##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34211-1.34200000035897.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3800862 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.040899932699475 33.0 33.0 33.0 33.0 33.0 2 31.992046277923272 33.0 33.0 33.0 33.0 33.0 3 31.961490051467273 33.0 33.0 33.0 33.0 33.0 4 35.683875657679756 37.0 37.0 37.0 33.0 37.0 5 35.646493611186095 37.0 37.0 37.0 33.0 37.0 6 35.64520416684426 37.0 37.0 37.0 33.0 37.0 7 35.609127087486996 37.0 37.0 37.0 33.0 37.0 8 35.56591583698645 37.0 37.0 37.0 33.0 37.0 9 35.45302644505378 37.0 37.0 37.0 33.0 37.0 10-11 35.36371302088842 37.0 37.0 37.0 33.0 37.0 12-13 35.3695178883106 37.0 37.0 37.0 33.0 37.0 14-15 37.056755283406765 40.0 37.0 40.0 33.0 40.0 16-17 37.01848304410947 40.0 37.0 40.0 33.0 40.0 18-19 36.99180409601822 40.0 37.0 40.0 33.0 40.0 20-21 36.95243868364597 40.0 37.0 40.0 33.0 40.0 22-23 36.97653137630358 40.0 37.0 40.0 33.0 40.0 24-25 36.75673702439079 40.0 37.0 40.0 33.0 40.0 26-27 36.659206385288385 40.0 37.0 40.0 33.0 40.0 28-29 36.53721642616859 40.0 37.0 40.0 33.0 40.0 30-31 36.53011040653409 40.0 37.0 40.0 33.0 40.0 32-33 36.41176672554805 40.0 37.0 40.0 33.0 40.0 34-35 36.32082682823001 40.0 37.0 40.0 33.0 40.0 36-37 36.191819250475284 38.5 37.0 40.0 33.0 40.0 38-39 36.131607251197224 37.0 37.0 40.0 33.0 40.0 40-41 36.012131458600706 37.0 37.0 40.0 30.0 40.0 42-43 35.868680709796884 37.0 37.0 40.0 27.0 40.0 44-45 35.72749944617826 37.0 37.0 40.0 27.0 40.0 46-47 35.61109940324063 37.0 37.0 40.0 27.0 40.0 48-49 35.465377722211436 37.0 37.0 40.0 27.0 40.0 50-51 34.33714075912253 37.0 35.0 38.5 24.5 38.5 52-53 34.75885036078658 37.0 35.0 38.5 27.0 40.0 54-55 35.48181188898729 37.0 37.0 40.0 27.0 40.0 56-57 35.50420023142119 37.0 37.0 40.0 27.0 40.0 58-59 35.32538987208691 37.0 37.0 40.0 27.0 40.0 60-61 35.0904104121644 37.0 33.0 40.0 27.0 40.0 62-63 34.87045122922116 37.0 33.0 40.0 27.0 40.0 64-65 34.64271275831641 37.0 33.0 40.0 27.0 40.0 66-67 34.40895473184767 37.0 33.0 40.0 27.0 40.0 68-69 34.16601299915651 37.0 33.0 38.5 27.0 40.0 70-71 33.84770283688279 37.0 33.0 37.0 24.5 40.0 72-73 33.610005835518365 37.0 33.0 37.0 22.0 40.0 74-75 33.37297210474887 37.0 33.0 37.0 22.0 40.0 76-77 33.10486437024022 37.0 33.0 37.0 22.0 37.0 78-79 32.80921525169816 37.0 33.0 37.0 22.0 37.0 80-81 32.51499291476512 37.0 33.0 37.0 22.0 37.0 82-83 32.21765089077162 37.0 33.0 37.0 22.0 37.0 84-85 32.02383064683748 37.0 33.0 37.0 22.0 37.0 86-87 31.81832226479151 33.0 33.0 37.0 22.0 37.0 88-89 31.546334752485095 33.0 33.0 37.0 15.0 37.0 90-91 31.430638365718092 33.0 33.0 37.0 15.0 37.0 92-93 31.24762198680194 33.0 33.0 37.0 15.0 37.0 94-95 31.070723299083205 33.0 33.0 37.0 15.0 37.0 96-97 30.90570941539051 33.0 27.0 37.0 15.0 37.0 98-99 30.735503288464564 33.0 27.0 37.0 15.0 37.0 100-101 29.354017325543523 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.29627203815416436 2306 2 0.2557008132756877 2306 3 0.24170504366649936 2306 4 0.25391458852666204 2306 5 0.35399572136444846 2306 6 0.3319696510520913 2306 7 0.3683651326694388 2306 8 0.2929921991097757 2306 9 0.3217602311091312 2306 10-11 0.43977668904552303 2306 12-13 0.34925593278495626 2306 14-15 0.5193646056033074 2306 16-17 0.4853836343597564 2306 18-19 0.5421262268697902 2306 20-21 