##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776572 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266167057796448 33.0 33.0 33.0 27.0 33.0 2 30.83234830575256 33.0 33.0 33.0 27.0 33.0 3 30.7030287707288 33.0 33.0 33.0 27.0 33.0 4 34.13262901160135 37.0 37.0 37.0 27.0 37.0 5 34.16820561469323 37.0 37.0 37.0 27.0 37.0 6 34.676742040184806 37.0 37.0 37.0 27.0 37.0 7 34.223195364643885 37.0 37.0 37.0 27.0 37.0 8 34.429061086836576 37.0 37.0 37.0 27.0 37.0 9 33.901432413782175 37.0 37.0 37.0 27.0 37.0 10-11 33.89849047674615 37.0 37.0 37.0 24.5 37.0 12-13 33.92531654140429 37.0 37.0 37.0 27.0 37.0 14-15 35.5496068533429 40.0 37.0 40.0 27.0 40.0 16-17 35.47125754347447 40.0 37.0 40.0 24.5 40.0 18-19 35.505917728767706 40.0 37.0 40.0 24.5 40.0 20-21 35.480688057071816 40.0 37.0 40.0 24.5 40.0 22-23 36.187466055265276 40.0 37.0 40.0 27.0 40.0 24-25 35.600368728057475 40.0 37.0 40.0 24.5 40.0 26-27 35.434008554433305 40.0 37.0 40.0 24.5 40.0 28-29 35.12655893670325 40.0 37.0 40.0 18.5 40.0 30-31 35.154538762185894 40.0 37.0 40.0 18.5 40.0 32-33 34.93766702250113 40.0 37.0 40.0 15.0 40.0 34-35 35.155066211140934 40.0 37.0 40.0 22.0 40.0 36-37 34.818921857599946 37.0 37.0 40.0 15.0 40.0 38-39 34.76038006577895 37.0 37.0 40.0 15.0 40.0 40-41 35.2170926955973 37.0 37.0 40.0 22.0 40.0 42-43 34.882903090573556 37.0 37.0 40.0 22.0 40.0 44-45 34.466310255955904 37.0 37.0 40.0 15.0 40.0 46-47 34.3406137136008 37.0 37.0 40.0 15.0 40.0 48-49 34.232663334500245 37.0 35.0 40.0 15.0 40.0 50-51 33.25746766156253 37.0 33.0 38.5 15.0 38.5 52-53 33.69394868924702 37.0 33.0 38.5 18.5 40.0 54-55 34.35816935415325 37.0 35.0 40.0 18.5 40.0 56-57 34.617394038404186 37.0 35.0 40.0 22.0 40.0 58-59 34.151680381419965 37.0 33.0 40.0 15.0 40.0 60-61 33.89989400599012 37.0 33.0 40.0 15.0 40.0 62-63 33.56098959436312 37.0 33.0 40.0 15.0 40.0 64-65 33.3296590183867 37.0 33.0 40.0 15.0 40.0 66-67 33.19993700865672 37.0 33.0 37.0 15.0 40.0 68-69 33.216850850617234 37.0 33.0 37.0 18.5 40.0 70-71 32.82176961375394 37.0 33.0 37.0 15.0 40.0 72-73 32.96717607178924 37.0 33.0 37.0 22.0 38.5 74-75 33.010163611820616 37.0 33.0 37.0 22.0 37.0 76-77 32.748567658249094 37.0 33.0 37.0 22.0 37.0 78-79 32.32046656812789 37.0 33.0 37.0 22.0 37.0 80-81 31.882813591723895 37.0 33.0 37.0 18.5 37.0 82-83 31.50292338898469 37.0 33.0 37.0 15.0 37.0 84-85 31.293892072670907 35.0 33.0 37.0 15.0 37.0 86-87 31.108786301957956 33.0 33.0 37.0 15.0 37.0 88-89 30.885572029106395 33.0 33.0 37.0 15.0 37.0 90-91 30.818661464568542 33.0 33.0 37.0 15.0 37.0 92-93 30.667754158725216 33.0 33.0 37.0 15.0 37.0 94-95 30.530906275796198 33.0 30.0 37.0 15.0 37.0 96-97 30.38697123647433 33.0 27.0 37.0 15.0 37.0 98-99 30.250381225482357 33.0 27.0 37.0 15.0 37.0 100-101 29.049926492091686 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.20201432544021358 2306 2 0.23205778101590724 2306 3 0.16132974426057345 2306 4 0.3340062957277965 2306 5 0.33693235200550475 2306 6 0.24691741404690504 2306 7 0.7120547734859741 2306 8 0.11340383861123371 2306 9 0.41189158433535766 2306 10-11 0.39241542943737784 2306 12-13 0.35381079086847933 2306 14-15 0.7536384831768359 2306 16-17 0.5696202724937862 2306 18-19 0.6067694139659707 2306 20-21 0.5724825812592726 2306 22-23 0.328359551846809 2306 24-25 0.6587968842757519 2306 26-27 0.3869942618906208 2306 28-29 0.45326395392254426 