##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4816329 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66469607869396 33.0 33.0 33.0 27.0 33.0 2 31.55636170203489 33.0 33.0 33.0 27.0 33.0 3 31.49303982348382 33.0 33.0 33.0 27.0 33.0 4 35.10752899147878 37.0 37.0 37.0 33.0 37.0 5 35.088339480131026 37.0 37.0 37.0 33.0 37.0 6 35.218132523754086 37.0 37.0 37.0 33.0 37.0 7 35.06311238289577 37.0 37.0 37.0 33.0 37.0 8 35.15316582401244 37.0 37.0 37.0 33.0 37.0 9 34.906355442080475 37.0 37.0 37.0 33.0 37.0 10-11 34.854377680594496 37.0 37.0 37.0 33.0 37.0 12-13 34.855741582437574 37.0 37.0 37.0 33.0 37.0 14-15 36.50886266282889 40.0 37.0 40.0 33.0 40.0 16-17 36.4683855691752 40.0 37.0 40.0 33.0 40.0 18-19 36.49686400160786 40.0 37.0 40.0 33.0 40.0 20-21 36.47746437172378 40.0 37.0 40.0 33.0 40.0 22-23 36.6528313368958 40.0 37.0 40.0 33.0 40.0 24-25 36.34731960794207 40.0 37.0 40.0 33.0 40.0 26-27 36.251596080749465 40.0 37.0 40.0 30.0 40.0 28-29 36.09491544286115 40.0 37.0 40.0 27.0 40.0 30-31 36.1036941205636 40.0 37.0 40.0 27.0 40.0 32-33 35.97235612434284 40.0 37.0 40.0 27.0 40.0 34-35 35.92837459816387 40.0 37.0 40.0 27.0 40.0 36-37 35.8118909650898 38.5 37.0 40.0 27.0 40.0 38-39 35.71998486814336 37.0 37.0 40.0 27.0 40.0 40-41 35.681275407057946 37.0 37.0 40.0 27.0 40.0 42-43 35.52975824533581 37.0 37.0 40.0 27.0 40.0 44-45 35.39722276032223 37.0 37.0 40.0 27.0 40.0 46-47 35.3205664521672 37.0 37.0 40.0 27.0 40.0 48-49 35.20112475705044 37.0 37.0 40.0 27.0 40.0 50-51 34.10508553298581 37.0 35.0 38.5 24.5 38.5 52-53 34.54423265105021 37.0 35.0 38.5 27.0 40.0 54-55 35.268638624977655 37.0 37.0 40.0 27.0 40.0 56-57 35.368117190499234 37.0 37.0 40.0 27.0 40.0 58-59 35.10171969149117 37.0 37.0 40.0 27.0 40.0 60-61 34.86814490870536 37.0 35.0 40.0 27.0 40.0 62-63 34.614777042847365 37.0 33.0 40.0 27.0 40.0 64-65 34.377204298128305 37.0 33.0 40.0 27.0 40.0 66-67 34.151129003022845 37.0 33.0 37.0 27.0 40.0 68-69 33.98385866081823 37.0 33.0 37.0 27.0 40.0 70-71 33.63612836664605 37.0 33.0 37.0 22.0 40.0 72-73 33.509594963300884 37.0 33.0 37.0 24.5 38.5 74-75 33.33077775625378 37.0 33.0 37.0 27.0 37.0 76-77 33.06909692008166 37.0 33.0 37.0 22.0 37.0 78-79 32.752483893853594 37.0 33.0 37.0 22.0 37.0 80-81 32.46032341229181 37.0 33.0 37.0 22.0 37.0 82-83 32.213430602436006 37.0 33.0 37.0 22.0 37.0 84-85 32.06732077480588 37.0 33.0 37.0 22.0 37.0 86-87 31.912474625383773 33.0 33.0 37.0 22.0 37.0 88-89 31.706634762699974 33.0 33.0 37.0 15.0 37.0 90-91 31.631964822170577 33.0 33.0 37.0 15.0 37.0 92-93 31.476994511795187 33.0 33.0 37.0 15.0 37.0 94-95 31.33500929857574 33.0 33.0 37.0 15.0 37.0 96-97 31.185175265227937 33.0 33.0 37.0 15.0 37.0 98-99 31.043417299773335 33.0 33.0 37.0 15.0 37.0 100-101 29.83482741731306 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21215044690777418 2306 2 0.21538806630591978 2306 3 0.1582525475099139 2306 4 0.22141178078920376 2306 5 0.25595804502344066 2306 6 0.2656579239191501 2306 7 0.34437673483897413 2306 8 0.21146598658540938 2306 9 0.33790996229047465 2306 10-11 0.27287486827593455 2306 12-13 0.2995522515920328 2306 14-15 0.5021695327305196 2306 16-17 0.4359337666925711 2306 18-19 0.4659048530838774 2306 20-21 0.5022900303469982 2306 22-23 0.3954892793939564 2306 24-25 0.4956048796119106 