##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34310-1.34200000035810.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5767855 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926822016156787 33.0 33.0 33.0 27.0 33.0 2 31.91263060531168 33.0 33.0 33.0 33.0 33.0 3 31.875492709161378 33.0 33.0 33.0 33.0 33.0 4 35.58861465830885 37.0 37.0 37.0 33.0 37.0 5 35.546607014219326 37.0 37.0 37.0 33.0 37.0 6 35.54820968973735 37.0 37.0 37.0 33.0 37.0 7 35.52585129133794 37.0 37.0 37.0 33.0 37.0 8 35.491159885260636 37.0 37.0 37.0 33.0 37.0 9 35.381918234768385 37.0 37.0 37.0 33.0 37.0 10-11 35.299881671782664 37.0 37.0 37.0 33.0 37.0 12-13 35.30576210740388 37.0 37.0 37.0 33.0 37.0 14-15 36.98113215398099 40.0 37.0 40.0 33.0 40.0 16-17 36.90085855140256 40.0 37.0 40.0 33.0 40.0 18-19 36.944493143464946 40.0 37.0 40.0 33.0 40.0 20-21 36.92619370632583 40.0 37.0 40.0 33.0 40.0 22-23 36.94684982545505 40.0 37.0 40.0 33.0 40.0 24-25 36.74541046888315 40.0 37.0 40.0 33.0 40.0 26-27 36.62539505587432 40.0 37.0 40.0 33.0 40.0 28-29 36.56833866315988 40.0 37.0 40.0 33.0 40.0 30-31 36.618985047300946 40.0 37.0 40.0 33.0 40.0 32-33 36.51701065647455 40.0 37.0 40.0 33.0 40.0 34-35 36.44310458567353 40.0 37.0 40.0 33.0 40.0 36-37 36.316529888494074 38.5 37.0 40.0 33.0 40.0 38-39 36.23767058984666 37.0 37.0 40.0 33.0 40.0 40-41 36.17997539813327 37.0 37.0 40.0 33.0 40.0 42-43 36.01726751105913 37.0 37.0 40.0 33.0 40.0 44-45 35.886956156144706 37.0 37.0 40.0 27.0 40.0 46-47 35.78901966155529 37.0 37.0 40.0 27.0 40.0 48-49 35.648094915700895 37.0 37.0 40.0 27.0 40.0 50-51 34.445834369969425 37.0 35.0 38.5 27.0 38.5 52-53 34.898882340142045 37.0 35.0 38.5 27.0 40.0 54-55 35.66386637666862 37.0 37.0 40.0 27.0 40.0 56-57 35.66978634171629 37.0 37.0 40.0 27.0 40.0 58-59 35.467575467829896 37.0 37.0 40.0 27.0 40.0 60-61 35.20309291755774 37.0 35.0 40.0 27.0 40.0 62-63 34.984079956933726 37.0 33.0 40.0 27.0 40.0 64-65 34.718486855165395 37.0 33.0 37.0 27.0 40.0 66-67 34.44081699349238 37.0 33.0 37.0 27.0 40.0 68-69 34.18585843437465 37.0 33.0 37.0 27.0 40.0 70-71 33.88710482493059 37.0 33.0 37.0 27.0 37.0 72-73 33.62987167673251 37.0 33.0 37.0 27.0 37.0 74-75 33.37802831381857 37.0 33.0 37.0 27.0 37.0 76-77 33.13435081152352 37.0 33.0 37.0 27.0 37.0 78-79 32.9412721020206 37.0 33.0 37.0 27.0 37.0 80-81 32.78022869853697 37.0 33.0 37.0 27.0 37.0 82-83 32.54294846177652 35.0 33.0 37.0 22.0 37.0 84-85 32.40613919732725 33.0 33.0 37.0 22.0 37.0 86-87 32.24546794258871 33.0 33.0 37.0 22.0 37.0 88-89 32.06625183885517 33.0 33.0 37.0 22.0 37.0 90-91 32.01678500933189 33.0 33.0 37.0 22.0 37.0 92-93 31.829396023305023 33.0 33.0 37.0 22.0 37.0 94-95 31.651497220370484 33.0 33.0 37.0 22.0 37.0 96-97 31.501609783879797 33.0 33.0 37.0 22.0 37.0 98-99 31.35428517187065 33.0 33.0 37.0 18.5 37.0 100-101 30.033075467396458 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.27249677342812717 2306 2 0.241431682903535 2306 3 0.20658190764999418 2306 4 0.24915138174207385 2306 5 0.3125157888238945 2306 6 0.3295730144304372 2306 7 0.3355608400762833 2306 8 0.23422479561669718 2306 9 0.31469587374026275 2306 10-11 0.3415202949082712 2306 12-13 0.30479922657993086 2306 14-15 0.4943199811202277 2306 16-17 0.4436626960863066 2306 18-19 0.4451952316293202 2306 20-21 0.48385301194718977 2306 