##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3112678 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81298065524285 33.0 33.0 33.0 27.0 33.0 2 31.684057265158813 33.0 33.0 33.0 27.0 33.0 3 31.630290701447436 33.0 33.0 33.0 27.0 33.0 4 35.280760811108635 37.0 37.0 37.0 33.0 37.0 5 35.26283123406918 37.0 37.0 37.0 33.0 37.0 6 35.384973967753815 37.0 37.0 37.0 33.0 37.0 7 35.24107729742685 37.0 37.0 37.0 33.0 37.0 8 35.27787358666717 37.0 37.0 37.0 33.0 37.0 9 35.04573617958555 37.0 37.0 37.0 33.0 37.0 10-11 34.99157879485125 37.0 37.0 37.0 33.0 37.0 12-13 35.02432744408513 37.0 37.0 37.0 33.0 37.0 14-15 36.75118370740565 40.0 37.0 40.0 33.0 40.0 16-17 36.690958717862884 40.0 37.0 40.0 33.0 40.0 18-19 36.67634156183196 40.0 37.0 40.0 33.0 40.0 20-21 36.641493273637685 40.0 37.0 40.0 33.0 40.0 22-23 36.81850853188155 40.0 37.0 40.0 33.0 40.0 24-25 36.53284551116434 40.0 37.0 40.0 33.0 40.0 26-27 36.42548233386171 40.0 37.0 40.0 33.0 40.0 28-29 36.264444475143264 40.0 37.0 40.0 33.0 40.0 30-31 36.28427836094835 40.0 37.0 40.0 33.0 40.0 32-33 36.15805312981298 40.0 37.0 40.0 33.0 40.0 34-35 36.10346685394377 40.0 37.0 40.0 33.0 40.0 36-37 35.986810553484816 37.0 37.0 40.0 27.0 40.0 38-39 35.891746110583874 37.0 37.0 40.0 27.0 40.0 40-41 35.846824984788014 37.0 37.0 40.0 27.0 40.0 42-43 35.69932257689359 37.0 37.0 40.0 27.0 40.0 44-45 35.55675273831729 37.0 37.0 40.0 27.0 40.0 46-47 35.43934499488864 37.0 37.0 40.0 27.0 40.0 48-49 35.30543458076936 37.0 37.0 40.0 27.0 40.0 50-51 34.14121232584932 37.0 35.0 38.5 24.5 38.5 52-53 34.5797160837067 37.0 33.0 38.5 27.0 40.0 54-55 35.27164583037501 37.0 37.0 40.0 27.0 40.0 56-57 35.318974850594884 37.0 37.0 40.0 27.0 40.0 58-59 35.022242101495884 37.0 35.0 40.0 27.0 40.0 60-61 34.75404057213756 37.0 33.0 40.0 27.0 40.0 62-63 34.48378968206798 37.0 33.0 38.5 27.0 40.0 64-65 34.24264026025178 37.0 33.0 37.0 27.0 40.0 66-67 34.033652212018076 37.0 33.0 37.0 27.0 40.0 68-69 33.87325046149971 37.0 33.0 37.0 27.0 40.0 70-71 33.5421641750287 37.0 33.0 37.0 27.0 38.5 72-73 33.41388958318207 37.0 33.0 37.0 27.0 37.0 74-75 33.27866004771454 37.0 33.0 37.0 27.0 37.0 76-77 33.03935935551316 37.0 33.0 37.0 27.0 37.0 78-79 32.795453304196585 37.0 33.0 37.0 22.0 37.0 80-81 32.548489275151496 37.0 33.0 37.0 22.0 37.0 82-83 32.30765678300165 37.0 33.0 37.0 22.0 37.0 84-85 32.182215442779494 33.0 33.0 37.0 22.0 37.0 86-87 32.03775093344059 33.0 33.0 37.0 22.0 37.0 88-89 31.8341423365989 33.0 33.0 37.0 22.0 37.0 90-91 31.77804128791992 33.0 33.0 37.0 22.0 37.0 92-93 31.638218601474357 33.0 33.0 37.0 18.5 37.0 94-95 31.49699005165327 33.0 33.0 37.0 15.0 37.0 96-97 31.330707031051716 33.0 33.0 37.0 15.0 37.0 98-99 31.182526750277415 33.0 33.0 37.0 15.0 37.0 100-101 29.83668837573305 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.30005503785767473 2306 2 0.2917691596062433 2306 3 0.19746363238913034 2306 4 0.346188251650716 2306 5 0.3805554452262996 2306 6 0.3923340111185638 2306 7 0.46597428077221537 2306 8 0.3140423795629701 2306 9 0.36272767630136116 2306 10-11 0.37037929970396277 2306 12-13 0.4109324562547485 2306 14-15 0.5937072016167946 2306 16-17 0.5101560148324822 2306 18-19 0.5381172192120971 