FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767855
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA280400.4861425954709333No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC237480.41173018392452654No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT141390.24513445639670206No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114570.19863536791406858No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT106930.18538954255958237No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC87200.151182718705654No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC74710.12952822149655288No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT73670.12772512485143958No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG73060.12666753931920965No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT70480.12219447264190934No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA66550.11538084781951004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9250.055.9030959
TTGTGTT717550.051.5360371
CGGGAGA11950.050.43484
ATTCGGA2850.048.2927132
AGAGCGG11400.046.6116458
GTACGTC459.6636685E-442.1907731
CGGAAAG5650.040.3151635
CGAGGTG4750.037.9716951
CGCAGCC3050.035.792991
CGAGTGG2400.035.5984651
TGTGTTT1072400.034.577172
AAGCGGC3300.034.5023279
ATGATCG1950.034.077161
GTACGGG5750.032.1933941
TCGGAAA4600.031.9814664
CGAGGGG9800.031.9659671
ACGGCGA1351.2076271E-831.6430781
TCGGGAA4050.031.638133
AACCGGA3500.031.1880532
GACCGAG4200.030.5129721
Produced by FastQC (version 0.11.2)