##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_30749-1.3420000003587d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4906452 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.657027114501478 33.0 27.0 33.0 15.0 33.0 2 26.95997494727351 33.0 22.0 33.0 6.0 33.0 3 26.46545507833359 33.0 22.0 33.0 6.0 33.0 4 28.89370221088477 37.0 15.0 37.0 6.0 37.0 5 29.12696221220548 37.0 15.0 37.0 6.0 37.0 6 31.23762833102209 37.0 27.0 37.0 15.0 37.0 7 29.221836471649983 37.0 22.0 37.0 6.0 37.0 8 30.404334333648837 37.0 27.0 37.0 15.0 37.0 9 28.524703390555946 37.0 15.0 37.0 6.0 37.0 10-11 28.741626128208324 37.0 15.0 37.0 6.0 37.0 12-13 28.773027026454145 37.0 15.0 37.0 6.0 37.0 14-15 29.848086254588857 37.0 15.0 40.0 6.0 40.0 16-17 29.644280938649764 37.0 15.0 40.0 6.0 40.0 18-19 29.81165453162489 37.0 15.0 40.0 6.0 40.0 20-21 29.816116411614747 37.0 15.0 40.0 6.0 40.0 22-23 32.60004958776729 37.0 27.0 40.0 18.5 40.0 24-25 30.684927519926823 37.0 22.0 40.0 6.0 40.0 26-27 30.48050913368764 37.0 21.0 40.0 10.5 40.0 28-29 29.43156205339418 37.0 15.0 40.0 6.0 40.0 30-31 29.41630408286884 37.0 15.0 40.0 6.0 40.0 32-33 29.212116005618725 37.0 15.0 40.0 6.0 40.0 34-35 29.40741425779769 37.0 15.0 40.0 6.0 40.0 36-37 28.964669174385072 37.0 15.0 40.0 6.0 40.0 38-39 28.987071003649888 37.0 15.0 40.0 6.0 40.0 40-41 29.357866845533188 37.0 15.0 40.0 6.0 40.0 42-43 28.404036562469173 37.0 10.5 40.0 6.0 40.0 44-45 28.121910190907812 37.0 6.0 40.0 6.0 40.0 46-47 28.19250611235981 37.0 10.5 40.0 6.0 40.0 48-49 28.156417610933524 37.0 10.5 40.0 6.0 40.0 50-51 27.7121285401345 35.0 15.0 38.5 6.0 38.5 52-53 28.14692215474644 37.0 15.0 37.0 6.0 40.0 54-55 28.453524359353764 37.0 15.0 40.0 6.0 40.0 56-57 29.118453416032605 37.0 18.5 40.0 6.0 40.0 58-59 27.95872271857546 37.0 10.5 40.0 6.0 40.0 60-61 27.705846098158098 37.0 10.5 38.5 6.0 40.0 62-63 27.290336173674987 37.0 6.0 37.0 6.0 40.0 64-65 27.12723491435359 37.0 6.0 37.0 6.0 40.0 66-67 27.32057268673983 37.0 10.5 37.0 6.0 40.0 68-69 27.961759740032107 33.0 15.0 37.0 6.0 40.0 70-71 27.392238831644537 33.0 15.0 37.0 6.0 38.5 72-73 28.475386796813666 33.0 18.5 37.0 6.0 37.0 74-75 29.31462347945114 33.0 22.0 37.0 15.0 37.0 76-77 29.047825903524583 33.0 22.0 37.0 15.0 37.0 78-79 28.3318737246385 33.0 22.0 37.0 6.0 37.0 80-81 27.540123290720054 33.0 18.5 37.0 6.0 37.0 82-83 26.855478867417844 33.0 15.0 37.0 6.0 37.0 84-85 26.526567874301023 33.0 15.0 37.0 6.0 37.0 86-87 26.247210713566545 33.0 15.0 37.0 6.0 37.0 88-89 25.974220169686774 33.0 15.0 37.0 6.0 37.0 90-91 25.8451856861129 33.0 15.0 37.0 6.0 37.0 92-93 25.685774465948104 33.0 15.0 37.0 6.0 37.0 94-95 25.550861090661847 33.0 15.0 37.0 6.0 37.0 96-97 25.41787487169955 33.0 15.0 37.0 6.0 37.0 98-99 25.29509001616647 33.0 15.0 37.0 6.0 37.0 100-101 24.32592686120235 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.7921489239507586 2306 2 -0.8238919952916177 2306 3 -1.010086292655668 2306 4 -0.7101421446944904 2306 5 -0.7736208923692871 2306 6 -1.6042965312218591 2306 7 0.4732909804506704 2306 8 -1.6285740889405957 2306 9 -0.6988818687283462 2306 10-11 -1.0047879982868402 2306 12-13 -0.9203866508491672 2306 14-15 -0.10795248026945004 2306 16-17 -0.49078107008660155 2306 18-19 -0.4411689445718423 2306 20-21 -0.7095645376810644 2306 22-23 -1.183592206091678 2306 24-25 -0.14456317338009939 2306 26-27 -1.514063941563851 2306 28-29 -0.834742317218538 2306 30-31 -0.4632605981909066 2306 32-33 -0.6132813209005903 2306 34-35 -0.9464116811745384 2306 36-37 -0.566176551996687 2306 38-39 -0.5241217802574596 2306 40-41 -0.9108008999047783 2306 42-43 -0.3830295204684582 2306 44-45 -0.17766159872081033 2306 46-47 -0.2832250770303162 2306 48-49 -0.3960513440224389 2306 50-51 -0.4710467020618516 2306 52-53 -0.48434469858660734 2306 54-55 -0.27160001232052977 2306 56-57 -0.2549713220791858 2306 58-59 0.1654857939176857 2306 60-61 -0.019175432544397353 2306 62-63 -0.09135371831363415 2306 64-65 -0.1028684237701114 2306 66-67 -0.3849818285946647 2306 68-69 -0.6670256912638983 2306 70-71 -0.29547851791551594 2306 72-73 -1.0791966312625547 2306 74-75 -1.1568072591774516 2306 76-77 -1.0920732032303668 2306 78-79 -1.0288609512415263 2306 80-81 -0.7625160734779612 2306 82-83 -0.4989265308004498 2306 84-85 -0.4640959493547392 2306 86-87 -0.2876960799267927 2306 88-89 -0.13847458364215726 2306 90-91 -0.22734350001251613 2306 92-93 -0.27101386178107845 2306 94-95 -0.3175919842566586 2306 96-97 -0.2955508081962144 2306 98-99 -0.18831466160386867 2306 100-101 -0.4100899936813356 2307 1 -0.24069900802591349 2307 2 -0.20030836638632366 2307 3 -0.30795936169713656 2307 4 0.024141402266444345 2307 5 -0.11431034516848726 2307 6 -0.7045107980633709 2307 7 1.1144375500504111 2307 8 -0.8010263256075909 2307 9 0.04182603068343482 2307 10-11 -0.14189016329818216 2307 12-13 -0.1359281654682576 2307 14-15 0.5535758459348408 2307 16-17 0.2000690752337455 2307 18-19 0.2251099931711451 2307 20-21 0.004552006556103549 2307 22-23 -0.5293392573732589 2307 24-25 0.5502602231344085 2307 26-27 -0.4614939875038324 2307 28-29 -0.020325361067900616 2307 30-31 0.257304498261135 2307 32-33 0.21689615012361685 2307 34-35 -0.1019946276160475 2307 36-37 0.2365704104002262 2307 38-39 0.15889692570353375 2307 40-41 -0.10393609652862068 2307 42-43 0.3818645971929264 2307 44-45 0.5567239220885511 2307 46-47 0.32239905466997243 2307 48-49 0.3830560256123654 2307 50-51 0.29879969183847166 2307 52-53 0.3166289842461083 2307 54-55 0.3977899648982053 2307 56-57 0.3651249534097083 2307 58-59 0.7660247789765364 2307 60-61 0.6602951922904658 2307 62-63 0.6424984944884358 2307 64-65 0.6450944583578639 2307 66-67 0.46210876618987484 2307 68-69 0.04336134971680394 2307 70-71 0.4342846957644433 2307 72-73 -0.4063134409361311 2307 74-75 -0.43245588895332787 2307 76-77 -0.3851982481293028 2307 78-79 -0.347582421393934 2307 80-81 -0.1786398435515899 2307 82-83 0.18724993996684347 2307 84-85 0.3983916311437241 2307 86-87 0.47001671970890513 2307 88-89 0.4868521892721276 2307 90-91 0.393176804046103 2307 92-93 0.4337005414314312 2307 94-95 0.42165692812375966 2307 96-97 0.42362713951768427 2307 98-99 0.5020241685764901 2307 100-101 0.2642661860556359 2201 1 -0.2638735560807284 2201 2 -0.657347720739434 2201 3 -0.5824242898577268 2201 4 -0.33719940027374307 2201 5 -0.3856419757200804 2201 6 -0.6915840082770082 