##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4617420 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.224898752983265 33.0 33.0 33.0 33.0 33.0 2 32.23116350689346 33.0 33.0 33.0 33.0 33.0 3 32.213338617669606 33.0 33.0 33.0 33.0 33.0 4 35.99620480701344 37.0 37.0 37.0 37.0 37.0 5 35.956943921064145 37.0 37.0 37.0 33.0 37.0 6 35.95373780162948 37.0 37.0 37.0 33.0 37.0 7 35.94182660446743 37.0 37.0 37.0 33.0 37.0 8 35.91842609076064 37.0 37.0 37.0 33.0 37.0 9 35.850859354358064 37.0 37.0 37.0 33.0 37.0 10-11 35.78610631045042 37.0 37.0 37.0 33.0 37.0 12-13 35.80693753221496 37.0 37.0 37.0 33.0 37.0 14-15 37.66759889721966 40.0 37.0 40.0 33.0 40.0 16-17 37.63885990444881 40.0 37.0 40.0 33.0 40.0 18-19 37.62210942474369 40.0 37.0 40.0 33.0 40.0 20-21 37.595513620160176 40.0 37.0 40.0 33.0 40.0 22-23 37.60148665271949 40.0 37.0 40.0 33.0 40.0 24-25 37.4230988300826 40.0 37.0 40.0 33.0 40.0 26-27 37.34593950734393 40.0 37.0 40.0 33.0 40.0 28-29 37.263677768104266 40.0 37.0 40.0 33.0 40.0 30-31 37.286806160150036 40.0 37.0 40.0 33.0 40.0 32-33 37.192220330834104 40.0 37.0 40.0 33.0 40.0 34-35 37.1313419615283 40.0 37.0 40.0 33.0 40.0 36-37 37.03253245318814 40.0 37.0 40.0 33.0 40.0 38-39 36.996279848919954 40.0 37.0 40.0 33.0 40.0 40-41 36.919593950734395 40.0 37.0 40.0 33.0 40.0 42-43 36.79405154826721 40.0 37.0 40.0 33.0 40.0 44-45 36.68847451607175 40.0 37.0 40.0 33.0 40.0 46-47 36.61272214786612 40.0 37.0 40.0 33.0 40.0 48-49 36.48951958019846 38.5 37.0 40.0 33.0 40.0 50-51 35.34879564778599 37.0 35.0 38.5 30.0 38.5 52-53 35.757708633825814 37.0 37.0 38.5 33.0 40.0 54-55 36.525268548236895 37.0 37.0 40.0 33.0 40.0 56-57 36.559178935422814 37.0 37.0 40.0 33.0 40.0 58-59 36.40307227845853 37.0 37.0 40.0 33.0 40.0 60-61 36.149993936007554 37.0 37.0 40.0 33.0 40.0 62-63 35.91342881955724 37.0 37.0 40.0 33.0 40.0 64-65 35.66790198855638 37.0 37.0 40.0 33.0 40.0 66-67 35.42915166911392 37.0 37.0 40.0 27.0 40.0 68-69 35.19218979430072 37.0 35.0 37.0 27.0 40.0 70-71 34.8756951934197 37.0 33.0 37.0 27.0 40.0 72-73 34.62420940698485 37.0 33.0 37.0 27.0 40.0 74-75 34.37475971429933 37.0 33.0 37.0 27.0 38.5 76-77 34.13115527718942 37.0 33.0 37.0 27.0 37.0 78-79 33.86389293155052 37.0 33.0 37.0 27.0 37.0 80-81 33.625518254782975 37.0 33.0 37.0 27.0 37.0 82-83 33.39167132294658 37.0 33.0 37.0 27.0 37.0 84-85 33.236276210524494 37.0 33.0 37.0 27.0 37.0 86-87 33.07488565042816 37.0 33.0 37.0 27.0 37.0 88-89 32.852512550298655 37.0 33.0 37.0 27.0 37.0 90-91 32.77768580722568 37.0 33.0 37.0 27.0 37.0 92-93 32.62911723429967 35.0 33.0 37.0 27.0 37.0 94-95 32.4912797622915 33.0 33.0 37.0 24.5 37.0 96-97 32.3471146224515 33.0 33.0 37.0 22.0 37.0 98-99 32.20157144032815 33.0 33.0 37.0 22.0 37.0 100-101 30.957661854455516 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2940711721937106 2306 2 0.26115470436848653 2306 3 0.21750425603897838 2306 4 0.34387941294123436 2306 5 0.34497297308190866 2306 6 0.42992109475157037 2306 7 0.3917389669067717 2306 8 0.40533658409831475 2306 9 0.4050015875912578 2306 10-11 0.43822390907117637 2306 12-13 0.38329539967041626 2306 14-15 0.5826237584683085 2306 16-17 0.5457922310307382 2306 18-19 0.5276421389542136 2306 20-21 0.5266069644447384 2306 