##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422980 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811598527433162 33.0 33.0 33.0 27.0 33.0 2 31.755373548275266 33.0 33.0 33.0 27.0 33.0 3 31.708138738247943 33.0 33.0 33.0 27.0 33.0 4 35.393893882739434 37.0 37.0 37.0 33.0 37.0 5 35.360991836498854 37.0 37.0 37.0 33.0 37.0 6 35.420798355743756 37.0 37.0 37.0 33.0 37.0 7 35.35583207455282 37.0 37.0 37.0 33.0 37.0 8 35.362740922335306 37.0 37.0 37.0 33.0 37.0 9 35.215411187876086 37.0 37.0 37.0 33.0 37.0 10-11 35.14724554061527 37.0 37.0 37.0 33.0 37.0 12-13 35.153675432731596 37.0 37.0 37.0 33.0 37.0 14-15 36.83335025464511 40.0 37.0 40.0 33.0 40.0 16-17 36.78607933206217 40.0 37.0 40.0 33.0 40.0 18-19 36.81756019447127 40.0 37.0 40.0 33.0 40.0 20-21 36.808955501077186 40.0 37.0 40.0 33.0 40.0 22-23 36.89221351393738 40.0 37.0 40.0 33.0 40.0 24-25 36.64375521052588 40.0 37.0 40.0 33.0 40.0 26-27 36.540061824695215 40.0 37.0 40.0 33.0 40.0 28-29 36.45040157161842 40.0 37.0 40.0 33.0 40.0 30-31 36.48757088378773 40.0 37.0 40.0 33.0 40.0 32-33 36.36619967973322 40.0 37.0 40.0 33.0 40.0 34-35 36.31151392087429 40.0 37.0 40.0 33.0 40.0 36-37 36.20380791422133 40.0 37.0 40.0 33.0 40.0 38-39 36.149080883870276 40.0 37.0 40.0 33.0 40.0 40-41 36.10166138391567 38.5 37.0 40.0 33.0 40.0 42-43 35.93014201520442 37.0 37.0 40.0 27.0 40.0 44-45 35.81612972455406 37.0 37.0 40.0 27.0 40.0 46-47 35.75977886734517 37.0 37.0 40.0 27.0 40.0 48-49 35.639578535522375 37.0 37.0 40.0 27.0 40.0 50-51 34.49306989739907 37.0 35.0 38.5 27.0 38.5 52-53 34.92961786725437 37.0 35.0 38.5 27.0 40.0 54-55 35.702629819478496 37.0 37.0 40.0 27.0 40.0 56-57 35.765863729787284 37.0 37.0 40.0 27.0 40.0 58-59 35.545574045184026 37.0 37.0 40.0 27.0 40.0 60-61 35.263468951456474 37.0 37.0 40.0 27.0 40.0 62-63 35.060980486838524 37.0 37.0 40.0 27.0 40.0 64-65 34.80056335586757 37.0 33.0 40.0 27.0 40.0 66-67 34.55766267158623 37.0 33.0 37.0 27.0 40.0 68-69 34.34427523132672 37.0 33.0 37.0 27.0 40.0 70-71 34.02048056525436 37.0 33.0 37.0 27.0 40.0 72-73 33.80064094627277 37.0 33.0 37.0 27.0 38.5 74-75 33.57368447118837 37.0 33.0 37.0 27.0 37.0 76-77 33.32588754343824 37.0 33.0 37.0 27.0 37.0 78-79 33.07367002616613 37.0 33.0 37.0 27.0 37.0 80-81 32.860105943920296 37.0 33.0 37.0 27.0 37.0 82-83 32.65920147917028 37.0 33.0 37.0 22.0 37.0 84-85 32.509030615192856 37.0 33.0 37.0 22.0 37.0 86-87 32.35676295305781 35.0 33.0 37.0 22.0 37.0 88-89 32.15136753089171 33.0 33.0 37.0 22.0 37.0 90-91 32.08812206456513 33.0 33.0 37.0 22.0 37.0 92-93 31.931606946817553 33.0 33.0 37.0 22.0 37.0 94-95 31.797410832941253 33.0 33.0 37.0 22.0 37.0 96-97 31.654908831273886 33.0 33.0 37.0 22.0 37.0 98-99 31.514757860155676 33.0 33.0 37.0 18.5 37.0 100-101 30.29930478171508 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2624838712400468 2306 2 0.2836141500664233 2306 3 0.2629570396393426 2306 4 0.3049496073272735 2306 5 0.3151500178560056 2306 6 0.2811607047796869 2306 7 0.3154154116662866 2306 8 0.34524564656576473 2306 9 0.37469397554917805 2306 10-11 0.3793959208772364 2306 12-13 0.35797901104439944 2306 14-15 0.4330323296623959 2306 16-17 0.44528869684771166 2306 18-19 0.45523573696284103 2306 20-21 0.4260906798898958 2306 22-23 0.43133700088337434 2306 24-25 0.4634873501437369 2306 26-27 0.42058612510216165 2306 28-29 