0.47002914101222615 2306 22-23 0.4134280889448334 2306 24-25 0.5205196415500879 2306 26-27 0.4293543138901086 2306 28-29 0.24787362772281796 2306 30-31 0.2833992597764876 2306 32-33 0.2542361913208637 2306 34-35 0.23790020037858994 2306 36-37 0.19498836845266254 2306 38-39 0.12667654376503634 2306 40-41 0.2519743414711897 2306 42-43 0.15876224348829027 2306 44-45 0.13546040127133807 2306 46-47 0.178733004296177 2306 48-49 0.20487360128291954 2306 50-51 0.21695107308720907 2306 52-53 0.09083566936737242 2306 54-55 0.20867646443907262 2306 56-57 0.23384244689366795 2306 58-59 0.31282143520680705 2306 60-61 0.23885038147476934 2306 62-63 0.08642802136241556 2306 64-65 0.1824476852924306 2306 66-67 0.08546608979806081 2306 68-69 0.16646314890743952 2306 70-71 0.22883407001596368 2306 72-73 0.2066874513214927 2306 74-75 0.2890141752790427 2306 76-77 0.20595770785576661 2306 78-79 0.1410777121453961 2306 80-81 0.13596275705022265 2306 82-83 0.2513112564135582 2306 84-85 0.18995073810086893 2306 86-87 0.1724747576388559 2306 88-89 0.2895580832870621 2306 90-91 0.21123561775456778 2306 92-93 0.16072207153970197 2306 94-95 0.07065224706951057 2306 96-97 0.08861081138826066 2306 98-99 0.10195696295637546 2306 100-101 0.0952779054439894 2307 1 0.16867518609947751 2307 2 0.19367939426631864 2307 3 0.19964549232984297 2307 4 0.20732986579735524 2307 5 0.2229495163423394 2307 6 0.3044145822922033 2307 7 0.2955116987316657 2307 8 0.27232569470832857 2307 9 0.23298046558237928 2307 10-11 0.3278039496029024 2307 12-13 0.2083229077555515 2307 14-15 0.35748111203306365 2307 16-17 0.34090186891413055 2307 18-19 0.3266976438508422 2307 20-21 0.28836653097611986 2307 22-23 0.33165513855743 2307 24-25 0.34288222318977546 2307 26-27 0.3098187980895304 2307 28-29 0.25044046071133863 2307 30-31 0.3089980184475323 2307 32-33 0.21657098504119432 2307 34-35 0.2332048533338451 2307 36-37 0.23142968929156638 2307 38-39 0.1000713350513891 2307 40-41 0.24023526394898198 2307 42-43 0.23019889920205827 2307 44-45 0.25074882770434925 2307 46-47 0.2893374422500159 2307 48-49 0.20909635086710665 2307 50-51 0.1891881020109878 2307 52-53 0.16024989191411976 2307 54-55 0.20484193439972387 2307 56-57 0.11991197754150562 2307 58-59 0.1455160520580563 2307 60-61 0.21229628630621988 2307 62-63 0.23336161432010982 2307 64-65 0.09869469352739202 2307 66-67 0.21559853878593316 2307 68-69 0.2120631245676634 2307 70-71 0.15132626100212576 2307 72-73 -0.024213931989528703 2307 74-75 0.18581552208039653 2307 76-77 0.11940929197000827 2307 78-79 0.060602275043450504 2307 80-81 -0.04552866317613535 2307 82-83 0.09913568544280338 2307 84-85 0.2377114780302918 2307 86-87 0.23952518155675406 2307 88-89 0.4040323439661435 2307 90-91 0.22076829391374986 2307 92-93 0.26449210115310606 2307 94-95 0.10525855107340476 2307 96-97 0.2784978342270925 2307 98-99 0.3676311749558856 2307 100-101 0.08229202251834877 2201 1 0.2635118210404954 2201 2 0.1948435335822012 2201 3 0.19471589934976663 2201 4 0.3181642692418549 2201 5 0.2530011492060851 2201 6 0.25324359741224356 2201 7 0.26803148005130595 2201 8 0.26329073039968165 2201 9 0.3381810484782264 2201 10-11 0.330942876784988 2201 12-13 0.351537222695562 2201 14-15 0.5298594970464663 2201 16-17 0.42522957944276385 2201 18-19 0.45044439417502247 2201 20-21 