##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2652944 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.046507201056635 33.0 33.0 33.0 33.0 33.0 2 32.01629510460831 33.0 33.0 33.0 33.0 33.0 3 31.995495947144004 33.0 33.0 33.0 33.0 33.0 4 35.73873666387229 37.0 37.0 37.0 33.0 37.0 5 35.70378492723555 37.0 37.0 37.0 33.0 37.0 6 35.73320545024697 37.0 37.0 37.0 33.0 37.0 7 35.707570156022896 37.0 37.0 37.0 33.0 37.0 8 35.67734373586476 37.0 37.0 37.0 33.0 37.0 9 35.567901923297285 37.0 37.0 37.0 33.0 37.0 10-11 35.505078320537486 37.0 37.0 37.0 33.0 37.0 12-13 35.513065296515876 37.0 37.0 37.0 33.0 37.0 14-15 37.28214598574263 40.0 37.0 40.0 33.0 40.0 16-17 37.260104057982375 40.0 37.0 40.0 33.0 40.0 18-19 37.268914647274876 40.0 37.0 40.0 33.0 40.0 20-21 37.24321451941692 40.0 37.0 40.0 33.0 40.0 22-23 37.275234984228845 40.0 37.0 40.0 33.0 40.0 24-25 37.08446069724804 40.0 37.0 40.0 33.0 40.0 26-27 36.98718687616474 40.0 37.0 40.0 33.0 40.0 28-29 36.90640586457913 40.0 37.0 40.0 33.0 40.0 30-31 36.9358703010693 40.0 37.0 40.0 33.0 40.0 32-33 36.809118850605216 40.0 37.0 40.0 33.0 40.0 34-35 36.77499694678817 40.0 37.0 40.0 33.0 40.0 36-37 36.662212809618296 40.0 37.0 40.0 33.0 40.0 38-39 36.608574285774594 40.0 37.0 40.0 33.0 40.0 40-41 36.53703018231821 40.0 37.0 40.0 33.0 40.0 42-43 36.40613088704473 40.0 37.0 40.0 33.0 40.0 44-45 36.30816594696307 40.0 37.0 40.0 33.0 40.0 46-47 36.2309875368647 37.0 37.0 40.0 33.0 40.0 48-49 36.106054632136974 37.0 37.0 40.0 33.0 40.0 50-51 34.94333220000121 37.0 35.0 38.5 27.0 38.5 52-53 35.371641090049394 37.0 37.0 38.5 30.0 40.0 54-55 36.15059439626317 37.0 37.0 40.0 33.0 40.0 56-57 36.201983343787134 37.0 37.0 40.0 33.0 40.0 58-59 35.998518438383925 37.0 37.0 40.0 30.0 40.0 60-61 35.72969915686121 37.0 37.0 40.0 27.0 40.0 62-63 35.49638175551387 37.0 37.0 40.0 27.0 40.0 64-65 35.2369397921705 37.0 37.0 40.0 27.0 40.0 66-67 34.990700519875276 37.0 35.0 37.0 27.0 40.0 68-69 34.75563939532836 37.0 33.0 37.0 27.0 40.0 70-71 34.44336367446881 37.0 33.0 37.0 27.0 40.0 72-73 34.21475726589027 37.0 33.0 37.0 27.0 38.5 74-75 33.982168489044625 37.0 33.0 37.0 27.0 37.0 76-77 33.74889858210351 37.0 33.0 37.0 27.0 37.0 78-79 33.49572550344071 37.0 33.0 37.0 27.0 37.0 80-81 33.27750906163115 37.0 33.0 37.0 27.0 37.0 82-83 33.06538415435833 37.0 33.0 37.0 27.0 37.0 84-85 32.93195785512246 37.0 33.0 37.0 27.0 37.0 86-87 32.77851643306455 37.0 33.0 37.0 27.0 37.0 88-89 32.562201463732364 37.0 33.0 37.0 22.0 37.0 90-91 32.51771955985501 33.0 33.0 37.0 22.0 37.0 92-93 32.388302957016805 33.0 33.0 37.0 22.0 37.0 94-95 32.25719238702362 33.0 33.0 37.0 22.0 37.0 96-97 32.100016999981904 33.0 33.0 37.0 22.0 37.0 98-99 31.96329021645387 33.0 33.0 37.0 22.0 37.0 100-101 30.70937268182065 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.3153196557599145 2306 2 0.34470800347330055 2306 3 0.25521680077343234 2306 4 0.33488261831576693 2306 5 0.38512034649092186 2306 6 0.4130590390621407 2306 7 0.42844234082378563 2306 8 0.4426166837833492 2306 9 0.40093533156074557 2306 10-11 0.3890952782832997 2306 12-13 0.4289836635557336 2306 14-15 0.5486365479512472 2306 16-17 0.49790893568214756 2306 18-19 0.4975062633175469 2306 20-21 0.6020369870612541 2306 22-23 