0.4924062407187151 2306 30-31 0.3904806302795336 2306 32-33 0.40966169641259853 2306 34-35 0.3425831925275773 2306 36-37 0.3731139330433493 2306 38-39 0.41031811672662855 2306 40-41 0.334739243048908 2306 42-43 0.21616046314829873 2306 44-45 0.30672579141587875 2306 46-47 0.2682930556806724 2306 48-49 0.3197670116514857 2306 50-51 0.39481183301793266 2306 52-53 0.3429763151887215 2306 54-55 0.3273146138495733 2306 56-57 0.27079929974031103 2306 58-59 0.35969932802790794 2306 60-61 0.4735617813843973 2306 62-63 0.41574377093727577 2306 64-65 0.37772978465606144 2306 66-67 0.35944478210402764 2306 68-69 0.33374042525977643 2306 70-71 0.28586373003847854 2306 72-73 0.39763943304416216 2306 74-75 0.4143282100724477 2306 76-77 0.18400671069059626 2306 78-79 0.11910892136340578 2306 80-81 0.19891981814857473 2306 82-83 0.2869566534104777 2306 84-85 0.2650236853065948 2306 86-87 0.39933887531164913 2306 88-89 0.2363702945032813 2306 90-91 0.025305225789509223 2306 92-93 0.16235916164866282 2306 94-95 0.08828592041972172 2306 96-97 0.196557142541959 2306 98-99 0.3185289793082582 2306 100-101 0.08367667252204214 2307 1 0.27169369293424595 2307 2 0.36500955656000755 2307 3 0.270126459897579 2307 4 0.34661668719795813 2307 5 0.3898916827045724 2307 6 0.41134054769604944 2307 7 0.40704084057205137 2307 8 0.3697116216941865 2307 9 0.43900579252293426 2307 10-11 0.3468861031497923 2307 12-13 0.2984300314930408 2307 14-15 0.449354177576879 2307 16-17 0.4919479423702313 2307 18-19 0.40915662988019363 2307 20-21 0.3248647035714427 2307 22-23 0.3591062942053398 2307 24-25 0.5017860321881997 2307 26-27 0.39891510326515345 2307 28-29 0.4408001993454249 2307 30-31 0.3952614420766736 2307 32-33 0.4838768980231052 2307 34-35 0.3672548192952618 2307 36-37 0.4227215958292021 2307 38-39 0.05625875914317646 2307 40-41 0.30498619856170706 2307 42-43 0.32760939609024575 2307 44-45 0.20360643165071224 2307 46-47 0.2034396522018227 2307 48-49 0.06255271743700064 2307 50-51 0.12553227074974416 2307 52-53 0.16999725471549 2307 54-55 0.18240334528018565 2307 56-57 0.24683449976411254 2307 58-59 0.21809286518651305 2307 60-61 0.23439487505940093 2307 62-63 0.2363230664409528 2307 64-65 0.10626664576000877 2307 66-67 0.23952818320281466 2307 68-69 0.16908473990306305 2307 70-71 0.2920051400228161 2307 72-73 0.2721002018019689 2307 74-75 0.19188280258717327 2307 76-77 0.21390231056366105 2307 78-79 0.249294590456401 2307 80-81 0.009622256713399224 2307 82-83 0.07930142918944938 2307 84-85 0.24494568891635282 2307 86-87 0.33873349837042355 2307 88-89 0.472642582727957 2307 90-91 0.32543823616258294 2307 92-93 0.3101079022028195 2307 94-95 0.19869593450171763 2307 96-97 0.16469068346899363 2307 98-99 0.24052230027852417 2307 100-101 0.1508526131396657 2201 1 0.3140882217657541 2201 2 0.31647639111451653 2201 3 0.281318824419305 2201 4 0.4389186783021799 2201 5 0.29584410435374764 2201 6 0.35084323694020725 2201 7 0.5023691809513551 2201 8 0.4397012901346926 2201 9 0.49335212197053835 2201 10-11 0.4884856811511682 2201 12-13 0.5073687296405751 2201 14-15 0.6948392243537711 2201 16-17 0.46063206443457716 2201 18-19 0.3908857701956876 2201 20-21 0.5067649603410871 2201 22-23 0.4145301730222215 2201 24-25 0.5353630070544071 2201 26-27 0.6557204720676424 2201 28-29 0.5185171715907515 2201 30-31 0.430225533260284 