##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8534077 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64138922111905 33.0 33.0 33.0 27.0 33.0 2 31.41958421514125 33.0 33.0 33.0 27.0 33.0 3 31.338487688826806 33.0 33.0 33.0 27.0 33.0 4 34.90412730046846 37.0 37.0 37.0 33.0 37.0 5 34.8972127858701 37.0 37.0 37.0 33.0 37.0 6 35.15893704732216 37.0 37.0 37.0 33.0 37.0 7 34.88947123397176 37.0 37.0 37.0 33.0 37.0 8 35.00623699551809 37.0 37.0 37.0 33.0 37.0 9 34.67209002215471 37.0 37.0 37.0 33.0 37.0 10-11 34.63279584892426 37.0 37.0 37.0 33.0 37.0 12-13 34.65022913432817 37.0 37.0 37.0 33.0 37.0 14-15 36.30870087063897 40.0 37.0 40.0 33.0 40.0 16-17 36.253300269027335 40.0 37.0 40.0 33.0 40.0 18-19 36.25053869328809 40.0 37.0 40.0 33.0 40.0 20-21 36.216058690353975 40.0 37.0 40.0 33.0 40.0 22-23 36.57520561391701 40.0 37.0 40.0 33.0 40.0 24-25 36.16582443537831 40.0 37.0 40.0 27.0 40.0 26-27 36.05621240586416 40.0 37.0 40.0 27.0 40.0 28-29 35.84206130317315 40.0 37.0 40.0 27.0 40.0 30-31 35.84830105235751 40.0 37.0 40.0 27.0 40.0 32-33 35.66897375076414 40.0 37.0 40.0 27.0 40.0 34-35 35.771243275634845 40.0 37.0 40.0 27.0 40.0 36-37 35.52310074071279 38.5 37.0 40.0 27.0 40.0 38-39 35.460748127770586 37.0 37.0 40.0 27.0 40.0 40-41 35.68956701468712 37.0 37.0 40.0 27.0 40.0 42-43 35.405747217888944 37.0 37.0 40.0 27.0 40.0 44-45 35.11206050753937 37.0 37.0 40.0 22.0 40.0 46-47 34.993819952643975 37.0 37.0 40.0 22.0 40.0 48-49 34.864331901387814 37.0 37.0 40.0 22.0 40.0 50-51 33.81036648720183 37.0 35.0 38.5 22.0 38.5 52-53 34.232669684138074 37.0 33.0 38.5 22.0 40.0 54-55 34.91836832501043 37.0 37.0 40.0 24.5 40.0 56-57 35.05898077788611 37.0 37.0 40.0 24.5 40.0 58-59 34.72345515513863 37.0 35.0 40.0 22.0 40.0 60-61 34.45796557729676 37.0 33.0 40.0 22.0 40.0 62-63 34.161246494494954 37.0 33.0 40.0 22.0 40.0 64-65 33.91754574044739 37.0 33.0 38.5 22.0 40.0 66-67 33.72534446314464 37.0 33.0 37.0 22.0 40.0 68-69 33.61174395309534 37.0 33.0 37.0 22.0 40.0 70-71 33.25370523373529 37.0 33.0 37.0 22.0 40.0 72-73 33.22078626663434 37.0 33.0 37.0 22.0 38.5 74-75 33.11329743099341 37.0 33.0 37.0 22.0 37.0 76-77 32.85164505780766 37.0 33.0 37.0 22.0 37.0 78-79 32.49639791157263 37.0 33.0 37.0 22.0 37.0 80-81 32.142609271043604 37.0 33.0 37.0 22.0 37.0 82-83 31.82510416767976 35.0 33.0 37.0 15.0 37.0 84-85 31.642471470552703 33.0 33.0 37.0 15.0 37.0 86-87 31.465739470126646 33.0 33.0 37.0 15.0 37.0 88-89 31.2414972351433 33.0 33.0 37.0 15.0 37.0 90-91 31.15968094733619 33.0 33.0 37.0 15.0 37.0 92-93 31.00624894760148 33.0 33.0 37.0 15.0 37.0 94-95 30.861331049626106 33.0 33.0 37.0 15.0 37.0 96-97 30.70585026359617 33.0 27.0 37.0 15.0 37.0 98-99 30.563966788675565 33.0 27.0 37.0 15.0 37.0 100-101 29.29209626301708 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.20284537599864905 2306 2 0.20756351761825442 2306 3 0.07633430402583485 2306 4 0.23910061010863615 2306 5 0.2380667960420766 2306 6 0.18954670380564664 2306 7 0.40789532712255294 2306 8 0.1551186852890396 2306 9 0.2896924175663145 2306 10-11 0.22410911604551131 2306 12-13 0.24136702008613042 2306 14-15 0.4318088551695567 2306 16-17 0.32935608819920503 2306 18-19 0.4259329130849707 2306 20-21 0.37992657766567817 2306 22-23 