2201 7 0.9020257371636795 2201 8 -0.8754912243896129 2201 9 -0.21759773222918 2201 10-11 -0.3924070791832328 2201 12-13 -0.3452547881493331 2201 14-15 0.14988572900227126 2201 16-17 -0.11155048583871618 2201 18-19 -0.06519449951682432 2201 20-21 -0.23013210187196265 2201 22-23 -0.5416121537062892 2201 24-25 0.22551420819011625 2201 26-27 -0.46432615480184225 2201 28-29 -0.25237120073965613 2201 30-31 0.021507015593016376 2201 32-33 0.06408448906531561 2201 34-35 -0.18029904803274377 2201 36-37 -0.02063541760236376 2201 38-39 0.006150705403062062 2201 40-41 -0.31334604417014233 2201 42-43 0.1742112307910766 2201 44-45 0.4018407048299366 2201 46-47 0.14844609945054543 2201 48-49 0.1906469405255713 2201 50-51 0.03128808289774909 2201 52-53 0.08845938941767528 2201 54-55 0.2885044134536905 2201 56-57 0.41265095491036874 2201 58-59 0.6388315587859417 2201 60-61 0.44241595465252814 2201 62-63 0.41610769844091067 2201 64-65 0.336792141446562 2201 66-67 0.17155565737930445 2201 68-69 -0.029014696233623738 2201 70-71 0.20649019178922856 2201 72-73 -0.38665701299382604 2201 74-75 -0.28489707893805516 2201 76-77 -0.2883260225677162 2201 78-79 -0.27751604492874904 2201 80-81 -0.02272859678984318 2201 82-83 0.07970503188944278 2201 84-85 0.24134670220754018 2201 86-87 0.3615446240593414 2201 88-89 0.44169130930178824 2201 90-91 0.37329647470061644 2201 92-93 0.28435815861812586 2201 94-95 0.19245783486385193 2201 96-97 0.1922831538097718 2201 98-99 0.34659116816107627 2201 100-101 0.4124346013346063 1101 1 -0.5321205475704147 1101 2 -0.8805124846939449 1101 3 -0.7203303206464788 1101 4 -0.6527290301719049 1101 5 -0.7397102441655079 1101 6 -0.6860008700208304 1101 7 0.5006977771752723 1101 8 -0.8867468001496235 1101 9 -0.568035488614612 1101 10-11 -0.6469451846996925 1101 12-13 -0.5632000732838378 1101 14-15 -0.28260804856118327 1101 16-17 -0.47282579797656155 1101 18-19 -0.46867424652348433 1101 20-21 -0.5925339184895968 1101 22-23 -0.673381142081972 1101 24-25 -0.20169086981298534 1101 26-27 -0.7965283067340074 1101 28-29 -0.5108116557317679 1101 30-31 -0.20479262195018322 1101 32-33 -0.22769444387364146 1101 34-35 -0.47317946507405395 1101 36-37 -0.26188846298663293 1101 38-39 -0.35325094678507796 1101 40-41 -0.5762203588658004 1101 42-43 -0.27405309111142273 1101 44-45 -0.08229074283320514 1101 46-47 -0.2019367088684092 1101 48-49 -0.21442958346941765 1101 50-51 -0.26295134842915857 1101 52-53 -0.1489046806429748 1101 54-55 -0.09516368723079438 1101 56-57 -0.015423546650193742 1101 58-59 0.09881630326740876 1101 60-61 0.059590149077589416 1101 62-63 0.14999991466369522 1101 64-65 0.04586773733760907 1101 66-67 -0.049182164522196814 1101 68-69 -0.2934335354166713 1101 70-71 0.036717738298619906 1101 72-73 -0.4500033557714751 1101 74-75 -0.41026185288689376 1101 76-77 -0.4462459362100937 1101 78-79 -0.538730236846245 1101 80-81 -0.14360902101932638 1101 82-83 0.016226122581606717 1101 84-85 0.08538819262857089 1101 86-87 -0.04165827454447424 1101 88-89 0.09536719325893728 1101 90-91 -0.015288959555334714 1101 92-93 0.07251711984946496 1101 94-95 0.06827361493942874 1101 96-97 0.17034135411060802 1101 98-99 0.052040813718402745 