##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5221056 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.139931653673127 33.0 33.0 33.0 27.0 33.0 2 30.718582600914452 33.0 33.0 33.0 27.0 33.0 3 30.57413710942767 33.0 33.0 33.0 27.0 33.0 4 33.993770417325536 37.0 37.0 37.0 27.0 37.0 5 34.01425094846713 37.0 37.0 37.0 27.0 37.0 6 34.50800642628618 37.0 37.0 37.0 27.0 37.0 7 34.043659558526095 37.0 37.0 37.0 27.0 37.0 8 34.269791398521676 37.0 37.0 37.0 27.0 37.0 9 33.76984253760159 37.0 37.0 37.0 22.0 37.0 10-11 33.76336396315228 37.0 37.0 37.0 22.0 37.0 12-13 33.773440660280215 37.0 37.0 37.0 24.5 37.0 14-15 35.349591730102105 40.0 37.0 40.0 22.0 40.0 16-17 35.26116268433053 40.0 37.0 40.0 22.0 40.0 18-19 35.289419228600494 40.0 37.0 40.0 22.0 40.0 20-21 35.26760592110102 40.0 37.0 40.0 22.0 40.0 22-23 35.920274557484156 40.0 37.0 40.0 27.0 40.0 24-25 35.3203008165398 40.0 37.0 40.0 22.0 40.0 26-27 35.17688921551502 40.0 37.0 40.0 21.0 40.0 28-29 34.868644484947104 40.0 37.0 40.0 15.0 40.0 30-31 34.873855499730325 38.5 37.0 40.0 18.5 40.0 32-33 34.72228549167065 37.0 37.0 40.0 15.0 40.0 34-35 34.70631391427328 37.0 37.0 40.0 15.0 40.0 36-37 34.51662096709938 37.0 37.0 40.0 15.0 40.0 38-39 34.46349435822944 37.0 37.0 40.0 15.0 40.0 40-41 34.495008481042916 37.0 37.0 40.0 15.0 40.0 42-43 34.1536278293127 37.0 35.0 40.0 15.0 40.0 44-45 33.985678855005574 37.0 33.0 40.0 15.0 40.0 46-47 33.9352085095429 37.0 33.0 40.0 15.0 40.0 48-49 33.820925211298245 37.0 33.0 40.0 15.0 40.0 50-51 32.82576340878167 37.0 33.0 38.5 15.0 38.5 52-53 33.25265425231984 37.0 33.0 38.5 15.0 40.0 54-55 33.903333923252305 37.0 33.0 40.0 15.0 40.0 56-57 34.08468420947793 37.0 33.0 40.0 18.5 40.0 58-59 33.665379379190725 37.0 33.0 40.0 15.0 40.0 60-61 33.40249568286569 37.0 33.0 40.0 15.0 40.0 62-63 33.14220408668285 37.0 33.0 40.0 15.0 40.0 64-65 32.91209201740031 37.0 33.0 38.5 15.0 40.0 66-67 32.77033353022837 37.0 33.0 37.0 15.0 40.0 68-69 32.726913195338255 37.0 33.0 37.0 15.0 40.0 70-71 32.359836400912 37.0 33.0 37.0 15.0 40.0 72-73 32.40804503916449 37.0 33.0 37.0 15.0 38.5 74-75 32.417312608790255 37.0 33.0 37.0 22.0 37.0 76-77 32.17625074314468 37.0 33.0 37.0 22.0 37.0 78-79 31.821557650406355 37.0 33.0 37.0 15.0 37.0 80-81 31.45962512181444 37.0 33.0 37.0 15.0 37.0 82-83 31.121223560904156 33.0 33.0 37.0 15.0 37.0 84-85 30.93867121900244 33.0 33.0 37.0 15.0 37.0 86-87 30.75132329551723 33.0 33.0 37.0 15.0 37.0 88-89 30.528981493399037 33.0 27.0 37.0 15.0 37.0 90-91 30.46162251467902 33.0 27.0 37.0 15.0 37.0 92-93 30.31353053864965 33.0 27.0 37.0 15.0 37.0 94-95 30.17128105885093 33.0 27.0 37.0 15.0 37.0 96-97 30.021858221785017 33.0 27.0 37.0 15.0 37.0 98-99 29.87338279842239 33.0 27.0 37.0 15.0 37.0 100-101 28.664742534843526 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.06374022996657658 2306 2 0.041726021404883795 2306 3 -0.11572333478196839 2306 4 0.0501785365722327 2306 5 0.05408853753087328 2306 6 -0.1535455739946343 2306 7 0.3896226754675922 2306 8 -0.140796384808894 2306 9 0.05760935675493073 2306 10-11 0.12238021768882845 2306 12-13 0.03341326997779248 2306 14-15 0.35406146622608503 2306 16-17 0.30963801567844484 2306 18-19 0.3106578570817504 2306 20-21 0.29114702205914966 2306 22-23 0.12341562281957863 2306 24-25 