##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34298-1.342000000357ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5319493 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.669813269798457 33.0 33.0 33.0 27.0 33.0 2 31.560705691313064 33.0 33.0 33.0 27.0 33.0 3 31.5031283996426 33.0 33.0 33.0 27.0 33.0 4 35.15196598623215 37.0 37.0 37.0 33.0 37.0 5 35.14865326451224 37.0 37.0 37.0 33.0 37.0 6 35.270607368033005 37.0 37.0 37.0 33.0 37.0 7 35.12130592144778 37.0 37.0 37.0 33.0 37.0 8 35.149511241014885 37.0 37.0 37.0 33.0 37.0 9 34.9398015938737 37.0 37.0 37.0 33.0 37.0 10-11 34.87727345444387 37.0 37.0 37.0 33.0 37.0 12-13 34.87340071694803 37.0 37.0 37.0 33.0 37.0 14-15 36.46659935448736 40.0 37.0 40.0 33.0 40.0 16-17 36.395657349300016 40.0 37.0 40.0 33.0 40.0 18-19 36.40489995945102 40.0 37.0 40.0 33.0 40.0 20-21 36.39380670300722 40.0 37.0 40.0 33.0 40.0 22-23 36.54021012904801 40.0 37.0 40.0 33.0 40.0 24-25 36.22829074124169 40.0 37.0 40.0 33.0 40.0 26-27 36.09882407966323 40.0 37.0 40.0 27.0 40.0 28-29 35.96775500973495 40.0 37.0 40.0 27.0 40.0 30-31 35.985704840668085 40.0 37.0 40.0 27.0 40.0 32-33 35.82020908759537 37.0 37.0 40.0 27.0 40.0 34-35 35.785871228705446 37.0 37.0 40.0 27.0 40.0 36-37 35.66170093653662 37.0 37.0 40.0 27.0 40.0 38-39 35.652285659554394 37.0 37.0 40.0 27.0 40.0 40-41 35.48249626421165 37.0 37.0 40.0 27.0 40.0 42-43 35.33069852709647 37.0 37.0 40.0 27.0 40.0 44-45 35.22087621884266 37.0 37.0 40.0 27.0 40.0 46-47 35.140542247165286 37.0 37.0 40.0 27.0 40.0 48-49 35.00583457859612 37.0 37.0 40.0 27.0 40.0 50-51 33.88033502440928 37.0 33.0 38.5 22.0 38.5 52-53 34.36214861077926 37.0 33.0 38.5 27.0 40.0 54-55 35.110532620307985 37.0 37.0 40.0 27.0 40.0 56-57 35.19038496713878 37.0 37.0 40.0 27.0 40.0 58-59 34.87966832553403 37.0 33.0 40.0 27.0 40.0 60-61 34.62710468836034 37.0 33.0 40.0 27.0 40.0 62-63 34.37073899711871 37.0 33.0 40.0 27.0 40.0 64-65 34.12149945492926 37.0 33.0 37.0 27.0 40.0 66-67 33.89786658239798 37.0 33.0 37.0 27.0 40.0 68-69 33.7136874698397 37.0 33.0 37.0 22.0 40.0 70-71 33.356202649387825 37.0 33.0 37.0 22.0 40.0 72-73 33.213436882048725 37.0 33.0 37.0 22.0 37.0 74-75 33.02686506026044 37.0 33.0 37.0 24.5 37.0 76-77 32.743944394700776 37.0 33.0 37.0 22.0 37.0 78-79 32.43511731287173 37.0 33.0 37.0 22.0 37.0 80-81 32.16228698862843 37.0 33.0 37.0 22.0 37.0 82-83 31.933292138931286 33.0 33.0 37.0 22.0 37.0 84-85 31.78726412460736 33.0 33.0 37.0 22.0 37.0 86-87 31.64054083725648 33.0 33.0 37.0 18.5 37.0 88-89 31.429937683910854 33.0 33.0 37.0 15.0 37.0 90-91 31.34762382430055 33.0 33.0 37.0 15.0 37.0 92-93 31.15840654363113 33.0 33.0 37.0 15.0 37.0 94-95 31.01282359051887 33.0 33.0 37.0 15.0 37.0 96-97 30.863747353366193 33.0 30.0 37.0 15.0 37.0 98-99 30.71699417594872 33.0 27.0 37.0 15.0 37.0 100-101 29.468765914345596 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.29524335730452833 2306 2 0.34868357199522393 2306 3 0.25394086824889683 2306 4 0.3603172243152102 2306 5 0.39994798482046434 2306 6 0.3717625509409004 2306 7 0.5037707900844453 2306 8 0.39926535363070315 2306 9 0.47430041471288575 2306 10-11 0.5202596011079592 2306 12-13 0.49285290326468356 2306 14-15 0.679044877915203 2306 16-17 0.6080911366901276 2306 18-19 0.6369119123764833 2306 20-21 0.6193701639081794 2306 22-23 0.5483980674638502 2306 24-25 0.6833230096105254 2306 26-27 0.6077922632745256 2306 28-29 0.485541684317532 2306 30-31 0.41520096074101787 2306 32-33 0.4954512527161796 2306 34-35 0.4413527390247083 2306 