2306 20-21 0.5380031410789314 2306 22-23 0.40564035950387023 2306 24-25 0.529794894955181 2306 26-27 0.509033161348242 2306 28-29 0.47979484772923797 2306 30-31 0.5221185034101978 2306 32-33 0.4724944403890774 2306 34-35 0.3687058548305373 2306 36-37 0.40611944175729775 2306 38-39 0.38233963268122295 2306 40-41 0.47476707242058325 2306 42-43 0.34905568186889724 2306 44-45 0.3622289135540342 2306 46-47 0.4247489824147692 2306 48-49 0.34949513399639187 2306 50-51 0.30710129962137955 2306 52-53 0.28161055770245014 2306 54-55 0.2021061590856803 2306 56-57 0.3198229611199821 2306 58-59 0.339471349101629 2306 60-61 0.35306008639911823 2306 62-63 0.20650193116756554 2306 64-65 0.4464854634008617 2306 66-67 0.32859827301362543 2306 68-69 0.3732595354394803 2306 70-71 0.43683133208906355 2306 72-73 0.3515637961735294 2306 74-75 0.40530226004087666 2306 76-77 0.2239215237770864 2306 78-79 0.2790151655423756 2306 80-81 0.37303156609717547 2306 82-83 0.3898549471262953 2306 84-85 0.40267439590952847 2306 86-87 0.3754774215789638 2306 88-89 0.2856766867780429 2306 90-91 0.1583255006451516 2306 92-93 0.2992676308800277 2306 94-95 0.1811483527099611 2306 96-97 0.10036613410953521 2306 98-99 0.20177356840832417 2306 100-101 0.26783580923448014 2307 1 0.34280478124824754 2307 2 0.30061524943341666 2307 3 0.3149144604746823 2307 4 0.4156288431007624 2307 5 0.4283056047402667 2307 6 0.30468108577110087 2307 7 0.4920132021803738 2307 8 0.272939791275455 2307 9 0.37823867582975623 2307 10-11 0.2863033017013592 2307 12-13 0.2858596346607243 2307 14-15 0.47169808851445794 2307 16-17 0.46555625618405116 2307 18-19 0.4471474436413132 2307 20-21 0.3022276257794658 2307 22-23 0.24080174602702442 2307 24-25 0.37390293445916 2307 26-27 0.4655517343224105 2307 28-29 0.3406899985046081 2307 30-31 0.27915965801390286 2307 32-33 0.4188387963687674 2307 34-35 0.32790842369896467 2307 36-37 0.22601776894124725 2307 38-39 0.18176642270535837 2307 40-41 0.2585250827959271 2307 42-43 0.27962202580875584 2307 44-45 0.1792646585536204 2307 46-47 0.11123814933574039 2307 48-49 0.18807220004904224 2307 50-51 0.1392145822801254 2307 52-53 0.1716880953616169 2307 54-55 0.1794673125797388 2307 56-57 0.21589440950680228 2307 58-59 0.01332423561090934 2307 60-61 0.17542567854270885 2307 62-63 0.22350057183114558 2307 64-65 0.13141069989766407 2307 66-67 0.2061678349744014 2307 68-69 0.01203842133844546 2307 70-71 0.09935352533676678 2307 72-73 -0.11104231529206032 2307 74-75 0.07258039691773632 2307 76-77 0.05467200231898772 2307 78-79 0.05772247821748522 2307 80-81 -0.008796325266878569 2307 82-83 0.08776323353016124 2307 84-85 0.32188924041808775 2307 86-87 0.07570559590692483 2307 88-89 0.22670159869858608 2307 90-91 0.24258875417526227 2307 92-93 0.35984132633319277 2307 94-95 0.10381355369755596 2307 96-97 0.3774800409533796 2307 98-99 0.2503871839657883 2307 100-101 0.08889408038048074 2201 1 0.21424894410514383 2201 2 0.08502905087725665 2201 3 0.11581766544937011 2201 4 0.20046532374092862 2201 5 0.22001888824571125 2201 6 0.25506572681846507 2201 7 0.4610127065227374 2201 8 0.26955761302696146 2201 9 0.3389245956976481 2201 10-11 0.28586550554294377 2201 12-13 0.2474536148034403 