##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3489055 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.962775593964555 33.0 33.0 33.0 33.0 33.0 2 31.86791036541413 33.0 33.0 33.0 33.0 33.0 3 31.82454991394518 33.0 33.0 33.0 33.0 33.0 4 35.50520785714183 37.0 37.0 37.0 33.0 37.0 5 35.487298709822575 37.0 37.0 37.0 33.0 37.0 6 35.59283244316871 37.0 37.0 37.0 33.0 37.0 7 35.46588947437057 37.0 37.0 37.0 33.0 37.0 8 35.51416587012816 37.0 37.0 37.0 33.0 37.0 9 35.33059897307437 37.0 37.0 37.0 33.0 37.0 10-11 35.28088493875849 37.0 37.0 37.0 33.0 37.0 12-13 35.28944184026907 37.0 37.0 37.0 33.0 37.0 14-15 37.03543724590183 40.0 37.0 40.0 33.0 40.0 16-17 36.99476792426603 40.0 37.0 40.0 33.0 40.0 18-19 36.97921070318468 40.0 37.0 40.0 33.0 40.0 20-21 36.949739542655536 40.0 37.0 40.0 33.0 40.0 22-23 37.1116829628653 40.0 37.0 40.0 33.0 40.0 24-25 36.81377077747413 40.0 37.0 40.0 33.0 40.0 26-27 36.7288992291609 40.0 37.0 40.0 33.0 40.0 28-29 36.56918793197585 40.0 37.0 40.0 33.0 40.0 30-31 36.56378360329659 40.0 37.0 40.0 33.0 40.0 32-33 36.412508831187814 40.0 37.0 40.0 33.0 40.0 34-35 36.42919214515105 40.0 37.0 40.0 33.0 40.0 36-37 36.25131117164963 40.0 37.0 40.0 33.0 40.0 38-39 36.190127556028784 40.0 37.0 40.0 33.0 40.0 40-41 36.280332353602915 40.0 37.0 40.0 33.0 40.0 42-43 36.05170726744061 38.5 37.0 40.0 27.0 40.0 44-45 35.85200505581024 37.0 37.0 40.0 27.0 40.0 46-47 35.75699508892809 37.0 37.0 40.0 27.0 40.0 48-49 35.63900282454705 37.0 37.0 40.0 27.0 40.0 50-51 34.57638629944211 37.0 35.0 38.5 24.5 38.5 52-53 34.98520587952898 37.0 35.0 38.5 27.0 40.0 54-55 35.686405201408405 37.0 37.0 40.0 27.0 40.0 56-57 35.76647458982447 37.0 37.0 40.0 27.0 40.0 58-59 35.5596620001691 37.0 37.0 40.0 27.0 40.0 60-61 35.3594327117228 37.0 37.0 40.0 27.0 40.0 62-63 35.121090811122215 37.0 35.0 40.0 27.0 40.0 64-65 34.91835224724173 37.0 33.0 40.0 27.0 40.0 66-67 34.735321025320616 37.0 33.0 40.0 27.0 40.0 68-69 34.56705956197308 37.0 33.0 40.0 27.0 40.0 70-71 34.2356673941798 37.0 33.0 38.5 27.0 40.0 72-73 34.09540964530511 37.0 33.0 37.0 27.0 40.0 74-75 33.907663106485856 37.0 33.0 37.0 27.0 40.0 76-77 33.604940592796616 37.0 33.0 37.0 27.0 40.0 78-79 33.21211459836546 37.0 33.0 37.0 22.0 37.0 80-81 32.83381746633401 37.0 33.0 37.0 22.0 37.0 82-83 32.49041302014442 37.0 33.0 37.0 22.0 37.0 84-85 32.280294377703996 37.0 33.0 37.0 22.0 37.0 86-87 32.06684030489631 37.0 33.0 37.0 22.0 37.0 88-89 31.793636959004658 33.0 33.0 37.0 15.0 37.0 90-91 31.674287020410972 33.0 33.0 37.0 15.0 37.0 92-93 31.489543587017113 33.0 33.0 37.0 15.0 37.0 94-95 31.31381362575253 33.0 33.0 37.0 15.0 37.0 96-97 31.14079070120706 33.0 33.0 37.0 15.0 37.0 98-99 30.97273344788202 33.0 33.0 37.0 15.0 37.0 100-101 29.642049924693076 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2894157293619344 2306 2 0.2685340931333364 2306 3 0.3092133511402828 2306 4 0.4500745765289693 2306 5 0.4321824715831184 2306 6 0.4157161347269138 2306 7 0.4604743705813803 2306 8 0.39561070136949184 2306 9 0.3979899380063614 2306 10-11 0.4232605410605501 2306 12-13 0.4011258720768538 2306 14-15 0.6142012800802661 2306 16-17 0.6360081433387705 2306 18-19 0.6505133241782914 2306 20-21 0.5920165639072863 2306 22-23 0.5778916344857876 2306 24-25 0.4942208077010264 2306 26-27 0.5391513209081396 2306 28-29 0.5866303093229419 