2306 26-27 0.4562581662301213 2306 28-29 0.45848540659563497 2306 30-31 0.3755776100927548 2306 32-33 0.3567733177282193 2306 34-35 0.42589383215540977 2306 36-37 0.31551335624345 2306 38-39 0.4216296622832587 2306 40-41 0.373738229246797 2306 42-43 0.32570040804351663 2306 44-45 0.3900937848965924 2306 46-47 0.3954047217569965 2306 48-49 0.40437387740839625 2306 50-51 0.4336488438660382 2306 52-53 0.4662581759001938 2306 54-55 0.5005281310650957 2306 56-57 0.49224234309261305 2306 58-59 0.5031647000672237 2306 60-61 0.4436988811567275 2306 62-63 0.3973771433899955 2306 64-65 0.5789901492527534 2306 66-67 0.510065559998786 2306 68-69 0.4297170936476178 2306 70-71 0.43421002752403837 2306 72-73 0.3451067921307853 2306 74-75 0.4401554738939595 2306 76-77 0.5315241456725701 2306 78-79 0.43623734154841287 2306 80-81 0.3978021367729667 2306 82-83 0.4278778292598133 2306 84-85 0.5447616643457138 2306 86-87 0.5423541676346559 2306 88-89 0.5459924672584151 2306 90-91 0.3205579872058344 2306 92-93 0.33876921919289416 2306 94-95 0.2930203143241492 2306 96-97 0.3432359673569927 2306 98-99 0.2749019998490354 2306 100-101 0.3012835491111403 2307 1 0.22726745644197166 2307 2 0.2608278236387598 2307 3 0.27975767070680035 2307 4 0.39925964769138034 2307 5 0.3929717672173325 2307 6 0.314616071168615 2307 7 0.41861192276051185 2307 8 0.3649666899084778 2307 9 0.4095152306475569 2307 10-11 0.40796317702352525 2307 12-13 0.31235462431658334 2307 14-15 0.4645769443005179 2307 16-17 0.5281572258590188 2307 18-19 0.5474702171733483 2307 20-21 0.504593428184144 2307 22-23 0.5150624823709791 2307 24-25 0.545559746264459 2307 26-27 0.5411479000794159 2307 28-29 0.5273318029669767 2307 30-31 0.42508942461894605 2307 32-33 0.5117202993542733 2307 34-35 0.4818449863978884 2307 36-37 0.45312925075327115 2307 38-39 0.4190949782226099 2307 40-41 0.4598612924464973 2307 42-43 0.5470531544343302 2307 44-45 0.518128159589736 2307 46-47 0.4683184431227616 2307 48-49 0.3887315937911424 2307 50-51 0.43898972190189056 2307 52-53 0.46983867592799555 2307 54-55 0.38958882506572934 2307 56-57 0.3997757672052984 2307 58-59 0.32669626915792094 2307 60-61 0.40402353366544475 2307 62-63 0.4479974755193794 2307 64-65 0.4030067687354517 2307 66-67 0.4084924587640373 2307 68-69 0.4993916248345798 2307 70-71 0.4136829315443933 2307 72-73 0.34523692760181035 2307 74-75 0.4475193669793853 2307 76-77 0.4032064305675078 2307 78-79 0.5337443008384852 2307 80-81 0.3810779541716016 2307 82-83 0.4706746534810975 2307 84-85 0.5850243960312156 2307 86-87 0.48598421378542866 2307 88-89 0.6248279440806925 2307 90-91 0.5740843627081809 2307 92-93 0.5202934131515313 2307 94-95 0.4548804470859906 2307 96-97 0.48046618765268434 2307 98-99 0.5086732193248729 2307 100-101 0.25606502207904924 2201 1 0.1879470948818316 2201 2 0.08590427187444405 2201 3 0.14817369695957083 2201 4 0.29133521449656996 2201 5 0.2320892983317293 2201 6 0.3197042821260325 2201 7 0.48460789193949694 2201 8 0.21016947823398624 2201 9 0.3244672453789832 2201 10-11 0.30316359286204175 2201 12-13 0.28768703467224555 2201 14-15 0.41119512741903463 2201 16-17 0.3550722225727796 2201 18-19 0.3775239883875514 2201 20-21 0.2988827316546221 2201 22-23 0.2979456248365864 2201 24-25 0.30120978964830414 2201 26-27 0.35054418638083007 2201 