##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2881122 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.77772860711903 33.0 33.0 33.0 22.0 33.0 2 30.1688647686561 33.0 33.0 33.0 22.0 33.0 3 29.98726086573217 33.0 33.0 33.0 15.0 33.0 4 33.23493798596519 37.0 37.0 37.0 15.0 37.0 5 33.31922702336104 37.0 37.0 37.0 15.0 37.0 6 34.05229073951051 37.0 37.0 37.0 27.0 37.0 7 33.331644755064175 37.0 37.0 37.0 15.0 37.0 8 33.789827712953496 37.0 37.0 37.0 22.0 37.0 9 32.98060477827735 37.0 37.0 37.0 15.0 37.0 10-11 33.040576553162275 37.0 37.0 37.0 15.0 37.0 12-13 33.045793964990025 37.0 37.0 37.0 15.0 37.0 14-15 34.58157724664211 40.0 37.0 40.0 15.0 40.0 16-17 34.49778367594291 40.0 37.0 40.0 15.0 40.0 18-19 34.56341036582276 40.0 37.0 40.0 15.0 40.0 20-21 34.5431429144618 40.0 37.0 40.0 15.0 40.0 22-23 35.50488264641345 40.0 37.0 40.0 24.5 40.0 24-25 34.72767414916828 40.0 37.0 40.0 15.0 40.0 26-27 34.6222627851233 38.5 37.0 40.0 18.5 40.0 28-29 34.17230197124592 37.0 37.0 40.0 15.0 40.0 30-31 34.18020045662766 37.0 37.0 40.0 15.0 40.0 32-33 34.037512989731084 37.0 37.0 40.0 15.0 40.0 34-35 34.13807207747537 37.0 37.0 40.0 15.0 40.0 36-37 33.969141362288724 37.0 35.0 40.0 15.0 40.0 38-39 33.79823120992447 37.0 33.0 40.0 15.0 40.0 40-41 33.8547227434312 37.0 33.0 40.0 15.0 40.0 42-43 33.676757353558784 37.0 33.0 40.0 15.0 40.0 44-45 33.488093180365155 37.0 33.0 40.0 15.0 40.0 46-47 33.42837842340589 37.0 33.0 40.0 10.5 40.0 48-49 33.33185300726592 37.0 33.0 40.0 10.5 40.0 50-51 32.45324165377238 37.0 33.0 38.5 15.0 38.5 52-53 32.8693897724567 37.0 33.0 38.5 15.0 40.0 54-55 33.43958916005639 37.0 33.0 40.0 10.5 40.0 56-57 33.80109814856851 37.0 33.0 40.0 18.5 40.0 58-59 33.17890807817233 37.0 33.0 40.0 6.0 40.0 60-61 33.024177907079256 37.0 33.0 40.0 10.5 40.0 62-63 32.63051772885702 37.0 33.0 40.0 6.0 40.0 64-65 32.47169956704367 37.0 33.0 40.0 6.0 40.0 66-67 32.412458063212874 37.0 33.0 37.0 10.5 40.0 68-69 32.57672965601596 37.0 33.0 37.0 15.0 40.0 70-71 32.12232977291485 37.0 33.0 37.0 15.0 40.0 72-73 32.534957735215656 37.0 33.0 37.0 18.5 40.0 74-75 32.69118020687774 37.0 33.0 37.0 22.0 37.0 76-77 32.35368772998852 37.0 33.0 37.0 22.0 37.0 78-79 31.790175841217412 37.0 33.0 37.0 18.5 37.0 80-81 31.222311481429806 37.0 33.0 37.0 15.0 37.0 82-83 30.783961248430302 35.0 33.0 37.0 15.0 37.0 84-85 30.577029365642968 33.0 27.0 37.0 15.0 37.0 86-87 30.382137757443104 33.0 27.0 37.0 10.5 37.0 88-89 30.155481614454366 33.0 27.0 37.0 6.0 37.0 90-91 30.067544692657933 33.0 27.0 37.0 6.0 37.0 92-93 29.912502143262245 33.0 27.0 37.0 6.0 37.0 94-95 29.785034788530304 33.0 27.0 37.0 6.0 37.0 96-97 29.64501902383863 33.0 27.0 37.0 6.0 37.0 98-99 29.5073927796185 33.0 27.0 37.0 6.0 37.0 100-101 28.38277726524597 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.04395155175105003 2306 2 0.03924961026361018 2306 3 -0.005902304927136726 2306 4 0.22196840191001144 2306 5 0.1732183054445997 2306 6 -0.03295717911476714 2306 7 0.5247354319449897 2306 8 -0.15027029240047796 2306 9 0.27801593317491324 2306 10-11 0.2502426606898638 2306 12-13 0.2932426510168611 2306 14-15 0.7053235096826782 2306 16-17 0.49381630668259646 2306 18-19 0.5468855509106021 2306 20-21 0.4273304391306425 2306 22-23 0.0796132291760756 2306 24-25 0.541241904211283 2306 26-27 0.20215346198003914 2306 28-29 0.5157371543243485 2306 30-31 0.5890201632712007 2306 