0.26907639266900674 2306 24-25 0.49280656515335153 2306 26-27 0.2610470342130782 2306 28-29 0.2571618277604202 2306 30-31 0.2810854167237977 2306 32-33 0.3573546411093673 2306 34-35 0.19455827608790344 2306 36-37 0.3417073748485606 2306 38-39 0.2497446564941228 2306 40-41 0.06367653405535378 2306 42-43 0.2946825438488929 2306 44-45 0.36301739273170597 2306 46-47 0.35405739598548536 2306 48-49 0.35751154143950004 2306 50-51 0.32030006053641813 2306 52-53 0.2483241318456635 2306 54-55 0.29845490894157223 2306 56-57 0.2892649805435852 2306 58-59 0.44940620878035276 2306 60-61 0.42873220783894084 2306 62-63 0.3797271737731833 2306 64-65 0.4033668893975033 2306 66-67 0.3646427371960286 2306 68-69 0.2649224210701817 2306 70-71 0.32199511961351845 2306 72-73 0.04958558758232812 2306 74-75 0.13001182427364455 2306 76-77 0.1851989197367061 2306 78-79 0.1700850206609843 2306 80-81 0.1848967918842277 2306 82-83 0.2783858257452536 2306 84-85 0.33046107480530296 2306 86-87 0.2502507972778574 2306 88-89 0.3697925217911866 2306 90-91 0.26729469029146813 2306 92-93 0.2104479769875205 2306 94-95 0.08787623955572244 2306 96-97 0.14387200492683405 2306 98-99 0.23090978297011233 2306 100-101 0.06698821784081233 2307 1 0.2170971353346296 2307 2 0.2573778165108429 2307 3 0.2664969544284901 2307 4 0.39651831536484394 2307 5 0.41482798048416925 2307 6 0.293292587498037 2307 7 0.6024781746548697 2307 8 0.2921498937436553 2307 9 0.3900581353685979 2307 10-11 0.45723735227322493 2307 12-13 0.32185691140881545 2307 14-15 0.5625853304754074 2307 16-17 0.5353501879437559 2307 18-19 0.535280337376733 2307 20-21 0.4821359450832503 2307 22-23 0.36430477115119686 2307 24-25 0.5476447725614975 2307 26-27 0.4984312941159601 2307 28-29 0.4542836099143699 2307 30-31 0.4718340919570423 2307 32-33 0.5248617667058468 2307 34-35 0.46597424883402283 2307 36-37 0.5016618779227784 2307 38-39 0.3284309825779772 2307 40-41 0.22219873508461063 2307 42-43 0.4623979949265902 2307 44-45 0.5294323027398278 2307 46-47 0.40094311540246963 2307 48-49 0.3638057410937776 2307 50-51 0.24002804594972105 2307 52-53 0.3672779558797288 2307 54-55 0.32063964184523996 2307 56-57 0.30356918167457536 2307 58-59 0.4076292006637985 2307 60-61 0.441561525320175 2307 62-63 0.46084881237512576 2307 64-65 0.4444908594324133 2307 66-67 0.45033214110092956 2307 68-69 0.3921995071414557 2307 70-71 0.4083205701495487 2307 72-73 0.20578758029845545 2307 74-75 0.2542891212448595 2307 76-77 0.2806330749825463 2307 78-79 0.34613277013820465 2307 80-81 0.25794692510749684 2307 82-83 0.36229874775366966 2307 84-85 0.4079030345847876 2307 86-87 0.4070242948577629 2307 88-89 0.5283433765847221 2307 90-91 0.4536975960972498 2307 92-93 0.46571630494434757 2307 94-95 0.3538991001621845 2307 96-97 0.4557970631560231 2307 98-99 0.37328789196789813 2307 100-101 0.16337667473494477 2201 1 0.24426361999964286 2201 2 0.17691393874694228 2201 3 0.13557335174948193 2201 4 0.32597528904756956 2201 5 0.30918775042641045 2201 6 0.2526550329484607 2201 7 0.45588313923823165 2201 8 0.14068289331542871 2201 9 0.3203203255853708 2201 10-11 0.31845138597863354 2201 12-13 0.2907721551915827 2201 14-15 0.48599981330195163 2201 16-17 0.38715688085987665 2201 18-19 0.361056180832783 2201 20-21 