##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34042-1.34200000035847.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5394624 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.018873975276126 33.0 33.0 33.0 27.0 33.0 2 30.499293185215503 33.0 33.0 33.0 22.0 33.0 3 30.346746687072166 33.0 33.0 33.0 22.0 33.0 4 33.6572899241912 37.0 37.0 37.0 22.0 37.0 5 33.71559927068133 37.0 37.0 37.0 22.0 37.0 6 34.36856822644173 37.0 37.0 37.0 27.0 37.0 7 33.73623944875491 37.0 37.0 37.0 22.0 37.0 8 34.12384180992039 37.0 37.0 37.0 27.0 37.0 9 33.4073544328576 37.0 37.0 37.0 15.0 37.0 10-11 33.447555195691116 37.0 37.0 37.0 15.0 37.0 12-13 33.45814648064444 37.0 37.0 37.0 18.5 37.0 14-15 35.02262270734717 40.0 37.0 40.0 15.0 40.0 16-17 34.94047156947361 40.0 37.0 40.0 15.0 40.0 18-19 35.013180158617175 40.0 37.0 40.0 18.5 40.0 20-21 34.99799309460678 40.0 37.0 40.0 15.0 40.0 22-23 35.865442892776215 40.0 37.0 40.0 27.0 40.0 24-25 35.16095607404705 40.0 37.0 40.0 22.0 40.0 26-27 35.03923869392936 40.0 37.0 40.0 21.0 40.0 28-29 34.661020119289134 40.0 37.0 40.0 15.0 40.0 30-31 34.653398457427244 40.0 37.0 40.0 15.0 40.0 32-33 34.427227087559764 40.0 37.0 40.0 15.0 40.0 34-35 34.710995706095545 37.0 37.0 40.0 18.5 40.0 36-37 34.39467069438018 37.0 37.0 40.0 15.0 40.0 38-39 34.33558251696504 37.0 37.0 40.0 15.0 40.0 40-41 34.18023239432442 37.0 37.0 40.0 15.0 40.0 42-43 34.0457162538112 37.0 37.0 40.0 15.0 40.0 44-45 33.909355591789165 37.0 33.0 40.0 15.0 40.0 46-47 33.859207055023674 37.0 33.0 40.0 15.0 40.0 48-49 33.755489446530476 37.0 33.0 40.0 15.0 40.0 50-51 32.808691671560425 37.0 33.0 38.5 15.0 38.5 52-53 33.24836689266944 37.0 33.0 38.5 15.0 40.0 54-55 33.84633155155948 37.0 33.0 40.0 15.0 40.0 56-57 34.164195688151764 37.0 33.0 40.0 18.5 40.0 58-59 33.56631991404777 37.0 33.0 40.0 15.0 40.0 60-61 33.36013742570381 37.0 33.0 40.0 15.0 40.0 62-63 32.95376619389971 37.0 33.0 40.0 10.5 40.0 64-65 32.73673531278547 37.0 33.0 37.0 10.5 40.0 66-67 32.60309430277254 37.0 33.0 37.0 15.0 40.0 68-69 32.715139831802915 37.0 33.0 37.0 15.0 40.0 70-71 32.281656701189924 37.0 33.0 37.0 15.0 38.5 72-73 32.60785339256267 37.0 33.0 37.0 18.5 37.0 74-75 32.71812910408585 37.0 33.0 37.0 22.0 37.0 76-77 32.428357008755384 37.0 33.0 37.0 22.0 37.0 78-79 31.95618350787747 37.0 33.0 37.0 22.0 37.0 80-81 31.46042087455956 37.0 33.0 37.0 15.0 37.0 82-83 31.05505304910963 33.0 33.0 37.0 15.0 37.0 84-85 30.87809215248366 33.0 33.0 37.0 15.0 37.0 86-87 30.710636274187042 33.0 33.0 37.0 15.0 37.0 88-89 30.51623254929352 33.0 30.0 37.0 15.0 37.0 90-91 30.445636804344474 33.0 27.0 37.0 15.0 37.0 92-93 30.28904553866961 33.0 27.0 37.0 15.0 37.0 94-95 30.161005567765244 33.0 27.0 37.0 15.0 37.0 96-97 30.024707838766894 33.0 27.0 37.0 10.5 37.0 98-99 29.894072969682412 33.0 27.0 37.0 6.0 37.0 100-101 28.738928514758395 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.10436765611512655 2306 2 0.064586367333753 2306 3 -0.06641005420558344 2306 4 0.21727320283834217 2306 5 0.1494291865531281 2306 6 -0.06437209163252788 2306 7 0.5379437544349415 2306 8 -0.1633545869786701 2306 9 0.23359945901236046 2306 10-11 0.08909242271626994 2306 12-13 0.0283426115753187 2306 14-15 0.3380984773804343 2306 16-17 0.3439207202310399 2306 18-19 0.37195367515023037 2306 20-21 