0.428153342800222 2201 22-23 0.48554814003041 2201 24-25 0.44430476287831056 2201 26-27 0.4603440967764456 2201 28-29 0.38454348723750087 2201 30-31 0.2796061563282066 2201 32-33 0.41668590396454164 2201 34-35 0.3435148236225203 2201 36-37 0.310435367175522 2201 38-39 0.279461505450854 2201 40-41 0.27127989702675137 2201 42-43 0.2730725883158769 2201 44-45 0.2728167231104237 2201 46-47 0.22551062243155684 2201 48-49 0.2967406191628683 2201 50-51 0.23768223528389143 2201 52-53 0.26443800014641994 2201 54-55 0.2927122243369098 2201 56-57 0.3491840816318543 2201 58-59 0.27108612230770746 2201 60-61 0.2947747109766894 2201 62-63 0.36509820016190275 2201 64-65 0.2341390965312513 2201 66-67 0.3838225712795449 2201 68-69 0.32396075427142534 2201 70-71 0.3991278120338393 2201 72-73 0.36723981402902695 2201 74-75 0.4793598010772584 2201 76-77 0.3858435635390336 2201 78-79 0.20815545799469248 2201 80-81 0.3865446536019448 2201 82-83 0.3731710903854619 2201 84-85 0.3029416384840218 2201 86-87 0.47420528126593453 2201 88-89 0.5733296350112056 2201 90-91 0.5189439510879019 2201 92-93 0.41080029631620363 2201 94-95 0.28720636584345627 2201 96-97 0.18915279733973378 2201 98-99 0.24958866793721413 2201 100-101 0.2865518518041448 1101 1 0.23232550382576989 1101 2 0.22195341773831956 1101 3 0.18204914842587527 1101 4 0.31031291661949467 1101 5 0.3548263713120434 1101 6 0.3736327188249433 1101 7 0.3246441290200366 1101 8 0.28490479018704207 1101 9 0.4280739536281075 1101 10-11 0.2848810569661211 1101 12-13 0.39261641566811534 1101 14-15 0.5278101360297356 1101 16-17 0.5011250696238605 1101 18-19 0.3792386512614456 1101 20-21 0.4195844566415303 1101 22-23 0.3127135506765981 1101 24-25 0.3870570005934155 1101 26-27 0.3799867089855127 1101 28-29 0.1865128038918229 1101 30-31 0.297743503440536 1101 32-33 0.4208727409287789 1101 34-35 0.34034182884646924 1101 36-37 0.25681250250829635 1101 38-39 0.20682902321345153 1101 40-41 0.12459774564591442 1101 42-43 0.19118240487026839 1101 44-45 0.3622219650854177 1101 46-47 0.22903430116071632 1101 48-49 0.19775656778163153 1101 50-51 0.15655551046014438 1101 52-53 0.2507948819041488 1101 54-55 0.2691059955726587 1101 56-57 0.37805075339313987 1101 58-59 0.43171063978814317 1101 60-61 0.4826282729018274 1101 62-63 0.36164818526992804 1101 64-65 0.31473045732460747 1101 66-67 0.3908315353663667 1101 68-69 0.3579998765658843 1101 70-71 0.37147723653740883 1101 72-73 0.2952398120695605 1101 74-75 0.5366250148946605 1101 76-77 0.4401925956586794 1101 78-79 0.4416068126745003 1101 80-81 0.5493841685742566 1101 82-83 0.5566871198323824 1101 84-85 0.3816183518448568 1101 86-87 0.33594358616734965 1101 88-89 0.6533886322130797 1101 90-91 0.5253633595434088 1101 92-93 0.43460969765883206 1101 94-95 0.30792390672780456 1101 96-97 0.2501014479504988 1101 98-99 0.35732101947794703 1101 100-101 0.2620239371900226 2304 1 0.19329705017917576 2304 2 0.2122015347764119 2304 3 0.21551937748083105 2304 4 0.318830413442484 2304 5 0.3371582995270259 2304 6 0.3363996654821051 2304 7 0.28815108745539675 2304 8 0.3199643010818747 2304 9 0.299218143567046 2304 10-11 0.29321103247986713 2304 12-13 0.23676916029818784 2304 14-15 0.3666541678928681 2304 16-17 0.35404404802016387 2304 18-19 0.3182469579905174 2304 20-21 0.34329272927581655 2304 