22-23 0.41750082141064127 2306 24-25 0.4084892971634275 2306 26-27 0.44269371230807764 2306 28-29 0.3328279334427222 2306 30-31 0.2397412695307537 2306 32-33 0.22321607339593896 2306 34-35 0.14206153346845696 2306 36-37 0.23015534200187204 2306 38-39 0.31953148770596584 2306 40-41 0.24275985896213825 2306 42-43 0.3546701792826852 2306 44-45 0.32936300007386166 2306 46-47 0.3163880583504337 2306 48-49 0.3990959737905442 2306 50-51 0.3965392991918506 2306 52-53 0.4136294636459539 2306 54-55 0.404701274662429 2306 56-57 0.4239192516182726 2306 58-59 0.3869137682078403 2306 60-61 0.3066943787551324 2306 62-63 0.28881712604446363 2306 64-65 0.34960212021252346 2306 66-67 0.33278772379031096 2306 68-69 0.2967989670124851 2306 70-71 0.30284680969652555 2306 72-73 0.22458197409348202 2306 74-75 0.3466363834855102 2306 76-77 0.2668297702051774 2306 78-79 0.2748398067712188 2306 80-81 0.11230725942142783 2306 82-83 0.2739732293229906 2306 84-85 0.17664027471396082 2306 86-87 0.2513418984932869 2306 88-89 0.31593776495589765 2306 90-91 0.20439954567856944 2306 92-93 0.22938686150264687 2306 94-95 0.14332758435976345 2306 96-97 0.11076331793296745 2306 98-99 0.09263468002514585 2306 100-101 -0.011734699092436074 2307 1 0.317673224128729 2307 2 0.3223948852029075 2307 3 0.29144023213295256 2307 4 0.3806405392082013 2307 5 0.3740653370593847 2307 6 0.42356814600603343 2307 7 0.3975838714816575 2307 8 0.4027234099721966 2307 9 0.47921135454092934 2307 10-11 0.5081708955605606 2307 12-13 0.34136716350841567 2307 14-15 0.49484062127802986 2307 16-17 0.5268502394835366 2307 18-19 0.4871844776976957 2307 20-21 0.40950571558729365 2307 22-23 0.40393758819474357 2307 24-25 0.5705450177027913 2307 26-27 0.6007181293860882 2307 28-29 0.6256850678588748 2307 30-31 0.5804250154680375 2307 32-33 0.5373876036193721 2307 34-35 0.6330825165836131 2307 36-37 0.6357760047758916 2307 38-39 0.42464089670116323 2307 40-41 0.4998369382969017 2307 42-43 0.5438954715673745 2307 44-45 0.5560423642378538 2307 46-47 0.40317979120148806 2307 48-49 0.32916705647840416 2307 50-51 0.366670158707862 2307 52-53 0.49530196219143363 2307 54-55 0.42706902139856595 2307 56-57 0.49381744373302183 2307 58-59 0.4699301564991458 2307 60-61 0.4428721957140951 2307 62-63 0.47387856321500976 2307 64-65 0.3792184812338135 2307 66-67 0.4965846232577462 2307 68-69 0.44668773448245247 2307 70-71 0.4009816551597538 2307 72-73 0.3628400726168124 2307 74-75 0.4617047518329116 2307 76-77 0.4112203592685546 2307 78-79 0.4497075407051909 2307 80-81 0.3046933360450126 2307 82-83 0.3572479399926465 2307 84-85 0.4353569878876584 2307 86-87 0.48353730782240945 2307 88-89 0.5126365675904552 2307 90-91 0.33107086839437727 2307 92-93 0.39249036191858977 2307 94-95 0.3574180304496757 2307 96-97 0.27153758390322125 2307 98-99 0.3035437457647454 2307 100-101 0.2512543921682102 2201 1 0.4092936681624799 2201 2 0.3352178526358678 2201 3 0.3279094955059172 2201 4 0.454087284758792 2201 5 0.46973249478057966 2201 6 0.4763000990501709 2201 7 0.47114312110291223 2201 8 0.4607059170715786 2201 9 0.5144438533312368 2201 10-11 0.5053464348350118 2201 12-13 0.5287789722539955 2201 14-15 0.7206492423354547 2201 16-17 0.6752246180779906 2201 18-19 0.6364016225690747 2201 20-21 0.5737157052934094 2201 22-23 0.5816807565371889 2201 24-25 