2306 30-31 0.463033782904013 2306 32-33 0.6007118144182968 2306 34-35 0.33612888267020935 2306 36-37 0.48259945205033006 2306 38-39 0.49759833052149105 2306 40-41 0.37848526144077965 2306 42-43 0.5073692570390804 2306 44-45 0.5813681728986779 2306 46-47 0.4002626035688337 2306 48-49 0.5136186604431785 2306 50-51 0.3834131444054094 2306 52-53 0.38632551834763973 2306 54-55 0.4500577121272116 2306 56-57 0.45297802152877154 2306 58-59 0.5502589914473148 2306 60-61 0.49643139141173975 2306 62-63 0.5337094126628656 2306 64-65 0.5496244961306544 2306 66-67 0.39559068018810706 2306 68-69 0.36319326542792396 2306 70-71 0.5087592556706682 2306 72-73 0.4756503453889067 2306 74-75 0.5664317177559468 2306 76-77 0.45684532462784233 2306 78-79 0.3933089214241363 2306 80-81 0.44737978077594676 2306 82-83 0.563020257778966 2306 84-85 0.5416063610380348 2306 86-87 0.71532878172399 2306 88-89 0.6358757439077785 2306 90-91 0.4522794778765906 2306 92-93 0.4452937127348875 2306 94-95 0.46692421097635517 2306 96-97 0.4457587227484687 2306 98-99 0.5900327623998898 2306 100-101 0.47843008204960213 2307 1 0.1143693446245706 2307 2 0.07243465624279821 2307 3 0.09693631626744903 2307 4 0.13782775728586216 2307 5 0.21920872921506174 2307 6 0.2057849314706175 2307 7 0.19924052408177317 2307 8 0.08159039946291102 2307 9 0.10766368412015481 2307 10-11 0.20623116197925384 2307 12-13 0.1795276900089462 2307 14-15 0.3584519449489747 2307 16-17 0.24746086586689842 2307 18-19 0.33473327398538544 2307 20-21 0.18401916095314164 2307 22-23 0.12523702909941647 2307 24-25 0.20987425190823927 2307 26-27 0.19139913901504002 2307 28-29 0.13212505904512994 2307 30-31 0.06420894615580863 2307 32-33 0.1517797246703836 2307 34-35 0.06711663410295898 2307 36-37 0.11313471818510124 2307 38-39 -0.1042128592501399 2307 40-41 -0.023931239789071412 2307 42-43 -0.005271863686097333 2307 44-45 -0.03873870552710912 2307 46-47 -0.016364483120057116 2307 48-49 0.1300664510813263 2307 50-51 -0.0418321390846188 2307 52-53 -0.05005509870046154 2307 54-55 -0.10833037142774771 2307 56-57 -0.025818347601337166 2307 58-59 0.05490470694594052 2307 60-61 0.0505256092389601 2307 62-63 -0.07661334520006591 2307 64-65 -0.10741162528123738 2307 66-67 0.046879089622372305 2307 68-69 -0.09034783157424187 2307 70-71 -0.1337870978521991 2307 72-73 -0.2951793358486796 2307 74-75 -0.14114366524589173 2307 76-77 -0.09776855208739477 2307 78-79 0.05380810360440336 2307 80-81 -0.0653060391039304 2307 82-83 0.06070214463267121 2307 84-85 -0.05062073064517136 2307 86-87 0.024497102758807898 2307 88-89 0.1633944413893893 2307 90-91 0.09783540800606616 2307 92-93 0.005726720188910406 2307 94-95 -0.16065516748065178 2307 96-97 -0.01466254805599121 2307 98-99 -0.02457836743991848 2307 100-101 -0.14980954537420033 2201 1 0.33591356036690456 2201 2 0.30495562060338344 2201 3 0.2933171052897485 2201 4 0.4198165127651805 2201 5 0.40080249708241666 2201 6 0.4268302907235295 2201 7 0.533056990634087 2201 8 0.3875080667013364 2201 9 0.5243475034454832 2201 10-11 0.4877932450743643 2201 12-13 0.4863689802700506 2201 14-15 0.656489394920392 2201 16-17 0.6206036141039846 2201 18-19 0.5286783854776687 2201 20-21 0.5444303482982988 2201 22-23 0.47334451909369335 2201 24-25 0.6954159900443173 2201 26-27 0.6309781841837037 2201 28-29 0.5490020840117822 2201 30-31 