36-37 0.4321264140066958 2306 38-39 0.4321732250482455 2306 40-41 0.4778505117265155 2306 42-43 0.4534398577539491 2306 44-45 0.4865738151540526 2306 46-47 0.3931138364959992 2306 48-49 0.45005025824874423 2306 50-51 0.4778193478680137 2306 52-53 0.46451901491576564 2306 54-55 0.5347466539831416 2306 56-57 0.5073846440734258 2306 58-59 0.5780434591725694 2306 60-61 0.5926640991471075 2306 62-63 0.4811209212960108 2306 64-65 0.5712562656547675 2306 66-67 0.517622570437716 2306 68-69 0.548495308824748 2306 70-71 0.6169628914694272 2306 72-73 0.5290374336396937 2306 74-75 0.5922947591708336 2306 76-77 0.5194378016497652 2306 78-79 0.5455725792408259 2306 80-81 0.5244120551801359 2306 82-83 0.5809898055459364 2306 84-85 0.5531744008599198 2306 86-87 0.4884648074687341 2306 88-89 0.5558101255989527 2306 90-91 0.4619899646130037 2306 92-93 0.39815990979250415 2306 94-95 0.407092494357574 2306 96-97 0.3970818844905182 2306 98-99 0.4319927054370645 2306 100-101 0.21224112781605697 2307 1 0.22241999733880036 2307 2 0.13663766530289934 2307 3 0.20387059552464493 2307 4 0.23801819277348102 2307 5 0.3278199888606892 2307 6 0.29788901925341094 2307 7 0.34073558901028633 2307 8 0.22734306908283486 2307 9 0.28187750714019444 2307 10-11 0.2963949079435295 2307 12-13 0.21513236284851445 2307 14-15 0.3680344586979061 2307 16-17 0.4250530568279913 2307 18-19 0.44184871706855944 2307 20-21 0.31832431511886483 2307 22-23 0.3835230865119357 2307 24-25 0.4196139430699404 2307 26-27 0.3868733925764971 2307 28-29 0.31824388182931074 2307 30-31 0.24225542383096865 2307 32-33 0.28636156675268154 2307 34-35 0.35347741915278164 2307 36-37 0.3393481550526971 2307 38-39 0.19255371277742483 2307 40-41 0.34130303677035556 2307 42-43 0.21187534257926188 2307 44-45 0.17739597092710113 2307 46-47 0.1133907596427548 2307 48-49 0.07425417757026054 2307 50-51 0.20321934184019597 2307 52-53 0.18942501224821484 2307 54-55 0.0864386861641222 2307 56-57 0.11503832999618169 2307 58-59 0.20890838928014688 2307 60-61 0.22757788567543003 2307 62-63 0.2780836310684123 2307 64-65 0.11579690604943949 2307 66-67 0.18418832469468072 2307 68-69 0.16436689461202292 2307 70-71 0.15041373220589804 2307 72-73 -0.005997347492453287 2307 74-75 0.13511406486988164 2307 76-77 0.22856731243052053 2307 78-79 0.15860108478408108 2307 80-81 0.13474527304173023 2307 82-83 0.18650089518724045 2307 84-85 0.22140105321111392 2307 86-87 0.14579220700106887 2307 88-89 0.26245205003124283 2307 90-91 0.29667192267232423 2307 92-93 0.22228895106795932 2307 94-95 0.10345621143130401 2307 96-97 0.2265060693604326 2307 98-99 0.21655559283502512 2307 100-101 0.06299484085347018 2201 1 0.32231689927343155 2201 2 0.25956719184585 2201 3 0.2796864835182937 2201 4 0.32657153006434925 2201 5 0.38439199579420347 2201 6 0.4008394332352694 2201 7 0.522610158536061 2201 8 0.35024025813667237 2201 9 0.47742364294168027 2201 10-11 0.4965190055378699 2201 12-13 0.39935831916899645 2201 14-15 0.6116899003707275 2201 16-17 0.5589437522461935 2201 18-19 0.5773400438018399 2201 20-21 0.523149574387908 2201 22-23 0.4905591091356811 2201 24-25 0.5808048445106735 2201 26-27 0.6144020242736801 2201 28-29 0.5936802556619867 2201 30-31 0.4730508858892932 2201 32-33 0.6049724067452402 2201 34-35 0.4480846617484673 2201 36-37 0.48952000748046487 2201 38-39 0.5483654117767003 2201 40-41 0.6422306515328557 2201 42-43 0.565565389829203 2201 44-45 0.6355859285793741 2201 46-47 0.5259928924191968 2201 48-49 0.5570091203579537 2201 50-51 0.5406720513622005 2201 