0.6571637117599067 2201 26-27 0.7213379409940401 2201 28-29 0.6295757426133548 2201 30-31 0.49211579561121255 2201 32-33 0.6037878650157964 2201 34-35 0.5224474022375034 2201 36-37 0.5516052494990831 2201 38-39 0.5892777309083925 2201 40-41 0.5008589152883403 2201 42-43 0.5906463742916159 2201 44-45 0.5615982084654263 2201 46-47 0.4547225715375234 2201 48-49 0.5683415824360267 2201 50-51 0.4569237797083261 2201 52-53 0.47452491015072695 2201 54-55 0.6659967717837034 2201 56-57 0.5920497254731742 2201 58-59 0.6358452616452013 2201 60-61 0.6248816245226649 2201 62-63 0.6669654299329579 2201 64-65 0.6010071492808038 2201 66-67 0.5693856016842744 2201 68-69 0.6100821086859725 2201 70-71 0.5119730844092842 2201 72-73 0.49945683959310117 2201 74-75 0.5850047661728865 2201 76-77 0.4107694067522871 2201 78-79 0.4316696741131736 2201 80-81 0.6541355826810857 2201 82-83 0.5351874053127545 2201 84-85 0.549021832175626 2201 86-87 0.6918549007283801 2201 88-89 0.688799685697731 2201 90-91 0.5842621376415877 2201 92-93 0.5599345705881973 2201 94-95 0.4576266232952584 2201 96-97 0.4779050802444935 2201 98-99 0.5060197091374157 2201 100-101 0.34929700858534574 1101 1 0.2349488514775082 1101 2 0.2027564958687229 1101 3 0.20318173084919877 1101 4 0.2847001695199296 1101 5 0.3443926191383966 1101 6 0.37521972363408906 1101 7 0.28849864925687285 1101 8 0.3293965158864012 1101 9 0.3233823585149409 1101 10-11 0.3033267146767855 1101 12-13 0.32203167200938765 1101 14-15 0.38228170551425933 1101 16-17 0.37607458817996076 1101 18-19 0.3335575355285414 1101 20-21 0.3702364833000118 1101 22-23 0.39708135345662043 1101 24-25 0.37711242959941416 1101 26-27 0.3930973767388295 1101 28-29 0.373997967627794 1101 30-31 0.4646211558416553 1101 32-33 0.5011093943656775 1101 34-35 0.41249184346379764 1101 36-37 0.44795797384019664 1101 38-39 0.3709207666551748 1101 40-41 0.3598055959918227 1101 42-43 0.46000935340842375 1101 44-45 0.4585456279235487 1101 46-47 0.3004895808188053 1101 48-49 0.3554180615248086 1101 50-51 0.39314705871409217 1101 52-53 0.4570424307273626 1101 54-55 0.40313339004325144 1101 56-57 0.49878514695967624 1101 58-59 0.4996168004720616 1101 60-61 0.5613504530759812 1101 62-63 0.5653141405789484 1101 64-65 0.5193697216329767 1101 66-67 0.47487363525525694 1101 68-69 0.3789542726620425 1101 70-71 0.42298064682422876 1101 72-73 0.40096411675221333 1101 74-75 0.5227855822625003 1101 76-77 0.5338622330945455 1101 78-79 0.3995831371237344 1101 80-81 0.4918512075782999 1101 82-83 0.5136453721379013 1101 84-85 0.528510955658156 1101 86-87 0.5279409587814357 1101 88-89 0.7034558488919913 1101 90-91 0.4849555542303392 1101 92-93 0.47778926687415435 1101 94-95 0.4582131310610542 1101 96-97 0.4730655274821025 1101 98-99 0.3914780505457003 1101 100-101 0.5426042039182732 2304 1 0.18718931280766427 2304 2 0.22270007031431405 2304 3 0.2194542997223614 2304 4 0.2608851331346145 2304 5 0.30326324494418344 2304 6 0.27819668409843956 2304 7 0.2640246314445207 2304 8 0.22869125040038796 2304 9 0.1971746844237643 2304 10-11 0.2751692841433311 2304 12-13 0.2427229120229768 2304 14-15 0.30463960320383876 2304 16-17 0.30394762528812436 2304 18-19 0.3173594095474357 2304 20-21 0.3092956977656627 2304 22-23 0.2824384365899064 2304 24-25 0.37133296396991256 2304 26-27 0.3733457354888401 2304 28-29 