0.4145479493957609 2201 32-33 0.5125959053450515 2201 34-35 0.5019379605752263 2201 36-37 0.46293103036349237 2201 38-39 0.4386035120096281 2201 40-41 0.423892278411806 2201 42-43 0.5680221614711414 2201 44-45 0.6308887006097237 2201 46-47 0.4935966851665867 2201 48-49 0.5212990601912963 2201 50-51 0.5115381201111049 2201 52-53 0.49885403985489774 2201 54-55 0.5927240406172345 2201 56-57 0.6944820621566166 2201 58-59 0.5604404892977541 2201 60-61 0.6233217308322523 2201 62-63 0.6901214556385042 2201 64-65 0.6228504292260979 2201 66-67 0.5377908010765395 2201 68-69 0.5018515204364391 2201 70-71 0.6811185625251639 2201 72-73 0.5159084285881406 2201 74-75 0.5384439562521095 2201 76-77 0.41165063983179095 2201 78-79 0.49334045542449445 2201 80-81 0.8347373190054626 2201 82-83 0.61094537886175 2201 84-85 0.5994331096445151 2201 86-87 0.7507668759625474 2201 88-89 0.7560664444387477 2201 90-91 0.6454002069387919 2201 92-93 0.6889256910293895 2201 94-95 0.57532634576501 2201 96-97 0.6243121041262079 2201 98-99 0.5892014114813726 2201 100-101 0.3985035382926547 1101 1 0.15904531783986897 1101 2 0.12424489344629208 1101 3 0.10979297271961386 1101 4 0.17138353983040844 1101 5 0.2170160155616756 1101 6 0.292449328247379 1101 7 0.27028580838548777 1101 8 0.22936023262600003 1101 9 0.2069282397892067 1101 10-11 0.19647063536479692 1101 12-13 0.21497429084880793 1101 14-15 0.39390989523838726 1101 16-17 0.2817123696689592 1101 18-19 0.3218732126983568 1101 20-21 0.2926186388784586 1101 22-23 0.3193024019288302 1101 24-25 0.3790434722010545 1101 26-27 0.3361522884872912 1101 28-29 0.28727543925169385 1101 30-31 0.3061655912585266 1101 32-33 0.44775861469421585 1101 34-35 0.39058275599604997 1101 36-37 0.4349767338473427 1101 38-39 0.3529214047380549 1101 40-41 0.2473535179001729 1101 42-43 0.36506521496558975 1101 44-45 0.41565126155948207 1101 46-47 0.2854378094687604 1101 48-49 0.32289700379951825 1101 50-51 0.2155983455368755 1101 52-53 0.3827345959993451 1101 54-55 0.45089219127796554 1101 56-57 0.6615758187815928 1101 58-59 0.4928935844853797 1101 60-61 0.44083771910162994 1101 62-63 0.5222755436888207 1101 64-65 0.4782687742193872 1101 66-67 0.37406783096842133 1101 68-69 0.2612819493813845 1101 70-71 0.3407821905373112 1101 72-73 0.22211382043323624 1101 74-75 0.4881855822856096 1101 76-77 0.3625401529404044 1101 78-79 0.2620489389238898 1101 80-81 0.4480658823578736 1101 82-83 0.5655665187105328 1101 84-85 0.5194802794558413 1101 86-87 0.46506003545657393 1101 88-89 0.5538722532155127 1101 90-91 0.48040038360474924 1101 92-93 0.6476300228787295 1101 94-95 0.4133784268900236 1101 96-97 0.43665398809200795 1101 98-99 0.3612484399599225 1101 100-101 0.5334432068673145 2304 1 0.2055038993407905 2304 2 0.09071705038478939 2304 3 0.06825132885753504 2304 4 0.16240765367091825 2304 5 0.23116800680471528 2304 6 0.26090924046312125 2304 7 0.32501199234607725 2304 8 0.14728893206688554 2304 9 0.21797800208490514 2304 10-11 0.16889975333696583 2304 12-13 0.22905867319322937 2304 14-15 0.4201120995031147 2304 16-17 0.32285600638381595 2304 18-19 0.34891274780669335 2304 20-21 0.2760568733203641 2304 22-23 0.30343295685838 2304 24-25 0.5186688598790639 2304 26-27 0.5285173121504059 2304 28-29 0.4606290820835355 2304 30-31 0.4106267054102446 2304 32-33 0.37537658149073394 2304 34-35 0.3885670986336507 