52-53 0.49235809690691923 2201 54-55 0.5753249061631109 2201 56-57 0.5919220842120225 2201 58-59 0.678086100001174 2201 60-61 0.6539876055310998 2201 62-63 0.7187993172354652 2201 64-65 0.6230095548483874 2201 66-67 0.5969687352389386 2201 68-69 0.5874082582779963 2201 70-71 0.6092659140217407 2201 72-73 0.5773035284914414 2201 74-75 0.6035919070189877 2201 76-77 0.515861600494155 2201 78-79 0.513264505335556 2201 80-81 0.6200095206691287 2201 82-83 0.5492912425657757 2201 84-85 0.6053485004267323 2201 86-87 0.6829471636363458 2201 88-89 0.7311390195160072 2201 90-91 0.7230820889043805 2201 92-93 0.5681082171388852 2201 94-95 0.5762567376793015 2201 96-97 0.571121706312006 2201 98-99 0.5981192413707568 2201 100-101 0.5706620248042356 1101 1 0.2793254744674556 1101 2 0.19805000102756765 1101 3 0.17305046414084302 1101 4 0.31735357735296077 1101 5 0.3555356839081085 1101 6 0.2862230247478692 1101 7 0.4079229678419978 1101 8 0.2945303020567991 1101 9 0.29674629472439307 1101 10-11 0.3500728934146409 1101 12-13 0.35364583414868633 1101 14-15 0.5689657604272753 1101 16-17 0.5117707396734446 1101 18-19 0.5015643868339836 1101 20-21 0.38871156987425337 1101 22-23 0.42031730821134516 1101 24-25 0.45257900989587796 1101 26-27 0.44069013738646134 1101 28-29 0.4075545994324159 1101 30-31 0.461552574232428 1101 32-33 0.573290548857635 1101 34-35 0.43707552076555345 1101 36-37 0.5798108450050634 1101 38-39 0.430057289330982 1101 40-41 0.5271425101071969 1101 42-43 0.47158921343243776 1101 44-45 0.5037749283213273 1101 46-47 0.37625950930393515 1101 48-49 0.46101785422599306 1101 50-51 0.5094879189516632 1101 52-53 0.5146963988230269 1101 54-55 0.48874861103036693 1101 56-57 0.6010865987011655 1101 58-59 0.6814003713581229 1101 60-61 0.6213335938792213 1101 62-63 0.5793783859994406 1101 64-65 0.5325403789759307 1101 66-67 0.5001806302122063 1101 68-69 0.44176402418013794 1101 70-71 0.5054378388454097 1101 72-73 0.49033671245557287 1101 74-75 0.6206725675622948 1101 76-77 0.573570286713128 1101 78-79 0.49301957546266806 1101 80-81 0.6322840693798213 1101 82-83 0.5967932986482545 1101 84-85 0.6212607623659032 1101 86-87 0.47724446156076183 1101 88-89 0.7115883412138686 1101 90-91 0.7873488168843714 1101 92-93 0.647584306932913 1101 94-95 0.6467389637281364 1101 96-97 0.6023006355272216 1101 98-99 0.4775651666945926 1101 100-101 0.554237201249979 2304 1 0.3069778522407631 2304 2 0.238133569444269 2304 3 0.23560093988206177 2304 4 0.31289461111568073 2304 5 0.3378854870688244 2304 6 0.3308777153772695 2304 7 0.3959953020055096 2304 8 0.4394571874653721 2304 9 0.3946709427878261 2304 10-11 0.40066414418252094 2304 12-13 0.3811115221934003 2304 14-15 0.4514956794217895 2304 16-17 0.49446576114743124 2304 18-19 0.48192314381707746 2304 20-21 0.42209869365758124 2304 22-23 0.4996785654953726 2304 24-25 0.5964799013249049 2304 26-27 0.678454786622531 2304 28-29 0.5797951515685114 2304 30-31 0.5751004164180813 2304 32-33 0.5603052632774279 2304 34-35 0.5531699254482945 2304 36-37 0.5339550638198247 2304 38-39 0.4664403546226339 2304 40-41 0.49375116659409457 2304 42-43 0.4386286154922914 2304 44-45 0.5171710156990414 2304 46-47 0.46504342316104186 2304 48-49 0.5626145999703951 2304 50-51 0.5343151432267419 2304 52-53 0.589555048980337 2304 54-55 0.6388007357975596 2304 56-57 0.5842178291876934 2304 58-59 0.6440813255856099 2304 60-61 0.5474220953821103 2304 62-63 0.4700744370874901 2304 64-65 0.6380620952599543 2304 66-67 0.5446644662041962 2304 68-69 0.5822935736151607 2304 