2201 14-15 0.4155156382500067 2201 16-17 0.3871057016075028 2201 18-19 0.34025227701813776 2201 20-21 0.3144427268948107 2201 22-23 0.2799802203070385 2201 24-25 0.2971419051831887 2201 26-27 0.3273453179390202 2201 28-29 0.3152856830661861 2201 30-31 0.21265850346524218 2201 32-33 0.372456233270718 2201 34-35 0.2852620587400523 2201 36-37 0.21361962598930262 2201 38-39 0.3647313340767724 2201 40-41 0.22072329461569495 2201 42-43 0.3387546666511838 2201 44-45 0.4190767941827076 2201 46-47 0.24949848040167666 2201 48-49 0.3122674266039027 2201 50-51 0.3496027054415194 2201 52-53 0.3011606310436008 2201 54-55 0.41187615804297906 2201 56-57 0.49422247945648934 2201 58-59 0.5195418495674886 2201 60-61 0.4906973698194932 2201 62-63 0.39425849907670596 2201 64-65 0.29654094704732614 2201 66-67 0.27960976022303896 2201 68-69 0.3372739033952783 2201 70-71 0.3348060184589201 2201 72-73 0.16213377482707614 2201 74-75 0.44958834650363855 2201 76-77 0.36889697428930646 2201 78-79 0.15119393143562831 2201 80-81 0.3007633139039001 2201 82-83 0.31231490762573344 2201 84-85 0.32189661272965253 2201 86-87 0.19594799751114067 2201 88-89 0.48800417147149133 2201 90-91 0.3554321102778779 2201 92-93 0.2549832262031977 2201 94-95 0.3726962776460425 2201 96-97 0.3182791876120419 2201 98-99 0.18661907190903904 2201 100-101 0.11697372577080856 1101 1 0.255473408957144 1101 2 0.20475383815815817 1101 3 0.16456151943516062 1101 4 0.2401837090192558 1101 5 0.33610063120502076 1101 6 0.34015110731773035 1101 7 0.3287522167159125 1101 8 0.386305948979107 1101 9 0.26682754608085446 1101 10-11 0.22618771991320585 1101 12-13 0.2670860698514588 1101 14-15 0.47322074332484476 1101 16-17 0.5146291893778496 1101 18-19 0.4090505169755261 1101 20-21 0.3839465138289384 1101 22-23 0.3966844507520264 1101 24-25 0.37816096962986734 1101 26-27 0.40173380743195963 1101 28-29 0.39142272656889077 1101 30-31 0.3984116342858428 1101 32-33 0.4185563678736699 1101 34-35 0.29967270401701995 1101 36-37 0.37913460965332746 1101 38-39 0.2049262140836774 1101 40-41 0.1951006060089 1101 42-43 0.24869559632188043 1101 44-45 0.3632195334353199 1101 46-47 0.21927526983972712 1101 48-49 0.1462047583943118 1101 50-51 0.29539019198228544 1101 52-53 0.3861825701813899 1101 54-55 0.3724732859205844 1101 56-57 0.4945171042442382 1101 58-59 0.3831604940168347 1101 60-61 0.4325205122401954 1101 62-63 0.5079780922108128 1101 64-65 0.3120479493787869 1101 66-67 0.2980978008559987 1101 68-69 0.16502263269797623 1101 70-71 0.35698135005823417 1101 72-73 0.33089767600082354 1101 74-75 0.508130679957354 1101 76-77 0.6115205222830937 1101 78-79 0.4190164023235212 1101 80-81 0.3986061241346306 1101 82-83 0.5130155712407287 1101 84-85 0.5183396482442575 1101 86-87 0.5348093386883335 1101 88-89 0.589050664603807 1101 90-91 0.48050333185081584 1101 92-93 0.4183271603833809 1101 94-95 0.36129165202981994 1101 96-97 0.3868653682910619 1101 98-99 0.30408890338930306 1101 100-101 0.2392686291642221 2304 1 0.19949861466100316 2304 2 0.16856558484210638 2304 3 0.1350735215312291 2304 4 0.2827536300875124 2304 5 0.24035816530745535 2304 6 0.21837500623052364 2304 7 0.4181344302973429 2304 8 0.34820907797260503 