2306 30-31 0.5739126607925229 2306 32-33 0.5525817686207048 2306 34-35 0.31000896759235985 2306 36-37 0.6386937345497543 2306 38-39 0.5869363608739064 2306 40-41 0.16752810866218937 2306 42-43 0.4951783196545634 2306 44-45 0.6525070346271207 2306 46-47 0.6611864466834234 2306 48-49 0.5862049195330812 2306 50-51 0.43660984681987003 2306 52-53 0.44108266341010705 2306 54-55 0.5156022771705722 2306 56-57 0.45853873187642336 2306 58-59 0.7676855122213837 2306 60-61 0.6098064632194635 2306 62-63 0.5120547647718681 2306 64-65 0.5677523793669721 2306 66-67 0.45286103965099045 2306 68-69 0.33791619868429024 2306 70-71 0.5020276519759435 2306 72-73 0.09913375969887284 2306 74-75 0.28216242890803755 2306 76-77 0.27090157304436246 2306 78-79 0.37837369238233265 2306 80-81 0.3485209173614301 2306 82-83 0.41778128192501285 2306 84-85 0.6252740134878074 2306 86-87 0.5670370103739124 2306 88-89 0.5547555503163473 2306 90-91 0.505420055231216 2306 92-93 0.4954738182875751 2306 94-95 0.4108629511173092 2306 96-97 0.5190371593277447 2306 98-99 0.36498987205689915 2306 100-101 0.23390953173633378 2307 1 0.15427265117287092 2307 2 0.08456028483570321 2307 3 0.11500991905401037 2307 4 0.2576986151109182 2307 5 0.3777225003428484 2307 6 0.13488560262307203 2307 7 0.5023404474018633 2307 8 0.07191315503725804 2307 9 0.37720974705703014 2307 10-11 0.30366127594614767 2307 12-13 0.17402871273663578 2307 14-15 0.5037327289131142 2307 16-17 0.3878639317099086 2307 18-19 0.466803661036046 2307 20-21 0.27746552401782765 2307 22-23 0.23246577673564417 2307 24-25 0.48602401739069734 2307 26-27 0.265745860605044 2307 28-29 0.2653682331440166 2307 30-31 0.3708528257824213 2307 32-33 0.43728465163983543 2307 34-35 0.08779701373032367 2307 36-37 0.2989056484377457 2307 38-39 0.036806633225779706 2307 40-41 -0.08407733406124152 2307 42-43 0.23910439221062063 2307 44-45 0.2711762195266729 2307 46-47 0.16856126212503852 2307 48-49 0.22700656535553065 2307 50-51 0.19685136524780233 2307 52-53 0.17398836681575602 2307 54-55 0.2859544583978533 2307 56-57 0.14820478831226325 2307 58-59 0.30572081563526154 2307 60-61 0.3345977324131866 2307 62-63 0.37838700172138573 2307 64-65 0.305029303914381 2307 66-67 0.21888909786823518 2307 68-69 0.19195770322026107 2307 70-71 0.17539439068163887 2307 72-73 -0.1932421411765759 2307 74-75 -0.03521110221898027 2307 76-77 -0.04294721701847948 2307 78-79 0.09728778756686296 2307 80-81 0.1809353753796792 2307 82-83 0.2851176193803546 2307 84-85 0.271052328914287 2307 86-87 0.19909146452140902 2307 88-89 0.4408086457421092 2307 90-91 0.24387670882188672 2307 92-93 0.3770323073399311 2307 94-95 0.31022285188668874 2307 96-97 0.374697711614683 2307 98-99 0.31619284343449294 2307 100-101 0.23994957029347574 2201 1 0.1968862928967674 2201 2 0.03031048681889459 2201 3 -0.0046903571374841135 2201 4 0.19850121951481015 2201 5 0.14520994266773357 2201 6 0.03384381842376172 2201 7 0.5457495689287626 2201 8 7.880907441517593E-4 2201 9 0.2639572724091792 2201 10-11 0.13772967966195893 2201 12-13 0.15712269143001123 2201 14-15 0.45513289412235025 2201 16-17 0.3366030320705491 2201 18-19 0.33446750138460857 2201 20-21 0.28625325506263977 2201 22-23 0.08896544673228135 2201 24-25 0.49997444254597667 2201 26-27 0.33779159465591846 2201 28-29 0.4479383951796265 2201 30-31 0.3640612471512412 2201 32-33 0.3612283106884391 2201 