FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8369637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC171790.20525382403083908No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG162160.1937479486864245No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA131940.15764124537300722No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA131270.15684073275818294No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC117630.1405437296742977No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG107490.1284285089066587No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC102820.12284881650183872No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA97940.117018217158044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC55250.084.642119
CGGGAGA73750.082.145764
AGAGCGG59000.080.951438
AAGCGGC25150.076.1877759
CGGAAAG30850.076.117225
ATTCGGA10200.075.355562
GAGAGCG73200.075.2300957
TCGGGAG82250.071.6954353
TCGGAGA17650.069.347574
TCGGGAA31300.068.509433
ACCGGAA10900.068.332833
GAACCGG9700.067.998721
AACCGGA9950.064.37412
GTTCGGA9500.063.9273762
AAGCGTC12150.063.2383589
CGGAGAG30950.062.6896065
GGATCGG28700.062.498211
GATCCGG16550.061.6450461
ATCGGGA122050.060.6050532
AGAGCGT177250.060.099468
Produced by FastQC (version 0.11.2)