22-23 0.5425356403661965 2306 24-25 0.5422541444671864 2306 26-27 0.4802719194505869 2306 28-29 0.4018267543045084 2306 30-31 0.3714653848413647 2306 32-33 0.45243928005749723 2306 34-35 0.3786374034768656 2306 36-37 0.3500338366208524 2306 38-39 0.4002881834641201 2306 40-41 0.31441250020706235 2306 42-43 0.28104628033325696 2306 44-45 0.3107983946452819 2306 46-47 0.2759701136423587 2306 48-49 0.2648570199916236 2306 50-51 0.375620988424302 2306 52-53 0.2796499705458402 2306 54-55 0.2662916714357664 2306 56-57 0.2699338628646899 2306 58-59 0.35812290640417643 2306 60-61 0.3393921916748752 2306 62-63 0.38250752773456753 2306 64-65 0.334492868114566 2306 66-67 0.3463688137961398 2306 68-69 0.36777790129758614 2306 70-71 0.3892220214022615 2306 72-73 0.37554197478013407 2306 74-75 0.4576776722375726 2306 76-77 0.37687057951190894 2306 78-79 0.37173208687865866 2306 80-81 0.3182831168044089 2306 82-83 0.37101667486533074 2306 84-85 0.27290436541384366 2306 86-87 0.296326464508347 2306 88-89 0.3394892024806566 2306 90-91 0.33718039880391615 2306 92-93 0.21420131494883066 2306 94-95 0.1472635093893686 2306 96-97 0.1469239382969718 2306 98-99 0.23238035525593403 2306 100-101 0.18281429211779 2307 1 0.270510112979359 2307 2 0.25714995112803507 2307 3 0.21680705458907568 2307 4 0.27001822754491656 2307 5 0.3506610390179219 2307 6 0.33530817024554693 2307 7 0.3685633107006936 2307 8 0.27243399418630077 2307 9 0.3600470524197661 2307 10-11 0.32538559161648806 2307 12-13 0.172327901357626 2307 14-15 0.3378965521880346 2307 16-17 0.3120714717507198 2307 18-19 0.29110740586147443 2307 20-21 0.32913925595528326 2307 22-23 0.31458932554265573 2307 24-25 0.4025005364662988 2307 26-27 0.28685873038600107 2307 28-29 0.22614599753939757 2307 30-31 0.1689171092681434 2307 32-33 0.16244455299368354 2307 34-35 0.18263523313937213 2307 36-37 0.14073668710253173 2307 38-39 0.09284105386176833 2307 40-41 0.12395944937850345 2307 42-43 0.16097116188689142 2307 44-45 0.21888276884216396 2307 46-47 0.11642874892061883 2307 48-49 0.10754677588849404 2307 50-51 0.10593499595267275 2307 52-53 0.2393881301353602 2307 54-55 0.15027710324004317 2307 56-57 0.21436350725111453 2307 58-59 0.18384590744185658 2307 60-61 0.21931982746389878 2307 62-63 0.23108593388324294 2307 64-65 0.20481154644626542 2307 66-67 0.22974180811917932 2307 68-69 0.27108391451525904 2307 70-71 0.21549482480930493 2307 72-73 0.036551109534237014 2307 74-75 0.1658350906566497 2307 76-77 0.068120079238291 2307 78-79 0.15614098712386237 2307 80-81 0.16738074381218127 2307 82-83 0.23959833291124255 2307 84-85 0.3465353822072714 2307 86-87 0.3707677013765789 2307 88-89 0.36244129075144116 2307 90-91 0.2675267828442358 2307 92-93 0.31255168940137423 2307 94-95 0.17636124656654317 2307 96-97 0.2550543652091548 2307 98-99 0.27211072320848473 2307 100-101 0.015258624746607552 2201 1 0.29952328528397487 2201 2 0.3302373885034413 2201 3 0.29224430038244975 2201 4 0.438529148552675 2201 5 0.40367022511311745 2201 6 0.4142911392680304 2201 7 0.36389185240374644 2201 8 0.35943330937062967 2201 9 0.4146411743724059 2201 10-11 0.3898434331717695 2201 12-13 0.41729389104102665 2201 14-15 0.5912980170558839 2201 16-17 0.5361158835716893 2201 18-19 