0.3072482734607078 2306 22-23 0.09356753880779678 2306 24-25 0.5211825715300691 2306 26-27 0.0914310699804588 2306 28-29 0.28565204842531244 2306 30-31 0.30099398384031417 2306 32-33 0.39673435972000703 2306 34-35 0.18022917820681528 2306 36-37 0.517444438525942 2306 38-39 0.3280678173910516 2306 40-41 0.2939208261677777 2306 42-43 0.3506944318982832 2306 44-45 0.3919195792042771 2306 46-47 0.3575906564332598 2306 48-49 0.26442456333806064 2306 50-51 0.32686001963433853 2306 52-53 0.1549430906483238 2306 54-55 0.32998075850391473 2306 56-57 0.3034816730679495 2306 58-59 0.4796375440365708 2306 60-61 0.34887709031351477 2306 62-63 0.419744324334566 2306 64-65 0.41144124408297245 2306 66-67 0.28230397504808735 2306 68-69 0.23220347493407445 2306 70-71 0.37923623657452765 2306 72-73 0.009352311959268889 2306 74-75 0.08716460619974242 2306 76-77 0.24641407417697536 2306 78-79 0.40856694563084517 2306 80-81 0.4722251468580474 2306 82-83 0.5607546618780006 2306 84-85 0.5416389755526971 2306 86-87 0.5719120224601788 2306 88-89 0.6117365311725358 2306 90-91 0.4761941107170884 2306 92-93 0.37570089038635146 2306 94-95 0.2640040687439189 2306 96-97 0.2708621918831362 2306 98-99 0.3432642564103112 2306 100-101 0.09215824792838845 2307 1 0.11629848561057443 2307 2 0.028381382361068574 2307 3 -0.03795542572798993 2307 4 0.18639061951596148 2307 5 0.13594327424204522 2307 6 0.02301948499454909 2307 7 0.5464966112690632 2307 8 -0.10626315284439869 2307 9 0.19859177929063065 2307 10-11 0.08889215935515438 2307 12-13 0.12418849178639135 2307 14-15 0.3992697266910241 2307 16-17 0.382273192691045 2307 18-19 0.31100070961485926 2307 20-21 0.28990859036481 2307 22-23 0.1530588119947751 2307 24-25 0.41373651011517865 2307 26-27 0.15987237255912135 2307 28-29 0.21468430351966816 2307 30-31 0.3160335978404163 2307 32-33 0.3430471396530024 2307 34-35 0.10057694194576783 2307 36-37 0.4231238811400573 2307 38-39 0.1546464221085344 2307 40-41 0.17005800308444208 2307 42-43 0.21213767107955306 2307 44-45 0.24014925109199936 2307 46-47 0.21880203076079852 2307 48-49 0.09515960287576775 2307 50-51 0.11493646407247127 2307 52-53 0.13541734717141196 2307 54-55 0.16901386989678002 2307 56-57 0.059217163116009885 2307 58-59 0.2096189027731583 2307 60-61 0.2533915526165842 2307 62-63 0.2967669678398721 2307 64-65 0.2185923955019291 2307 66-67 0.2336479691005664 2307 68-69 0.14325517129298504 2307 70-71 0.2031319041644437 2307 72-73 -0.1950272340025876 2307 74-75 0.031244334562394727 2307 76-77 0.1285409670932225 2307 78-79 0.35927177401007526 2307 80-81 0.28872629536349237 2307 82-83 0.2739684198985657 2307 84-85 0.33637432472584194 2307 86-87 0.46628746976434954 2307 88-89 0.47536375904487826 2307 90-91 0.3586286989157692 2307 92-93 0.3933310046818157 2307 94-95 0.23735536198485363 2307 96-97 0.3404345385185614 2307 98-99 0.394206059811804 2307 100-101 0.25431474693812106 2201 1 0.11018164805046737 2201 2 0.02483075897690412 2201 3 0.031053347326874814 2201 4 0.23859299604854556 2201 5 0.17106474471314215 2201 6 0.025560385790853957 2201 7 0.5705313839774107 2201 8 -0.04445350495913658 2201 9 0.23170843009424402 2201 10-11 0.18007151533777233 2201 12-13 0.14569755172622223 2201 14-15 0.42544741761578564 2201 16-17 0.30377387108962495 2201 18-19 0.2485219447277771 2201 20-21 