0.35561056394015367 2201 22-23 0.30378260732766194 2201 24-25 0.4598545074812961 2201 26-27 0.36542228078059225 2201 28-29 0.3385585831413209 2201 30-31 0.352717076755404 2201 32-33 0.4160091175932905 2201 34-35 0.30498210378098634 2201 36-37 0.2954645957981725 2201 38-39 0.2928413080026857 2201 40-41 0.1992296111439984 2201 42-43 0.33049648382161223 2201 44-45 0.3899614159352751 2201 46-47 0.2112795153258773 2201 48-49 0.3416273238779368 2201 50-51 0.2692002032192775 2201 52-53 0.17801818620108634 2201 54-55 0.4123593094543878 2201 56-57 0.38922926057630747 2201 58-59 0.35563959548637314 2201 60-61 0.3576162279002375 2201 62-63 0.3210017129351215 2201 64-65 0.22636578319900025 2201 66-67 0.21141856996009523 2201 68-69 0.27142813404860533 2201 70-71 0.25672346070485474 2201 72-73 0.22466024120576833 2201 74-75 0.18064420683116822 2201 76-77 0.09052422385510539 2201 78-79 0.14851156410586697 2201 80-81 0.37453525557841516 2201 82-83 0.3643914911955619 2201 84-85 0.2815905079105825 2201 86-87 0.3785218892967315 2201 88-89 0.36583073292143453 2201 90-91 0.28099950964275067 2201 92-93 0.24860256601212072 2201 94-95 0.2120760187100359 2201 96-97 0.1574796378935197 2201 98-99 0.22072499955303826 2201 100-101 0.1625823735074725 1101 1 0.2539139954745231 1101 2 0.2545809463424007 1101 3 0.24173237222538546 1101 4 0.2881678062160873 1101 5 0.3431648938700036 1101 6 0.29666846513359957 1101 7 0.5336272724706319 1101 8 0.34344340779627913 1101 9 0.3827532326407521 1101 10-11 0.39018979608088955 1101 12-13 0.39936967910118426 1101 14-15 0.5664569729382052 1101 16-17 0.5457463018627848 1101 18-19 0.4916875584453422 1101 20-21 0.48160134438931834 1101 22-23 0.3951461806941694 1101 24-25 0.5155185927673003 1101 26-27 0.4445596424829432 1101 28-29 0.42390749715445963 1101 30-31 0.46722005169538505 1101 32-33 0.5595071654396975 1101 34-35 0.37010222635746004 1101 36-37 0.5398593141503412 1101 38-39 0.4318445452180768 1101 40-41 0.27825436546913096 1101 42-43 0.4015501928257308 1101 44-45 0.47723549944061006 1101 46-47 0.410227157171704 1101 48-49 0.3342229666851395 1101 50-51 0.3234355892597378 1101 52-53 0.4336727760646255 1101 54-55 0.46783867259004097 1101 56-57 0.46134314555933287 1101 58-59 0.5679776798934384 1101 60-61 0.571682632700842 1101 62-63 0.5932598603584651 1101 64-65 0.5648315641525414 1101 66-67 0.4609122095620961 1101 68-69 0.3362138225829625 1101 70-71 0.43629422375085625 1101 72-73 0.34688676646008787 1101 74-75 0.5103983652998991 1101 76-77 0.49504697251894214 1101 78-79 0.4564943306816289 1101 80-81 0.5639696573404791 1101 82-83 0.5921445606511782 1101 84-85 0.6993038566763552 1101 86-87 0.6411692657093084 1101 88-89 0.6523069816462375 1101 90-91 0.5743428895570126 1101 92-93 0.5333321394505504 1101 94-95 0.5901321522266407 1101 96-97 0.5034856504915091 1101 98-99 0.4716925677512265 1101 100-101 0.45491303262575045 2304 1 0.2237109502813901 2304 2 0.13092350140315645 2304 3 0.1475081665805611 2304 4 0.2839783105116638 2304 5 0.2697401520111029 2304 6 0.268146966720316 2304 7 0.3593837917845377 2304 8 0.19569775625431163 2304 9 0.3008018280116076 2304 10-11 0.3209214344569773 2304 12-13 0.2944832820271017 2304 14-15 0.471618237497907 2304 16-17 0.4146014381761134 2304 18-19 0.3698534740772601 