2304 36-37 0.4813816574922143 2304 38-39 0.31087671847553366 2304 40-41 0.3384414845893815 2304 42-43 0.4185390245660514 2304 44-45 0.44590262178613216 2304 46-47 0.29871879956351677 2304 48-49 0.3562159836563623 2304 50-51 0.3843536442501616 2304 52-53 0.46579856689532306 2304 54-55 0.6063872923157092 2304 56-57 0.6169385190595449 2304 58-59 0.6307182145144594 2304 60-61 0.5274565229607049 2304 62-63 0.43398757807277377 2304 64-65 0.43910779528546584 2304 66-67 0.5835172477075261 2304 68-69 0.4959260099933047 2304 70-71 0.5636794733034876 2304 72-73 0.5049359285430839 2304 74-75 0.5261722590399529 2304 76-77 0.640936695228163 2304 78-79 0.6860907437595429 2304 80-81 0.5855606507169142 2304 82-83 0.6258289861133832 2304 84-85 0.7532553510890736 2304 86-87 0.5887090647859523 2304 88-89 0.4544026611225398 2304 90-91 0.6744364470497963 2304 92-93 0.673028969038441 2304 94-95 0.5589205322096618 2304 96-97 0.5583791149928352 2304 98-99 0.5175501527186874 2304 100-101 0.6109405776323698 2202 1 0.254011347326049 2202 2 0.19744134047946105 2202 3 0.21082695868335932 2202 4 0.18945492619044302 2202 5 0.2781559879835669 2202 6 0.3772333447890901 2202 7 0.31624584716478665 2202 8 0.3011014258491542 2202 9 0.36081576178924735 2202 10-11 0.31274004593743854 2202 12-13 0.2630876623692018 2202 14-15 0.3712256774614531 2202 16-17 0.37234025445889785 2202 18-19 0.3770458462532673 2202 20-21 0.30026036293514835 2202 22-23 0.3380303455572218 2202 24-25 0.38184525100196254 2202 26-27 0.3894151186810717 2202 28-29 0.34891231975813497 2202 30-31 0.3660353226745272 2202 32-33 0.3735537384416503 2202 34-35 0.30440863281125274 2202 36-37 0.42022868987172046 2202 38-39 0.3372650341562533 2202 40-41 0.34108863930436684 2202 42-43 0.3154775347326009 2202 44-45 0.4137024471477986 2202 46-47 0.23867154231129462 2202 48-49 0.3745917313852303 2202 50-51 0.38722057436823576 2202 52-53 0.26572094985660755 2202 54-55 0.4634352740264589 2202 56-57 0.40611105297021055 2202 58-59 0.49853808437786284 2202 60-61 0.3881231036051034 2202 62-63 0.40496719030277717 2202 64-65 0.39655613760024266 2202 66-67 0.38766633644605974 2202 68-69 0.3969974674107988 2202 70-71 0.4127878992831242 2202 72-73 0.30348874752892385 2202 74-75 0.38334293329364755 2202 76-77 0.3872364654305116 2202 78-79 0.4928025860708729 2202 80-81 0.28555048071786615 2202 82-83 0.42183204105304384 2202 84-85 0.4376308466610581 2202 86-87 0.2875640041550369 2202 88-89 0.5552955771887689 2202 90-91 0.5308499119315364 2202 92-93 0.42540254339171923 2202 94-95 0.3772469197373489 2202 96-97 0.48036715773342564 2202 98-99 0.5269573402731709 2202 100-101 0.46678902888680085 1102 1 0.10750174865133744 1102 2 0.06124479611604983 1102 3 0.06836290070787143 1102 4 0.04236117789083238 1102 5 0.12065300661614486 1102 6 0.12986610772864537 1102 7 0.1546411724827621 1102 8 0.0878473667053683 1102 9 0.015474972842682178 1102 10-11 0.11302334980418038 1102 12-13 0.12927197729626982 1102 14-15 0.2124612925594036 1102 16-17 0.011452384154736706 1102 18-19 0.09180442423237167 1102 20-21 0.1477500915892378 1102 22-23 0.2123021453309164 1102 24-25 0.1929626451215043 1102 26-27 0.16658379011599322 1102 28-29 0.0817460069650977 1102 30-31 0.1329556777307559 1102 32-33 0.06658740321076095 1102 34-35 0.1490743141774118 1102 36-37 0.008109099187159075 1102 38-39 