0.5752070322754506 2306 24-25 0.6189209641837792 2306 26-27 0.7309120016091342 2306 28-29 0.5826223342586019 2306 30-31 0.46912272573217706 2306 32-33 0.5287448234997782 2306 34-35 0.5183580788749538 2306 36-37 0.48810893153287793 2306 38-39 0.4719237129535827 2306 40-41 0.3578097754035099 2306 42-43 0.368125940473675 2306 44-45 0.40941894772596044 2306 46-47 0.39123863017290006 2306 48-49 0.3119565519860501 2306 50-51 0.42125654270184043 2306 52-53 0.46190684180218256 2306 54-55 0.4436121307962253 2306 56-57 0.4600975416829698 2306 58-59 0.4850710447590316 2306 60-61 0.37971551743186893 2306 62-63 0.34526849307814445 2306 64-65 0.41891692390692015 2306 66-67 0.3919226843157446 2306 68-69 0.4626115587727071 2306 70-71 0.6093776292387787 2306 72-73 0.4813145867126565 2306 74-75 0.5361043232273985 2306 76-77 0.4861168000813123 2306 78-79 0.5189964933774363 2306 80-81 0.44190760996217193 2306 82-83 0.4247403316693621 2306 84-85 0.47816786383871346 2306 86-87 0.4462014474374527 2306 88-89 0.5367298357781536 2306 90-91 0.3462159507906293 2306 92-93 0.2572235342155693 2306 94-95 0.29763910767881185 2306 96-97 0.41867073687429013 2306 98-99 0.4299890638115045 2306 100-101 0.21171608862585245 2307 1 0.2941548503626592 2307 2 0.28965915047524504 2307 3 0.26698240860353906 2307 4 0.38346515730872 2307 5 0.36888556247483706 2307 6 0.44113055951054747 2307 7 0.38641896634660355 2307 8 0.3811841452163094 2307 9 0.4047213324296237 2307 10-11 0.4376903819095759 2307 12-13 0.27838737587247664 2307 14-15 0.48064952998594634 2307 16-17 0.5096293960162441 2307 18-19 0.46777386871139015 2307 20-21 0.4089364614762516 2307 22-23 0.4970036896570704 2307 24-25 0.46142924832337684 2307 26-27 0.3861009822127457 2307 28-29 0.4030781961924461 2307 30-31 0.36751530450604264 2307 32-33 0.38935831834189116 2307 34-35 0.4843030244850013 2307 36-37 0.42753440835006273 2307 38-39 0.2784669054778419 2307 40-41 0.2942124995863793 2307 42-43 0.2956993477466838 2307 44-45 0.33356157122139507 2307 46-47 0.2565657791511242 2307 48-49 0.2010557742565382 2307 50-51 0.25990940104944826 2307 52-53 0.27328039048055786 2307 54-55 0.2589923067862685 2307 56-57 0.11353322353793516 2307 58-59 0.12306607001607972 2307 60-61 0.23237858081724738 2307 62-63 0.25502269477672 2307 64-65 0.19280974545505813 2307 66-67 0.18699436661366775 2307 68-69 0.18594514765790393 2307 70-71 0.10704869991590726 2307 72-73 -0.0442821268903657 2307 74-75 0.1293326247445279 2307 76-77 0.3047686788134314 2307 78-79 0.30349258769310694 2307 80-81 0.07357517106036227 2307 82-83 0.26864329010810906 2307 84-85 0.2842206097434925 2307 86-87 0.29248976956909445 2307 88-89 0.38112579635501476 2307 90-91 0.3547098558786672 2307 92-93 0.3671868625230488 2307 94-95 0.2732309232044443 2307 96-97 0.24663961065722617 2307 98-99 0.23896021200603457 2307 100-101 -0.08711689119406785 2201 1 0.2964996657064347 2201 2 0.29273621630920843 2201 3 0.3229707248711975 2201 4 0.4170014228715644 2201 5 0.3870312540985381 2201 6 0.4472838842908686 2201 7 0.3680723597123716 2201 8 0.42849801642091023 2201 9 0.45114197906934095 2201 10-11 0.44865618601340174 2201 12-13 0.4488742518457869 2201 14-15 0.6688516230523618 2201 16-17 0.571788788591256 2201 18-19 0.6048570104089208 2201 20-21 