0.5410453704340412 2201 20-21 0.4629991886243232 2201 22-23 0.43911253649637416 2201 24-25 0.5866254539834443 2201 26-27 0.5244214936033629 2201 28-29 0.4670227175495967 2201 30-31 0.3450755428978667 2201 32-33 0.543405109205402 2201 34-35 0.5333121904090987 2201 36-37 0.4333664191233453 2201 38-39 0.5285246548138574 2201 40-41 0.4877808126468821 2201 42-43 0.5018865933900685 2201 44-45 0.5131806127125031 2201 46-47 0.36544731553488674 2201 48-49 0.5368681065796039 2201 50-51 0.43931940454364593 2201 52-53 0.5166948949774195 2201 54-55 0.5989168811878756 2201 56-57 0.550002115189983 2201 58-59 0.5022009774344554 2201 60-61 0.602019844930858 2201 62-63 0.5877013970626166 2201 64-65 0.584555307207161 2201 66-67 0.4769195860586919 2201 68-69 0.510331034893099 2201 70-71 0.5607568950320214 2201 72-73 0.5254811214422688 2201 74-75 0.5450111319507513 2201 76-77 0.4386887049768191 2201 78-79 0.45091215437415144 2201 80-81 0.6437063871784758 2201 82-83 0.5955905393207814 2201 84-85 0.4524172071439949 2201 86-87 0.4579498304601728 2201 88-89 0.6083203102312496 2201 90-91 0.5593988768830158 2201 92-93 0.5351127527093951 2201 94-95 0.46911171373836424 2201 96-97 0.3866680695628659 2201 98-99 0.43965940195785436 2201 100-101 0.37441121560944524 1101 1 0.34242623818646933 1101 2 0.31333634037250135 1101 3 0.2926180674734624 1101 4 0.3775343486803351 1101 5 0.43111669773962547 1101 6 0.45594614385265686 1101 7 0.4594880496995799 1101 8 0.4187218569316613 1101 9 0.3969210364790996 1101 10-11 0.400376509580461 1101 12-13 0.4351401218983213 1101 14-15 0.5597114525342803 1101 16-17 0.5448029209933622 1101 18-19 0.5753762181080404 1101 20-21 0.5276372282040569 1101 22-23 0.4531774823674297 1101 24-25 0.45195587295719264 1101 26-27 0.5204694725698218 1101 28-29 0.5352520715483493 1101 30-31 0.5102080916949703 1101 32-33 0.5704707699990266 1101 34-35 0.4173491694464744 1101 36-37 0.4308473302475164 1101 38-39 0.39035169991033314 1101 40-41 0.4828907327832539 1101 42-43 0.5444854350761972 1101 44-45 0.5795671730547554 1101 46-47 0.3821914004846576 1101 48-49 0.45611185591469905 1101 50-51 0.41995087620763627 1101 52-53 0.4597292125271579 1101 54-55 0.46759196946086234 1101 56-57 0.5924223845429211 1101 58-59 0.5066393231200976 1101 60-61 0.5911073023141284 1101 62-63 0.646867967688614 1101 64-65 0.5331371610919291 1101 66-67 0.5320152457378171 1101 68-69 0.4880912597422622 1101 70-71 0.48489642334777017 1101 72-73 0.5164272033626389 1101 74-75 0.5976803134055757 1101 76-77 0.5873485892720822 1101 78-79 0.4065368504212401 1101 80-81 0.5463723549645394 1101 82-83 0.5518650138621908 1101 84-85 0.6362837034281696 1101 86-87 0.5111020120821692 1101 88-89 0.6816813233316239 1101 90-91 0.6354160742852315 1101 92-93 0.5944458641993222 1101 94-95 0.5994392799874362 1101 96-97 0.5185256904221376 1101 98-99 0.6011717449788421 1101 100-101 0.5099514950688153 2304 1 0.2880170892716336 2304 2 0.30155090481226665 2304 3 0.24969160554802272 2304 4 0.352985632818438 2304 5 0.38976810199729783 2304 6 0.40510066695377134 2304 7 0.3577024239308244 2304 8 0.3717989985932135 2304 9 0.37516074879158623 2304 10-11 0.4106693845996219 2304 12-13 0.4158816527195981 2304 14-15 0.5113178644060596 2304 16-17 0.5455080614652417 2304 