##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8369637 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08066610296241 33.0 33.0 33.0 27.0 33.0 2 30.75171922031983 33.0 33.0 33.0 27.0 33.0 3 30.590308277407967 33.0 33.0 33.0 27.0 33.0 4 34.04969857115667 37.0 37.0 37.0 27.0 37.0 5 34.086671620286516 37.0 37.0 37.0 27.0 37.0 6 34.479339187589616 37.0 37.0 37.0 27.0 37.0 7 34.045948707213945 37.0 37.0 37.0 27.0 37.0 8 34.30884505504839 37.0 37.0 37.0 27.0 37.0 9 33.80524221062395 37.0 37.0 37.0 22.0 37.0 10-11 33.80221430152825 37.0 37.0 37.0 24.5 37.0 12-13 33.79955026723381 37.0 37.0 37.0 24.5 37.0 14-15 35.336233638328636 40.0 37.0 40.0 22.0 40.0 16-17 35.25015272466416 40.0 37.0 40.0 22.0 40.0 18-19 35.293606341589246 40.0 37.0 40.0 22.0 40.0 20-21 35.31888784423984 40.0 37.0 40.0 22.0 40.0 22-23 35.847521702554126 40.0 37.0 40.0 27.0 40.0 24-25 35.32326802225712 40.0 37.0 40.0 22.0 40.0 26-27 35.18674603211585 37.0 37.0 40.0 24.5 40.0 28-29 34.9192233187652 37.0 37.0 40.0 18.5 40.0 30-31 34.965455909258665 37.0 37.0 40.0 18.5 40.0 32-33 34.752298158211644 37.0 37.0 40.0 15.0 40.0 34-35 34.80664125576772 37.0 37.0 40.0 18.5 40.0 36-37 34.64869969868466 37.0 37.0 40.0 18.5 40.0 38-39 34.764664106699016 37.0 37.0 40.0 18.5 40.0 40-41 34.474746037372945 37.0 37.0 40.0 15.0 40.0 42-43 34.307228079306185 37.0 37.0 40.0 15.0 40.0 44-45 34.19573811863047 37.0 33.0 40.0 15.0 40.0 46-47 34.14871965176029 37.0 33.0 40.0 15.0 40.0 48-49 34.0444875327329 37.0 33.0 40.0 15.0 40.0 50-51 33.02873708859775 37.0 33.0 38.5 15.0 38.5 52-53 33.48879121041928 37.0 33.0 38.5 18.5 40.0 54-55 34.16539038670375 37.0 33.0 40.0 18.5 40.0 56-57 34.356286539069735 37.0 33.0 40.0 18.5 40.0 58-59 33.931995736493704 37.0 33.0 40.0 15.0 40.0 60-61 33.73609954649168 37.0 33.0 40.0 15.0 40.0 62-63 33.431935279869364 37.0 33.0 40.0 15.0 40.0 64-65 33.22409197674881 37.0 33.0 38.5 15.0 40.0 66-67 33.07021791984526 37.0 33.0 37.0 15.0 40.0 68-69 33.05763206934781 37.0 33.0 37.0 18.5 40.0 70-71 32.67557009939618 37.0 33.0 37.0 15.0 40.0 72-73 32.799275344916396 37.0 33.0 37.0 22.0 37.0 74-75 32.75025786661955 37.0 33.0 37.0 22.0 37.0 76-77 32.42757463674948 37.0 33.0 37.0 22.0 37.0 78-79 32.002052359021064 37.0 33.0 37.0 22.0 37.0 80-81 31.602116435874102 37.0 33.0 37.0 15.0 37.0 82-83 31.273210235999482 33.0 33.0 37.0 15.0 37.0 84-85 31.118371860093813 33.0 33.0 37.0 15.0 37.0 86-87 30.96370655023629 33.0 33.0 37.0 15.0 37.0 88-89 30.758694313743835 33.0 33.0 37.0 15.0 37.0 90-91 30.680342887033213 33.0 33.0 37.0 15.0 37.0 92-93 30.504367333971594 33.0 27.0 37.0 15.0 37.0 94-95 30.360433433373515 33.0 27.0 37.0 15.0 37.0 96-97 30.221893135867184 33.0 27.0 37.0 15.0 37.0 98-99 30.085146524275785 33.0 27.0 37.0 15.0 37.0 100-101 28.898262851781983 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1809909512465886 2306 2 0.1438831293167837 2306 3 0.1619083137236501 2306 4 0.26676560162439955 2306 5 0.23090527204050915 2306 6 0.16562113174934012 2306 7 0.5778529469999611 2306 8 0.1702409737163748 2306 9 0.3336469225331342 2306 10-11 0.30122779203916394 2306 12-13 0.2782622041427132 2306 14-15 0.5681399529267495 2306 16-17 0.47096581060519327 2306 18-19 0.4378135730990067 2306 20-21 0.4954622679026315 2306 22-23 0.27666643125858315 2306 24-25 0.5689412796502253 2306 26-27 0.34916369906558486 