28-29 0.34773479567021326 2201 30-31 0.3036961850722051 2201 32-33 0.3040147796601431 2201 34-35 0.2613002719808506 2201 36-37 0.31905640061759755 2201 38-39 0.34913652597123246 2201 40-41 0.23708914798816494 2201 42-43 0.21802372167017836 2201 44-45 0.2678620284403763 2201 46-47 0.23849730457313 2201 48-49 0.25773872173919443 2201 50-51 0.2150375660246837 2201 52-53 0.31437957476704526 2201 54-55 0.3894673640469861 2201 56-57 0.3216609453679027 2201 58-59 0.39114277224103944 2201 60-61 0.3505236511723169 2201 62-63 0.36288463367709767 2201 64-65 0.31857189083859083 2201 66-67 0.19993954860787966 2201 68-69 0.20740367672464544 2201 70-71 0.2398857093828184 2201 72-73 0.16574431832857783 2201 74-75 0.1485339146226181 2201 76-77 0.25519405890745617 2201 78-79 0.1979224636086272 2201 80-81 0.34000229607723753 2201 82-83 0.19479421582258283 2201 84-85 0.23591469756227923 2201 86-87 0.40373976029038516 2201 88-89 0.546051232756831 2201 90-91 0.3210445707718499 2201 92-93 0.29022097149623605 2201 94-95 0.26411074525774936 2201 96-97 0.23103713180450924 2201 98-99 0.3307066951163087 2201 100-101 0.26248238448898675 1101 1 0.28787694835544997 1101 2 0.15075089949899834 1101 3 0.2252418388736359 1101 4 0.266284856005619 1101 5 0.32649246529599196 1101 6 0.36665876030946265 1101 7 0.39362427007869627 1101 8 0.2657316145479385 1101 9 0.3434714471104172 1101 10-11 0.22846064088723494 1101 12-13 0.2612454341265149 1101 14-15 0.3987718496919683 1101 16-17 0.43657519858348337 1101 18-19 0.36052000599148926 1101 20-21 0.36158017074944837 1101 22-23 0.3958874181244596 1101 24-25 0.4261750576257626 1101 26-27 0.4081700833926831 1101 28-29 0.38770756858859556 1101 30-31 0.45516839921883445 1101 32-33 0.4256708762342285 1101 34-35 0.2804365377663629 1101 36-37 0.40256496952334686 1101 38-39 0.34470533961947325 1101 40-41 0.25613806401174344 1101 42-43 0.3388362870452255 1101 44-45 0.49190381292658714 1101 46-47 0.22488050825332095 1101 48-49 0.35878396146350156 1101 50-51 0.3725680176343289 1101 52-53 0.42324781524082056 1101 54-55 0.48839851988319793 1101 56-57 0.5309966306206988 1101 58-59 0.44128510489309036 1101 60-61 0.4913708865974584 1101 62-63 0.5490043838858014 1101 64-65 0.48980005594760456 1101 66-67 0.3894998674819732 1101 68-69 0.35831547174304035 1101 70-71 0.43947265914795963 1101 72-73 0.4032127065007245 1101 74-75 0.5798620969740895 1101 76-77 0.4030392379458334 1101 78-79 0.4111639030573997 1101 80-81 0.5156047348731789 1101 82-83 0.5636442160030128 1101 84-85 0.4886326989557972 1101 86-87 0.38975026642322774 1101 88-89 0.5573955624589928 1101 90-91 0.4978096988327998 1101 92-93 0.5544401556220002 1101 94-95 0.5856168606824426 1101 96-97 0.45164925163693326 1101 98-99 0.36610778467025185 1101 100-101 0.4364298061295422 2304 1 0.1467882728499461 2304 2 0.18839849273249598 2304 3 0.1409117102933024 2304 4 0.231601384146515 2304 5 0.24067002041864782 2304 6 0.24677618454051498 2304 7 0.32107808731672804 2304 8 0.28921846185341593 2304 9 0.3051104636524613 2304 10-11 0.28556662786210296 2304 12-13 0.28504295136844604 2304 14-15 0.3788394466675129 2304 16-17 0.38339986167419937 2304 18-19 0.3433188469574944 2304 20-21 0.3522718290865541 2304 22-23 0.36809896784215823 2304 24-25 0.4373479191810361 2304 26-27 0.5179588878687227 2304 28-29 