32-33 0.5480552012048818 2306 34-35 0.3855443110619845 2306 36-37 0.4215737465902123 2306 38-39 0.5895575286520014 2306 40-41 0.35678467668549274 2306 42-43 0.34105067771111663 2306 44-45 0.5175003323923804 2306 46-47 0.49796242430721804 2306 48-49 0.5040924773046029 2306 50-51 0.38007049470477483 2306 52-53 0.38399058332235114 2306 54-55 0.4879949710227365 2306 56-57 0.45791593866091773 2306 58-59 0.754499639551419 2306 60-61 0.47643922328852284 2306 62-63 0.48117769463753035 2306 64-65 0.5530050788536656 2306 66-67 0.494633052058262 2306 68-69 0.2521041836412934 2306 70-71 0.5158679964998996 2306 72-73 0.0865766743561096 2306 74-75 0.23119786083527316 2306 76-77 0.41066834055415313 2306 78-79 0.5098632524172828 2306 80-81 0.48357862164630916 2306 82-83 0.6174108297936094 2306 84-85 0.6222987867434 2306 86-87 0.6234869944359183 2306 88-89 0.6708040133405184 2306 90-91 0.6530380265972688 2306 92-93 0.6942942277313691 2306 94-95 0.5833710734847735 2306 96-97 0.6455638970569417 2306 98-99 0.7837590751979491 2306 100-101 0.5123776420307742 2307 1 -0.006328643689361257 2307 2 -0.01965826783197855 2307 3 -0.14299943342630783 2307 4 0.03891753860892777 2307 5 -0.00468799196545433 2307 6 -0.1324328701155153 2307 7 0.5682221382020174 2307 8 -0.17956317165049285 2307 9 0.16323928095658147 2307 10-11 0.07043140819053662 2307 12-13 -0.03538103084937205 2307 14-15 0.23961756206122686 2307 16-17 0.2462003749427879 2307 18-19 0.1669954978253685 2307 20-21 0.09044989539724924 2307 22-23 -0.09051779666172877 2307 24-25 0.21489016569088903 2307 26-27 -0.003205351767341824 2307 28-29 0.13227265805452504 2307 30-31 0.2787898281303427 2307 32-33 0.45419005583509175 2307 34-35 0.39311159769514603 2307 36-37 0.2453762908302437 2307 38-39 0.21508162732941116 2307 40-41 0.32794749477391605 2307 42-43 0.18731624970709504 2307 44-45 0.3630685706150487 2307 46-47 0.17005935183217247 2307 48-49 0.2532930205893038 2307 50-51 0.25018450784194357 2307 52-53 0.11155826255629364 2307 54-55 0.15226643016093533 2307 56-57 0.21108530196526942 2307 58-59 0.25487485372917007 2307 60-61 0.2373879711257132 2307 62-63 0.3031523083230283 2307 64-65 0.24895465395678684 2307 66-67 0.3225123815335138 2307 68-69 0.07320423234248352 2307 70-71 0.1796114075837707 2307 72-73 -0.22074476940522914 2307 74-75 -0.027567746214337774 2307 76-77 0.08641122069219875 2307 78-79 0.3181201142844401 2307 80-81 0.30571242560204226 2307 82-83 0.2829359941017131 2307 84-85 0.23607346567541398 2307 86-87 0.4117666458438123 2307 88-89 0.5818583427456616 2307 90-91 0.4109786111424434 2307 92-93 0.48900664298334817 2307 94-95 0.38024428867019466 2307 96-97 0.36057844665504035 2307 98-99 0.3656647594029643 2307 100-101 0.26176270768391063 2201 1 0.04722516452660841 2201 2 -0.016978375986340666 2201 3 -0.056430671431595414 2201 4 0.13311817809024262 2201 5 0.17984675075960155 2201 6 0.00425315379135327 2201 7 0.5082071187352994 2201 8 -0.0320986387186295 2201 9 0.1357822400045663 2201 10-11 0.05626339965465377 2201 12-13 0.10049099477249257 2201 14-15 0.2929963207005315 2201 16-17 0.21582021910250404 2201 18-19 0.19766547966353443 2201 20-21 0.09900593167831317 2201 22-23 -0.08496529865456637 2201 24-25 0.26504515380491256 2201 26-27 0.18804714038117254 2201 28-29 0.2919416616744641 2201 30-31 0.3011525890847011 2201 32-33 0.4563460416962002 2201 34-35 0.2705761838875347 2201 36-37 