2306 28-29 0.4537339301852583 2306 30-31 0.5243325059817536 2306 32-33 0.5345565277562443 2306 34-35 0.38242339503275247 2306 36-37 0.4241927023236798 2306 38-39 0.33159417193001417 2306 40-41 0.4514647003366363 2306 42-43 0.3768992995226199 2306 44-45 0.48299208635054214 2306 46-47 0.5118774312350141 2306 48-49 0.5037394739797278 2306 50-51 0.5048164837530962 2306 52-53 0.3823418337888427 2306 54-55 0.5320511042384837 2306 56-57 0.4534522698164096 2306 58-59 0.6094382435232362 2306 60-61 0.5368712153438295 2306 62-63 0.4653762559595336 2306 64-65 0.5196358225818258 2306 66-67 0.5102574687625392 2306 68-69 0.44564407408788753 2306 70-71 0.5736500807820377 2306 72-73 0.2425556884983493 2306 74-75 0.4212509871727619 2306 76-77 0.40847403981174324 2306 78-79 0.3687706220985234 2306 80-81 0.43652138927194883 2306 82-83 0.4927616269160069 2306 84-85 0.4454525239789646 2306 86-87 0.5446019168634528 2306 88-89 0.6426712033226707 2306 90-91 0.5362071438631304 2306 92-93 0.394776471010033 2306 94-95 0.37749036864595453 2306 96-97 0.4113012116029111 2306 98-99 0.3996478067258664 2306 100-101 0.19840681073827682 2307 1 0.18140844931590294 2307 2 0.21808321869665193 2307 3 0.268673056501715 2307 4 0.42563513249769613 2307 5 0.38033660242849265 2307 6 0.1905559589286625 2307 7 0.590316832436919 2307 8 0.15091556631274727 2307 9 0.46406886019460103 2307 10-11 0.3784058383050066 2307 12-13 0.2581417863310307 2307 14-15 0.5012478928383857 2307 16-17 0.5150980761496484 2307 18-19 0.5464262838571301 2307 20-21 0.42061162636686333 2307 22-23 0.3042792733423809 2307 24-25 0.5855714479039094 2307 26-27 0.36108001030353876 2307 28-29 0.5248444848500569 2307 30-31 0.44827293312344096 2307 32-33 0.5253737508256577 2307 34-35 0.36346244090265856 2307 36-37 0.43830384305385195 2307 38-39 0.19824389202990034 2307 40-41 0.44373520380402454 2307 42-43 0.35772802829000483 2307 44-45 0.3613099464475269 2307 46-47 0.28420850622394056 2307 48-49 0.2626014095243008 2307 50-51 0.33583058754350503 2307 52-53 0.40191617196620655 2307 54-55 0.31691356508578394 2307 56-57 0.25532370981233754 2307 58-59 0.35775573120410087 2307 60-61 0.38975050645954923 2307 62-63 0.43128736709243043 2307 64-65 0.3569243049273396 2307 66-67 0.4153883093253228 2307 68-69 0.2869528374311656 2307 70-71 0.3583197672860692 2307 72-73 3.3747029387143357E-4 2307 74-75 0.1529912778925393 2307 76-77 0.29118895821341084 2307 78-79 0.37866194519499174 2307 80-81 0.26332349126692023 2307 82-83 0.32024941558750086 2307 84-85 0.37250262570736936 2307 86-87 0.4003222039374812 2307 88-89 0.508528387621606 2307 90-91 0.4517852120819228 2307 92-93 0.54896019858176 2307 94-95 0.43252070585895197 2307 96-97 0.4634852851296145 2307 98-99 0.3183220984492081 2307 100-101 0.1411420503194485 2201 1 0.22823115775091196 2201 2 0.12780713310207403 2201 3 0.1498064014036622 2201 4 0.2846010774557115 2201 5 0.2751365728116255 2201 6 0.22929126826331014 2201 7 0.5560826221585344 2201 8 0.11207953212130661 2201 9 0.3623934440970942 2201 10-11 0.31267758544114344 2201 12-13 0.3073277580221614 2201 14-15 0.49773511918896673 2201 16-17 0.44740643358488796 2201 18-19 0.44229665642219373 2201 20-21 0.4255578839020302 2201 22-23 0.2732082568167655 2201 24-25 0.49526045016620657 2201 26-27 0.3821700164676045 2201 28-29 0.48540655430803525 2201 