2304 9 0.27018236667568374 2304 10-11 0.3792819133292298 2304 12-13 0.33401234800597734 2304 14-15 0.41932926115590874 2304 16-17 0.3584472073475169 2304 18-19 0.4187712742306289 2304 20-21 0.3957018036068831 2304 22-23 0.3880868802407136 2304 24-25 0.44341605060731837 2304 26-27 0.37347825465395346 2304 28-29 0.3740116465659753 2304 30-31 0.358752890581421 2304 32-33 0.4922111909669624 2304 34-35 0.3816503983862347 2304 36-37 0.456940458103162 2304 38-39 0.3097742061287718 2304 40-41 0.20208884615842493 2304 42-43 0.2073499904454792 2304 44-45 0.17476759582086743 2304 46-47 0.23207971769360114 2304 48-49 0.3286272593021593 2304 50-51 0.2597246339389798 2304 52-53 0.28974919208275196 2304 54-55 0.39520782030735546 2304 56-57 0.3375614565376779 2304 58-59 0.41240380073465843 2304 60-61 0.4052444512738873 2304 62-63 0.345400231567929 2304 64-65 0.3893322262052834 2304 66-67 0.3548535975981224 2304 68-69 0.37681078591669603 2304 70-71 0.4608687540407459 2304 72-73 0.3203903641496382 2304 74-75 0.34415248774535456 2304 76-77 0.40624563599310903 2304 78-79 0.3221925230018883 2304 80-81 0.2848372149325087 2304 82-83 0.3474075585892251 2304 84-85 0.42327441246360564 2304 86-87 0.3212970445756156 2304 88-89 0.3385940994044496 2304 90-91 0.4122218663951749 2304 92-93 0.4149733973409546 2304 94-95 0.35558622293916997 2304 96-97 0.3949570321739415 2304 98-99 0.24984690869742465 2304 100-101 0.21259695939341938 2202 1 0.2766448743669727 2202 2 0.20537429827986742 2202 3 0.299948874383972 2202 4 0.25235130282928253 2202 5 0.2960594812805013 2202 6 0.4003442769807606 2202 7 0.35211001079859017 2202 8 0.2654236493339468 2202 9 0.38717591105565674 2202 10-11 0.41902370563179403 2202 12-13 0.4244952990460362 2202 14-15 0.4187046754593311 2202 16-17 0.42340473063341477 2202 18-19 0.47695853290232293 2202 20-21 0.48522646609849573 2202 22-23 0.3624686045394938 2202 24-25 0.45660106993313576 2202 26-27 0.46973567607587796 2202 28-29 0.48746884476189223 2202 30-31 0.39315250521357115 2202 32-33 0.4422709679413117 2202 34-35 0.3964390605768173 2202 36-37 0.40026924758827676 2202 38-39 0.44479521786352194 2202 40-41 0.4809877696842122 2202 42-43 0.4362609842043952 2202 44-45 0.460116825048523 2202 46-47 0.3907538050288224 2202 48-49 0.3683010649171621 2202 50-51 0.2966747572435011 2202 52-53 0.3951248927487825 2202 54-55 0.4926033620910104 2202 56-57 0.5253792927749146 2202 58-59 0.4687784475764971 2202 60-61 0.4858919337628862 2202 62-63 0.6137515061381222 2202 64-65 0.5255171969080905 2202 66-67 0.46476754709812695 2202 68-69 0.5659919598714751 2202 70-71 0.5160873828573784 2202 72-73 0.43979874924773554 2202 74-75 0.5938099276880067 2202 76-77 0.5940531490046439 2202 78-79 0.5968212350567015 2202 80-81 0.5778952060498952 2202 82-83 0.6072303827159118 2202 84-85 0.5689767253304012 2202 86-87 0.41335321856093543 2202 88-89 0.7481468215230045 2202 90-91 0.806492366183452 2202 92-93 0.5034750220578594 2202 94-95 0.5549217859263607 2202 96-97 0.5917937637014141 2202 98-99 0.5279965737240211 2202 100-101 0.6564343357254501 1102 1 0.1664757179460068 1102 2 0.1577611013110669 1102 3 0.13844671791498797 1102 4 0.1120325351941105 1102 5 0.26923189617740917 1102 6 