0.3845022144786654 2306 26-27 0.18861489820050537 2306 28-29 0.18441091456497105 2306 30-31 0.19917544267240572 2306 32-33 0.15931656842573005 2306 34-35 0.0932299009686659 2306 36-37 0.22705961316444956 2306 38-39 0.2324025845536184 2306 40-41 0.2191824738949819 2306 42-43 0.27751338613384036 2306 44-45 0.3318439399873938 2306 46-47 0.2841150132684547 2306 48-49 0.3186136556835848 2306 50-51 0.3925385684591518 2306 52-53 0.30918642904682514 2306 54-55 0.3983147062319503 2306 56-57 0.36588559919178465 2306 58-59 0.49198857426860343 2306 60-61 0.44239875832478504 2306 62-63 0.4348720905530499 2306 64-65 0.4153022036568004 2306 66-67 0.37136291636299745 2306 68-69 0.31221416288504145 2306 70-71 0.40454685503024024 2306 72-73 0.1415155166494344 2306 74-75 0.17349792621552496 2306 76-77 0.19021531059946994 2306 78-79 0.08321014971795293 2306 80-81 0.10739547648446646 2306 82-83 0.2377419351394785 2306 84-85 0.23034402374253204 2306 86-87 0.11671971254687108 2306 88-89 0.34345614901469546 2306 90-91 0.27655378596207925 2306 92-93 0.1608890915652097 2306 94-95 -0.021316343228718893 2306 96-97 0.07401117648581135 2306 98-99 0.20878300998901977 2306 100-101 0.1346956316932335 2307 1 0.15101787578065284 2307 2 0.17739841675490808 2307 3 0.16679177541910306 2307 4 0.3289215616268635 2307 5 0.4114070005503194 2307 6 0.10613217672585051 2307 7 0.5834047279190031 2307 8 0.1513728063800457 2307 9 0.4094780694354796 2307 10-11 0.4053183028717555 2307 12-13 0.2707938933335896 2307 14-15 0.5726355524891744 2307 16-17 0.5761817532473543 2307 18-19 0.515726230162187 2307 20-21 0.395788622234754 2307 22-23 0.2489579408026259 2307 24-25 0.5612864981350683 2307 26-27 0.35927483666219473 2307 28-29 0.5049474205296534 2307 30-31 0.4953509940366203 2307 32-33 0.5235150735991354 2307 34-35 0.5377205950106827 2307 36-37 0.5278723686831697 2307 38-39 0.40025098343002696 2307 40-41 0.3364049780540128 2307 42-43 0.4870350482006316 2307 44-45 0.5420773088976034 2307 46-47 0.49688330138905457 2307 48-49 0.3850216249820164 2307 50-51 0.3903066756550828 2307 52-53 0.4163775420266589 2307 54-55 0.4153330795067731 2307 56-57 0.4883521079406208 2307 58-59 0.5382199431387349 2307 60-61 0.5360748876012948 2307 62-63 0.47489496182469537 2307 64-65 0.41173263452967035 2307 66-67 0.4532714055582048 2307 68-69 0.3685747418798897 2307 70-71 0.3476907517530492 2307 72-73 0.10603209039657457 2307 74-75 0.2201686878605713 2307 76-77 0.19109885102925972 2307 78-79 0.279269281285476 2307 80-81 0.1841100817113066 2307 82-83 0.3284749731519021 2307 84-85 0.40718449871616613 2307 86-87 0.45731912048955436 2307 88-89 0.5741768303202086 2307 90-91 0.4090620329873609 2307 92-93 0.43464942109334714 2307 94-95 0.25200976707313316 2307 96-97 0.3742558285168194 2307 98-99 0.3507913576443116 2307 100-101 0.2547157397516209 2201 1 0.421175073694684 2201 2 0.3576525212589168 2201 3 0.42406045700891326 2201 4 0.5862943754864176 2201 5 0.6293487386026726 2201 6 0.5175093431768119 2201 7 0.7894989286737584 2201 8 0.32393303684572317 2201 9 0.5155198890237429 2201 10-11 0.5198566397854734 2201 12-13 0.500014364775538 2201 14-15 0.767904579468194 2201 16-17 0.686762748879012 2201 18-19 0.7294432796447765 2201 20-21 0.6257173133957963 2201 22-23 0.4919585539687077 2201 24-25 0.7795902934355396 2201 