70-71 0.6447540160900544 2304 72-73 0.5213720031660785 2304 74-75 0.70434792109792 2304 76-77 0.7280104418392739 2304 78-79 0.7320342641199034 2304 80-81 0.47669044001690253 2304 82-83 0.6139963818049132 2304 84-85 0.5803556347723777 2304 86-87 0.5210825678526483 2304 88-89 0.5619127940148587 2304 90-91 0.7024048793257158 2304 92-93 0.6741647622994975 2304 94-95 0.6620055684758235 2304 96-97 0.6227921763015836 2304 98-99 0.6051782787719446 2304 100-101 0.5126253577566473 2202 1 0.2241681036408245 2202 2 0.1720158132926457 2202 3 0.1352227203718428 2202 4 0.21618664516496722 2202 5 0.2531258914556602 2202 6 0.266216787104014 2202 7 0.34039846304413857 2202 8 0.23991305153499098 2202 9 0.23293208573479518 2202 10-11 0.25764929644683576 2202 12-13 0.30637188551912686 2202 14-15 0.35118653022514934 2202 16-17 0.28920149177638166 2202 18-19 0.25887763521345875 2202 20-21 0.27187790682761914 2202 22-23 0.29559801839497624 2202 24-25 0.4011359549806244 2202 26-27 0.4280078143633901 2202 28-29 0.38409950236313506 2202 30-31 0.36660214793939616 2202 32-33 0.38596742528938677 2202 34-35 0.3424816516821494 2202 36-37 0.31278120193186254 2202 38-39 0.25632885767046787 2202 40-41 0.28394254693652954 2202 42-43 0.2564524877804857 2202 44-45 0.24695842203731644 2202 46-47 0.2043085467810215 2202 48-49 0.23103501747265653 2202 50-51 0.24349617572472226 2202 52-53 0.2659896076369108 2202 54-55 0.2951687805360663 2202 56-57 0.30720577919666425 2202 58-59 0.275359777394371 2202 60-61 0.2645227935480179 2202 62-63 0.41990517512363823 2202 64-65 0.4552448838905008 2202 66-67 0.4625767802502381 2202 68-69 0.5030028384053438 2202 70-71 0.5032571274298121 2202 72-73 0.4780279996601706 2202 74-75 0.578271178422149 2202 76-77 0.5503870004863174 2202 78-79 0.4978668472067298 2202 80-81 0.4369555588734215 2202 82-83 0.3514942580912468 2202 84-85 0.4760777212357077 2202 86-87 0.3676250032242727 2202 88-89 0.6132161587712162 2202 90-91 0.45619898310267004 2202 92-93 0.315212296965651 2202 94-95 0.3379058245917186 2202 96-97 0.3964471708791635 2202 98-99 0.3643293014095441 2202 100-101 0.5746183459289504 1102 1 0.14945814356516252 1102 2 0.1378190500239569 1102 3 0.11015480606003791 1102 4 0.1272068204885528 1102 5 0.18185170932186168 1102 6 0.17876124864684328 1102 7 0.23042903311031182 1102 8 0.13384941335208111 1102 9 0.16877635091459098 1102 10-11 0.23263773778749908 1102 12-13 0.2282568172444428 1102 14-15 0.27392063045540027 1102 16-17 0.19462443903132254 1102 18-19 0.19542998808111633 1102 20-21 0.2349005479985351 1102 22-23 0.1563205822014595 1102 24-25 0.20692057517803164 1102 26-27 0.27948115047233557 1102 28-29 0.27059270096429344 1102 30-31 0.27502076232642025 1102 32-33 0.3257539025397236 1102 34-35 0.3577747355784666 1102 36-37 0.2958204507685238 1102 38-39 0.321353696328444 1102 40-41 0.3101276066313545 1102 42-43 0.2686065234969348 1102 44-45 0.40019059122388967 1102 46-47 0.2524056042492191 1102 48-49 0.28466988038274366 1102 50-51 0.3151824825279661 1102 52-53 0.28369870052559776 1102 54-55 0.3889468864800918 1102 56-57 0.3640049928244551 1102 58-59 0.4540984382153894 1102 60-61 0.4619542792593663 1102 62-63 0.5042205152855885 1102 64-65 0.5815620535026227 1102 66-67 0.5977061337243939 1102 68-69 0.5740078935457049 1102 70-71 0.5070528914782741 1102 72-73 0.4443261693665903 1102 74-75 0.5982862526007509 1102 76-77 0.40866135207044607 1102 78-79 0.4697304251236005 1102 80-81 0.5148302530385749 1102 82-83 0.44132900184938606 1102 84-85 0.44317253751244934 1102 86-87 