0.44740797471074956 2304 30-31 0.4354401078434549 2304 32-33 0.4483675413100201 2304 34-35 0.37083398517009414 2304 36-37 0.4358536784471241 2304 38-39 0.27244706434289867 2304 40-41 0.3953056887024573 2304 42-43 0.4053459178132499 2304 44-45 0.33869900989932944 2304 46-47 0.34240854609002014 2304 48-49 0.43062350769529445 2304 50-51 0.44719533877899664 2304 52-53 0.47933774643318827 2304 54-55 0.5891874014013823 2304 56-57 0.5169241829918505 2304 58-59 0.6315776949398497 2304 60-61 0.5039900392739796 2304 62-63 0.47744148898228644 2304 64-65 0.571900965144934 2304 66-67 0.6582481227776142 2304 68-69 0.5604135193750963 2304 70-71 0.5781907879644237 2304 72-73 0.511500439890888 2304 74-75 0.596501198562656 2304 76-77 0.6829115781159061 2304 78-79 0.6177261426024288 2304 80-81 0.5715073135837301 2304 82-83 0.5653288875183264 2304 84-85 0.5922648857619777 2304 86-87 0.5302130995929701 2304 88-89 0.48512528061482385 2304 90-91 0.6422021815208332 2304 92-93 0.5839200311138946 2304 94-95 0.5613198202012946 2304 96-97 0.5063006459496187 2304 98-99 0.40361179936628844 2304 100-101 0.4573484986543548 2202 1 0.19040184085375955 2202 2 0.06538407006650715 2202 3 0.05945232569802883 2202 4 0.10110025538754286 2202 5 0.1582331000026329 2202 6 0.16977536886373912 2202 7 0.19830635653105588 2202 8 0.1520316068965215 2202 9 0.06797593731933205 2202 10-11 0.11439101679102492 2202 12-13 0.10003361304900693 2202 14-15 0.2093939096261579 2202 16-17 0.1949275712855254 2202 18-19 0.2136572774004577 2202 20-21 0.19016148492045915 2202 22-23 0.07611559310616656 2202 24-25 0.19955522407126836 2202 26-27 0.31489511076313903 2202 28-29 0.239163547825207 2202 30-31 0.21641851774172238 2202 32-33 0.20083758384975425 2202 34-35 0.13984507473868035 2202 36-37 0.2856486763257422 2202 38-39 0.210808257930573 2202 40-41 0.18614280358138302 2202 42-43 0.09330343068366176 2202 44-45 0.17546812541409196 2202 46-47 0.21364174572043027 2202 48-49 0.13461782928542476 2202 50-51 0.23641753740885463 2202 52-53 0.27232108906809316 2202 54-55 0.3250816852050704 2202 56-57 0.3260791201955655 2202 58-59 0.2202445449307433 2202 60-61 0.2971176433771845 2202 62-63 0.3117097178498227 2202 64-65 0.34518130462736707 2202 66-67 0.344024696184853 2202 68-69 0.3882262409160333 2202 70-71 0.2559007087057026 2202 72-73 0.2537465793495741 2202 74-75 0.481787224963675 2202 76-77 0.40467732688513536 2202 78-79 0.3725018546952157 2202 80-81 0.44223743518492853 2202 82-83 0.42272226843087424 2202 84-85 0.3365287519770632 2202 86-87 0.3196313855269324 2202 88-89 0.5635924000227135 2202 90-91 0.41598859642222763 2202 92-93 0.4611217105803824 2202 94-95 0.44768384332490285 2202 96-97 0.44339491057001723 2202 98-99 0.46733205123985044 2202 100-101 0.5041668564483217 1102 1 0.08688886236459936 1102 2 0.13307081027730305 1102 3 0.1333574040155021 1102 4 0.09874570281147754 1102 5 0.21712380054848524 1102 6 0.15049547724557755 1102 7 0.1774641212976249 1102 8 0.11056360898678719 1102 9 0.12693111649292632 1102 10-11 0.19833611516423844 1102 12-13 0.2414519046733261 1102 14-15 0.22583686191967445 1102 16-17 0.18796995443485542 1102 18-19 0.18889198080839265 1102 20-21 0.2823728095328235 1102 22-23 0.2419328801630911 1102 24-25 0.2654420662449084 1102 26-27 0.34230833167269026 1102 28-29 0.336437437156782 