30-31 0.4472818683856019 2201 32-33 0.5416306945712606 2201 34-35 0.3667692031074026 2201 36-37 0.26999070474101217 2201 38-39 0.29680823043332083 2201 40-41 0.4052928538844043 2201 42-43 0.35999661843313646 2201 44-45 0.5270339470444512 2201 46-47 0.3342539057639584 2201 48-49 0.4506882882663774 2201 50-51 0.39022959780832167 2201 52-53 0.39635594897778503 2201 54-55 0.5300994322467929 2201 56-57 0.6128106681898657 2201 58-59 0.6268198244431318 2201 60-61 0.5924844390224919 2201 62-63 0.6340140559014458 2201 64-65 0.5650041737328735 2201 66-67 0.4554737993755822 2201 68-69 0.42215403155024234 2201 70-71 0.5037953552622625 2201 72-73 0.3498914407306515 2201 74-75 0.47402003307406204 2201 76-77 0.46604034091078717 2201 78-79 0.45673230542733023 2201 80-81 0.602072692104354 2201 82-83 0.468091785542061 2201 84-85 0.46264103413084356 2201 86-87 0.6312689075595195 2201 88-89 0.6518071926039362 2201 90-91 0.5395185681245742 2201 92-93 0.5118111452785072 2201 94-95 0.41984372387274504 2201 96-97 0.4739478446642167 2201 98-99 0.42882198853212117 2201 100-101 0.47474384191068353 1101 1 0.250258077883192 1101 2 0.14562205110378557 1101 3 0.23971521689454534 1101 4 0.307824483209302 1101 5 0.34350758818835203 1101 6 0.36311393340088216 1101 7 0.5641620504207552 1101 8 0.25535601147375075 1101 9 0.3742426650948403 1101 10-11 0.3033515311963697 1101 12-13 0.3583836706137973 1101 14-15 0.5351916633267777 1101 16-17 0.5558584465589078 1101 18-19 0.48077141522428946 1101 20-21 0.46938749562397675 1101 22-23 0.35410096703301264 1101 24-25 0.47436663866219675 1101 26-27 0.41348354568191326 1101 28-29 0.4316261722670731 1101 30-31 0.447775806537706 1101 32-33 0.5388373946366016 1101 34-35 0.39306551940571666 1101 36-37 0.4750551459592529 1101 38-39 0.3708510895881503 1101 40-41 0.413345597096928 1101 42-43 0.4462754200202568 1101 44-45 0.5826555975147727 1101 46-47 0.3820951699576085 1101 48-49 0.42943078706714033 1101 50-51 0.42319230545135866 1101 52-53 0.4480344597825834 1101 54-55 0.4771942563001943 1101 56-57 0.6140444811128347 1101 58-59 0.5691726516288398 1101 60-61 0.5956200382554044 1101 62-63 0.6014579775871809 1101 64-65 0.5627533639157392 1101 66-67 0.5296562255760691 1101 68-69 0.4182470014162618 1101 70-71 0.547860526083646 1101 72-73 0.4009109790581036 1101 74-75 0.5924166216234212 1101 76-77 0.5550155502878269 1101 78-79 0.3520172583419807 1101 80-81 0.5719974762678355 1101 82-83 0.6578005161067182 1101 84-85 0.6502253911852804 1101 86-87 0.573201656998549 1101 88-89 0.733155348847017 1101 90-91 0.5621397600779758 1101 92-93 0.6724157522617276 1101 94-95 0.6257275655456844 1101 96-97 0.5422959871412019 1101 98-99 0.45461976033047335 1101 100-101 0.5045091087770679 2304 1 0.15055719227919084 2304 2 0.12659439984831877 2304 3 0.10054880066379113 2304 4 0.20407690539416734 2304 5 0.155981096764215 2304 6 0.24779782066715939 2304 7 0.38439672452537366 2304 8 0.14007312062937416 2304 9 0.25629220131883557 2304 10-11 0.21025612763454404 2304 12-13 0.1653010236313932 2304 14-15 0.30953090381725445 2304 16-17 0.3266706689272212 2304 18-19 0.27724519927965474 2304 20-21 0.23935422286482577 2304 22-23 0.26196768738687837 2304 24-25 0.40831733565664763 2304 26-27 0.35590950273841315 2304 28-29 0.3549476451119915 2304 30-31 0.376687400311873 2304 32-33 0.3976196734782107 