22-23 0.36402813704489034 2304 24-25 0.40557976661020945 2304 26-27 0.5299832220190197 2304 28-29 0.4067240365732232 2304 30-31 0.3838754502526811 2304 32-33 0.31858684067151444 2304 34-35 0.4738225188009082 2304 36-37 0.5317295901938834 2304 38-39 0.41951925660774236 2304 40-41 0.45804937754623154 2304 42-43 0.26156527379132655 2304 44-45 0.3741508774618225 2304 46-47 0.3683626339258055 2304 48-49 0.40502030642962694 2304 50-51 0.3230345266706678 2304 52-53 0.3674025284342264 2304 54-55 0.42508459334720783 2304 56-57 0.39809809864931367 2304 58-59 0.5452865626719401 2304 60-61 0.4937493713737595 2304 62-63 0.4887500511844465 2304 64-65 0.48677428461903105 2304 66-67 0.40209908643105763 2304 68-69 0.567552614996913 2304 70-71 0.4882231994050912 2304 72-73 0.434669654303697 2304 74-75 0.3795925783574532 2304 76-77 0.3508975827956462 2304 78-79 0.33689902046670284 2304 80-81 0.33609262718008637 2304 82-83 0.3901484377507387 2304 84-85 0.4898785880287235 2304 86-87 0.28302189568599445 2304 88-89 0.27540223913121764 2304 90-91 0.3850559640749154 2304 92-93 0.3655332788509078 2304 94-95 0.29186797578524093 2304 96-97 0.4621473674248193 2304 98-99 0.46128957478898514 2304 100-101 0.49708887225495957 2202 1 0.1947215045697348 2202 2 0.1485005621771336 2202 3 0.1140653700946217 2202 4 0.17483109825672472 2202 5 0.24975215576495202 2202 6 0.1943801971914496 2202 7 0.22484347541896454 2202 8 0.23627531273186975 2202 9 0.1595317314230016 2202 10-11 0.18455383907691214 2202 12-13 0.2175735724585266 2202 14-15 0.36125412223858433 2202 16-17 0.24171759543947502 2202 18-19 0.21818397953770585 2202 20-21 0.27803604622252465 2202 22-23 0.29823348756379886 2202 24-25 0.44147381606357783 2202 26-27 0.33374226115940786 2202 28-29 0.2835453163606019 2202 30-31 0.26912116812551545 2202 32-33 0.27253185987077444 2202 34-35 0.29249216522479315 2202 36-37 0.31114969927500624 2202 38-39 0.20477761093389546 2202 40-41 0.15194923161557483 2202 42-43 0.18754755422275338 2202 44-45 0.2998828745921216 2202 46-47 0.30659238910471487 2202 48-49 0.17291189381273142 2202 50-51 0.33022477051345334 2202 52-53 0.34952367941131257 2202 54-55 0.42488801497266593 2202 56-57 0.40082298675555705 2202 58-59 0.39519117783078883 2202 60-61 0.44700794581877545 2202 62-63 0.5014186051665561 2202 64-65 0.48810681900241093 2202 66-67 0.45127588264175245 2202 68-69 0.533635151418423 2202 70-71 0.46346369964558676 2202 72-73 0.45254432514446563 2202 74-75 0.5337724929187218 2202 76-77 0.45817679134604106 2202 78-79 0.5002114221265614 2202 80-81 0.43264511737664435 2202 82-83 0.5609220536514741 2202 84-85 0.5496808071529742 2202 86-87 0.5045400432634644 2202 88-89 0.7465040970974854 2202 90-91 0.6833448456265039 2202 92-93 0.5973228248353699 2202 94-95 0.5740671874336023 2202 96-97 0.6273207925558708 2202 98-99 0.5991666302507888 2202 100-101 0.5785202155507072 1102 1 0.10164240852453332 1102 2 0.06384896142383667 1102 3 0.0563964016911882 1102 4 0.12539607000814357 1102 5 0.27442782012987976 1102 6 0.13740174981752773 1102 7 0.1574392067435113 1102 8 0.07385639664063604 1102 9 0.1815133175288821 1102 10-11 0.16381989892206406 1102 12-13 0.15462630315533232 1102 14-15 0.2414016426403336 1102 16-17 0.20195770843383087 1102 18-19 0.25657067131422906 1102 20-21 0.213208153357904 1102 22-23 