FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4816329
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC85590.17770795973447828No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG72360.15023890602157783No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA68560.14234907955831091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC15000.078.423359
CGGGAGA21000.069.139644
AGAGCGG18150.068.997018
AAGCGGC6800.064.174699
ATTCGGA6350.062.7734222
CGGAAAG12000.058.121125
GAGAGCG23600.057.690537
TCGGGAG25300.057.201693
GATCCGG5550.052.1617661
TTGTGTT656200.050.8795551
TCGGGAA9100.050.0563743
GATCGGG39650.047.2791181
TCGGAAA12250.046.867774
AAGCGTC4650.044.883289
ATCGGGA40450.044.2275662
GGATCGG11600.043.3673931
AAAGCGG10700.043.0022248
AAGGGGC21900.042.4518749
GGTTCGG6200.042.100421
GGCGCCG18750.042.05591656-57
Produced by FastQC (version 0.11.2)