2304 20-21 0.35106542560804144 2304 22-23 0.34831008051403956 2304 24-25 0.4755947181519318 2304 26-27 0.4959695656590952 2304 28-29 0.4622185302472275 2304 30-31 0.42531469241465913 2304 32-33 0.4360774789837052 2304 34-35 0.3240698352876592 2304 36-37 0.44917920098047404 2304 38-39 0.39198130655223196 2304 40-41 0.2041510443157719 2304 42-43 0.35741325083603925 2304 44-45 0.37629539782106036 2304 46-47 0.3233779655244504 2304 48-49 0.36980811894883203 2304 50-51 0.35271227994432053 2304 52-53 0.35900064446013147 2304 54-55 0.5158000119812058 2304 56-57 0.45945909854235367 2304 58-59 0.5070723629488043 2304 60-61 0.4369515121339589 2304 62-63 0.46106714808053084 2304 64-65 0.5678825704184476 2304 66-67 0.49124213174286524 2304 68-69 0.4904896750290746 2304 70-71 0.4472902158802228 2304 72-73 0.3489662846301087 2304 74-75 0.3986958977035755 2304 76-77 0.4840299234350951 2304 78-79 0.4875999163311846 2304 80-81 0.4679731933737443 2304 82-83 0.5555996556302603 2304 84-85 0.5485627447915924 2304 86-87 0.4850267672690407 2304 88-89 0.4956962512726939 2304 90-91 0.5386884664775771 2304 92-93 0.5474059513777938 2304 94-95 0.4864680179423715 2304 96-97 0.5918073948581046 2304 98-99 0.42601951840689267 2304 100-101 0.4114482266034294 2202 1 0.18397684712764573 2202 2 0.1099853951003773 2202 3 0.14181552658004293 2202 4 0.262478830564568 2202 5 0.277376698836143 2202 6 0.28189518990991047 2202 7 0.4554690983729657 2202 8 0.21106761038784327 2202 9 0.31276872567472935 2202 10-11 0.25518428833498774 2202 12-13 0.3141531487289342 2202 14-15 0.36892647890785923 2202 16-17 0.2945657191426534 2202 18-19 0.3600495867965492 2202 20-21 0.3710032849856191 2202 22-23 0.2316187139251582 2202 24-25 0.35518737150536595 2202 26-27 0.36053945541024746 2202 28-29 0.3603821369214302 2202 30-31 0.3052091370712944 2202 32-33 0.41173620340777717 2202 34-35 0.2725743001483423 2202 36-37 0.3968969433409697 2202 38-39 0.3379312502390732 2202 40-41 0.17836450501099677 2202 42-43 0.3278521089431621 2202 44-45 0.41503925577866596 2202 46-47 0.270090927336021 2202 48-49 0.316180716646393 2202 50-51 0.3557884454150013 2202 52-53 0.37194312243448735 2202 54-55 0.4001576704078573 2202 56-57 0.4213681656713675 2202 58-59 0.4179597974018563 2202 60-61 0.38732329333512894 2202 62-63 0.4359582837469773 2202 64-65 0.4289116352765774 2202 66-67 0.3717024096491883 2202 68-69 0.39366105770066895 2202 70-71 0.3607003343658377 2202 72-73 0.3220434804361645 2202 74-75 0.4296202447359292 2202 76-77 0.41294788475666167 2202 78-79 0.4542475639837207 2202 80-81 0.3901302356568763 2202 82-83 0.4412001098512519 2202 84-85 0.4933734350985439 2202 86-87 0.45800573147702295 2202 88-89 0.5745058423331741 2202 90-91 0.48930641419456933 2202 92-93 0.4561368983152221 2202 94-95 0.4184882176971634 2202 96-97 0.4356328195531738 2202 98-99 0.4439860841717511 2202 100-101 0.5099282950171009 1102 1 0.04157571905572155 1102 2 0.01735444970537614 1102 3 0.1219004371811856 1102 4 0.07027351840174845 1102 5 0.16546695172299053 1102 6 0.13781953935295377 1102 7 0.22396194103246359 1102 8 0.13314458273047336 1102 9 0.18826266351239695 1102 10-11 0.22217892032961117 1102 12-13 0.1601674405708735 1102 14-15 0.2525669874963157 1102 16-17 0.20549787502228156 