0.21336008655431016 2201 22-23 0.11641713244640783 2201 24-25 0.31555237946663794 2201 26-27 0.24491801539521418 2201 28-29 0.19867190333875584 2201 30-31 0.3199073887046495 2201 32-33 0.3691173893441757 2201 34-35 0.08749779994558793 2201 36-37 0.3172526396144164 2201 38-39 0.23811785399079355 2201 40-41 0.2920833997169794 2201 42-43 0.35271186853291425 2201 44-45 0.4261845908200499 2201 46-47 0.24676262739153287 2201 48-49 0.33651339617008347 2201 50-51 0.27802213129940156 2201 52-53 0.23967415759558008 2201 54-55 0.4638331773677109 2201 56-57 0.4278401602651627 2201 58-59 0.4830602173588403 2201 60-61 0.3594748640116521 2201 62-63 0.5419290184711301 2201 64-65 0.4892129558164058 2201 66-67 0.35887157062071395 2201 68-69 0.3205838334639992 2201 70-71 0.35844636384192086 2201 72-73 0.1520290449531032 2201 74-75 0.32324176272302196 2201 76-77 0.4179946818598168 2201 78-79 0.285513929674984 2201 80-81 0.5653579395631247 2201 82-83 0.4871278896901394 2201 84-85 0.4027673818845763 2201 86-87 0.3978372786202051 2201 88-89 0.4880297304373862 2201 90-91 0.42232363106466053 2201 92-93 0.3223027864512318 2201 94-95 0.2744698392129372 2201 96-97 0.25636008568132596 2201 98-99 0.24831324003631394 2201 100-101 0.2870680101140195 1101 1 0.010382500976053421 1101 2 -0.1696965582798029 1101 3 -0.06849053749879985 1101 4 -0.05735770546806407 1101 5 0.034937233876739526 1101 6 -0.07654346468281403 1101 7 0.36517570225936424 1101 8 -0.13707313903196194 1101 9 0.003334737351664785 1101 10-11 0.013104128761625589 1101 12-13 -0.02095722601378469 1101 14-15 0.15832683872296371 1101 16-17 0.08027726083241049 1101 18-19 -0.016395336316655573 1101 20-21 0.020768741133430524 1101 22-23 0.05638032572819185 1101 24-25 0.13186653945802362 1101 26-27 0.03412419875603234 1101 28-29 0.11581884767480943 1101 30-31 0.21470629800052876 1101 32-33 0.17888354814387952 1101 34-35 -0.04912310134695019 1101 36-37 0.19959772340337167 1101 38-39 -0.016414799295937144 1101 40-41 0.11242370975221405 1101 42-43 0.1000851150910691 1101 44-45 0.22978476761539213 1101 46-47 0.052691984788054924 1101 48-49 0.03788436425040942 1101 50-51 0.08227794743226013 1101 52-53 0.1734874538914042 1101 54-55 0.22981609088206056 1101 56-57 0.32019152420478747 1101 58-59 0.3608444990788513 1101 60-61 0.2734745646904386 1101 62-63 0.3830259957465003 1101 64-65 0.32033372493204837 1101 66-67 0.22489665485725396 1101 68-69 0.10118907773948393 1101 70-71 0.31835410577108547 1101 72-73 0.07499736725771555 1101 74-75 0.4050013868091469 1101 76-77 0.3609696200024288 1101 78-79 0.27486127655610204 1101 80-81 0.47778148327451575 1101 82-83 0.49426823916863327 1101 84-85 0.4207916940402363 1101 86-87 0.3256033073410549 1101 88-89 0.5169574121945963 1101 90-91 0.38710740645626274 1101 92-93 0.4372456286879718 1101 94-95 0.4315008805010798 1101 96-97 0.3095541157617099 1101 98-99 0.32353412930005376 1101 100-101 0.2957262957867428 2304 1 0.15907115107775027 2304 2 -0.0016392685289829956 2304 3 -0.04353895204040725 2304 4 0.20375351039607636 2304 5 0.11128765044047384 2304 6 0.15818053434607293 2304 7 0.5321072520498831 2304 8 0.1468331525308031 2304 9 0.26242462812692935 2304 10-11 0.20682458774182066 2304 12-13 0.25972841593603135 2304 14-15 0.3846974850495073 2304 16-17 0.3497958393501577 2304 18-19 0.34539788567948193 