1102 30-31 0.2788796621507501 1102 32-33 0.2674954929714559 1102 34-35 0.28490625846042406 1102 36-37 0.28189324736964494 1102 38-39 0.29669312845364004 1102 40-41 0.33700156026021233 1102 42-43 0.28728533721081817 1102 44-45 0.415590595109137 1102 46-47 0.4105556554445755 1102 48-49 0.3475523923801731 1102 50-51 0.24229191932828087 1102 52-53 0.3736265166287609 1102 54-55 0.49640067733675863 1102 56-57 0.5114980905296207 1102 58-59 0.4844415117406058 1102 60-61 0.48441094486577896 1102 62-63 0.50011283967401 1102 64-65 0.5429977046219747 1102 66-67 0.5826360081901996 1102 68-69 0.5255778077466928 1102 70-71 0.5054620495339535 1102 72-73 0.5021684168172342 1102 74-75 0.6388245920125897 1102 76-77 0.5171383690426126 1102 78-79 0.5092776249742244 1102 80-81 0.6031806311980361 1102 82-83 0.5470242812707795 1102 84-85 0.5008954804277295 1102 86-87 0.324045795682224 1102 88-89 0.6202050562149708 1102 90-91 0.643178169121402 1102 92-93 0.50568005122771 1102 94-95 0.4622810648253477 1102 96-97 0.3633556712349737 1102 98-99 0.48383671535431816 1102 100-101 0.3949415046067344 2305 1 0.27045423599145835 2305 2 0.15734881614267593 2305 3 0.1858175317507076 2305 4 0.2673289281761697 2305 5 0.26555356048898204 2305 6 0.3332281888825648 2305 7 0.23559162210969475 2305 8 0.32721623742332895 2305 9 0.22048161674761957 2305 10-11 0.31457813978846616 2305 12-13 0.24433343308476196 2305 14-15 0.25586569739023446 2305 16-17 0.20658168305890712 2305 18-19 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14652.0 9 33317.0 10 46986.0 11 39637.0 12 27184.0 13 19387.0 14 15147.0 15 13244.0 16 12809.0 17 13260.0 18 14248.0 19 16148.0 20 17412.0 21 20227.0 22 22591.0 23 24474.0 24 26933.0 25 30785.0 26 35742.0 27 42364.0 28 50660.0 29 63328.0 30 78147.0 31 100665.0 32 135952.0 33 191687.0 34 290646.0 35 484623.0 36 933827.0 37 1552897.0 38 436532.0 39 379.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.892087304434064 24.117659427103945 26.329394517689902 14.660858750772086 2 29.596682775987464 22.960756895237516 34.88145307766569 12.56110725110933 3 38.567433785131506 19.483814397573653 26.622841432428885 15.325910384865956 4 28.21077527127326 18.475318071240718 36.76583718339835 16.548069474087672 5 32.92471006714147 17.548997123459806 33.691690332602306 15.834602476796423 6 25.270139323627745 18.37867289582695 40.924754751788164 15.426433028757142 7 32.82242524733759 18.762805386423832 31.829704147847632 16.585065218390945 8 27.75890541154323 27.948928304589245 28.020688838708992 16.271477445158535 9 31.722519510496433 25.46378773644958 25.193732011193315 17.619960741860677 10-11 25.34196783527387 28.67309012708601 28.855651852688474 17.12929018495165 12-13 26.46327257157017 25.182388277882232 28.82343358299522 19.530905567552374 14-15 24.367283194506122 25.718189344898473 30.204028303669944 19.710499156925458 16-17 23.674345505295378 26.88718049223794 31.38909416834358 18.049379834123105 18-19 22.939630694894365 27.305764485326478 31.441029275271053 18.3135755445081 20-21 22.65717276415132 28.20035639061252 30.647564301814228 18.494906543421934 22-23 23.80405769842477 27.228314608003405 30.323939351746954 18.64368834182487 24-25 22.5036844936459 28.36740396872825 30.2811605834231 18.847750954202752 26-27 22.140766235785897 28.801979664833173 