0.012569612787139306 1102 40-41 0.04260854210001952 1102 42-43 -0.05592323054400339 1102 44-45 0.11772079327460716 1102 46-47 -0.09952289818532023 1102 48-49 0.02973154617672691 1102 50-51 -0.07666668092557671 1102 52-53 -0.19269438389121518 1102 54-55 -0.04657880241326495 1102 56-57 0.11202808840912581 1102 58-59 0.12374171011975932 1102 60-61 0.12370519449414275 1102 62-63 0.1502708187478845 1102 64-65 0.2682084294680749 1102 66-67 0.3088767919238222 1102 68-69 0.29358743741671844 1102 70-71 0.23371430838022178 1102 72-73 0.17022611931039933 1102 74-75 0.3763547751145211 1102 76-77 0.4198700683389376 1102 78-79 0.3249253510024488 1102 80-81 0.41936053892747793 1102 82-83 0.2994416149347501 1102 84-85 0.30139050557800573 1102 86-87 0.16368274930186288 1102 88-89 0.381138878674836 1102 90-91 0.28605223711388206 1102 92-93 0.3035344294107247 1102 94-95 0.24317292103187782 1102 96-97 0.20088543941234605 1102 98-99 0.2674773227730469 1102 100-101 0.31860691760183 2305 1 0.2075630562572428 2305 2 0.22307582442899943 2305 3 0.22423855816608906 2305 4 0.3304461358682147 2305 5 0.29695470127724377 2305 6 0.4075160518808616 2305 7 0.2844945318040928 2305 8 0.23397279580795072 2305 9 0.2803189896773972 2305 10-11 0.2848499044405628 2305 12-13 0.30448789917196706 2305 14-15 0.35137429722423263 2305 16-17 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256.0 6 400.0 7 1607.0 8 6552.0 9 15994.0 10 23420.0 11 19535.0 12 13186.0 13 9988.0 14 8685.0 15 8376.0 16 8412.0 17 9281.0 18 10012.0 19 11350.0 20 12969.0 21 14625.0 22 17082.0 23 19053.0 24 21320.0 25 24667.0 26 29595.0 27 36712.0 28 45474.0 29 53313.0 30 61254.0 31 76360.0 32 100702.0 33 139462.0 34 204989.0 35 325735.0 36 564381.0 37 1005650.0 38 581825.0 39 3612.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.46031350553234 29.00240846413785 16.218495774626838 18.318782255702967 2 31.916657376108976 26.65893922136315 27.12600524791997 14.298398154607906 3 35.19766859715141 23.470397462709418 23.513999245729366 17.81793469440981 4 30.33862200422588 23.654795593961516 27.121500406157978 18.885081995654627 5 33.31126992170936 21.55264939055571 26.92103941651469 18.215041271220233 6 31.4413681951511 21.900287003812352 28.44515181898362 18.213192982052924 7 33.907160862042424 22.720927667572766 23.10038051558023 20.271530954804582 8 29.35191009170623 28.345467966005796 22.73435637621095 19.568265566077024 9 29.8435330157742 24.283912570540515 25.096456577597724 20.77609783608757 10-11 25.971856519616292 29.729474423324813 27.83044485499801 16.46822420206088 12-13 26.43464110385662 28.1792414274222 27.36497111144746 18.02114635727372 14-15 24.912911174208237 28.177993634858332 28.214769179250553 18.694326011682875 16-17 25.468636197583393 28.0391922598848 28.279017390445247 18.213154152086556 18-19 24.95249382642305 28.049075018831598 28.65468719442625 18.3437439603191 20-21 24.312448271722715 29.04861898063776 28.111163314032932 18.527769433606593 22-23 25.354216645415605 28.62255948323391 27.526934876443132 18.496288994907353 24-25 23.342459323592067 30.06479861029622 27.86297048463256 18.72977158147916 26-27 23.193664432309326 30.09636205215801 27.805081797032933 18.90489171849973 28-29 23.448079650430138 29.812742746870786 27.73789732175572 19.001280280943355 30-31 23.986042001596743 28.56187898431478 28.35968515758502 