FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881122
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT117350.40730659791567314Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33600.11662123297798567No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32880.11412220655702883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC31650.087.535729
AAGCGGC12500.086.9103859
CGGGAGA40350.084.92074
GAGAGCG39650.084.00857
CGGAAAG21450.082.3024755
ATTCGGA8700.080.742132
ATCGGGA53350.080.069252
TCGGGAG44400.079.419253
GATCGGG54650.079.301911
ATCCGGA9600.078.116632
GTTCGGA7850.076.183092
AGAGCGG38900.075.007038
TCGGAGA11300.074.758654
GATCCGG10200.073.994591
TTCGGAA9900.073.825333
TCGGGAA15150.072.9899753
GGATCGG17850.072.332541
TTCGGAG8300.072.046413
AAAGCGG16100.071.6072168
AAGCGTC4700.070.655579
Produced by FastQC (version 0.11.2)