0.22315864553218034 1102 7 0.1513884575007154 1102 8 0.18580352869219752 1102 9 0.25002217708324537 1102 10-11 0.3199488783705604 1102 12-13 0.2664063749456531 1102 14-15 0.366403712227104 1102 16-17 0.271353425407149 1102 18-19 0.26812515390702174 1102 20-21 0.36266114358845414 1102 22-23 0.3110301582892774 1102 24-25 0.3289770364840905 1102 26-27 0.4131734388087409 1102 28-29 0.28558891315128676 1102 30-31 0.2972914334094199 1102 32-33 0.33343118685515805 1102 34-35 0.2120494293278341 1102 36-37 0.3713095543990619 1102 38-39 0.2651543139636132 1102 40-41 0.22505124264903742 1102 42-43 0.26584332780272746 1102 44-45 0.38562826266626615 1102 46-47 0.3805194037702435 1102 48-49 0.194259283742511 1102 50-51 0.25585754446906606 1102 52-53 0.23751989139775276 1102 54-55 0.5136372903480506 1102 56-57 0.4337033524913565 1102 58-59 0.4879427063979591 1102 60-61 0.4493319937165836 1102 62-63 0.4946437834277404 1102 64-65 0.5342019155879782 1102 66-67 0.621791182111167 1102 68-69 0.5723356787142464 1102 70-71 0.5245830986346647 1102 72-73 0.43145508312807124 1102 74-75 0.6446054580871134 1102 76-77 0.4299963935278015 1102 78-79 0.5190038208047056 1102 80-81 0.5119222482933381 1102 82-83 0.4774315213466025 1102 84-85 0.5562295034141655 1102 86-87 0.392761317017559 1102 88-89 0.4694230877483001 1102 90-91 0.5339288795671173 1102 92-93 0.5413912874169959 1102 94-95 0.5526040878169596 1102 96-97 0.5190941385061763 1102 98-99 0.5180257879709345 1102 100-101 0.46281286593719884 2305 1 0.21383060206989768 2305 2 0.2527673282982903 2305 3 0.22092316365897702 2305 4 0.32910124864440604 2305 5 0.21071671676798331 2305 6 0.36270678285078617 2305 7 0.23100635512543022 2305 8 0.3608667010263247 2305 9 0.19861072664645718 2305 10-11 0.38239924296517813 2305 12-13 0.3244294869953208 2305 14-15 0.42031475655603145 2305 16-17 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21764.0 12 13888.0 13 9995.0 14 7700.0 15 7001.0 16 7084.0 17 7666.0 18 8040.0 19 9012.0 20 10315.0 21 11416.0 22 13016.0 23 14251.0 24 16202.0 25 18765.0 26 22220.0 27 26607.0 28 32583.0 29 40575.0 30 51441.0 31 67226.0 32 92111.0 33 132869.0 34 204619.0 35 351371.0 36 675030.0 37 954046.0 38 219458.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.47127281605088 25.05960975619183 19.05157761732968 16.41753981042761 2 33.797720445399406 23.03772291869554 28.665308930144846 14.499247705760204 3 39.734651619184 19.641072725493327 23.070345779613785 17.553929875708892 4 33.48984821008042 18.454182456318826 29.069432403118796 18.98653693048196 5 35.883000316831485 16.91693348342044 27.950138967953652 19.249927231794427 6 32.04518531898938 17.37641027028054 31.464961672074466 19.113442738655614 7 36.27880325261877 17.97924942538356 24.631472885186163 21.110474436811508 8 31.68602396010276 25.554395652326466 22.513013502625554 20.24656688494522 9 31.253160071564455 23.70622526454486 24.098291380885698 20.942323283004985 10-11 25.296392318634837 28.477170273350445 28.51226285356257 17.714174554452146 12-13 25.9973118638672 25.997633757788023 28.112846995611136 19.89220738273364 14-15 25.387889307605217 26.05714874300608 28.282005042299467 20.272956907089235 16-17 25.593718716704917 26.10316768007107 