26-27 0.5805781339263163 2201 28-29 0.6332736604010307 2201 30-31 0.6705788263355643 2201 32-33 0.8091980893567197 2201 34-35 0.6658563839486575 2201 36-37 0.7056963479162874 2201 38-39 0.656949830099272 2201 40-41 0.5194683627021774 2201 42-43 0.6771104066278539 2201 44-45 0.8141464050690246 2201 46-47 0.6821611578451794 2201 48-49 0.7874654111694852 2201 50-51 0.7123779054497419 2201 52-53 0.7251098461994303 2201 54-55 0.8838607620511212 2201 56-57 0.758185911577975 2201 58-59 0.8294882690292056 2201 60-61 0.8948094972327283 2201 62-63 0.9322922908720699 2201 64-65 0.9178477992067187 2201 66-67 0.7572683262673223 2201 68-69 0.7507636560317721 2201 70-71 0.8683446542931463 2201 72-73 0.5766296738749475 2201 74-75 0.6049509136620586 2201 76-77 0.4712143702600784 2201 78-79 0.5191971905953316 2201 80-81 0.7632444492838992 2201 82-83 0.6532915603112706 2201 84-85 0.7251522984751944 2201 86-87 0.7819332568747583 2201 88-89 0.8661865115282588 2201 90-91 0.8794836569868174 2201 92-93 0.7861352963643071 2201 94-95 0.8398747491034442 2201 96-97 0.8300861043728247 2201 98-99 0.7664038664616157 2201 100-101 0.8039501126797468 1101 1 0.1316875111672715 1101 2 0.05345020906601761 1101 3 0.08479473220559797 1101 4 0.237667189301213 1101 5 0.21800788671837523 1101 6 0.2590605805628883 1101 7 0.4865295863786443 1101 8 0.22000990463935466 1101 9 0.30495130037428453 1101 10-11 0.23698881672392247 1101 12-13 0.25534350412642937 1101 14-15 0.4413895173142137 1101 16-17 0.3984526856928383 1101 18-19 0.3522255110354777 1101 20-21 0.3296718169636321 1101 22-23 0.174836575864056 1101 24-25 0.42972032071322985 1101 26-27 0.27745703947626765 1101 28-29 0.26328661888847904 1101 30-31 0.3831313185237448 1101 32-33 0.4695103799514442 1101 34-35 0.2839595338438201 1101 36-37 0.41716620876555766 1101 38-39 0.3345470759534166 1101 40-41 0.2304475224742859 1101 42-43 0.41314662986985695 1101 44-45 0.5137239178551312 1101 46-47 0.33884932844926396 1101 48-49 0.3462158770671877 1101 50-51 0.329491066903401 1101 52-53 0.4248702887869342 1101 54-55 0.43894218701668564 1101 56-57 0.5246270939662594 1101 58-59 0.5419074643443622 1101 60-61 0.5715941871287598 1101 62-63 0.5345216993978212 1101 64-65 0.5172214033787768 1101 66-67 0.48139518593801967 1101 68-69 0.26634850025060786 1101 70-71 0.433432271333011 1101 72-73 0.3366303555423755 1101 74-75 0.3724158537310771 1101 76-77 0.39737308578921215 1101 78-79 0.27098781105038583 1101 80-81 0.3830914793421343 1101 82-83 0.41334252934969484 1101 84-85 0.4439086711102149 1101 86-87 0.3466734957714479 1101 88-89 0.5346323197264162 1101 90-91 0.3755752732993578 1101 92-93 0.3936087797890835 1101 94-95 0.4066753304369719 1101 96-97 0.4238691979000606 1101 98-99 0.3997114199912275 1101 100-101 0.41373498273074105 2304 1 0.17558748907504196 2304 2 0.1039830913630162 2304 3 0.1479438913992297 2304 4 0.29389765589518646 2304 5 0.25333189128458855 2304 6 0.28753410355778897 2304 7 0.5334772446678642 2304 8 0.22753827875459365 2304 9 0.3345726302682692 2304 10-11 0.3217572359195273 2304 12-13 0.33673271407255356 2304 14-15 0.5180899098984924 2304 16-17 0.44509048556866304 2304 18-19 0.43633109065396525 2304 20-21 0.40264794557618444 2304 22-23 0.34670807850356056 2304 24-25 0.5651285200860698 2304 26-27 0.44364092041035263 2304 