1102 18-19 0.1997660040303515 1102 20-21 0.21282961598237193 1102 22-23 0.23287187950309374 1102 24-25 0.24475221924898705 1102 26-27 0.2934937424209991 1102 28-29 0.24436220526855834 1102 30-31 0.23482458125081251 1102 32-33 0.2575231847378916 1102 34-35 0.27939256099607945 1102 36-37 0.28748563144261396 1102 38-39 0.25067886386449345 1102 40-41 0.15868184433981725 1102 42-43 0.1255421440975013 1102 44-45 0.2528362306095957 1102 46-47 0.12084201222631918 1102 48-49 0.21151829461816618 1102 50-51 0.14113698751393144 1102 52-53 0.22598271674669945 1102 54-55 0.31948176276385 1102 56-57 0.3494543521057025 1102 58-59 0.3627474252217979 1102 60-61 0.3645344500375103 1102 62-63 0.4410675063021685 1102 64-65 0.4171006877334733 1102 66-67 0.43570114671013727 1102 68-69 0.4511562999032179 1102 70-71 0.42372818788521016 1102 72-73 0.3853807931180455 1102 74-75 0.4746248111789768 1102 76-77 0.42469498748640433 1102 78-79 0.37157256866812816 1102 80-81 0.3709015272977183 1102 82-83 0.3735341496869822 1102 84-85 0.4389629167379816 1102 86-87 0.32362361312755183 1102 88-89 0.49248952796436285 1102 90-91 0.47272323188718346 1102 92-93 0.3737829617367936 1102 94-95 0.37417960858715915 1102 96-97 0.33233786992395764 1102 98-99 0.3739225785152023 1102 100-101 0.3802068223234478 2305 1 0.28200247573784054 2305 2 0.256672315599328 2305 3 0.3133889744423328 2305 4 0.2836591428160702 2305 5 0.3060208796155379 2305 6 0.4135734105120008 2305 7 0.35884358891068047 2305 8 0.3755535619571049 2305 9 0.29590545839762683 2305 10-11 0.34049095969933774 2305 12-13 0.35896262033158166 2305 14-15 0.39165637007385357 2305 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-1.2940278555688245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6392.0 3 878.0 4 854.0 5 701.0 6 1318.0 7 7229.0 8 28479.0 9 73634.0 10 108884.0 11 87663.0 12 55339.0 13 38971.0 14 30067.0 15 25957.0 16 24389.0 17 24529.0 18 25967.0 19 28198.0 20 31209.0 21 35398.0 22 39626.0 23 42947.0 24 48561.0 25 55838.0 26 66254.0 27 79365.0 28 96906.0 29 118325.0 30 144418.0 31 186306.0 32 251627.0 33 355148.0 34 535933.0 35 876199.0 36 1617333.0 37 2625882.0 38 756153.0 39 1200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.41809840268683 27.90631721358583 17.340417883178237 17.335166500549104 2 32.210829428264574 27.00525227000659 25.382749206779543 15.40116909494929 3 34.402018090528735 22.61034274918687 24.29321212390219 18.694427036382205 4 30.648672559409174 21.046679863159483 26.51851554369069 21.786132033740653 5 33.374333295516124 19.951290964435145 25.84745195394005 20.82692378610868 6 31.938445276370842 19.859402481098144 27.72188211113558 20.480270131395432 7 33.50424323579813 20.8126741203831 23.252737255204032 22.430345388614736 8 29.061719445410183 26.455946207845425 22.55355632603445 21.928778020709938 9 28.71961119230837 24.660210017198168 25.32235702923688 21.29782176125658 10-11 24.612595957461796 29.60539474610888 28.293976101603402 17.488033194825928 12-13 25.033110327839207 27.016383741654902 28.765190243490064 19.18531568701582 14-15 23.82645773219019 27.057542258651957 29.646678848568957 19.469321160588894 16-17 25.084427761847582 26.91147346969086 29.67998551165774 18.324113256803813 18-19 24.919300024753998 26.713351562968228 