##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339526 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.112284710663612 33.0 33.0 33.0 27.0 33.0 2 30.734302162061887 33.0 33.0 33.0 27.0 33.0 3 30.579852072599323 33.0 33.0 33.0 27.0 33.0 4 34.04184266385584 37.0 37.0 37.0 27.0 37.0 5 34.093078683459815 37.0 37.0 37.0 27.0 37.0 6 34.52823734380383 37.0 37.0 37.0 27.0 37.0 7 34.058039534504 37.0 37.0 37.0 27.0 37.0 8 34.29443912997761 37.0 37.0 37.0 27.0 37.0 9 33.76580683437585 37.0 37.0 37.0 22.0 37.0 10-11 33.76209903202614 37.0 37.0 37.0 22.0 37.0 12-13 33.77279521578303 37.0 37.0 37.0 24.5 37.0 14-15 35.31176913614125 40.0 37.0 40.0 22.0 40.0 16-17 35.2246756394244 40.0 37.0 40.0 22.0 40.0 18-19 35.250992508738946 40.0 37.0 40.0 22.0 40.0 20-21 35.26956657032237 40.0 37.0 40.0 22.0 40.0 22-23 35.85567097779636 40.0 37.0 40.0 27.0 40.0 24-25 35.31183432028539 38.5 37.0 40.0 22.0 40.0 26-27 35.15057836501924 37.0 37.0 40.0 24.5 40.0 28-29 34.87300825038918 37.0 37.0 40.0 18.5 40.0 30-31 34.919589480946144 37.0 37.0 40.0 18.5 40.0 32-33 34.700184139864234 37.0 37.0 40.0 15.0 40.0 34-35 34.75925956796377 37.0 37.0 40.0 18.5 40.0 36-37 34.590957527293995 37.0 37.0 40.0 15.0 40.0 38-39 34.71978041705884 37.0 37.0 40.0 18.5 40.0 40-41 34.42060122435058 37.0 37.0 40.0 15.0 40.0 42-43 34.242847696499204 37.0 33.0 40.0 15.0 40.0 44-45 34.13402009637849 37.0 33.0 40.0 15.0 40.0 46-47 34.081382724534805 37.0 33.0 40.0 15.0 40.0 48-49 33.97344547570747 37.0 33.0 40.0 15.0 40.0 50-51 32.96019834787047 37.0 33.0 38.5 15.0 38.5 52-53 33.42585891330124 37.0 33.0 38.5 18.5 40.0 54-55 34.08441368037799 37.0 33.0 40.0 18.5 40.0 56-57 34.28475917771378 37.0 33.0 40.0 18.5 40.0 58-59 33.817529918453566 37.0 33.0 40.0 15.0 40.0 60-61 33.605664779959696 37.0 33.0 40.0 15.0 40.0 62-63 33.26797842810895 37.0 33.0 40.0 15.0 40.0 64-65 33.04076124821866 37.0 33.0 37.0 15.0 40.0 66-67 32.88804655301972 37.0 33.0 37.0 15.0 40.0 68-69 32.89013522397272 37.0 33.0 37.0 18.5 40.0 70-71 32.49203236040121 37.0 33.0 37.0 15.0 38.5 72-73 32.62914560470796 37.0 33.0 37.0 22.0 37.0 74-75 32.62879510635915 37.0 33.0 37.0 22.0 37.0 76-77 32.319388842013296 37.0 33.0 37.0 22.0 37.0 78-79 31.886117743508727 37.0 33.0 37.0 22.0 37.0 80-81 31.4795050364903 33.0 33.0 37.0 15.0 37.0 82-83 31.146759642765243 33.0 33.0 37.0 15.0 37.0 84-85 30.98525812493642 33.0 33.0 37.0 15.0 37.0 86-87 30.8193730268439 33.0 33.0 37.0 15.0 37.0 88-89 30.613048754320317 33.0 27.0 37.0 15.0 37.0 90-91 30.551880167179164 33.0 27.0 37.0 15.0 37.0 92-93 30.391620781303565 33.0 27.0 37.0 15.0 37.0 94-95 30.250328485342756 33.0 27.0 37.0 15.0 37.0 96-97 30.09610814327347 33.0 27.0 37.0 15.0 37.0 98-99 29.946099338071043 33.0 27.0 37.0 15.0 37.0 100-101 28.738729127182175 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.173300116211351 2306 2 0.23997291546645627 2306 3 0.14817032377763084 2306 4 0.21166720112962167 2306 5 0.28483056710754084 2306 6 0.10411452617994854 2306 7 0.6704043161232889 2306 8 0.012568891910319735 2306 9 0.39314933803723306 2306 10-11 0.3443290195538893 2306 12-13 0.29295612748813227 2306 14-15 0.5867151794366308 2306 16-17 0.5158630386200187 2306 18-19 0.5858369116903148 