34-35 0.14753592427771167 2201 36-37 0.3929990599555353 2201 38-39 0.3675106630404201 2201 40-41 0.20737639018425824 2201 42-43 0.5216373651880772 2201 44-45 0.5702592667879074 2201 46-47 0.49662800091822845 2201 48-49 0.459477330152005 2201 50-51 0.3387446977459021 2201 52-53 0.32547849520573635 2201 54-55 0.47570705210352315 2201 56-57 0.6115825283873519 2201 58-59 0.5976464314811594 2201 60-61 0.521058783148419 2201 62-63 0.5585972340520513 2201 64-65 0.4824817722980228 2201 66-67 0.447642466298781 2201 68-69 0.3776807275680554 2201 70-71 0.5079677791652202 2201 72-73 0.25429336201534625 2201 74-75 0.36585760678296 2201 76-77 0.4144449903738163 2201 78-79 0.31897834818766313 2201 80-81 0.6637312378280917 2201 82-83 0.5155716655159246 2201 84-85 0.7561194409188268 2201 86-87 0.7448690064057963 2201 88-89 0.8185551359893672 2201 90-91 0.6235582187844422 2201 92-93 0.6152203001987253 2201 94-95 0.6053962366721208 2201 96-97 0.6414214669133251 2201 98-99 0.5418811806364197 2201 100-101 0.4029798162620182 1101 1 0.13568012040868993 1101 2 0.13257895701601186 1101 3 0.034760641446258234 1101 4 0.08280185946588858 1101 5 0.21633834405517405 1101 6 0.20462641428463968 1101 7 0.5263489651748046 1101 8 -0.011438454047151936 1101 9 0.22946984827378003 1101 10-11 0.21673534820173757 1101 12-13 0.22537693580627405 1101 14-15 0.363525530994913 1101 16-17 0.296993715001328 1101 18-19 0.31329517144958885 1101 20-21 0.2045765551157004 1101 22-23 0.14806463036415352 1101 24-25 0.3457167120001756 1101 26-27 0.15399191586146088 1101 28-29 0.19488975397746344 1101 30-31 0.20108264562534828 1101 32-33 0.2948420838695327 1101 34-35 0.07868477879690516 1101 36-37 0.3410906281343884 1101 38-39 0.0925209423205473 1101 40-41 -0.018905005548703002 1101 42-43 0.3895737425940453 1101 44-45 0.4487694213109634 1101 46-47 0.26728759684587544 1101 48-49 0.21001729985887607 1101 50-51 0.1639971111803149 1101 52-53 0.11199383681169905 1101 54-55 0.2198680695589701 1101 56-57 0.381936733052207 1101 58-59 0.45780288739622677 1101 60-61 0.42149947433775736 1101 62-63 0.41937905677049514 1101 64-65 0.3243664124177812 1101 66-67 0.35440060977293086 1101 68-69 0.21399434928351724 1101 70-71 0.3459137857088166 1101 72-73 0.2102943530433805 1101 74-75 0.35334745928847155 1101 76-77 0.536776155394044 1101 78-79 0.3983779679421602 1101 80-81 0.6059560606203327 1101 82-83 0.7080498681706082 1101 84-85 0.5316948170350493 1101 86-87 0.4157932279040786 1101 88-89 0.4165623613568741 1101 90-91 0.5382677807992486 1101 92-93 0.4643280235402507 1101 94-95 0.5073106427757281 1101 96-97 0.316628194658243 1101 98-99 0.3213101446606963 1101 100-101 0.31463265422190645 2304 1 0.17700591742391225 2304 2 0.13434404204749484 2304 3 0.08574482509443371 2304 4 0.23689973243926232 2304 5 0.29539531267037233 2304 6 0.20522138676716395 2304 7 0.4962185670390511 2304 8 0.1984827120989081 2304 9 0.21982137784886646 2304 10-11 0.19317691694585193 2304 12-13 0.23281161278579532 2304 14-15 0.3775148214902302 2304 16-17 0.3009007009698834 2304 18-19 0.335433105903455 2304 20-21 0.27800111756302215 2304 22-23 0.3162583930692975 2304 24-25 0.4442367179498987 2304 26-27 0.26431716748523826 2304 28-29 0.282876267934526 2304 30-31 0.31285814267526746 2304 32-33 0.32581783874086767 2304 34-35 0.2048486937158387 2304 36-37 0.4043337239745384 2304 38-39 0.27235137516303354 