28.75573359427558 19.54738000894843 18-19 25.149810166427844 26.0811147459938 28.66802363231638 20.101051455261974 20-21 24.221238396868923 27.1844302395942 28.163695878876833 20.430635484660048 22-23 25.095608808221385 26.534659932528843 27.727897758142174 20.6418335011076 24-25 23.945183019129786 26.959923313031943 28.340514667673666 20.754379000164604 26-27 23.144852436983456 27.447808854183876 28.75631615800326 20.65102255082941 28-29 23.340257450889556 27.949880256149395 27.82416048833069 20.88570180463035 30-31 23.607845007036744 27.915762676891116 27.908224171316082 20.56816814475606 32-33 23.441290255418245 27.611788891379526 28.274833054520133 20.6720877986821 34-35 22.71838280212521 27.466229820759857 28.149327948174076 21.66605942894086 36-37 21.365551458574625 27.988030026766797 28.818377634222315 21.828040880436262 38-39 21.337713890621448 28.230080793722166 28.93927013275442 21.492935182901967 40-41 22.05272842939244 27.442505323275242 29.267932196680498 21.236834050651822 42-43 22.95924964842587 27.15032603441476 28.702185999400847 21.188238317758515 44-45 22.08303346563778 27.712723389680257 28.687655390566757 21.516587754115204 46-47 22.614193580840222 28.131087269088855 28.378149176766637 20.87656997330429 48-49 21.60109438736406 28.214275294772627 28.79125611962181 21.393374198241492 50-51 21.500369602771737 27.807509909143015 27.957156710769105 22.734963777316146 52-53 23.152093766593943 27.05876113396089 27.566940788784322 22.222204310660842 54-55 23.86330799248139 26.919327386283253 27.94653682454859 21.270827796686763 56-57 24.381720638138983 27.092160367952722 27.010532466075166 21.51558652783313 58-59 23.622402781478755 27.532406442422193 27.029369277962328 21.81582149813672 60-61 22.409893867658482 27.3386340416343 27.070257482926323 23.18121460778089 62-63 23.139815477454423 27.116172257560812 27.58584023233356 22.158172032651205 64-65 22.5704097759619 27.79490948908473 28.295369572072072 21.339311162881298 66-67 22.47240167060351 27.712011519358754 27.774000906556516 22.04158590348122 68-69 22.698910181198148 26.935746150165812 29.160456476943857 21.20488719169218 70-71 22.229207303702875 28.2452098711768 28.3587774145428 21.16680541057752 72-73 22.124347798486067 29.55712892930114 27.048427941188553 21.27009533102424 74-75 22.49747405946477 29.07887234216356 26.921426694394146 21.502226903977526 76-77 22.179710686676053 28.64504819203341 26.740552524643835 22.434688596646698 78-79 21.887649950477734 28.71870981401315 27.193162946226085 22.200477289283032 80-81 21.36773582619099 28.83904076033706 27.661263498251802 22.131959915220147 82-83 20.91596246946437 28.758920529677507 27.79307917117166 22.532037829686466 84-85 20.942275135481523 29.33207253531932 27.47655908515963 22.24909324403952 86-87 20.784429963153134 29.26420544887463 27.733709071104727 22.217655516867502 88-89 21.15541601761917 29.082463750714187 27.697992055900418 22.064128175766225 90-91 21.664129662580876 28.932239513165914 26.922004163941544 22.48162666031167 92-93 21.644919123757198 28.89892249858147 26.863829997013116 22.592328380648215 94-95 21.94240790794855 29.179217789532974 26.785481814889145 22.092892487629328 96-97 