1101 100-101 0.12054763842344585 2304 1 -0.3655693585047679 2304 2 -0.7387221815785026 2304 3 -0.7088304516332933 2304 4 -0.6150205497486994 2304 5 -0.6566139855694786 2304 6 -0.5823753122084128 2304 7 0.5461277370010826 2304 8 -0.7513235619063039 2304 9 -0.5235449014185214 2304 10-11 -0.49579790445444516 2304 12-13 -0.504559650592995 2304 14-15 -0.17138736457033943 2304 16-17 -0.3099522418912919 2304 18-19 -0.33557681514967896 2304 20-21 -0.4463109947168924 2304 22-23 -0.49309797153253854 2304 24-25 -0.04961795793103363 2304 26-27 -0.49946948000901514 2304 28-29 -0.44265032266882187 2304 30-31 -0.08248241334464979 2304 32-33 -0.14867373185117572 2304 34-35 -0.3412744323362986 2304 36-37 -0.19726581909559116 2304 38-39 -0.24489810390464228 2304 40-41 -0.4250276694319943 2304 42-43 -0.21771268696133816 2304 44-45 -0.07380197277159439 2304 46-47 -0.19999469112949342 2304 48-49 -0.048400839740661894 2304 50-51 -0.15108557274978196 2304 52-53 -0.08745936976323421 2304 54-55 0.0376081784758604 2304 56-57 0.03415858975210995 2304 58-59 0.08643285644732046 2304 60-61 0.03737454241561977 2304 62-63 0.0742944249196853 2304 64-65 0.13816764914599844 2304 66-67 0.021957640622854768 2304 68-69 -0.1499732862240819 2304 70-71 0.19409269222634862 2304 72-73 -0.24988749044010916 2304 74-75 -0.17939805507966256 2304 76-77 -0.08707491306278214 2304 78-79 -0.11477436217867165 2304 80-81 -0.240276203878647 2304 82-83 -0.016578836549168585 2304 84-85 0.07765504935980161 2304 86-87 0.15819756646085992 2304 88-89 0.09029647923284045 2304 90-91 0.1943819129843014 2304 92-93 0.16797902588625746 2304 94-95 0.1516263827237445 2304 96-97 0.19688265733067212 2304 98-99 0.16267951872120534 2304 100-101 0.07578379720106554 2202 1 -0.3026701759466661 2202 2 -0.80139411887669 2202 3 -0.5995690604794497 2202 4 -0.5077954266795679 2202 5 -0.5498282386227196 2202 6 -0.3075623381743 2202 7 0.6707845240271659 2202 8 -0.2380722638765569 2202 9 -0.40269476655046077 2202 10-11 -0.4511727635656193 2202 12-13 -0.4145385051392694 2202 14-15 -0.30062484321831207 2202 16-17 -0.4121404478975883 2202 18-19 -0.3900975637769619 2202 20-21 -0.4468072115596833 2202 22-23 -0.4792423328477966 2202 24-25 -0.19597940198526587 2202 26-27 -0.24955427060256952 2202 28-29 -0.30408526121585666 2202 30-31 -0.12823090094922662 2202 32-33 -0.10304280720585623 2202 34-35 -0.32933614612665707 2202 36-37 -0.21696990624626267 2202 38-39 -0.3099906972323687 2202 40-41 -0.3738986915739986 2202 42-43 -0.11573464165284264 2202 44-45 -0.09874143431677496 2202 46-47 -0.2365173093548094 2202 48-49 -0.15604837199181887 2202 50-51 -0.11927158922683034 2202 52-53 -0.2052290786875801 2202 54-55 -0.12852069205227323 2202 56-57 -4.2609218833433715E-4 2202 58-59 -0.012469350166494308 2202 60-61 -0.07622003475028905 2202 62-63 -0.02797908627540835 2202 64-65 0.013538503235896115 2202 66-67 -0.09533790554059962 2202 68-69 -0.19324239513037256 2202 70-71 -0.0352030680087303 2202 72-73 -0.12286844659335827 2202 74-75 -0.038598826913720075 2202 76-77 -0.05247870418886791 2202 78-79 -0.07073027283434996 2202 80-81 -0.013329526825977212 2202 82-83 -0.04101414178207108 2202 84-85 