0.5893839613350522 2201 22-23 0.47873192184704294 2201 24-25 0.5153448720878302 2201 26-27 0.6118814442365803 2201 28-29 0.37682187221093955 2201 30-31 0.3961658656963607 2201 32-33 0.4734117988515223 2201 34-35 0.4079155812697195 2201 36-37 0.5421429064901915 2201 38-39 0.6164961228775638 2201 40-41 0.43598365819627816 2201 42-43 0.4244009445772292 2201 44-45 0.4333806266787761 2201 46-47 0.35576373288419205 2201 48-49 0.4664852805220292 2201 50-51 0.42541031686686637 2201 52-53 0.44976829120110295 2201 54-55 0.5208642456564689 2201 56-57 0.5155365237081853 2201 58-59 0.33273863503104195 2201 60-61 0.44486093015116523 2201 62-63 0.43355242455006504 2201 64-65 0.449266056526362 2201 66-67 0.4080202434987612 2201 68-69 0.3957193805095187 2201 70-71 0.47573904066453565 2201 72-73 0.4379633202463964 2201 74-75 0.4049989121740225 2201 76-77 0.37051791428744707 2201 78-79 0.37396534313355545 2201 80-81 0.526936261326874 2201 82-83 0.4520222125904141 2201 84-85 0.3462484796409413 2201 86-87 0.4856782262351942 2201 88-89 0.5831912268698929 2201 90-91 0.5045781314791924 2201 92-93 0.5082959698633047 2201 94-95 0.4972842423365549 2201 96-97 0.5091019895232165 2201 98-99 0.3471775552479457 2201 100-101 0.19162767240077727 1101 1 0.2858477265232935 1101 2 0.22374949311740266 1101 3 0.2532114459535535 1101 4 0.3040029167827072 1101 5 0.32581673733270833 1101 6 0.32660476343425415 1101 7 0.3731332254981652 1101 8 0.2982029092359184 1101 9 0.34513914143460767 1101 10-11 0.36723006407247993 1101 12-13 0.34422424006971397 1101 14-15 0.5707128679479752 1101 16-17 0.5226518550799781 1101 18-19 0.4380933476073068 1101 20-21 0.4546133785276609 1101 22-23 0.40162852794943404 1101 24-25 0.38288968088881603 1101 26-27 0.4417286056347365 1101 28-29 0.3922712800509629 1101 30-31 0.42462925349543923 1101 32-33 0.40099038847652224 1101 34-35 0.3555126371383537 1101 36-37 0.48886927271345826 1101 38-39 0.40736648256540775 1101 40-41 0.3524702704540843 1101 42-43 0.4192612872096717 1101 44-45 0.5074071666417552 1101 46-47 0.22366267713388055 1101 48-49 0.2620094635642616 1101 50-51 0.36233580676845634 1101 52-53 0.39775560717867364 1101 54-55 0.34175704245610916 1101 56-57 0.4823189030660515 1101 58-59 0.4005818847258311 1101 60-61 0.4715141026927867 1101 62-63 0.5024663295291205 1101 64-65 0.46339478092193787 1101 66-67 0.5008685847795178 1101 68-69 0.3763275491200915 1101 70-71 0.3795999419492091 1101 72-73 0.3812774705318063 1101 74-75 0.5720319454735616 1101 76-77 0.48716301993643185 1101 78-79 0.3635787922023255 1101 80-81 0.5177165084764468 1101 82-83 0.5167211946145542 1101 84-85 0.4305540498928835 1101 86-87 0.48350050656028287 1101 88-89 0.4653998032624571 1101 90-91 0.5025210169691405 1101 92-93 0.3881703812020092 1101 94-95 0.5071272166874721 1101 96-97 0.4103183950114442 1101 98-99 0.44589316713142324 1101 100-101 0.3327277507941311 2304 1 0.21310518865817585 2304 2 0.20216489165698448 2304 3 0.261927466941664 2304 4 0.3935686722628091 2304 5 0.47258712909377465 2304 6 0.4466289712777467 2304 7 0.43830863530717323 2304 8 0.40784735109219383 2304 9 0.41993999908599733 2304 10-11 0.4985257769221789 2304 12-13 0.4142611740839328 2304 14-15 0.5715288667491905 2304 16-17 0.5824820010653937 2304 18-19 