2304 40-41 0.09712702380327443 2304 42-43 0.3166202000287228 2304 44-45 0.3227939143448282 2304 46-47 0.2305455669980887 2304 48-49 0.42099702121343086 2304 50-51 0.28436252344666 2304 52-53 0.296281605169078 2304 54-55 0.43507209553943227 2304 56-57 0.36869363721205417 2304 58-59 0.4852088073724232 2304 60-61 0.4219828894798141 2304 62-63 0.26178076235827774 2304 64-65 0.3625264600155873 2304 66-67 0.40257354844118254 2304 68-69 0.2972582691605581 2304 70-71 0.3172088424791113 2304 72-73 0.04685541209439492 2304 74-75 0.37696042968859444 2304 76-77 0.3731488064922672 2304 78-79 0.4381791479709207 2304 80-81 0.39092061017658253 2304 82-83 0.49244671481645597 2304 84-85 0.47683835414645515 2304 86-87 0.4078627405224502 2304 88-89 0.42703139799726486 2304 90-91 0.4271368372720552 2304 92-93 0.38814427620522807 2304 94-95 0.3413331525679837 2304 96-97 0.38103148683931565 2304 98-99 0.3826617238168275 2304 100-101 0.3895297735634706 2202 1 0.14842026912312534 2202 2 -0.02570506760456226 2202 3 0.035944372305952044 2202 4 0.14007364780361797 2202 5 0.16179727705645774 2202 6 0.11626069646882797 2202 7 0.37214541752151575 2202 8 0.10473351672476383 2202 9 0.20166713460537267 2202 10-11 0.21970288006337313 2202 12-13 0.14355246517110487 2202 14-15 0.17835916838523502 2202 16-17 0.21602703900381215 2202 18-19 0.18734913593444702 2202 20-21 0.24611862276757535 2202 22-23 0.11142608494704831 2202 24-25 0.34145750100393 2202 26-27 0.2687356231688014 2202 28-29 0.22411220464461934 2202 30-31 0.23308086311135412 2202 32-33 0.23442113386035857 2202 34-35 0.1616334559076904 2202 36-37 0.40269370023612083 2202 38-39 0.2130549243087927 2202 40-41 0.11427018597502325 2202 42-43 0.26992458253136675 2202 44-45 0.4269495517498001 2202 46-47 0.2374047472688261 2202 48-49 0.21203356771217585 2202 50-51 0.4148627887769365 2202 52-53 0.24410354186033345 2202 54-55 0.3614098154574208 2202 56-57 0.3923039502389898 2202 58-59 0.4382486445946583 2202 60-61 0.3017970640828409 2202 62-63 0.41677462365176865 2202 64-65 0.3573503215288767 2202 66-67 0.3511147028948116 2202 68-69 0.386797819072477 2202 70-71 0.48696904042491695 2202 72-73 0.27498513191190455 2202 74-75 0.49117600975710474 2202 76-77 0.4183417753968115 2202 78-79 0.45018557869773446 2202 80-81 0.4270005044212759 2202 82-83 0.5174905963629648 2202 84-85 0.43207368169590765 2202 86-87 0.32287164243188826 2202 88-89 0.5383025969447353 2202 90-91 0.4754344844675593 2202 92-93 0.36580519352580154 2202 94-95 0.38303161176834877 2202 96-97 0.4229913513167709 2202 98-99 0.4414950241801243 2202 100-101 0.5728761379106153 1102 1 -0.09582708123739891 1102 2 -0.22102186602925755 1102 3 -0.14268277419160214 1102 4 -0.15276912818843158 1102 5 -0.12285918680902341 1102 6 -0.07384580793758033 1102 7 0.11868435612130668 1102 8 -0.022086602195301452 1102 9 -0.10836451386614243 1102 10-11 -0.004535227929949315 1102 12-13 -0.10640032756821682 1102 14-15 -0.006999160363108103 1102 16-17 -0.13087408335258743 1102 18-19 -0.07677684501131665 1102 20-21 -0.09876706628347875 1102 22-23 -0.09430934082926257 1102 24-25 -0.009221559604760898 1102 26-27 0.0423664014581675 1102 28-29 -0.02681806495257888 1102 30-31 0.110560526283777 1102 32-33 0.03735751596829573 1102 34-35 -0.06803590021543471 1102 36-37 0.028834969698174007 1102 38-39 0.01848544849339362 1102 40-41 -0.1008751701577566 1102 42-43 