2201 32-33 0.5791854156722138 2201 34-35 0.5186070618514123 2201 36-37 0.46489831202657683 2201 38-39 0.6106241991270736 2201 40-41 0.5028292718588077 2201 42-43 0.6030481312718692 2201 44-45 0.606267093098424 2201 46-47 0.5544922510448487 2201 48-49 0.46162605821513836 2201 50-51 0.5716857827202944 2201 52-53 0.5519968150544514 2201 54-55 0.732263209260644 2201 56-57 0.6834935174683068 2201 58-59 0.6137252411413314 2201 60-61 0.6789084418783844 2201 62-63 0.6573476475759819 2201 64-65 0.6292980468638163 2201 66-67 0.6287635148572761 2201 68-69 0.6620079065260427 2201 70-71 0.6669250182091702 2201 72-73 0.44788603655019443 2201 74-75 0.45028906457577733 2201 76-77 0.5092452034383044 2201 78-79 0.530818547709579 2201 80-81 0.6315240945534981 2201 82-83 0.4618334731669478 2201 84-85 0.5321689778467658 2201 86-87 0.6523260038115808 2201 88-89 0.7504188569852843 2201 90-91 0.6961141509044282 2201 92-93 0.6072467106929302 2201 94-95 0.4823245434256016 2201 96-97 0.5040287609632834 2201 98-99 0.615545921376178 2201 100-101 0.6938996302817024 1101 1 0.3438254315548015 1101 2 0.3799099112913389 1101 3 0.3351772294533255 1101 4 0.4824266145763687 1101 5 0.5049062143062528 1101 6 0.5458093065734317 1101 7 0.4455782658198899 1101 8 0.5283555573909382 1101 9 0.5270316981075851 1101 10-11 0.5837170667515963 1101 12-13 0.5674321948442653 1101 14-15 0.7661201040666441 1101 16-17 0.6739348566447063 1101 18-19 0.6253993367643176 1101 20-21 0.5439008286233431 1101 22-23 0.5654495750695148 1101 24-25 0.7124949394408944 1101 26-27 0.6887943798900409 1101 28-29 0.5494275639174546 1101 30-31 0.6485158015571812 1101 32-33 0.5433542611383331 1101 34-35 0.5140014613662558 1101 36-37 0.5923218219977429 1101 38-39 0.5587287217033605 1101 40-41 0.442936942259216 1101 42-43 0.5660007313951212 1101 44-45 0.6472564130764908 1101 46-47 0.6232989160068456 1101 48-49 0.6352922157143368 1101 50-51 0.5448584360093847 1101 52-53 0.6754468329712608 1101 54-55 0.6674413040818266 1101 56-57 0.7381731279663484 1101 58-59 0.7629188956919535 1101 60-61 0.8637518516850093 1101 62-63 0.8161544597993924 1101 64-65 0.7851291746229023 1101 66-67 0.790599114900651 1101 68-69 0.6234276492826822 1101 70-71 0.7313077050872394 1101 72-73 0.5632717074798563 1101 74-75 0.7887883726702896 1101 76-77 0.7320647640370481 1101 78-79 0.5828993290696758 1101 80-81 0.6526563294952368 1101 82-83 0.681984813268059 1101 84-85 0.8303657396130646 1101 86-87 0.8082631151586028 1101 88-89 0.7950397324945087 1101 90-91 0.7054776335563631 1101 92-93 0.8898662278272447 1101 94-95 0.8124972230432412 1101 96-97 0.691412987838671 1101 98-99 0.6423308746519218 1101 100-101 0.5929595223881776 2304 1 0.2717504425972983 2304 2 0.1831534878929162 2304 3 0.14531799001036916 2304 4 0.24293948637508578 2304 5 0.1837875470728747 2304 6 0.182344130018528 2304 7 0.20591737458638448 2304 8 0.2630719111174713 2304 9 0.29694985863629597 2304 10-11 0.2966032415480342 2304 12-13 0.22779088841295447 2304 14-15 0.3930908768745951 2304 16-17 0.34531479347330674 2304 18-19 0.23286468833234863 2304 20-21 0.29615558106311113 2304 22-23 0.3928847577082095 2304 24-25 0.41196239950040336 2304 26-27 0.45699261975802585 2304 28-29 0.3012722856049095 2304 30-31 0.4157387599457252 2304 32-33 0.38760153801743513 2304 34-35 0.41247676368059416 2304 36-37 0.2990210744416686 2304 