2306 20-21 0.4754665951136232 2306 22-23 0.38378538814338015 2306 24-25 0.49731146056913644 2306 26-27 0.3217922784985703 2306 28-29 0.40707219184320564 2306 30-31 0.3943533038723075 2306 32-33 0.5339870627228152 2306 34-35 0.4075526014731352 2306 36-37 0.4548821480294194 2306 38-39 0.35528656863188246 2306 40-41 0.421308647956927 2306 42-43 0.3382129589905887 2306 44-45 0.3778048608494444 2306 46-47 0.40690788446902104 2306 48-49 0.37780930742984964 2306 50-51 0.40114789617671676 2306 52-53 0.3345614994525832 2306 54-55 0.46791652405403283 2306 56-57 0.5978222563160216 2306 58-59 0.6729526398950867 2306 60-61 0.4140680639549785 2306 62-63 0.4043347400417403 2306 64-65 0.5025054802977422 2306 66-67 0.5866615732577429 2306 68-69 0.37865357436263025 2306 70-71 0.5294690109310949 2306 72-73 0.17193044686619885 2306 74-75 0.4702499490539793 2306 76-77 0.40045393391813633 2306 78-79 0.46300743165151914 2306 80-81 0.6074079068193932 2306 82-83 0.6606821345308127 2306 84-85 0.5740315632112782 2306 86-87 0.4829916794743987 2306 88-89 0.5500410331651082 2306 90-91 0.23215526806619735 2306 92-93 0.21685207622023484 2306 94-95 0.26203440787170607 2306 96-97 0.3086924489310583 2306 98-99 0.37334662837035637 2306 100-101 0.2883821516401781 2307 1 0.05996455579040827 2307 2 0.022021825148772933 2307 3 0.0232498138837407 2307 4 0.13682422991091414 2307 5 0.10133601555727978 2307 6 0.04769579222467257 2307 7 0.27734000187249563 2307 8 0.003117688873622626 2307 9 0.14555287321130095 2307 10-11 0.2744425464364184 2307 12-13 0.06274240895598382 2307 14-15 0.3320972477787407 2307 16-17 0.365073650692473 2307 18-19 0.34827475929747465 2307 20-21 0.25288651957823305 2307 22-23 0.28592890538065063 2307 24-25 0.40969057419918187 2307 26-27 0.291622883376661 2307 28-29 0.26650489040198266 2307 30-31 0.252095217169817 2307 32-33 0.22354496945697377 2307 34-35 0.1563762843664236 2307 36-37 0.17719434531881006 2307 38-39 -0.029367351233361205 2307 40-41 0.23129481599614365 2307 42-43 0.26302882851818055 2307 44-45 0.1924453963548416 2307 46-47 0.21664023002350064 2307 48-49 0.16274715367237746 2307 50-51 0.0724047923698592 2307 52-53 0.13409113636634373 2307 54-55 0.10159063454268846 2307 56-57 0.1722346845565852 2307 58-59 0.148557160446245 2307 60-61 0.28162158092478506 2307 62-63 0.30930646749206403 2307 64-65 0.3377714125713567 2307 66-67 0.2290121823498268 2307 68-69 0.0574803850039558 2307 70-71 0.2947772360350598 2307 72-73 0.03421755666872173 2307 74-75 0.1568813917100229 2307 76-77 0.28099401903873655 2307 78-79 0.3281351698639199 2307 80-81 0.27046251577174374 2307 82-83 0.16034547857927706 2307 84-85 0.3141926667986894 2307 86-87 0.3442898368323313 2307 88-89 0.5700956022700545 2307 90-91 0.36577643063868237 2307 92-93 0.4972452718125666 2307 94-95 0.2500462081608994 2307 96-97 0.2207399557665255 2307 98-99 0.4785316212933495 2307 100-101 0.35780096365269287 2201 1 0.3700642845695725 2201 2 0.20085108513681504 2201 3 0.27617137954565507 2201 4 0.3762407211478376 2201 5 0.39293212636657415 2201 6 0.3130124547219779 2201 7 0.6699518827456572 2201 8 0.3435902987969328 2201 9 0.5133722175904367 2201 10-11 0.4458254030616189 2201 12-13 0.4357768778731028 2201 14-15 