19.092393856503453 32-33 22.816230647158363 29.501725739342273 28.546944580974333 19.135099032525034 34-35 24.219611166919 28.60279535231134 28.029237519845022 19.148355960924636 36-37 22.8787368073049 29.091278896359405 28.65715343234667 19.37283086398902 38-39 22.710423318170562 29.932450482330186 28.009801020647302 19.34732517885195 40-41 24.07747245692312 28.44918059139594 27.975351816499405 19.497995135181533 42-43 23.75998471888883 28.403635937882516 28.25334195197209 19.583037391256568 44-45 22.576438157774216 29.129344295272386 28.62981944779354 19.664398099159854 46-47 23.08648085240355 29.230573848994734 27.869032830676655 19.813912467925057 48-49 21.774788892266393 28.969261080513842 28.791318894541178 20.464631132678583 50-51 21.413687805192357 28.45045701188783 29.34464759919183 20.79120758372798 52-53 23.44732028324408 28.403947814982484 28.038388085801557 20.11034381597188 54-55 23.283256434247974 28.327354316824998 28.55865541714423 19.830733831782794 56-57 23.46023838816303 28.2494460554517 28.39437917795578 19.89593637842949 58-59 22.976922440850046 28.51501892870368 28.005910501206188 20.502148129240084 60-61 21.81298090718337 28.350529691535115 27.987094537130158 21.849394864151357 62-63 22.758761440463598 28.438506070919377 28.255070597433658 20.54766189118337 64-65 21.76266952407851 29.04685623610731 28.94368469581428 20.246789543999903 66-67 21.395397644413876 28.96235331491931 28.285332534098647 21.356916506568172 68-69 21.27382889157011 28.45298351444012 30.000363703850763 20.272823890139012 70-71 21.210089507123 29.772997012596647 28.793029258041457 20.223884222238894 72-73 21.156660544625026 30.739543396689612 27.89963569588181 20.204160362803552 74-75 21.019496715142196 30.846287282083257 28.02085969864885 20.113356304125702 76-77 21.161577622223234 30.77845512884179 27.868747368782653 20.191219880152318 78-79 21.12753715263953 30.880681777347203 27.84837382188869 20.143407248124582 80-81 21.12924887627737 30.694570276907452 27.885472156147202 20.290708690667977 82-83 21.262528072818867 30.619851579101905 27.734715654104047 20.382904693975178 84-85 21.21991102619797 30.44292366976028 27.90637520972078 20.430790094320972 86-87 21.47446280448676 30.059626642234537 27.8806782733652 20.585232279913505 88-89 21.520443133450158 30.173427175884044 27.770475483365242 20.535654207300556 90-91 21.572276788813706 29.89117490253981 27.83933977898051 20.697208529665975 92-93 21.69572343270523 29.848260388174708 27.738849548670153 20.717166630449903 94-95 21.603752404973196 29.783650867888817 27.904487529784582 20.7081091973534 96-97 21.747772619258914 29.60472300286637 27.828825637351734 20.81867874052299 98-99 21.699723514094888 29.70585550718669 27.804693288946098 20.789727689772324 100-101 21.901867799070413 29.575109929734683 27.72367236050742 20.799349910687482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1785.0 1 1148.0 2 394.0 3 249.5 4 202.5 5 208.5 6 274.0 7 369.0 8 491.5 9 675.0 10 844.0 11 973.5 12 1094.0 13 1135.5 14 1125.0 15 1062.5 16 940.0 17 934.0 18 1151.0 19 1479.5 20 2042.5 21 2938.0 22 4165.0 23 5931.0 24 8505.0 25 11888.5 26 16055.5 27 21032.0 28 26929.5 29 33989.0 30 41824.5 31 50475.5 32 60471.0 33 71492.5 34 83284.0 35 96035.0 36 109489.5 37 123184.5 38 135567.0 39 145620.5 40 154502.5 41 161362.5 42 164885.5 43 166204.0 44 166019.5 