28-29 0.40562543688298547 2304 30-31 0.5620668803360971 2304 32-33 0.4403020976491234 2304 34-35 0.40630030363363545 2304 36-37 0.5313195462846281 2304 38-39 0.4278711797092285 2304 40-41 0.2707225714473296 2304 42-43 0.4583112024288667 2304 44-45 0.5889753714845014 2304 46-47 0.464247741212084 2304 48-49 0.4192181847524452 2304 50-51 0.43461211529964316 2304 52-53 0.442311223344916 2304 54-55 0.5268487498338814 2304 56-57 0.5057792524089919 2304 58-59 0.638444322078989 2304 60-61 0.5682224680028654 2304 62-63 0.4983438207433508 2304 64-65 0.5434074571758956 2304 66-67 0.562454394128693 2304 68-69 0.4971919274344643 2304 70-71 0.5484816603352698 2304 72-73 0.38412028423494604 2304 74-75 0.40109568236287174 2304 76-77 0.4381605901105772 2304 78-79 0.35049067336838746 2304 80-81 0.3732823989562384 2304 82-83 0.6019833278928601 2304 84-85 0.5790246102240921 2304 86-87 0.4425654029619821 2304 88-89 0.44173318958428 2304 90-91 0.6798312196939271 2304 92-93 0.6098930065632651 2304 94-95 0.48756888327310577 2304 96-97 0.6668034298155199 2304 98-99 0.6228575453212102 2304 100-101 0.5403327606219612 2202 1 0.2066663332389247 2202 2 0.12839650772222555 2202 3 0.1583205589083967 2202 4 0.24729906948834213 2202 5 0.24292996721392512 2202 6 0.2801449923066883 2202 7 0.5507509352764615 2202 8 0.20590926003469434 2202 9 0.30470605496767433 2202 10-11 0.2501123212360099 2202 12-13 0.24245086872861776 2202 14-15 0.4039334781307531 2202 16-17 0.3193589209689165 2202 18-19 0.34399819460941927 2202 20-21 0.3146573115203424 2202 22-23 0.15469963248817464 2202 24-25 0.37140661455408264 2202 26-27 0.3800637488023426 2202 28-29 0.46287153055016717 2202 30-31 0.3742536044918552 2202 32-33 0.3544682955945646 2202 34-35 0.36227115756167194 2202 36-37 0.41501586894771236 2202 38-39 0.3802877717584181 2202 40-41 0.292591827077473 2202 42-43 0.3165180778179675 2202 44-45 0.4499356960073442 2202 46-47 0.360565707863465 2202 48-49 0.39363395623992403 2202 50-51 0.29077820982222136 2202 52-53 0.32044679319286473 2202 54-55 0.42439585378196654 2202 56-57 0.44609428745570057 2202 58-59 0.3606620352982972 2202 60-61 0.4523173419716997 2202 62-63 0.487530855352027 2202 64-65 0.3466292311709722 2202 66-67 0.49700498772241275 2202 68-69 0.525867019837527 2202 70-71 0.4233954746235966 2202 72-73 0.4426155407024268 2202 74-75 0.3567797268343327 2202 76-77 0.3677372311464424 2202 78-79 0.32687680231732585 2202 80-81 0.43657333721537483 2202 82-83 0.41291715607044566 2202 84-85 0.38180483223479555 2202 86-87 0.4481028810261023 2202 88-89 0.5637280735635635 2202 90-91 0.5115544241874552 2202 92-93 0.47276584733465654 2202 94-95 0.4926905728891313 2202 96-97 0.3884050765064586 2202 98-99 0.3515957606925717 2202 100-101 0.4810674555604919 1102 1 0.08124143810536211 1102 2 0.008308968256780958 1102 3 0.043355465583978514 1102 4 0.08019662858323784 1102 5 0.10901693010133329 1102 6 0.14711233477347463 1102 7 0.19636625802943541 1102 8 -0.014906705947900889 1102 9 0.1473552427909297 1102 10-11 0.16280811462167577 1102 12-13 0.1676040070177578 1102 14-15 0.08029332557113378 1102 16-17 0.07772161190810323 1102 18-19 0.12999549682380263 1102 20-21 0.1910492995258508 1102 22-23 0.14848512175507267 1102 24-25 0.18954090878109042 1102 26-27 0.2531080808206596 1102 28-29 