0.23969352104360553 1102 24-25 0.21956285142663035 1102 26-27 0.3039530793222056 1102 28-29 0.31916992401911415 1102 30-31 0.30836839557895956 1102 32-33 0.33790903082704205 1102 34-35 0.1874989658106827 1102 36-37 0.2996488622798168 1102 38-39 0.34664567956749437 1102 40-41 0.21040026739712658 1102 42-43 0.2917560706487876 1102 44-45 0.25891719139480074 1102 46-47 0.1604922635554331 1102 48-49 0.2833612556039 1102 50-51 0.17485230765511517 1102 52-53 0.38318134837971485 1102 54-55 0.436454242216854 1102 56-57 0.446496767881321 1102 58-59 0.42954983026853455 1102 60-61 0.3191281592525499 1102 62-63 0.4223539472883431 1102 64-65 0.45889830257637954 1102 66-67 0.46040436140300045 1102 68-69 0.4539322847099072 1102 70-71 0.43661184779374196 1102 72-73 0.42613189576594124 1102 74-75 0.5900944221926281 1102 76-77 0.33228486834959625 1102 78-79 0.5801209220219405 1102 80-81 0.5145738681613352 1102 82-83 0.5402927378950366 1102 84-85 0.5183457998292695 1102 86-87 0.39451179467589625 1102 88-89 0.595329688225334 1102 90-91 0.522377593063208 1102 92-93 0.5042097258606901 1102 94-95 0.37395471620531495 1102 96-97 0.3510182187956694 1102 98-99 0.4175125185119306 1102 100-101 0.21947543630819055 2305 1 0.1959461526690447 2305 2 0.15545917946717935 2305 3 0.1553089933850984 2305 4 0.22394525613694327 2305 5 0.09447831496806458 2305 6 0.11675784233710118 2305 7 0.1779924032521265 2305 8 0.23868862346861874 2305 9 0.12205915605773754 2305 10-11 0.09548996373968777 2305 12-13 0.15499785304917424 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-0.9283400806628599 1213 86-87 -0.930650620533811 1213 88-89 -0.8365253217000372 1213 90-91 -0.8968153997289505 1213 92-93 -0.9269189271546061 1213 94-95 -0.7865408359533212 1213 96-97 -0.8308581545682365 1213 98-99 -0.9743977709693006 1213 100-101 -0.8473852263034054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2930.0 3 329.0 4 290.0 5 284.0 6 557.0 7 2685.0 8 8213.0 9 12998.0 10 15639.0 11 14950.0 12 12036.0 13 10781.0 14 9988.0 15 9881.0 16 10465.0 17 11517.0 18 12906.0 19 14786.0 20 16728.0 21 18974.0 22 21388.0 23 24276.0 24 27376.0 25 31692.0 26 37101.0 27 43907.0 28 52601.0 29 63156.0 30 75882.0 31 95523.0 32 124687.0 33 170755.0 34 247125.0 35 386361.0 36 644004.0 37 1049927.0 38 515546.0 39 2618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2002954540958 24.426192533189123 16.142794019692545 16.230717993022534 2 37.57624098322778 21.518552631995416 28.38857606478888 12.516630319987932 3 43.1250908022795 19.226510916971844 22.200343249096065 15.448055031652588 4 36.05101926735939 19.664561474562614 28.639551243919627 15.644868014158368 5 37.29500555512629 17.541329849501306 28.84077053939709 16.322894055975315 6 33.71184739882564 17.91710895355691 31.652032190134356 16.7190114574831 7 40.30627365037313 18.151569098111345 22.22789678840061 19.31426046311491 8 34.48873890203471 26.297435356617914 20.49199496430855 18.72183077703883 9 33.82820519173923 22.718643456604497 23.093850184071137 20.359301167585134 10-11 29.165448214796143 28.35698041467382 27.793169403839997 14.684401966690041 12-13 29.42092395327044 27.338966229121336 26.771858229459323 16.468251588148906 14-15 27.861509845180148 27.85522219590285 27.386390284276885 16.896877674640116 16-17 27.30599945427151 27.919469457000158 