29.932056048005435 19.1251980513755 28-29 22.01660049862082 28.602782170531903 29.662317588506546 19.718299742340722 30-31 22.503066151097304 28.147230636504965 29.75303910983603 19.5966641025617 32-33 22.44920064347622 27.767971539737445 30.506692740104302 19.276135076682028 34-35 21.53591720071256 28.03894807496135 30.21330418667332 20.211830537652776 36-37 20.65625791445062 28.50197777955506 30.369152887191586 20.472611418802735 38-39 21.06919070985225 28.713994594371627 30.464240730872287 19.75257396490383 40-41 21.54339365601892 28.018824419619804 30.71242311485227 19.725358809509004 42-43 21.992598785415524 28.17831189659849 30.820474435923888 19.008614882062094 44-45 21.10670366380646 28.940719885837634 30.92869633625604 19.02388011409986 46-47 21.786061415398365 28.928990008619294 30.08046620886037 19.204482367121972 48-49 21.125946399408548 28.895643471384542 30.159627535403484 19.81878259380343 50-51 20.800282329758158 28.449182727453838 30.321805493385213 20.42872944940279 52-53 22.31970253871236 28.05934931541459 29.515952371104397 20.104995774768657 54-55 22.925670198135936 28.293869434206982 29.164499708404584 19.6159606592525 56-57 23.065172792461595 28.273292412152816 28.78872673540861 19.87280805997698 58-59 22.38853583811217 28.155799732851282 29.205162235910386 20.250502193126167 60-61 21.081412160322678 28.468968887339162 29.054480640170905 21.39513831216726 62-63 21.946243091034763 28.47962884791957 29.203194643468134 20.370933417577536 64-65 21.21399905448381 28.89624521774988 29.73636967600451 20.153386051761803 66-67 21.164444120449684 28.39811087910772 29.276947830479 21.1604971699636 68-69 21.288967790958672 27.957452871218823 30.66510820498208 20.088471132840425 70-71 21.074323800892465 29.77347090413387 29.454852865105035 19.697352429868626 72-73 20.72712967193716 31.30247358124265 28.36071459661595 19.60968215020424 74-75 21.17929614386384 30.88369114465782 28.152516914362174 19.784495797116165 76-77 21.27367339832289 30.302241423261282 28.535754391460166 19.88833078695566 78-79 21.171102405022772 30.078946277751523 29.014329501261376 19.735621815964326 80-81 20.59911858118108 30.40401244076607 28.913261880792028 20.08360709726082 82-83 19.90559479932827 30.26615566368181 29.21141250185032 20.6168370351396 84-85 19.984835659927956 29.89998910434145 29.34123765097935 20.773937584751245 86-87 19.99896248249189 29.886254330541828 29.863451862416845 20.251331324549433 88-89 20.204600836602864 30.174712044077168 29.524177172812305 20.096509946507666 90-91 20.369666291045842 29.744264388740373 29.32679105370545 20.559278266508336 92-93 20.028212592336995 30.50099216781953 29.089008979592517 20.381786260250962 94-95 19.680299010424527 30.94862856541285 29.122985572522097 20.248086851640526 96-97 20.127292973221728 31.06599275452478 28.825965434325063 19.980748837928434 98-99 20.539290665280387 30.363498220498258 28.45390790578638 20.643303208434972 100-101 20.574713086660584 30.28327448425595 27.942762011372896 21.199250417710573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2643.0 1 1688.5 2 607.5 3 511.0 4 529.0 5 521.5 6 619.5 7 812.5 8 1071.0 9 1342.5 10 1563.0 11 1767.0 12 1813.5 13 1771.0 14 1698.5 15 1556.0 16 1480.5 17 1437.0 18 1523.0 19 1689.0 20 1915.0 21 2334.0 22 2957.5 23 3858.5 24 5170.0 