0.023344318725733615 1102 44-45 0.03224421554964607 1102 46-47 -0.04199738257798913 1102 48-49 0.021771415085218848 1102 50-51 -0.08620865013656243 1102 52-53 -0.060086924505178274 1102 54-55 0.0899169378717275 1102 56-57 0.16830504580941152 1102 58-59 0.11158375159375566 1102 60-61 0.07508743730072354 1102 62-63 0.11689706088571938 1102 64-65 0.17016116550420435 1102 66-67 0.10735842870297319 1102 68-69 0.16196245627940442 1102 70-71 0.10361697500922418 1102 72-73 0.09829589625325497 1102 74-75 0.3657702987946436 1102 76-77 0.38169642288375627 1102 78-79 0.31629206259236753 1102 80-81 0.3092996820195033 1102 82-83 0.23081221384962447 1102 84-85 0.17687228617085182 1102 86-87 0.09975670527033387 1102 88-89 0.3516832461305377 1102 90-91 0.3182703528157127 1102 92-93 0.3034741140752253 1102 94-95 0.3055488115000067 1102 96-97 0.41729691893807797 1102 98-99 0.23595777840555243 1102 100-101 0.2774541562795214 2305 1 0.15798345345125142 2305 2 0.07208757848405511 2305 3 0.015740001038071938 2305 4 0.03828257881173869 2305 5 0.046297620301793074 2305 6 0.28766660087013207 2305 7 0.2138511651145052 2305 8 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-0.9891625414866212 1213 80-81 -1.3673494328216727 1213 82-83 -1.5413089246673977 1213 84-85 -1.4213621462468886 1213 86-87 -1.3120455242265017 1213 88-89 -1.3440886212307142 1213 90-91 -1.327396931867252 1213 92-93 -1.416000803983703 1213 94-95 -1.3161141954849214 1213 96-97 -1.4722069348146114 1213 98-99 -1.3470425930551961 1213 100-101 -1.2981844368240836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2063.0 3 301.0 4 317.0 5 242.0 6 448.0 7 2923.0 8 15292.0 9 43475.0 10 60700.0 11 43447.0 12 24108.0 13 15463.0 14 11341.0 15 9254.0 16 8430.0 17 8046.0 18 8039.0 19 8554.0 20 9113.0 21 10179.0 22 11273.0 23 12063.0 24 13521.0 25 15783.0 26 18715.0 27 22909.0 28 27961.0 29 33916.0 30 40629.0 31 52663.0 32 71276.0 33 101404.0 34 153208.0 35 259380.0 36 502115.0 37 874350.0 38 282768.0 39 903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1225309572511 23.90659837626758 20.015526016006238 14.955344650475077 2 32.73095059043882 25.903384576060628 27.89310329764117 13.472561535859379 3 37.038943153044166 20.460602105956095 26.025657497740728 16.474797243259008 4 30.77348676460398 18.65861686646802 30.223166851454046 20.344729517473954 5 35.28880087815017 16.80873558720878 28.741297639411933 19.161165895229118 6 33.28757386880691 17.129766164684256 31.945183939691358 17.637476026817478 7 36.60741803630705 18.764404050348585 25.098649474795753 19.529528438548613 8 29.79405685052918 30.009245572806904 21.563007026924033 18.63369054973988 9 32.39388952611319 26.092476860579694 22.282721447670976 19.230912165636145 10-11 27.313928513641418 29.939591355515578 26.450234074703676 16.296246056139328 12-13 28.923841902020015 25.397881071062677 26.375645633150818 19.302631393766482 14-15 26.421375234690153 25.52223633961664 27.94684770931944 20.10954071637377 16-17 27.665037632105705 25.998233237862262 28.421649187293024 17.91507994273901 18-19 27.232507635654308 26.133576004822157 28.758537959607395 17.87537839991614 20-21 25.507166261118886 28.173515874638195 28.094114267950925 18.225203596291994 22-23 28.199296641253675 26.15698076972675 27.24347326261029 18.40024932640928 24-25 25.002890010802037 29.05123885129714 27.460602233024055 18.485268904876765 