FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339526
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA35510.1517828825155181No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32340.13823312927490441No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31210.1334030910534869No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28480.12173406066015081No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC28250.12075095553543752No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC26670.11399745076566793No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT26590.11365550115707199No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT25210.10775687040879221No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT24520.10480755503465232Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA22450.083.470054
GAGCGGC16700.082.3518459
ATTCGGA3250.081.750762
AGAGCGG17650.080.87668
CGGAAAG12200.078.5457845
GATCCGG5150.078.316761
TCGGGAG23900.076.4226463
GAGAGCG22950.073.777487
GAACCGG4150.073.177041
TCGGAGA5550.072.656734
GGATCGG9100.072.47971
AGTCGCG200.00208396271.166962
AAGCGGC6900.070.791319
AACCGGA4100.070.588362
ACCGGAA4500.069.580993
GTTCGGA2750.069.010392
TCGGGAA7400.067.956873
AATCGGA5050.067.6438452
ATCGGGA33400.064.632672
AAGCGTC2950.064.302799