25 7073.5 26 9991.5 27 14028.5 28 18715.5 29 23574.5 30 29136.5 31 35926.5 32 48337.5 33 69857.5 34 101748.5 35 140701.0 36 192194.0 37 254710.0 38 311591.5 39 335985.0 40 330870.0 41 307687.0 42 283296.0 43 284195.5 44 280148.5 45 261887.0 46 247759.0 47 220040.5 48 178297.0 49 148234.5 50 126214.0 51 107699.0 52 91664.5 53 74826.5 54 58326.5 55 43919.0 56 33374.5 57 25196.0 58 19427.5 59 15032.5 60 11277.5 61 8338.5 62 6165.5 63 4541.5 64 3326.5 65 2376.0 66 1694.0 67 1250.5 68 917.0 69 675.5 70 515.0 71 371.5 72 283.0 73 221.5 74 181.0 75 156.0 76 133.5 77 109.5 78 89.0 79 67.0 80 50.5 81 47.5 82 39.0 83 40.0 84 44.5 85 32.0 86 23.0 87 24.0 88 21.5 89 16.5 90 17.0 91 14.0 92 9.5 93 8.0 94 6.5 95 8.0 96 7.0 97 3.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26513969456820746 2 0.2506265664160401 3 0.2163888721057054 4 0.21937870108125918 5 0.23360115141635882 6 0.23671555659922736 7 0.21273463669113968 8 0.2089973504716974 9 0.19045625828302012 10-11 0.1793897385332273 12-13 0.20683802954490857 14-15 0.20377553111508787 16-17 0.2049901491364066 18-19 0.19704841592009184 20-21 0.19775434776154202 22-23 0.164305636097534 24-25 0.47607835760389294 26-27 0.33474665040531904 28-29 0.27573905354057 30-31 0.2799538818880521 32-33 0.3224447499329884 34-35 0.3181157267288011 36-37 0.3111290777685661 38-39 0.3352553365851876 40-41 0.34037334243570155 42-43 0.3750262907704187 44-45 0.34255342606370953 46-47 0.36943074279186494 48-49 0.3451695264173191 50-51 0.3671883710601996 52-53 0.35677587639880914 54-55 0.3324835159724346 56-57 0.3686313787949287 58-59 0.35565469053297644 60-61 0.32153119107934697 62-63 0.29931510077488477 64-65 0.30560619924427923 66-67 0.31463797427459794 68-69 0.3205968695244864 70-71 0.2977578981834505 72-73 0.30369603073211987 74-75 0.30243988730836285 76-77 0.3059799278662234 78-79 0.3080354352869167 80-81 0.32514390109147445 82-83 0.34232503635029915 84-85 0.33743542021319556 86-87 0.3407159270058171 88-89 0.33639728515223943 90-91 0.3102881883691916 92-93 0.29520408593349834 94-95 0.3036233612778529 96-97 0.29112421514394055 98-99 0.3111290777685661 100-101 0.3333555494236378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4816329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.898729099676146 #Duplication Level Percentage of deduplicated Percentage of total 1 91.74183953149428 47.61284876950981 2 4.032273303198508 4.185397196371113 3 1.2126155389662694 1.8879961607660458 4 0.6733123234365774 1.3977621549403383 5 0.3925063285250786 1.018528980701577 6 0.280625565193004 0.8738466111837133 7 0.1975487524792859 0.7176770432231006 8 0.1540939452111805 0.6397823934732315 9 0.12507478343919196 0.5842100072620154 >10 0.9189927238576667 9.431010154134741 >50 0.11970510118197118 4.4022754087876415 >100 0.12162174600868403 13.54660328940013 >500 0.022918801151119373 8.120292979568013 >1k 0.00675016097464873 5.1060797615139615 >5k 1.2139488254360299E-4 0.4756890891645273 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8559 0.17770795973447828 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7236 0.15023890602157783 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6856 0.14234907955831091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001453389085338647 0.0 0.0 2 0.001494914487776894 0.0 