27.646829304301633 17.1277017844267 18-19 26.514865430750902 28.28488249863611 27.777986474600564 17.422265596012423 20-21 25.761938034246334 28.685315258637473 27.78055235589362 17.772194351222574 22-23 26.062212343775055 28.659819950542463 27.264496608771193 18.01347109691128 24-25 24.783353032317347 29.328795561555864 27.673168873472775 18.214682532654006 26-27 24.68092542877986 29.107744812146763 27.65400945354318 18.557320305530194 28-29 24.506598690946774 29.280113065389607 27.448446366624076 18.764841877039544 30-31 24.542891743003082 28.570895246738992 28.002962873063446 18.88325013719448 32-33 23.999715991874623 28.591277466436367 28.359385887857968 19.04962065383105 34-35 23.362767439417524 29.40337077524769 28.163612139140554 19.07024964619423 36-37 23.678701977376342 29.043913354841592 27.8986241142819 19.378760553500165 38-39 23.130668762330426 28.937133914946898 28.511265226636983 19.420932096085693 40-41 23.20196646388146 28.9255622352766 28.259760776110138 19.612710524731806 42-43 23.490598077519316 28.444651433515222 28.221137568974104 19.84361291999136 44-45 22.91917112028756 28.795099470396746 28.40754304140188 19.878186367913813 46-47 23.30523412646122 28.695573411048475 27.83648870649022 20.162703756000084 48-49 22.680864314786284 28.828964638367733 28.111628405522147 20.378542641323833 50-51 22.450974916643858 28.34673936967401 28.590869582841012 20.61141613084112 52-53 23.3423942101921 28.308894489411447 27.898514658685702 20.45019664171075 54-55 22.996866756162778 28.44248651717886 28.321124298537597 20.239522428120768 56-57 23.228374007613752 28.192721514976103 28.199428968594333 20.379475508815812 58-59 22.82936346213483 28.651986440613523 27.918410799982414 20.600239297269233 60-61 22.25200417827206 28.42158052444837 28.09404939361606 21.23236590366351 62-63 22.73553892932562 28.437938502887235 27.97594159166281 20.850580976124334 64-65 22.154709028750823 28.722504325006685 28.376121133702647 20.746665512539845 66-67 22.14832703207374 28.374195847107032 28.097032799540766 21.380444321278464 68-69 21.95089730649684 28.427150727220514 28.660794600324337 20.96115736595831 70-71 21.820219939592345 28.75390609950533 28.45354347414203 20.972330486760296 72-73 21.819520507953598 29.279996622087456 27.81324366031131 21.087239209647635 74-75 21.48193831111083 29.45832878116389 28.16576335791241 20.893969549812873 76-77 21.67377820319004 29.209235932399036 28.03842855561322 21.078557308797706 78-79 21.534336839543954 29.59337387155694 27.898390179830418 20.97389910906869 80-81 21.445558802785673 29.434994277460795 28.057321999467867 21.06212492028566 82-83 21.632207058348946 29.519669584820164 27.704083632877364 21.14403972395353 84-85 21.40233008914211 29.507801172238242 27.99302543985333 21.09684329876632 86-87 21.6581918674135 29.022740058665796 27.94958521839427 21.36948285552643 88-89 21.62425577393505 29.425332810465036 27.686536145822704 21.263875269777206 90-91 21.528202517467605 29.087638692766365 27.959833579896788 21.42432520986924 92-93 21.63376902635941 29.09684766075078 27.736585086534998 21.532798226354817 94-95 21.3643558430509 29.147485746918473 27.973803464229807 21.514354945800818 96-97 21.52719305906973 28.842416596720312 27.929072414995055 21.701317929214902 98-99 21.393233879716377 29.12374660850111 