0.7248137747567469 2201 16-17 0.6184337147712924 2201 18-19 0.5458400973837882 2201 20-21 0.3290770837795307 2201 22-23 0.3158833322623096 2201 24-25 0.7332994122655805 2201 26-27 0.5657252631904797 2201 28-29 0.6859081264656268 2201 30-31 0.5622248820237488 2201 32-33 0.6236216058083741 2201 34-35 0.5679142723342068 2201 36-37 0.5416971630843861 2201 38-39 0.4788675423074551 2201 40-41 0.47308901251059865 2201 42-43 0.6159197031956225 2201 44-45 0.5743380799521418 2201 46-47 0.5081635831554223 2201 48-49 0.5720679197547511 2201 50-51 0.4949172151901067 2201 52-53 0.6410260233212242 2201 54-55 0.7958879945193047 2201 56-57 0.6322097621235585 2201 58-59 0.7410165495576919 2201 60-61 0.7153705744869683 2201 62-63 0.6972174735290011 2201 64-65 0.6416794499909244 2201 66-67 0.6900974978200907 2201 68-69 0.6080558214393861 2201 70-71 0.5710072898236831 2201 72-73 0.4976594920073154 2201 74-75 0.5748349408139006 2201 76-77 0.6148939685118435 2201 78-79 0.5890546134486101 2201 80-81 0.7498999466421914 2201 82-83 0.4951278914737607 2201 84-85 0.6383917661653733 2201 86-87 0.7464919344921377 2201 88-89 0.898357807923599 2201 90-91 0.7252762795265824 2201 92-93 0.6704324928050944 2201 94-95 0.5922319425019289 2201 96-97 0.5407261383156658 2201 98-99 0.5910299585396324 2201 100-101 0.2426436521829345 1101 1 0.058830738987122544 1101 2 -0.06405590596956401 1101 3 -0.1341106726761545 1101 4 -0.03261470187563731 1101 5 0.023583760819398947 1101 6 0.06065416584926453 1101 7 0.3395922310705686 1101 8 -0.05422183742446407 1101 9 0.12292484891349886 1101 10-11 -0.008573199928818553 1101 12-13 0.06027196657367995 1101 14-15 0.25312109492031 1101 16-17 0.2651301905583594 1101 18-19 0.18489530583944713 1101 20-21 0.15245704809135674 1101 22-23 0.08817902597135685 1101 24-25 0.19717704627510102 1101 26-27 0.04738714409212008 1101 28-29 0.1873914767654341 1101 30-31 0.2077625020761431 1101 32-33 0.24357925571477068 1101 34-35 0.21799668622471557 1101 36-37 0.2689065621822877 1101 38-39 0.15891191192159226 1101 40-41 0.23808526143819364 1101 42-43 0.2723405443483671 1101 44-45 0.37717827379618996 1101 46-47 0.17441624811223022 1101 48-49 0.09895297388164437 1101 50-51 0.09203383851188107 1101 52-53 0.16928210750081973 1101 54-55 0.25075195986532606 1101 56-57 0.38432271664968454 1101 58-59 0.3222744515550957 1101 60-61 0.39380867730449864 1101 62-63 0.44975053819130295 1101 64-65 0.3761983914729541 1101 66-67 0.35889874782850484 1101 68-69 0.20789605492588237 1101 70-71 0.22547979230631654 1101 72-73 0.2089155515038854 1101 74-75 0.4951169855560096 1101 76-77 0.5020602396267364 1101 78-79 0.3585320787159674 1101 80-81 0.5796878994791577 1101 82-83 0.42983342668036073 1101 84-85 0.37983741376203284 1101 86-87 0.3261665074570388 1101 88-89 0.5831854493255655 1101 90-91 0.4157013586805647 1101 92-93 0.46425789602979606 1101 94-95 0.41945871944937707 1101 96-97 0.29060141422331043 1101 98-99 0.3347142820488713 1101 100-101 0.33369012035316814 2304 1 0.279559144670106 2304 2 0.18603036922643668 2304 3 0.2378350638709925 2304 4 0.49000884177474546 2304 5 0.35106010197339543 2304 6 0.22118435730728692 2304 7 0.5104317008791099 2304 8 0.30440106463009897 2304 9 0.40518326621325684 