38-39 0.2453437594978638 2304 40-41 0.2719955799903815 2304 42-43 0.25707089499072566 2304 44-45 0.17645881158971122 2304 46-47 0.1192342361558616 2304 48-49 0.24936285434556282 2304 50-51 0.3065774399346708 2304 52-53 0.41281088527518506 2304 54-55 0.398801781117605 2304 56-57 0.20549192687627027 2304 58-59 0.4125274079970396 2304 60-61 0.3057979218069349 2304 62-63 0.2905571233324906 2304 64-65 0.5123849688074671 2304 66-67 0.5009525997453395 2304 68-69 0.4560819733569872 2304 70-71 0.34505144420101175 2304 72-73 0.4602616900618841 2304 74-75 0.5424609397794811 2304 76-77 0.4719523389384932 2304 78-79 0.5347334249602014 2304 80-81 0.4492516991479647 2304 82-83 0.4807507623809073 2304 84-85 0.550162224349414 2304 86-87 0.2904421952562828 2304 88-89 0.51138220818018 2304 90-91 0.700013057046462 2304 92-93 0.627281325626246 2304 94-95 0.4638560749571248 2304 96-97 0.5493665010188273 2304 98-99 0.4502641779829979 2304 100-101 0.45665304940270346 2202 1 0.21916999653859293 2202 2 0.15836981093532643 2202 3 0.206536520794117 2202 4 0.2279624540067502 2202 5 0.1992969299735634 2202 6 0.2507775832345871 2202 7 0.23509694941812853 2202 8 0.17308686678460106 2202 9 0.27854311196868053 2202 10-11 0.2519044914144999 2202 12-13 0.1424142073840997 2202 14-15 0.31943540321913133 2202 16-17 0.3704373468567326 2202 18-19 0.34723844944523563 2202 20-21 0.34513004960279403 2202 22-23 0.25047980334672815 2202 24-25 0.3763186529870879 2202 26-27 0.41521912689758267 2202 28-29 0.18083880864968904 2202 30-31 0.22301659113659866 2202 32-33 0.2332504477967774 2202 34-35 0.1978356403438184 2202 36-37 0.27351394676063023 2202 38-39 0.2327842632427135 2202 40-41 0.15137189306234689 2202 42-43 0.09317814848927952 2202 44-45 0.20354548324159794 2202 46-47 0.11906835881606526 2202 48-49 0.14573195071465506 2202 50-51 0.23308903057669994 2202 52-53 0.24331095055785568 2202 54-55 0.23316094265067733 2202 56-57 0.2557097336158094 2202 58-59 0.25176199831859236 2202 60-61 0.17901362763134188 2202 62-63 0.3210951712618382 2202 64-65 0.29550583671093733 2202 66-67 0.20950136590280266 2202 68-69 0.30193800725650277 2202 70-71 0.2224354487589224 2202 72-73 0.1752728474643419 2202 74-75 0.40942582957045914 2202 76-77 0.38124621779759593 2202 78-79 0.3289864724741207 2202 80-81 0.15570251755529796 2202 82-83 0.19775304133970906 2202 84-85 0.29491409084041464 2202 86-87 0.19872181875329176 2202 88-89 0.386616929067074 2202 90-91 0.2254857629539444 2202 92-93 0.24163787585235852 2202 94-95 0.17841589169086447 2202 96-97 0.19875046996800805 2202 98-99 0.358369615436267 2202 100-101 0.6163338764748367 1102 1 0.11168293383527228 1102 2 0.17162760169340174 1102 3 0.12491717143722525 1102 4 0.22373124419033985 1102 5 0.3344720435798294 1102 6 0.3252921694417594 1102 7 0.31169560712701383 1102 8 0.21977809510824642 1102 9 0.2695641178040802 1102 10-11 0.2561503212648262 1102 12-13 0.2448315583256857 1102 14-15 0.4076675945844741 1102 16-17 0.21059364935268832 1102 18-19 0.2568250063527202 1102 20-21 0.3429671445848612 1102 22-23 0.2731431492590346 1102 24-25 0.3525358711443616 1102 26-27 0.34345970041570695 1102 28-29 0.2195902118862776 1102 30-31 0.24037945808511552 1102 32-33 0.17574928106073173 1102 34-35 0.255963461867033 1102 36-37 0.2560071728115787 1102 38-39 0.1912338401790663 1102 40-41 0.2187418693606915 1102 