45 163856.0 46 159778.0 47 155303.5 48 149109.0 49 140283.5 50 130147.5 51 119021.0 52 105698.0 53 90690.5 54 76292.5 55 63456.0 56 52466.0 57 42767.5 58 34522.0 59 27932.0 60 22385.5 61 17525.0 62 13620.0 63 10406.5 64 7911.5 65 6091.5 66 4620.5 67 3503.0 68 2680.0 69 2048.5 70 1577.0 71 1225.5 72 954.5 73 727.5 74 556.5 75 446.0 76 376.0 77 293.0 78 221.0 79 184.5 80 156.5 81 131.0 82 106.0 83 86.0 84 70.5 85 55.5 86 46.5 87 38.5 88 30.5 89 26.5 90 20.0 91 14.5 92 15.0 93 14.5 94 11.0 95 8.0 96 5.0 97 2.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2649427996979125 2 0.2527618509883049 3 0.21627632697105661 4 0.21928573782872438 5 0.23441877528442515 6 0.23688362608213398 7 0.21389745934070975 8 0.2087957914105682 9 0.19085397048771086 10-11 0.17837208069233645 12-13 0.20710478911911676 14-15 0.20410970878934268 16-17 0.2047259214887699 18-19 0.19707341959355756 20-21 0.19824852288083736 22-23 0.16335368745978496 24-25 0.46925313587776635 26-27 0.3308918890645175 28-29 0.2679808716113675 30-31 0.27150618147320693 32-33 0.3147127230725798 34-35 0.31105843845969755 36-37 0.30287570703241995 38-39 0.32839837721102133 40-41 0.33357169778063117 42-43 0.3688104658711313 44-45 0.3367817360288101 46-47 0.36188882089849544 48-49 0.33825778040185667 50-51 0.35995419963285186 52-53 0.3495215753262703 54-55 0.3249303894607566 56-57 0.36181716825902716 58-59 0.34944992268680203 60-61 0.31507098626992125 62-63 0.2916262426359 64-65 0.2975734117117672 66-67 0.30746147595838985 68-69 0.3138528913989605 70-71 0.28987791823287395 72-73 0.2971578264028512 74-75 0.2949079335235472 76-77 0.2977740391022784 78-79 0.30094108576677636 80-81 0.3183096855738875 82-83 0.3357499380204668 84-85 0.33001772686300446 86-87 0.33437420734267587 88-89 0.328369716155234 90-91 0.30333428392501693 92-93 0.2866965410404823 94-95 0.2959540620597841 96-97 0.2841027154917306 98-99 0.3031623175902931 100-101 0.3261341538038237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3489055.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.24495564714451 #Duplication Level Percentage of deduplicated Percentage of total 1 97.84924266430889 93.1964677766878 2 1.5869807522020603 3.023038227127145 3 0.2440962674986453 0.6974681451462598 4 0.1145516140493338 0.43641853597750446 5 0.0655332590149272 0.3120856174144786 6 0.030617304818661456 0.17496863036931082 7 0.01689012491221272 0.11260894386969016 8 0.018083775970311727 0.13779107521801903 9 0.005851965249415301 0.0501633153566272 >10 0.06639591413245423 1.1714614903364713 >50 0.001417632374373312 0.09454697060421079 >100 3.0874763311540716E-4 0.04460433452474603 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.9977835581604574E-5 0.5483769373677471 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 19206 0.5504642374511151 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0034106656386901323 0.0 2.8661055787312038E-5 2 0.004327819423884118 0.0 2.8661055787312038E-5 3 0.006792670221592953 0.0 2.8661055787312038E-5 4 0.009171537851939852 0.0 2.8661055787312038E-5 5 0.011894338151734496 0.0 5.7322111574624076E-5 6 0.02138114761733478 0.0 5.7322111574624076E-5 7 0.030638668636636567 0.0 5.7322111574624076E-5 8 0.03906501903810631 2.8661055787312038E-5 5.7322111574624076E-5 9 0.043507482685139674 2.8661055787312038E-5 8.598316736193611E-5 10-11 