2304 10-11 0.3459230744408899 2304 12-13 0.39897866493129186 2304 14-15 0.6162400283158576 2304 16-17 0.5263763446288507 2304 18-19 0.49713131589111725 2304 20-21 0.38749984544914895 2304 22-23 0.354650297546236 2304 24-25 0.5635379828105869 2304 26-27 0.48278767972742287 2304 28-29 0.4122392192716404 2304 30-31 0.53044145627495 2304 32-33 0.544217935193906 2304 34-35 0.4331354313216096 2304 36-37 0.5490846536291301 2304 38-39 0.5005835606770432 2304 40-41 0.4333685600184154 2304 42-43 0.4096882260973942 2304 44-45 0.38923249836444995 2304 46-47 0.3558201764284803 2304 48-49 0.5172953235805267 2304 50-51 0.4576681191711671 2304 52-53 0.5039982742574054 2304 54-55 0.6367982228126792 2304 56-57 0.6642577793753972 2304 58-59 0.6079036772169815 2304 60-61 0.6217598965795972 2304 62-63 0.5551666762705025 2304 64-65 0.6722608401331343 2304 66-67 0.6542855766439359 2304 68-69 0.6005186750671285 2304 70-71 0.6162578244541592 2304 72-73 0.6566157707157885 2304 74-75 0.5852587685119204 2304 76-77 0.5989606513173911 2304 78-79 0.510087219502509 2304 80-81 0.6355966567760909 2304 82-83 0.7514114607724274 2304 84-85 0.6888630144075165 2304 86-87 0.5388287898855744 2304 88-89 0.5068185342835427 2304 90-91 0.7120068360618816 2304 92-93 0.6907849669990433 2304 94-95 0.5462856878605002 2304 96-97 0.6935770578848768 2304 98-99 0.5325566730626399 2304 100-101 0.5836743234862993 2202 1 0.22858230183346961 2202 2 0.1393575381126979 2202 3 0.011542391073835034 2202 4 0.11149285404617615 2202 5 0.1609143363085792 2202 6 0.18411800722154936 2202 7 0.4103129830318579 2202 8 0.11392030305968603 2202 9 0.1893048520956242 2202 10-11 0.2199606958524214 2202 12-13 0.17350846258854347 2202 14-15 0.2502938967022956 2202 16-17 0.23489725607891643 2202 18-19 0.24358414676976992 2202 20-21 0.17893126401641268 2202 22-23 0.1861868643410176 2202 24-25 0.34322005667685573 2202 26-27 0.2745312387291463 2202 28-29 0.33846469698363535 2202 30-31 0.2894847131926426 2202 32-33 0.33228208984839114 2202 34-35 0.33980282936335726 2202 36-37 0.222805824717355 2202 38-39 0.18014887573644955 2202 40-41 0.3911887880245999 2202 42-43 0.2217868676987962 2202 44-45 0.4035498185016877 2202 46-47 0.27226090043755846 2202 48-49 0.3435542355817418 2202 50-51 0.3241180506700587 2202 52-53 0.18832013936833647 2202 54-55 0.40865621589334467 2202 56-57 0.4230695430210929 2202 58-59 0.43925674722916597 2202 60-61 0.4132295164343347 2202 62-63 0.32787130130363806 2202 64-65 0.3791792565493708 2202 66-67 0.38861390658812667 2202 68-69 0.4836077160646184 2202 70-71 0.5963020595860229 2202 72-73 0.4286771774896323 2202 74-75 0.6214311431692465 2202 76-77 0.46937339500569664 2202 78-79 0.4977086963316282 2202 80-81 0.5019861845298159 2202 82-83 0.47826670466642796 2202 84-85 0.5002710942736748 2202 86-87 0.5815737450300809 2202 88-89 0.7023255404649724 2202 90-91 0.6482061148738154 2202 92-93 0.5888942971247744 2202 94-95 0.48676514340399635 2202 96-97 0.39895762759994113 2202 98-99 0.39501832484712907 2202 100-101 0.6330620580650574 1102 1 0.006307231566708538 1102 2 -0.268150351043392 1102 3 -0.24751745489736976 1102 4 -0.4128354261685274 1102 5 -0.2113016287051721 1102 6 -0.014163628040698484 1102 7 -0.09991628446887546 1102 8 -0.15512909168223388 1102 9 -0.2898852791028119 1102 10-11 -0.2649598545113463 1102 12-13 -0.33014946766218856 1102 14-15 -0.24960019581644843 1102 16-17 -0.3194941627515604 1102 18-19 -0.17725349896399933 1102 20-21 -0.2444430878551671 1102 22-23 -0.07582063416231932 1102 24-25 -0.16622029521501958 1102 26-27 -0.034985594852777524 1102 28-29 -0.20983672458648783 1102 30-31 -0.050500497810567424 1102 32-33 -0.2623636845065178 1102 34-35 -0.18880677663545242 1102 36-37 -0.206047178235373 1102 38-39 -0.1786225467468796 1102 40-41 -0.2108191417187868 1102 42-43 -0.23765246951062835 1102 44-45 -0.15224376464647094 1102 46-47 -0.2586418879098247 1102 48-49 -0.1524631357818933 1102 50-51 -0.1857738349143503 1102 52-53 -0.21567314343381128 1102 54-55 0.03459226045819719 1102 56-57 0.12507316036404603 1102 58-59 -0.04067917557378564 1102 60-61 -0.14369226676861047 1102 62-63 -0.002045088862054456 1102 64-65 0.011802692670030979 1102 66-67 0.06993449043641675 1102 68-69 0.055912085287275204 1102 70-71 0.06355001246416947 1102 72-73 0.1910017642602071 1102 74-75 0.40443876343448437 1102 76-77 0.29357585459596436 1102 78-79 0.15851366929034327 1102 80-81 0.23382907617187598 1102 82-83 0.11126303658935299 1102 84-85 0.25622284104952087 1102 86-87 0.08393858932705811 1102 88-89 0.35272304231676443 1102 90-91 0.1458120984562541 1102 92-93 0.18017449661134322 1102 94-95 0.19464186963252317 1102 96-97 0.204976343417826 1102 98-99 0.2189709188828246 1102 100-101 0.2018809930543526 2305 1 0.16601947980479181 2305 2 0.06651485710206018 2305 3 0.08097408901970837 2305 4 0.010821843613356918 2305 5 0.035667207929321876 2305 6 0.3272778585375704 2305 7 0.13020301365953912 2305 8 0.2924379056568043 2305 9 0.24680181799461565 2305 10-11 0.19933281836795658 2305 12-13 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-1.1185932036568857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1710.0 3 254.0 4 284.0 5 227.0 6 386.0 7 2731.0 8 14903.0 9 41653.0 10 49191.0 11 32407.0 12 18973.0 13 12537.0 14 9422.0 15 7969.0 16 7299.0 17 7180.0 18 7350.0 19 7895.0 20 8723.0 21 9523.0 22 10599.0 23 11346.0 24 12737.0 25 14897.0 26 17272.0 27 20876.0 28 26087.0 29 31858.0 30 38842.0 31 50542.0 32 69340.0 33 98719.0 34 149852.0 35 253914.0 36 479076.0 37 677944.0 38 134920.0 39 88.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.064210795161394 23.579683265597172 22.286253987462953 14.069851951778482 2 32.383854155062224 24.99657222577378 30.013976749407423 12.605596869756578 3 36.63063502186784 19.939761177740934 27.713670280681903 15.715933519709324 4 30.16985045244624 17.53939147843652 32.67027664198622 19.62048142713101 5 34.12574926230435 16.042524170028134 32.03029949929229 17.80142706837522 6 31.15515888945105 16.48348729539853 36.20649695210375 16.154856863046664 7 36.101776366453855 18.2751076553282 27.986958864400112 17.63615711381783 8 29.679277566132807 29.701291498296488 23.25330797876555 17.366122956805153 9 31.466144598898055 26.862895977397617 23.461833429037192 18.209125994667133 10-11 26.63389003372571 29.06614854296977 27.652832861007866 16.647128562296654 12-13 28.28953426272076 24.511647607671794 27.488379801990888 19.710438327616565 