42-43 0.11200703217229346 1102 44-45 0.33651985174213195 1102 46-47 0.2025716858771176 1102 48-49 0.30392616439190334 1102 50-51 0.16938352277992408 1102 52-53 0.1707872689695833 1102 54-55 0.3500690405441347 1102 56-57 0.3372539761317839 1102 58-59 0.42831610792412533 1102 60-61 0.4491975877914598 1102 62-63 0.37351057286557676 1102 64-65 0.3566161451535237 1102 66-67 0.3928838913502588 1102 68-69 0.4736571856449956 1102 70-71 0.3581609636421206 1102 72-73 0.39130219531909916 1102 74-75 0.48139079652918326 1102 76-77 0.35408133477506 1102 78-79 0.27793962071208966 1102 80-81 0.35580204845287966 1102 82-83 0.28244259781948244 1102 84-85 0.3840177508942375 1102 86-87 0.2148723695559127 1102 88-89 0.42399834912531276 1102 90-91 0.41775468901785473 1102 92-93 0.48773518679812256 1102 94-95 0.3476422109625865 1102 96-97 0.4896882849437425 1102 98-99 0.4074731493564663 1102 100-101 0.3285193020783552 2305 1 0.154885850732704 2305 2 0.07794600768543702 2305 3 0.1673036119959903 2305 4 0.11697943541503264 2305 5 0.216424729710063 2305 6 0.3014473241217175 2305 7 0.21313823180724256 2305 8 0.30727293031849 2305 9 0.17074410743054358 2305 10-11 0.21435508429987493 2305 12-13 0.23171299233505493 2305 14-15 0.25356839985212076 2305 16-17 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-0.9166539039774655 1213 98-99 -1.016589482755716 1213 100-101 -0.7315919290552415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1849.0 3 246.0 4 200.0 5 190.0 6 341.0 7 2301.0 8 9277.0 9 14305.0 10 15004.0 11 13365.0 12 9984.0 13 7491.0 14 5932.0 15 5458.0 16 5190.0 17 5415.0 18 6006.0 19 6750.0 20 7633.0 21 8759.0 22 10237.0 23 10776.0 24 12328.0 25 14266.0 26 16868.0 27 20002.0 28 23741.0 29 29579.0 30 37444.0 31 48800.0 32 66938.0 33 96685.0 34 145583.0 35 247685.0 36 486221.0 37 823295.0 38 206689.0 39 147.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.001166090035554 24.840531601329623 26.367538503109504 14.790763805525314 2 29.37949741491735 22.794363460634585 35.052405020554886 12.773734103893181 3 38.30014889569029 19.844486723467618 26.0819339895773 15.773430391264787 4 28.091776769672123 18.64144803237409 36.57835264065225 16.688422557301543 5 31.75186180587414 17.66840640668881 34.23228404272174 16.347447744715318 6 24.319771581090244 18.488131243244048 41.1596264116397 16.03247076402601 7 32.15743171340288 18.691438424293207 31.96407899378831 17.187050868515602 8 27.543727669866175 27.688683556543342 27.887527466142153 16.880061307448337 9 30.655085681017614 25.73233980318775 25.436033950713 18.17654056508164 10-11 24.793327268190726 28.564630461444896 28.829809907185126 17.812232363179252 12-13 25.61544879608693 25.485881716794818 28.86192504091619 20.036744446202057 14-15 24.139307848452095 26.077316467934992 30.09290456888835 19.690471114724563 16-17 23.09828127135591 27.529371772123973 31.11951120210099 18.252835754419127 18-19 22.099800602245725 27.878799908364698 31.286797817624247 18.734601671765326 20-21 22.04148184047806 28.495618549347196 30.653799855516127 18.80909975465861 22-23 22.792384646022374 27.782491448734888 30.572594241669325 18.85252966357342 24-25 22.42722504578821 28.074009172984272 30.595398082721058 18.903367698506457 26-27 22.023668790958805 28.547140693948315 30.395651653631205 19.033538861461675 28-29 21.441716083899585 28.555955924180715 