0.5331263676210511 2304 20-21 0.5258033779888578 2304 22-23 0.4732237148989711 2304 24-25 0.5452579314603199 2304 26-27 0.5716140991244387 2304 28-29 0.5392647199503244 2304 30-31 0.41760446168126464 2304 32-33 0.47620840692012223 2304 34-35 0.49111746949122903 2304 36-37 0.5292856135157749 2304 38-39 0.5509973289165302 2304 40-41 0.5120575397843083 2304 42-43 0.4870789874899444 2304 44-45 0.4610790070455977 2304 46-47 0.42264764936760457 2304 48-49 0.5588287553868696 2304 50-51 0.5520565258270338 2304 52-53 0.5773745767380021 2304 54-55 0.5538095152789779 2304 56-57 0.49869212400997043 2304 58-59 0.6579602971854754 2304 60-61 0.5599948228616185 2304 62-63 0.5008040358395789 2304 64-65 0.4774224134931728 2304 66-67 0.6046123647842094 2304 68-69 0.6688005316320016 2304 70-71 0.531715325413316 2304 72-73 0.4188233024342338 2304 74-75 0.36567305076916057 2304 76-77 0.5622862497242664 2304 78-79 0.5406937085261703 2304 80-81 0.47146762666510966 2304 82-83 0.5218890358441968 2304 84-85 0.6053236499645749 2304 86-87 0.5114842039999701 2304 88-89 0.4771630300896277 2304 90-91 0.6199162682479695 2304 92-93 0.6425844031744745 2304 94-95 0.47023392984684875 2304 96-97 0.5390355843037469 2304 98-99 0.35988185018229046 2304 100-101 0.4268844519529864 2202 1 0.20499137979211923 2202 2 0.11888755779835947 2202 3 0.11858169426213294 2202 4 0.10433829487884339 2202 5 0.13082887554869416 2202 6 0.23799918974976464 2202 7 0.25474011553034615 2202 8 0.1278333493790953 2202 9 0.12092340491076925 2202 10-11 0.23434840395614032 2202 12-13 0.22907906924032062 2202 14-15 0.3450735458173 2202 16-17 0.25326399776348296 2202 18-19 0.30956664658612 2202 20-21 0.20360674687866265 2202 22-23 0.3504739446145706 2202 24-25 0.3658666859020414 2202 26-27 0.432798452686761 2202 28-29 0.32194039216109616 2202 30-31 0.32288288959397704 2202 32-33 0.41650533381309884 2202 34-35 0.38373357483233406 2202 36-37 0.30004850768213487 2202 38-39 0.3088122138067533 2202 40-41 0.24593458587912664 2202 42-43 0.27853092802283896 2202 44-45 0.3803432292805695 2202 46-47 0.35444224709672056 2202 48-49 0.40806227907018666 2202 50-51 0.3986316989713927 2202 52-53 0.37249745522053246 2202 54-55 0.5670770067368949 2202 56-57 0.5533058706480816 2202 58-59 0.4287071023210913 2202 60-61 0.4019166907827625 2202 62-63 0.4432860914626744 2202 64-65 0.4608008273258619 2202 66-67 0.49488219570637426 2202 68-69 0.5557443598031924 2202 70-71 0.5806765443795712 2202 72-73 0.6179711115934765 2202 74-75 0.6132040655681763 2202 76-77 0.5923579250792912 2202 78-79 0.5932870474631429 2202 80-81 0.4680444087125437 2202 82-83 0.4608605330621529 2202 84-85 0.4573542814360465 2202 86-87 0.5146104984646485 2202 88-89 0.6873893712550583 2202 90-91 0.6445504471038532 2202 92-93 0.4572322142916363 2202 94-95 0.45383529167688863 2202 96-97 0.5240028963306713 2202 98-99 0.6966765100542176 2202 100-101 0.80650768601161 1102 1 0.13724940074022385 1102 2 0.16611525432536212 1102 3 0.12890034517646853 1102 4 0.20316705672697566 1102 5 0.24863401590054224 1102 6 0.20416107046106902 1102 7 0.2683877729482731 1102 8 0.13813589062269926 1102 9 0.28563437382285173 1102 10-11 0.18777347454172144 1102 12-13 0.2522512847122087 1102 14-15 0.3157100096176464 1102 16-17 0.22701337066607152 