FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394624
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT272240.5046505558126015Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC91690.16996550640044608No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG89550.16599859415595972No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82720.15333784152519248No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG80880.1499270384738584No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA54620.10124894709992764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC49750.091.042989
ATTCGGA13350.089.91932
CGGGAGA63400.089.273064
AGAGCGG53300.087.74178
GAGAGCG59500.086.815137
CGGAAAG31100.085.4225855
AAGCGGC20800.085.279469
CGGAGAG22000.083.882625
GTCCGGA4650.082.650542
TCGGGAG70400.082.2175453
GGATCGG26850.080.597011
TTCGGAG12150.080.439293
GTTCGGA10950.079.729182
TCGGAGA16850.079.118074
TTGGGAG37900.076.610723
ACCGGAA7100.076.1769943
TCGGGAA26700.075.518783
AAGGGGC47900.075.449369
AAAGCGG24400.075.422038
CCGGAGA10500.075.004634
Produced by FastQC (version 0.11.2)