27.81531191233098 21.66770759945153 100-101 21.463842730044743 28.89334660617335 27.94727398621029 21.69553667757162 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2051.0 1 1296.5 2 427.0 3 290.5 4 239.5 5 215.5 6 256.5 7 349.5 8 480.0 9 663.5 10 873.0 11 1110.5 12 1315.0 13 1349.5 14 1325.0 15 1245.5 16 1114.5 17 992.5 18 964.5 19 1086.0 20 1327.0 21 1804.5 22 2510.0 23 3524.0 24 5104.0 25 7248.0 26 10029.5 27 13706.5 28 18379.0 29 24330.0 30 31334.0 31 39575.0 32 49682.5 33 61383.0 34 74462.0 35 88850.5 36 105044.0 37 122274.5 38 137844.0 39 151493.0 40 163961.5 41 174603.5 42 182667.5 43 187477.5 44 189158.5 45 189758.0 46 189094.5 47 186095.0 48 181034.0 49 172735.5 50 161371.5 51 147333.5 52 130582.5 53 111802.0 54 92712.5 55 75544.5 56 61268.5 57 49657.5 58 40230.0 59 32253.5 60 25567.5 61 20241.0 62 15741.5 63 12013.5 64 9257.5 65 7130.5 66 5346.0 67 4079.0 68 3188.5 69 2473.0 70 1908.5 71 1521.5 72 1256.5 73 1009.5 74 768.5 75 616.0 76 503.5 77 417.5 78 338.5 79 265.5 80 214.5 81 185.5 82 161.5 83 130.0 84 116.0 85 100.5 86 85.5 87 68.0 88 53.5 89 51.5 90 49.0 91 39.0 92 27.0 93 21.5 94 17.0 95 9.5 96 7.5 97 7.0 98 4.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2653871674372813 2 0.2524164255371545 3 0.21787163017231354 4 0.21926605070113045 5 0.23334180509579144 6 0.2372356586479593 7 0.21337265073028172 8 0.20882105164565298 9 0.1899569097746774 10-11 0.17815695492233077 12-13 0.2064137029968465 14-15 0.20288818694285665 16-17 0.20434838202491962 18-19 0.1968106182229189 20-21 0.19712633607850008 22-23 0.16558086034168037 24-25 0.47607621639512304 26-27 0.33567385503604186 28-29 0.27596371559925087 30-31 0.2794629218319423 32-33 0.32197959305020807 34-35 0.3172964448590872 36-37 0.3098113006996834 38-39 0.3347267014692983 40-41 0.3406069465294978 42-43 0.37612520528238064 44-45 0.34272488714402155 46-47 0.3694162008512806 48-49 0.34461919427750864 50-51 0.3669825423811756 52-53 0.3556298544909023 54-55 0.3319115505903661 56-57 0.36912679281699784 58-59 0.3557745585080437 60-61 0.32136131224969494 62-63 0.298208669507075 64-65 0.304180998941819 66-67 0.3140603368393801 68-69 0.3207167216278834 70-71 0.2966695449611167 72-73 0.30360218287325347 74-75 0.30151055208002814 76-77 0.3051018426872641 78-79 0.3073250225869816 80-81 0.3245053358948575 82-83 0.3425933380375294 84-85 0.33701565592226185 86-87 0.341106833134168 88-89 0.3363052907472042 90-91 0.3098376105209818 92-93 0.29342028203076037 94-95 0.302720803859756 96-97 0.2908024548115664 98-99 0.31025856766175675 100-101 0.33347698495762274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3800862.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.24277254635837 #Duplication Level Percentage of deduplicated Percentage of total 1 97.04974721390631 91.4623725236175 2 2.2046955093175415 4.155532348371816 3 0.34464029741659996 0.9743957147922578 4 0.1360112215601525 0.5127229846898322 5 0.07704365454533527 0.363040380572812 6 0.05318959916308006 0.3007641177454876 7 0.01711468375376778 0.11290546737464342 8 0.016656814293125974 0.12558274886192017 9 0.023714652448106922 0.2011441137014593 >10 0.07263847920759071 1.2102689748543505 >50 0.0036205631283322283 0.22757169638562275 >100 8.272475220116135E-4 0.11998304752414393 >500 7.21462338123197E-5 