18-19 0.46565536641190164 2304 20-21 0.49874087859386407 2304 22-23 0.5318653807246037 2304 24-25 0.6419295924791655 2304 26-27 0.6370510161177734 2304 28-29 0.5583989361625328 2304 30-31 0.49782472410488765 2304 32-33 0.4432858216031761 2304 34-35 0.546330579733187 2304 36-37 0.5393363655905645 2304 38-39 0.5415783575776416 2304 40-41 0.5348162194577029 2304 42-43 0.4598306808429058 2304 44-45 0.462308011632274 2304 46-47 0.4517309171705577 2304 48-49 0.47699776077124056 2304 50-51 0.46924694615365325 2304 52-53 0.5312338570735307 2304 54-55 0.6434483257110557 2304 56-57 0.5115266263083385 2304 58-59 0.548589669472527 2304 60-61 0.5661150547525438 2304 62-63 0.5135237213812545 2304 64-65 0.5284150182833685 2304 66-67 0.5282536559864823 2304 68-69 0.5596037575952124 2304 70-71 0.5461904004236047 2304 72-73 0.5626155974147622 2304 74-75 0.543120366594799 2304 76-77 0.64224255378263 2304 78-79 0.6399405080735647 2304 80-81 0.6006691799807626 2304 82-83 0.5943994588971222 2304 84-85 0.5180463370208415 2304 86-87 0.3885439581344201 2304 88-89 0.5658918109686439 2304 90-91 0.6166874511298204 2304 92-93 0.4789987992321585 2304 94-95 0.4207424764129044 2304 96-97 0.5744433946859218 2304 98-99 0.4312117089429677 2304 100-101 0.4860941583801264 2202 1 0.2335592054350002 2202 2 0.24780685866064545 2202 3 0.24762823756560692 2202 4 0.30731380363602767 2202 5 0.3453412629485797 2202 6 0.38236175107167725 2202 7 0.3919711836892006 2202 8 0.2825213087123828 2202 9 0.40453120376493956 2202 10-11 0.378359972760272 2202 12-13 0.34289038416371653 2202 14-15 0.4262379691958742 2202 16-17 0.34566920154593817 2202 18-19 0.3342433746669329 2202 20-21 0.38015404056454827 2202 22-23 0.3596614010209933 2202 24-25 0.3940239714429765 2202 26-27 0.45835741969689536 2202 28-29 0.4115278726718046 2202 30-31 0.3492101543266699 2202 32-33 0.3942884709941836 2202 34-35 0.40364196795374596 2202 36-37 0.39865898173676584 2202 38-39 0.41874425033503826 2202 40-41 0.3099360245657081 2202 42-43 0.374760862576359 2202 44-45 0.4528782678232943 2202 46-47 0.3869715363811892 2202 48-49 0.3062525974441428 2202 50-51 0.3491864038762529 2202 52-53 0.5000657403671838 2202 54-55 0.5311641933476068 2202 56-57 0.49907757013437504 2202 58-59 0.5052459393338609 2202 60-61 0.43541774585560233 2202 62-63 0.5117275549366624 2202 64-65 0.4999588346546773 2202 66-67 0.538241727485719 2202 68-69 0.6066513821410311 2202 70-71 0.5729897394801284 2202 72-73 0.5648465677243166 2202 74-75 0.6389265506915862 2202 76-77 0.541274894140436 2202 78-79 0.6182172830979269 2202 80-81 0.6158596849253541 2202 82-83 0.5754620212441424 2202 84-85 0.5588065988601443 2202 86-87 0.5022166283190472 2202 88-89 0.7251582952881392 2202 90-91 0.6895847115534153 2202 92-93 0.5859753728736834 2202 94-95 0.5324412358696193 2202 96-97 0.5579667466700542 2202 98-99 0.5613190290428705 2202 100-101 0.5775017890839393 1102 1 0.09669642545520674 1102 2 0.07970255319276731 1102 3 0.07960745688724558 1102 4 0.1398766117466863 1102 5 0.14827374237653856 1102 6 0.09106488950659752 1102 7 0.06760682180051703 1102 8 -0.009787251385326101 1102 9 0.018086590101447086 1102 10-11 0.10749685434236511 1102 12-13 0.10534100148785086 1102 14-15 0.17597329323951527 1102 16-17 