1102 18-19 0.28137569914709104 1102 20-21 0.238764964185755 1102 22-23 0.2365392746992896 1102 24-25 0.29825293332022085 1102 26-27 0.3215585726924175 1102 28-29 0.18655862469589124 1102 30-31 0.11819959677076497 1102 32-33 0.15129377722348636 1102 34-35 0.15392570462356048 1102 36-37 0.17583944776603744 1102 38-39 0.14789979134674525 1102 40-41 0.0744933545274904 1102 42-43 0.07083602175427473 1102 44-45 0.196559712012224 1102 46-47 0.22949897673267117 1102 48-49 0.16818106429686708 1102 50-51 0.08168548070032244 1102 52-53 0.11160084952815197 1102 54-55 0.22151236521855822 1102 56-57 0.2352993431396797 1102 58-59 0.28000637981049437 1102 60-61 0.27206688661289746 1102 62-63 0.26219752683922337 1102 64-65 0.2957418732535544 1102 66-67 0.27993785624599354 1102 68-69 0.25716300344377174 1102 70-71 0.32409163510550343 1102 72-73 0.22007648283432957 1102 74-75 0.36920805035217796 1102 76-77 0.13991879888736491 1102 78-79 0.2519941536341648 1102 80-81 0.3742751411802132 1102 82-83 0.29283772502287775 1102 84-85 0.2677959353817698 1102 86-87 0.09745789741185718 1102 88-89 0.1239031929457326 1102 90-91 0.28137207741229275 1102 92-93 0.2581402790043441 1102 94-95 0.2388506780396753 1102 96-97 0.19129954875735677 1102 98-99 0.17423630825699377 1102 100-101 0.19678748495640974 2305 1 0.28940638000684515 2305 2 0.22514085514909965 2305 3 0.21454741403990596 2305 4 0.29891545186738 2305 5 0.30578853932941996 2305 6 0.371586290435296 2305 7 0.2894402356847863 2305 8 0.31071214072508724 2305 9 0.3390347513709173 2305 10-11 0.39118333595273924 2305 12-13 0.3346864541774721 2305 14-15 0.44867516973609156 2305 16-17 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-0.9668731192077331 1213 100-101 -0.9311036091710925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1976.0 3 255.0 4 203.0 5 184.0 6 316.0 7 1531.0 8 4736.0 9 9243.0 10 12390.0 11 10520.0 12 7632.0 13 6038.0 14 5373.0 15 5432.0 16 5333.0 17 5714.0 18 6292.0 19 6897.0 20 7955.0 21 8997.0 22 10258.0 23 11094.0 24 12556.0 25 14507.0 26 16979.0 27 20435.0 28 24484.0 29 30631.0 30 38465.0 31 50494.0 32 69949.0 33 99368.0 34 151530.0 35 255125.0 36 510367.0 37 939090.0 38 289635.0 39 960.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.554846234464996 25.12287976887832 23.98673559902021 14.335538397636475 2 32.87183136220674 22.162259800240864 32.505620138707556 12.460288698844835 3 39.04226433226404 18.52386859692571 26.03092121466703 16.40294585614322 4 31.67980586052496 17.798108092078746 33.82847865021085 16.693607397185446 5 30.962945555197045 16.66214540527756 35.472960464024986 16.901948575500406 6 25.216131305364698 17.24830021291026 40.60408326736957 16.93148521435548 7 33.795956851017245 17.180342334603687 30.226801491183636 18.79689932319543 8 30.745506431794272 26.398705142224717 24.943614383195555 17.912174042785455 9 29.74860229705172 26.78963381013597 24.80169310506829 18.660070787744022 10-11 24.53993614265137 28.15944437196462 30.163770630868385 17.13684885451562 12-13 25.177411849313618 25.810440141536656 29.81185732113773 19.20029068801199 14-15 23.47289962203262 26.822690733397213 30.840657308949332 18.863752335620827 16-17 22.57587629617501 27.941649438471313 31.610721537028862 17.871752728324815 18-19 22.10376933601108 