FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906452
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT313490.6389342033713975Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC161300.089.805489
ATCGGGA257250.087.465842
GAGAGCG196000.086.447057
TCGGGAG229850.085.888843
AGAGCGG179750.083.3889548
GATCGGG277450.082.082691
GAGCGTC318650.081.585719
GGATCGG71050.080.8177641
CGGGAGA236600.080.310314
ATTGGGA111550.078.735242
GGAGAGC218800.078.533266
TCCGGAG36800.078.386143
AGAGCGT338250.077.703528
CGGAGAG95450.077.340745
TCCGGAA25450.076.619193
CGGAAGA337650.076.495324
AAGAGCG325200.076.4612357
ATCCGGA47600.075.26142
TCGGAAG352150.075.2317663
GAAGAGC335050.074.591386
Produced by FastQC (version 0.11.2)