0.09701969976588742 1102 18-19 0.14597018279783214 1102 20-21 0.17070496429087 1102 22-23 0.11143006555723645 1102 24-25 0.13924064053222907 1102 26-27 0.21392573899734657 1102 28-29 0.13874549199112352 1102 30-31 0.1737462663236542 1102 32-33 0.06169818286620199 1102 34-35 0.05619366835212958 1102 36-37 0.08395023014672631 1102 38-39 0.1130787747101678 1102 40-41 0.12563496072746005 1102 42-43 0.09360738161748117 1102 44-45 0.2533333875313062 1102 46-47 0.14669530435438816 1102 48-49 0.1626877666595803 1102 50-51 0.1787721498466368 1102 52-53 0.19217094833667403 1102 54-55 0.29882697448445583 1102 56-57 0.22466121316027454 1102 58-59 0.3679933770030317 1102 60-61 0.38381425068666175 1102 62-63 0.302266546550193 1102 64-65 0.33622909483982255 1102 66-67 0.4170727492433812 1102 68-69 0.3398068546780024 1102 70-71 0.3281910559175927 1102 72-73 0.2870389596645708 1102 74-75 0.42747649526149445 1102 76-77 0.3597874586551981 1102 78-79 0.3296969880203946 1102 80-81 0.3349104962005498 1102 82-83 0.3819499087033478 1102 84-85 0.35766064597969205 1102 86-87 0.3814547882281545 1102 88-89 0.5160096913091792 1102 90-91 0.39051172824318314 1102 92-93 0.3025605757521461 1102 94-95 0.38238642046663784 1102 96-97 0.4361883267276525 1102 98-99 0.3656707067340861 1102 100-101 0.3839007280660063 2305 1 0.24845492459422758 2305 2 0.20415622103308806 2305 3 0.23941540426343266 2305 4 0.24851647936557697 2305 5 0.27070528076439615 2305 6 0.28947067233916357 2305 7 0.22219898122937565 2305 8 0.27950418061274007 2305 9 0.27682036981022407 2305 10-11 0.2985272506931338 2305 12-13 0.2513096132164421 2305 14-15 0.32965641534041623 2305 16-17 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7208.0 15 7590.0 16 8225.0 17 8911.0 18 9838.0 19 11144.0 20 12645.0 21 14742.0 22 17120.0 23 18509.0 24 21221.0 25 24380.0 26 29492.0 27 35483.0 28 43286.0 29 52274.0 30 64625.0 31 83712.0 32 115489.0 33 165065.0 34 249652.0 35 422584.0 36 849312.0 37 1661297.0 38 624597.0 39 603.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.09366000306622 32.78910093293881 16.07055607600231 18.046682987992664 2 30.674894980220653 25.592343690594916 29.984251647084903 13.748509682099531 3 31.990283960395267 23.816777423816973 25.219182818645574 18.973755797142186 4 28.0651119157176 23.334613246603112 29.585314612304575 19.01496022537471 5 29.22039410128885 21.77829685895585 30.615552607984924 18.38575643177037 6 27.371073267598423 22.049207180025352 32.013041329020666 18.566678223355552 7 31.55799623350204 22.733877624193617 25.32104978379642 20.387076358507926 8 27.535180699881067 27.08338759104444 25.136186854875813 20.24524485419868 9 25.969630426857847 24.82003055184266 28.491784852535286 20.718554168764214 10-11 22.793008269289768 29.038476475460946 30.88590792740104 17.282607327848247 12-13 22.816359788871925 28.51008127764583 29.97790992061266 18.695649012869584 14-15 22.189572809684982 28.535045213890303 30.714682860389473 18.560699116035238 16-17 22.309092092841563 28.416560868951894 30.962129843636404 18.312217194570135 18-19 21.88145383036765 28.37198497552088 31.090164452033793 18.65639674207768 20-21 21.94836882929302 29.022059257536093 30.467268952203714 18.562302960967177 22-23 22.171837087542286 28.975175254869335 