0.05267902053707953 2.8661055787312038E-5 8.598316736193611E-5 12-13 0.06402879862885509 4.2991583680968056E-5 1.1464422314924815E-4 14-15 0.07557920411114184 5.7322111574624076E-5 1.2897475104290418E-4 16-17 0.08833337393649571 5.7322111574624076E-5 1.433052789365602E-4 18-19 0.10489946418156205 7.16526394682801E-5 1.8629686261752824E-4 20-21 0.12526314431844726 8.598316736193611E-5 2.4361897419215232E-4 22-23 0.1463290203221216 8.598316736193611E-5 2.722800299794644E-4 24-25 0.1670223026005609 8.598316736193611E-5 3.4393266944774444E-4 26-27 0.18755794907217 8.598316736193611E-5 3.4393266944774444E-4 28-29 0.20952664833314466 8.598316736193611E-5 3.7259372523505647E-4 30-31 0.239549104270354 8.598316736193611E-5 4.012547810223685E-4 32-33 0.2737560743525109 8.598316736193611E-5 4.7290742049064865E-4 34-35 0.31134504901757065 1.1464422314924815E-4 4.8723794838430464E-4 36-37 0.3569304582472904 1.1464422314924815E-4 4.8723794838430464E-4 38-39 0.4116730748010564 1.1464422314924815E-4 4.8723794838430464E-4 40-41 0.47306505629747886 1.1464422314924815E-4 5.158990041716167E-4 42-43 0.5405761732044924 1.1464422314924815E-4 5.302295320652727E-4 44-45 0.6138338317968619 1.1464422314924815E-4 5.732211157462408E-4 46-47 0.690487825500028 1.1464422314924815E-4 6.305432273208648E-4 48-49 0.7731893019743168 1.1464422314924815E-4 6.305432273208648E-4 50-51 0.8634859582322434 1.1464422314924815E-4 6.592042831081768E-4 52-53 0.9571072969614982 1.1464422314924815E-4 6.592042831081768E-4 54-55 1.0560739225950866 1.1464422314924815E-4 6.878653388954889E-4 56-57 1.1594830118757085 1.1464422314924815E-4 6.878653388954889E-4 58-59 1.267449209026513 1.1464422314924815E-4 7.021958667891449E-4 60-61 1.380345107772735 1.1464422314924815E-4 7.16526394682801E-4 62-63 1.4915213431717183 1.1464422314924815E-4 7.16526394682801E-4 64-65 1.6084297897281643 1.1464422314924815E-4 7.16526394682801E-4 66-67 1.730869820051561 1.1464422314924815E-4 7.59517978363769E-4 68-69 1.8568064991810103 1.1464422314924815E-4 8.02509562044737E-4 70-71 1.987343277764323 1.1464422314924815E-4 8.02509562044737E-4 72-73 2.1227381053035854 1.1464422314924815E-4 8.02509562044737E-4 74-75 2.264968594648121 1.1464422314924815E-4 8.16840089938393E-4 76-77 2.411842175030202 1.1464422314924815E-4 8.741622015130172E-4 78-79 2.563229871698784 1.1464422314924815E-4 9.601453688749533E-4 80-81 2.724075716777179 1.1464422314924815E-4 9.888064246622653E-4 82-83 2.8864549283401955 1.1464422314924815E-4 0.0010031369525559213 84-85 3.0569308881631274 1.1464422314924815E-4 0.0010461285362368894 86-87 3.2351739941044206 1.1464422314924815E-4 0.0010747895920242014 88-89 3.425813006673727 1.1464422314924815E-4 0.0011034506478115134 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1680 0.0 72.877266 2 GAGCGGC 1050 0.0 67.30894 9 GATCGGG 1910 0.0 65.347496 1 AAGCGGC 485 0.0 57.70136 9 TCGGGAG 1725 0.0 56.664337 3 GATCCGG 395 0.0 52.86426 1 AGAGCGG 1430 0.0 50.419178 8 GTTCGGA 315 0.0 49.71472 2 GAGAGCG 1915 0.0 47.067024 7 CGGGAGA 2060 0.0 46.067486 4 GGTTCGG 430 0.0 45.250355 1 TCGGGAA 820 0.0 42.820286 3 AGCGGCG 1070 0.0 41.672638 10-11 TCGGAGA 595 0.0 41.468475 4 TTCGGAG 435 0.0 41.45014 3 GGATCGG 885 0.0 41.29087 1 AAAGCGG 680 0.0 41.155823 8 TCGGAAA 685 0.0 38.79084 4 CGCCGGA 720 0.0 37.933216 58-59 CGTCGGG 495 0.0 37.85396 12-13 >>END_MODULE