0.23794278360343668 1102 30-31 0.2656605002117587 1102 32-33 0.2670600330291606 1102 34-35 0.3271637777462928 1102 36-37 0.3173105118266406 1102 38-39 0.2168990127990611 1102 40-41 0.11288698169554578 1102 42-43 0.110288202709512 1102 44-45 0.29994915181877957 1102 46-47 0.2524362800387294 1102 48-49 0.24803227231135594 1102 50-51 0.1240149529720469 1102 52-53 0.27040904022970835 1102 54-55 0.4584148728393629 1102 56-57 0.40124055142874226 1102 58-59 0.4017001250053269 1102 60-61 0.4636189163365003 1102 62-63 0.46792131964375727 1102 64-65 0.5555047767675205 1102 66-67 0.5049149394104688 1102 68-69 0.4345364841045054 1102 70-71 0.5321744242266959 1102 72-73 0.4355171644284397 1102 74-75 0.558928717526463 1102 76-77 0.41442436505512603 1102 78-79 0.4010804605550611 1102 80-81 0.46762963758533616 1102 82-83 0.49861581596226046 1102 84-85 0.5649060063414915 1102 86-87 0.4804499457942235 1102 88-89 0.5522843155436767 1102 90-91 0.4841777304894812 1102 92-93 0.5309431897003449 1102 94-95 0.5575991762072832 1102 96-97 0.55284681371446 1102 98-99 0.5929897932145032 1102 100-101 0.49016468216437303 2305 1 0.18237845042246903 2305 2 0.03125555436028549 2305 3 0.05359888725431716 2305 4 0.038756075047921 2305 5 0.006085029299448763 2305 6 0.30650817524367113 2305 7 0.126743231515988 2305 8 0.2979510713577227 2305 9 0.07191071946787275 2305 10-11 0.23422923889235392 2305 12-13 0.15590937702753394 2305 14-15 0.095144069303565 2305 16-17 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-0.5387264276702268 1213 78-79 -0.46594003123847116 1213 80-81 -0.8599689602819538 1213 82-83 -1.107926359469321 1213 84-85 -1.1754265678602493 1213 86-87 -1.0757404669369777 1213 88-89 -0.976971997266137 1213 90-91 -1.126869656931781 1213 92-93 -1.050798109041338 1213 94-95 -1.0358499623727049 1213 96-97 -1.0050375650707863 1213 98-99 -1.1266102388324306 1213 100-101 -1.0554048291436722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3872.0 3 620.0 4 667.0 5 530.0 6 978.0 7 9753.0 8 59576.0 9 102062.0 10 95587.0 11 69259.0 12 45451.0 13 32251.0 14 24060.0 15 19756.0 16 17792.0 17 17269.0 18 17419.0 19 18305.0 20 19896.0 21 21925.0 22 23878.0 23 25731.0 24 28793.0 25 33311.0 26 39599.0 27 46252.0 28 54684.0 29 65700.0 30 82400.0 31 107096.0 32 143417.0 33 204275.0 34 309806.0 35 514675.0 36 959750.0 37 1543842.0 38 459629.0 39 1190.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.505995273832376 23.785343042728687 21.60952392291116 15.099137760527782 2 31.97593219727181 26.00074189474234 29.229136034388283 12.794189873597572 3 35.47970785312197 20.630047029635985 28.065117130721966 15.825127986520076 4 29.07602306405879 18.584260186782714 32.61799255318271 19.72172419597579 5 33.959138353659085 17.25857356845158 30.784796314700213 17.997491763189117 6 31.168175220427898 17.45861499733254 34.8932334025651 16.47997637967446 7 35.62301883113244 19.04854760985339 27.27963490833939 18.048798650674776 8 28.77870774623357 29.580807938323844 24.127048647835334 17.513435667607254 9 31.910577100751773 25.705591291344938 24.06072587603959 18.323105731863702 10-11 27.050978852015334 28.704278837635478 27.793397793598285 16.451344516750904 12-13 28.544294997466285 24.239724975169025 27.504392619114082 19.71158740825061 14-15 25.729666691262594 24.559031620225298 