26-27 23.17975691914033 30.869531285848538 27.536644770191792 18.414067024819335 28-29 24.35651123166607 29.822852346981595 27.17434091019073 18.646295511161608 30-31 26.33581652412516 27.677808522732605 27.43098816981327 18.555386783328963 32-33 22.982487357098407 29.838277440222267 28.893340787956028 18.285894414723302 34-35 26.868068236811077 27.814374659378643 27.481182165883077 17.836374937927204 36-37 23.803266477155937 29.21653244495212 29.35913511435849 17.621065963533454 38-39 23.063632742031793 31.326684089239947 27.565178822268976 18.04450434645928 40-41 27.240040855065516 27.03198934284811 27.040806462949035 18.687163339137335 42-43 27.10555577244801 26.927902780127443 27.37350832216337 18.593033125261176 44-45 24.163362094121187 28.55477718595509 28.62041993653269 18.66144078339103 46-47 24.487779840124922 29.107349084685357 27.711505547553166 18.69336552763656 48-49 21.483463854980847 29.44240234275832 29.10713146719992 19.96700233506091 50-51 20.2687724205187 28.047351629458557 30.346346579357856 21.337529370664885 52-53 25.09376838684402 27.413167112649283 27.23860503577258 20.254459464734122 54-55 26.44712473833049 27.135419197847348 27.439729972063898 18.977726091758264 56-57 26.914114721545936 27.042000395079256 26.849738128961746 19.194146754413058 58-59 25.455973038540254 27.453524460928925 26.88382809358807 20.206674406942756 60-61 22.056476983549057 27.474736125033445 26.843159573609054 23.625627317808444 62-63 24.340325575800748 27.522359992905777 26.8363260439329 21.300988387360576 64-65 22.27286560998892 28.708714024204667 28.84836815806276 20.17005220774365 66-67 21.04529683440221 28.62439342660859 27.9132141236586 22.41709561533059 68-69 20.857607896481518 27.457127734331955 32.11049148877363 19.574772880412887 70-71 20.245026151534415 30.904015922094434 29.73593813211582 19.115019794255332 72-73 19.972043755370038 34.13824487596787 26.64343180874446 19.24627955991763 74-75 20.29503580121242 34.03231970616347 26.374324415119233 19.298320077504876 76-77 20.56459893784134 33.440784645642765 26.279224219225966 19.71539219728993 78-79 20.27677254635981 33.27534650755963 26.78095447964937 19.666926466431192 80-81 19.66689928881934 33.27711982582063 26.987632245513144 20.068348639846892 82-83 19.690087281187225 32.69671151603588 26.946083548979622 20.66711765379727 84-85 19.733696224638003 32.24148556580202 27.472358114745465 20.552460094814506 86-87 20.028431829917817 31.82944804740758 28.04721606203256 20.094904060642044 88-89 20.548283285840547 32.04093461172391 27.62213643087939 19.788645671556147 90-91 20.947468088749655 31.43298799991041 27.048428803972048 20.57111510736789 92-93 20.730175523066467 31.719343693376345 26.842428726770134 20.708052056787054 94-95 20.40253427572151 31.737201286283284 27.188285458944634 20.671978979050575 96-97 20.651350641667214 32.07714815214796 26.851382642857686 20.42011856332714 98-99 21.007896692377248 31.541630586235215 26.732790943748487 20.717681777639054 100-101 21.200886936227203 31.499713911937633 26.372043839495113 20.92735531234005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1443.0 1 925.5 2 321.5 3 188.5 4 143.5 5 145.5 6 176.5 7 250.5 8 361.5 9 516.0 10 651.5 11 810.5 12 906.0 13 849.5 14 774.5 15 715.0 16 644.0 17 552.0 18 520.5 19 585.0 20 665.0 21 784.5 22 1003.0 23 1347.0 24 1876.5 25 2592.5 26 3576.5 27 4880.5 28 