0.017189549442282015 2202 86-87 -0.003551863294095625 2202 88-89 0.21594814059623957 2202 90-91 0.1648356771897035 2202 92-93 0.094731244747436 2202 94-95 0.0806428206601737 2202 96-97 0.11792669168681513 2202 98-99 0.08003090582003836 2202 100-101 0.16117182112886397 1102 1 -0.19612584052572757 1102 2 -0.593415211332541 1102 3 -0.5840281417341942 1102 4 -0.6095142153287441 1102 5 -0.5326376258333703 1102 6 -0.17435259699934136 1102 7 -0.057290519586498334 1102 8 0.003678371336587105 1102 9 -0.5122968151359224 1102 10-11 -0.39382520691045286 1102 12-13 -0.30942363003352114 1102 14-15 -0.4433456427959648 1102 16-17 -0.3192667342627793 1102 18-19 -0.3499661505523157 1102 20-21 -0.3620253196284189 1102 22-23 -0.21744895156002286 1102 24-25 -0.17870075250222683 1102 26-27 -0.02979383832536442 1102 28-29 -0.2853289016297005 1102 30-31 -0.1522331967190098 1102 32-33 -0.19667721467205013 1102 34-35 -0.20761451190434954 1102 36-37 -0.14765387467914337 1102 38-39 -0.32010920882154537 1102 40-41 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-0.7856867611357004 1213 96-97 -0.8489928554618231 1213 98-99 -0.9373683940648903 1213 100-101 -0.846317369103236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3502.0 3 868.0 4 1320.0 5 760.0 6 1393.0 7 23491.0 8 178259.0 9 330212.0 10 307421.0 11 210656.0 12 130029.0 13 84660.0 14 56196.0 15 39872.0 16 31375.0 17 26768.0 18 23390.0 19 21475.0 20 20800.0 21 19990.0 22 19161.0 23 18780.0 24 20953.0 25 24034.0 26 28397.0 27 34035.0 28 39599.0 29 46635.0 30 58439.0 31 75464.0 32 102411.0 33 145967.0 34 222585.0 35 378435.0 36 728832.0 37 1105857.0 38 343790.0 39 641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.467327252154796 19.35875888711409 15.389668207596937 12.784245653134185 2 29.91613208206284 36.88212935524214 21.63238256877623 11.569355993918789 3 27.924621395610238 23.517808436267273 35.22642335563694 13.33114681248555 4 22.106344905952305 17.90876366623248 31.496001885406034 28.48888954240918 5 41.589574383602155 16.164787257162374 22.086254846948364 20.159383512287107 6 46.48444489472106 15.624798294686254 23.773856376829237 14.116900433763446 7 42.40498315640204 22.588514659321486 19.788531976696692 15.217970207579782 8 23.51942247184403 42.87875372063921 18.92308527982801 14.678738527688745 9 39.89041428154632 26.150120224969843 18.99942444732958 14.96004104615426 10-11 32.38881616768032 33.12492446611836 21.161143574802225 13.3251157913991 12-13 37.394805047622015 20.703632668542927 20.868330250762813 21.033232033072245 14-15 29.120600149694376 20.33764852409327 25.169228055025698 25.372523271186648 16-17 37.349858752750386 20.115243952056083 25.701874118066144 16.83302317712739 18-19 38.21678543350073 20.225501296905037 26.64136050529834 14.916352764295892 20-21 33.276219760016815 28.037316197695933 23.588123575627534 15.098340466659717 22-23 43.99198931909212 20.556542903150284 20.576791069742075 14.874676708015519 24-25 30.293571776297178 33.82633523323975 20.762868177666157 15.117224812796914 26-27 24.056879529610963 39.94684091148149 20.829931498538595 15.16634806036895 28-29 30.04428542310229 34.15751184273643 20.609165943390877 15.189036790770405 30-31 