FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221056
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT126270.24184762622733794Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC51700.076.240019
ATCGGGA82600.072.781882
AGAGCGG56250.072.183918
TCGGGAG77250.069.7713853
CGGGAGA73900.069.594354
ATTCGGA13200.069.376132
GATCGGG90500.068.163961
TCGGAGA19400.066.520624
GAGAGCG70300.065.588117
CGGAAAG27350.065.051755
ATCCGGA15300.063.265282
GATCCGG16350.061.8189931
GGATCGG31550.061.8162351
CGGAGAG33750.060.3071175
ATCGGAG15300.059.229693
AGAGCGT128050.057.898678
GAGCGTC127300.057.6041229
GTTCGGA11550.057.513892
TCGGAAA23450.057.055234
AAGCGGC14750.056.9185339
Produced by FastQC (version 0.11.2)