30.078386882176538 18.288961530101233 20-21 24.233401001944706 28.317966670836398 29.170998559417583 18.277633767801312 22-23 25.860133610424302 27.255851727578857 28.249048563390133 18.63496609860671 24-25 23.777009958595567 28.7299969751448 28.57607605000411 18.91691701625552 26-27 22.92830076359272 29.76479877113255 28.736202998708844 18.570697466565893 28-29 23.428849167522564 29.327402741135394 28.641437385855056 18.602310705486982 30-31 24.473353588090806 28.098188258319603 29.030703395335326 18.397754758254266 32-33 22.393413245945055 29.389479527276645 29.792467333724478 18.424639893053822 34-35 24.63473407455729 28.373119230267157 28.814717383705794 18.177429311469755 36-37 22.764137097384175 28.84061958043577 30.1375389861878 18.25770433599225 38-39 22.710824625970293 29.56678475831226 29.076586662741384 18.645803952976067 40-41 25.09733378302216 27.50488954297698 28.25338981760501 19.144386856395847 42-43 24.879098710015743 27.40857958237399 28.602428546753938 19.109893160856334 44-45 23.22246171949847 28.255700393140295 29.381892830116268 19.13994505724497 46-47 23.48141094978685 28.757759526395006 28.633186057039172 19.127643466778977 48-49 21.671765340482352 28.98394263146833 29.576623410981917 19.7676686170674 50-51 21.341612038935274 28.25007143253232 29.92384783397568 20.484468694556725 52-53 24.02676665119108 27.9144258815793 28.03775431822982 20.0210531489998 54-55 24.678033290070744 27.83833214228463 28.11110453390709 19.37253003373753 56-57 24.871121463929622 27.509455405012762 28.1939045899253 19.425518541132313 58-59 24.125117263993992 27.747811047613226 28.18708027868775 19.939991409705033 60-61 22.006113400313694 27.95529065395045 27.976485655903318 22.06211028983254 62-63 23.169714735240575 27.957125225484052 28.18584569248703 20.687314346788344 64-65 21.854419407689726 28.500616148756748 29.42410175066876 20.220862692884765 66-67 21.39490372037957 28.140113483660627 28.893618942029107 21.57136385393069 68-69 21.026517313792446 27.822954352452594 31.220470487885848 19.93005784586911 70-71 20.746968455198328 30.112480232824147 29.635012330557302 19.50553898142022 72-73 20.753055865160768 31.723799553533805 27.8590802439121 19.664064337393324 74-75 20.950165305851527 31.61779479484756 27.68219388094206 19.749846018358856 76-77 21.116996137650148 31.343302265047633 27.734888898526837 19.804812698775383 78-79 20.98700684140897 31.086252553311667 28.0646689485272 19.862071656752164 80-81 20.635503110989006 31.347053273023295 27.855908363353453 20.161535252634245 82-83 20.578646606074226 31.068852534574948 27.810750342167722 20.541750517183107 84-85 20.82953156692307 30.69492655019628 28.043847375653947 20.4316945072267 86-87 20.888521918665077 30.366880734807904 28.597138416099554 20.14745893042746 88-89 21.195103360681497 30.642511963131653 28.203068905827717 19.959315770359133 90-91 21.427861148551003 30.189883036694592 27.831211003758828 20.551044810995577 92-93 21.24092080255616 30.33097144533349 27.655255392753002 20.772852359357348 94-95 20.87907864473642 30.327638507053567 27.657570905599965 21.135711942610047 96-97 20.872346228972464 30.65898570016496 27.72871879913398 20.739949271728598 98-99 21.26438916274802 30.333231934014904 27.59669627855882 