0.3358273207837996 2304 30-31 0.24623958747017127 2304 32-33 0.29658035354615464 2304 34-35 0.3648839956725354 2304 36-37 0.3868216947980301 2304 38-39 0.29785463655273503 2304 40-41 0.22657299432062672 2304 42-43 0.23247864571545307 2304 44-45 0.27846212241854573 2304 46-47 0.22917663476832217 2304 48-49 0.2564781714629305 2304 50-51 0.3223944842307418 2304 52-53 0.35072330807461327 2304 54-55 0.40803184022854566 2304 56-57 0.33395164550049117 2304 58-59 0.437060280515432 2304 60-61 0.312856626981052 2304 62-63 0.2773512325590701 2304 64-65 0.28291978447787614 2304 66-67 0.41235671028614007 2304 68-69 0.4147349790064183 2304 70-71 0.3498990887021307 2304 72-73 0.3703271984721752 2304 74-75 0.39188487721278875 2304 76-77 0.4071505051024005 2304 78-79 0.32475497613288695 2304 80-81 0.24325003267526313 2304 82-83 0.3471403580315098 2304 84-85 0.4105118511306287 2304 86-87 0.32282662420918484 2304 88-89 0.2600928642714564 2304 90-91 0.39990703149020135 2304 92-93 0.39024507315001244 2304 94-95 0.32705176800090996 2304 96-97 0.3274717375297378 2304 98-99 0.26695084382642165 2304 100-101 0.2545671766710953 2202 1 0.24466503669272655 2202 2 0.2847625154593878 2202 3 0.2591406945018697 2202 4 0.36379374858136515 2202 5 0.4416922999100663 2202 6 0.36892899607225615 2202 7 0.32726804435539236 2202 8 0.37938121787229306 2202 9 0.37050304624333563 2202 10-11 0.2971732506915714 2202 12-13 0.30185043135090694 2202 14-15 0.4562782834468635 2202 16-17 0.4145918694947426 2202 18-19 0.4039687905139999 2202 20-21 0.3215968555419195 2202 22-23 0.3431393000738794 2202 24-25 0.3792924907831008 2202 26-27 0.4212949860631312 2202 28-29 0.4585091992162873 2202 30-31 0.39809406873003184 2202 32-33 0.3722089134932176 2202 34-35 0.39137060157477777 2202 36-37 0.41269590483715035 2202 38-39 0.3379954850022031 2202 40-41 0.3000333878166046 2202 42-43 0.23841925145375598 2202 44-45 0.3209391958096859 2202 46-47 0.25063891957208995 2202 48-49 0.2557657722010589 2202 50-51 0.3124847112076665 2202 52-53 0.37174857282771256 2202 54-55 0.47192127669480044 2202 56-57 0.3484550572517833 2202 58-59 0.3375537199166132 2202 60-61 0.3055058373700419 2202 62-63 0.35802730511652214 2202 64-65 0.42247854597116685 2202 66-67 0.4219133125696999 2202 68-69 0.48730495839396326 2202 70-71 0.4964153837381531 2202 72-73 0.4992183643054773 2202 74-75 0.5628493442178666 2202 76-77 0.5072048052285538 2202 78-79 0.5772231327875588 2202 80-81 0.4222523378315799 2202 82-83 0.49922409538363866 2202 84-85 0.5026262972433457 2202 86-87 0.4380476932978965 2202 88-89 0.7166317710315866 2202 90-91 0.6247732504540551 2202 92-93 0.5033327230701445 2202 94-95 0.49777308399925957 2202 96-97 0.4932345883263416 2202 98-99 0.5145601588171331 2202 100-101 0.47228731110189415 1102 1 0.11078067614603881 1102 2 0.1823456163918813 1102 3 0.11693867804656932 1102 4 0.21198308352069262 1102 5 0.19023222108064175 1102 6 0.1745264398409887 1102 7 0.21497864388795307 1102 8 0.11562403026447754 1102 9 0.1389562533973958 1102 10-11 0.19036394018728942 1102 12-13 0.2636171717370175 1102 14-15 0.3519280884572211 1102 16-17 0.20894188298055383 1102 18-19 0.2593970286413523 1102 20-21 0.2129241430298734 1102 22-23 0.20321026433801137 1102 24-25 0.24489730525070286 1102 26-27 0.25752228473262306 1102 28-29 0.1973342024075393 1102 30-31 