21.960499641069855 29.84401037739861 26.601653269615177 21.593836711916353 98-99 22.187412586511158 29.42930996033603 26.808081550259296 21.575195902893523 100-101 22.338156343934802 29.480920240544005 26.496715671445624 21.684207744075565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1579.0 1 997.5 2 325.0 3 208.0 4 166.5 5 171.0 6 214.0 7 260.5 8 354.0 9 508.5 10 676.0 11 873.5 12 1032.0 13 1061.5 14 980.0 15 866.5 16 751.0 17 645.0 18 604.0 19 637.0 20 697.5 21 814.5 22 1015.5 23 1262.0 24 1587.0 25 2085.5 26 2821.0 27 3677.5 28 4561.0 29 5778.5 30 7445.5 31 9724.5 32 14180.5 33 22361.5 34 35945.5 35 55295.0 36 83818.0 37 116592.0 38 137360.0 39 141424.5 40 143462.0 41 147678.5 42 156693.0 43 172153.0 44 172551.5 45 164477.0 46 163680.0 47 160796.5 48 152314.0 49 137413.5 50 123763.5 51 120570.5 52 116317.0 53 105285.5 54 90929.5 55 75174.0 56 60911.5 57 46882.5 58 33931.5 59 24742.0 60 18749.0 61 14839.5 62 11818.0 63 9244.0 64 7083.5 65 5112.0 66 3601.0 67 2800.5 68 2270.5 69 1643.0 70 1110.5 71 860.0 72 706.5 73 523.5 74 395.5 75 305.0 76 249.0 77 196.0 78 154.5 79 130.5 80 106.0 81 84.5 82 69.5 83 66.5 84 53.5 85 44.0 86 32.5 87 23.0 88 24.5 89 21.0 90 18.0 91 12.0 92 6.5 93 7.5 94 9.0 95 5.5 96 1.5 97 1.5 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2538328731722331 2 0.2412713425545463 3 0.20631751822707006 4 0.2080844854495068 5 0.22250936331994509 6 0.22421207718883868 7 0.20104874323653138 8 0.1958763482763074 9 0.178270929405483 10-11 0.16652862904547144 12-13 0.19483223128123114 14-15 0.1914910568969871 16-17 0.1923745405082055 18-19 0.18459988472948374 20-21 0.18498540485074266 22-23 0.1552200388218762 24-25 0.4615960918540241 26-27 0.32192536458959137 28-29 0.27474733975052995 30-31 0.27675525704875353 32-33 0.3194516104781799 34-35 0.31578916932622003 36-37 0.30777356347171153 38-39 0.3319649510807093 40-41 0.3373140427631769 42-43 0.37347261746958726 44-45 0.3410407372686799 46-47 0.3672400421759013 48-49 0.3427434511375735 50-51 0.3654570116150787 52-53 0.3540038513460114 54-55 0.3290896135096531 56-57 0.36656538196369814 58-59 0.3539877880076256 60-61 0.31959618052365196 62-63 0.2958545663894563 64-65 0.30306700532467545 66-67 0.31251224829551916 68-69 0.31851993685180413 70-71 0.2968023033542178 72-73 0.3032276387085333 74-75 0.30130003810223865 76-77 0.30417537567329483 78-79 0.30611903961797526 80-81 0.3230016082614392 82-83 0.3393862134149436 84-85 0.3344387051921207 86-87 0.3377959429147506 88-89 0.33333033484350133 90-91 0.30868917375970145 92-93 0.29264189871229856 94-95 0.3010269613496802 96-97 0.28946135771191234 98-99 0.30873736377485883 100-101 0.330695947348232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3112678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.757247800326724 #Duplication Level Percentage of deduplicated Percentage of total 1 86.13392700926863 43.71921077224703 2 6.531916286093625 6.630841870884879 3 2.4962074510115997 3.8010186045605314 4 1.0646753941255584 2.1615997122616597 5 0.7602132163285958 1.9293165301136963 6 0.45258284717558295 1.378311583456108 7 0.295499719636185 1.049912674615064 8 0.27346661911206194 1.1104330361110795 9 0.19939567754230142 0.9108698233795771 >10 1.4724182366181096 14.867259958656076 >50 0.1745724163553397 6.191800268725204 >100 0.13838976896280322 13.217401517747728 >500 0.005141948093253725 1.8208712618899496 >1k 0.0015934096762931361 1.2111523853513388 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4584 0.1472686863209108 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4193 0.13470715570322406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012208137173199411 0.0 0.0 2 0.0013493204244062508 0.0 0.0 3 0.0015099538082641378 0.0 0.0 4 0.0017027138688936023 0.0 0.0 5 0.0019597272830662216 0.0 0.0 6 0.0022167406972388405 0.0 0.0 7 0.0025058807881830373 0.0 0.0 8 0.002859274232670389 0.0 0.0 9 0.003052034293299853 0.0 0.0 10-11 0.004995698237980286 0.0 0.0 12-13 0.006618095414944945 0.0 0.0 14-15 0.00827261926868118 0.0 0.0 16-17 0.009830763092102686 0.0 0.0 18-19 0.01185474372871206 0.0 0.0 20-21 0.014424877870438254 0.0 0.0 22-23 0.017219898749565487 0.0 0.0 24-25 0.020079172982235875 0.0 0.0 26-27 0.023548854073566234 0.0 0.0 28-29 0.028383918927688635 1.60633383857887E-5 0.0 30-31 0.06280765308843382 3.21266767715774E-5 0.0 32-33 0.0702128520842824 3.21266767715774E-5 0.0 34-35 0.07686307417599894 3.21266767715774E-5 0.0 36-37 0.08463772995472066 3.21266767715774E-5 0.0 38-39 0.09297460257694501 3.21266767715774E-5 0.0 40-41 0.10275717565389031 3.21266767715774E-5 0.0 42-43 0.11392119583201346 3.21266767715774E-5 0.0 44-45 0.12879584717725379 3.21266767715774E-5 0.0 46-47 0.14338135843154995 3.21266767715774E-5 0.0 48-49 0.16002297699922702 3.21266767715774E-5 0.0 50-51 0.17918653969347295 3.21266767715774E-5 0.0 52-53 0.2001491962869272 3.21266767715774E-5 0.0 54-55 0.22356954365340714 3.21266767715774E-5 0.0 56-57 0.25041138209606006 3.21266767715774E-5 0.0 58-59 0.28044982487748493 3.21266767715774E-5 0.0 60-61 0.3120303481439455 3.21266767715774E-5 0.0 62-63 0.3471448058552796 3.21266767715774E-5 0.0 64-65 0.38592170471857357 3.21266767715774E-5 0.0 66-67 0.4280397779661115 3.21266767715774E-5 0.0 68-69 0.4728725554008477 3.21266767715774E-5 0.0 70-71 0.5229098544725795 3.21266767715774E-5 0.0 72-73 0.5779749784590632 3.21266767715774E-5 0.0 74-75 0.6413448483909996 3.21266767715774E-5 0.0 76-77 0.7127142608390589 3.21266767715774E-5 0.0 78-79 0.7934646629044186 3.21266767715774E-5 0.0 80-81 0.882455557561688 3.21266767715774E-5 0.0 82-83 0.9796066281189381 3.21266767715774E-5 0.0 84-85 1.0959694513855913 3.21266767715774E-5 0.0 86-87 1.2292309066340945 3.21266767715774E-5 0.0 88-89 1.3756642993589443 3.21266767715774E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1075 0.0 63.98634 9 GGATCGG 810 0.0 55.0898 1 AGAGCGG 1295 0.0 54.9512 8 GATCGGG 2535 0.0 48.500946 1 ATCGGGA 2565 0.0 44.781685 2 TCGGGAA 730 0.0 44.208168 3 TTGTGTT 26135 0.0 43.484055 1 TCGGGAG 2220 0.0 40.19031 3 CGGGAGA 2155 0.0 40.08054 4 GAGAGCG 2075 0.0 38.641968 7 GGCGCCG 1395 0.0 37.11475 56-57 CGCCGGA 740 0.0 35.94108 58-59 TCTCGGG 1210 0.0 34.155144 48-49 GATCCGG 700 0.0 33.907856 1 GATCTCG 1905 0.0 33.54004 46-47 GCCGGAT 985 0.0 33.028522 58-59 ATCTCGG 2045 0.0 32.63768 46-47 GAATCGG 410 0.0 32.41922 1 TCGGAAA 985 0.0 31.799274 4 CCTATGT 1185 0.0 31.675901 36-37 >>END_MODULE