6444.0 29 8400.5 30 10783.5 31 14175.0 32 19832.0 33 29185.0 34 42520.0 35 60926.5 36 87093.5 37 119485.5 38 150948.0 39 170961.5 40 177862.5 41 170937.5 42 160367.5 43 157452.5 44 150466.0 45 143243.0 46 144291.0 47 138039.0 48 119011.0 49 99950.0 50 84960.0 51 75075.0 52 67832.0 53 59415.0 54 51019.5 55 43169.0 56 36085.5 57 29236.5 58 23395.5 59 18952.5 60 15572.5 61 12701.0 62 10311.0 63 8304.5 64 6520.0 65 5250.5 66 4186.0 67 3291.5 68 2567.0 69 2006.0 70 1558.5 71 1211.5 72 958.0 73 716.5 74 543.5 75 420.0 76 297.5 77 217.0 78 187.5 79 157.5 80 117.5 81 86.5 82 67.0 83 59.0 84 53.5 85 38.5 86 25.0 87 28.0 88 26.5 89 20.0 90 17.0 91 12.5 92 7.0 93 7.5 94 11.0 95 9.0 96 4.5 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.253081857772822 2 0.24069247979162794 3 0.2075941124523333 4 0.209610987937644 5 0.22340497563182227 6 0.22650232012712077 7 0.20348833021437948 8 0.19866223530310037 9 0.18018621523230804 10-11 0.16929148604826383 12-13 0.1964652816494584 14-15 0.19322387461949483 16-17 0.19452043743148026 18-19 0.18722727161406225 20-21 0.18791156865372122 22-23 0.15495726384909161 24-25 0.45889319635867537 26-27 0.31877437358008365 28-29 0.27105365897228667 30-31 0.272062096714942 32-33 0.31385823958463893 34-35 0.31162527029733067 36-37 0.30220718209360314 38-39 0.3273641022094871 40-41 0.33237027528909746 42-43 0.36822383860386115 44-45 0.33568371358639354 46-47 0.3614889151082702 48-49 0.33878105808169207 50-51 0.3599222350437878 52-53 0.34747883361209436 54-55 0.32437480461518736 56-57 0.36154293855876957 58-59 0.3478930133992563 60-61 0.3146505835252967 62-63 0.29255499227104503 64-65 0.29909182978147153 66-67 0.3084378867178665 68-69 0.31538890401545505 70-71 0.2914385076273909 72-73 0.2995600330191329 74-75 0.296408665073335 76-77 0.30042440822712324 78-79 0.3030535494847603 80-81 0.3187563657632505 82-83 0.3352695337992316 84-85 0.33020933726912177 86-87 0.33318062704658835 88-89 0.3289667979076357 90-91 0.30346772927192234 92-93 0.28879135855292065 94-95 0.29842554055864573 96-97 0.2854238968051252 98-99 0.3043681201135789 100-101 0.32657175826882934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2776572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.606121464650826 #Duplication Level Percentage of deduplicated Percentage of total 1 91.34576728368293 54.44766899992932 2 4.344067670186013 5.178660503995404 3 1.271827893991439 2.2742618379415425 4 0.5946583114212889 1.4178110216216602 5 0.4413161980024631 1.3152573451226355 6 0.3324786597897225 1.1890658027898313 7 0.18468593588234697 0.7705888628911122 8 0.1618507077070187 0.7717834354179403 9 0.1266247245439174 0.6792847840433425 >10 0.9883655990227077 11.337278327906478 >50 0.10140174763802695 4.2762102972297935 >100 0.0982193858943948 11.609904239427468 >500 0.006526164515258847 2.524370435932717 >1k 0.002149448765539383 1.873186235874048 >5k 6.0268957058483645E-5 0.33466786987674557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 9316 0.3355216432348954 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004213829138952636 0.0 0.0 2 0.0047180480102803025 0.0 0.0 3 0.005330313782606754 0.0 0.0 4 0.005690470119269373 0.0 0.0 5 0.007131095465919847 0.0 0.0 6 0.008499689545237797 3.601563366626185E-5 0.0 7 0.010804690099878556 3.601563366626185E-5 3.601563366626185E-5 8 0.012389377981194077 3.601563366626185E-5 