20.80568262467826 100-101 21.54895661413781 30.259042375712962 27.145032833376874 21.046968176772353 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4487.0 1 2819.5 2 925.5 3 577.0 4 466.0 5 524.0 6 635.0 7 876.5 8 1252.0 9 1667.0 10 2071.0 11 2470.0 12 2757.0 13 2764.0 14 2545.0 15 2287.5 16 2043.0 17 1795.5 18 1757.0 19 1895.0 20 2210.0 21 2839.5 22 3749.0 23 5277.0 24 7412.5 25 10041.0 26 13774.0 27 18866.5 28 24876.0 29 31713.5 30 39724.0 31 48953.5 32 65426.5 33 94082.5 34 136203.5 35 197324.0 36 269763.5 37 351917.5 38 438155.0 39 488658.0 40 525910.0 41 533048.5 42 501908.0 43 497882.5 44 493120.5 45 488064.0 46 508195.5 47 486706.0 48 408770.0 49 333891.5 50 276385.5 51 231869.0 52 193987.5 53 159966.0 54 133564.0 55 110705.0 56 86428.0 57 64032.5 58 48185.0 59 37435.0 60 29359.0 61 22674.0 62 17226.0 63 13044.5 64 10000.5 65 7700.0 66 6003.0 67 4725.5 68 3631.5 69 2830.5 70 2296.0 71 1836.5 72 1418.5 73 1117.5 74 868.0 75 675.5 76 539.5 77 422.5 78 357.5 79 311.0 80 248.5 81 209.5 82 186.5 83 154.0 84 136.0 85 117.0 86 91.0 87 74.5 88 75.0 89 70.0 90 52.5 91 41.5 92 38.5 93 34.0 94 35.0 95 31.0 96 19.0 97 11.5 98 10.5 99 7.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2581767190523357 2 0.2460254342678183 3 0.20984108767708562 4 0.21300487445801108 5 0.22773405958254186 6 0.23086269317701258 7 0.207392082354073 8 0.2026698376403213 9 0.1842847211244989 10-11 0.17198110586534432 12-13 0.200338009605491 14-15 0.1968285498244274 16-17 0.19784799223161448 18-19 0.19054784717784948 20-21 0.19148526548330885 22-23 0.15712888458822202 24-25 0.4623288493881646 26-27 0.32086070936552363 28-29 0.27555411088978926 30-31 0.2790342763488073 32-33 0.3206908022976591 34-35 0.31663060926213815 36-37 0.30954138332710146 38-39 0.3343771095573663 40-41 0.3390700599490724 42-43 0.3749263101328943 44-45 0.34245062471313537 46-47 0.3686866195371802 48-49 0.34467113432419233 50-51 0.36674733541776106 52-53 0.35576196465065874 54-55 0.3312308993696682 56-57 0.36794254375722174 58-59 0.35498273568424565 60-61 0.3205326129586129 62-63 0.2980697268140421 64-65 0.30449104220643897 66-67 0.31371875365080487 68-69 0.32028068178902064 70-71 0.29689795393221785 72-73 0.3038407082570265 74-75 0.3018194000358797 76-77 0.30517652934230616 78-79 0.30763139352972796 80-81 0.32456351167208825 82-83 0.3411089447634466 84-85 0.3364218532361496 86-87 0.3397789825425761 88-89 0.33477551233718655 90-91 0.3089496380217802 92-93 0.2937048728292468 94-95 0.3026454999175658 96-97 0.29081059381114094 98-99 0.31003938680187676 100-101 0.33281865162454005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8534077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.00140688130781 #Duplication Level Percentage of deduplicated Percentage of total 1 89.74750443235861 45.77248990136697 2 4.8437843609347135 4.940796340746937 3 1.4934917518324071 2.2851054152724535 4 0.9663039622366032 1.9713144619619556 5 0.5308771468887536 1.3537740686230568 6 0.37012250329721136 1.1326061031953558 7 0.2814131210793172 1.0046725562933496 8 0.1661501837899801 0.6779114501501478 9 0.13757290906611547 0.6314770720013504 >10 1.1625835752416638 12.133286266531492 >50 0.17419029890545962 6.1671668553616605 >100 0.1057905350902669 10.26770738057617 >500 0.0117313098615728 4.156372386856466 >1k 0.008391368835560851 7.246590402928013 >5k 9.254058178651528E-5 0.25872933813462934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002706795357014004 0.0 0.0 2 0.002917714475742368 0.0 0.0 3 0.003187222238561944 0.0 1.1717728818242441E-5 4 0.0033395527131990957 0.0 1.1717728818242441E-5 5 0.0038902859676564904 0.0 1.1717728818242441E-5 6 0.004183229188112552 0.0 1.1717728818242441E-5 7 0.0049331638324800675 0.0 2.3435457636484882E-5 8 0.00542530844284625 0.0 5.8588644091212205E-5 9 0.006104936714304312 0.0 5.8588644091212205E-5 10-11 0.009684702868277377 0.0 5.8588644091212205E-5 12-13 0.012825054191566352 0.0 5.8588644091212205E-5 14-15 0.016978989057633297 0.0 5.8588644091212205E-5 16-17 0.020968875720244848 0.0 5.8588644091212205E-5 18-19 0.026546514637728252 0.0 6.444750850033342E-5 20-21 0.03215344787725726 0.0 7.030637290945465E-5 22-23 0.038979024913883484 0.0 7.030637290945465E-5 24-25 0.04698809256115219 0.0 8.20241017276971E-5 26-27 0.05633884015810966 0.0 8.20241017276971E-5 28-29 0.06754684777275854 0.0 8.20241017276971E-5 30-31 0.10204970027807342 0.0 8.20241017276971E-5 32-33 0.1168140385890589 0.0 8.20241017276971E-5 34-35 0.13065267632340322 0.0 8.20241017276971E-5 36-37 0.14473738636293065 0.0 8.788296613681832E-5 38-39 0.1609371464541508 0.0 9.374183054593953E-5 40-41 0.1780860425796486 0.0 9.374183054593953E-5 42-43 0.19664692502774464 0.0 9.374183054593953E-5 44-45 0.2159636009846173 0.0 9.374183054593953E-5 46-47 0.2355849378907643 0.0 9.374183054593953E-5 48-49 0.25678230932296486 0.0 9.374183054593953E-5 50-51 0.2786768856198508 0.0 9.374183054593953E-5 52-53 0.3016260575103787 0.0 9.374183054593953E-5 54-55 0.32566497818100304 0.0 9.374183054593953E-5 56-57 0.35016089027553887 0.0 9.374183054593953E-5 58-59 0.37624455462494655 0.0 9.960069495506075E-5 60-61 0.4037636407545889 0.0 1.0545955936418198E-4 62-63 0.4323548990711005 0.0 1.0545955936418198E-4 64-65 0.4614324431335691 0.0 1.0545955936418198E-4 66-67 0.4935858910108264 0.0 1.0545955936418198E-4 68-69 0.5263779551086778 0.0 1.0545955936418198E-4 70-71 0.5632243533776411 0.0 1.0545955936418198E-4 72-73 0.6013655606810203 0.0 1.0545955936418198E-4 74-75 0.6431158284604181 0.0 1.1717728818242441E-4 76-77 0.6876549156985576 0.0 1.1717728818242441E-4 78-79 0.7350531287683484 0.0 1.1717728818242441E-4 80-81 0.7876833077554843 0.0 1.2303615259154563E-4 82-83 0.8443561031849138 0.0 1.2889501700066684E-4 84-85 0.9067002793623727 0.0 1.3475388140978808E-4 86-87 0.9747685660675431 0.0 1.406127458189093E-4 88-89 1.0493050390803833 0.0 1.406127458189093E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4675 0.0 83.29603 9 CGGGAGA 6260 0.0 78.66844 4 AGAGCGG 5350 0.0 75.4495 8 ATTCGGA 1180 0.0 74.39063 2 GATCGGG 8360 0.0 73.170135 1 ATCGGGA 8510 0.0 69.69619 2 AAGCGGC 1550 0.0 68.54552 9 GAGAGCG 7215 0.0 68.11092 7 CGGAAAG 2330 0.0 67.39564 5 GATCCGG 1315 0.0 65.679955 1 TTCGGAG 1215 0.0 65.601395 3 TCGGGAA 1975 0.0 59.33486 3 AAAGCGG 1895 0.0 59.32254 8 GAACCGG 590 0.0 58.716217 1 GTTCGGA 1165 0.0 58.24232 2 ACCGGAA 590 0.0 56.289333 3 TCGGAGA 2170 0.0 54.439877 4 GGATCGG 3165 0.0 52.778397 1 TCGGAAA 2325 0.0 52.443016 4 ATCCGGA 1590 0.0 51.627075 2 >>END_MODULE