FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_35008-2.342000000358b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_35008-2.342000000358b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5863258
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT83750.14283867433430356Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA41400.081.95392
GATCGGG44600.077.1450351
GAGCGGC26200.076.581679
GAGAGCG39000.067.505657
TCGGGAG40800.066.519333
AGAGCGG31550.065.852978
AAGCGGC11550.064.47689
CGGGAGA43150.063.44644
GTTCGGA6800.062.1076432
GATCCGG8400.061.016781
TCGGGAA15600.060.221643
TTCGGAA9900.057.991213
AAGCGTC6350.056.7708859
GGATCGG16050.055.884531
TCGGAGA13200.055.7146954
GAGCGTC88850.054.934329
GGTTCGG9000.054.3124661
AGAGCGT93750.052.8744248
AAAGCGG15450.051.580018
GATTCGG13650.050.4127271
Produced by FastQC (version 0.11.2)