40.03665747351968 22.307234338810158 22.53788890838114 15.118219279289018 32-33 27.68116436801958 21.548186138285157 35.459178145592205 15.311471348103058 34-35 18.33299178285771 29.959836730355182 26.889817520057356 24.817353966729748 36-37 28.579680633798105 33.76837217221774 21.172168374952104 16.479778819032052 38-39 33.67183033210209 24.24000458789546 20.847670932584823 21.24049414741763 40-41 26.697877786944492 22.03420239895234 20.81636554529497 30.451554268808202 42-43 36.19926313603145 21.45827152661046 24.890169389537235 17.452295947820858 44-45 28.82649681278615 27.576116506283643 26.749573484212778 16.847813196717425 46-47 31.41961361771315 30.573509524065166 21.738735629539303 16.268141228682378 48-49 21.102368049050117 29.707842777827135 28.57447080630952 20.615318366813227 50-51 17.709920854040124 23.994580444608054 33.73163997791275 24.563858723439065 52-53 35.384293076376224 22.243355163018165 22.45168992352368 19.920661837081933 54-55 38.720196430497225 21.730236200108457 23.20745160154335 16.342115767850967 56-57 40.707789225726046 21.352683986964628 22.05983512623017 15.879691661079148 58-59 35.87885811131499 21.809380258551254 22.077812397691815 20.233949232441947 60-61 25.91662045826807 21.70906851534332 21.021896392678546 31.35241463371007 62-63 33.798916935634196 21.59562940035775 22.075938770237965 22.52951489377009 64-65 26.04305569749617 25.299468574348495 28.979989780275933 19.677485947879408 66-67 19.62610900197105 26.966001593395838 25.540786180893864 27.86710322373925 68-69 17.593653332417507 23.69159673279414 40.04177225229999 18.672977682488362 70-71 16.934418128017928 33.599402065791566 31.90273104855538 17.56344875763513 72-73 16.315838689748947 44.485643579787215 22.03797753601305 17.16054019445079 74-75 16.070147491106017 45.99935331357302 21.145746851287058 16.784752344033908 76-77 16.13122820744418 46.00000122638218 20.86733222427505 17.00143834189859 78-79 16.143379287289715 45.11536387253135 21.259331837043923 17.48192500313501 80-81 16.338690447472423 43.898199021826144 21.541361822108428 18.221748708592997 82-83 16.738278947768205 42.5534694752162 21.691809599013823 19.016441978001776 84-85 16.972863561318945 41.84275279721531 21.757515040303414 19.426868601162333 86-87 17.387820029514252 40.62660791078047 21.928639303026102 20.05693275667917 88-89 18.119163288832922 39.646715954364666 21.975623576681503 20.25849718012091 90-91 18.580308530733856 38.7549538138017 22.076249563984668 20.588488091479775 92-93 18.897505193404964 38.1654284823506 22.031359388119867 20.905706936124567 94-95 19.19569116177387 37.666054817852086 22.160250429811786 20.97800359056226 96-97 19.517653706752174 37.28977052684714 22.236814004841445 20.955761761559234 98-99 19.88573032290796 36.752736346170714 22.25991829032302 21.101615040598308 100-101 20.240390311480656 37.230740576309906 21.84859431908988 20.680274793119562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2311.0 1 1504.5 2 553.5 3 339.0 4 299.0 5 314.5 6 365.5 7 503.5 8 702.5 9 897.5 10 1071.0 11 1277.0 12 1403.5 13 1483.5 14 1489.5 15 1533.0 16 1611.0 17 1577.0 18 1653.0 19 1844.0 20 2176.5 21 2789.5 22 3622.5 23 4834.5 24 6577.0 