2304 20-21 0.27275248829271703 2304 22-23 0.31667460113143164 2304 24-25 0.4875244120902025 2304 26-27 0.32742060504892834 2304 28-29 0.283004840795833 2304 30-31 0.42907427131386555 2304 32-33 0.3971071608471419 2304 34-35 0.2306697518475076 2304 36-37 0.493784787881566 2304 38-39 0.26320963233454364 2304 40-41 0.30525249852632186 2304 42-43 0.3256033469650603 2304 44-45 0.3153027874976573 2304 46-47 0.2162437047493313 2304 48-49 0.37890775055930703 2304 50-51 0.37031067384075556 2304 52-53 0.3834497545193116 2304 54-55 0.49883624916424907 2304 56-57 0.4343192650899752 2304 58-59 0.542641196034765 2304 60-61 0.44990552745564116 2304 62-63 0.3847952211319452 2304 64-65 0.3930969006703151 2304 66-67 0.4996658901498492 2304 68-69 0.24049709414785525 2304 70-71 0.45682829695076066 2304 72-73 0.25416906872776934 2304 74-75 0.43688765825719145 2304 76-77 0.5479645084452471 2304 78-79 0.6437108933570883 2304 80-81 0.5449689038774856 2304 82-83 0.6056071703617647 2304 84-85 0.610716409281558 2304 86-87 0.40844381318526146 2304 88-89 0.4677234267044206 2304 90-91 0.6559002062799344 2304 92-93 0.5961895114963909 2304 94-95 0.5228592723212238 2304 96-97 0.5419178426358364 2304 98-99 0.3789870890294047 2304 100-101 0.30411123419036556 2202 1 0.1675075430905011 2202 2 0.05914769746440385 2202 3 0.09008024908298395 2202 4 0.17962938184260224 2202 5 0.17739200179968861 2202 6 0.2623145101811133 2202 7 0.41951321943406583 2202 8 0.1462002666685649 2202 9 0.19476583398864733 2202 10-11 0.13525328381554402 2202 12-13 0.19199124447720095 2202 14-15 0.34263480226179155 2202 16-17 0.2673045406751129 2202 18-19 0.20981695351741791 2202 20-21 0.23697690852423392 2202 22-23 0.16802978803053747 2202 24-25 0.3260882051418008 2202 26-27 0.38462113633274697 2202 28-29 0.28915953846009046 2202 30-31 0.3747176437486601 2202 32-33 0.3478665480958867 2202 34-35 0.17333503057226807 2202 36-37 0.3397176870185632 2202 38-39 0.23612210276275647 2202 40-41 0.23308519937309313 2202 42-43 0.25957661506708263 2202 44-45 0.3579554759791037 2202 46-47 0.11870906996784925 2202 48-49 0.3447886312113795 2202 50-51 0.23764199941735598 2202 52-53 0.2409487356874891 2202 54-55 0.3576216353876802 2202 56-57 0.43985735864354325 2202 58-59 0.3683636498658558 2202 60-61 0.3154808685717825 2202 62-63 0.41009760088351044 2202 64-65 0.43666195396860275 2202 66-67 0.32102903946109507 2202 68-69 0.38183805040494434 2202 70-71 0.38651452608487347 2202 72-73 0.3286911589819752 2202 74-75 0.43545623315655035 2202 76-77 0.46736252857447624 2202 78-79 0.45229066746123436 2202 80-81 0.43338988934394607 2202 82-83 0.3462931894955119 2202 84-85 0.37085151848297215 2202 86-87 0.28460857571004894 2202 88-89 0.5734384192884008 2202 90-91 0.5010459340681201 2202 92-93 0.44777080938668234 2202 94-95 0.32495602455183814 2202 96-97 0.3851033062098175 2202 98-99 0.3893969711236096 2202 100-101 0.4599514080188385 1102 1 0.0016293320820217616 1102 2 -0.0908775166572795 1102 3 -0.14024021645301232 1102 4 -0.08732084354434022 1102 5 -0.09063455505273055 1102 6 0.022170501492801975 1102 7 0.07088422125727334 1102 8 0.03308824666154919 1102 9 -0.036561982963128514 1102 10-11 -0.005205802217467692 1102 12-13 -0.052411908909824945 1102 14-15 -0.021646996642694205 1102 16-17 -0.05358073285434983 1102 18-19 -0.008848917249530075 