30.23877831926703 18.61420933832135 24-25 21.558085657183245 29.407683763748988 30.281412450427048 18.752818128640715 26-27 21.381255928212806 29.40626062732307 30.4696502666813 18.742833177782824 28-29 21.168631699243704 29.640297722687702 30.3535798979198 18.83749068014879 30-31 21.124632537794174 29.62014154421546 30.542372586857912 18.71285333113245 32-33 20.6156024722661 29.678774674884668 31.000986598834963 18.70463625401427 34-35 20.59847707668078 30.29492122422689 30.59744826529037 18.509153433801963 36-37 20.728855223906375 29.91702657016035 30.822291989866468 18.531826216066804 38-39 20.873994442308692 29.265165158443317 30.947608751918647 18.913231647329347 40-41 20.9970632231783 29.632204182163186 30.125368835740655 19.245363758917854 42-43 21.38247214852958 29.252520179645174 30.340750789874605 19.024256881950638 44-45 20.790839945508775 29.50754470444945 30.524484537202778 19.177130812838993 46-47 21.07261993674013 29.70806305204151 30.155864853574997 19.063452157643365 48-49 20.76853050956626 29.881349664277025 30.159243327677725 19.190876498478985 50-51 20.912497644010983 29.36778624424155 30.028256650736886 19.69145946101058 52-53 21.306247191206623 29.2708467489728 29.787681033016465 19.635225026804108 54-55 21.419441918472753 29.420242502699313 29.702815702968476 19.45749987585946 56-57 21.382045774709123 29.216638550235658 29.939498947807785 19.46181672724743 58-59 21.351741203557182 29.494580584354384 29.87023573110378 19.283442480984657 60-61 20.75668776411649 29.717037914568365 29.5934431316241 19.932831189691047 62-63 20.936180797268904 29.728937697108204 29.490183896147908 19.844697609474988 64-65 20.699199594175727 29.68720789966929 29.80527278267165 19.808319723483333 66-67 20.89435139057184 29.20072673057498 29.831575278906325 20.073346599946856 68-69 20.726168866414586 29.26132622831632 30.515934356526476 19.49657054874262 70-71 20.717743236263846 30.26485789867851 29.77357090837206 19.243827956685585 72-73 20.63429785416546 30.491910621113007 29.460716040000772 19.413075484720764 74-75 20.759124601368452 30.08572608213415 29.49793633257127 19.65721298392613 76-77 20.91719440793404 29.850650126549276 29.53380983934216 19.69834562617452 78-79 20.64253394943142 29.756844607952242 29.92255756963882 19.67806387297751 80-81 20.623802758093333 29.97949688433259 29.710873583097374 19.685826774476702 82-83 20.318297746563665 30.03625145206843 29.62488005833267 20.020570743035233 84-85 20.393824080392577 30.02072186412021 29.744361523181972 19.841092532305247 86-87 20.43967622273037 29.878525387506155 30.11258137599363 19.569217013769848 88-89 20.64551699198735 30.272355766383686 29.67678296151731 19.405344280111652 90-91 20.931491420662432 29.903303821407167 29.380297348380534 19.784907409549863 92-93 20.568408560949862 30.267572313972167 29.31971422951462 19.84430489556335 94-95 20.17970805180969 30.579870812502158 29.296207732349473 19.94421340333868 96-97 20.366752468150274 30.702648985044473 29.160622549498765 19.769975997306485 98-99 20.591536406903213 30.32198744830781 29.013100062875786 20.073376081913196 100-101 20.60204268012097 30.21312679005066 28.958174898332672 20.2266556314957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2527.0 