28.01807951853415 31.527911932689957 18.350239212764812 20-21 22.087859316019454 28.359515908300782 31.08331068807652 18.469314087603248 22-23 22.218769715314167 27.750063334414392 31.12913839900417 18.902028551267275 24-25 22.159388845996304 27.883626830344017 31.017966974792603 18.939017348867075 26-27 21.794826218204673 28.29756544377875 31.015763689131365 18.89184464888521 28-29 21.339902632255335 28.581893228103596 30.46671126835095 19.611492871290125 30-31 21.263047440634004 28.876527194090578 30.331656547741897 19.528768817533525 32-33 20.71214862232118 29.175559511852754 31.337995680005807 18.774296185820255 34-35 21.42762878271089 28.79210763946135 31.222235162970236 18.558028414857525 36-37 20.917767777267755 29.153089474484204 31.498827490193793 18.430315258054247 38-39 21.26871260032813 28.988296091561732 30.84925005881076 18.893741249299374 40-41 21.295172839487496 28.924819475059344 30.072191065206834 19.707816620246327 42-43 21.322286814116286 28.79425668779603 30.694404795754227 19.18905170233346 44-45 20.77426219227595 28.85194523432908 31.28990871631706 19.083883857077907 46-47 21.07469916621756 28.72099320689673 30.886648376936176 19.31765924994954 48-49 20.852030361572016 29.721830583033338 29.911765345558152 19.514373709836494 50-51 20.934686218428897 29.239626367639715 29.75335194685311 20.072335467078283 52-53 21.26443108091392 28.87948725805396 29.76098471995409 20.095096941078026 54-55 21.894905316757676 28.980618975753536 29.447686286582293 19.676789420906502 56-57 21.95984961339292 29.210678394930362 29.154193563642384 19.675278428034336 58-59 21.51443719426609 29.22893253034335 29.80925362672314 19.44737664866742 60-61 20.803296895698008 29.47460829888668 29.689097108749834 20.03299769666548 62-63 21.13495614491921 29.262593059368015 29.412573106766548 20.18987768894622 64-65 20.7666168532072 29.227002864982836 29.65664631593076 20.3497339658792 66-67 21.18264385824972 28.396584976566473 29.755368327674965 20.665402837508843 68-69 21.209927364803544 28.79538565326299 30.128215793995572 19.866471187937897 70-71 20.527472859835203 30.27626920321429 29.43901189573791 19.757246041212596 72-73 20.366428445579047 30.49464966679043 29.009025733473177 20.12989615415735 74-75 20.95264308852842 29.709376710816443 29.06575055124207 20.272229649413067 76-77 20.81928328545116 29.40606886450155 29.311470302497995 20.463177547549293 78-79 20.722142694558567 29.51110904414806 29.50628823093926 20.260460030354107 80-81 20.015694001015383 29.979285809503192 29.489954421217533 20.51506576826389 82-83 19.472214578776814 29.640423960641144 29.673859700383243 21.2135017601988 84-85 19.65608572668021 29.3218006899314 30.068482555613002 20.953631027775394 86-87 19.98235944002569 29.31353107352112 30.640563378363915 20.063546108089277 88-89 20.189927539493226 30.002825199569905 29.865895680924154 19.941351580012714 90-91 20.42218732258326 29.601472968944048 29.51359998850543 20.462739719967264 92-93 19.843173888601783 30.21729506619451 29.684151311887113 20.255379733316598 94-95 19.463272991243592 30.620585877833733 29.821639217821332 20.094501913101343 96-97 19.820812940451745 31.131818868303995 29.324637663079255 19.722730528165002 98-99 20.476491794706106 30.245237171116624 