25 8925.5 26 11742.5 27 14976.5 28 19199.5 29 24032.0 30 29423.5 31 36117.0 32 44951.5 33 56402.0 34 70886.5 35 87708.0 36 108194.5 37 131249.5 38 151429.0 39 165151.5 40 175792.0 41 184755.0 42 192434.0 43 201435.5 44 208544.5 45 215380.5 46 223795.5 47 230900.5 48 232361.0 49 227081.5 50 218520.0 51 210762.0 52 201195.0 53 188079.5 54 171312.5 55 151088.5 56 130083.0 57 107661.0 58 86839.0 59 70108.5 60 56514.5 61 45748.5 62 36791.5 63 29108.5 64 22981.5 65 18045.5 66 14254.0 67 11439.5 68 9115.0 69 7216.0 70 5753.5 71 4447.0 72 3439.0 73 2670.5 74 2062.0 75 1566.0 76 1210.5 77 963.5 78 710.5 79 520.5 80 376.0 81 295.5 82 252.0 83 188.5 84 136.5 85 108.0 86 93.5 87 78.0 88 56.0 89 42.0 90 37.5 91 29.5 92 21.5 93 17.5 94 16.5 95 15.0 96 13.0 97 10.0 98 6.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24469820554649263 2 0.23181720722020718 3 0.19941089813983712 4 0.20165284405105768 5 0.2157974846182129 6 0.2179986678765022 7 0.19639446182292214 8 0.19248124714151896 9 0.17428072260770106 10-11 0.16280603580754482 12-13 0.18943423883490554 14-15 0.18629551455919674 16-17 0.18727381822954756 18-19 0.17997730335484785 20-21 0.18140399620744277 22-23 0.14762194759064187 24-25 0.44125571798113994 26-27 0.30768669498855794 28-29 0.25740596259782017 30-31 0.26027972962947565 32-33 0.30029846414476286 34-35 0.29643620277952376 36-37 0.28877282402844257 38-39 0.31225211211686166 40-41 0.31727610909064224 42-43 0.3509358697486493 44-45 0.31917157245194694 46-47 0.3437208801798122 48-49 0.32162752229105673 50-51 0.34142798095242755 52-53 0.3311048390975801 54-55 0.30886881192356513 56-57 0.3430686777329117 58-59 0.3302386327227903 60-61 0.2988819619554008 62-63 0.27857197013238894 64-65 0.28436026684863114 66-67 0.2931751905450211 68-69 0.29936092312734336 70-71 0.27730832789151916 72-73 0.28441122016479525 74-75 0.2816291691022352 76-77 0.2856035277630353 78-79 0.28793718964335124 80-81 0.3034371884204716 82-83 0.3189168058711264 84-85 0.31442272338545246 86-87 0.3178365955684474 88-89 0.3133323224195407 90-91 0.28810024025507636 92-93 0.2744855141760278 94-95 0.2833717725150475 96-97 0.2708066847489795 98-99 0.29049504611478927 100-101 0.31145724038470163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4906452.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.61691384756688 #Duplication Level Percentage of deduplicated Percentage of total 1 94.52584055284726 76.20381544217732 2 2.863992087425191 4.617724067441397 3 0.935012043567922 2.2613335608825786 4 0.44871470786761675 1.4469597978519921 5 0.23029932245874807 0.928301031890496 6 0.19975682297998315 0.9662267153184584 7 0.13840683895308425 0.7810552548256401 8 0.10196651958980213 0.6576180900085856 9 0.07359600395086802 0.5339774439029087 >10 0.4299179506780439 6.534963526080443 >50 0.0345588764265461 1.9068763959977721 >100 0.017573568773851144 2.3062023176956807 >500 2.8716608790083065E-4 0.13542382628336427 >1k 5.236864166530863E-5 0.08341756187036287 >5k 0.0 0.0 >10k+ 2.5169751444074553E-5 0.6361049677730288 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 31349 0.6389342033713975 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006868507018921209 