1102 20-21 -0.04026193863150951 1102 22-23 0.051887896348354445 1102 24-25 0.03452344311947542 1102 26-27 0.1981722157561805 1102 28-29 0.11505671664867378 1102 30-31 0.17208520076979994 1102 32-33 0.10559351544494433 1102 34-35 0.044722988046395074 1102 36-37 0.12121686898890971 1102 38-39 0.08009991806937222 1102 40-41 0.04811139242342932 1102 42-43 0.040252236850129464 1102 44-45 0.1293526510173777 1102 46-47 0.046675369619890716 1102 48-49 0.1339224065366338 1102 50-51 0.0324918779671961 1102 52-53 0.05982651708747255 1102 54-55 0.21378668065759143 1102 56-57 0.34496167230022223 1102 58-59 0.16011932047631916 1102 60-61 0.24384714050112422 1102 62-63 0.24744334378066668 1102 64-65 0.2033592213925388 1102 66-67 0.24705773712195622 1102 68-69 0.2802990205547431 1102 70-71 0.28361265020556203 1102 72-73 0.3850590373343934 1102 74-75 0.6108898378751988 1102 76-77 0.43908787223210766 1102 78-79 0.3697693159656481 1102 80-81 0.36817585633760075 1102 82-83 0.3360916562062215 1102 84-85 0.30604252158030576 1102 86-87 0.23694469150318653 1102 88-89 0.3548180529520657 1102 90-91 0.2868993533293711 1102 92-93 0.36932287450608925 1102 94-95 0.27146015250376365 1102 96-97 0.3930340283978033 1102 98-99 0.4081389859711244 1102 100-101 0.29114590865671985 2305 1 0.14400551171490505 2305 2 -0.007714588211346296 2305 3 0.009994883918384545 2305 4 -0.05546702875591336 2305 5 -0.02127972097814279 2305 6 0.27043026122505154 2305 7 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145613.0 11 99979.0 12 57982.0 13 37242.0 14 26179.0 15 20455.0 16 17772.0 17 16846.0 18 16861.0 19 17248.0 20 18330.0 21 19960.0 22 21613.0 23 22729.0 24 24788.0 25 28238.0 26 33121.0 27 40146.0 28 50451.0 29 63325.0 30 76197.0 31 98277.0 32 135038.0 33 194108.0 34 296751.0 35 508580.0 36 1037810.0 37 1739989.0 38 378496.0 39 70.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.232676878733365 23.978717436933206 22.607658548199407 14.180947136134023 2 30.77410118041212 26.649739212139302 29.777929835372667 12.798229772075906 3 34.64012449723023 20.23668889681105 28.992457826414643 16.130728779544082 4 27.57581432193002 17.68293657118887 33.730095477799566 21.01115362908155 5 33.92022650261157 16.66287316660142 31.088795214727277 18.328105116059735 6 31.008888541219758 17.031579897927728 35.705134625064545 16.25439693578797 7 35.25008215540105 19.47609654007335 27.872796502975707 17.40102480154989 8 27.29066817552181 31.45585883535677 24.00888966329583 17.24458332582559 9 32.04099602420135 26.94422162695542 23.172552484275194 17.842229864568033 10-11 26.723022682579906 29.796203020933277 26.646504455949465 16.834269840537353 12-13 28.89182542031712 23.99794967033214 26.336557026906814 20.773667882443927 14-15 25.502621198444974 24.343418742662458 28.46295434470132 21.69100571419125 16-17 27.19802236660499 25.334355324818038 29.478631099802705 17.988991208774266 18-19 26.84225433993033 25.554869367934526 29.730154912272717 17.872721379862426 20-21 25.095085706919917 28.46184642767956 28.499272421954963 17.943795443445563 22-23 28.68549445885168 25.801427346778944 27.498994113591728 18.014084080777646 24-25 24.532694614347335 29.711412383499592 27.41700626550545 18.33888673664762 26-27 22.694922813508168 31.623408555181804 27.05180930076675 18.62985933054328 28-29 