28.78565280215281 20.492618232024455 100-101 20.45429876643222 30.05581443195257 28.19958178886805 21.290305012747158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1554.0 1 981.0 2 358.0 3 290.0 4 260.0 5 258.0 6 324.5 7 463.5 8 621.5 9 793.0 10 915.5 11 993.5 12 1040.5 13 992.5 14 868.0 15 793.5 16 742.0 17 699.5 18 689.5 19 702.5 20 820.0 21 1034.5 22 1332.0 23 1745.5 24 2343.5 25 3348.0 26 5305.5 27 8240.0 28 11191.5 29 13969.0 30 17447.5 31 20966.5 32 26544.5 33 37662.0 34 54255.0 35 78539.0 36 113326.5 37 151168.0 38 185258.5 39 205317.5 40 203505.0 41 185930.5 42 167274.0 43 158798.5 44 147353.0 45 133209.0 46 124898.0 47 110750.5 48 91956.0 49 77270.5 50 64039.0 51 52594.0 52 42740.0 53 33978.0 54 27922.0 55 22047.5 56 15793.0 57 10612.0 58 7401.0 59 5452.0 60 3986.5 61 2953.0 62 2178.0 63 1593.0 64 1216.5 65 944.0 66 692.0 67 506.0 68 405.0 69 310.0 70 239.5 71 203.5 72 146.0 73 98.0 74 79.5 75 71.5 76 67.5 77 60.0 78 50.0 79 37.0 80 26.0 81 27.5 82 25.5 83 18.0 84 17.5 85 19.5 86 12.0 87 10.0 88 12.0 89 9.0 90 6.5 91 5.5 92 5.5 93 3.5 94 3.0 95 2.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2663079205591976 2 0.2509287794993034 3 0.21485564716028685 4 0.217682695149238 5 0.23332569402143433 6 0.23705739736684983 7 0.21300864247417206 8 0.20916385720919853 9 0.1895252971792846 10-11 0.17706743904130656 12-13 0.20475366234643474 14-15 0.2020773902502277 16-17 0.2033401383519592 18-19 0.19587673166112815 20-21 0.19685677496396456 22-23 0.1622725545657956 24-25 0.47598064640640736 26-27 0.3304253689486095 28-29 0.2753167801506553 30-31 0.2791615654156288 32-33 0.32024799618838545 34-35 0.31708170244076017 36-37 0.30871364039346477 38-39 0.3343455421599551 40-41 0.3387934309959049 42-43 0.37465924648239846 44-45 0.3429397680463666 46-47 0.36745969760386954 48-49 0.3445794558799583 50-51 0.3657823157970918 52-53 0.3554918611173097 54-55 0.33116040142573683 56-57 0.36776124938935767 58-59 0.35460605274743834 60-61 0.32103956962529173 62-63 0.29874358448576377 64-65 0.30443537443685204 66-67 0.31419811349203003 68-69 0.32024799618838545 70-71 0.296990814732614 72-73 0.3038888118256548 74-75 0.30185333727361 76-77 0.30469923224915413 78-79 0.30765820914425634 80-81 0.32497858982323036 82-83 0.34175240789100714 84-85 0.3359663830069538 86-87 0.3406404356820197 88-89 0.3348355638113733 90-91 0.3097502246560802 92-93 0.2934475812531286 94-95 0.3024564408445863 96-97 0.29097862600944463 98-99 0.3097879186292662 100-101 0.33264931336658443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2652944.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.18423490085504 #Duplication Level Percentage of deduplicated Percentage of total 1 87.749527427262 42.28143841994214 2 5.378696220431978 5.183367242912712 3 1.9825344902880828 2.865807227372637 4 1.0396764530634208 2.003840577411826 5 0.5991204116548211 1.4434079324536435 6 0.4844847611356223 1.4006716521866078 7 0.35894717742442966 1.2106916579812326 8 0.26462570500409677 1.0200629704577417 9 0.2225308387799793 0.9650230387612942 >10 1.5951347496152988 15.344917972724812 >50 0.17645834577491962 5.868992161751698 >100 0.13104045474253417 13.528038762297784 >500 0.014430018661841062 4.469943337454177 >1k 0.0026352314321476866 