0.18120081606406302 1102 32-33 0.15130029932876 1102 34-35 0.12391867189399619 1102 36-37 0.16323595343386899 1102 38-39 0.2255565679511662 1102 40-41 0.15199662066728337 1102 42-43 0.13663263004901438 1102 44-45 0.1950647749507013 1102 46-47 0.09321509908370729 1102 48-49 0.19828442321377793 1102 50-51 0.11664476412612146 1102 52-53 0.1454782756760622 1102 54-55 0.2081770296380583 1102 56-57 0.2504210649450158 1102 58-59 0.35750724671908785 1102 60-61 0.3629584482504029 1102 62-63 0.3479051799262294 1102 64-65 0.3843068333361046 1102 66-67 0.3646768817178412 1102 68-69 0.37662072778310574 1102 70-71 0.35497351728717774 1102 72-73 0.37287098777905925 1102 74-75 0.39273745345359146 1102 76-77 0.40011795444927856 1102 78-79 0.28857310526694135 1102 80-81 0.4572832312031494 1102 82-83 0.3496080581530947 1102 84-85 0.3494937539571126 1102 86-87 0.21443403712874698 1102 88-89 0.3388991766703242 1102 90-91 0.42373911929311703 1102 92-93 0.2939072887661993 1102 94-95 0.3783751942091378 1102 96-97 0.3012812507957676 1102 98-99 0.43757876251187966 1102 100-101 0.46804876711219734 2305 1 0.2736299116527583 2305 2 0.29905932591148954 2305 3 0.30776884410093075 2305 4 0.36425983034880005 2305 5 0.2903199646757102 2305 6 0.37024004265933286 2305 7 0.3104978705083923 2305 8 0.3148441154249966 2305 9 0.3778026410652018 2305 10-11 0.39782295598415374 2305 12-13 0.42806622243220005 2305 14-15 0.40621385051230163 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16317.0 20 18847.0 21 21582.0 22 24415.0 23 26683.0 24 30399.0 25 35522.0 26 42127.0 27 50675.0 28 61464.0 29 76776.0 30 98212.0 31 130583.0 32 180704.0 33 258921.0 34 392072.0 35 667135.0 36 1276638.0 37 1827616.0 38 329155.0 39 92.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.06038476828917 23.831954649539895 24.91799950249526 15.189661079675679 2 32.11463563515823 21.552529050325163 33.524673136044655 12.80816217847196 3 40.65889445839234 18.769379121022318 24.671056632294047 15.900669788291303 4 31.471742357690125 17.515039976870128 34.33109285865202 16.682124806787726 5 33.977570616412706 16.379063012370306 32.83521345450217 16.808152916714818 6 26.506157469389912 17.092973060897638 39.391086197620155 17.009783272092303 7 34.45701421143871 17.13442786093854 29.819489466773085 18.589068460849656 8 29.944925075273925 25.768379547034325 26.052814318055823 18.233881059635923 9 32.01622070046524 24.842229265906653 24.25616909104047 18.88538094258764 10-11 26.862689435960903 27.682605641563285 28.005959237612878 17.448745684862935 12-13 27.333845657918843 25.102610414504294 27.756066090740173 19.807477836836693 14-15 26.419474142018036 25.6999108276559 27.776073355849075 20.10454167447699 16-17 25.045960884106304 27.03484132517791 28.951747868644073 18.967449922071708 18-19 24.09718178621904 27.26317318128315 29.071050477565603 19.568594554932204 20-21 23.742671204136915 28.08785687043968 28.709528279296055 19.45994364612735 22-23 24.280861132846894 27.528473293816468 28.46727668091677 19.72338889241987 24-25 24.29398110245657 27.13260157203147 28.921386581643283 19.652030743868682 26-27 23.172540603178643 27.195891339168465 29.325069209543564 20.306498848109328 28-29 22.511433482047703 27.822426279988445 28.394734948016037 21.271405289947822 30-31 21.698110419251 28.70423284516773 28.486179555809006 