24.011949573713466 29.929698713152142 26.952064059946018 19.10628765318837 30-31 26.69864083452318 26.786780160649094 27.537781498864867 18.976797505962857 32-33 22.716525069405424 29.156963421014876 29.485400755009756 18.641110754569944 34-35 27.410215110700843 26.849493359734534 27.929746974147164 17.810544555417458 36-37 23.777223910908756 27.20193226151169 29.759701982931404 19.26114184464815 38-39 20.531740172339703 29.707428335700204 31.138747492978204 18.622083998981896 40-41 21.843827843929216 30.664489846024505 28.33823365977893 19.15344865026735 42-43 24.87945979231555 27.216265697066948 29.69046236625263 18.213812144364873 44-45 22.246567943115085 29.3481702935229 30.07151720848244 18.33374455487957 46-47 23.95483828235962 30.216431603267207 27.454853724616555 18.37387638975661 48-49 21.12809391541207 29.279018451284067 29.635183645120485 19.957703988183383 50-51 19.945465701550486 27.09151090444107 31.341491276409933 21.62153211759851 52-53 25.555785403008365 26.612766405496295 27.590434534464038 20.241013657031303 54-55 27.271689981788317 26.687528641741654 27.26971853723278 18.771062839237253 56-57 27.800808452498977 26.241533114542182 26.91290237386756 19.044756059091288 58-59 25.792245988310015 26.04870394416189 27.430025634633914 20.72902443289418 60-61 21.65593860597386 26.65130712615608 26.617135898831595 25.075618369038473 62-63 24.457775212023346 26.821894809646118 27.035856418151038 21.68447356017949 64-65 21.925753887763257 28.32576980380687 29.821589704104756 19.926886604325112 66-67 20.5999237780571 28.15680224124868 28.346380847707387 22.896893132986836 68-69 20.06868931025515 26.570742392956898 33.710054940104435 19.650513356683522 70-71 19.661591068772218 31.049661334496953 30.498701244615013 18.790046352115816 72-73 19.561964767082923 35.457431819103036 26.47462955532508 18.505973858488964 74-75 20.430506879034766 35.09477701693712 25.808593719858813 18.666122384169302 76-77 20.927044179168806 34.03942728203847 26.249914120568125 18.783614418224605 78-79 20.55677661671343 33.766308263188044 27.031923293579556 18.644991826518968 80-81 19.81731640793682 34.14418487359326 26.768189786218244 19.270308932251673 82-83 19.13321385029788 33.634006368605135 27.22729823101432 20.005481550082667 84-85 19.279135326196783 32.783046745174346 27.651438393535276 20.286379535093594 86-87 19.281081796933737 32.48657909489662 28.257947731475507 19.974391376694143 88-89 19.763346119737886 32.854022956010404 27.743202696750924 19.639428227500787 90-91 20.347633270189547 31.74173184422467 27.56703661952189 20.343598266063896 92-93 20.006053415066834 32.35882212738869 26.95655819477346 20.678566262771014 94-95 19.64181127528908 32.723560003049286 26.8953072619466 20.73932145971504 96-97 20.367626813719376 32.90222242961568 26.663187661939645 20.066963094725292 98-99 20.849244953822907 31.74302044131492 26.686960277863985 20.72077432699819 100-101 20.828794084418266 31.650484389586914 26.26008387462569 21.26063765136913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2883.0 1 1833.5 2 620.0 3 390.5 4 331.0 5 347.5 6 400.5 7 575.5 8 834.5 9 1110.5 10 1357.5 11 1567.0 12 1734.5 13 1651.0 14 1484.5 15 1407.5 16 1264.5 17 1175.0 18 1100.0 19 1106.0 20 1266.0 21 1392.5 22 1571.0 23 1818.5 24 2188.5 25 2723.0 26 3378.0 27 4209.5 28 5245.0 29 