2304 34-35 0.350034466134872 2304 36-37 0.3976150979694708 2304 38-39 0.2855732387665668 2304 40-41 0.39038805850631775 2304 42-43 0.33549069081974636 2304 44-45 0.4104255752572783 2304 46-47 0.39779394094334464 2304 48-49 0.40300823280863085 2304 50-51 0.34586154926011403 2304 52-53 0.3705325658609766 2304 54-55 0.5218689517857698 2304 56-57 0.5040548859425016 2304 58-59 0.5745445020059066 2304 60-61 0.44101162611301703 2304 62-63 0.4108362939065202 2304 64-65 0.42276939181430606 2304 66-67 0.44837912762745447 2304 68-69 0.3606781432788111 2304 70-71 0.4795112626782796 2304 72-73 0.40477731827981245 2304 74-75 0.5258231487659799 2304 76-77 0.5432062642806272 2304 78-79 0.5811534226968078 2304 80-81 0.4398195916373986 2304 82-83 0.5009344241904756 2304 84-85 0.4785403213919217 2304 86-87 0.29193177864779685 2304 88-89 0.4314837673755214 2304 90-91 0.5010106227984004 2304 92-93 0.5392023151227079 2304 94-95 0.46475493114768796 2304 96-97 0.5471278742071277 2304 98-99 0.4292499173537898 2304 100-101 0.4467088908978205 2202 1 0.15048267675940963 2202 2 0.040791108173735324 2202 3 0.12381778070800564 2202 4 0.18134573770076656 2202 5 0.16569035402053345 2202 6 0.2086098070166642 2202 7 0.34799998895184814 2202 8 0.21796740186143637 2202 9 0.2436183114132504 2202 10-11 0.15656597107003023 2202 12-13 0.20218915205160215 2202 14-15 0.2714143112445484 2202 16-17 0.23578788169611187 2202 18-19 0.28288807923191683 2202 20-21 0.2627007274330495 2202 22-23 0.17612910503278556 2202 24-25 0.29244306166201284 2202 26-27 0.3456780619891049 2202 28-29 0.3635762925999799 2202 30-31 0.35861179091575224 2202 32-33 0.36613659690844713 2202 34-35 0.3147562986935455 2202 36-37 0.33543982790999394 2202 38-39 0.2669067597419925 2202 40-41 0.27661999765451384 2202 42-43 0.2141848408268885 2202 44-45 0.3209885202485907 2202 46-47 0.32585486532094876 2202 48-49 0.3498416609450672 2202 50-51 0.3199481934202666 2202 52-53 0.2713708223444016 2202 54-55 0.39336128818433025 2202 56-57 0.4058316733017904 2202 58-59 0.3916740032247432 2202 60-61 0.30457266747351497 2202 62-63 0.37961635700255414 2202 64-65 0.4166143523950865 2202 66-67 0.42869467103314207 2202 68-69 0.5182376619214892 2202 70-71 0.41578092126642474 2202 72-73 0.3652585476982253 2202 74-75 0.5361987073449512 2202 76-77 0.41548275616467834 2202 78-79 0.4040320711189409 2202 80-81 0.425739823096837 2202 82-83 0.3994068847777257 2202 84-85 0.4168926779793658 2202 86-87 0.3735562101525822 2202 88-89 0.5728582833839262 2202 90-91 0.5075795215581778 2202 92-93 0.47969322940298653 2202 94-95 0.46182503228759586 2202 96-97 0.3716337831369465 2202 98-99 0.4247983964898765 2202 100-101 0.5512782731102668 1102 1 0.06299777687350527 1102 2 0.02697009491308222 1102 3 0.12277929861302184 1102 4 0.14624716134374438 1102 5 0.1970749025663565 1102 6 0.16102296485248502 1102 7 0.2360155309546812 1102 8 0.12342336519689923 1102 9 0.17182972981271405 1102 10-11 0.21691063193043192 1102 12-13 0.19524993970103566 1102 14-15 0.2620414240401274 1102 16-17 0.21186177808807827 1102 18-19 0.27283490114489695 1102 20-21 0.26919693429319125 1102 22-23 0.18343492657146498 1102 24-25 0.27540213461462315 1102 26-27 0.3005779152354435 1102 28-29 0.26869949631606005 1102 30-31 0.32014290242577914 1102 32-33 0.3001826538787 1102 34-35 0.2949337594357857 1102 