1 1603.5 2 565.5 3 376.0 4 306.0 5 361.0 6 453.5 7 615.5 8 884.0 9 1147.5 10 1372.5 11 1520.5 12 1559.5 13 1463.0 14 1289.5 15 1168.0 16 1044.0 17 944.0 18 1035.0 19 1269.5 20 1599.0 21 2149.0 22 3046.5 23 4537.5 24 6774.5 25 9766.5 26 14274.0 27 20311.5 28 26973.5 29 34260.0 30 42191.5 31 50984.0 32 65054.0 33 87869.5 34 116451.5 35 153755.5 36 200668.5 37 256092.0 38 308158.5 39 324664.5 40 330239.5 41 316913.0 42 282286.0 43 269174.0 44 252172.0 45 236042.0 46 238252.0 47 220243.5 48 174461.0 49 131044.0 50 99376.0 51 79659.5 52 63344.0 53 47468.5 54 36801.5 55 28318.0 56 19663.0 57 12860.0 58 8399.5 59 5826.5 60 4278.0 61 3167.0 62 2196.5 63 1449.5 64 992.0 65 702.5 66 496.5 67 359.5 68 268.5 69 195.0 70 168.5 71 142.0 72 109.0 73 88.5 74 77.0 75 64.0 76 53.0 77 37.5 78 31.5 79 31.5 80 26.0 81 20.0 82 16.5 83 13.5 84 10.5 85 11.0 86 9.5 87 5.5 88 6.0 89 7.5 90 5.0 91 3.5 92 3.5 93 2.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26859155112595345 2 0.2568317372038931 3 0.2207509821502051 4 0.22386960683671836 5 0.2380117035054208 6 0.24128192800308398 7 0.21563990280286396 8 0.21180659329235804 9 0.191882046684079 10-11 0.18014388987789717 12-13 0.20997656700061937 14-15 0.2066955139450169 16-17 0.20725859895785959 18-19 0.19939706589394077 20-21 0.19915883761927655 22-23 0.16686807784433733 24-25 0.4801815732595259 26-27 0.33536043937956694 28-29 0.2815641635372134 30-31 0.28598221517644057 32-33 0.3301735601266508 34-35 0.32532236616985244 36-37 0.3179589467711406 38-39 0.34367677187693557 40-41 0.3490477366148195 42-43 0.3854425198487467 44-45 0.35327087421113956 46-47 0.3791186420122059 48-49 0.35473272953294266 50-51 0.37772175803803854 52-53 0.3662759722962174 54-55 0.3417601171216827 56-57 0.37899952787487384 58-59 0.36652502912882085 60-61 0.3310073590879756 62-63 0.3062857613125945 64-65 0.3127828960761637 66-67 0.32245279831594265 68-69 0.3297512463670188 70-71 0.3054194766774519 72-73 0.3120573826942319 74-75 0.3103139848660074 76-77 0.31337846676282427 78-79 0.31579323518328417 80-81 0.33431006925945655 82-83 0.3513867051297045 84-85 0.3465138540570275 86-87 0.3502063923143227 88-89 0.34399080005717475 90-91 0.3184570604363476 92-93 0.3018027383257317 94-95 0.3106280130462466 96-97 0.29821848564782927 98-99 0.3182296607196226 100-101 0.3415218888470185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4617420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.039164925952015 #Duplication Level Percentage of deduplicated Percentage of total 1 90.26557582518798 45.16814035852609 2 4.8124690716129255 4.816238671509647 3 1.3188546918089878 1.9798316231038664 4 0.7831358460783517 1.5674985504535845 5 0.3673134999223818 0.9190030401072363 6 0.2989204476937357 0.8974637749131754 7 0.2212033220601599 0.7748180660315775 8 0.1993574750531122 0.7980545258723236 9 0.1848744109511481 0.8325865026155457 >10 1.251886598013062 12.912942555230977 >50 0.16759079008840688 5.7466670714670505 >100 0.10095299979899874 9.795579125985562 >500 0.018475127863732267 6.615389114586059 >1k 0.009346307406607808 6.987585934071559 >5k 4.3586460372193776E-5 0.18820108552576992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 8629 0.18687925291613067 