6408.5 30 8203.5 31 11864.5 32 21222.5 33 39881.0 34 70856.5 35 113566.0 36 170892.5 37 250125.5 38 325105.0 39 352621.5 40 357052.5 41 339111.5 42 318915.0 43 344846.0 44 353636.5 45 339592.0 46 350952.0 47 329651.0 48 262170.5 49 211077.0 50 175998.0 51 146397.5 52 116322.5 53 85801.5 54 64725.5 55 49096.5 56 37225.0 57 27218.0 58 18605.0 59 13112.5 60 9631.0 61 7195.5 62 5283.0 63 3694.0 64 2680.0 65 1955.5 66 1475.5 67 1138.0 68 863.0 69 633.0 70 445.5 71 346.0 72 279.0 73 224.5 74 178.0 75 134.0 76 122.5 77 102.0 78 77.5 79 66.0 80 56.0 81 42.5 82 38.0 83 37.5 84 33.0 85 20.5 86 13.5 87 15.5 88 15.0 89 13.0 90 9.0 91 8.0 92 8.0 93 5.0 94 4.0 95 2.5 96 0.0 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2642816255590751 2 0.2525106476373516 3 0.21675282651765904 4 0.21877335658611238 5 0.23376976782811926 6 0.23636494406282996 7 0.21378691082084683 8 0.20933803727562847 9 0.19087521206297234 10-11 0.17855739343464902 12-13 0.20575113297979616 14-15 0.20247008874019765 16-17 0.20423110118517993 18-19 0.19665874767175617 20-21 0.19770608665219297 22-23 0.16362586159850992 24-25 0.47350844099607314 26-27 0.3319230404194991 28-29 0.275283319096938 30-31 0.27940779561281753 32-33 0.3214774560747885 34-35 0.3168061388523093 36-37 0.3091411004733602 38-39 0.3332113600503019 40-41 0.3388836738204553 42-43 0.3739370899621549 44-45 0.3404407795612817 46-47 0.366327662502521 48-49 0.342322282331447 50-51 0.3643998172995931 52-53 0.35364837289864876 54-55 0.33099619176424533 56-57 0.36680035531670047 58-59 0.3537595947372792 60-61 0.32055987590608725 62-63 0.29824136028757514 64-65 0.30406196984256917 66-67 0.3134138727740803 68-69 0.31925301930217936 70-71 0.2968325503315894 72-73 0.3032278060528408 74-75 0.30195802339514305 76-77 0.30483125422642987 78-79 0.30698154310661874 80-81 0.32361847646842484 82-83 0.34121933243169494 84-85 0.33593629509674816 86-87 0.33941197755395 88-89 0.3351484737397824 90-91 0.30931720171785837 92-93 0.2936627279306213 94-95 0.3024121792362174 96-97 0.29086364499175477 98-99 0.310327466752085 100-101 0.3319693828522618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5394624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.787352267617454 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12817715700625 27.377867074575207 2 5.290504727541544 3.3634227489571713 3 2.2307333862045162 2.1272732388725424 4 1.3331457705345604 1.695086969282655 5 0.7420285104983741 1.179356082791365 6 0.44518466063079326 0.8490744978966451 7 0.40521967953831317 0.9016602489478793 8 0.322253786197996 0.8194875697159343 9 0.25404341661017754 0.7267830817551131 >10 2.2234131959168115 14.202129296174077 >50 0.29272471576152315 6.50629948962281 >100 0.2674486334644963 18.176879766731098 >500 0.04290046807934941 9.33801132148976 >1k 0.021694773974403277 11.200253783082028 >5k 4.685516170150414E-4 1.0295933669584496 >10k+ 5.8566424020010416E-5 0.5068214631473437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 27224 0.5046505558126015 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9169 0.16996550640044608 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 8955 0.16599859415595972 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8272 0.15333784152519248 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8088 0.1499270384738584 