0.04820244971868627 >1k 0.0 0.0 >5k 2.7917504226110325E-5 0.18551343178946206 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 7051 0.18551054997524247 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011576321371309982 0.0 0.0 2 0.0014470401714137477 0.0 0.0 3 0.001999546418680815 0.0 2.630982129843178E-5 4 0.002473123202052587 0.0 5.261964259686356E-5 5 0.0031308687345133813 0.0 5.261964259686356E-5 6 0.005709231221759695 0.0 7.892946389529534E-5 7 0.008024495496021692 0.0 7.892946389529534E-5 8 0.010602857983268005 0.0 7.892946389529534E-5 9 0.011892039226891164 0.0 1.3154910649215888E-4 10-11 0.01595690661749887 0.0 1.3154910649215888E-4 12-13 0.021008392306797775 0.0 1.4470401714137477E-4 14-15 0.024744386931175085 0.0 1.5785892779059067E-4 16-17 0.02855931101944769 0.0 1.5785892779059067E-4 18-19 0.03315037483602404 0.0 1.5785892779059067E-4 20-21 0.038964845342977464 0.0 1.5785892779059067E-4 22-23 0.045134498437459716 0.0 2.1047857038745423E-4 24-25 0.050462237250392145 0.0 2.36788391685886E-4 26-27 0.05572420151007851 0.0 2.36788391685886E-4 28-29 0.06074937737807898 0.0 2.4994330233510186E-4 30-31 0.08791426786870979 0.0 2.6309821298431776E-4 32-33 0.09632025577355874 0.0 2.8940803428274955E-4 34-35 0.10264776779583157 0.0 3.288727662303972E-4 36-37 0.10931730749498403 0.0 3.6833749817804487E-4 38-39 0.1174865070081471 0.0 3.6833749817804487E-4 40-41 0.12708959178207468 0.0 3.6833749817804487E-4 42-43 0.13795554797832704 0.0 3.9464731947647666E-4 44-45 0.151360401929878 0.0 4.0780223012569256E-4 46-47 0.16656747864037158 0.0 4.3411205142412435E-4 48-49 0.18228759686618456 0.0 4.472669620733402E-4 50-51 0.20125697802235387 0.0 4.604218727225561E-4 52-53 0.22191018774162283 0.0 4.73576783371772E-4 54-55 0.2442472260239914 0.0 4.8673169402098783E-4 56-57 0.2678208259073863 0.0 5.261964259686355E-4 58-59 0.2963275172842371 0.0 5.393513366178514E-4 60-61 0.32609708008341265 0.0 5.656611579162832E-4 62-63 0.3580108933184104 0.0 5.788160685654991E-4 64-65 0.392963490913377 0.0 5.788160685654991E-4 66-67 0.4325597719675168 0.0 6.051258898639309E-4 68-69 0.47487911952604434 0.0 6.314357111623627E-4 70-71 0.5204871947468759 0.0 6.314357111623627E-4 72-73 0.5693971525406605 0.0 6.577455324607944E-4 74-75 0.6229376388829692 0.0 6.577455324607944E-4 76-77 0.6782408832522728 0.0 6.577455324607944E-4 78-79 0.7381615012594511 0.0 6.577455324607944E-4 80-81 0.8016734098738654 0.0 6.577455324607944E-4 82-83 0.8708945497100394 0.0 6.840553537592262E-4 84-85 0.9439700783664338 0.0 6.840553537592262E-4 86-87 1.0246228355567764 0.0 7.10365175057658E-4 88-89 1.1086037851413706 0.0 7.629848176545215E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 875 0.0 65.616196 2 GATCGGG 1195 0.0 53.21495 1 GAGCGGC 870 0.0 45.798428 9 GATCCGG 360 0.0 38.22894 1 AGCGGCG 650 0.0 36.85288 10-11 TCGGGAG 1315 0.0 36.801716 3 TTGTGTT 32340 0.0 33.92693 1 CGCCGTA 1030 0.0 33.200935 58-59 AAGCGGC 375 0.0 32.887634 9 TCGCCGT 965 0.0 32.48845 56-57 GGTTCGG 410 0.0 32.409397 1 GTCGCCG 1120 0.0 32.233536 56-57 GGCGCCG 580 0.0 31.941032 56-57 CGGAGCG 120 1.519511E-7 31.637743 1 AGAGCGG 1335 0.0 31.267412 8 GTGTTTG 70810 0.0 30.956379 1 TCGGGAA 590 0.0 30.558022 3 GTTTGAT 17115 0.0 30.410488 3 TGTTTGA 72035 0.0 30.359657 2 GCGTCGG 270 0.0 29.866104 10-11 >>END_MODULE