14-15 25.80050427995568 24.68558519494007 29.161813908919115 20.35209661618514 16-17 26.793386776851364 25.558498467979607 29.868262734484556 17.77985202068447 18-19 26.30782888051364 25.72564852407966 29.94244144044995 18.024081154956757 20-21 24.993255245491934 27.80654588060371 29.005334993756282 18.19486388014807 22-23 27.551964190424165 25.56295476091453 28.491468961902022 18.39361208675928 24-25 24.41739580756864 28.29747317036304 28.659832151592603 18.62529887047572 26-27 22.594620416277518 29.82844783151987 29.029091741773804 18.547840010428807 28-29 23.367326733521974 29.042477320827466 28.41462661977252 19.175569325878044 30-31 25.50427017793283 27.251580997724272 28.159086228427032 19.08506259591586 32-33 22.83591769353222 27.03168230861174 29.73328116373927 20.39911883411677 34-35 20.520082256814142 26.99465485068227 33.55110264607617 18.93416024642742 36-37 21.38880689147036 27.788957295101312 30.363869171993286 20.458366641435045 38-39 20.448197951414883 27.358211205098883 28.243834335168323 23.949756508317915 40-41 22.100635199332334 26.55194108698813 29.40866548956152 21.93875822411802 42-43 23.96998233939803 26.74451803803218 30.416863568900265 18.868636053669523 44-45 21.927355283448037 28.555299488277647 30.832713775373556 18.684631452900767 46-47 23.28612921278061 29.105811488531007 29.049785150146086 18.558274148542296 48-49 20.776790303802827 28.923103910221094 30.479806844629287 19.82029894134679 50-51 19.875062578101975 27.708232253879846 30.8544254247053 21.562279743312885 52-53 24.193508606714097 27.0619557736649 28.318889260871984 20.425646358749024 54-55 25.87545421856396 26.908757897833496 28.262231122015002 18.953556761587546 56-57 26.294403177960195 26.928279255530217 27.38523973050829 19.392077836001295 58-59 24.53882982921498 26.893879602908527 27.78184075163253 20.785449816243965 60-61 21.16393801472428 27.183552235087642 27.558916520466553 24.093593229721527 62-63 23.390131541718326 27.305559699636976 27.77080428903853 21.533504469606164 64-65 21.50082401140068 28.475210204365116 29.680830630930355 20.343135153303848 66-67 20.826563323026555 28.303141534894877 28.61770449432927 22.252590647749297 68-69 20.95040542684522 27.029385406348755 32.49167063303189 19.528538533774135 70-71 20.394036973439714 30.96261394377998 29.77031300337874 18.87303607940156 72-73 20.17141667740263 33.96655138995504 27.0217386597705 18.84029327287183 74-75 20.90996724394015 33.405864965978154 26.58394016322511 19.100227626856583 76-77 20.907735734674855 32.71035389890734 26.85425432394959 19.527656042468212 78-79 20.799501289756783 32.44564167677599 27.394871370529234 19.359985662937987 80-81 19.8759539141004 32.55946036865203 27.58400222294977 19.980583494297797 82-83 19.293343535075063 32.055156311384145 27.96282254287562 20.688677610665167 84-85 19.44892010246386 31.50988807114316 28.281281561608907 20.75991026478407 86-87 19.441765753224026 31.48442524221337 29.01499716834282 20.05881183621978 88-89 19.864571533089787 31.673874665519048 28.766809282761763 19.6947445186294 90-91 20.55935424067886 30.924491057655125 28.006427752524665 20.509726949141353 92-93 20.034992943968422 31.654510801815533 27.654649693324117 