3.601563366626185E-5 9 0.01458633163483605 3.601563366626185E-5 3.601563366626185E-5 10-11 0.023590240051401513 3.601563366626185E-5 3.601563366626185E-5 12-13 0.03223399213130436 3.601563366626185E-5 3.601563366626185E-5 14-15 0.041039814562705376 3.601563366626185E-5 3.601563366626185E-5 16-17 0.048134894394958966 3.601563366626185E-5 3.601563366626185E-5 18-19 0.055554114930208905 3.601563366626185E-5 5.4023450499392776E-5 20-21 0.06245110877729805 3.601563366626185E-5 7.20312673325237E-5 22-23 0.06875384466889387 3.601563366626185E-5 7.20312673325237E-5 24-25 0.07565083851598302 3.601563366626185E-5 7.20312673325237E-5 26-27 0.08316009813539862 3.601563366626185E-5 7.20312673325237E-5 28-29 0.09212799091829782 3.601563366626185E-5 7.20312673325237E-5 30-31 0.1934939918719918 3.601563366626185E-5 7.20312673325237E-5 32-33 0.20653165125917858 3.601563366626185E-5 7.20312673325237E-5 34-35 0.2162558723490693 3.601563366626185E-5 7.20312673325237E-5 36-37 0.22743872660244357 3.601563366626185E-5 7.20312673325237E-5 38-39 0.24011622965296775 3.601563366626185E-5 7.20312673325237E-5 40-41 0.2547565847383032 3.601563366626185E-5 7.20312673325237E-5 42-43 0.2737368236804232 3.601563366626185E-5 7.20312673325237E-5 44-45 0.29374350818203165 3.601563366626185E-5 7.20312673325237E-5 46-47 0.31681152154527237 3.601563366626185E-5 7.20312673325237E-5 48-49 0.34222055109682015 3.601563366626185E-5 7.20312673325237E-5 50-51 0.37038477662383684 3.601563366626185E-5 7.20312673325237E-5 52-53 0.4022586124184786 3.601563366626185E-5 7.20312673325237E-5 54-55 0.4363113940499292 3.601563366626185E-5 7.20312673325237E-5 56-57 0.4742718719341692 3.601563366626185E-5 7.20312673325237E-5 58-59 0.5176887183188479 3.601563366626185E-5 7.20312673325237E-5 60-61 0.5632845105403353 3.601563366626185E-5 7.20312673325237E-5 62-63 0.6116715143709581 3.601563366626185E-5 7.20312673325237E-5 64-65 0.662399534389888 3.601563366626185E-5 7.20312673325237E-5 66-67 0.7195743528350786 3.601563366626185E-5 7.20312673325237E-5 68-69 0.7790181562012438 3.601563366626185E-5 7.20312673325237E-5 70-71 0.8407489523052167 3.601563366626185E-5 7.20312673325237E-5 72-73 0.9064234602956451 3.601563366626185E-5 7.20312673325237E-5 74-75 0.9767980084795208 3.601563366626185E-5 7.20312673325237E-5 76-77 1.0539615036094867 3.601563366626185E-5 7.20312673325237E-5 78-79 1.137067578294386 3.601563366626185E-5 7.20312673325237E-5 80-81 1.2294116630146814 3.601563366626185E-5 7.20312673325237E-5 82-83 1.326887975532419 3.601563366626185E-5 7.20312673325237E-5 84-85 1.433224133932057 3.601563366626185E-5 7.20312673325237E-5 86-87 1.54953662285725 3.601563366626185E-5 1.0804690099878555E-4 88-89 1.6734304026691906 3.601563366626185E-5 1.0804690099878555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2150 0.0 86.4872 9 CGGGAGA 3370 0.0 82.653534 4 AAGCGGC 840 0.0 81.88285 9 GAGAGCG 2960 0.0 80.93917 7 AGAGCGG 2345 0.0 80.10877 8 ATTCGGA 600 0.0 79.89316 2 ATCGGGA 4200 0.0 78.650116 2 TCGGGAG 3740 0.0 77.52301 3 CGGAAAG 1475 0.0 76.24329 5 GGATCGG 1605 0.0 73.339775 1 GATCGGG 4850 0.0 71.04898 1 GTTCGGA 575 0.0 70.16016 2 AAAGCGG 1015 0.0 70.10564 8 TCGGAGA 900 0.0 68.01432 4 TTCGGAG 580 0.0 67.08831 3 CCGGAAA 665 0.0 66.36124 4 ACCGGAA 405 0.0 65.613625 3 TCGGAAA 1405 0.0 65.520706 4 GATCCGG 665 0.0 64.95053 1 TCGGGAA 985 0.0 63.591446 3 >>END_MODULE