0.18179539076296436 2201 38-39 0.2428369271950146 2201 40-41 0.3049144501717791 2201 42-43 0.2920786278958474 2201 44-45 0.3455847396213372 2201 46-47 0.19253103055051213 2201 48-49 0.16930251161401344 2201 50-51 0.20538859327892567 2201 52-53 0.293728504483326 2201 54-55 0.4803724505526148 2201 56-57 0.3223943745398259 2201 58-59 0.4755879588352556 2201 60-61 0.3159115539964219 2201 62-63 0.4543653166725079 2201 64-65 0.3471103309068795 2201 66-67 0.3157301868805078 2201 68-69 0.20852092827285418 2201 70-71 0.5036312808592385 2201 72-73 0.12006814591588011 2201 74-75 0.3202613549788751 2201 76-77 0.3647896686451375 2201 78-79 0.35900812693913053 2201 80-81 0.5893749385399083 2201 82-83 0.4727263074333514 2201 84-85 0.5016993921865485 2201 86-87 0.4962877183686665 2201 88-89 0.4788260437908143 2201 90-91 0.44034646202718974 2201 92-93 0.32115904263608996 2201 94-95 0.3345015795915849 2201 96-97 0.47120596210502796 2201 98-99 0.44172202511437675 2201 100-101 0.2629940909128834 1101 1 -0.2039618453511416 1101 2 -0.31262877288458313 1101 3 -0.2649466834299865 1101 4 -0.11637836745324392 1101 5 -0.1649276844028691 1101 6 -0.1307750724499357 1101 7 0.245399328637518 1101 8 -0.26071682568760224 1101 9 -0.021247715070764173 1101 10-11 -0.1232008308232011 1101 12-13 -0.11018622880253304 1101 14-15 0.08858552890873739 1101 16-17 -0.02521538426361758 1101 18-19 -0.02246125529782006 1101 20-21 -0.07721335438827737 1101 22-23 -0.10736162692749218 1101 24-25 0.024883179567041225 1101 26-27 -0.2119698639895944 1101 28-29 -0.11141857777953845 1101 30-31 0.01286047022178849 1101 32-33 -0.12469973048647631 1101 34-35 -0.12146161246771925 1101 36-37 -0.07330237084658364 1101 38-39 -0.09545928218254573 1101 40-41 -0.19945023036409282 1101 42-43 -0.1465553700954061 1101 44-45 0.08431461116227723 1101 46-47 -0.06261380578326481 1101 48-49 0.08379900813893926 1101 50-51 -0.07407612738456493 1101 52-53 -0.0067320502783516645 1101 54-55 -0.002137641355503206 1101 56-57 0.10045141727353268 1101 58-59 0.02504202725773297 1101 60-61 -4.733573459034801E-4 1101 62-63 0.07174652187334374 1101 64-65 -0.041542226526821935 1101 66-67 -0.006809427725393391 1101 68-69 -0.33811043150549125 1101 70-71 0.01650904918263052 1101 72-73 -0.2111846874416301 1101 74-75 -0.020962699410773666 1101 76-77 -0.05408942554590368 1101 78-79 -0.0713198444394294 1101 80-81 0.19259383550052078 1101 82-83 0.1147621599490023 1101 84-85 0.010588570679367137 1101 86-87 0.0630606369981841 1101 88-89 0.06397213432370208 1101 90-91 0.05936708464408369 1101 92-93 0.03565455878973367 1101 94-95 0.1932441925785291 1101 96-97 -0.00714583432391791 1101 98-99 -0.04856858059618574 1101 100-101 0.03207575009323094 2304 1 0.2739731186562686 2304 2 0.08682266673248407 2304 3 0.18992882944927203 2304 4 0.35159183800363536 2304 5 0.34155736838065565 2304 6 0.20316043143451878 2304 7 0.7382240134772147 2304 8 0.23819626275989236 2304 9 0.4058650297242252 2304 10-11 0.40466914186676206 2304 12-13 0.43808258592037674 2304 14-15 0.5635293035144784 2304 16-17 0.5528667717460607 2304 18-19 0.5111396412252702 2304 20-21 0.43395661045207135 2304 22-23 0.3769472525869588 2304 24-25 0.5355122448671494 2304 26-27 0.5317136768235784 2304 28-29 0.5023141041147525 2304 30-31 0.6284728900995162 2304 32-33 0.660849843738994 2304 34-35 0.5646390547806632 2304 36-37 0.5784599454524653 2304 38-39 0.5358005268815731 