0.0 0.0 2 0.0073576588540966055 0.0 0.0 3 0.008111767933325343 0.0 0.0 4 0.008662063747897667 0.0 0.0 5 0.009823799356439235 0.0 0.0 6 0.010761340373858747 0.0 0.0 7 0.01222879587938494 0.0 0.0 8 0.014063115261292681 0.0 0.0 9 0.015204469543368609 0.0 0.0 10-11 0.02406015589268987 0.0 0.0 12-13 0.030898090921912615 0.0 0.0 14-15 0.036594671669059435 0.0 0.0 16-17 0.03909138416110053 0.0 0.0 18-19 0.04218934578387804 0.0 0.0 20-21 0.04573569658889968 0.0 0.0 22-23 0.04938395402624952 0.0 0.0 24-25 0.05355193528847322 0.0 0.0 26-27 0.05735305267431537 0.0 0.0 28-29 0.0625604815862868 0.0 0.0 30-31 0.1126781633652994 0.0 0.0 32-33 0.12106507920591092 0.0 0.0 34-35 0.1272915744411644 0.0 0.0 36-37 0.13461866130556255 2.038132646564157E-5 4.076265293128314E-5 38-39 0.14192536684349505 2.038132646564157E-5 6.11439793969247E-5 40-41 0.1495887455945763 2.038132646564157E-5 6.11439793969247E-5 42-43 0.15806737740428317 2.038132646564157E-5 6.11439793969247E-5 44-45 0.16800327405628343 2.038132646564157E-5 6.11439793969247E-5 46-47 0.17864232647134834 2.038132646564157E-5 6.11439793969247E-5 48-49 0.19086093168750046 2.038132646564157E-5 6.11439793969247E-5 50-51 0.20361964205499208 2.038132646564157E-5 6.11439793969247E-5 52-53 0.2174585627251627 2.038132646564157E-5 8.152530586256628E-5 54-55 0.23234712170831387 2.038132646564157E-5 8.152530586256628E-5 56-57 0.24790826446483122 2.038132646564157E-5 8.152530586256628E-5 58-59 0.26605783568248503 2.038132646564157E-5 8.152530586256628E-5 60-61 0.28617420490407325 2.038132646564157E-5 1.0190663232820784E-4 62-63 0.30666762866527586 2.038132646564157E-5 1.222879587938494E-4 64-65 0.3294335703273975 2.038132646564157E-5 1.222879587938494E-4 66-67 0.3533510569348278 2.038132646564157E-5 1.222879587938494E-4 68-69 0.3776557887451054 2.038132646564157E-5 1.222879587938494E-4 70-71 0.4054762993707062 2.038132646564157E-5 1.324786220266702E-4 72-73 0.43554894657076026 2.038132646564157E-5 1.6305061172513255E-4 74-75 0.46758839177474887 2.038132646564157E-5 1.6305061172513255E-4 76-77 0.5020226428384502 2.038132646564157E-5 1.6305061172513255E-4 78-79 0.5404312525629518 2.038132646564157E-5 1.6305061172513255E-4 80-81 0.5825594543674328 2.038132646564157E-5 1.6305061172513255E-4 82-83 0.6288352561076721 2.038132646564157E-5 1.6305061172513255E-4 84-85 0.6786675993161657 2.038132646564157E-5 1.6305061172513255E-4 86-87 0.7316284761371353 2.038132646564157E-5 1.6305061172513255E-4 88-89 0.7915393852828887 2.038132646564157E-5 1.6305061172513255E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 16130 0.0 89.80548 9 ATCGGGA 25725 0.0 87.46584 2 GAGAGCG 19600 0.0 86.44705 7 TCGGGAG 22985 0.0 85.88884 3 AGAGCGG 17975 0.0 83.388954 8 GATCGGG 27745 0.0 82.08269 1 GAGCGTC 31865 0.0 81.58571 9 GGATCGG 7105 0.0 80.817764 1 CGGGAGA 23660 0.0 80.31031 4 ATTGGGA 11155 0.0 78.73524 2 GGAGAGC 21880 0.0 78.53326 6 TCCGGAG 3680 0.0 78.38614 3 AGAGCGT 33825 0.0 77.70352 8 CGGAGAG 9545 0.0 77.34074 5 TCCGGAA 2545 0.0 76.61919 3 CGGAAGA 33765 0.0 76.49532 4 AAGAGCG 32520 0.0 76.461235 7 ATCCGGA 4760 0.0 75.2614 2 TCGGAAG 35215 0.0 75.231766 3 GAAGAGC 33505 0.0 74.59138 6 >>END_MODULE