36-37 0.28181556279292863 1102 38-39 0.2722597378162277 1102 40-41 0.21507547491321333 1102 42-43 0.22240478684727805 1102 44-45 0.3751323732845009 1102 46-47 0.3122346821183868 1102 48-49 0.2906321831470322 1102 50-51 0.21730982892990625 1102 52-53 0.2806294958078013 1102 54-55 0.44247119173564187 1102 56-57 0.4564644690347066 1102 58-59 0.3591963361143442 1102 60-61 0.32057551005893714 1102 62-63 0.4717267611146312 1102 64-65 0.4133751830176493 1102 66-67 0.4125045263727216 1102 68-69 0.37249532768128546 1102 70-71 0.41550127526674885 1102 72-73 0.4153514846883368 1102 74-75 0.47528209791065024 1102 76-77 0.3961919368631541 1102 78-79 0.34138709475281104 1102 80-81 0.48191429492469595 1102 82-83 0.44780243637078954 1102 84-85 0.5546518947363879 1102 86-87 0.34590916668694405 1102 88-89 0.4728073000619908 1102 90-91 0.5653079009435338 1102 92-93 0.4227844816642694 1102 94-95 0.3920693537538291 1102 96-97 0.498836799110677 1102 98-99 0.5403167968396332 1102 100-101 0.40878348392184805 2305 1 0.20415138721888226 2305 2 0.0962131858580264 2305 3 0.1049288018319885 2305 4 0.0619744181740387 2305 5 0.03944683245880043 2305 6 0.28075455866019183 2305 7 0.07087098777723355 2305 8 0.24731676440167405 2305 9 0.03791619289690118 2305 10-11 0.11859338224856941 2305 12-13 0.09292623690220836 2305 14-15 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sequence quality scores pass #Quality Count 2 6341.0 3 921.0 4 1014.0 5 798.0 6 1521.0 7 10412.0 8 49659.0 9 135524.0 10 170179.0 11 122828.0 12 76497.0 13 51698.0 14 36791.0 15 29842.0 16 27068.0 17 26434.0 18 27170.0 19 28865.0 20 31384.0 21 34522.0 22 38065.0 23 40478.0 24 44897.0 25 51677.0 26 60193.0 27 71497.0 28 87068.0 29 107459.0 30 132553.0 31 172270.0 32 234751.0 33 331672.0 34 502844.0 35 852618.0 36 1649417.0 37 2522497.0 38 599945.0 39 268.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.69789085490066 23.727316343014447 25.693332111439144 13.881460690645742 2 29.497671828071613 25.01990399846685 33.58324075543565 11.89918341802589 3 36.73205943517047 20.119657247732434 28.157720930188006 14.99056238690909 4 26.771486097415973 18.159960390372753 36.957721991425494 18.110831520785776 5 33.74167123138714 17.144335622998717 33.10177606148014 16.012217084134008 6 27.254523778015567 17.622659518601395 40.24663340137688 14.876183302006158 7 33.74277598526054 19.14405213407465 31.196237453909266 15.916934426755544 8 26.67867359769015 30.23695709902713 27.48693249531452 15.5974368079682 9 32.87691139753262 26.453411469474496 23.902575136168856 16.767101996824024 10-11 26.51261078259929 29.250496129023645 27.39294693368894 16.843946154688126 12-13 28.274001812016596 24.18563262994724 27.161188963041027 20.379176594995137 14-15 25.39169836041708 24.837696232282706 28.738254316846835 21.03235109045337 16-17 25.73924495039815 26.303914423521558 29.83165150451722 18.125189121563075 18-19 25.22987596215106 26.72458746826748 29.988281443595906 18.057255125985556 20-21 24.320724042266047 28.613338318174996 28.95380156726822 18.11213607229074 22-23 26.90721330565968 26.718772466467577 28.28311924189017 18.09089498598257 24-25 23.875313772473348 29.375789795520763 28.497436942101405 18.25145948990448 26-27 22.35421977310526 30.719703909934477 28.461988391367804 18.46408792559246 28-29 22.991342225770655 29.63225785272726 