2304 40-41 0.6255216156457308 2304 42-43 0.5179432938190232 2304 44-45 0.5987748843444649 2304 46-47 0.5355580056819775 2304 48-49 0.6006670983380502 2304 50-51 0.4604487894669731 2304 52-53 0.5111328146587866 2304 54-55 0.6227838821989096 2304 56-57 0.693850696776785 2304 58-59 0.6872047633772524 2304 60-61 0.6656309373980136 2304 62-63 0.7231814040025455 2304 64-65 0.7094398793491692 2304 66-67 0.7149032252338685 2304 68-69 0.6585835294695173 2304 70-71 0.6714974139828342 2304 72-73 0.3379894280967761 2304 74-75 0.6903851637253595 2304 76-77 0.7924917385655519 2304 78-79 0.7362211461814638 2304 80-81 0.556110653095228 2304 82-83 0.6940462974584527 2304 84-85 0.8843731197821434 2304 86-87 0.6524773007940006 2304 88-89 0.7782521967996878 2304 90-91 0.8954658720758353 2304 92-93 0.8271653540497041 2304 94-95 0.7666594481635904 2304 96-97 0.8611945612996159 2304 98-99 0.5992899307180259 2304 100-101 0.64884602690449 2202 1 0.1019739744209538 2202 2 -0.0645881319297601 2202 3 -0.0767184771343068 2202 4 0.03628284004967952 2202 5 0.07568892529157978 2202 6 0.039541899570885164 2202 7 0.2746620179574819 2202 8 0.04479079718787915 2202 9 0.09820365669242648 2202 10-11 0.12352471099071494 2202 12-13 0.04976318572025917 2202 14-15 0.21942764690402328 2202 16-17 0.21254606584312086 2202 18-19 0.15547111917646816 2202 20-21 0.08857542389463902 2202 22-23 0.011041902807846782 2202 24-25 0.13801984124911826 2202 26-27 0.1952244293892207 2202 28-29 0.19528896712990473 2202 30-31 0.1799979377584009 2202 32-33 0.3221937618914197 2202 34-35 0.153854508244045 2202 36-37 0.29079935172527627 2202 38-39 0.1409232783855927 2202 40-41 0.13870112699233061 2202 42-43 0.20731277022189687 2202 44-45 0.21299109138070804 2202 46-47 0.2331428230637087 2202 48-49 0.18239446376647805 2202 50-51 0.30495039108498645 2202 52-53 0.12953587247363174 2202 54-55 0.2006175183092367 2202 56-57 0.2964920982899599 2202 58-59 0.23491636479676714 2202 60-61 0.15606673776958502 2202 62-63 0.25826371253806 2202 64-65 0.28654765938235727 2202 66-67 0.29034858679077047 2202 68-69 0.33634151421720304 2202 70-71 0.2973867218500601 2202 72-73 0.22978261535705258 2202 74-75 0.3620144323479835 2202 76-77 0.4283357805980117 2202 78-79 0.3559926922997505 2202 80-81 0.33488871817010235 2202 82-83 0.32403680611645314 2202 84-85 0.319516375819731 2202 86-87 0.38362448864390686 2202 88-89 0.546459435951359 2202 90-91 0.5051266025489234 2202 92-93 0.3912618349012327 2202 94-95 0.29944295513726615 2202 96-97 0.3522054296581594 2202 98-99 0.3457038429227488 2202 100-101 0.3879470475729718 1102 1 -0.09255748892024229 1102 2 -0.21966770929099866 1102 3 -0.215677424598546 1102 4 -0.2003164443492409 1102 5 -0.12544189246820991 1102 6 0.030101846916089414 1102 7 0.09855833282195903 1102 8 -0.004645194325732405 1102 9 -0.08971864947560704 1102 10-11 -0.041273247235544375 1102 12-13 -0.041346065979467994 1102 14-15 -0.10167033162795036 1102 16-17 -0.11593268668633527 1102 18-19 -0.07786244755578764 1102 20-21 -0.14751971884047066 1102 22-23 0.05744324158403913 1102 24-25 -0.053107615566247546 1102 26-27 0.022131590301441406 1102 28-29 -0.1537433609542873 1102 30-31 -0.04970370840416649 1102 32-33 -0.06863821189320873 1102 34-35 -0.12453566939586125 1102 36-37 -0.01306083298662486 1102 38-39 -0.04124060966694998 1102 40-41 -0.10589895563088447 1102 42-43 -0.0965911767872143 1102 44-45 -0.09986662163665727 1102 46-47 -0.01395311089373763 1102 48-49 -0.024822464190279447 1102 50-51 -0.22143407294939976 1102 52-53 -0.1738782869051576 1102 54-55 -0.04305375331808392 1102 56-57 0.177292353161576 1102 58-59 0.031105193857733582 1102 60-61 0.04217518424304245 1102 62-63 0.045762399669150966 1102 64-65 0.07060627985470802 1102 66-67 0.10785805695189055 1102 68-69 0.11418763897825812 1102 70-71 0.1529424330048954 1102 72-73 0.2705053584478634 1102 74-75 0.519433016970261 1102 76-77 0.32429389482810933 1102 78-79 0.2455121472665951 1102 80-81 0.2237090266767936 1102 82-83 0.22632539921111317 1102 84-85 0.12867214859187825 1102 86-87 0.15532953776271086 1102 88-89 0.39582540778944875 1102 90-91 0.24055830885840024 1102 92-93 0.24060224955774956 1102 94-95 0.34499145426552147 1102 96-97 0.33897206108158784 1102 98-99 0.32792724769948833 1102 100-101 0.24168819883647785 2305 1 0.2691825013244866 2305 2 0.22799049573932706 2305 3 0.2744729338015901 2305 4 0.25355570859642995 2305 5 0.21622238839873376 2305 6 0.5104097211155221 2305 7 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-0.16788110334208284 1213 74-75 -0.5935137793731684 1213 76-77 -0.5305816507952983 1213 78-79 -0.7068720455805888 1213 80-81 -1.1248980791637457 1213 82-83 -1.3831308866571845 1213 84-85 -1.2410039029669306 1213 86-87 -1.1462332034973777 1213 88-89 -1.1579618497022715 1213 90-91 -1.1633423957712985 1213 92-93 -1.205640588340433 1213 94-95 -0.984423067892731 1213 96-97 -1.1073818200217396 1213 98-99 -1.2392313314282717 1213 100-101 -1.1658528922346711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2196.0 3 362.0 4 417.0 5 299.0 6 532.0 7 4068.0 8 21081.0 9 66589.0 10 88945.0 11 62181.0 12 34676.0 13 22023.0 14 15405.0 15 12170.0 16 10371.0 17 9816.0 18 9704.0 19 9943.0 20 10443.0 21 11389.0 22 12108.0 23 12789.0 24 13517.0 25 15522.0 26 17939.0 27 21689.0 28 26569.0 29 33074.0 30 39946.0 31 50272.0 32 68915.0 33 98298.0 34 149338.0 35 251659.0 36 498386.0 37 884124.0 38 293948.0 39 419.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.94993840607448 22.079647557470263 23.600773925934174 13.369640110521077 2 30.776746390240273 26.612883583726454 31.102980013319346 11.507390012713925 3 36.23216937231113 19.753019002524834 29.624015535093612 14.39079609007043 4 27.16965972055555 17.562356738632133 35.646697901557914 19.621285639254396 5 35.03937076353365 16.208857247716637 31.864732249238585 16.88703973951113 6 31.185059247917945 16.402320168018406 37.59596193633071 14.81665864773294 7 36.00603523055402 19.144605543146405 28.737429211601057 16.11193001469853 8 27.113045274660152 33.055624823280354 24.372164877820428 15.459165024239066 9 33.55986519360685 27.46972350224005 22.599230552035795 16.371180752117304 10-11 26.934578600909752 30.72209943157026 26.729966145939272 15.613355821580722 12-13 29.438720911913325 23.82455629049709 26.701447226974285 20.03527557061531 14-15 25.50404587791682 24.264382276559466 29.14810947475315 21.083462370770565 16-17 27.326313446552263 25.244135386263316 30.229698277023825 17.199852890160592 18-19 27.274938648162646 25.389906270875883 30.68962003764744 16.645535043314034 20-21 25.20902996222186 28.932699194204208 29.181046976383573 16.67722386719036 22-23 29.369023081867507 25.91371302171636 28.012589224661035 16.704674671755104 24-25 24.221204128006487 30.96592429709916 27.966614553121556 