FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617420
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT86290.18687925291613067Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7850.058.645792
CGTCCCG250.005036517556.959111
CGAGGCG1600.056.3657841
GAGCGGC4700.051.4793059
CGACCGC601.5006735E-647.4659271
CGCGCCG502.8403512E-547.4659271
CGGGAGA7400.046.1745344
AGAGCGG5850.044.605748
CGAGCGG652.603907E-643.8146971
GGACCGG1850.041.051611
GAGAGCG8950.038.7013557
CCGCCGA1500.037.966143
GATCCGG2400.037.5771941
GATCGGG15150.036.030241
CGGTGCG550.002589524334.520671
CTCGCCG550.002589524334.520671
CGGAAAG4300.034.211455
TCGGGAA3900.034.072183
CGGAGCG702.0629662E-433.9042321
ACGCGGC702.0642825E-433.900552
Produced by FastQC (version 0.11.2)