FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112678
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA45840.1472686863209108No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC41930.13470715570322406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10750.063.986349
GGATCGG8100.055.08981
AGAGCGG12950.054.95128
GATCGGG25350.048.5009461
ATCGGGA25650.044.7816852
TCGGGAA7300.044.2081683
TTGTGTT261350.043.4840551
TCGGGAG22200.040.190313
CGGGAGA21550.040.080544
GAGAGCG20750.038.6419687
GGCGCCG13950.037.1147556-57
CGCCGGA7400.035.9410858-59
TCTCGGG12100.034.15514448-49
GATCCGG7000.033.9078561
GATCTCG19050.033.5400446-47
GCCGGAT9850.033.02852258-59
ATCTCGG20450.032.6376846-47
GAATCGG4100.032.419221
TCGGAAA9850.031.7992744
CCTATGT11850.031.67590136-37
Produced by FastQC (version 0.11.2)