16.846257021772793 26-27 22.42710478428281 33.00693069513786 27.792961500239215 16.773003020340113 28-29 24.34047245070097 30.83829667715544 27.695858773624536 17.125372098519055 30-31 27.69766369371329 27.07040243637155 28.06687839895584 17.16505547095932 32-33 27.171111161406802 26.821761165498163 29.045755343578566 16.961372329516472 34-35 22.697154584068443 26.780356563996875 28.342774413714654 22.179714438220028 36-37 19.221196832229058 27.04688204208526 29.452721664498497 24.279199461187183 38-39 19.280560482279984 29.092047541255923 31.448145881964546 20.179246094499547 40-41 21.492455705019374 26.81003003787384 33.84039005702842 17.85712420007836 42-43 23.148817196808697 26.433456433125457 32.90354666759573 17.514179702470127 44-45 21.032781866693043 29.051726509788377 32.485991600358446 17.429500023160134 46-47 23.772382256428603 30.536244018784558 28.24901643396151 17.442357290825328 48-49 20.106942894597896 29.378194599429097 31.088110691781846 19.426751814191164 50-51 19.229152706869606 27.146188707600466 32.69740815012361 20.927250435406325 52-53 25.759016521349015 26.568695680039234 28.06203308170948 19.61025471690227 54-55 27.490208894901 26.774826608682968 27.897245153113502 17.83771934330253 56-57 28.296673771617716 26.546162934520705 27.403947733060768 17.753215560800804 58-59 25.811903352875138 26.518832340220396 27.86769415860107 19.801570148303394 60-61 21.006208083078075 26.73962298839688 27.457649132055735 24.79651979646931 62-63 24.32580667171678 26.784567964842314 27.7717591096951 21.11786625374581 64-65 20.808771819884555 28.811786741308605 30.839982871347104 19.539458567459736 66-67 19.222381513194602 28.83553076535395 29.05973933729733 22.882348384154117 68-69 18.811655203367504 27.281636424982736 35.07346481568596 18.8332435559638 70-71 18.298062842689973 32.359907128961005 31.25572763630534 18.08630239204368 72-73 18.199230148328656 36.27438163900457 27.15555769857764 18.370830514089143 74-75 18.64528095895905 36.49434210819482 26.57999378926964 18.28038314357649 76-77 18.895868274991102 36.037491981732714 26.789777351103933 18.276862392172255 78-79 19.18211792738498 35.56761177383368 26.98949216207517 18.260778136706165 80-81 18.67221997624487 35.679230559499075 26.90886311412246 18.739686350133592 82-83 18.5268687013625 34.86273055932223 27.14301909009682 19.46738164921845 84-85 18.72624794029103 33.84800736489066 27.726107419514417 19.699637275303882 86-87 18.9063473790469 33.45282994883418 28.011453147221143 19.62936952489778 88-89 19.36640165598736 33.716608924299486 27.448307420513775 19.468681999199376 90-91 19.840542434252043 33.0521239974062 27.10822137793271 19.999112190409047 92-93 19.655924930848016 33.03464944312898 27.37261101043006 19.936814615592947 94-95 19.213824813078816 33.0294469730109 27.52814240214761 20.22858581176267 96-97 19.551836885014502 33.642779045040484 26.998427650515826 19.806956419429188 98-99 20.02285692401161 32.86200719653647 26.925909098030615 20.189226781421308 100-101 20.219833756967855 32.63666087542777 26.442420703034813 20.701084664569564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1580.0 1 1023.5 2 387.0 3 316.0 4 323.5 5 351.0 6 425.5 7 533.0 8 663.5 9 824.5 10 981.0 11 1126.5 12 1199.0 13 1139.0 14 1059.5 15 1024.5 16 995.5 17 961.5 18 977.5 19 1071.5 20 1303.0 21 1593.5 22 2022.0 23 2730.0 24 3817.0 25 5508.0 26 8393.5 27 12489.5 28 