FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652944
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60710.22884011121229847No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC58920.22209289001200178No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28920.10901097045395605No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA28320.10674933206279513No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA28100.1059200646527028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5400.067.673512
GAGCGGC4150.059.4390459
CGGGAGA4700.054.5195164
AGAGCGG4650.054.0709658
CGGAAAG2850.049.9449355
TCGGGAA2600.049.2781833
CGGGTCG502.839492E-547.467421
CGGACGG502.839492E-547.467421
CGTTCGA405.416294E-447.452174
AAGCGGC2250.046.382789
CGGACCG459.659913E-442.1932641
ATTCGGA1253.6379788E-1241.764232
AACGGGT704.350184E-640.6625338
TTGTGTT336500.039.299921
GAGAGCG6600.038.8157047
ACTGGCG2100.038.419472
GGTACGG1003.0517185E-837.9739341
GAACCGG1003.0517185E-837.9739341
CCGGATC2300.037.15930660-61
GGTCCGG905.732436E-736.9191061
Produced by FastQC (version 0.11.2)