FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489055
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT192060.5504642374511151Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA16800.072.8772662
GAGCGGC10500.067.308949
GATCGGG19100.065.3474961
AAGCGGC4850.057.701369
TCGGGAG17250.056.6643373
GATCCGG3950.052.864261
AGAGCGG14300.050.4191788
GTTCGGA3150.049.714722
GAGAGCG19150.047.0670247
CGGGAGA20600.046.0674864
GGTTCGG4300.045.2503551
TCGGGAA8200.042.8202863
AGCGGCG10700.041.67263810-11
TCGGAGA5950.041.4684754
TTCGGAG4350.041.450143
GGATCGG8850.041.290871
AAAGCGG6800.041.1558238
TCGGAAA6850.038.790844
CGCCGGA7200.037.93321658-59
CGTCGGG4950.037.8539612-13
Produced by FastQC (version 0.11.2)