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.529130986568257E-4 0.0 0.0 2 0.0011261700256853394 0.0 0.0 3 0.001775883502042266 0.0 0.0 4 0.0025988539054277063 0.0 0.0 5 0.003140281802391812 0.0 0.0 6 0.005392621853762491 0.0 0.0 7 0.006995248428776243 0.0 0.0 8 0.008619532119668559 0.0 0.0 9 0.009724045029475334 0.0 0.0 10-11 0.012929298179502838 0.0 1.082855793928211E-5 12-13 0.01883086225641159 0.0 2.165711587856422E-5 14-15 0.026692395320330402 0.0 2.165711587856422E-5 16-17 0.0361998691910201 0.0 2.165711587856422E-5 18-19 0.048414482546530316 0.0 2.165711587856422E-5 20-21 0.06150620909512239 0.0 2.165711587856422E-5 22-23 0.07926504411554505 0.0 2.165711587856422E-5 24-25 0.09907047658649203 0.0 3.248567381784633E-5 26-27 0.11979633648227798 0.0 4.331423175712844E-5 28-29 0.14131268110763154 0.0 4.331423175712844E-5 30-31 0.16503805155259865 0.0 4.331423175712844E-5 32-33 0.18765890908775895 0.0 6.497134763569266E-5 34-35 0.21230470695756504 0.0 6.497134763569266E-5 36-37 0.242473069376405 0.0 6.497134763569266E-5 38-39 0.2757817135976368 0.0 7.579990557497477E-5 40-41 0.3096209571578934 0.0 8.662846351425688E-5 42-43 0.34890696536160887 0.0 8.662846351425688E-5 44-45 0.3914090552732911 0.0 8.662846351425688E-5 46-47 0.43485322972569096 0.0 8.662846351425688E-5 48-49 0.4842531110446959 0.0 8.662846351425688E-5 50-51 0.5370748166725141 0.0 9.745702145353899E-5 52-53 0.5909577209783818 0.0 1.082855793928211E-4 54-55 0.6508396463826119 0.0 1.2994269527138533E-4 56-57 0.7120751415292523 0.0 1.2994269527138533E-4 58-59 0.7775446028301519 0.0 1.2994269527138533E-4 60-61 0.8463384314184111 0.0 1.2994269527138533E-4 62-63 0.9188247982639656 1.082855793928211E-5 1.6242836908923167E-4 64-65 0.9935959908347085 2.165711587856422E-5 1.7325692702851376E-4 66-67 1.0708902374053042 2.165711587856422E-5 1.7325692702851376E-4 68-69 1.1543134477695336 2.165711587856422E-5 1.7325692702851376E-4 70-71 1.240887768494094 2.165711587856422E-5 1.7325692702851376E-4 72-73 1.3293354297421502 2.165711587856422E-5 1.8408548496779586E-4 74-75 1.421443143573684 2.165711587856422E-5 1.9491404290707798E-4 76-77 1.5194524214821263 2.165711587856422E-5 2.165711587856422E-4 78-79 1.621154237647864 2.165711587856422E-5 2.165711587856422E-4 80-81 1.7285627038476032 2.165711587856422E-5 2.165711587856422E-4 82-83 1.841829419892494 2.165711587856422E-5 2.165711587856422E-4 84-85 1.9630334689068787 2.165711587856422E-5 2.165711587856422E-4 86-87 2.0924130791654214 2.165711587856422E-5 2.165711587856422E-4 88-89 2.2235036015783707 2.165711587856422E-5 2.4905683260348854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 785 0.0 58.64579 2 CGTCCCG 25 0.0050365175 56.95911 1 CGAGGCG 160 0.0 56.365784 1 GAGCGGC 470 0.0 51.479305 9 CGACCGC 60 1.5006735E-6 47.465927 1 CGCGCCG 50 2.8403512E-5 47.465927 1 CGGGAGA 740 0.0 46.174534 4 AGAGCGG 585 0.0 44.60574 8 CGAGCGG 65 2.603907E-6 43.814697 1 GGACCGG 185 0.0 41.05161 1 GAGAGCG 895 0.0 38.701355 7 CCGCCGA 150 0.0 37.96614 3 GATCCGG 240 0.0 37.577194 1 GATCGGG 1515 0.0 36.03024 1 CGGTGCG 55 0.0025895243 34.52067 1 CTCGCCG 55 0.0025895243 34.52067 1 CGGAAAG 430 0.0 34.21145 5 TCGGGAA 390 0.0 34.07218 3 CGGAGCG 70 2.0629662E-4 33.904232 1 ACGCGGC 70 2.0642825E-4 33.90055 2 >>END_MODULE