FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422980
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC55750.23008856862210997No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46640.19249023929211137No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC42320.17466095469215592No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37410.15439665205655845No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG36380.15014568836721723No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34050.14052943070103757No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29830.1231128610223774No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC29630.12228743117978687No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT28330.11692213720294843No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG27010.11147430024185095No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26830.11073141338351948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC5650.067.998369
CGGGAGA7500.063.900124
AGAGCGG6100.062.9860048
CGGAAAG3300.061.8244325
GAGAGCG7800.057.171027
AACCGGA1450.055.6397822
TCGGGAG10050.053.8256573
TTGTGTT305050.051.2198451
ACCGGAA1400.050.840973
TCGGGAA1800.050.0877653
GATCCGG2200.049.619751
GAACCGG1650.048.9006231
GATCCGA902.2737368E-1047.462371
GGATCGG4250.044.6704671
ATCCGGA2550.042.804772
TCGGAGA2900.042.5419044
GTTGACG459.6648035E-442.1887781
ATTCGGA1350.042.184412
GGACCGG1600.041.5295751
ATCGGGA14450.040.3963172
Produced by FastQC (version 0.11.2)