20.655846560891924 94-95 19.779206879351335 31.715930085822546 27.946914647538495 20.557948387287624 96-97 20.285975955694795 32.259989364796276 27.342290023848832 20.111744655660097 98-99 20.70434283580333 31.40878642798695 27.216013318707223 20.670857417502496 100-101 20.742429427520886 31.412890493331926 26.831715310280472 21.012964768866716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1213.0 1 757.5 2 246.5 3 162.5 4 134.5 5 140.0 6 184.5 7 259.5 8 357.0 9 486.5 10 590.5 11 704.5 12 772.0 13 748.0 14 676.0 15 572.5 16 498.0 17 467.5 18 479.0 19 480.5 20 520.5 21 632.5 22 733.5 23 868.5 24 1113.5 25 1404.0 26 1916.0 27 2711.0 28 3474.5 29 4268.5 30 5298.0 31 6943.5 32 11099.0 33 19458.5 34 33833.0 35 54266.5 36 82060.0 37 116265.5 38 145975.5 39 162068.5 40 165412.0 41 153220.0 42 142529.5 43 145312.5 44 141626.5 45 132402.0 46 127569.0 47 114883.5 48 94042.5 49 79172.5 50 68785.0 51 58643.5 52 48752.0 53 41582.5 54 34801.5 55 26751.0 56 20907.0 57 16958.5 58 13792.0 59 10989.5 60 8587.0 61 6724.0 62 5101.5 63 3871.5 64 3049.0 65 2346.0 66 1754.0 67 1376.0 68 1115.0 69 862.5 70 609.5 71 428.5 72 313.5 73 212.0 74 153.5 75 128.5 76 98.5 77 70.0 78 62.5 79 52.0 80 43.5 81 33.5 82 26.0 83 21.0 84 19.5 85 18.0 86 15.0 87 13.5 88 12.5 89 11.0 90 9.0 91 7.5 92 3.5 93 1.0 94 0.5 95 2.0 96 3.0 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2548379458061163 2 0.24150191107087504 3 0.2059818954779729 4 0.20876023604781482 5 0.22354955661958875 6 0.2262424097872817 7 0.2034600171145779 8 0.19837351668671346 9 0.1793953134096394 10-11 0.16751256451093083 12-13 0.195894382024393 14-15 0.19292369479971583 16-17 0.19394954362550362 18-19 0.18546491896221715 20-21 0.1871319233041223 22-23 0.1544971075337483 24-25 0.4598367361593758 26-27 0.3216036068844715 28-29 0.27048214039938 30-31 0.2746923949552174 32-33 0.3158118353888779 34-35 0.3127984044631263 36-37 0.3049976790170317 38-39 0.33004548784668347 40-41 0.3341275112992974 42-43 0.36894225582447043 44-45 0.33726917332827244 46-47 0.36244521326114776 48-49 0.3390216650723266 50-51 0.3607140933676309 52-53 0.34953661553665144 54-55 0.3264122732553517 56-57 0.3620818918020146 58-59 0.34938701258289073 60-61 0.3159400664921014 62-63 0.29416215079464814 64-65 0.3006591933579708 66-67 0.3095712550320022 68-69 0.3169872871684264 70-71 0.2928584679118762 72-73 0.30016764079561414 74-75 0.29837240535048554 76-77 0.30142857997731165 78-79 0.3038863427890949 80-81 0.32012894919740154 82-83 0.33758975108633116 84-85 0.3324177632563177 86-87 0.3356449126874418 88-89 0.3309003618681733 90-91 0.30519002567186687 92-93 0.2893748562743051 94-95 0.29837240535048554 96-97 0.2867033749571494 98-99 0.30636547745141535 100-101 0.3277586998391982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.466767431797635 #Duplication Level Percentage of deduplicated Percentage of total 1 87.24479212042598 37.05004296613661 2 5.301632422730921 4.502863822099837 3 2.2251173676933855 2.8348062528686624 4 1.053090247282019 1.7888535446407905 5 0.7060230648927479 1.4991258649142647 6 0.5040900804988554 1.2844245727932624 7 0.26416717792340166 0.7852828275591183 8 0.2637756425626679 0.896135909350544 9 0.22347490421253283 