0.0 3 0.0016817787987490058 0.0 0.0 4 0.0017440669024063762 0.0 0.0 5 0.0019309312133784882 0.0 2.0762701219123527E-5 6 0.0020347447194741055 0.0 4.1525402438247054E-5 7 0.0023046598353227117 0.0 4.1525402438247054E-5 8 0.002553812249952194 0.0 4.1525402438247054E-5 9 0.003072879780430282 0.0 4.1525402438247054E-5 10-11 0.004733895877960165 0.0 5.190675304780882E-5 12-13 0.00676864059743427 0.0 6.228810365737058E-5 14-15 0.008803385316908375 0.0 6.228810365737058E-5 16-17 0.010817367335163357 0.0 6.228810365737058E-5 18-19 0.01372414550584065 0.0 6.228810365737058E-5 20-21 0.01698388959724305 0.0 6.228810365737058E-5 22-23 0.020627743661199224 0.0 6.228810365737058E-5 24-25 0.024489606087956202 0.0 6.228810365737058E-5 26-27 0.029296171420183297 0.0 8.305080487649411E-5 28-29 0.034559516179231115 0.0 1.0381350609561763E-4 30-31 0.054626666907514 0.0 1.0381350609561763E-4 32-33 0.06094890942873711 0.0 1.0381350609561763E-4 34-35 0.06734382140422716 0.0 1.0381350609561763E-4 36-37 0.0740916993004423 0.0 1.0381350609561763E-4 38-39 0.08236563573626304 0.0 1.0381350609561763E-4 40-41 0.090847199184275 0.0 1.0381350609561763E-4 42-43 0.10036689769324314 0.0 1.0381350609561763E-4 44-45 0.11108045152231087 0.0 1.141948567051794E-4 46-47 0.12273870825684874 0.0 1.2457620731474116E-4 48-49 0.13563234571392446 0.0 1.453389085338647E-4 50-51 0.1489516185459922 0.0 1.453389085338647E-4 52-53 0.1630910180762153 0.0 1.453389085338647E-4 54-55 0.17855923048446232 0.0 1.453389085338647E-4 56-57 0.19504481525244644 2.0762701219123527E-5 1.453389085338647E-4 58-59 0.212703492639311 2.0762701219123527E-5 1.453389085338647E-4 60-61 0.23088123755665363 2.0762701219123527E-5 1.453389085338647E-4 62-63 0.2521007182025979 2.0762701219123527E-5 1.5572025914342645E-4 64-65 0.2761958329673907 2.0762701219123527E-5 1.6610160975298821E-4 66-67 0.3014744217016736 2.0762701219123527E-5 1.6610160975298821E-4 68-69 0.3287047043505541 2.0762701219123527E-5 1.6610160975298821E-4 70-71 0.3595892224140004 2.0762701219123527E-5 1.8686431097211174E-4 72-73 0.3918544601085183 2.0762701219123527E-5 1.8686431097211174E-4 74-75 0.42675656085786495 2.0762701219123527E-5 1.8686431097211174E-4 76-77 0.4641501857535064 2.0762701219123527E-5 1.972456615816735E-4 78-79 0.5081359682862197 2.0762701219123527E-5 2.0762701219123527E-4 80-81 0.5544679360566938 2.0762701219123527E-5 2.0762701219123527E-4 82-83 0.6052327405374508 2.0762701219123527E-5 2.0762701219123527E-4 84-85 0.661468516789447 2.0762701219123527E-5 2.0762701219123527E-4 86-87 0.7252100095321561 2.0762701219123527E-5 2.0762701219123527E-4 88-89 0.7934051016863674 2.0762701219123527E-5 2.0762701219123527E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1500 0.0 78.42335 9 CGGGAGA 2100 0.0 69.13964 4 AGAGCGG 1815 0.0 68.99701 8 AAGCGGC 680 0.0 64.17469 9 ATTCGGA 635 0.0 62.773422 2 CGGAAAG 1200 0.0 58.12112 5 GAGAGCG 2360 0.0 57.69053 7 TCGGGAG 2530 0.0 57.20169 3 GATCCGG 555 0.0 52.161766 1 TTGTGTT 65620 0.0 50.879555 1 TCGGGAA 910 0.0 50.056374 3 GATCGGG 3965 0.0 47.279118 1 TCGGAAA 1225 0.0 46.86777 4 AAGCGTC 465 0.0 44.88328 9 ATCGGGA 4045 0.0 44.227566 2 GGATCGG 1160 0.0 43.367393 1 AAAGCGG 1070 0.0 43.002224 8 AAGGGGC 2190 0.0 42.451874 9 GGTTCGG 620 0.0 42.10042 1 GGCGCCG 1875 0.0 42.055916 56-57 >>END_MODULE