5 39 286835.0 40 300137.5 41 304381.5 42 299047.0 43 288586.5 44 277236.0 45 266933.0 46 257334.0 47 244788.0 48 227193.0 49 208524.5 50 190080.5 51 171074.0 52 150122.5 53 127444.5 54 106139.5 55 87694.0 56 71895.0 57 58183.5 58 46786.5 59 37072.5 60 28554.0 61 21693.0 62 16246.5 63 12013.0 64 8840.0 65 6398.5 66 4771.0 67 3533.0 68 2529.5 69 1859.5 70 1394.5 71 1067.0 72 844.0 73 650.0 74 531.5 75 455.0 76 375.0 77 296.0 78 233.0 79 220.0 80 192.0 81 163.5 82 140.0 83 105.5 84 89.5 85 82.0 86 75.5 87 63.5 88 51.0 89 39.5 90 37.0 91 32.5 92 23.5 93 24.5 94 23.0 95 15.0 96 7.5 97 5.0 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2577577176375319 2 0.24549491084990632 3 0.20964453551250858 4 0.21228811694794944 5 0.2273480034479124 6 0.23036680289354486 7 0.20662573606687612 8 0.20222545212917462 9 0.18383976962978604 10-11 0.1718072102575053 12-13 0.19948806619118586 14-15 0.19644368369940401 16-17 0.19735614567873358 18-19 0.18988589620310073 20-21 0.190858051956779 22-23 0.15789856083426654 24-25 0.46969619962143916 26-27 0.32738624157422375 28-29 0.27616898318306987 30-31 0.28013435533623116 32-33 0.3216812222829014 34-35 0.31781818231433784 36-37 0.3104332096592031 38-39 0.3353255135625961 40-41 0.34043359511043175 42-43 0.37509862264290605 44-45 0.3428895675407768 46-47 0.3678415652185184 48-49 0.34455246554048957 50-51 0.3663236378136524 52-53 0.3554678985642453 54-55 0.3320849943836686 56-57 0.36787567594671766 58-59 0.35528028955914953 60-61 0.32129747659066005 62-63 0.29847739942537066 64-65 0.30468555195763175 66-67 0.3140915852585713 68-69 0.32070906652922315 70-71 0.2973432177127461 72-73 0.3041994740807926 74-75 0.30249393767083077 76-77 0.3052910173831682 78-79 0.3074485209417699 80-81 0.3254248747027676 82-83 0.342122076156294 84-85 0.3369713561982093 86-87 0.341184031130815 88-89 0.3358712852137839 90-91 0.31037351588485446 92-93 0.29377864661592584 94-95 0.3026730189938768 96-97 0.29145058941632795 98-99 0.3109789813103909 100-101 0.33390139066027796 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5863258.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.71350465078348 #Duplication Level Percentage of deduplicated Percentage of total 1 96.87598167194025 88.84835795618709 2 2.1948967695867023 4.026033501709593 3 0.398933587369076 1.0976279226158248 4 0.10887463603067088 0.3994109775180517 5 0.1252916223405849 0.574546689411872 6 0.06359725845929008 0.3499636475689908 7 0.04480832913813789 0.2876670231963112 8 0.022175815068743887 0.16270573747537254 9 0.015173330605471283 0.12524393943474893 >10 0.1326032877373335 2.186661456286405 >50 0.011087085758721969 0.7334236570710437 >100 0.006486995203314407 1.030039251596525 >500 7.09963533130458E-5 0.0353408567904552 >1k 0.0 0.0 >5k 1.861440843396591E-5 0.1429773831377097 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 8375 0.14283867433430356 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013985398561687035 0.0 1.7055364099618336E-5 2 0.0015349827689656501 0.0 1.7055364099618336E-5 3 0.0016884810458622152 0.0 