FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776572
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT93160.3355216432348954Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC21500.086.48729
CGGGAGA33700.082.6535344
AAGCGGC8400.081.882859
GAGAGCG29600.080.939177
AGAGCGG23450.080.108778
ATTCGGA6000.079.893162
ATCGGGA42000.078.6501162
TCGGGAG37400.077.523013
CGGAAAG14750.076.243295
GGATCGG16050.073.3397751
GATCGGG48500.071.048981
GTTCGGA5750.070.160162
AAAGCGG10150.070.105648
TCGGAGA9000.068.014324
TTCGGAG5800.067.088313
CCGGAAA6650.066.361244
ACCGGAA4050.065.6136253
TCGGAAA14050.065.5207064
GATCCGG6650.064.950531
TCGGGAA9850.063.5914463
Produced by FastQC (version 0.11.2)