28.15020826149871 19.22619166000337 30-31 24.870547085109386 27.585287242955737 28.358542191064945 19.18562348086994 32-33 22.520124639448337 27.108522656479376 29.899293948317645 20.472058755754638 34-35 20.292300691693086 27.29691347714705 33.24318831923896 19.167597511920903 36-37 21.28610988666459 28.015829756471373 29.96226227860189 20.735798078262146 38-39 20.59080897353143 27.622109593979093 27.861146746574285 23.92593468591519 40-41 22.069606609855008 27.095230120065512 28.823652681222484 22.011510588857 42-43 23.645915315009013 27.283051938492793 30.298106734857434 18.772926011640763 44-45 21.654350306849487 29.21012070110548 30.5788901914684 18.556638800576636 46-47 23.033452094796438 29.650228993710204 28.81574489075978 18.50057402073358 48-49 21.0565835325306 28.88270416250948 30.12492060283578 19.93579170212414 50-51 20.21453823752016 28.030549268420963 30.41774973964577 21.3371627544131 52-53 23.99550098108037 27.625813088077926 28.132045641744785 20.246640289096916 54-55 25.189573966767565 27.534510208838693 27.984267196335967 19.291648628057782 56-57 25.449794381253238 27.170646951058863 27.40971704322964 19.96984162445825 58-59 23.923185578258323 26.87657646223664 28.027039806418998 21.17319815308604 60-61 21.005724547267892 27.174707975812574 27.9651316764535 23.854435800466035 62-63 23.114620747952657 27.328252105224877 28.315785348850003 21.24134179797247 64-65 21.18233322211932 28.583745831871255 29.994072580567842 20.23984836544159 66-67 20.521658202982206 28.515878846861447 28.636553768267238 22.325909181889113 68-69 20.272014910837626 27.29139360595954 32.278103902408326 20.158487580794507 70-71 19.856625850618933 31.20181064328131 29.736103439240797 19.205460066858965 72-73 19.816329159813165 34.025244853507346 27.154887632538475 19.003538354141014 74-75 20.621958170211606 33.44520164458341 26.828321559487684 19.1045186257173 76-77 20.8309486956194 32.46690414472456 27.380778251073394 19.321368908582652 78-79 20.773478120388916 32.229143875904484 27.850609010712336 19.146768992994268 80-81 20.024565592132777 32.75374725968506 27.62935083618787 19.592336311994288 82-83 19.34943103448896 32.15485654266879 28.12135581117852 20.37435661166373 84-85 19.463129246562964 31.42480120143772 28.447766320243336 20.66430323175598 86-87 19.387160812397024 31.257589502632143 29.124532401519144 20.230717283451686 88-89 19.680773429525715 31.528111798529697 28.699630431508094 20.091484340436494 90-91 20.23225226074963 30.721128600155435 28.287062873849074 20.75955626524587 92-93 19.828138818816715 31.496307652616355 27.963280057178014 20.712273471388915 94-95 19.54561562224104 32.09080904571934 27.847849296972548 20.515726035067065 96-97 20.06260184827871 32.31432780570421 27.34804112497117 20.275029221045912 98-99 20.466770196291808 31.23371737970374 27.208064883891716 21.091447540112735 100-101 20.526303423237323 31.27510294718265 26.84290471510144 21.355688914478584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4358.0 1 2841.5 2 1072.5 3 845.5 4 822.0 5 781.0 6 935.5 7 1292.5 8 1709.0 9 2178.5 10 2695.5 11 3051.0 12 3170.0 13 3025.5 14 2756.5 15 2530.0 16 2447.5 17 2485.0 18 2628.5 19 2952.0 20 3460.0 21 4278.5 22 5493.5 23 7291.5 24 9613.0 25 12493.5 26 16583.5 27 21943.5 28 27611.0 29 33854.0 30 41311.0 31 49661.5 32 61396.5 33 85725.0 34 