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 5462 0.10124894709992764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005727924689468627 0.0 0.0 2 0.006117201124675232 0.0 0.0 3 0.006506477559881838 0.0 0.0 4 0.006877217021983367 0.0 0.0 5 0.007563085026871196 0.0 0.0 6 0.008341637897284407 0.0 0.0 7 0.009305560498748384 0.0 0.0 8 0.01032509401952759 0.0 0.0 9 0.011529997271357558 0.0 0.0 10-11 0.017461828664982026 0.0 0.0 12-13 0.022707792053718666 0.0 0.0 14-15 0.028417179770082214 0.0 0.0 16-17 0.03286605331530057 0.0 0.0 18-19 0.037852499080566135 0.0 0.0 20-21 0.043654571662455066 0.0 0.0 22-23 0.05014251224923183 0.0 0.0 24-25 0.05748315359884211 0.0 0.0 26-27 0.06479598948879477 0.0 0.0 28-29 0.07323958073815709 0.0 0.0 30-31 0.11446580892384715 0.0 1.853697310507646E-5 32-33 0.12598653770865215 0.0 1.853697310507646E-5 34-35 0.13664529724407115 0.0 1.853697310507646E-5 36-37 0.14833285878682184 0.0 1.853697310507646E-5 38-39 0.16009456822199286 0.0 1.853697310507646E-5 40-41 0.17257921960826186 9.26848655253823E-6 1.853697310507646E-5 42-43 0.1871122065226418 1.853697310507646E-5 1.853697310507646E-5 44-45 0.2027018009040111 1.853697310507646E-5 1.853697310507646E-5 46-47 0.21940361367168498 1.853697310507646E-5 1.853697310507646E-5 48-49 0.23714349693324316 1.853697310507646E-5 1.853697310507646E-5 50-51 0.2560419410138686 1.853697310507646E-5 1.853697310507646E-5 52-53 0.2762750471580596 1.853697310507646E-5 1.853697310507646E-5 54-55 0.2982506287741278 1.853697310507646E-5 1.853697310507646E-5 56-57 0.3204579225540093 1.853697310507646E-5 1.853697310507646E-5 58-59 0.3451306337568661 1.853697310507646E-5 1.853697310507646E-5 60-61 0.3717497271357559 1.853697310507646E-5 1.853697310507646E-5 62-63 0.40028739723102114 1.853697310507646E-5 3.707394621015292E-5 64-65 0.4313646326416818 1.853697310507646E-5 3.707394621015292E-5 66-67 0.4644067872014806 1.853697310507646E-5 3.707394621015292E-5 68-69 0.5002294877270409 1.853697310507646E-5 3.707394621015292E-5 70-71 0.5385176056755763 1.853697310507646E-5 3.707394621015292E-5 72-73 0.5793452889395072 1.853697310507646E-5 3.707394621015292E-5 74-75 0.6225827787070981 1.853697310507646E-5 3.707394621015292E-5 76-77 0.6698427916384905 1.853697310507646E-5 3.707394621015292E-5 78-79 0.7223024255258568 1.853697310507646E-5 3.707394621015292E-5 80-81 0.7778855393814286 1.853697310507646E-5 3.707394621015292E-5 82-83 0.8390204766819708 1.853697310507646E-5 3.707394621015292E-5 84-85 0.9051140542881209 1.853697310507646E-5 3.707394621015292E-5 86-87 0.9768336032316618 1.853697310507646E-5 3.707394621015292E-5 88-89 1.05522646249303 1.853697310507646E-5 3.707394621015292E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4975 0.0 91.04298 9 ATTCGGA 1335 0.0 89.9193 2 CGGGAGA 6340 0.0 89.27306 4 AGAGCGG 5330 0.0 87.7417 8 GAGAGCG 5950 0.0 86.81513 7 CGGAAAG 3110 0.0 85.422585 5 AAGCGGC 2080 0.0 85.27946 9 CGGAGAG 2200 0.0 83.88262 5 GTCCGGA 465 0.0 82.65054 2 TCGGGAG 7040 0.0 82.217545 3 GGATCGG 2685 0.0 80.59701 1 TTCGGAG 1215 0.0 80.43929 3 GTTCGGA 1095 0.0 79.72918 2 TCGGAGA 1685 0.0 79.11807 4 TTGGGAG 3790 0.0 76.61072 3 ACCGGAA 710 0.0 76.176994 3 TCGGGAA 2670 0.0 75.51878 3 AAGGGGC 4790 0.0 75.44936 9 AAAGCGG 2440 0.0 75.42203 8 CCGGAGA 1050 0.0 75.00463 4 >>END_MODULE