21.111477179772262 32-33 21.517502952974866 27.58959502282631 29.48516050772905 21.40774151646978 34-35 21.78990614309391 27.793170428647933 29.299467414279896 21.117456013978266 36-37 21.24267469088309 29.17533054326941 29.487282275534582 20.094712490312922 38-39 21.83022368424486 28.502393230935695 28.043039016231177 21.624344068588268 40-41 22.21509344287221 27.816429369936905 27.625831170634097 22.342646016556785 42-43 23.177050159443503 28.082739727362398 27.99314194544046 20.74706816775364 44-45 21.68652260509843 28.918132538834833 28.044789617866368 21.350555238200368 46-47 22.011571400181882 28.646585048294792 28.68110991890484 20.660733632618484 48-49 22.352859408017274 28.135480932466567 28.704302308062474 20.807357351453685 50-51 21.31676939517125 28.54936249510806 27.673198936224452 22.460669173496235 52-53 21.760045336019576 28.696346714761077 27.87724997788094 21.6663579713384 54-55 22.27405758517718 28.577616360155545 27.966351053408285 21.18197500125899 56-57 22.729900353789898 28.362600626349593 27.086392009509574 21.821107010350936 58-59 21.91794991627019 27.686985779807777 27.91819646444352 22.476867839478523 60-61 20.917466745598336 27.71857467630927 28.65905823106612 22.704900347026275 62-63 21.358034910334855 27.812828116908477 28.5670340126922 22.262102960064468 64-65 21.743453891709887 28.515409689300224 28.21368260171181 21.527453817278076 66-67 22.0962149466717 28.010095539429408 28.029140117307644 21.864549396591247 68-69 21.593478083342966 26.961330800499507 29.451915944773372 21.99327517138416 70-71 21.27969546602985 28.781510248729347 28.916241942160493 21.02255234308031 72-73 22.3820127097348 29.397557109810062 27.882619815040698 20.33781036541444 74-75 23.35578648955254 28.539497965060445 27.56714231047438 20.53757323491264 76-77 23.374645119496364 27.211380523201466 27.96742707830631 21.446547278995855 78-79 23.24443059330657 27.325801313349896 28.712611710306156 20.71715638303737 80-81 22.28599577201513 28.667518571902313 28.151392019706822 20.895093636375734 82-83 21.477981337911288 28.624565162477356 28.551750101424396 21.34570339818696 84-85 21.621832021601904 27.883176518192425 28.98317300416013 21.511818456045546 86-87 20.807004127023173 28.46546516820625 29.466959372229027 21.26057133254155 88-89 20.766301360736104 29.114514741706465 29.285107975261937 20.83407592229549 90-91 21.510372440911674 28.09820394793193 28.65818923481808 21.733234376338324 92-93 21.415446674373346 28.560895977920502 27.759067519687502 22.264589828018647 94-95 21.149499566197377 29.700863135924298 27.25072609116272 21.898911206715603 96-97 20.98075188613154 30.550470982986255 26.55689206349372 21.911885067388482 98-99 21.378033260592908 29.0800749056831 27.387001246543964 22.15489058718003 100-101 22.044606470846816 29.053229291461914 27.13188624048744 21.77027799720383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3156.0 1 1996.0 2 693.5 3 507.0 4 474.0 5 481.0 6 519.5 7 653.0 8 891.5 9 1241.5 10 1582.0 11 1827.5 12 2020.5 13 1988.0 14 1746.5 15 1557.5 16 1389.5 17 1230.5 18 1117.0 19 1115.0 20 1199.5 21 1314.5 22 1504.5 23 1837.0 24 2387.5 25 3055.0 26 3765.5 27 4751.5 28 6100.0 29 7550.0 30 9420.5 31 12632.5 32 18976.0 33 30651.5 34 49697.0 35 82532.0 36 126591.5 37 189070.5 38 272512.5 39 327942.5 40 347064.5 41 337028.5 42 346739.5 43 