30.181639241542303 19.820688750377393 30-31 21.52186774866373 28.69534770095973 29.972627935481643 19.810156614894897 32-33 20.707091200736688 28.52079843188825 31.50725798237282 19.26485238500224 34-35 20.490334964443292 29.281083989431217 31.184487252023775 19.044093794101713 36-37 20.961697735639007 30.05861748679466 30.62299477251335 18.356690005052982 38-39 21.876026209679925 29.14071076550635 29.79529019190975 19.187972832903974 40-41 21.433441911730753 28.548222412676733 29.500062430335305 20.51827324525721 42-43 21.832548208436265 28.68734933245396 30.373520873992945 19.10658158511683 44-45 20.997957030973367 29.297865546712675 30.852269691790397 18.851907730523564 46-47 21.216406827686054 29.161845819896065 30.665452911045797 18.956294441372084 48-49 21.163270871801902 29.75048036314398 29.81123628358478 19.275012481469343 50-51 20.78823270144811 29.470130229498526 29.788931113988713 19.95270595506465 52-53 21.3362962073211 28.98550214397621 29.79912584172164 19.879075806981046 54-55 21.961713265031257 29.106432352848007 29.35499239205444 19.576861990066295 56-57 22.218602131940898 28.979379638959855 28.793381103335857 20.008637125763386 58-59 21.746991095681047 28.68062165383911 29.788649789134396 19.783737461345446 60-61 20.564382814080194 29.126040333287577 29.851895419397085 20.45768143323514 62-63 21.274259498629267 29.195205071869072 29.502241089715 20.028294339786665 64-65 20.983285479880333 29.16718846284332 29.621601347445047 20.227924709831303 66-67 21.088704209928157 28.832185616501455 29.38555315535011 20.693557018220286 68-69 21.195949324111 28.736508357132728 30.029056136206723 20.03848618254955 70-71 20.685497065783455 30.259551856453655 29.54595552508847 19.50899555267443 72-73 20.337771613978255 31.273288036306617 28.842421200250463 19.546519149464665 74-75 21.05060636186812 30.55563317616712 28.6176224454798 19.77613801648496 76-77 21.473792589099734 29.672642562573238 29.170367576695515 19.683197271631514 78-79 21.41703117648264 29.53379244561643 29.772815388879586 19.276360989021338 80-81 20.40540294337104 30.40182538361461 29.47468255368203 19.718089119332323 82-83 19.648829639200873 30.133477454196072 29.760026339330086 20.457666567272966 84-85 19.730404742815875 29.509522239817652 30.267193370129135 20.492879647237334 86-87 19.657916484361134 29.68850654136056 31.073766287657524 19.57981068662078 88-89 19.994823577156655 30.23088916450465 30.422851629227328 19.351435629111375 90-91 20.364371111963045 29.59128880998848 29.897943299348935 20.14639677869954 92-93 19.72277639209438 30.56271164270024 29.71451610466287 19.999995860542505 94-95 19.304357653651362 31.07060622644962 29.80498152399893 19.820054595900093 96-97 19.902092289390076 31.524167615781877 29.217212269074217 19.35652782575383 98-99 20.436567902604388 30.445796241263302 28.798875210835867 20.318760645296447 100-101 20.29805973576429 30.210781077014463 28.408517045458076 21.08264214176317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1397.0 1 878.5 2 307.5 3 243.5 4 221.0 5 228.5 6 275.0 7 390.0 8 537.0 9 668.5 10 783.0 11 892.0 12 934.0 13 893.5 14 816.5 15 740.0 16 691.5 17 647.5 18 702.0 19 771.0 20 848.5 21 1094.0 22 1439.0 23 1833.0 24 2434.0 25 3409.5 26 4799.5 27 6632.0 28 8470.0 29 10370.0 30 12781.0 31 15477.0 32 20911.5 33 31397.0 34 47421.5 35 70440.0 36 102165.5 37 