0.35095293101543135 1102 88-89 0.5123893791785576 1102 90-91 0.3903629297465194 1102 92-93 0.36589673061434524 1102 94-95 0.4338430804318172 1102 96-97 0.43779535143501036 1102 98-99 0.4423711456731887 1102 100-101 0.47313906755733015 2305 1 0.30785281392572017 2305 2 0.28797197688132314 2305 3 0.3436592674639769 2305 4 0.4092492571366506 2305 5 0.34742877976856334 2305 6 0.472594173281081 2305 7 0.32765473593799044 2305 8 0.4394968712578802 2305 9 0.36135677863120463 2305 10-11 0.3595672609787499 2305 12-13 0.38513621006817544 2305 14-15 0.4406141277495337 2305 16-17 0.349837200246391 2305 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64-65 -1.0538995354237528 1213 66-67 -1.0478938597736729 1213 68-69 -1.0372287894421675 1213 70-71 -1.149803237376105 1213 72-73 -0.9867905529433614 1213 74-75 -1.160164602060437 1213 76-77 -1.1688299290951 1213 78-79 -1.2000202026561055 1213 80-81 -1.3541521397697913 1213 82-83 -1.1744835576368438 1213 84-85 -1.2803199334619855 1213 86-87 -1.1501015943360962 1213 88-89 -1.1153139432083528 1213 90-91 -1.2019409770494995 1213 92-93 -1.3171390464449217 1213 94-95 -1.1233213053272415 1213 96-97 -1.2087954135917407 1213 98-99 -1.3142276140323261 1213 100-101 -1.0846015914530298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3992.0 3 527.0 4 450.0 5 424.0 6 762.0 7 3768.0 8 15556.0 9 37735.0 10 46939.0 11 35718.0 12 23774.0 13 18456.0 14 16617.0 15 17213.0 16 17352.0 17 17760.0 18 18801.0 19 20237.0 20 22277.0 21 25089.0 22 27749.0 23 30008.0 24 33949.0 25 38320.0 26 44654.0 27 52313.0 28 63270.0 29 77388.0 30 95664.0 31 124489.0 32 169288.0 33 237980.0 34 356717.0 35 592498.0 36 1103213.0 37 1594854.0 38 333608.0 39 84.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.80780882428883 24.08984184912549 26.42486372835306 13.677485598232625 2 31.6101560447703 22.104702976734053 34.43038551949156 11.854755459004092 3 38.63977843601628 18.206634561841472 27.598482719229967 15.55510428291228 4 29.847648338614675 16.89194547945665 36.71237423730449 16.548031944624196 5 30.831487839334436 16.105896499345647 37.21446656273869 15.84814909858122 6 24.771935708451526 16.83870682077897 43.20873650583103 15.180620964938477 7 34.207725993242285 17.262935098318785 31.97144858747101 16.557890320967925 8 29.62008301618959 28.14454521549324 26.05966572007013 16.175706048247037 9 30.80026757949039 26.899931146493746 24.44048992960972 17.85931134440614 10-11 25.576203740249408 27.230254396615877 28.88998254007948 18.30355932305523 12-13 26.78153538866541 24.11120537473432 28.462439545917224 20.644819690683043 14-15 24.85850672265528 25.05823645465034 29.87758266374556 20.205674158948817 16-17 23.703877689462566 26.739537469463855 31.139059683920543 18.41752515715304 18-19 22.79682083268238 27.21582159235793 31.114039194060233 18.873318380899455 20-21 22.707210233862092 28.08437624925074 30.137053253808197 19.07136026307896 22-23 23.605177931984734 27.41568557195083 30.141176530396198 18.837959965668237 24-25 22.93358001179252 28.015562953055422 30.281024204127682 18.769832831024374 26-27 22.116081776800108 28.562218471096674 30.225038300990953 19.09666145111226 28-29 21.899840024762092 28.385326463813954 29.78065399551187 19.934179515912085 30-31 22.278800814105864 28.008646269531322 29.52519319839113 20.18735971797168 32-33 21.13157285533978 28.047960916124975 30.73845685266743 20.08200937586782 34-35 20.598192598403813 28.17073193729916 32.08224582472127 19.14882963957576 36-37 20.676636363464937 29.114362058204023 30.803638269907808 