28.916876859507383 20.794424829004722 16-17 27.266579207354564 24.892713293380446 29.544337111892904 18.296370387372082 18-19 26.918716111964137 24.964458669825003 29.746229577467627 18.370595640743236 20-21 25.611928034338664 27.129577106942953 28.698212986303222 18.56028187241516 22-23 28.38584601920849 25.16075894354558 27.662566255723952 18.790828781521977 24-25 24.744950287001885 28.35769113093944 27.940686918756242 18.956671663302433 26-27 23.08377250973195 30.14681849388836 27.83422737843635 18.93518161794334 28-29 24.339526795068405 29.104359942268193 27.37541135833957 19.180701904323836 30-31 26.720875796751777 26.471686652982342 27.79065384661553 19.016783703650354 32-33 23.449287645362396 26.31461539651329 31.230030038223394 19.00606691990092 34-35 21.136674869121766 28.59133761180308 29.121536752298503 21.150450766776647 36-37 23.63452733923148 29.686757644704585 27.82801765716535 18.85069735889858 38-39 25.033590501805204 27.06023345783085 27.557711095549987 20.34846494481396 40-41 23.30050844387732 26.320289375334205 27.237275571008468 23.141926609780015 42-43 25.898429164367737 26.18561467523055 28.191885714066007 19.72407044633571 44-45 23.701275863977543 27.794704822407606 28.927017766926706 19.577001546688148 46-47 24.34706902653438 28.452302297385856 27.71461135557703 19.486017320502732 48-49 21.805678635516177 28.457598769386177 29.08078536193772 20.655937233159925 50-51 20.741883005477938 27.11000143979181 30.18640786553075 21.961707689199507 52-53 25.29952011815315 26.454267646330138 27.527692624980805 20.718519610535914 54-55 26.3744548784065 26.373263487054526 27.655911573396573 19.596370061142395 56-57 27.02037070605927 26.192449742191993 27.1207334999703 19.666446051778436 58-59 25.688840367517358 26.437886904947817 27.077058804026144 20.796213923508684 60-61 22.85293249904434 26.501603464609502 26.80326482129975 23.84219921504641 62-63 24.888395922991023 26.46160087857895 27.15970385419462 21.4902993442354 64-65 23.186177324911245 27.445191110701277 28.605264573439687 20.763366990947794 66-67 21.736520428739496 27.636736677492713 27.778901188124117 22.847841705643667 68-69 21.339849558472622 26.7484374720797 31.382510331830993 20.529202637616685 70-71 21.07504960139753 29.63442062772697 29.129200487038325 20.161329283837173 72-73 20.650061239448792 32.705237161544105 26.56563542399781 20.07906617500929 74-75 20.863228364661225 32.90269311981324 26.153214130692977 20.08086438483256 76-77 20.759616159522352 32.65035834838439 26.21796330479501 20.372062187298255 78-79 20.73168763699007 32.330761488530825 26.61539498959985 20.322155884879255 80-81 20.546720065822154 32.20969781491439 26.726183808204212 20.51739831105924 82-83 20.208595057268415 32.137133636937904 26.867553419548727 20.78671788624495 84-85 20.37548545580696 31.792977497834013 26.86271911749686 20.968817928862173 86-87 20.226912069291057 31.587956892952562 27.3827280663277 20.802402971428684 88-89 20.34214776471999 31.59060527205569 27.39618782960677 20.671059133617554 90-91 20.65947797138854 31.084603861930482 27.161570047320744 21.094348119360237 92-93 20.502405340385824 31.3527436862216 26.92979487183427 21.215056101558304 94-95 20.438607823823023 31.30484939095054 26.8839998866572 21.37254289856924 96-97 20.69281858149935 31.474401620489424 