16991.0 29 21431.0 30 26070.5 31 30777.0 32 37403.0 33 48694.5 34 65083.5 35 88301.5 36 113658.0 37 141527.0 38 173611.5 39 189176.0 40 184692.0 41 170173.0 42 156379.5 43 154120.0 44 148219.5 45 140492.5 46 142794.5 47 135319.0 48 112378.5 49 92885.0 50 80206.5 51 69275.5 52 59665.0 53 50770.5 54 42177.5 55 33731.0 56 25901.0 57 19526.0 58 14447.0 59 10728.5 60 7990.5 61 5943.5 62 4391.0 63 3173.0 64 2275.5 65 1625.5 66 1173.0 67 864.5 68 660.5 69 476.0 70 359.0 71 296.5 72 220.5 73 153.5 74 122.0 75 114.0 76 105.5 77 86.0 78 67.0 79 52.0 80 44.0 81 36.0 82 28.0 83 24.5 84 27.5 85 29.0 86 28.0 87 24.5 88 19.5 89 17.5 90 15.5 91 11.5 92 8.5 93 6.0 94 2.5 95 2.5 96 4.5 97 4.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2589963215719432 2 0.24670944166890538 3 0.21113302387056154 4 0.21283375018482384 5 0.22654368679979534 6 0.2316805744428733 7 0.20839103654756724 8 0.20148400518964488 9 0.18374785934090956 10-11 0.17191219254165563 12-13 0.1999568223768379 14-15 0.19660743279874995 16-17 0.1985164113147586 18-19 0.19072430809941404 20-21 0.19150525385596306 22-23 0.1574039558199896 24-25 0.46320495973443676 26-27 0.3214025646952819 28-29 0.27388635399681094 30-31 0.2775654762276641 32-33 0.31991009058276604 34-35 0.3158144639484201 36-37 0.308265321635113 38-39 0.3330299792927894 40-41 0.3383057017370316 42-43 0.3756175545499288 44-45 0.34045764115507776 46-47 0.36695773382730756 48-49 0.34257487187283286 50-51 0.3653958423142095 52-53 0.35298748196015306 54-55 0.3300970941181942 56-57 0.3675130730319646 58-59 0.3534386950639369 60-61 0.32004892538393026 62-63 0.2977312310967741 64-65 0.3034234579445091 66-67 0.31305512227528026 68-69 0.31942416877869106 70-71 0.2962908200346948 72-73 0.3037184818969832 74-75 0.3010632663247166 76-77 0.3045514907039688 78-79 0.306738138822306 80-81 0.323519795413037 82-83 0.34071795640726077 84-85 0.3353033991618543 86-87 0.3391560648941627 88-89 0.3332902945449724 90-91 0.3083694477359862 92-93 0.2937744392635925 94-95 0.30271192958854226 96-97 0.28985235613070187 98-99 0.3091503934925352 100-101 0.33122512687765393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2881122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.72331035123159 #Duplication Level Percentage of deduplicated Percentage of total 1 92.36925070129644 57.013359279468354 2 3.8498134658227134 4.752464626906517 3 1.1569898114963733 2.142397236246107 4 0.49793861672447776 1.2293767910379159 5 0.3387294736069798 1.0453752212276461 6 0.2512866636040617 0.930614683485542 7 0.1806580680054438 0.7805569799267736 8 0.12995795551016945 0.6417148176452587 9 0.13672573230242693 0.7595248327111896 >10 0.913612833285533 11.107928186861837 >50 0.08744675981566202 3.8574203963268703 >100 0.07708355052430371 9.902481888059453 >500 0.008329854271882323 3.4133332987870073 >1k 0.002120424673675523 2.017185774164802 >5k 0.0 0.0 >10k+ 5.608905982534849E-5 0.4062659871447454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 11735 0.40730659791567314 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3360 0.11662123297798567 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3288 0.11412220655702883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005032761542204738 0.0 0.0 2 0.0055186833462796785 0.0 0.0 3 0.006178148651809955 0.0 0.0 4 0.0065252356547206266 0.0 0.0 5 0.007427661862288372 0.0 0.0 6 0.008260670669273985 