19.405363308423233 38-39 20.83490790021545 28.908689581546376 29.341765812903258 20.914636705334917 40-41 21.448116589048087 28.000888812012768 29.944957599875583 20.60603699906356 42-43 21.71003713263276 28.270512979816175 31.101865613589467 18.917584273961594 44-45 20.879395124647314 29.332210329341667 31.22539322038215 18.56300132562887 46-47 21.735732478241644 28.991678177189755 30.58301251514073 18.68957682942787 48-49 21.394531548432553 29.478856886308307 29.90796310601409 19.21864845924505 50-51 20.49021418804525 29.613258115283315 29.832749965141304 20.063777731530127 52-53 21.69037799779646 29.029677996287184 29.440106328387945 19.83983767752841 54-55 22.636456759402936 28.872752896566332 29.056065196484038 19.43472514754669 56-57 22.84119138536965 28.596379111057274 28.409187394361496 20.153242109211583 58-59 22.08361223447165 28.328089237296254 29.195440334273865 20.392858193958226 60-61 20.599456587644244 28.395398402125522 29.453839841777874 21.551305168452362 62-63 21.723474760425553 28.372326735706842 29.077394448189224 20.826804055678373 64-65 21.01056182067664 28.72452999963985 29.59514057820467 20.66976760147884 66-67 21.197212049437848 29.001418113381185 28.75024986705187 21.0511199701291 68-69 21.632042796549953 28.55227177981789 29.910258089440546 19.90542733419161 70-71 20.96998487388858 30.557375287356052 29.158514011568247 19.314125827187123 72-73 20.452324947856127 31.68593921339047 28.36012973295869 19.501606105794718 74-75 21.610241038652788 31.08040928932492 27.695192152406182 19.61415751961611 76-77 22.115251897860595 29.85440307428903 28.303041102399618 19.727303925450755 78-79 21.94719985367339 29.777540185604746 29.02543839055816 19.249821570163704 80-81 20.718118128897945 30.674271512184443 28.838950604495256 19.76865975442236 82-83 19.83500070878901 30.262348137356238 29.088342651338717 20.814308502516035 84-85 19.61391343646806 29.376138451553103 29.928112390031025 21.081835721947808 86-87 19.894825914885516 29.369359156192864 30.612521105270098 20.123293823651522 88-89 20.10523059628156 29.970613144110654 30.004856941843073 19.919299317764715 90-91 20.413781555041655 29.614980382388094 29.48726059729601 20.48397746527424 92-93 19.770602089621125 30.50816300673765 29.270504803232622 20.450730100408602 94-95 19.578648139368056 30.96897144473879 29.429597148348712 20.02278326754444 96-97 20.374286458993694 31.29176197436225 28.56607421533467 19.76787735130939 98-99 20.785082391760135 30.428545015192675 28.217609173289667 20.56876341975752 100-101 20.513820654866798 30.312085813696488 28.241944120603318 20.932149410833393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5237.0 1 3330.0 2 1199.5 3 1174.0 4 1235.0 5 1061.5 6 1248.5 7 1700.5 8 2104.5 9 2367.0 10 2486.5 11 2594.0 12 2624.5 13 2457.0 14 2188.0 15 2027.0 16 1910.0 17 1827.0 18 1893.0 19 2036.5 20 2262.5 21 2686.0 22 3177.0 23 3885.0 24 5085.0 25 6531.0 26 8227.5 27 10354.5 28 12911.5 29 15685.5 30 19193.0 31 24859.0 32 36360.0 33 56551.5 34 85569.5 35 126705.5 36 178507.5 37 264100.5 38 372863.5 39 435006.5 40 425305.5 41 364578.0 42 317663.5 43 312619.0 44 308011.0 45 299871.5 46 299943.0 47 270971.5 48 214301.5 49 171590.0 50 142744.0 51 119517.5 52 97678.5 53 72307.5 54 50222.0 55 36982.0 56 27048.5 57 19770.0 58 14899.5 59 10887.0 60 8005.5 61 6179.5 62 4632.0 63 3340.5 64 2466.5 65 1800.5 66 1332.5 67 981.0 68 751.0 69 548.0 70 384.0 71 314.5 72 253.0 73 187.5 74 152.5 75 123.0 76 91.0 77 68.0 78 56.5 79 57.5 80 48.0 81 33.0 82 24.0 83 23.5 84 21.5 85 