126173.0 35 202284.0 36 304377.0 37 409577.5 38 530529.5 39 594133.5 40 589333.0 41 537832.5 42 481746.0 43 485696.0 44 476413.5 45 450171.0 46 453587.0 47 413313.0 48 327352.0 49 276111.0 50 236938.5 51 201849.0 52 180009.0 53 155137.5 54 122664.0 55 90271.0 56 68796.0 57 54027.0 58 42819.0 59 33267.5 60 25332.5 61 19498.5 62 14538.0 63 10819.0 64 8280.5 65 6152.0 66 4471.0 67 3289.0 68 2436.5 69 1707.0 70 1207.0 71 930.0 72 708.0 73 523.0 74 404.0 75 320.5 76 263.0 77 213.0 78 164.5 79 143.0 80 115.0 81 100.0 82 83.5 83 64.5 84 62.5 85 45.5 86 39.5 87 36.5 88 27.5 89 27.0 90 23.5 91 15.0 92 12.0 93 14.0 94 13.0 95 11.0 96 10.0 97 6.0 98 2.5 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26216190738021256 2 0.24866072447347476 3 0.21445374512658075 4 0.2166402198805038 5 0.23134814568421544 6 0.234084226113988 7 0.21067819309248417 8 0.20597070099933845 9 0.1872363162225554 10-11 0.1756766751055034 12-13 0.20388578381595282 14-15 0.2004985401397934 16-17 0.20161567341570488 18-19 0.19378379253484948 20-21 0.19490092581076096 22-23 0.16050277927226714 24-25 0.46785780554162626 26-27 0.32630447413669195 28-29 0.273835054017277 30-31 0.2767861975375993 32-33 0.3189206413611486 34-35 0.31558716345762666 36-37 0.3074326879409465 38-39 0.33260104351001124 40-41 0.33781632345584406 42-43 0.3735466663607992 44-45 0.3404627942645541 46-47 0.36623452128210576 48-49 0.3424162840037148 50-51 0.36462154810298225 52-53 0.3536174866365172 54-55 0.3294826286970391 56-57 0.3663241309031682 58-59 0.35321125635436756 60-61 0.3190879126537985 62-63 0.2963569387776316 64-65 0.30305973843310047 66-67 0.31240303492254207 68-69 0.31915362637591094 70-71 0.2958013591270446 72-73 0.30302389458467555 74-75 0.3005267731444028 76-77 0.3038064852752873 78-79 0.3060228299028978 80-81 0.323478784085857 82-83 0.33987137076554214 84-85 0.33411843309333483 86-87 0.3386765758180432 88-89 0.33312675328691077 90-91 0.30800021554100854 92-93 0.29238424557719767 94-95 0.301398973456077 96-97 0.28909855947157564 98-99 0.3083706019747332 100-101 0.33141222253724983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8369637.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.02519178438068 #Duplication Level Percentage of deduplicated Percentage of total 1 91.44290437951072 39.34348498249234 2 3.869823414031315 3.3299978912076833 3 1.4962047618341763 1.931234904798572 4 0.5922451284190224 1.019258409343744 5 0.3703657539987733 0.7967528798081989 6 0.34175562082248934 0.8822460677566613 7 0.20834928214355186 0.6274987475655054 8 0.17931854583995158 0.6172171860208141 9 0.13198818198722817 0.5110935158945008 >10 1.0309656301202321 8.97364103104797 >50 0.1470910708454626 4.415830072773403 >100 0.14502958606482083 13.310807734424534 >500 0.023850715252634325 7.084456343663935 >1k 0.01948484213069354 14.912767464530397 >5k 4.250286104310204E-4 1.1175360708566195 >10k+ 1.9805838834289648E-4 1.1261766978152612 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 17179 0.20525382403083908 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 16216 0.1937479486864245 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13194 0.15764124537300722 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13127 0.15684073275818294 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11763 0.1405437296742977 