26.712605248304317 21.120174549706917 98-99 20.943724950584393 31.115208171852665 26.705435154631918 21.23563172293102 100-101 21.00086011686521 31.176344326533872 26.288657218448165 21.534138338152754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4168.0 1 2664.0 2 984.5 3 893.5 4 928.5 5 838.5 6 902.5 7 1115.5 8 1360.5 9 1642.0 10 1876.5 11 2035.0 12 2185.0 13 2197.5 14 2022.5 15 1929.5 16 1893.5 17 1849.0 18 1847.0 19 1911.5 20 2086.5 21 2433.0 22 2924.0 23 3720.0 24 4954.5 25 6801.5 26 10278.5 27 15068.5 28 19907.0 29 24902.0 30 30553.5 31 35911.5 32 46432.0 33 66329.5 34 95459.5 35 128269.0 36 168023.5 37 217752.0 38 256097.5 39 266088.5 40 266973.0 41 265504.5 42 265075.5 43 273093.0 44 271850.0 45 261420.0 46 250140.5 47 229694.0 48 202694.5 49 183199.5 50 167566.0 51 156268.5 52 146833.0 53 131357.5 54 113102.5 55 96428.0 56 82165.0 57 71036.0 58 62045.5 59 53171.5 60 44021.0 61 36383.5 62 30067.0 63 24543.0 64 20310.0 65 16354.5 66 12920.0 67 10669.0 68 9250.5 69 7218.0 70 5111.0 71 3964.0 72 3023.0 73 2270.0 74 1740.0 75 1430.5 76 1170.5 77 879.0 78 660.5 79 498.5 80 362.5 81 257.5 82 203.0 83 187.0 84 162.5 85 121.5 86 98.5 87 81.0 88 57.0 89 47.0 90 47.5 91 40.5 92 33.5 93 27.5 94 19.5 95 18.0 96 18.0 97 11.5 98 5.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2576873337501073 2 0.2445865357506221 3 0.2094786954976158 4 0.2115472425501661 5 0.22637182976010983 6 0.22951295676583433 7 0.20585873815565278 8 0.20082144301842383 9 0.18205129383787494 10-11 0.17054978916142632 12-13 0.1977856586866718 14-15 0.19472114453474546 16-17 0.1963587442846811 18-19 0.18903264014023216 20-21 0.18969342600424127 22-23 0.15714253974674855 24-25 0.4640057490285491 26-27 0.3219080584464139 28-29 0.27237784846590424 30-31 0.2752029474497113 32-33 0.31642066279312075 34-35 0.3124559476090661 36-37 0.30486169847632355 38-39 0.3295118841858812 40-41 0.3339075466725505 42-43 0.370921131663786 44-45 0.3386288137878621 46-47 0.3641600473160985 48-49 0.3409080461883573 50-51 0.3622830323980436 52-53 0.3508294107552189 54-55 0.32668678520207406 56-57 0.36255117738633713 58-59 0.3506666084408978 60-61 0.31688033991590975 62-63 0.2945189632135721 64-65 0.3006288383039753 66-67 0.3097170380857819 68-69 0.3162004008384511 70-71 0.29314950845193005 72-73 0.30012127814756245 74-75 0.2978420457470673 76-77 0.30102147917969085 78-79 0.3034443606810576 80-81 0.3204141077973498 82-83 0.33665603280255946 84-85 0.33159000784515624 86-87 0.3351525055467706 88-89 0.3298662186346976 90-91 0.30528306917221343 92-93 0.2899796516260312 94-95 0.2989529321271406 96-97 0.28680979480013236 98-99 0.3058672421824244 100-101 0.32774978854852355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5221056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.48667310860822 #Duplication Level Percentage of deduplicated Percentage of total 1 91.59492918356685 55.402725399325284 2 4.360552120919638 5.275105814222289 3 1.2002891553024588 2.1780449331776195 4 0.7491578816718988 1.8125627158170219 5 0.4194770361438448 1.268638518090029 6 0.27464700126162356 0.9967490031342803 7 0.17950686931027135 0.760044132730403 8 0.11789695986916832 0.570495589768407 9 0.14202811559001546 0.7731727379932399 >10 0.7594311911645266 9.207618653807648 >50 0.1023349593858909 