18.0 86 14.5 87 10.5 88 6.0 89 6.5 90 9.5 91 9.5 92 7.5 93 5.0 94 5.5 95 4.5 96 2.0 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25466712711154993 2 0.2432374664277216 3 0.20770776463095258 4 0.21020800290554006 5 0.2250402434968896 6 0.2276156769075549 7 0.2047939531079372 8 0.2001882510231708 9 0.1817654426841054 10-11 0.1700350014559658 12-13 0.19748122612436936 14-15 0.1939752529047411 16-17 0.19581753373864763 18-19 0.1877904529623406 20-21 0.18912516662772186 22-23 0.15746801433896052 24-25 0.46265687350279433 26-27 0.32116782558037016 28-29 0.2746408351322203 30-31 0.27779903084748864 32-33 0.3205286669237087 34-35 0.31681590707986645 36-37 0.308732429951501 38-39 0.3340637914177159 40-41 0.3390266703988519 42-43 0.37553390896463257 44-45 0.3428052259867623 46-47 0.3690671272619402 48-49 0.3451080770291454 50-51 0.3670274591958294 52-53 0.3559173778403318 54-55 0.3310841841506324 56-57 0.3680613923168994 58-59 0.35584218270425394 60-61 0.3209704383481659 62-63 0.2973591656197311 64-65 0.30352516677811214 66-67 0.31343212595636466 68-69 0.3197579167789111 70-71 0.29609024769841785 72-73 0.3033653771139468 74-75 0.3009215351914177 76-77 0.3036567582662483 78-79 0.3061945941088747 80-81 0.32445761278377466 82-83 0.342090872194023 84-85 0.33619745340392404 86-87 0.3401076004799706 88-89 0.3346747518983482 90-91 0.30975696368056127 92-93 0.29260307326280904 94-95 0.3015230962800402 96-97 0.28951067329160884 98-99 0.3091366038079193 100-101 0.3324000990319942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5319493.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.68763592628141 #Duplication Level Percentage of deduplicated Percentage of total 1 86.9398348945234 32.765568449958145 2 5.799333562736277 4.371263438549385 3 2.0376671738823764 2.3038457576464126 4 1.1226597472231377 1.6924156728974693 5 0.7247661453092983 1.3657361308055602 6 0.4967326688071221 1.1232407984815764 7 0.35378754471836166 0.9333391326419033 8 0.30280012703612685 0.9129456756935448 9 0.2318069024513389 0.7862628730306581 >10 1.515170244316965 11.568987414657487 >50 0.2144948436528063 5.631175080680293 >100 0.2100408226986235 16.73155959468072 >500 0.03565448442699204 9.089844967284662 >1k 0.014546561955500607 8.680737817279182 >5k 6.036676846382302E-4 1.5112155185341656 >10k+ 1.0060857688464937E-4 0.5318616771789538 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 14454 0.27171762421719514 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13600 0.255663462664581 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9859 0.1853372116478018 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 9515 0.17887042994510943 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6956 0.1307643416393254 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6929 0.13025677447080014 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6688 0.1257262675221116 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 6178 0.11613888767218981 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG 5916 0.11121360625909274 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5769 0.10845018500823293 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 5759 0.1082621971680384 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 5558 0.10448364158012803 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 5552 0.10437084887601131 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012783173133229051 0.0 0.0 2 0.0013911100174396319 0.0 0.0 3 0.0015602990736147224 0.0 0.0 4 