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10749 0.1284285089066587 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10282 0.12284881650183872 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9794 0.117018217158044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002198422703398009 0.0 0.0 2 0.0023298501476228897 0.0 0.0 3 0.002532965288697706 0.0 0.0 4 0.0027002365813475543 0.0 0.0 5 0.0029630914697973162 0.0 0.0 6 0.003417113549846905 0.0 1.1947949474989179E-5 7 0.0037994479330465585 0.0 1.1947949474989179E-5 8 0.0043371056594210715 0.0 1.1947949474989179E-5 9 0.004922555183695542 0.0 1.1947949474989179E-5 10-11 0.007939412426130308 0.0 1.1947949474989179E-5 12-13 0.010603805159052897 0.0 1.1947949474989179E-5 14-15 0.013578844578325202 0.0 1.1947949474989179E-5 16-17 0.015938564599635562 0.0 1.1947949474989179E-5 18-19 0.018794124524157976 0.0 2.3895898949978357E-5 20-21 0.022605520406679527 0.0 2.3895898949978357E-5 22-23 0.026709641051338308 0.0 2.3895898949978357E-5 24-25 0.03166206610872132 0.0 2.3895898949978357E-5 26-27 0.03707448722089142 0.0 2.3895898949978357E-5 28-29 0.04283937284257369 0.0 2.3895898949978357E-5 30-31 0.06755968030632631 0.0 2.3895898949978357E-5 32-33 0.07453728279972 0.0 2.9869873687472943E-5 34-35 0.08098917551621415 0.0 3.584384842496753E-5 36-37 0.08780548069169547 0.0 3.584384842496753E-5 38-39 0.09524307923987624 0.0 3.584384842496753E-5 40-41 0.10349911232709375 0.0 3.584384842496753E-5 42-43 0.11189254683327365 0.0 4.1817823162462126E-5 44-45 0.12074000341950314 0.0 4.7791797899956715E-5 46-47 0.13047758224161932 0.0 4.7791797899956715E-5 48-49 0.1409917777796098 0.0 4.7791797899956715E-5 50-51 0.15197791732186233 0.0 6.571372211244048E-5 52-53 0.1638302832010516 0.0 8.363564632492425E-5 54-55 0.17683562620457732 0.0 8.363564632492425E-5 56-57 0.18997239665232793 0.0 8.363564632492425E-5 58-59 0.20485954169816445 0.0 8.363564632492425E-5 60-61 0.22100719541361233 0.0 8.363564632492425E-5 62-63 0.23866626473764632 0.0 8.363564632492425E-5 64-65 0.25707208090386713 0.0 8.363564632492425E-5 66-67 0.27765242387453604 0.0 8.363564632492425E-5 68-69 0.3002280744075281 0.0 1.0753154527490261E-4 70-71 0.32387306641853164 0.0 1.0753154527490261E-4 72-73 0.3502362169350953 0.0 1.0753154527490261E-4 74-75 0.3799208974057059 0.0 1.0753154527490261E-4 76-77 0.4121863349629141 0.0 1.0753154527490261E-4 78-79 0.44917121256274317 0.0 1.0753154527490261E-4 80-81 0.4906365712156931 0.0 1.0753154527490261E-4 82-83 0.5375083770060757 0.0 1.0753154527490261E-4 84-85 0.5881736567547673 0.0 1.0753154527490261E-4 86-87 0.6464139364706021 0.0 1.4337539369987012E-4 88-89 0.7116198707303554 0.0 1.4337539369987012E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5525 0.0 84.64211 9 CGGGAGA 7375 0.0 82.14576 4 AGAGCGG 5900 0.0 80.95143 8 AAGCGGC 2515 0.0 76.187775 9 CGGAAAG 3085 0.0 76.11722 5 ATTCGGA 1020 0.0 75.35556 2 GAGAGCG 7320 0.0 75.230095 7 TCGGGAG 8225 0.0 71.695435 3 TCGGAGA 1765 0.0 69.34757 4 TCGGGAA 3130 0.0 68.50943 3 ACCGGAA 1090 0.0 68.33283 3 GAACCGG 970 0.0 67.99872 1 AACCGGA 995 0.0 64.3741 2 GTTCGGA 950 0.0 63.927376 2 AAGCGTC 1215 0.0 63.238358 9 CGGAGAG 3095 0.0 62.689606 5 GGATCGG 2870 0.0 62.49821 1 GATCCGG 1655 0.0 61.645046 1 ATCGGGA 12205 0.0 60.605053 2 AGAGCGT 17725 0.0 60.09946 8 >>END_MODULE