391159.0 44 388688.5 45 370450.5 46 386952.0 47 373200.5 48 309318.5 49 251468.5 50 202327.0 51 167202.0 52 150479.0 53 138623.0 54 113134.0 55 72441.5 56 47413.5 57 38584.5 58 33778.5 59 28880.0 60 22852.5 61 17675.0 62 12997.0 63 9294.5 64 7007.0 65 5296.5 66 4295.0 67 3624.5 68 2868.5 69 2122.5 70 1598.5 71 1236.0 72 974.0 73 737.5 74 524.5 75 382.0 76 288.0 77 214.0 78 157.5 79 124.0 80 100.0 81 80.0 82 61.0 83 54.0 84 51.0 85 35.5 86 27.0 87 27.5 88 18.5 89 17.5 90 19.5 91 11.0 92 10.5 93 10.5 94 9.5 95 8.5 96 3.5 97 3.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26259328641236646 2 0.24950349826755353 3 0.2133375405588386 4 0.21718645839744585 5 0.231455194348679 6 0.23480132562278352 7 0.20924589817185069 8 0.20593444183322918 9 0.18667251517245145 10-11 0.17461430635825623 12-13 0.20351586508329353 14-15 0.20087190125271873 16-17 0.20114063200271157 18-19 0.1934688025271093 20-21 0.19411895756741457 22-23 0.16125578746345046 24-25 0.4681636414230247 26-27 0.3264385113703448 28-29 0.27547502494428167 30-31 0.278266357250659 32-33 0.3219221010236908 34-35 0.3182812327979812 36-37 0.3108087842014059 38-39 0.33548000079752355 40-41 0.3404558540393266 42-43 0.3748187151029282 44-45 0.3437413041763359 46-47 0.36881995126437817 48-49 0.3453103450069393 50-51 0.36675679260314276 52-53 0.3562589558856802 54-55 0.3329747367088805 56-57 0.36797041534504593 58-59 0.35540941996634795 60-61 0.32210414443497626 62-63 0.29884593145978877 64-65 0.30558153767735147 66-67 0.3153165258141891 68-69 0.32184408241885415 70-71 0.29762363998401486 72-73 0.3054341692015489 74-75 0.3023394312096958 76-77 0.3065264296692618 78-79 0.309057699959517 80-81 0.325606312918754 82-83 0.34208557600702516 84-85 0.3376125093297248 86-87 0.3405772163135169 88-89 0.33609548090234587 90-91 0.3100372668869103 92-93 0.29471961413731795 94-95 0.3041685340564213 96-97 0.29123478312128165 98-99 0.31133757696752085 100-101 0.33412767831368856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5767855.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.704392641309436 #Duplication Level Percentage of deduplicated Percentage of total 1 83.58058726796112 26.49871755934668 2 7.146126108531632 4.531271760183991 3 2.312793770982401 2.1997716544080217 4 1.3493473388532444 1.7112095136203713 5 0.9486291761293966 1.5037855935504139 6 0.6043790651629117 1.1496882711667498 7 0.5184734343587909 1.1506519735899496 8 0.3673632150948594 0.9317622090672991 9 0.34885136402039907 0.995410855652319 >10 2.176519711327281 13.793257595494257 >50 0.31509950418609717 6.9803073782321405 >100 0.2774754836812743 17.853031897001063 >500 0.03410972872901889 7.526939374996119 >1k 0.019583963251138158 10.762183269447267 >5k 3.855091900680641E-4 0.8741734157714555 >10k+ 2.753585403313707E-4 1.5378376784721104 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28040 0.4861425954709333 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23748 0.41173018392452654 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 14139 0.24513445639670206 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11457 0.19863536791406858 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10693 0.18538954255958237 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8720 0.151182718705654 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7471 