141940.0 38 182977.5 39 202366.0 40 197760.0 41 176304.5 42 150325.5 43 140836.0 44 134010.5 45 125088.5 46 123667.5 47 110817.0 48 82637.0 49 62214.0 50 50316.0 51 41702.0 52 34833.0 53 27764.5 54 21145.0 55 15456.5 56 11228.5 57 8036.0 58 6088.0 59 4773.0 60 3648.5 61 2782.5 62 2120.5 63 1652.0 64 1331.5 65 1021.0 66 781.5 67 634.0 68 503.5 69 390.0 70 311.0 71 239.0 72 206.5 73 180.0 74 128.5 75 96.5 76 76.0 77 62.5 78 52.5 79 36.5 80 28.0 81 28.0 82 23.0 83 20.0 84 16.0 85 14.0 86 11.5 87 8.5 88 7.5 89 6.5 90 7.0 91 5.5 92 2.5 93 2.5 94 6.0 95 5.5 96 2.0 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2623628754673996 2 0.2491147264938217 3 0.21378632923094704 4 0.2164277047272367 5 0.22984093966933278 6 0.23388554589802638 7 0.2112687682110459 8 0.2064812751240208 9 0.1870836738231434 10-11 0.17501176237525692 12-13 0.20336527746824157 14-15 0.20031118705065662 16-17 0.20159060330667194 18-19 0.19389347002451526 20-21 0.19467762837497626 22-23 0.16069055460631124 24-25 0.4677298203039233 26-27 0.3260860593153885 28-29 0.2779428637462959 30-31 0.2819049269907304 32-33 0.32381612724826453 34-35 0.31999851422628334 36-37 0.3126109171350981 38-39 0.336238846379252 40-41 0.34207876251557995 42-43 0.3771801665717422 44-45 0.34490585972645255 46-47 0.37082435678379516 48-49 0.3467011696340869 50-51 0.3687195106851893 52-53 0.35730794311137526 54-55 0.33308157723134324 56-57 0.3707211780534713 58-59 0.3568333209518857 60-61 0.3230938761359978 62-63 0.3013850712758669 64-65 0.3072868946503892 66-67 0.31686188082443933 68-69 0.32305260464386826 70-71 0.29956912562216775 72-73 0.3071630801740006 74-75 0.3047693336304881 76-77 0.3083186819536273 78-79 0.31042352805223317 80-81 0.3271178466186267 82-83 0.3442042443602506 84-85 0.3389214933676712 86-87 0.34282164937391146 88-89 0.337807163080174 90-91 0.3119299375149609 92-93 0.2973404650471733 94-95 0.3059868426483091 96-97 0.29441018910597694 98-99 0.3134157112316239 100-101 0.3354753237748558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.31436864007651 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37054145592484 38.27714209545092 2 5.188310010008303 4.494567447849974 3 1.8089624721217594 2.35062202120638 4 0.9203384775241583 1.5945552035651265 5 0.6798896450468508 1.472449536006503 6 0.41873956486449987 1.0882463926035706 7 0.3181439708826215 0.9646143664799344 8 0.2455841618634529 0.8509858335294255 9 0.18092910752575972 0.7053147054981718 >10 1.4484225686316112 12.241665268344809 >50 0.20529180085899645 6.181604576892767 >100 0.18154718764024488 17.05363191781038 >500 0.02612678527049067 7.600092401145672 >1k 0.007076514072481021 4.892018994932208 >5k 9.627776394497979E-5 0.232489238684048 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5575 0.23008856862210997 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4664 0.19249023929211137 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4232 0.17466095469215592 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3741 0.15439665205655845 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3638 0.15014568836721723 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3405 0.14052943070103757 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2983 0.1231128610223774 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2963 0.12228743117978687 