4.34521041384953 >100 0.08759455169874228 11.38378330113803 >500 0.010208750918432636 4.057773815753448 >1k 0.0019144894515423592 1.7257036623415947 >5k 0.0 0.0 >10k+ 3.1733745145559495E-5 0.24237130885121438 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 12627 0.24184762622733794 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0031602802191740523 0.0 0.0 2 0.0034475784209171477 0.0 0.0 3 0.003811489476458402 0.0 0.0 4 0.004252013385797815 0.0 0.0 5 0.0047308437220363085 0.0 0.0 6 0.005611891540715135 0.0 0.0 7 0.00656955221319212 0.0 0.0 8 0.007469753245320487 0.0 0.0 9 0.008542333198494711 0.0 1.915321344953971E-5 10-11 0.012171867147182486 0.0 1.915321344953971E-5 12-13 0.01581097770259503 0.0 1.915321344953971E-5 14-15 0.020149180548915777 0.0 1.915321344953971E-5 16-17 0.02384575074467694 0.0 1.915321344953971E-5 18-19 0.02836590911876831 0.0 1.915321344953971E-5 20-21 0.032684958751639515 0.0 1.915321344953971E-5 22-23 0.03754029836109783 0.0 1.915321344953971E-5 24-25 0.04299896419421665 0.0 1.915321344953971E-5 26-27 0.048716198408904246 0.0 1.915321344953971E-5 28-29 0.0553719400826193 0.0 1.915321344953971E-5 30-31 0.08492534843525908 0.0 1.915321344953971E-5 32-33 0.09367836698169872 0.0 1.915321344953971E-5 34-35 0.1020100148322485 0.0 1.915321344953971E-5 36-37 0.11053319481729368 0.0 1.915321344953971E-5 38-39 0.12039709974380661 0.0 1.915321344953971E-5 40-41 0.1308547542872553 0.0 3.830642689907942E-5 42-43 0.14166674327952047 0.0 3.830642689907942E-5 44-45 0.1535417356182351 0.0 3.830642689907942E-5 46-47 0.16727458966155506 0.0 3.830642689907942E-5 48-49 0.18246308792704005 0.0 3.830642689907942E-5 50-51 0.1974409008445801 0.0 3.830642689907942E-5 52-53 0.21403716029860625 0.0 3.830642689907942E-5 54-55 0.23265408377155888 0.0 5.745964034861913E-5 56-57 0.25126143063778666 0.0 5.745964034861913E-5 58-59 0.2716021433211978 0.0 5.745964034861913E-5 60-61 0.29423166501182907 0.0 6.703624707338898E-5 62-63 0.31881481447431326 0.0 7.661285379815885E-5 64-65 0.34614645006680644 0.0 1.053426739724684E-4 66-67 0.3744836293654004 0.0 1.1491928069723826E-4 68-69 0.4060960081638657 0.0 1.244958874220081E-4 70-71 0.44045687309233994 0.0 1.3407249414677797E-4 72-73 0.4769533213204379 0.0 1.3407249414677797E-4 74-75 0.5159109574768017 0.0 1.3407249414677797E-4 76-77 0.5596664736022751 0.0 1.532257075963177E-4 78-79 0.6060842863972346 0.0 1.532257075963177E-4 80-81 0.657750079677368 0.0 1.532257075963177E-4 82-83 0.7160716146312163 0.0 1.532257075963177E-4 84-85 0.7803306457544221 0.0 1.6280231432108753E-4 86-87 0.8509676969563245 0.0 1.723789210458574E-4 88-89 0.927302829159465 0.0 1.723789210458574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5170 0.0 76.24001 9 ATCGGGA 8260 0.0 72.78188 2 AGAGCGG 5625 0.0 72.18391 8 TCGGGAG 7725 0.0 69.771385 3 CGGGAGA 7390 0.0 69.59435 4 ATTCGGA 1320 0.0 69.37613 2 GATCGGG 9050 0.0 68.16396 1 TCGGAGA 1940 0.0 66.52062 4 GAGAGCG 7030 0.0 65.58811 7 CGGAAAG 2735 0.0 65.05175 5 ATCCGGA 1530 0.0 63.26528 2 GATCCGG 1635 0.0 61.818993 1 GGATCGG 3155 0.0 61.816235 1 CGGAGAG 3375 0.0 60.307117 5 ATCGGAG 1530 0.0 59.22969 3 AGAGCGT 12805 0.0 57.89867 8 GAGCGTC 12730 0.0 57.604122 9 GTTCGGA 1155 0.0 57.51389 2 TCGGAAA 2345 0.0 57.05523 4 AAGCGGC 1475 0.0 56.918533 9 >>END_MODULE