1.9592411163182684 >5k 1.5771472874527654E-4 0.45455592997378286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6071 0.22884011121229847 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5892 0.22209289001200178 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2892 0.10901097045395605 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2832 0.10674933206279513 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2810 0.1059200646527028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.523276782321828E-4 0.0 0.0 2 4.90021651418198E-4 0.0 0.0 3 6.40797544162259E-4 0.0 0.0 4 6.784915173482742E-4 0.0 0.0 5 7.161854905342894E-4 0.0 0.0 6 7.915734369063199E-4 0.0 0.0 7 8.669613832783504E-4 0.0 0.0 8 9.80043302836396E-4 0.0 0.0 9 0.0012439011151385026 0.0 0.0 10-11 0.0019412396190797848 0.0 0.0 12-13 0.0028270479889511424 0.0 0.0 14-15 0.0037882443051945307 0.0 0.0 16-17 0.004919063500774989 0.0 0.0 18-19 0.0063702814684365745 0.0 0.0 20-21 0.008104204234993275 0.0 0.0 22-23 0.01004544385407306 0.0 0.0 24-25 0.012646328003908112 0.0 0.0 26-27 0.01575608079175437 0.0 0.0 28-29 0.01914853837849574 0.0 0.0 30-31 0.028835889487301654 0.0 0.0 32-33 0.032567592832717165 0.0 0.0 34-35 0.03680816481614388 0.0 0.0 36-37 0.041161818719128634 0.0 0.0 38-39 0.04564740152826445 0.0 0.0 40-41 0.050566465029039435 0.0 0.0 42-43 0.05608863210079067 0.0 0.0 44-45 0.06215736178373912 0.0 0.0 46-47 0.06884804202425682 0.0 0.0 48-49 0.07634914268827386 0.0 0.0 50-51 0.08383139636569789 0.0 0.0 52-53 0.09231254033255132 0.0 0.0 54-55 0.10085022525918376 0.0 0.0 56-57 0.1095952270383393 3.769397318601523E-5 0.0 58-59 0.12030031542316763 3.769397318601523E-5 0.0 60-61 0.1333235831589359 3.769397318601523E-5 0.0 62-63 0.14613953404218105 3.769397318601523E-5 0.0 64-65 0.15999206918804165 3.769397318601523E-5 0.0 66-67 0.1746738717439946 3.769397318601523E-5 0.0 68-69 0.19193771146318958 3.769397318601523E-5 0.0 70-71 0.2120851401311147 3.769397318601523E-5 0.0 72-73 0.23417380841811963 3.769397318601523E-5 0.0 74-75 0.2588256668817736 3.769397318601523E-5 3.769397318601523E-5 76-77 0.2848722023533101 3.769397318601523E-5 3.769397318601523E-5 78-79 0.31485775802278526 3.769397318601523E-5 3.769397318601523E-5 80-81 0.3495173663673263 3.769397318601523E-5 3.769397318601523E-5 82-83 0.3855904987063429 3.769397318601523E-5 3.769397318601523E-5 84-85 0.4285616281384002 3.769397318601523E-5 3.769397318601523E-5 86-87 0.4785061426098704 3.769397318601523E-5 3.769397318601523E-5 88-89 0.5323708302926862 3.769397318601523E-5 3.769397318601523E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 540 0.0 67.67351 2 GAGCGGC 415 0.0 59.439045 9 CGGGAGA 470 0.0 54.519516 4 AGAGCGG 465 0.0 54.070965 8 CGGAAAG 285 0.0 49.944935 5 TCGGGAA 260 0.0 49.278183 3 CGGGTCG 50 2.839492E-5 47.46742 1 CGGACGG 50 2.839492E-5 47.46742 1 CGTTCGA 40 5.416294E-4 47.45217 4 AAGCGGC 225 0.0 46.38278 9 CGGACCG 45 9.659913E-4 42.193264 1 ATTCGGA 125 3.6379788E-12 41.76423 2 AACGGGT 70 4.350184E-6 40.662533 8 TTGTGTT 33650 0.0 39.29992 1 GAGAGCG 660 0.0 38.815704 7 ACTGGCG 210 0.0 38.41947 2 GGTACGG 100 3.0517185E-8 37.973934 1 GAACCGG 100 3.0517185E-8 37.973934 1 CCGGATC 230 0.0 37.159306 60-61 GGTCCGG 90 5.732436E-7 36.919106 1 >>END_MODULE