3.411072819923667E-5 4 0.00184197932275878 0.0 3.411072819923667E-5 5 0.0023024741534484754 0.0 3.411072819923667E-5 6 0.0027629689841381705 0.0 5.1166092298855005E-5 7 0.0033940174558240487 0.0 5.1166092298855005E-5 8 0.0040591766557091635 0.0 5.1166092298855005E-5 9 0.004639059035096187 0.0 5.1166092298855005E-5 10-11 0.006608953588602105 0.0 8.527682049809167E-5 12-13 0.008271851588314894 0.0 9.380450254790085E-5 14-15 0.009704502172682834 0.0 1.0233218459771001E-4 16-17 0.011324761762146574 0.0 1.1938754869732835E-4 18-19 0.013379933136150584 0.0 1.4497059484675585E-4 20-21 0.015895599340844287 0.0 1.876090050958017E-4 22-23 0.018624457596783224 0.0 1.876090050958017E-4 24-25 0.021890559821860132 0.0 2.3024741534484754E-4 26-27 0.025003163770040478 0.0 2.643581435440842E-4 28-29 0.028729760825807082 0.0 2.728858255938934E-4 30-31 0.044727692351249085 0.0 2.899411896935117E-4 32-33 0.049827246217034965 0.0 2.899411896935117E-4 34-35 0.05467949730337638 0.0 3.155242358429392E-4 36-37 0.06009457540500521 0.0 3.58162646091985E-4 38-39 0.06570579019377963 0.0 3.752180101916034E-4 40-41 0.07231474378238174 0.0 3.8374569224141253E-4 42-43 0.07946094134012183 0.0 4.008010563410309E-4 44-45 0.08743432405669341 0.0 4.0932873839084004E-4 46-47 0.09569764796295849 0.0 4.178564204406492E-4 48-49 0.10475404629985582 0.0 4.60494830689695E-4 50-51 0.11480818343658082 0.0 4.775501947893134E-4 52-53 0.12575772718853578 0.0 4.775501947893134E-4 54-55 0.1373980131865253 0.0 4.946055588889317E-4 56-57 0.1496608199741509 0.0 5.287162870881684E-4 58-59 0.1637059123101866 0.0 5.542993332375959E-4 60-61 0.17804947351796563 0.0 5.62827015287405E-4 62-63 0.19375746385371412 0.0 5.713546973372143E-4 64-65 0.21007944729704883 0.0 5.969377434866417E-4 66-67 0.22788524741705038 0.0 5.969377434866417E-4 68-69 0.24843696115709046 0.0 6.139931075862601E-4 70-71 0.269773221645713 0.0 6.395761537356876E-4 72-73 0.2939406725748722 0.0 6.566315178353059E-4 74-75 0.31984777064219244 0.0 6.822145639847334E-4 76-77 0.3503854000625591 0.0 6.907422460345426E-4 78-79 0.38544270096932454 0.0 7.077976101341609E-4 80-81 0.423407941455075 1.7055364099618336E-5 7.248529742337792E-4 82-83 0.46615721157076834 1.7055364099618336E-5 7.333806562835884E-4 84-85 0.514389781244489 1.7055364099618336E-5 7.589637024330159E-4 86-87 0.5668350258508155 2.5583046149427503E-5 7.930744306322526E-4 88-89 0.6256078105381002 3.411072819923667E-5 8.016021126820617E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4140 0.0 81.9539 2 GATCGGG 4460 0.0 77.145035 1 GAGCGGC 2620 0.0 76.58167 9 GAGAGCG 3900 0.0 67.50565 7 TCGGGAG 4080 0.0 66.51933 3 AGAGCGG 3155 0.0 65.85297 8 AAGCGGC 1155 0.0 64.4768 9 CGGGAGA 4315 0.0 63.4464 4 GTTCGGA 680 0.0 62.107643 2 GATCCGG 840 0.0 61.01678 1 TCGGGAA 1560 0.0 60.22164 3 TTCGGAA 990 0.0 57.99121 3 AAGCGTC 635 0.0 56.770885 9 GGATCGG 1605 0.0 55.88453 1 TCGGAGA 1320 0.0 55.714695 4 GAGCGTC 8885 0.0 54.93432 9 GGTTCGG 900 0.0 54.312466 1 AGAGCGT 9375 0.0 52.874424 8 AAAGCGG 1545 0.0 51.58001 8 GATTCGG 1365 0.0 50.412727 1 >>END_MODULE