FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3800862
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT70510.18551054997524247Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8750.065.6161962
GATCGGG11950.053.214951
GAGCGGC8700.045.7984289
GATCCGG3600.038.228941
AGCGGCG6500.036.8528810-11
TCGGGAG13150.036.8017163
TTGTGTT323400.033.926931
CGCCGTA10300.033.20093558-59
AAGCGGC3750.032.8876349
TCGCCGT9650.032.4884556-57
GGTTCGG4100.032.4093971
GTCGCCG11200.032.23353656-57
GGCGCCG5800.031.94103256-57
CGGAGCG1201.519511E-731.6377431
AGAGCGG13350.031.2674128
GTGTTTG708100.030.9563791
TCGGGAA5900.030.5580223
GTTTGAT171150.030.4104883
TGTTTGA720350.030.3596572
GCGTCGG2700.029.86610410-11
Produced by FastQC (version 0.11.2)