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2833 0.11692213720294843 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2701 0.11147430024185095 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2683 0.11073141338351948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.079728268495819E-4 0.0 0.0 2 9.079728268495819E-4 0.0 0.0 3 9.492443189791083E-4 0.0 0.0 4 9.492443189791083E-4 0.0 4.127149212952645E-5 5 0.0010730587953676877 0.0 4.127149212952645E-5 6 0.0011143302874972142 0.0 4.127149212952645E-5 7 0.00127941625601532 0.0 4.127149212952645E-5 8 0.001361959240274373 0.0 4.127149212952645E-5 9 0.0014857737166629522 0.0 4.127149212952645E-5 10-11 0.0030128189254554306 0.0 4.127149212952645E-5 12-13 0.003879520260175486 0.0 4.127149212952645E-5 14-15 0.0051589365161908065 0.0 4.127149212952645E-5 16-17 0.006541531502529943 0.0 4.127149212952645E-5 18-19 0.008027305219192895 0.0 4.127149212952645E-5 20-21 0.009760707888633006 0.0 4.127149212952645E-5 22-23 0.011948096971497908 0.0 4.127149212952645E-5 24-25 0.0141973932925571 0.0 4.127149212952645E-5 26-27 0.017045126249494426 0.0 4.127149212952645E-5 28-29 0.020367481365921303 0.0 4.127149212952645E-5 30-31 0.0343997886899603 0.0 4.127149212952645E-5 32-33 0.038423759172589125 0.0 4.127149212952645E-5 34-35 0.0423651866709589 0.0 4.127149212952645E-5 36-37 0.04688441505914205 0.0 4.127149212952645E-5 38-39 0.05165127240010235 0.0 4.127149212952645E-5 40-41 0.057511824282495114 0.0 4.127149212952645E-5 42-43 0.06275330378294497 0.0 6.190723819428968E-5 44-45 0.06871703439566154 0.0 8.25429842590529E-5 46-47 0.0749077582150905 0.0 1.2381447638857936E-4 48-49 0.08136674673336139 0.0 1.2381447638857936E-4 50-51 0.08885752255487045 0.0 1.2381447638857936E-4 52-53 0.0969673707583224 0.0 1.2381447638857936E-4 54-55 0.10505658321570958 0.0 1.2381447638857936E-4 56-57 0.11527127751776738 0.0 1.2381447638857936E-4 58-59 0.1256304220422785 0.0 1.2381447638857936E-4 60-61 0.13671181767905638 0.0 1.2381447638857936E-4 62-63 0.1481440209989352 0.0 1.650859685181058E-4 64-65 0.16151598444890175 0.0 1.650859685181058E-4 66-67 0.17548638453474646 0.0 1.8572171458286903E-4 68-69 0.19009649274859883 0.0 2.0635746064763227E-4 70-71 0.20621301042517892 0.0 2.0635746064763227E-4 72-73 0.22360894435777431 0.0 2.269932067123955E-4 74-75 0.2435843465484651 0.0 2.476289527771587E-4 76-77 0.2647153505187827 0.0 2.476289527771587E-4 78-79 0.2883432797629365 0.0 2.476289527771587E-4 80-81 0.31440622704273247 0.0 2.476289527771587E-4 82-83 0.3427597421357172 0.0 2.476289527771587E-4 84-85 0.37264030243749435 0.0 2.476289527771587E-4 86-87 0.4089798512575424 0.0 2.476289527771587E-4 88-89 0.4467845380481886 0.0 2.476289527771587E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 565 0.0 67.99836 9 CGGGAGA 750 0.0 63.90012 4 AGAGCGG 610 0.0 62.986004 8 CGGAAAG 330 0.0 61.824432 5 GAGAGCG 780 0.0 57.17102 7 AACCGGA 145 0.0 55.639782 2 TCGGGAG 1005 0.0 53.825657 3 TTGTGTT 30505 0.0 51.219845 1 ACCGGAA 140 0.0 50.84097 3 TCGGGAA 180 0.0 50.087765 3 GATCCGG 220 0.0 49.61975 1 GAACCGG 165 0.0 48.900623 1 GATCCGA 90 2.2737368E-10 47.46237 1 GGATCGG 425 0.0 44.670467 1 ATCCGGA 255 0.0 42.80477 2 TCGGAGA 290 0.0 42.541904 4 GTTGACG 45 9.6648035E-4 42.188778 1 ATTCGGA 135 0.0 42.18441 2 GGACCGG 160 0.0 41.529575 1 ATCGGGA 1445 0.0 40.396317 2 >>END_MODULE