0.12952822149655288 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7367 0.12772512485143958 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7306 0.12666753931920965 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 7048 0.12219447264190934 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6655 0.11538084781951004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.507741612783262E-4 0.0 0.0 2 5.547989677271707E-4 0.0 0.0 3 6.06811370951593E-4 0.0 0.0 4 6.588237741760152E-4 0.0 0.0 5 7.628485806248597E-4 0.0 0.0 6 8.495359193322301E-4 0.0 0.0 7 0.0010575855322299192 0.0 0.0 8 0.001248297677386134 0.0 0.0 9 0.0013349850160935045 0.0 0.0 10-11 0.0019331276531743603 0.0 0.0 12-13 0.0028693509112139607 0.0 0.0 14-15 0.0040049550482805125 0.0 0.0 16-17 0.005339940064374018 0.0 0.0 18-19 0.006666256346596785 0.0 1.7337467741474083E-5 20-21 0.008217959709458716 0.0 1.7337467741474083E-5 22-23 0.010211768499728234 0.0 1.7337467741474083E-5 24-25 0.012725701322241977 0.0 1.7337467741474083E-5 26-27 0.014719510112511497 0.0 1.7337467741474083E-5 28-29 0.017649542160820618 0.0 2.6006201612211126E-5 30-31 0.029543045031471836 0.0 3.4674935482948166E-5 32-33 0.033296606797500974 0.0 3.4674935482948166E-5 34-35 0.03681611274902022 0.0 3.4674935482948166E-5 36-37 0.04088174893439589 0.0 3.4674935482948166E-5 38-39 0.04604831432135517 0.0 3.4674935482948166E-5 40-41 0.05177834740991235 0.0 3.4674935482948166E-5 42-43 0.05824522287748218 0.0 3.4674935482948166E-5 44-45 0.0653622533853573 0.0 3.4674935482948166E-5 46-47 0.07307742653031327 0.0 3.4674935482948166E-5 48-49 0.08165947306234293 0.0 3.4674935482948166E-5 50-51 0.09199260383626148 0.0 3.4674935482948166E-5 52-53 0.10362604469079059 0.0 3.4674935482948166E-5 54-55 0.11649044575496437 0.0 3.4674935482948166E-5 56-57 0.1312879744723125 0.0 3.4674935482948166E-5 58-59 0.14841739260088888 0.0 3.4674935482948166E-5 60-61 0.16720253889877607 0.0 3.4674935482948166E-5 62-63 0.18853629295465993 0.0 3.4674935482948166E-5 64-65 0.21234063616370383 0.0 3.4674935482948166E-5 66-67 0.2389189742113836 0.0 3.4674935482948166E-5 68-69 0.2681239386218967 0.0 3.4674935482948166E-5 70-71 0.3014638890887513 0.0 3.4674935482948166E-5 72-73 0.339354924837743 0.0 3.4674935482948166E-5 74-75 0.3812249094334029 0.0 3.4674935482948166E-5 76-77 0.42821811574666835 0.0 4.334366935368521E-5 78-79 0.48165219132589154 0.0 5.201240322442225E-5 80-81 0.5422379723484727 0.0 5.201240322442225E-5 82-83 0.6101835084273096 0.0 5.201240322442225E-5 84-85 0.6861129484010954 0.0 5.201240322442225E-5 86-87 0.7701823294795032 0.0 5.201240322442225E-5 88-89 0.8605365426141953 0.0 5.201240322442225E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 925 0.0 55.903095 9 TTGTGTT 71755 0.0 51.536037 1 CGGGAGA 1195 0.0 50.4348 4 ATTCGGA 285 0.0 48.292713 2 AGAGCGG 1140 0.0 46.611645 8 GTACGTC 45 9.6636685E-4 42.190773 1 CGGAAAG 565 0.0 40.315163 5 CGAGGTG 475 0.0 37.971695 1 CGCAGCC 305 0.0 35.79299 1 CGAGTGG 240 0.0 35.598465 1 TGTGTTT 107240 0.0 34.57717 2 AAGCGGC 330 0.0 34.502327 9 ATGATCG 195 0.0 34.07716 1 GTACGGG 575 0.0 32.193394 1 TCGGAAA 460 0.0 31.981466 4 CGAGGGG 980 0.0 31.965967 1 ACGGCGA 135 1.2076271E-8 31.643078 1 TCGGGAA 405 0.0 31.63813 3 AACCGGA 350 0.0 31.188053 2 GACCGAG 420 0.0 30.512972 1 >>END_MODULE