FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664865
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60550.2272160128186606No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG57300.21502027307199428No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC57080.2141947153045276No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT52930.1986216937818614No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52770.1980212881327947No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT47550.17843305383199523No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA40990.15381642222026257No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG40270.15111459679946262No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA39470.14811256855412938No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT39110.1467616558437294No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG33370.12522210318346333No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA29790.11178802678559702No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT28930.10856084642186378No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA28390.10653447735626381No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28100.10544624211733052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4100.056.6976979
CGGGAGA5400.056.2463874
ATTCGGA851.8189894E-1255.8401642
TTGTGTT359400.049.372381
CGACGCC502.8384464E-547.4703871
CGAGGCG1200.047.4703871
AAGCGGC1950.046.2244959
GTTCGGA1052.0008883E-1145.2039382
AGAGCGG4950.045.0457738
TCGGGAA2300.043.331933
GGAGAGC5950.041.470346
ATATGCG704.330852E-640.6889081
ATCGGGA9000.040.6082082
GAACCGG951.9404979E-839.9750671
CGGAAAG2150.039.7321855
GACGCGT608.351021E-539.5370867
TGTGTTT620000.038.650081
GGTTCGG2600.038.341471
AACCGGA1251.364242E-1037.9713132
TCGGAGA1750.037.966314
Produced by FastQC (version 0.11.2)