0.0017106893457703581 0.0 0.0 5 0.002049067458120539 1.8798784019454487E-5 0.0 6 0.002312250434392902 1.8798784019454487E-5 0.0 7 0.002744622466840355 1.8798784019454487E-5 0.0 8 0.003139396931248899 1.8798784019454487E-5 0.0 9 0.003722159235851988 1.8798784019454487E-5 0.0 10-11 0.006184799942400526 1.8798784019454487E-5 0.0 12-13 0.008779032137085245 1.8798784019454487E-5 0.0 14-15 0.012454194412888597 1.8798784019454487E-5 0.0 16-17 0.015790978576341767 1.8798784019454487E-5 0.0 18-19 0.020030104372728758 1.8798784019454487E-5 0.0 20-21 0.024363224089213015 1.8798784019454487E-5 0.0 22-23 0.029363700638387905 1.8798784019454487E-5 0.0 24-25 0.03476835104398107 1.8798784019454487E-5 1.8798784019454487E-5 26-27 0.04152651389897496 1.8798784019454487E-5 1.8798784019454487E-5 28-29 0.04946900014719448 1.8798784019454487E-5 1.8798784019454487E-5 30-31 0.0777329719204443 1.8798784019454487E-5 2.819817602918173E-5 32-33 0.08797830921104699 1.8798784019454487E-5 3.759756803890897E-5 34-35 0.09703932310842406 1.8798784019454487E-5 3.759756803890897E-5 36-37 0.10713427012687111 1.8798784019454487E-5 3.759756803890897E-5 38-39 0.11773678431384343 1.8798784019454487E-5 3.759756803890897E-5 40-41 0.12856488390904922 1.8798784019454487E-5 3.759756803890897E-5 42-43 0.14102847771394755 1.8798784019454487E-5 3.759756803890897E-5 44-45 0.15430981862369214 1.8798784019454487E-5 3.759756803890897E-5 46-47 0.16952743428744055 1.8798784019454487E-5 3.759756803890897E-5 48-49 0.18529021468775314 1.8798784019454487E-5 3.759756803890897E-5 50-51 0.2022749160493303 1.8798784019454487E-5 5.639635205836346E-5 52-53 0.2199833705956564 1.8798784019454487E-5 5.639635205836346E-5 54-55 0.2382745874465856 1.8798784019454487E-5 5.639635205836346E-5 56-57 0.25798511249098366 1.8798784019454487E-5 5.639635205836346E-5 58-59 0.27844758889615984 1.8798784019454487E-5 5.639635205836346E-5 60-61 0.29995339781441577 1.8798784019454487E-5 5.639635205836346E-5 62-63 0.32300070702226696 1.8798784019454487E-5 5.639635205836346E-5 64-65 0.34755191895167453 1.8798784019454487E-5 5.639635205836346E-5 66-67 0.37387021657891084 1.8798784019454487E-5 5.639635205836346E-5 68-69 0.4016548193596645 1.8798784019454487E-5 5.639635205836346E-5 70-71 0.42967440694066145 1.8798784019454487E-5 5.639635205836346E-5 72-73 0.46126576348535475 1.8798784019454487E-5 5.639635205836346E-5 74-75 0.49285712003004795 1.8798784019454487E-5 5.639635205836346E-5 76-77 0.5259147817282587 1.8798784019454487E-5 5.639635205836346E-5 78-79 0.563155172870798 1.8798784019454487E-5 5.639635205836346E-5 80-81 0.6028300065438568 1.8798784019454487E-5 5.639635205836346E-5 82-83 0.6453058590358141 1.8798784019454487E-5 5.639635205836346E-5 84-85 0.6919926391481294 1.8798784019454487E-5 7.519513607781795E-5 86-87 0.7411702581430223 1.8798784019454487E-5 7.519513607781795E-5 88-89 0.7949535792226816 1.8798784019454487E-5 7.519513607781795E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGG 1435 0.0 75.711685 8 GAGCGGC 1465 0.0 72.8645 9 CGGGAGA 2000 0.0 72.83993 4 GAGAGCG 1995 0.0 68.96915 7 ATTCGGA 365 0.0 66.30568 2 CGGAAAG 905 0.0 65.5412 5 AAGCGGC 705 0.0 60.56539 9 GGATCGG 885 0.0 58.99024 1 TCGGGAG 2545 0.0 56.495777 3 TCGGGAA 770 0.0 56.08049 3 GAACCGG 245 0.0 54.240368 1 ACCGGAA 380 0.0 53.696507 3 TCGGAGA 525 0.0 53.32782 4 AACCGGA 260 0.0 51.104374 2 GATCCGG 540 0.0 48.33922 1 AAAGCGG 985 0.0 46.72129 8 GGAAAGC 1055 0.0 45.875004 6 TCGGAAA 940 0.0 45.433456 4 CGGAGAG 1130 0.0 44.512337 5 TTGTGTT 71785 0.0 43.205856 1 >>END_MODULE