0.8541231105633197 >10 1.7214644444704628 14.382211151299046 >50 0.24581761497284021 7.300587832681636 >100 0.22798475437223972 19.841433980813044 >500 0.014278357055431384 3.9488346073576532 >1k 0.004291800906391889 3.031273556922156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3551 0.1517828825155181 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3234 0.13823312927490441 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3121 0.1334030910534869 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2848 0.12173406066015081 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2825 0.12075095553543752 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2667 0.11399745076566793 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2659 0.11365550115707199 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2521 0.10775687040879221 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT 2452 0.10480755503465232 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003846933096704204 0.0 0.0 2 0.0042316264063746245 0.0 0.0 3 0.004830038221417501 0.0 0.0 4 0.005129244128938939 0.0 0.0 5 0.006668017367620621 0.0 0.0 6 0.008335021709525776 0.0 0.0 7 0.009916538649281948 0.0 0.0 8 0.011241593382591175 0.0 0.0 9 0.012823110322347347 0.0 0.0 10-11 0.018956831426536827 0.0 0.0 12-13 0.0244280251640717 0.0 0.0 14-15 0.03060448996933567 0.0 0.0 16-17 0.035605502995051136 0.0 0.0 18-19 0.04043554121646863 0.0 0.0 20-21 0.04639828751636015 0.0 0.0 22-23 0.0524678930689379 0.0 0.0 24-25 0.05939237264300547 0.0 0.0 26-27 0.06608176186116332 0.0 0.0 28-29 0.07358328139973652 0.0 0.0 30-31 0.13036828827719804 0.0 0.0 32-33 0.1421228060726831 0.0 0.0 34-35 0.15248815358324722 0.0 0.0 36-37 0.16362288771315214 0.0 0.0 38-39 0.17576209881830762 0.0 0.0 40-41 0.18984614832235247 0.0 0.0 42-43 0.20651619174140404 0.0 0.0 44-45 0.22613555053459547 0.0 0.0 46-47 0.24710133591163339 0.0 0.0 48-49 0.27035390929615655 0.0 0.0 50-51 0.29642756695159617 0.0 0.0 52-53 0.325044474820968 0.0 0.0 54-55 0.3568244165698522 0.0 0.0 56-57 0.3900362723047318 0.0 0.0 58-59 0.4267958552287942 0.0 0.0 60-61 0.4653079298969107 0.0 0.0 62-63 0.5099109819681422 0.0 0.0 64-65 0.5559032043242947 0.0 0.0 66-67 0.6015534770718514 0.0 0.0 68-69 0.6514994917773942 0.0 0.0 70-71 0.7052069521774924 0.0 0.0 72-73 0.7647275559237213 0.0 0.0 74-75 0.8265563195279728 0.0 0.0 76-77 0.8907787303923957 0.0 0.0 78-79 0.9595747172717892 0.0 0.0 80-81 1.0322817527994987 0.0 0.0 82-83 1.1123407049120206 0.0 0.0 84-85 1.2036198785565966 0.0 0.0 86-87 1.3009900296042873 0.0 0.0 88-89 1.4050923135712106 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 2245 0.0 83.47005 4 GAGCGGC 1670 0.0 82.351845 9 ATTCGGA 325 0.0 81.75076 2 AGAGCGG 1765 0.0 80.8766 8 CGGAAAG 1220 0.0 78.545784 5 GATCCGG 515 0.0 78.31676 1 TCGGGAG 2390 0.0 76.422646 3 GAGAGCG 2295 0.0 73.77748 7 GAACCGG 415 0.0 73.17704 1 TCGGAGA 555 0.0 72.65673 4 GGATCGG 910 0.0 72.4797 1 AGTCGCG 20 0.002083962 71.16696 2 AAGCGGC 690 0.0 70.79131 9 AACCGGA 410 0.0 70.58836 2 ACCGGAA 450 0.0 69.58099 3 GTTCGGA 275 0.0 69.01039 2 TCGGGAA 740 0.0 67.95687 3 AATCGGA 505 0.0 67.643845 2 ATCGGGA 3340 0.0 64.63267 2 AAGCGTC 295 0.0 64.30279 9 >>END_MODULE