0.0 0.0 7 0.009475475179461335 0.0 0.0 8 0.010690279689648686 0.0 0.0 9 0.011905084199836036 0.0 3.470870029106716E-5 10-11 0.018482382904993263 0.0 3.470870029106716E-5 12-13 0.02452169675563895 0.0 3.470870029106716E-5 14-15 0.0304915932057025 0.0 3.470870029106716E-5 16-17 0.03356331318146195 1.735435014553358E-5 3.470870029106716E-5 18-19 0.03833575947148368 3.470870029106716E-5 3.470870029106716E-5 20-21 0.04354206451514375 3.470870029106716E-5 3.470870029106716E-5 22-23 0.04966815011651711 3.470870029106716E-5 3.470870029106716E-5 24-25 0.055290959563669986 3.470870029106716E-5 3.470870029106716E-5 26-27 0.06169471476737188 3.470870029106716E-5 3.470870029106716E-5 28-29 0.06964300713402626 3.470870029106716E-5 3.470870029106716E-5 30-31 0.1373249727016072 3.470870029106716E-5 3.470870029106716E-5 32-33 0.1483623393941666 3.470870029106716E-5 3.470870029106716E-5 34-35 0.15697009706635123 3.470870029106716E-5 3.470870029106716E-5 36-37 0.16569933518955463 3.470870029106716E-5 3.470870029106716E-5 38-39 0.17526158211974363 3.470870029106716E-5 3.470870029106716E-5 40-41 0.18638572056303065 3.470870029106716E-5 3.470870029106716E-5 42-43 0.19884614396752376 3.470870029106716E-5 3.470870029106716E-5 44-45 0.21224370227987568 3.470870029106716E-5 3.470870029106716E-5 46-47 0.22638749764848554 3.470870029106716E-5 3.470870029106716E-5 48-49 0.24096515177073377 3.470870029106716E-5 3.470870029106716E-5 50-51 0.2574170757086996 3.470870029106716E-5 3.470870029106716E-5 52-53 0.27532676505889025 3.470870029106716E-5 3.470870029106716E-5 54-55 0.29389591971461115 3.470870029106716E-5 3.470870029106716E-5 56-57 0.3147211398892515 3.470870029106716E-5 3.470870029106716E-5 58-59 0.33788919733353884 3.470870029106716E-5 3.470870029106716E-5 60-61 0.36287946154310713 3.470870029106716E-5 3.470870029106716E-5 62-63 0.3873838039486006 3.470870029106716E-5 3.470870029106716E-5 64-65 0.4141789205733044 3.470870029106716E-5 6.941740058213432E-5 66-67 0.4432995201175098 3.470870029106716E-5 6.941740058213432E-5 68-69 0.4745199960293247 3.470870029106716E-5 6.941740058213432E-5 70-71 0.5111723835366916 3.470870029106716E-5 6.941740058213432E-5 72-73 0.5510006171206911 3.470870029106716E-5 6.941740058213432E-5 74-75 0.5949591860393277 3.470870029106716E-5 6.941740058213432E-5 76-77 0.6407399617232453 3.470870029106716E-5 6.941740058213432E-5 78-79 0.6890891812287019 3.470870029106716E-5 6.941740058213432E-5 80-81 0.7439115733384425 3.470870029106716E-5 6.941740058213432E-5 82-83 0.8031593247352941 3.470870029106716E-5 6.941740058213432E-5 84-85 0.865634985259215 3.470870029106716E-5 6.941740058213432E-5 86-87 0.939529808178897 3.470870029106716E-5 6.941740058213432E-5 88-89 1.016548414124775 3.470870029106716E-5 6.941740058213432E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3165 0.0 87.53572 9 AAGCGGC 1250 0.0 86.910385 9 CGGGAGA 4035 0.0 84.9207 4 GAGAGCG 3965 0.0 84.0085 7 CGGAAAG 2145 0.0 82.302475 5 ATTCGGA 870 0.0 80.74213 2 ATCGGGA 5335 0.0 80.06925 2 TCGGGAG 4440 0.0 79.41925 3 GATCGGG 5465 0.0 79.30191 1 ATCCGGA 960 0.0 78.11663 2 GTTCGGA 785 0.0 76.18309 2 AGAGCGG 3890 0.0 75.00703 8 TCGGAGA 1130 0.0 74.75865 4 GATCCGG 1020 0.0 73.99459 1 TTCGGAA 990 0.0 73.82533 3 TCGGGAA 1515 0.0 72.989975 3 GGATCGG 1785 0.0 72.33254 1 TTCGGAG 830 0.0 72.04641 3 AAAGCGG 1610 0.0 71.607216 8 AAGCGTC 470 0.0 70.65557 9 >>END_MODULE